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https://www.readbyqxmd.com/read/29136176/mutations-in-metg-methionyl-trna-synthetase-and-trmd-trna-guanine-n1-methyltransferase-conferring-meropenem-tolerance-in-burkholderia-thailandensis
#1
Hyojeong Yi, Hyeri Lee, Kwang-Hwi Cho, Heenam Stanley Kim
Objectives: Although meropenem is widely used to treat Burkholderia infections, the response of Burkholderia pathogens to this antibiotic is largely unexplored. Methods: Burkholderia thailandensis, a model for Burkholderia spp., particularly Burkholderia mallei and Burkholderia pseudomallei, was challenged with a lethal level of meropenem and survivors were isolated. The genomes of two of the isolates were analysed to identify mutated genes and these genes were then specifically examined in more isolates to profile mutation diversity...
November 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29122686/an-artificially-constructed-dimer-through-deformation-of-a-short-zinc-binding-loop
#2
Jimin Park, Mi-Sun Kim, Keehyoung Joo, Jooyoung Lee, Dong Hae Shin
We have analyzed the crystal structure of the dimeric form of d-glycero-d-manno-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis (BtGmhB), catalyzing the removal of the phosphate at the 7 position of d-glycero-d-manno-heptose-1,7-bisphosphate. The crystal structure of BtGmhB revealed a dimeric form caused by a disruption of a short zinc-binding loop. The dimeric BtGmhB structure was induced by triggering the loss of Zn(2+)via the protonation of cysteine residues at pH 4.8 of the crystallization condition...
November 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29089426/synergy-between-active-efflux-and-outer-membrane-diffusion-defines-rules-of-antibiotic-permeation-into-gram-negative-bacteria
#3
Ganesh Krishnamoorthy, Inga V Leus, Jon W Weeks, David Wolloscheck, Valentin V Rybenkov, Helen I Zgurskaya
Gram-negative bacteria are notoriously resistant to antibiotics, but the extent of the resistance varies broadly between species. We report that in significant human pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Burkholderia spp., the differences in antibiotic resistance are largely defined by their penetration into the cell. For all tested antibiotics, the intracellular penetration was determined by a synergistic relationship between active efflux and the permeability barrier. We found that the outer membrane (OM) and efflux pumps select compounds on the basis of distinct properties and together universally protect bacteria from structurally diverse antibiotics...
October 31, 2017: MBio
https://www.readbyqxmd.com/read/29050323/histone-deacetylase-inhibitor-thailandepsin-a-activates-notch-signaling-and-suppresses-neuroendocrine-cancer-cell-growth-in-vivo
#4
Samuel Jang, Andrew Janssen, Zviadi Aburjania, Matthew B Robers, April Harrison, Ajitha Dammalapati, Yi-Qiang Cheng, Herbert Chen, Renata Jaskula-Sztul
Novel therapies for neuroendocrine (NE) cancers are desperately needed as they frequently present as metastatic disease and cause debilitating symptoms by secreting excessive hormones. Induction of Notch isoforms has a tumor suppressive effect in NE cancer cell lines, and we have observed that histone deacetylase inhibitors (HDACi) potently activate Notch. In this study, we describe the potential for Burkholderia thailandensis-derived class I HDACi thailandepsin A (TDP-A) as a Notch activator and therapeutic agent against NE cancer...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29043376/enhanced-rhamnolipid-production-in-burkholderia-thailandensis-transposon-knockout-strains-deficient-in-polyhydroxyalkanoate-pha-synthesis
#5
Scott J Funston, Konstantina Tsaousi, Thomas J Smyth, Matthew S Twigg, Roger Marchant, Ibrahim M Banat
Microbially produced rhamnolipids have significant commercial potential; however, the main bacterial producer, Pseudomonas aeruginosa, is an opportunistic human pathogen, which limits biotechnological exploitation. The non-pathogenic species Burkholderia thailandensis produces rhamnolipids; however, yield is relatively low. The aim of this study was to determine whether rhamnolipid production could be increased in Burkholderia thailandensis through mutation of genes responsible for the synthesis of the storage material polyhydroxyalkanoate (PHA), thereby increasing cellular resources for the production of rhamnolipids...
December 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29033316/synergy-and-target-promiscuity-drive-structural-divergence-in-bacterial-alkylquinolone-biosynthesis
#6
Yihan Wu, Mohammad R Seyedsayamdost
Microbial natural products are genetically encoded by dedicated biosynthetic gene clusters (BGCs). A given BGC usually produces a family of related compounds that share a core but contain variable substituents. Though common, the reasons underlying this divergent biosynthesis are in general unknown. Herein, we have addressed this issue using the hydroxyalkylquinoline (HAQ) family of natural products synthesized by Burkholderia thailandensis. Investigations into the detailed functions of two analogs show that they act synergistically in inhibiting bacterial growth...
September 28, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/29024844/structural-characterisation-of-the-capsular-polysaccharide-expressed-by-burkholderia-thailandensis-strain-e555-wbii-pknock-kmr-and-assessment-of-the-significance-of-the-2-o-acetyl-group-in-immune-protection
#7
Marc Bayliss, Matthew I Donaldson, Sergey A Nepogodiev, Giulia Pergolizzi, Andrew E Scott, Nicholas J Harmer, Robert A Field, Joann L Prior
Burkholderia pseudomallei and its close relative B. mallei are human pathogens that are classified as Tier 1 bio-threat agents. Both organisms have previously been shown to constitutively produce a capsular polysaccharide (CPS) that is both a virulence determinant and protective antigen. Extraction and purification of CPS for use as a potential vaccine candidate requires containment level 3 laboratories which is expensive and time-consuming. B. thailandensis strain E555 is closely related to B. pseudomallei and B...
November 27, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28973030/cholesterol-and-host-cell-surface-proteins-contribute-to-cell-cell-fusion-induced-by-the-burkholderia-type-vi-secretion-system-5
#8
Liam Whiteley, Maria Haug, Kristina Klein, Matthias Willmann, Erwin Bohn, Salvatore Chiantia, Sandra Schwarz
Following escape into the cytoplasm of host cells, Burkholderia pseudomallei and the related species Burkholderia thailandensis employ the type VI secretion system 5 (T6SS-5) to induce plasma membrane fusion with an adjacent host cell. This process leads to the formation of multinucleated giant cells and facilitates bacterial access to an uninfected host cell in a direct manner. Despite its importance in virulence, the mechanism of the T6SS-5 and the role of host cell factors in cell-cell fusion remain elusive...
2017: PloS One
https://www.readbyqxmd.com/read/28965656/effects-of-binge-alcohol-exposure-on-burkholderia-thailandensis-alveolar-macrophage-interaction
#9
Victor Jimenez, Ryan Moreno, Emily Kaufman, Heidie Hornstra, Erik Settles, Bart J Currie, Paul Keim, Fernando P Monroy
Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. One example is melioidosis, a disease caused by infection with Burkholderia pseudomallei, which is of public health importance in Southeast Asia and Northern Australia, with an expanding global distribution. While B. pseudomallei infections can occur in healthy humans, binge alcohol use is progressively being recognized as a major risk factor. Although binge alcohol consumption has been considered as a risk factor for the development of melioidosis, no experimental studies have investigated the outcomes of alcohol exposure on Burkholderia spp...
November 2017: Alcohol
https://www.readbyqxmd.com/read/28855059/molecular-signature-analyses-support-the-establishment-of-the-actinobacterial-genus-sphaerimonospora-mingma-et-al-2016
#10
Paul R Meyers
The genera Microbispora and Sphaerimonospora were examined for GyrB and RecA amino-acid signatures to determine whether molecular-signature analyses support the recent establishment of the genus Sphaerimonospora. The creation of Sphaerimonospora was based mainly upon morphological differences between Microbispora and Sphaerimonospora and the clustering of the type strains of the two genera in phylogenetic trees based on a multilocus sequence analysis. The molecular-signature analyses showed that all members of Sphaerimonospora can be distinguished from all members of Microbispora at 14 amino acid positions in the GyrB protein and at four positions in the shorter RecA protein...
August 18, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28853680/pseudonocardia-thailandensis-sp-nov-an-actinomycete-isolated-from-a-subterranean-termite-nest
#11
Kanaporn Sujarit, Nikhom Sujada, Takuji Kudo, Moriya Ohkuma, Wasu Pathom-Aree, Saisamorn Lumyong
A novel Gram-stain-positive bacterium designated CMU-NKS-70T was isolated from a subterranean termite nest and characterized using a polyphasic approach. The strain exhibited branching, pinkish-cream aerial mycelium and cream-brown substrate mycelium, and formed chains of rod-like spores. The 16S rRNA gene sequence analyses indicated that strain CMU-NKS-70T belonged to the genus Pseudonocardia, showing high similarity with Pseudonocardia oroxyli D10T (98.9 % 16S rRNA gene sequence similarity), Pseudonocardia xishanensis YIM 63638T (98...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28760929/entry-intracellular-survival-and-multinucleated-giant-cell-forming-activity-of-burkholderia-pseudomallei-in-human-primary-phagocytic-and-nonphagocytic-cells
#12
Liam Whiteley, Teresa Meffert, Maria Haug, Christopher Weidenmaier, Verena Hopf, Katharina Bitschar, Birgit Schittek, Christian Kohler, Ivo Steinmetz, T Eoin West, Sandra Schwarz
The human pathogen Burkholderia pseudomallei and the related species Burkholderia thailandensis are facultative intracellular bacteria characterized by the ability to escape into the cytosol of the host cell and to stimulate the formation of multinucleated giant cells (MNGCs). MNGC formation is induced via an unknown mechanism by bacterial type VI secretion system 5 (T6SS-5), which is an essential virulence factor in both species. Despite the vital role of the intracellular life cycle in the pathogenesis of the bacteria, the range of host cell types permissive for initiation and completion of the intracellular cycle is poorly defined...
October 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28746878/the-type-vi-secretion-system-engages-a-redox-regulated-dual-functional-heme-transporter-for-zinc-acquisition
#13
Meiru Si, Yao Wang, Bing Zhang, Chao Zhao, Yiwen Kang, Haonan Bai, Dawei Wei, Lingfang Zhu, Lei Zhang, Tao G Dong, Xihui Shen
The type VI secretion system was recently reported to be involved in zinc acquisition, but the underlying mechanism remains unclear. Here, we report that Burkholderia thailandensis T6SS4 is involved in zinc acquisition via secretion of a zinc-scavenging protein, TseZ, that interacts with the outer membrane heme transporter HmuR. We find that HmuR is a redox-regulated dual-functional transporter that transports heme iron under normal conditions but zinc upon sensing extracellular oxidative stress, triggered by formation of an intramolecular disulfide bond...
July 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28726626/human-infection-with-burkholderia-thailandensis-china-2013
#14
Kai Chang, Jie Luo, Huan Xu, Min Li, Fengling Zhang, Jin Li, Dayong Gu, Shaoli Deng, Ming Chen, Weiping Lu
Burkholderia thailandensis infection in humans is uncommon. We describe a case of B. thailandensis infection in a person in China, a location heretofore unknown for B. thailandensis. We identified the specific virulence factors of B. thailandensis, which may indicate a transition to a new virulent form.
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28719265/differences-in-inflammation-patterns-induced-by-african-and-asian-burkholderia-pseudomallei-isolates-in-mice
#15
Tassili A F Weehuizen, Emma Birnie, Bart Ferwerda, Joris J T H Roelofs, Alex F de Vos, Martin P Grobusch, W Joost Wiersinga
AbstractBurkholderia pseudomallei is the causative agent of melioidosis, an emerging tropical disease of high mortality. Sub-Saharan Africa represents potential melioidosis "hotspots"; however, to date, only a few cases have been reported. Here in, we compared the inflammatory patterns induced by a B. pseudomallei strain recently isolated from a fatal Gabonese case with the Thai reference strain B. pseudomallei-1026b and Burkholderia thailandensis-E264. Ex vivo, no differences were observed in terms of cellular responsiveness between strains...
June 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28665591/biochemical-characterization-and-structural-basis-of-reactivity-and-regioselectivity-differences-between-burkholderia-thailandensis-and-burkholderia-glumae-1-6-didesmethyltoxoflavin-n-methyltransferase
#16
COMPARATIVE STUDY
Michael K Fenwick, Khaled H Almabruk, Steven E Ealick, Tadhg P Begley, Benjamin Philmus
Burkholderia glumae converts the guanine base of guanosine triphosphate into an azapteridine and methylates both the pyrimidine and triazine rings to make toxoflavin. Strains of Burkholderia thailandensis and Burkholderia pseudomallei have a gene cluster encoding seven putative biosynthetic enzymes that resembles the toxoflavin gene cluster. Four of the enzymes are similar in sequence to BgToxBCDE, which have been proposed to make 1,6-didesmethyltoxoflavin (1,6-DDMT). One of the remaining enzymes, BthII1283 in B...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28638804/pkc-%C3%AE-marcks-signaling-promotes-intracellular-survival-of-unopsonized-burkholderia-thailandensis
#17
Sofiya N Micheva-Viteva, Yulin Shou, Kumkum Ganguly, Terry H Wu, Elizabeth Hong-Geller
Pathogenic Burkholderia rely on host factors for efficient intracellular replication and are highly refractory to antibiotic treatment. To identify host genes that are required by Burkholderia spp. during infection, we performed a RNA interference (RNAi) screen of the human kinome and identified 35 host kinases that facilitated Burkholderia thailandensis intracellular survival in human monocytic THP-1 cells. We validated a selection of host kinases using imaging flow cytometry to assess efficiency of B. thailandensis survival in the host upon siRNA-mediated knockdown...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28634144/development-of-a-multiplex-pcr-assay-for-the-detection-and-differentiation-of-burkholderia-pseudomallei-burkholderia-mallei-burkholderia-thailandensis-and-burkholderia-cepacia-complex
#18
Irina Zakharova, Natalya Teteryatnikova, Andrey Toporkov, Dmitry Viktorov
Two species of Burkholderia pseudomallei complex (Bpc), B. pseudomallei and B. mallei, can cause severe life-threatening infections. Rapidly discerning individual species within the group and separating them from other opportunistic pathogens of the Burkholderia cepacia complex (Bcc) is essential to establish a correct diagnosis and for epidemiological surveillance. In this study, a multiplex PCR assay based on the detection of an individual set of chromosomal beta-lactamase genes for single-step identification and differentiation of B...
October 2017: Acta Tropica
https://www.readbyqxmd.com/read/28624973/gracilibacillus-aidingensis-sp-nov-a-novel-moderately-halophilic-bacterium-isolated-from-aiding-salt-lake
#19
Tong-Wei Guan, Lei Tian, En-Yuan Li, Shu-Kun Tang, Xiao-Ping Zhang
A novel Gram-positive, aerobe, moderately halophilic bacterium was isolated from saline soil of Aiding lake in Xinjiang, north-west of China, designated strain YIM 98001(T). Cells were rod-shaped, motile and grew at 5-20% (w/v) NaCl (optimum 10%), pH 6-10 (optimum pH 7.0) and 4-45 °C (optimum 37 °C). The major cellular fatty acids were anteiso C15:0, anteiso C17:0, iso C15:0. The predominant respiratory quinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid were the major polar lipids...
June 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28510362/burkholderia-thailandensis-genetic-manipulation
#20
Erin C Garcia
Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B...
May 16, 2017: Current Protocols in Microbiology
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