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https://www.readbyqxmd.com/read/28634144/development-of-a-multiplex-pcr-assay-for-the-detection-and-differentiation-of-burkholderia-pseudomallei-burkholderia-mallei-burkholderia-thailandensis-and-burkholderia-cepacia-complex
#1
Irina Zakharova, Natalya Teteryatnikova, Andrey Toporkov, Dmitry Viktorov
Two species of Burkholderia pseudomallei complex (Bpc), B. pseudomallei and B. mallei, can cause severe life-threatening infections. Rapidly discerning individual species within the group and separating them from other opportunistic pathogens of the Burkholderia cepacia complex (Bcc) is essential to establish a correct diagnosis and for epidemiological surveillance. In this study, a multiplex PCR assay based on the detection of an individual set of chromosomal beta-lactamase genes for single-step identification and differentiation of B...
June 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28624973/gracilibacillus-aidingensis-sp-nov-a-novel-moderately-halophilic-bacterium-isolated-from-aiding-salt-lake
#2
Tong-Wei Guan, Lei Tian, En-Yuan Li, Shu-Kun Tang, Xiao-Ping Zhang
A novel Gram-positive, aerobe, moderately halophilic bacterium was isolated from saline soil of Aiding lake in Xinjiang, north-west of China, designated strain YIM 98001(T). Cells were rod-shaped, motile and grew at 5-20% (w/v) NaCl (optimum 10%), pH 6-10 (optimum pH 7.0) and 4-45 °C (optimum 37 °C). The major cellular fatty acids were anteiso C15:0, anteiso C17:0, iso C15:0. The predominant respiratory quinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid were the major polar lipids...
June 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28510362/burkholderia-thailandensis-genetic-manipulation
#3
Erin C Garcia
Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B...
May 16, 2017: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/28454708/functional-characterisation-of-burkholderia-pseudomallei-biotin-protein-ligase-a-toolkit-for-anti-melioidosis-drug-development
#4
Thomas E H Bond, Alanna E Sorenson, Patrick M Schaeffer
Burkholderia pseudomallei (Bp) is the causative agent of melioidosis. The bacterium is responsible for 20% of community-acquired sepsis cases and 40% of sepsis-related mortalities in northeast Thailand, and is intrinsically resistant to aminoglycosides, macrolides, rifamycins, cephalosporins, and nonureidopenicillins. There is no vaccine and its diagnosis is problematic. Biotin protein ligase (BirA) which is essential for fatty acid synthesis has been proposed as a drug target in bacteria. Very few bacterial BirA have been characterized, and a better understanding of these enzymes is necessary to further assess their value as drug targets...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28406615/redox-sensitive-marr-homologue-bifr-from-burkholderia-thailandensis-regulates-biofilm-formation
#5
Ashish Gupta, Stanley M Fuentes, Anne Grove
Biofilm formation by pathogenic Burkholderia species is a serious complication as it renders the bacteria resistant to antibiotics and host defenses. Using B. thailandensis, we report here a novel redox-sensitive member of the multiple antibiotic resistance regulator (MarR) protein family, BifR, which represses biofilm formation. BifR is encoded as part of the emrB-bifR operon; emrB-bifR is divergent to ecsC, which encodes a putative LasA protease. In Pseudomonas aeruginosa, LasA has been implicated in virulence by contributing to cleavage of elastase...
April 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28386631/microbial-rhamnolipid-production-a-critical-re-evaluation-of-published-data-and-suggested-future-publication-criteria
#6
REVIEW
Victor U Irorere, Lakshmi Tripathi, Roger Marchant, Stephen McClean, Ibrahim M Banat
High production cost and potential pathogenicity of Pseudomonas aeruginosa, commonly used for rhamnolipid synthesis, have led to extensive research for safer producing strains and cost-effective production methods. This has resulted in numerous research publications claiming new non-pathogenic producing strains and novel production techniques many of which are unfortunately without proper characterisation of product and/or producing strain/s. Genes responsible for rhamnolipid production have only been confirmed in P...
May 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28384252/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-the-identification-of-burkholderia-pseudomallei-from-asia-and-australia-and-differentiation-between-burkholderia-species
#7
Vichaya Suttisunhakul, Apinya Pumpuang, Peeraya Ekchariyawat, Vanaporn Wuthiekanun, Mindy G Elrod, Paul Turner, Bart J Currie, Rattanaphone Phetsouvanh, David A B Dance, Direk Limmathurotsakul, Sharon J Peacock, Narisara Chantratita
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28369538/structure-of-acid-deoxyribonuclease
#8
Armando Varela-Ramirez, Jan Abendroth, Adrian A Mejia, Isabelle Q Phan, Donald D Lorimer, Thomas E Edwards, Renato J Aguilera
Deoxyribonuclease II (DNase II) is also known as acid deoxyribonuclease because it has optimal activity at the low pH environment of lysosomes where it is typically found in higher eukaryotes. Interestingly, DNase II has also been identified in a few genera of bacteria and is believed to have arisen via horizontal transfer. Here, we demonstrate that recombinant Burkholderia thailandensis DNase II is highly active at low pH in the absence of divalent metal ions, similar to eukaryotic DNase II. The crystal structure of B...
June 2, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28320949/discovery-of-scmr-as-a-global-regulator-of-secondary-metabolism-and-virulence-in-burkholderia-thailandensis-e264
#9
Dainan Mao, Leah B Bushin, Kyuho Moon, Yihan Wu, Mohammad R Seyedsayamdost
Bacteria produce a diverse array of secondary metabolites that have been invaluable in the clinic and in research. These metabolites are synthesized by dedicated biosynthetic gene clusters (BGCs), which assemble architecturally complex molecules from simple building blocks. The majority of BGCs in a given bacterium are not expressed under normal laboratory growth conditions, and our understanding of how they are silenced is in its infancy. Here, we have addressed this question in the Gram-negative model bacterium Burkholderia thailandensis E264 using genetic, transcriptomic, metabolomic, and chemical approaches...
April 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28303005/ligand-co-crystallization-of-aminoacyl-trna-synthetases-from-infectious-disease-organisms
#10
Spencer O Moen, Thomas E Edwards, David M Dranow, Matthew C Clifton, Banumathi Sankaran, Wesley C Van Voorhis, Amit Sharma, Colin Manoil, Bart L Staker, Peter J Myler, Donald D Lorimer
Aminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28242693/manganese-scavenging-and-oxidative-stress-response-mediated-by-type-vi-secretion-system-in-burkholderia-thailandensis
#11
Meiru Si, Chao Zhao, Brianne Burkinshaw, Bing Zhang, Dawei Wei, Yao Wang, Tao G Dong, Xihui Shen
Type VI secretion system (T6SS) is a versatile protein export machinery widely distributed in Gram-negative bacteria. Known to translocate protein substrates to eukaryotic and prokaryotic target cells to cause cellular damage, the T6SS has been primarily recognized as a contact-dependent bacterial weapon for microbe-host and microbial interspecies competition. Here we report contact-independent functions of the T6SS for metal acquisition, bacteria competition, and resistance to oxidative stress. We demonstrate that the T6SS-4 in Burkholderia thailandensis is critical for survival under oxidative stress and is regulated by OxyR, a conserved oxidative stress regulator...
March 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28186697/complement-activated-vitronectin-enhances-the-invasion-of-nonphagocytic-cells-by-bacterial-pathogens-burkholderia-and-klebsiella
#12
Yi Han Tan, Akshamal M Gamage, Yunn-Hwen Gan
Burkholderia pseudomallei is a serum-resistant Gram-negative bacterium capable of causing disseminated infections with metastatic complications. However, its interaction with nonphagocytic cells is poorly understood. We observed that exposure of B. pseudomallei and the closely related yet avirulent B. thailandensis to human plasma increased epithelial cell invasion by >20 fold. Enhanced invasion was primarily driven by a plasma factor, which required a functional complement cascade, but surprisingly, was downstream of C3 mediated opsonisation...
February 10, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#13
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28050857/secondary-metabolites-from-bacillus-amyloliquefaciens-isolated-from-soil-can-kill-burkholderia-pseudomallei
#14
Patcharaporn Boottanun, Chotima Potisap, Julian G Hurdle, Rasana W Sermswan
Bacillus species are Gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. In this study, Bacillus amyloliquefaciens N2-4 and N3-8 were isolated from soil and their metabolites could kill Burkholderia pseudomallei, a Gram-negative pathogenic bacterium also found in soil in its endemic areas. Moreover, the metabolites were able to kill drug resistant isolates of B. pseudomallei and also inhibit other pathogenic bacteria such as Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii but not the non-pathogenic Burkholderia thailandensis, which is closely related to B...
December 2017: AMB Express
https://www.readbyqxmd.com/read/27986727/burkholderia-humptydooensis-sp-nov-a-new-species-related-to-burkholderia-thailandensis-and-the-fifth-member-of-the-burkholderia-pseudomallei-complex
#15
Apichai Tuanyok, Mark Mayo, Holger Scholz, Carina M Hall, Christopher J Allender, Mirjam Kaestli, Jennifer Ginther, Senanu Spring-Pearson, Molly C Bollig, Joshua K Stone, Erik W Settles, Joseph D Busch, Lindsay Sidak-Loftis, Jason W Sahl, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Jay E Gee, Richard A Bowen, Jason T Ladner, Sean Lovett, Galina Koroleva, Gustavo Palacios, David M Wagner, Bart J Currie, Paul Keim
During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43(T), MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses...
March 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27936381/a-proteomic-approach-for-rapid-identification-of-weissella-species-isolated-from-korean-fermented-foods-on-maldi-tof-ms-supplemented-with-an-in-house-database
#16
Eiseul Kim, Youngjae Cho, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Weissella are obligate heterofermentative lactic acid bacteria belonging to the Leuconostocaceae family. Some Weissella can be found in salted and fermented foods, such as kimchi and jeotgal, and plays an important role in the fermentation process. In the present study, for the first time, a rapid and accurate identification method for Weissella species from kimchi and jeotgal was developed based on MALDI-TOF MS, supplemented with an in-house database. Of the 135 Weissella spectra aligned with the MALDI bioTyper database, 56 isolates (41...
February 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27894256/burkholderia-pseudomallei-derived-mir-3473-enhances-nf-%C3%AE%C2%BAb-via-targeting-traf3-and-is-associated-with-different-inflammatory-responses-compared-to-burkholderia-thailandensis-in-murine-macrophages
#17
Yao Fang, Hai Chen, Yi Hu, Qian Li, Zhiqiang Hu, Tengfei Ma, Xuhu Mao
BACKGROUND: Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a kind of tropical disease. Burkholderia thailandensis (Bt), with a high sequence similarity to Bp, is thought to be an avirulent organism. Since there are numerous similarities between Bp and Bt, their differences in pathogenesis of host response and related mechanism are still undermined. In recent years, microRNAs have been researched in many diseases, but seldom involved in bacterial infection, bacteria-host interaction or explaining the differences between virulent and avirulent species...
November 28, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27862204/a-comparison-of-the-immunological-potency-of-burkholderia-lipopolysaccharides-in-endotoxemic-balb-c-mice
#18
Pei-Tan Hsueh, Chiu-Lin Liu, Hsuan-Han Wang, Wei-Fen Ni, Ya-Lei Chen, Jong-Kang Liu
Lipopolysaccharide is one of the virulence factors of the soil-borne pathogens Burkholderia pseudomallei, B. thailandensis, B. cenocepacia and B. multivorans, which cause septic melioidosis (often in B. pseudomallei infections but rarely in B. thailandensis infections) or cepacia syndromes (commonly in B. cenocepacia infections but rarely in B. multivorans infections). The inflammatory responses in Burkholderia LPS-induced endotoxemia were evaluated in this study. Prior to induction, the conserved structures and functions of each purified LPS were determined using electrophoretic phenotypes, the ratios of 3-hydroxytetradecanoic to 3-hydroxyhexadecanoic acid and endotoxin units...
November 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27825973/an-efficient-system-for-the-generation-of-marked-genetic-mutants-in-members-of-the-genus-burkholderia
#19
Sravanthi Shastri, Helena L Spiewak, Aderonke Sofoluwe, Vigdis A Eidsvaag, Atif H Asghar, Tyrone Pereira, Edward H Bull, Aaron T Butt, Mark S Thomas
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc)...
January 2017: Plasmid
https://www.readbyqxmd.com/read/27812452/efficacy-of-indirect-elisa-for-serodiagnosis-of-melioidosis-using-immunodominant-antigens-from-non-pathogenic-burkholderia-thailandensis
#20
Sumet Wajanarogana, Kanyanan Kritsiriwuthinan
Melioidosis caused by gram negative bacteria, B. pseudomallei, is a fatal disease in the tropical and sub-tropical regions. However, sporadic cases have been reported in elsewhere. Early detection is imperative to reduce the mortality rate. Serological tests have being substantially developed using recombinant proteins as specific targeted antigens to melioidosis antibodies. In the present study, we focus on a truncated flagellin fragment (FLAG300) and outer membrane protein A (OmpABT) of B. thailandensis E264 as potential antigens for developing indirect ELISA to improve the serodiagnosis of melioidosis...
2016: SpringerPlus
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