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https://www.readbyqxmd.com/read/29049942/bioaugmentation-of-thiabendazole-contaminated-soils-from-a-wastewater-disposal-site-factors-driving-the-efficacy-of-this-strategy-and-the-diversity-of-the-indigenous-soil-bacterial-community
#1
Evangelia S Papadopoulou, Savvas Genitsaris, Michalis Omirou, Chiara Perruchon, Anastasia Stamatopoulou, Ioannis Ioannides, Dimitrios G Karpouzas
The application of the fungicide thiabendazole (TBZ) in fruit packaging plants (FPP) results in the production of effluents which are often disposed in adjacent field sites. These require remediation to prevent further environmental dispersal of TBZ. We assessed the bioaugmentation potential of a newly isolated TBZ-degrading bacterial consortium in a naturally contaminated soil (NCS) exhibiting a natural gradient of TBZ levels (12000, 400, 250 and 12 mg kg(-1)). The effect of aging on bioaugmentation efficacy was comparatively tested in a soil with similar physicochemical properties and soil microbiota, which was artificially, contaminated with the same TBZ levels (ACS)...
October 16, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29049798/near-infrared-fluorescence-guided-thoracoscopic-surgical-intervention-for-postoperative-chylothorax
#2
Feng Yang, Jian Zhou, Hao Li, Fan Yang, Rongxin Xiao, Chongwei Chi, Jie Tian, Jun Wang
OBJECTIVES: Chylothorax is an infrequent but relatively serious complication after lung cancer surgery. Finding the leakage site and identifying the thoracic duct (TD) are the key points of surgical intervention for chylothorax. In this study, for the first time, we demonstrated near-infrared fluorescence imaging with indocyanine green (ICG) in video-assisted thoracoscopic surgery (VATS) intervention for chylothorax in humans. METHODS: This study included 4 patients diagnosed with chylothorax after lung cancer surgery who underwent VATS intervention; 0...
September 25, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049373/barcoding-the-butterflies-of-southern-south-america-species-delimitation-efficacy-cryptic-diversity-and-geographic-patterns-of-divergence
#3
Pablo D Lavinia, Ezequiel O Núñez Bustos, Cecilia Kopuchian, Darío A Lijtmaer, Natalia C García, Paul D N Hebert, Pablo L Tubaro
Because the tropical regions of America harbor the highest concentration of butterfly species, its fauna has attracted considerable attention. Much less is known about the butterflies of southern South America, particularly Argentina, where over 1,200 species occur. To advance understanding of this fauna, we assembled a DNA barcode reference library for 417 butterfly species of Argentina, focusing on the Atlantic Forest, a biodiversity hotspot. We tested the efficacy of this library for specimen identification, used it to assess the frequency of cryptic species, and examined geographic patterns of genetic variation, making this study the first large-scale genetic assessment of the butterflies of southern South America...
2017: PloS One
https://www.readbyqxmd.com/read/29047910/fast-lithographic-source-optimization-using-a-batch-processing-sequential-least-square-estimator
#4
Xu Ma, Haijun Lin, Guoli Jiao, Yanqiu Li, Gonzalo R Arce
This paper proposes a fast source optimization (SO) method for lithography systems to improve the imaging performance of different hotspots on the fullchip layout. Hotspots are referred to as the critical locations on the layout that are difficult to print. A fullchip layout usually includes numerous hotspots with different geometric characteristics. Current SO approaches collect all of the data from different hotspots before the optimization, and then try to calculate the common optimal source for all hotspots...
July 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/29046685/comparative-analysis-of-the-complete-plastomes-of-apostasia-wallichii-and-neuwiedia-singapureana-apostasioideae-reveals-different-evolutionary-dynamics-of-ir-ssc-boundary-among-photosynthetic-orchids
#5
Zhitao Niu, Jiajia Pan, Shuying Zhu, Ludan Li, Qingyun Xue, Wei Liu, Xiaoyu Ding
Apostasioideae, consists of only two genera, Apostasia and Neuwiedia, which are mainly distributed in Southeast Asia and northern Australia. The floral structure, taxonomy, biogeography, and genome variation of Apostasioideae have been intensively studied. However, detailed analyses of plastome composition and structure and comparisons with those of other orchid subfamilies have not yet been conducted. Here, the complete plastome sequences of Apostasia wallichii and Neuwiedia singapureana were sequenced and compared with 43 previously published photosynthetic orchid plastomes to characterize the plastome structure and evolution in the orchids...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29046294/local-ca-2-coupling-between-mitochondria-and-sarcoplasmic-reticulum-following-depolarization-in-guinea-pig-urinary-bladder-smooth-muscle-cells
#6
Hisao Yamamura, Keisuke Kawasaki, Sou Inagaki, Yoshiaki Suzuki, Yuji Imaizumi
Spatiotemporal changes in cytosolic Ca(2+) concentration ([Ca(2+)]c) trigger a number of physiological functions in smooth muscle cells (SMCs). We previously imaged Ca(2+)-induced Ca(2+) release following membrane depolarization as local Ca(2+) transients, Ca(2+) hotspots, in subplasmalemmal regions. In this study, the physiological significance of mitochondria on local Ca(2+) signaling was examined. Cytosolic and mitochondrial Ca(2+) images following depolarization or action potentials were recorded in single SMCs from the guinea pig urinary bladder using a fast-scanning confocal fluorescent microscope...
October 18, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29045724/effects-of-demographic-history-on-the-detection-of-recombination-hotspots-from-linkage-disequilibrium
#7
Amy L Dapper, Bret A Payseur
In some species, meiotic recombination is concentrated in small genomic regions. These "recombination hotspots" leave signatures in fine-scale patterns of linkage disequilibrium, raising the prospect that the genomic landscape of hotspots can be characterized from sequence variation. This approach has led to the inference that hotspots evolve rapidly in some species, but are conserved in others. Historic demographic events, such as population bottlenecks, are known to affect patterns of linkage disequilibrium across the genome, violating population genetic assumptions of this approach...
October 17, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29045126/characterization-of-three-druggable-hotspots-in-the-aurora-a-tpx2-interaction-using-biochemical-biophysical-and-fragment-based-approaches
#8
Patrick J McIntyre, Patrick M Collins, Lukáš Vrzal, Kristian Birchall, Laurence H Arnold, Chido Mpamhanga, Peter J Coombs, Selena G Burgess, Mark W Richards, Anja Winter, Václav Veverka, Frank von Delft, Andy Merritt, Richard Bayliss
The mitotic kinase Aurora-A and its partner protein TPX2 (Targeting Protein for Xenopus kinesin-like protein 2), are overexpressed in cancers and it has been proposed that they work together as an oncogenic holoenzyme. TPX2 is responsible for activating Aurora-A during mitosis, ensuring proper cell division. Disruption of the interface with TPX2 is therefore a potential target for novel anti-cancer drugs that exploit the increased sensitivity of cancer cells to mitotic stress. Here, we investigate the interface using co-precipitation assays and isothermal titration calorimetry to quantify the energetic contribution of individual residues of TPX2...
October 18, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/29044850/mesenchymal-stem-cells-therapy-a-promising-cell-based-therapy-for-treatment-of-myocardial-infraction
#9
REVIEW
Ayman El Sayed Shafei, Mahmoud Ahmed Ali, Hazem G Ghanem, Ahmed I Shehata, Ahmed A Abdelgawad, Hossam R Handal, Kareem A Talaat, Ahmed E Ashaal, Amal S El-Shal
AIM: For decades, mesenchymal stem (MSCs) cells had been used for cardiovascular diseases as regenerative therapy. This review is an attempt to summarize the types of MSCs involved in MI therapy, its possible mechanisms effects especially paracrine one in MI focusing on the studies (human and animal) conducted in the last 10 years. DISCUSSION: Recently, reports showed that MSC therapy could have infarct-limiting effects after myocardial infarction (MI) in both experimental and clinical trials...
October 16, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29043639/methods-to-study-repeat-fragility-and-instability-in-saccharomyces-cerevisiae
#10
Erica J Polleys, Catherine H Freudenreich
Trinucleotide repeats are common in the human genome and can undergo changes in repeat length. Expanded CAG repeats have been linked to over 14 human diseases and are considered hotspots for breakage and genomic rearrangement. Here, we describe two Saccharomyces cerevisiae based assays that evaluate the rate of chromosome breakage that occurs within a repeat tract (fragility), and a PCR-based assay to evaluate tract length changes (instability). The first fragility assay utilizes end-loss and subsequent telomere addition as the main mode of repair of a yeast artificial chromosome (YAC)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29043494/influence-of-proline-substitution-on-the-bioactivity-of-mammalian-derived-antimicrobial-peptide-nk-2
#11
Jiexi Yan, Xiaolei Liang, Chang Liu, Yuemei Cheng, Lanxia Zhou, Kairong Wang, Li Zhao
Multidrug-resistant bacteria are emerging as a global threat, making the search for alternative compounds urgent. Antimicrobial peptides (AMPs) became a promising hotspot due to their distinct action mechanism and possibility to be used as an alternative or complement to traditional antibiotics. However, gaining a better understanding about the relationship between antimicrobial peptides structure and its bioactivity is crucial for the development of next generation of antimicrobial agents. NK-2, derived from mammalian protein NK-lysin, has potent antitumor and bactericidal abilities...
October 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29043292/phyllodes-tumors-with-and-without-fibroadenoma-like-areas-display-distinct-genomic-features-and-may-evolve-through-distinct-pathways
#12
Fresia Pareja, Felipe C Geyer, Rahul Kumar, Pier Selenica, Salvatore Piscuoglio, Charlotte K Y Ng, Kathleen A Burke, Marcia Edelweiss, Melissa P Murray, Edi Brogi, Britta Weigelt, Jorge S Reis-Filho
Breast fibroepithelial lesions (fibroadenomas and phyllodes tumors) are underpinned by recurrent MED12 exon 2 mutations, which are more common in fibroadenomas and benign phyllodes tumors. TERT promoter hotspot mutations have been documented in phyllodes tumors, and found to be more frequent in borderline and malignant lesions. Several lines of evidence suggest that a subset of phyllodes tumors might arise from fibroadenomas. Here we sought to investigate the genetic differences between phyllodes tumors with fibroadenoma-like areas vs...
2017: NPJ Breast Cancer
https://www.readbyqxmd.com/read/29043050/prediction-of-biodiversity-hotspots-in-the-anthropocene-the-case-of-veteran-oaks
#13
Olav Skarpaas, Stefan Blumentrath, Marianne Evju, Anne Sverdrup-Thygeson
Over the past centuries, humans have transformed large parts of the biosphere, and there is a growing need to understand and predict the distribution of biodiversity hotspots influenced by the presence of humans. Our basic hypothesis is that human influence in the Anthropocene is ubiquitous, and we predict that biodiversity hot spot modeling can be improved by addressing three challenges raised by the increasing ecological influence of humans: (i) anthropogenically modified responses to individual ecological factors, (ii) fundamentally different processes and predictors in landscape types shaped by different land use histories and (iii) a multitude and complexity of natural and anthropogenic processes that may require many predictors and even multiple models in different landscape types...
October 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29042088/distribution-of-glyphosate-and-aminomethylphosphonic-acid-ampa-in-agricultural-topsoils-of-the-european-union
#14
Vera Silva, Luca Montanarella, Arwyn Jones, Oihane Fernández-Ugalde, Hans G J Mol, Coen J Ritsema, Violette Geissen
Approval for glyphosate-based herbicides in the European Union (EU) is under intense debate due to concern about their effects on the environment and human health. The occurrence of glyphosate residues in European water bodies is rather well documented whereas only few, fragmented and outdated information is available for European soils. We provide the first large-scale assessment of distribution (occurrence and concentrations) of glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) in EU agricultural topsoils, and estimate their potential spreading by wind and water erosion...
October 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29041452/in-situ-high-resolution-thermal-microscopy-on-integrated-circuits
#15
Guan-Yu Zhuo, Hai-Ching Su, Hsien-Yi Wang, Ming-Che Chan
The miniaturization of metal tracks in integrated circuits (ICs) can cause abnormal heat dissipation, resulting in electrostatic discharge, overvoltage breakdown, and other unwanted issues. Unfortunately, locating areas of abnormal heat dissipation is limited either by the spatial resolution or imaging acquisition speed of current thermal analytical techniques. A rapid, non-contact approach to the thermal imaging of ICs with sub-μm resolution could help to alleviate this issue. In this work, based on the intensity of the temperature-dependent two-photon fluorescence (TPF) of Rhodamine 6G (R6G) material, we developed a novel fast and non-invasive thermal microscopy with a sub-μm resolution...
September 4, 2017: Optics Express
https://www.readbyqxmd.com/read/29039242/computational-elucidation-mutational-and-hot-spot-based-designing-of-potential-inhibitors-against-human-acid-sensing-ion-channels-hasic-1a-to-treatment-of-various-physiological-conditions
#16
Anurag Singh Chauhan, Md Yousuf Ansari, Rani Mansuri, Manas Ranjan Dikhit, Vahab Ali, Ganesh Chandra Sahoo, Pradeep Das
Acid-sensing ion channels (ASICs) are ligand/proton-gated ion channels belonging to the family of the degenerin/epithelial Na(+) channel (DEG/ENaC). They function as a sodium-selective pore for Ca(2+) entry into neuronal cells during pathological conditions. The blocking of this channel has therapeutic importance, because at basal physiological pH (7.2), it is in a closed state and under a more acidic condition, and the ASIC1a ion channel is activated. To investigate the different states of the hASIC1a channel based on mutational analysis, structure based virtual screening and molecular dynamics simulation studies...
October 17, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29038591/genomics-alterations-of-metastatic-and-primary-tissues-across-15-cancer-types
#17
Gang Liu, Xiaohui Zhan, Chuanpeng Dong, Lei Liu
Metastasis is an important event for cancer evolution and prognosis. In this article, we analyzed the differences in genomic alterations between primary and metastatic tissues at hotspot regions in 15 cancer types and 10,456 samples. Differential somatic mutations at the amino acid, protein domain and gene levels, mutational exclusiveness, and copy number variations were identified in these cancers, while no significant nucleotide and gene fusion differences were detected. The homogeneity and heterogeneity of these differences in cancers were also detected...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038563/use-of-pulmonary-ct-angiography-with-low-tube-voltage-and-low-iodine-concentration-contrast-agent-to-diagnose-pulmonary-embolism
#18
Xuemei Hu, Liya Ma, Jinhua Zhang, Zhen Li, Yaqi Shen, Daoyu Hu
Pulmonary CT angiography (CTPA) is regarded as the preferred imaging method in diagnosing pulmonary embolism (PE). Considering the harm of radiation exposure and the side effect of iodinated contrast agent, CTPA protocol with low tube voltage and low dose of contrast agent became research hotspot in last decade. The present study evaluates the image quality, radiation dose, positive rate of PE and the location of PE with a CTPA protocol using low tube voltage (80 kVp) and low-iodine-concentration contrast agent (270 mg I/ml) in patients suspected of PE compared to a conventional CTPA protocol (120 kVp, 350 mg I/ml)...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038438/haplotype-analysis-of-xrcc2-gene-polymorphisms-and-association-with-increased-risk-of-head-and-neck-cancer
#19
Soma Saeed, Ishrat Mahjabeen, Romana Sarwar, Kashif Bashir, Mahmood Akhtar Kayani
We aimed to investigate the effect of hotspot variations of XRCC2 gene on the risk of head and neck cancer (HNC) in 400 patients and 400 controls. Five polymorphisms of XRCC2 gene G4234C (rs3218384), G4088T (rs3218373), G3063A (rs2040639), R188H (rs3218536) and rs7802034 were analyzed using Allele- specific polymerase chain reaction (ARMS-PCR) followed by sequence analysis. For rs3218373, the GG genotype indicated a statistically significant 3-fold increased risk of HNC (P < 0.001) after multivariate adjustment...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29037807/dicer1-related-sertoli-leydig-cell-tumor-and-gynandroblastoma-clinical-and-genetic-findings-from-the-international-ovarian-and-testicular-stromal-tumor-registry
#20
Kris Ann P Schultz, Anne K Harris, Michael Finch, Louis P Dehner, Jubilee B Brown, David M Gershenson, Robert H Young, Amanda Field, Weiying Yu, Joyce Turner, Nicholas G Cost, Dominik T Schneider, Douglas R Stewart, A Lindsay Frazier, Yoav Messinger, D Ashley Hill
BACKGROUND: Ovarian sex cord-stromal tumors (OSCST) include juvenile granulosa cell tumors (JGCT), Sertoli-Leydig cell tumor (SLCT) and gynandroblastoma (GAB) among others. These ovarian sex cord-stromal tumors as well as other tumors including pleuropulmonary blastoma (PPB) may be associated with DICER1 mutations. We sought to describe the clinical and genetic findings from the first 107 individuals enrolled in the International Ovarian and Testicular Stromal Tumor Registry. METHODS: Medical and family history were obtained for individuals consecutively enrolled in the International Ovarian and Testicular Stromal Tumor Registry...
October 13, 2017: Gynecologic Oncology
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