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https://www.readbyqxmd.com/read/28320949/discovery-of-scmr-as-a-global-regulator-of-secondary-metabolism-and-virulence-in-burkholderia-thailandensis-e264
#1
Dainan Mao, Leah B Bushin, Kyuho Moon, Yihan Wu, Mohammad R Seyedsayamdost
Bacteria produce a diverse array of secondary metabolites that have been invaluable in the clinic and in research. These metabolites are synthesized by dedicated biosynthetic gene clusters (BGCs), which assemble architecturally complex molecules from simple building blocks. The majority of BGCs in a given bacterium are not expressed under normal laboratory growth conditions, and our understanding of how they are silenced is in its infancy. Here, we have addressed this question in the Gram-negative model bacterium Burkholderia thailandensis E264 using genetic, transcriptomic, metabolomic, and chemical approaches...
March 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28303005/ligand-co-crystallization-of-aminoacyl-trna-synthetases-from-infectious-disease-organisms
#2
Spencer O Moen, Thomas E Edwards, David M Dranow, Matthew C Clifton, Banumathi Sankaran, Wesley C Van Voorhis, Amit Sharma, Colin Manoil, Bart L Staker, Peter J Myler, Donald D Lorimer
Aminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28242693/manganese-scavenging-and-oxidative-stress-response-mediated-by-type-vi-secretion-system-in-burkholderia-thailandensis
#3
Meiru Si, Chao Zhao, Brianne Burkinshaw, Bing Zhang, Dawei Wei, Yao Wang, Tao G Dong, Xihui Shen
Type VI secretion system (T6SS) is a versatile protein export machinery widely distributed in Gram-negative bacteria. Known to translocate protein substrates to eukaryotic and prokaryotic target cells to cause cellular damage, the T6SS has been primarily recognized as a contact-dependent bacterial weapon for microbe-host and microbial interspecies competition. Here we report contact-independent functions of the T6SS for metal acquisition, bacteria competition, and resistance to oxidative stress. We demonstrate that the T6SS-4 in Burkholderia thailandensis is critical for survival under oxidative stress and is regulated by OxyR, a conserved oxidative stress regulator...
February 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28186697/complement-activated-vitronectin-enhances-the-invasion-of-non-phagocytic-cells-by-bacterial-pathogens-burkholderia-and-klebsiella
#4
Yi Han Tan, Akshamal Gamage, Yunn-Hwen Gan
Burkholderia pseudomallei is a serum-resistant Gram-negative bacterium capable of causing disseminated infections with metastatic complications. However, their interaction with non-phagocytic cells is poorly understood. We observed that exposure of B. pseudomallei and the closely related yet avirulent B. thailandensis to human plasma increased epithelial cell invasion by >20 fold. Enhanced invasion was primarily driven by a plasma factor which required a functional complement cascade, but surprisingly, was downstream of C3 mediated opsonisation...
February 10, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#5
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28050857/secondary-metabolites-from-bacillus-amyloliquefaciens-isolated-from-soil-can-kill-burkholderia-pseudomallei
#6
Patcharaporn Boottanun, Chotima Potisap, Julian G Hurdle, Rasana W Sermswan
Bacillus species are Gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. In this study, Bacillus amyloliquefaciens N2-4 and N3-8 were isolated from soil and their metabolites could kill Burkholderia pseudomallei, a Gram-negative pathogenic bacterium also found in soil in its endemic areas. Moreover, the metabolites were able to kill drug resistant isolates of B. pseudomallei and also inhibit other pathogenic bacteria such as Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii but not the non-pathogenic Burkholderia thailandensis, which is closely related to B...
December 2017: AMB Express
https://www.readbyqxmd.com/read/27986727/burkholderia-humptydooensis-sp-nov-a-new-species-related-to-burkholderia-thailandensis-and-the-fifth-member-of-the-burkholderia-pseudomallei-complex
#7
Apichai Tuanyok, Mark Mayo, Holger Scholz, Carina M Hall, Christopher J Allender, Mirjam Kaestli, Jennifer Ginther, Senanu Spring-Pearson, Molly C Bollig, Joshua K Stone, Erik W Settles, Joseph D Busch, Lindsay Sidak-Loftis, Jason W Sahl, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Jay E Gee, Richard A Bowen, Jason T Ladner, Sean Lovett, Galina Koroleva, Gustavo Palacios, David M Wagner, Bart J Currie, Paul Keim
During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43(T), MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses...
March 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27936381/a-proteomic-approach-for-rapid-identification-of-weissella-species-isolated-from-korean-fermented-foods-on-maldi-tof-ms-supplemented-with-an-in-house-database
#8
Eiseul Kim, Youngjae Cho, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Weissella are obligate heterofermentative lactic acid bacteria belonging to the Leuconostocaceae family. Some Weissella can be found in salted and fermented foods, such as kimchi and jeotgal, and plays an important role in the fermentation process. In the present study, for the first time, a rapid and accurate identification method for Weissella species from kimchi and jeotgal was developed based on MALDI-TOF MS, supplemented with an in-house database. Of the 135 Weissella spectra aligned with the MALDI bioTyper database, 56 isolates (41...
February 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27894256/burkholderia-pseudomallei-derived-mir-3473-enhances-nf-%C3%AE%C2%BAb-via-targeting-traf3-and-is-associated-with-different-inflammatory-responses-compared-to-burkholderia-thailandensis-in-murine-macrophages
#9
Yao Fang, Hai Chen, Yi Hu, Qian Li, Zhiqiang Hu, Tengfei Ma, Xuhu Mao
BACKGROUND: Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a kind of tropical disease. Burkholderia thailandensis (Bt), with a high sequence similarity to Bp, is thought to be an avirulent organism. Since there are numerous similarities between Bp and Bt, their differences in pathogenesis of host response and related mechanism are still undermined. In recent years, microRNAs have been researched in many diseases, but seldom involved in bacterial infection, bacteria-host interaction or explaining the differences between virulent and avirulent species...
November 28, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27862204/a-comparison-of-the-immunological-potency-of-burkholderia-lipopolysaccharides-in-endotoxemic-balb-c-mice
#10
Pei-Tan Hsueh, Chiu-Lin Liu, Hsuan-Han Wang, Wei-Fen Ni, Ya-Lei Chen, Jong-Kang Liu
Lipopolysaccharide is one of the virulence factors of the soil-borne pathogens Burkholderia pseudomallei, B. thailandensis, B. cenocepacia and B. multivorans, which cause septic melioidosis (often in B. pseudomallei infections but rarely in B. thailandensis infections) or cepacia syndromes (commonly in B. cenocepacia infections but rarely in B. multivorans infections). The inflammatory responses in Burkholderia LPS-induced endotoxemia were evaluated in this study. Prior to induction, the conserved structures and functions of each purified LPS were determined using electrophoretic phenotypes, the ratios of 3-hydroxytetradecanoic to 3-hydroxyhexadecanoic acid and endotoxin units...
November 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27825973/an-efficient-system-for-the-generation-of-marked-genetic-mutants-in-members-of-the-genus-burkholderia
#11
Sravanthi Shastri, Helena L Spiewak, Aderonke Sofoluwe, Vigdis A Eidsvaag, Atif H Asghar, Tyrone Pereira, Edward H Bull, Aaron T Butt, Mark S Thomas
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc)...
January 2017: Plasmid
https://www.readbyqxmd.com/read/27812452/efficacy-of-indirect-elisa-for-serodiagnosis-of-melioidosis-using-immunodominant-antigens-from-non-pathogenic-burkholderia-thailandensis
#12
Sumet Wajanarogana, Kanyanan Kritsiriwuthinan
Melioidosis caused by gram negative bacteria, B. pseudomallei, is a fatal disease in the tropical and sub-tropical regions. However, sporadic cases have been reported in elsewhere. Early detection is imperative to reduce the mortality rate. Serological tests have being substantially developed using recombinant proteins as specific targeted antigens to melioidosis antibodies. In the present study, we focus on a truncated flagellin fragment (FLAG300) and outer membrane protein A (OmpABT) of B. thailandensis E264 as potential antigens for developing indirect ELISA to improve the serodiagnosis of melioidosis...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27736903/a-quadruplex-real-time-pcr-assay-for-the-rapid-detection-and-differentiation-of-the-most-relevant-members-of-the-b-pseudomallei-complex-b-mallei-b-pseudomallei-and-b-thailandensis
#13
Chinn-Woan Lowe, Benjamin A Satterfield, Daniel B Nelson, Joseph D Thiriot, Michael J Heder, Jordon K March, David S Drake, Cynthia S Lew, Annette J Bunnell, Emily S Moore, Kim L O'Neill, Richard A Robison
The Burkholderia pseudomallei complex classically consisted of B. mallei, B. pseudomallei, and B. thailandensis, but has now expanded to include B. oklahomensis, B. humptydooensis, and three unassigned Burkholderia clades. Methods for detecting and differentiating the B. pseudomallei complex has been the topic of recent research due to phenotypic and genotypic similarities of these species. B. mallei and B. pseudomallei are recognized as CDC Tier 1 select agents, and are the causative agents of glanders and melioidosis, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27701356/macropsini-hemiptera-cicadellidae-of-thailand-with-description-of-two-new-species-and-three-new-country-records
#14
Liyuan Yang, Christopher H Dietrich, Yalin Zhang
The fauna of Thailand was previously known to include only a single species of Macropsini. Study of an extensive collection of recent Malaise trap samples from Thailand revealed the presence of five additional species of this leafhopper tribe, including two new species, Pediopsis thailandensis and Varicopsella odontoida spp. nov., described and illustrated herein; and three additional species, Macropsis hainanensis, P. rufoscutellata and V. elegans, previously recorded from neighboring countries. A key to genera and species of the Macropsini of Thailand is provided...
September 14, 2016: Zootaxa
https://www.readbyqxmd.com/read/27667932/sinocoelotes-gen-n-a-new-genus-of-the-subfamily-coelotinae-araneae-agelenidae-from-southeast-asia
#15
Lu Chen, Zhe Zhao, Shuqiang Li
A new genus of the spider subfamily Coelotinae, Sinocoelotes gen. n., with nine new species, is described from Yunnan and Sichuan Provinces in southern China. The new species are: Sinocoelotes cangshanensis sp. n. (♀), Sinocoelotes hehuaensis sp. n. (♂♀), Sinocoelotes luoshuiensis sp. n. (♀), Sinocoelotes mangbangensis sp. n. (♀) from Yunnan; Sinocoelotes kangdingensis sp. n. (♀), Sinocoelotes ludingensis sp. n. (♂♀), Sinocoelotes mahuanggouensis sp. n. (♀), Sinocoelotes muliensis sp. n. (♀), and Sinocoelotes yanyuanensis sp...
2016: ZooKeys
https://www.readbyqxmd.com/read/27632710/characterization-of-secreted-sphingosine-1-phosphate-lyases-required-for-virulence-and-intracellular-survival-of-burkholderia-pseudomallei
#16
Rafael Custódio, Christopher J McLean, Andrew E Scott, Jonathan Lowther, Amanda Kennedy, David J Clarke, Dominic J Campopiano, Mitali Sarkar-Tyson, Alan R Brown
Sphingosine-1-phosphate (S1P), a bioactive sphingolipid metabolite, plays a critical role in the orchestration of immune responses. S1P levels within the mammalian host are tightly regulated, in part through the activity of S1P lyase (S1PL) which catalyses its irreversible degradation. Herein, we describe the identification and characterization of secreted S1PL orthologues encoded by the facultative intracellular bacteria Burkholderia pseudomallei and Burkholderia thailandensis. These bacterial orthologues exhibited S1PL enzymatic activity, functionally complemented an S1PL-deficient yeast strain and conferred resistance to the antimicrobial sphingolipid D-erythro-sphingosine...
December 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27566409/fervidobacterium-thailandense-sp-nov-an-extremely-thermophilic-bacterium-isolated-from-a-hot-spring
#17
Wirojne Kanoksilapatham, Patlada Pasomsup, Porranee Keawram, Alba Cuecas, Maria Carmen Portillo, Juan M Gonzalez
Strain FC2004T, a strictly anaerobic, extremely thermophilic heterotroph, was isolated from a hot spring in Thailand. Typical cells of strain FC2004T were rod shaped (0.5-0.6×1.1-2.5 µm) with an outer membrane swelling out over an end. Filaments (10-30 µm long) and membrane-bound spheroids containing two or more cells inside (3-8 µm in diameter) were observed. The temperature range for growth was 60-88°C (optimum 78-80°C), pH range was 6.5-8.5 (optimum pH 7.5) and NaCl concentration range was 0 to <5 g l-1 (optimum 0...
December 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27561257/nonomuraea-purpurea-sp-nov-an-actinomycete-isolated-from-mangrove-sediment
#18
Paweena Suksaard, Ratchanee Mingma, Nantana Srisuk, Atsuko Matsumoto, Yoko Takahashi, Kannika Duangmal
A polyphasic approach was used to verify the novel actinomycete, strain 1SM4-01T, isolated from mangrove sediment collected from Ranong Province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism was a member of the genus Nonomuraea and was most closely related to Nonomuraea syzygii GKU 164T (98.7 % sequence similarity), Nonomuraea rhizophila YIM 67092T (98.4 %), Nonomuraea solani NEAU-Z6T (98.4 %), Nonomuraea monospora PT708T (98.3 %) and Nonomuraea thailandensis KC-061T (98...
December 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27527221/modulation-of-human-airway-barrier-functions-during-burkholderia-thailandensis-and-francisella-tularensis-infection-running-title-airway-barrier-functions-during-bacterial-infections
#19
Cornelia Blume, Jonathan David, Rachel E Bell, Jay R Laver, Robert C Read, Graeme C Clark, Donna E Davies, Emily J Swindle
The bronchial epithelium provides protection against pathogens from the inhaled environment through the formation of a highly-regulated barrier. In order to understand the pulmonary diseases melioidosis and tularemia caused by Burkholderia thailandensis and Fransicella tularensis, respectively, the barrier function of the human bronchial epithelium were analysed. Polarised 16HBE14o- or differentiated primary human bronchial epithelial cells (BECs) were exposed to increasing multiplicities of infection (MOI) of B...
August 3, 2016: Pathogens
https://www.readbyqxmd.com/read/27517336/burkholderia-thailandensis-growth-and-laboratory-maintenance
#20
Erin C Garcia, Peggy A Cotter
Burkholderia thailandensis is a nonpathogenic Gram-negative bacterium found in tropical soils. Closely related to several human pathogens, its ease of genetic manipulation, rapid growth in the laboratory, and low virulence make B. thailandensis a commonly used model organism. This unit describes the fundamental protocols for in vitro growth and maintenance of B. thailandensis in the laboratory. © 2016 by John Wiley & Sons, Inc.
August 12, 2016: Current Protocols in Microbiology
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