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https://www.readbyqxmd.com/read/29156776/uchl1-expression-and-localization-on-testicular-development-and-spermatogenesis-of-chinese-giant-salamanders
#1
Yuanxian Wang, Liqing Wang, Huihui Gao, Yao Gao, Changming Yang, Hong Ji, Wuzi Dong
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1), which is extensively expressed in vertebrates, is a deubiquitinating enzymes that inhibits the degradation of proteins by reversing ubiquitination modification. Herein, a 1087-bp sequence encoding UCHL1 was identified from the Chinese giant salamander (CGS; Andrias davidianus). The coding sequences (CDS) of UCHL1 encoded a putative poly peptide of 222 amino acids. The CGS UCHL1 isoforms were more related to their human and mouse counterparts. The phylogenic tree of vertebrate UCHL1 indicated that CGS UCHL1 has the closest relationship with human UCHL1 (up to 73...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156730/generation-of-patient-specific-human-neural-stem-cells-from-niemann-pick-disease-type-c-patient-derived-fibroblasts
#2
Eun-Ah Sung, Kyung-Rok Yu, Ji-Hee Shin, Yoojin Seo, Hyung-Sik Kim, Myung Guen Koog, Insung Kang, Jae-Jun Kim, Byung-Chul Lee, Tae-Hoon Shin, Jin Young Lee, Seunghee Lee, Tae-Wook Kang, Soon Won Choi, Kyung-Sun Kang
Niemann-Pick disease type C (NPC) is a neurodegenerative and lysosomal lipid storage disorder, characterized by the abnormal accumulation of unesterified cholesterol and glycolipids, which is caused by mutations in the NPC1 genes. Here, we report the generation of human induced neural stem cells from NPC patient-derived fibroblasts (NPC-iNSCs) using only two reprogramming factors SOX2 and HMGA2 without going through the pluripotent state. NPC-iNSCs were stably expandable and differentiated into neurons, astrocytes, and oligodendrocytes...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156709/using-molecular-functional-networks-to-manifest-connections-between-obesity-and-obesity-related-diseases
#3
Jialiang Yang, Jing Qiu, Kejing Wang, Lijuan Zhu, Jingjing Fan, Deyin Zheng, Xiaodi Meng, Jiasheng Yang, Lihong Peng, Yu Fu, Dahan Zhang, Shouneng Peng, Haiyun Huang, Yi Zhang
Obesity is a primary risk factor for many diseases such as certain cancers. In this study, we have developed three algorithms including a random-walk based method OBNet, a shortest-path based method OBsp and a direct-overlap method OBoverlap, to reveal obesity-disease connections at protein-interaction subnetworks corresponding to thousands of biological functions and pathways. Through literature mining, we also curated an obesity-associated disease list, by which we compared the methods. As a result, OBNet outperforms other two methods...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156593/site-directed-mutagenesis-of-the-fibronectin-domains-in-insulin-receptor-related-receptor
#4
Igor E Deyev, Natalia A Chachina, Egor S Zhevlenev, Alexander G Petrenko
The orphan insulin receptor-related receptor (IRR), in contrast to its close homologs, the insulin receptor (IR) and insulin-like growth factor receptor (IGF-IR) can be activated by mildly alkaline extracellular medium. We have previously demonstrated that IRR activation is defined by its extracellular region, involves multiple domains, and shows positive cooperativity with two synergistic sites. By the analyses of point mutants and chimeras of IRR with IR in, we now address the role of the fibronectin type III (FnIII) repeats in the IRR pH-sensing...
November 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29156585/pan-cellulosomics-of-mesophilic-clostridia-variations-on-a-theme
#5
Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A Bayer, Sarah Moraïs
The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides. Several cellulolytic bacteria have evolved various elaborate modular architectures of active cellulosomes. We present here a genome-wide analysis of a dozen mesophilic clostridia species, including both well-studied and yet-undescribed cellulosome-producing bacteria. We first report here, the presence of cellulosomal elements, thus expanding our knowledge regarding the prevalence of the cellulosomal paradigm in nature...
November 18, 2017: Microorganisms
https://www.readbyqxmd.com/read/29156553/inclusion-complexes-of-copaiba-copaifera-multijuga-hayne-oleoresin-and-cyclodextrins-physicochemical-characterization-and-anti-inflammatory-activity
#6
Jonas Gabriel de Oliveira Pinheiro, Emanuella de Aragão Tavares, Sofia Santos da Silva, Juliana Félix Silva, Yasmim Maria Barbosa Gomes de Carvalho, Magda Rhayanny Assunção Ferreira, Adriano Antunes de Souza Araújo, Euzébio Guimarães Barbosa, Matheus de Freitas Fernandes Pedrosa, Luiz Alberto Lira Soares, Eduardo Pereira de Azevedo, Valdir Florêncio da Veiga Júnior, Ádley Antonini Neves de Lima
Complexation with cyclodextrins (CDs) is a technique that has been extensively used to increase the aqueous solubility of oils and improve their stability. In addition, this technique has been used to convert oils into solid materials. This work aims to develop inclusion complexes of Copaifera multijuga oleoresin (CMO), which presents anti-inflammatory activity, with β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) by kneading (KND) and slurry (SL) methods. Physicochemical characterization was performed to verify the occurrence of interactions between CMO and the cyclodextrins...
November 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29156455/-the-overrated-impact-of-inhaled-substances-laba-lama-ics-on-exacerbation-rates-in-copd
#7
D Köhler, D Dellweg
Numerous studies have shown that exacerbation rates in COPD can be significantly reduced by long acting beta-2-agonists (LABA), long acting anticholinergic agents (LAMA) and inhaled steroids (ICS). Elaborate and extensive investigations however failed to prove that the reduction in exacerbation rates leads to life prolongation. As opposed to this, numerous studies have shown a reduction in life expectancy with increasing number and severity of exacerbations.This review aimed at comparing these studies and to elaborate the relevance and reduction of exacerbations rates by LABA, LAMA and ICS application through effect size calculation by means of Cohens' d...
November 20, 2017: Pneumologie
https://www.readbyqxmd.com/read/29156419/segmentation-of-the-hippocampus-by-transferring-algorithmic-knowledge-for-large-cohort-processing
#8
Benjamin Thyreau, Kazunori Sato, Hiroshi Fukuda, Yasuyuki Taki
The hippocampus is a particularly interesting target for neuroscience research studies due to its essential role within the human brain. In large human cohort studies, bilateral hippocampal structures are frequently identified and measured to gain insight into human behaviour or genomic variability in neuropsychiatric disorders of interest. Automatic segmentation is performed using various algorithms, with FreeSurfer being a popular option. In this manuscript, we present a method to segment the bilateral hippocampus using a deep-learned appearance model...
November 10, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/29156394/assessment-of-sampling-strategies-for-estimation-of-site-mean-concentrations-of-stormwater-pollutants
#9
David T McCarthy, Kefeng Zhang, Camilla Westerlund, Maria Viklander, Jean-Luc Bertrand-Krajewski, Tim D Fletcher, Ana Deletic
The estimation of stormwater pollutant concentrations is a primary requirement of integrated urban water management. In order to determine effective sampling strategies for estimating pollutant concentrations, data from extensive field measurements at seven different catchments was used. At all sites, 1-min resolution continuous flow measurements, as well as flow-weighted samples, were taken and analysed for total suspend solids (TSS), total nitrogen (TN) and Escherichia coli (E. coli). For each of these parameters, the data was used to calculate the Event Mean Concentrations (EMCs) for each event...
October 23, 2017: Water Research
https://www.readbyqxmd.com/read/29156375/downregulation-of-the-protein-synthesis-machinery-is-a-major-regulatory-event-during-early-adipogenic-differentiation-of-human-adipose-derived-stromal-cells
#10
Bruna H Marcon, Fabíola B Holetz, Guillermo Eastman, Ana Carolina Origa-Alves, Mariana Andrea Amorós, Alessandra Melo de Aguiar, Carmen K Rebelatto, Paulo R S Brofman, Jose Sotelo-Silveira, Bruno Dallagiovanna
Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event...
November 1, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29156366/triterpenoidal-saponins-from-the-fruits-of-gleditsia-caspica-with-proapoptotic-properties
#11
Usama Shaheen, Ehab A Ragab, Ashraf N Abdalla, Ammar Bader
Three previously undescribed oleanane-type triterpenoidal saponins named caspicaosides L-N were isolated from the fruits of Gleditsia caspica Desf. The aglycons of these saponins were echinocystic acid, erythrodiol and 12-oleanene-3,28,30-triol. Caspicaoside L is a bisdesmosidic saponin acylated with two monoterpenic acids. It has a disaccharide moiety made up of glucose and arabinose attached to C-3 and pentasaccharide moiety linked to C-28 made up of one glucose, 2 xyloses, one inner rhamnose and one terminal rhamnose which was acylated with two identical monoterpenic acids...
November 17, 2017: Phytochemistry
https://www.readbyqxmd.com/read/29156302/monitoring-the-effect-of-football-match-congestion-on-hamstring-strength-and-lower-limb-flexibility-potential-for-secondary-injury-prevention
#12
Martin Wollin, Kristian Thorborg, Tania Pizzari
OBJECTIVES: To investigate the effect of competitive football match congestion on hamstring strength and lower limb flexibility. DESIGN: Repeated measures. SETTING: Elite male youth football. PARTICIPANTS: Fifteen male elite youth football players from the national football association centre of excellence were included (age = 15.81 ±0.65 years, height = 171.95 ±6.89 cm, weight = 65.93 ±7.53 kg). MAIN OUTCOME MEASURES: Hamstring strength and pain, ankle dorsiflexion, hip extension, knee extension and flexion range of motion...
September 15, 2017: Physical Therapy in Sport
https://www.readbyqxmd.com/read/29156279/the-stocks-and-flows-of-nitrogen-phosphorus-and-potassium-across-a-30-year-time-series-for-agriculture-in-huantai-county-china
#13
Jessica Bellarby, Ben W J Surridge, Philip M Haygarth, Kun Liu, Giuseppina Siciliano, Laurence Smith, Clive Rahn, Fanqiao Meng
In order to improve the efficiency of nutrient use whilst also meeting projected changes in the demand for food within China, new nutrient management frameworks comprised of policy, practice and the means of delivering change are required. These frameworks should be underpinned by systemic analyses of the stocks and flows of nutrients within agricultural production. In this paper, a 30-year time series of the stocks and flows of nitrogen (N), phosphorus (P) and potassium (K) are reported for Huantai county, an exemplar area of intensive agricultural production in the North China Plain...
November 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29156269/autotrophic-denitrification-supported-by-biotite-dissolution-in-crystalline-aquifers-2-transient-mixing-and-denitrification-dynamic-during-long-term-pumping
#14
Clément Roques, Luc Aquilina, Alexandre Boisson, Virginie Vergnaud-Ayraud, Thierry Labasque, Laurent Longuevergne, Marc Laurencelle, Alexis Dufresne, Jean-Raynald de Dreuzy, Hélène Pauwels, Olivier Bour
We investigated the mixing and dynamic of denitrification processes induced by long-term pumping in the crystalline aquifer of Ploemeur (Brittany, France). Hydrological and geochemical parameters have been continuously recorded over 15 boreholes in 5km(2) on a 25-year period. This extensive spatial and temporal monitoring of conservative as well as reactive compounds is a key opportunity to identify aquifer-scale transport and reactive processes in crystalline aquifers. Time series analysis of the conservative elements recorded at the pumped well were used to determine mixing fractions from different compartments of the aquifer on the basis of a Principal Component Analysis approach coupled with an end-member mixing analysis...
November 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29156254/xenografted-tissue-models-for-the-study-of-human-endometrial-biology
#15
REVIEW
Satu Kuokkanen, Liyin Zhu, Jeffrey W Pollard
The human endometrium undergoes extensive morphological, biochemical and molecular changes under the influence of female sex steroid hormones. Besides the fact that estrogen stimulates endometrial cell proliferation and progesterone inhibits this proliferation and induces differentiation, there is limited knowledge about precise molecular mechanisms underlying human endometrial biology. The importance of paracrine signaling in endometrial physiology explains why in vitro culture of endometrial cells has been challenging...
November 10, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29156248/the-symbolic-violence-of-outbreak-a-mixed-methods-quasi-experimental-impact-evaluation-of-social-protection-on-ebola-survivor-wellbeing
#16
Eugene T Richardson, J Daniel Kelly, Osman Sesay, Michael D Drasher, Ishaan K Desai, Raphael Frankfurter, Paul E Farmer, Mohamed Bailor Barrie
Despite over 28,000 reported cases of Ebola virus disease (EVD) in the 2013-16 outbreak in West Africa, we are only beginning to trace the complex biosocial processes that have promoted its spread. Important questions remain, including the effects on survivors of clinical sequelae, loss of family and livelihood, and other psychological and social trauma. Another poorly understood question is what effect social protection and job creation programs have had on survivors' wellbeing. Several clinical and social protection programs have been developed to respond to the needs of EVD survivors; however, little in the way of impact evaluation has taken place...
November 11, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/29156140/automated-training-of-reaxff-reactive-force-fields-for-energetics-of-enzymatic-reactions
#17
Tomáš Trnka, Igor Tvaroska, Jaroslav Koca
Computational studies of the reaction mechanisms of various enzymes are nowadays based almost exclusively on hybrid QM/MM models. Unfortunately, the success of this approach strongly depends on the selection of the QM region, and computational cost is a crucial limiting factor. An interesting alternative is offered by empirical reactive molecular force fields, especially the ReaxFF potential developed by van Duin and co-workers. However, even though an initial parameterization of ReaxFF for biomolecules already exists, it does not provide the desired level of accuracy...
November 20, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/29156013/a-novel-strategy-to-initiate-a-peroral-endoscopic-myotomy-program
#18
J Matthew Reinersman, Dennis A Wigle, Christopher J Gostout, Louis M Wong Kee Song, Shanda H Blackmon
OBJECTIVES: The standard of care for achalasia remains laparoscopic Heller myotomy with partial fundoplication. Peroral endoscopic myotomy (POEM) has been introduced as an alternative, but safety and long-term comparative efficacy are not yet established. We report our experience in developing a POEM program using a novel hybrid approach. METHODS: We developed a hybrid approach to POEM with a POEM followed by laparoscopic evaluation, extension of the myotomy, if necessary, and partial fundoplication...
October 1, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29155905/adsorption-of-three-domain-antifreeze-proteins-on-ice-a-study-using-lgmmas-theory-and-monte-carlo-simulations
#19
Juan Ignacio Lopez Ortiz, Paola Torres, Evelina Quiroga, Claudio F Narambuena, Antonio J Ramirez-Pastor
In the present work, the adsorption of three-domain antifreeze proteins on ice is studied by combining a statistical thermodynamics based theory and Monte Carlo simulations. The three-domain protein is modeled by a trimer, and the ice surface is represented by a lattice of adsorption sites. The statistical theory, obtained from the exact partition function of non-interacting trimers adsorbed in one dimension and its extension to two dimensions, includes the configuration of the molecule in the adsorbed state, and allows the existence of multiple adsorption states for the protein...
November 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29155854/profiling-of-proteins-secreted-in-the-bovine-oviduct-reveals-diverse-functions-of-this-luminal-microenvironment
#20
Viju Vijayan Pillai, Darren M Weber, Brett S Phinney, Vimal Selvaraj
The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive success. Although previous studies have identified some of these factors involved in reproduction, knowledge of secreted proteins in the oviductal fluid remains rudimentary with limited definition of function even in extensively studied species like cattle...
2017: PloS One
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