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https://www.readbyqxmd.com/read/29752487/fatty-acid-synthesis-pathway-provides-lipid-precursors-for-rhamnolipid-biosynthesis-in-burkholderia-thailandensis-e264
#1
Victor U Irorere, Thomas J Smyth, Diego Cobice, Stephen McClean, Roger Marchant, Ibrahim M Banat
Rhamnolipid production was monitored for a period of 216 h using different substrates in Pseudomonas aeruginosa PAO1 and Burkholderia thailandensis E264 which showed comparable crude yields attained by both after 216 h. The crude yield for P. aeruginosa, however, was significantly higher at the early stages of fermentation (72 or 144 h). Additionally, P. aeruginosa produced rhamnolipid with odd and even carbon chain lipid moieties using odd carbon chain fatty acid substrates (up to 45.97 and 67.57%, respectively)...
May 12, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29735757/malleilactone-is-a-burkholderia-pseudomallei-virulence-factor-regulated-by-antibiotics-and-quorum-sensing
#2
Jennifer R Klaus, Jacqueline Deay, Benjamin Neuenswander, Wyatt Hursh, Zhe Gao, Tiffany Bouddhara, Todd D Williams, Justin Douglas, Kyle Monize, Patricia Martins, Charlotte Majerczyk, Mohammad R Seyedsayamdost, Blake R Peterson, Mario Rivera, Josephine R Chandler
Burkholderia pseudomallei , the causative agent of melioidosis, encodes almost a dozen predicted polyketide (PK) biosynthetic gene clusters. Many of these are regulated by LuxR-I-type acyl-homoserine (AHL) quorum-sensing systems. One of the PK gene clusters, the mal gene cluster, is conserved in the close relative Burkholderia thailandensis. The B. thailandensis mal genes code for the cytotoxin malleilactone and are regulated by a genetically linked LuxR-type transcription factor, MalR. Although AHLs typically interact with LuxR-type proteins to modulate gene transcription, the B...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29721827/burkholderia-alba-sp-nov-isolated-from-a-soil-sample-on-halla-mountain-in-jeju-island
#3
Jae-Won Lee, Ye-Eun Kim, Soo-Je Park
A rod-shaped, round and white colony-forming strain AD18T was isolated from the soil on Halla mountain in Jeju Island, Republic of Korea. Comparative analysis of 16S rRNA gene sequence revealed that this strain was closely related to Burkholderia oklahomensis C6786T (98.8%), Burkholderia thailandensis KCTC 23190T (98.5%). DNA-DNA relatedness (14.6%) indicated that the strain AD18T represents a distinct species that is separate from B. oklahomensis C6786T . The isolate grew at pH 5.0-9.0 (optimum, pH 7.0), 0-3% (w/v) NaCl (optimum, 0%), and temperature 10-40°C (optimum 35°C)...
May 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29717997/crystal-structure-of-chorismate-mutase-from-burkholderia-thailandensis
#4
Oluwatoyin A Asojo, David M Dranow, Dmitry Serbzhinskiy, Sandhya Subramanian, Bart Staker, Thomas E Edwards, Peter J Myler
Burkholderia thailandensis is often used as a model for more virulent members of this genus of proteobacteria that are highly antibiotic-resistant and are potential agents of biological warfare that are infective by inhalation. As part of ongoing efforts to identify potential targets for the development of rational therapeutics, the structures of enzymes that are absent in humans, including that of chorismate mutase from B. thailandensis, have been determined by the Seattle Structural Genomics Center for Infectious Disease...
May 1, 2018: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29696980/enantioselective-synthesis-of-thailanstatin-a-methyl-ester-and-evaluation-of-in-vitro-splicing-inhibition
#5
Arun K Ghosh, Anne M Veitschegger, Shenyou Nie, Nicola Relitti, Andrew J MacRae, Melissa S Jurica
Thailanstatin A has been isolated recently from the fermentation broth of B. thailandensis MSMB43. We describe here an enantioselective convergent synthesis of thailanstatin A methyl ester and evaluation of its splicing activity. Synthesis of both highly functionalized tetrahydropyran rings were carried out from commercially available tri- O-acetyl-d-glucal as the key starting material. Our convergent synthesis involved the synthesis of both tetrahydropyran fragments in a highly stereoselective manner. The fragments were then coupled using cross-metathesis as the key step...
April 26, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29608197/air-sampling-to-assess-potential-generation-of-aerosolized-viable-bacteria-during-flow-cytometric-analysis-of-unfixed-bacterial-suspensions
#6
Christine F Carson, Timothy Jj Inglis
This study investigated aerosolized viable bacteria in a university research laboratory during operation of an acoustic-assisted flow cytometer for antimicrobial susceptibility testing by sampling room air before, during and after flow cytometer use. The aim was to assess the risk associated with use of an acoustic-assisted flow cytometer analyzing unfixed bacterial suspensions. Air sampling in a nearby clinical laboratory was conducted during the same period to provide context for the existing background of microorganisms that would be detected in the air...
February 27, 2018: Gates open research
https://www.readbyqxmd.com/read/29579106/impact-of-nutritional-stress-on-drug-susceptibility-and-biofilm-structures-of-burkholderia-pseudomallei-and-burkholderia-thailandensis-grown-in-static-and-microfluidic-systems
#7
Chitchanok Anutrakunchai, Jan G M Bolscher, Bastiaan P Krom, Sakawrat Kanthawong, Sorujsiri Chareonsudjai, Suwimol Taweechaisupapong
Burkholderia pseudomallei is the causative agent of melioidosis and regarded as a bioterrorism threat. It can adapt to the nutrient-limited environment as the bacteria can survive in triple distilled water for 16 years. Moreover, B. pseudomallei exhibits intrinsic resistance to diverse groups of antibiotics in particular while growing in biofilms. Recently, nutrient-limited condition influenced both biofilm formation and ceftazidime (CAZ) tolerance of B. pseudomallei were found. However, there is no information about how nutrient-limitation together with antibiotics used in melioidosis treatment affects the structure of the biofilm produced by B...
2018: PloS One
https://www.readbyqxmd.com/read/29576451/irf8-regulates-transcription-of-naips-for-nlrc4-inflammasome-activation
#8
Rajendra Karki, Ein Lee, David Place, Parimal Samir, Jayadev Mavuluri, Bhesh Raj Sharma, Arjun Balakrishnan, R K Subbarao Malireddi, Rechel Geiger, Qifan Zhu, Geoffrey Neale, Thirumala-Devi Kanneganti
Inflammasome activation is critical for host defenses against various microbial infections. Activation of the NLRC4 inflammasome requires detection of flagellin or type III secretion system (T3SS) components by NLR family apoptosis inhibitory proteins (NAIPs); yet how this pathway is regulated is unknown. Here, we found that interferon regulatory factor 8 (IRF8) is required for optimal activation of the NLRC4 inflammasome in bone-marrow-derived macrophages infected with Salmonella Typhimurium, Burkholderia thailandensis, or Pseudomonas aeruginosa but is dispensable for activation of the canonical and non-canonical NLRP3, AIM2, and Pyrin inflammasomes...
March 16, 2018: Cell
https://www.readbyqxmd.com/read/29507087/-two-rsam-homologues-encode-central-regulatory-elements-modulating-quorum-sensing-in-burkholderia-thailandensis
#9
Servane Le Guillouzer, Marie-Christine Groleau, Eric Déziel
The bacterium Burkholderia thailandensis possesses three N -acyl-L -homoserine lactone (AHL) quorum sensing (QS) systems designated BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). These QS systems are associated with the biosynthesis of N -octanoyl-homoserine lactone (C8 -HSL), N -3-hydroxy-decanoyl-homoserine lactone (3OHC10 -HSL), and N -3-hydroxy-octanoyl-homoserine lactone (3OHC8 -HSL), which are produced by the LuxI-type synthases BtaI1, BtaI2, and BtaI3, and modulated by the LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3...
March 5, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29374267/quorum-sensing-control-of-antibiotic-resistance-stabilizes-cooperation-in-chromobacterium-violaceum
#10
Kara C Evans, Saida Benomar, Lennel A Camuy-Vélez, Ellen B Nasseri, Xiaofei Wang, Benjamin Neuenswander, Josephine R Chandler
Many Proteobacteria use quorum sensing to regulate production of public goods, such as antimicrobials and proteases, that are shared among members of a community. Public goods are vulnerable to exploitation by cheaters, such as quorum sensing-defective mutants. Quorum sensing- regulated private goods, goods that benefit only producing cells, can prevent the emergence of cheaters under certain growth conditions. Previously, we developed a laboratory co-culture model to investigate the importance of quorum-regulated antimicrobials during interspecies competition...
January 26, 2018: ISME Journal
https://www.readbyqxmd.com/read/29364892/presence-of-b-thailandensis-and-b-thailandensis-expressing-b-pseudomallei-like-capsular-polysaccharide-in-thailand-and-their-associations-with-serological-response-to-b-pseudomallei
#11
Viriya Hantrakun, Janjira Thaipadungpanit, Patpong Rongkard, Prapaporn Srilohasin, Premjit Amornchai, Sayan Langla, Mavuto Mukaka, Narisara Chantratita, Vanaporn Wuthiekanun, David A B Dance, Nicholas P J Day, Sharon J Peacock, Direk Limmathurotsakul
BACKGROUND: Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains of which express a B. pseudomallei-like capsular polysaccharide (BTCV), is also commonly found in the environment in Southeast Asia but is considered non-pathogenic. The aim of the study was to determine the distribution of B. thailandensis and its capsular variant in Thailand and investigate whether its presence is associated with a serological response to B...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29353059/ask-family-kinases-are-required-for-optimal-nlrp3-inflammasome-priming
#12
David E Place, Parimal Samir, Rajendra Karki, Benoit Briard, Peter Vogel, Thirumala-Devi Kanneganti
Activation of the multimeric inflammasome complex leads to inflammatory responses to biotic and abiotic triggers. The inflammasome sensor, Nod-like receptor family pyrin domain containing 3 (NLRP3), is activated by a range of stimuli and is tightly regulated to restrict excessive inflammation. Because NLRP3 responds broadly to cellular insults and regulates cell death similar to the stress-activated apoptosis signal-regulating kinases 1 and 2 (ASK1/2), we hypothesized that ASK1/2 may regulate NLRP3 activity...
April 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29320408/outer-membrane-vesicle-vaccines-from-biosafe-surrogates-prevent-acute-lethal-glanders-in-mice
#13
Michael H Norris, Mohammad S R Khan, Sunisa Chirakul, Herbert P Schweizer, Apichai Tuanyok
Burkholderia mallei is a host-adapted Gram-negative mammalian pathogen that causes the severe disease glanders. Glanders can manifest as a rapid acute progression or a chronic debilitating syndrome primarily affecting solipeds and humans in close association with infected animals. In USA, B. mallei is classified as one of the most important bacterial biothreat agents. Presently, there is no licensed glanders vaccine available for humans or animals. In this work, outer membrane vesicles (OMVs) were isolated from three attenuated biosafe bacterial strains, Burkholderia pseudomallei Bp82, B...
January 10, 2018: Vaccines
https://www.readbyqxmd.com/read/29317789/-aspergillus-subgenus-polypaecilum-from-the-built-environment
#14
J B Tanney, C M Visagie, N Yilmaz, K A Seifert
Xerophilic fungi, especially Aspergillus species, are prevalent in the built environment. In this study, we employed a combined culture-independent (454-pyrosequencing) and culture-dependent (dilution-to-extinction) approach to investigate the mycobiota of indoor dust collected from 93 buildings in 12 countries worldwide. High and low water activity (aw ) media were used to capture mesophile and xerophile biodiversity, resulting in the isolation of approximately 9 000 strains. Among these, 340 strains representing seven putative species in Aspergillus subgenus Polypaecilum were isolated, mostly from lowered aw media, and tentatively identified based on colony morphology and internal transcribed spacer rDNA region (ITS) barcodes...
September 2017: Studies in Mycology
https://www.readbyqxmd.com/read/29286449/the-madagascar-hissing-cockroach-as-an-alternative-non-mammalian-animal-model-to-investigate-virulence-pathogenesis-and-drug-efficacy
#15
Jennifer Chua, Nathan A Fisher, Shane D Falcinelli, David DeShazer, Arthur M Friedlander
Many aspects of innate immunity are conserved between mammals and insects. An insect, the Madagascar hissing cockroach from the genus Gromphadorhina, can be utilized as an alternative animal model for the study of virulence, host-pathogen interaction, innate immune response, and drug efficacy. Details for the rearing, care and breeding of the hissing cockroach are provided. We also illustrate how it can be infected with bacteria such as the intracellular pathogens Burkholderia mallei, B. pseudomallei, and B...
November 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29283242/correction-to-global-awakening-of-cryptic-biosynthetic-gene-clusters-in-burkholderia-thailandensis
#16
Ashish Gupta, Renesh Bedre, Sudarshan Singh Thapa, Afsana Sabrin, Guannan Wang, Maheshi Dassanayake, Anne Grove
No abstract text is available yet for this article.
January 19, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29245462/-i-dolichocephala-i-macquart-of-thailand-diptera-empididae-clinocerinae
#17
Bradley J Sinclair, Adrian R Plant
Seven new species of Dolichocephala Macquart from Thailand are described (D. bicolor sp. nov., D. curvata sp. nov., D. immaculata sp. nov., D. incus sp. nov., D. longicerca sp. nov., D. srisukai sp. nov., D. thailandensis sp. nov.). A key to species is provided, distributions mapped and phenology and biogeography discussed.
November 30, 2017: Zootaxa
https://www.readbyqxmd.com/read/29233899/detection-of-cytosolic-shigella-flexneri-via-a-c-terminal-triple-arginine-motif-of-gbp1-inhibits-actin-based-motility
#18
Anthony S Piro, Dulcemaria Hernandez, Sarah Luoma, Eric M Feeley, Ryan Finethy, Azeb Yirga, Eva M Frickel, Cammie F Lesser, Jörn Coers
Dynamin-like guanylate binding proteins (GBPs) are gamma interferon (IFN-γ)-inducible host defense proteins that can associate with cytosol-invading bacterial pathogens. Mouse GBPs promote the lytic destruction of targeted bacteria in the host cell cytosol, but the antimicrobial function of human GBPs and the mechanism by which these proteins associate with cytosolic bacteria are poorly understood. Here, we demonstrate that human GBP1 is unique among the seven human GBP paralogs in its ability to associate with at least two cytosolic Gram-negative bacteria, Burkholderia thailandensis and Shigella flexneri Rough lipopolysaccharide (LPS) mutants of S...
December 12, 2017: MBio
https://www.readbyqxmd.com/read/29208745/the-complex-quorum-sensing-circuitry-of-burkholderia-thailandensis-is-both-hierarchically-and-homeostatically-organized
#19
Servane Le Guillouzer, Marie-Christine Groleau, Eric Déziel
The genome of the bacterium Burkholderia thailandensis encodes three complete LuxI/LuxR-type quorum sensing (QS) systems: BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). The LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3 modulate the expression of target genes in association with various N -acyl-l-homoserine lactones (AHLs) as signaling molecules produced by the LuxI-type synthases BtaI1, BtaI2, and BtaI3. We have systematically dissected the complex QS circuitry of B. thailandensis strain E264...
December 5, 2017: MBio
https://www.readbyqxmd.com/read/29208119/comparative-analysis-of-the-burkholderia-cenocepacia-k56-2-essential-genome-reveals-cell-envelope-functions-that-are-uniquely-required-for-survival-in-species-of-the-genus-burkholderia
#20
April S Gislason, Keith Turner, Mike Domaratzki, Silvia T Cardona
Burkholderia cenocepacia K56-2 belongs to the Burkholderia cepacia complex, a group of Gram-negative opportunistic pathogens that have large and dynamic genomes. In this work, we identified the essential genome of B. cenocepacia K56-2 using high-density transposon mutagenesis and insertion site sequencing (Tn-seq circle). We constructed a library of one million transposon mutants and identified the transposon insertions at an average of one insertion per 27 bp. The probability of gene essentiality was determined by comparing of the insertion density per gene with the variance of neutral datasets generated by Monte Carlo simulations...
November 2017: Microbial Genomics
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