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https://www.readbyqxmd.com/read/28855059/molecular-signature-analyses-support-the-establishment-of-the-actinobacterial-genus-sphaerimonospora-mingma-et-al-2016
#1
Paul R Meyers
The genera Microbispora and Sphaerimonospora were examined for GyrB and RecA amino-acid signatures to determine whether molecular-signature analyses support the recent establishment of the genus Sphaerimonospora. The creation of Sphaerimonospora was based mainly upon morphological differences between Microbispora and Sphaerimonospora and the clustering of the type strains of the two genera in phylogenetic trees based on a multilocus sequence analysis. The molecular-signature analyses showed that all members of Sphaerimonospora can be distinguished from all members of Microbispora at 14 amino acid positions in the GyrB protein and at four positions in the shorter RecA protein...
August 18, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28853680/pseudonocardia-thailandensis-sp-nov-an-actinomycete-isolated-from-a-subterranean-termite-nest
#2
Kanaporn Sujarit, Nikhom Sujada, Takuji Kudo, Moriya Ohkuma, Wasu Pathom-Aree, Saisamorn Lumyong
A novel Gram-stain-positive bacterium designated CMU-NKS-70T was isolated from a subterranean termite nest and characterized using a polyphasic approach. The strain exhibited branching, pinkish-cream aerial mycelium and cream-brown substrate mycelium, and formed chains of rod-like spores. The 16S rRNA gene sequence analyses indicated that strain CMU-NKS-70T belonged to the genus Pseudonocardia, showing high similarity with Pseudonocardia oroxyli D10T (98.9 % 16S rRNA gene sequence similarity), Pseudonocardia xishanensis YIM 63638T (98...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28760929/entry-intracellular-survival-and-multinucleated-giant-cell-forming-activity-of-burkholderia-pseudomallei-in-human-primary-phagocytic-and-non-phagocytic-cells
#3
Liam Whiteley, Teresa Meffert, Maria Haug, Christopher Weidenmaier, Verena Hopf, Katharina Bitschar, Birgit Schittek, Christian Kohler, Ivo Steinmetz, T Eoin West, Sandra Schwarz
The human pathogen Burkholderia pseudomallei and the related species Burkholderia thailandensis are facultative intracellular bacteria characterized by the ability to escape into the cytosol of the host cell and to stimulate the formation of multinucleated giant cells (MNGCs). MNGC formation is induced via an unknown mechanism by the bacterial type VI secretion system 5 (T6SS-5), which is an essential virulence factor in both species. Despite the vital role of the intracellular life cycle in the pathogenesis of the bacteria, the range of host cell types permissive for initiation and completion of the intracellular cycle is poorly defined...
July 31, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28746878/the-type-vi-secretion-system-engages-a-redox-regulated-dual-functional-heme-transporter-for-zinc-acquisition
#4
Meiru Si, Yao Wang, Bing Zhang, Chao Zhao, Yiwen Kang, Haonan Bai, Dawei Wei, Lingfang Zhu, Lei Zhang, Tao G Dong, Xihui Shen
The type VI secretion system was recently reported to be involved in zinc acquisition, but the underlying mechanism remains unclear. Here, we report that Burkholderia thailandensis T6SS4 is involved in zinc acquisition via secretion of a zinc-scavenging protein, TseZ, that interacts with the outer membrane heme transporter HmuR. We find that HmuR is a redox-regulated dual-functional transporter that transports heme iron under normal conditions but zinc upon sensing extracellular oxidative stress, triggered by formation of an intramolecular disulfide bond...
July 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28726626/human-infection-with-burkholderia-thailandensis-china-2013
#5
Kai Chang, Jie Luo, Huan Xu, Min Li, Fengling Zhang, Jin Li, Dayong Gu, Shaoli Deng, Ming Chen, Weiping Lu
Burkholderia thailandensis infection in humans is uncommon. We describe a case of B. thailandensis infection in a person in China, a location heretofore unknown for B. thailandensis. We identified the specific virulence factors of B. thailandensis, which may indicate a transition to a new virulent form.
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28719265/differences-in-inflammation-patterns-induced-by-african-and-asian-burkholderia-pseudomallei-isolates-in-mice
#6
Tassili A F Weehuizen, Emma Birnie, Bart Ferwerda, Joris J T H Roelofs, Alex F de Vos, Martin P Grobusch, W Joost Wiersinga
AbstractBurkholderia pseudomallei is the causative agent of melioidosis, an emerging tropical disease of high mortality. Sub-Saharan Africa represents potential melioidosis "hotspots"; however, to date, only a few cases have been reported. Here in, we compared the inflammatory patterns induced by a B. pseudomallei strain recently isolated from a fatal Gabonese case with the Thai reference strain B. pseudomallei-1026b and Burkholderia thailandensis-E264. Ex vivo, no differences were observed in terms of cellular responsiveness between strains...
June 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28665591/biochemical-characterization-and-structural-basis-of-reactivity-and-regioselectivity-differences-between-burkholderia-thailandensis-and-burkholderia-glumae-1-6-didesmethyltoxoflavin-n-methyltransferase
#7
COMPARATIVE STUDY
Michael K Fenwick, Khaled H Almabruk, Steven E Ealick, Tadhg P Begley, Benjamin Philmus
Burkholderia glumae converts the guanine base of guanosine triphosphate into an azapteridine and methylates both the pyrimidine and triazine rings to make toxoflavin. Strains of Burkholderia thailandensis and Burkholderia pseudomallei have a gene cluster encoding seven putative biosynthetic enzymes that resembles the toxoflavin gene cluster. Four of the enzymes are similar in sequence to BgToxBCDE, which have been proposed to make 1,6-didesmethyltoxoflavin (1,6-DDMT). One of the remaining enzymes, BthII1283 in B...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28638804/pkc-%C3%AE-marcks-signaling-promotes-intracellular-survival-of-unopsonized-burkholderia-thailandensis
#8
Sofiya N Micheva-Viteva, Yulin Shou, Kumkum Ganguly, Terry H Wu, Elizabeth Hong-Geller
Pathogenic Burkholderia rely on host factors for efficient intracellular replication and are highly refractory to antibiotic treatment. To identify host genes that are required by Burkholderia spp. during infection, we performed a RNA interference (RNAi) screen of the human kinome and identified 35 host kinases that facilitated Burkholderia thailandensis intracellular survival in human monocytic THP-1 cells. We validated a selection of host kinases using imaging flow cytometry to assess efficiency of B. thailandensis survival in the host upon siRNA-mediated knockdown...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28634144/development-of-a-multiplex-pcr-assay-for-the-detection-and-differentiation-of-burkholderia-pseudomallei-burkholderia-mallei-burkholderia-thailandensis-and-burkholderia-cepacia-complex
#9
Irina Zakharova, Natalya Teteryatnikova, Andrey Toporkov, Dmitry Viktorov
Two species of Burkholderia pseudomallei complex (Bpc), B. pseudomallei and B. mallei, can cause severe life-threatening infections. Rapidly discerning individual species within the group and separating them from other opportunistic pathogens of the Burkholderia cepacia complex (Bcc) is essential to establish a correct diagnosis and for epidemiological surveillance. In this study, a multiplex PCR assay based on the detection of an individual set of chromosomal beta-lactamase genes for single-step identification and differentiation of B...
October 2017: Acta Tropica
https://www.readbyqxmd.com/read/28624973/gracilibacillus-aidingensis-sp-nov-a-novel-moderately-halophilic-bacterium-isolated-from-aiding-salt-lake
#10
Tong-Wei Guan, Lei Tian, En-Yuan Li, Shu-Kun Tang, Xiao-Ping Zhang
A novel Gram-positive, aerobe, moderately halophilic bacterium was isolated from saline soil of Aiding lake in Xinjiang, north-west of China, designated strain YIM 98001(T). Cells were rod-shaped, motile and grew at 5-20% (w/v) NaCl (optimum 10%), pH 6-10 (optimum pH 7.0) and 4-45 °C (optimum 37 °C). The major cellular fatty acids were anteiso C15:0, anteiso C17:0, iso C15:0. The predominant respiratory quinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid were the major polar lipids...
June 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28510362/burkholderia-thailandensis-genetic-manipulation
#11
Erin C Garcia
Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B...
May 16, 2017: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/28454708/functional-characterisation-of-burkholderia-pseudomallei-biotin-protein-ligase-a-toolkit-for-anti-melioidosis-drug-development
#12
Thomas E H Bond, Alanna E Sorenson, Patrick M Schaeffer
Burkholderia pseudomallei (Bp) is the causative agent of melioidosis. The bacterium is responsible for 20% of community-acquired sepsis cases and 40% of sepsis-related mortalities in northeast Thailand, and is intrinsically resistant to aminoglycosides, macrolides, rifamycins, cephalosporins, and nonureidopenicillins. There is no vaccine and its diagnosis is problematic. Biotin protein ligase (BirA) which is essential for fatty acid synthesis has been proposed as a drug target in bacteria. Very few bacterial BirA have been characterized, and a better understanding of these enzymes is necessary to further assess their value as drug targets...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28406615/redox-sensitive-marr-homologue-bifr-from-burkholderia-thailandensis-regulates-biofilm-formation
#13
Ashish Gupta, Stanley M Fuentes, Anne Grove
Biofilm formation by pathogenic Burkholderia species is a serious complication as it renders the bacteria resistant to antibiotics and host defenses. Using B. thailandensis, we report here a novel redox-sensitive member of the multiple antibiotic resistance regulator (MarR) protein family, BifR, which represses biofilm formation. BifR is encoded as part of the emrB-bifR operon; emrB-bifR is divergent to ecsC, which encodes a putative LasA protease. In Pseudomonas aeruginosa, LasA has been implicated in virulence by contributing to cleavage of elastase...
May 2, 2017: Biochemistry
https://www.readbyqxmd.com/read/28386631/microbial-rhamnolipid-production-a-critical-re-evaluation-of-published-data-and-suggested-future-publication-criteria
#14
REVIEW
Victor U Irorere, Lakshmi Tripathi, Roger Marchant, Stephen McClean, Ibrahim M Banat
High production cost and potential pathogenicity of Pseudomonas aeruginosa, commonly used for rhamnolipid synthesis, have led to extensive research for safer producing strains and cost-effective production methods. This has resulted in numerous research publications claiming new non-pathogenic producing strains and novel production techniques many of which are unfortunately without proper characterisation of product and/or producing strain/s. Genes responsible for rhamnolipid production have only been confirmed in P...
May 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28384252/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-the-identification-of-burkholderia-pseudomallei-from-asia-and-australia-and-differentiation-between-burkholderia-species
#15
Vichaya Suttisunhakul, Apinya Pumpuang, Peeraya Ekchariyawat, Vanaporn Wuthiekanun, Mindy G Elrod, Paul Turner, Bart J Currie, Rattanaphone Phetsouvanh, David A B Dance, Direk Limmathurotsakul, Sharon J Peacock, Narisara Chantratita
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28369538/structure-of-acid-deoxyribonuclease
#16
Armando Varela-Ramirez, Jan Abendroth, Adrian A Mejia, Isabelle Q Phan, Donald D Lorimer, Thomas E Edwards, Renato J Aguilera
Deoxyribonuclease II (DNase II) is also known as acid deoxyribonuclease because it has optimal activity at the low pH environment of lysosomes where it is typically found in higher eukaryotes. Interestingly, DNase II has also been identified in a few genera of bacteria and is believed to have arisen via horizontal transfer. Here, we demonstrate that recombinant Burkholderia thailandensis DNase II is highly active at low pH in the absence of divalent metal ions, similar to eukaryotic DNase II. The crystal structure of B...
June 2, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28320949/discovery-of-scmr-as-a-global-regulator-of-secondary-metabolism-and-virulence-in-burkholderia-thailandensis-e264
#17
Dainan Mao, Leah B Bushin, Kyuho Moon, Yihan Wu, Mohammad R Seyedsayamdost
Bacteria produce a diverse array of secondary metabolites that have been invaluable in the clinic and in research. These metabolites are synthesized by dedicated biosynthetic gene clusters (BGCs), which assemble architecturally complex molecules from simple building blocks. The majority of BGCs in a given bacterium are not expressed under normal laboratory growth conditions, and our understanding of how they are silenced is in its infancy. Here, we have addressed this question in the Gram-negative model bacterium Burkholderia thailandensis E264 using genetic, transcriptomic, metabolomic, and chemical approaches...
April 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28303005/ligand-co-crystallization-of-aminoacyl-trna-synthetases-from-infectious-disease-organisms
#18
Spencer O Moen, Thomas E Edwards, David M Dranow, Matthew C Clifton, Banumathi Sankaran, Wesley C Van Voorhis, Amit Sharma, Colin Manoil, Bart L Staker, Peter J Myler, Donald D Lorimer
Aminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28242693/manganese-scavenging-and-oxidative-stress-response-mediated-by-type-vi-secretion-system-in-burkholderia-thailandensis
#19
Meiru Si, Chao Zhao, Brianne Burkinshaw, Bing Zhang, Dawei Wei, Yao Wang, Tao G Dong, Xihui Shen
Type VI secretion system (T6SS) is a versatile protein export machinery widely distributed in Gram-negative bacteria. Known to translocate protein substrates to eukaryotic and prokaryotic target cells to cause cellular damage, the T6SS has been primarily recognized as a contact-dependent bacterial weapon for microbe-host and microbial interspecies competition. Here we report contact-independent functions of the T6SS for metal acquisition, bacteria competition, and resistance to oxidative stress. We demonstrate that the T6SS-4 in Burkholderia thailandensis is critical for survival under oxidative stress and is regulated by OxyR, a conserved oxidative stress regulator...
March 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28186697/complement-activated-vitronectin-enhances-the-invasion-of-nonphagocytic-cells-by-bacterial-pathogens-burkholderia-and-klebsiella
#20
Yi Han Tan, Akshamal M Gamage, Yunn-Hwen Gan
Burkholderia pseudomallei is a serum-resistant Gram-negative bacterium capable of causing disseminated infections with metastatic complications. However, its interaction with nonphagocytic cells is poorly understood. We observed that exposure of B. pseudomallei and the closely related yet avirulent B. thailandensis to human plasma increased epithelial cell invasion by >20 fold. Enhanced invasion was primarily driven by a plasma factor, which required a functional complement cascade, but surprisingly, was downstream of C3 mediated opsonisation...
February 10, 2017: Cellular Microbiology
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