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https://www.readbyqxmd.com/read/28422083/nasopharyngeal-carriage-and-antimicrobial-susceptibility-patterns-of-streptococcus-pneumoniae-among-children-under-five-in-southwest-ethiopia
#1
Tiglu Gebre, Mulualem Tadesse, Dossegnaw Aragaw, Dagne Feye, Habtamu Bedimo Beyene, Dinberu Seyoum, Mekidim Mekonnen
Nasopharyngeal carriage of Streptococcus pneumoniae is found to play an important role in the development and transmission of pneumococcal diseases. In this study, we assessed the nasopharyngeal carriage, antimicrobial susceptibility patterns and associated risk factors of S. pneumoniae among children under five. A total of 361 children under five attending the outpatient department of Shanan Gibe Hospital in Jimma, Southwest Ethiopia were enrolled from June to September 2014. Nasopharyngeal specimens were collected using sterile plastic applicator rayon tipped swab and inoculated on tryptone soy agar supplemented with 5% sheep blood and 5 µg/mL gentamycin...
April 19, 2017: Children
https://www.readbyqxmd.com/read/28413862/a-simple-strategy-for-culturing-morphologically-conserved-rat-hypothalamic-tanycytes
#2
Pablo Nicolás De Francesco, Daniel Castrogiovanni, Maia Uriarte, Victoria Frassa, Francina Agosti, Jesica Raingo, Mario Perello
Hypothalamic tanycytes are specialized bipolar ependymal cells that line the floor of the third ventricle. Given their strategic location, tanycytes are believed to play several key functions including being a selective barrier and controlling the amount of hypothalamic-derived factors reaching the anterior pituitary. The in vitro culture of these cells has proved to be difficult. Here, we report an improved method for the generation of primary cultures of rat hypothalamic tanycytes. Ependymal cultures were derived from tissue dissected out of the median eminence region of 10-day-old rats and cultured in a chemically defined medium containing DMEM:F12, serum albumin, insulin, transferrin and the antibiotic gentamycin...
April 17, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28400006/microbiological-quality-of-selected-ready-to-eat-leaf-vegetables-sprouts-and-non-pasteurized-fresh-fruit-vegetable-juices-including-the-presence-of-cronobacter-spp
#3
Anna Berthold-Pluta, Monika Garbowska, Ilona Stefańska, Antoni Pluta
Bacteria of the genus Cronobacter are emerging food-borne pathogens. Foods contaminated with Cronobacter spp. may pose a risk to infants or adults with suppressed immunity. This study was aimed at determining the microbiological quality of ready-to-eat (RTE) plant-origin food products available on the Polish market with special emphasis on the prevalence of Cronobacter genus bacteria. Analyses were carried out on 60 samples of commercial RTE type plant-origin food products, including: leaf vegetables (20 samples), sprouts (20 samples) and non-pasteurized vegetable, fruit and fruit-vegetable juices (20 samples)...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399155/frequent-isolation-of-methicillin-resistant-staphylococcus-aureus-mrsa-st398-among-healthy-pigs-in-portugal
#4
Teresa Conceição, Hermínia de Lencastre, Marta Aires-de-Sousa
BACKGROUND: Although livestock-associated ST398 methicillin-resistant Staphylococcus aureus (MRSA) has been widely reported in different geographic regions, MRSA carriage studies among healthy pigs in Portugal are very limited. METHODS AND FINDINGS: In total, 101 swine nasal samples from two Portuguese farms were screened for MRSA. In addition five swine workers (including one veterinary and one engineer) and four household members were nasally screened. The isolates were characterized by spa typing, SCCmec typing and MLST...
2017: PloS One
https://www.readbyqxmd.com/read/28392528/colorimetric-microtiter-plate-assay-of-polycationic-aminoglycoside-antibiotics-in-culture-broth-using-amaranth
#5
Hajime Katano, Yasuhiro Kuroda, Shu Taira, Chitose Maruyama, Yoshimitsu Hamano
We present a colorimetric method for the detection of aminoglycoside antibiotics such as neomycin (NEO) using a reddish anionic dye, amaranth (AR(3-)). Under acidic conditions, at which NEO exists in fully protonated form (NEOH(6+)), the AR(3-) anion associates with the NEOH(6+) cation to form a precipitate, NEOH(AR)2. The precipitate was soluble in a buffer solution of pH 8.5, yielding a reddish solution with an absorption maximum at around 520 nm. Tobramycin and gentamycin, which exist as pentavalent cations under acidic conditions, gave almost the same results...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28391954/antimicrobial-susceptibility-of-pathogens-isolated-from-surgical-site-infections-in-japan-comparison-of-data-from-nationwide-surveillance-studies-conducted-in-2010-and-2014-2015
#6
Yoshio Takesue, Shinya Kusachi, Hiroshige Mikamo, Junko Sato, Akira Watanabe, Hiroshi Kiyota, Satoshi Iwata, Mitsuo Kaku, Hideaki Hanaki, Yoshinobu Sumiyama, Yuko Kitagawa, Toru Mizuguchi, Yoshiyasu Ambo, Masafumi Konosu, Keiichiro Ishibashi, Akihisa Matsuda, Kazuo Hase, Yasushi Harihara, Koji Okabayashi, Shiko Seki, Takuo Hara, Koshi Matsui, Yoichi Matsuo, Minako Kobayashi, Shoji Kubo, Kazuhisa Uchiyama, Junzo Shimizu, Ryohei Kawabata, Hiroki Ohge, Shinji Akagi, Masaaki Oka, Toshiro Wakatsuki, Katsunori Suzuki, Kohji Okamoto, Katsunori Yanagihara
A nationwide survey was conducted in Japan from 2014 to 2015 to investigate the antimicrobial susceptibility of pathogens isolated from surgical site infections (SSI). The resulting data were compared with that obtained in an earlier survey, conducted in 2010. Seven main organisms were collected, and 883 isolates were studied. A significant reduction in methicillin resistance was observed among Staphylococcus aureus isolates, dropping from 72.5% in 2010 to 53.8% in 2014-2015 (p < 0.001). MRSA isolates with a vancomycin minimum inhibitory concentration (MIC) of 2 μg/mL accounted for 1...
April 5, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28384865/lactobacillus-coryniformis-causing-pulmonary-infection-in-a-patient-with-metastatic-small-cell-carcinoma-case-report-and-review-of-literature-on-lactobacillus-pleuro-pulmonary-infections
#7
REVIEW
Priya Datta, Varsha Gupta, Gursimran Kaur Mohi, Jagdish Chander, Ashok Kumar Janmeja
Lactobacilli are normal commensals of the gastrointestinal and female genital tract. Due to its low virulence these bacteria are known to cause opportunistic infections. They cause mostly bacteraemia with or without endocarditis and rarely cause pleuro-pulmonary infection. We report a case of Lactobacillus coryniformis pleuro-pulmonary infection and review 14 previously reported cases of lactobacilli causing pleuro-pulmonary infections. Our patient had small cell carcinoma with metastasis. All the 14 cases had pre-existing risk factors like immunosuppresion, cancer or chronic disease...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28348776/first-case-report-of-pulmonary-nocardiosis-caused-by-nocardia-mexicana
#8
Tomokazu Kuchibiro, Takeshi Ikeda, Hirotaka Nakanishi, Yukiko Morishita, Katsuyuki Houdai, Junko Ito, Tohru Gonoi
INTRODUCTION: Nocardia species usually cause opportunistic infections, and the frequency of these infections is increasing owing to the growing population of immunocompromised hosts. However, Nocardia species may sometimes cause an infection disease in immunocompetent hosts. Nocardia mexicana infections are the least common and are very rare. CASE PRESENTATION: Herein, we report the first case of a pulmonary infection with N. mexicana in a 61-year-old Japanese woman with a history of hyperlipidaemia and bronchiectasis and a 6-month history of non-productive hacking cough...
August 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28343745/feasibility-of-sulfate-calcined-eggshells-for-removing-pathogenic-bacteria-and-antibiotic-resistance-genes-from-landfill-leachates
#9
Mao Ye, Mingming Sun, Xu Chen, Yanfang Feng, Jinzhong Wan, Kuan Liu, Da Tian, Manqiang Liu, Jun Wu, Arthur P Schwab, Xin Jiang
High abundance of human pathogen and antibiotic resistance genes (ARGs) in landfill leachate has become an emerging threat against human health. Therefore, sulfate- and calcination-modified eggshells as green agricultural bioresource were applied to test the feasibility of removing pathogenic bacteria and ARGs from leachate. The highest removal of Escherichia coli (E. coil) and gentamycin resistant gene (gmrA) from artificial contaminated landfill leachate was achieved by the application of eggshell with combined treatment of sulfate and calcination...
March 23, 2017: Waste Management
https://www.readbyqxmd.com/read/28342937/molecular-epidemiology-of-staphylococcus-aureus-nasal-colonization-among-patients-and-their-parents-guardian-in-an-iranian-referral-hospital
#10
Babak Pourakbari, Mahmoud Khodabandeh, Shima Mahmoudi, Farah Sabouni, Alireza Aziz-Ahari, Abbas Bahador, Sepideh Keshavarz Valian, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi
INTRODUCTION: Carriage of Staphylococcus aureus in the nose appears to play a key role in the epidemiology and pathogenesis of infection. It is important to investigate the genetic relatedness of S. aureus and MRSA clones in different geographic regions. The aim of this study was to assess the nasal carriage rate of S. aureus, including MRSA strains in both hospitalized children and general adult population (parents/guardian). In addition, antibiotic susceptibility pattern and molecular diversity of S...
March 22, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28337489/antibiotic-resistance-profiling-and-microbiota-of-the-upper-respiratory-tract-of-apparently-healthy-dogs-in-ibadan-south-west-nigeria
#11
Oluwafemi Babatunde Daodu, Elizabeth Adesola Amosun, Daniel Oladimeji Oluwayelu
BACKGROUND: Rearing of dogs and other pets has become increasingly popular in modern society. Bacterial flora resides within the nasal and oral cavities of dogs and when chanced, can be pathogenic. Certain similarities between humans and dogs portends dangerous behavioral habits that could lead to zoonotic disease transmission. This study was aimed at isolation, identification and antibiotic profiling of bacteria from nasal swabs of apparently healthy dogs. The zoonotic risk was also considered...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28323886/contribution-of-urinary-tract-infection-to-the-burden-of-febrile-illnesses-in-young-children-in-rural-kenya
#12
Wechuli Geoffrey Masika, Wendy Prudhomme O'Meara, Thomas L Holland, Janice Armstrong
INTRODUCTION: The clinical features of UTI in young children may not localize to the urinary tract and closely resemble other febrile illnesses. In malaria endemic areas, a child presenting with fever is often treated presumptively for malaria without investigation for UTI. Delayed or inadequate treatment of UTI increases the risk of bacteremia and renal scarring in young children and subsequently complications as hypertension and end stage renal disease in adulthood. METHODS: A cross-sectional study was carried out in a hospital in western Kenya...
2017: PloS One
https://www.readbyqxmd.com/read/28295617/carbapenemases-producing-klebsiella-pneumoniae-in-hospitals-of-two-regions-of-southern-italy
#13
Carla Calia, Carlo Pazzani, Marta Oliva, Maria Scrascia, Piero Lovreglio, Carmen Capolongo, Anna Maria Dionisi, Adriana Chiarelli, Rosa Monno
Carbapenem-resistant Klebsiella pneumoniae infections are reported with increasing frequency elsewhere in the world, representing a worrying phenomenon for global health. In Italy, there are hotspot data on the diffusion and type of carbapenemase-producing Enterobacteriaceae and K. pneumoniae in particular, with very few data coming from Apulia and Basilicata, two regions of Southern Italy. This study was aimed at characterizing by phenotypic and genotypic methods carbapenem-resistant K. pneumoniae isolated from several Hospitals of Apulia and Basilicata, Southern Italy...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28273964/biofilm-formation-by-drug-resistant-enterococci-isolates-obtained-from-chronic-periodontitis-patients
#14
Sonia Bhonchal Bhardwaj, Manjula Mehta, Shaveta Sood, Jyoti Sharma
INTRODUCTION: Enterococci are an important cause of opportunistic nosocomial infections and several multidrug resistant strains have emerged. The severity of periodontal diseases is managed by reduction in the pathogenic bacteria. There is a need to assess the prevalence and antibiotic susceptibility of enterococci colonizing the periodontal pocket and correlate its biofilm formation ability because oral biofilms provide a protective environment and are a reservoir of bacterial colonization of the gingival crevice...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28260752/antibiotic-resistance-new-challenge-in-the-management-of-bacterial-eye-infections
#15
A K Talukder, Z Sultana, I Jahan, M Khanam, M Rahman, M F Rahman, M B Rahman
Ophthalmologists are still facing difficulties in managing bacterial eye infections. The study was designed for the isolation and identification of bacteria from infected eyes and observation of the sensitivity and resistant pattern. This cross sectional study was performed among 160 patients of suspected bacterial eye infection at Dr. K. Zaman BNSB Eye Hospital, Mymensingh and Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh from March, 2010 to June, 2014. After collection of the samples from suspected infected eyes, it was nourished into nutrient broth in shaking incubator for three hours and then cultured into nutrient agar media followed by Mannitol salt agar, MacConkey's agar and blood agar...
January 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28253941/arthrodesis-of-the-ankle
#16
H Zwipp
About 90 % of all cases of painful posttraumatic ankle arthritis can be very successfully treated with a minor invasive ankle arthrodesis technique by using a small anterior approach and a fixation with four 6. 5 mm screws of which the posteromedial and transfibular one are inserted percutaneously. The results with this standardized procedure have been reported previously as excellent and good in a mid-term run of 6 years (34). This technique leads to a high union rate of 99% (92 of 93) with rapid bone healing within 8 ± 2 weeks, it causes a low minor complication rate of 8 % and enables a significant increase of the AOFAS ankle/hindfoot score (17) from 36 preoperatively to 85 postoperatively as well as a midtarsal movement of 24° ± 16°...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/28248681/risk-factors-for-infection-development-after-transrectal-prostate-biopsy-and-the-role-of-resistant-bacteria-in-colonic-flora
#17
Emine Dilek Eruz, Aysun Yalci, Eriz Ozden, Halide Aslaner, Suna Ogucu-Durgun, Deniz Derya Koseoglu-Taymur, Kemal Osman Memikoglu, Hakan Erdem, Halil Kurt
INTRODUCTION: In this study, we aimed to identify risk factors for the development of infectious complications after prostate biopsy and to investigate the role of intestinal colonization of bacteria that are resistant to prophylactic antibiotics. METHODOLOGY: A total of 168 patients who had undergone transrectal prostate biopsy (TRPB) under ciprofloxacin and gentamycin prophylaxis were included in the study. Stool cultures and subsequent antibiotic susceptibility testing were performed in all patients before the start of antibiotic prophylaxis...
February 28, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28248673/evaluation-of-methicillin-resistant-staphylococcus-aureus-nasal-carriage-in-malagasy-pig-and-poultry-non-industrial-farmers
#18
Tsiry Rasamiravaka, Tojo Tiana Andriatsitohanana, Andry Rasamindrakotroka
INTRODUCTION: The laboratory of Training and Research in Medical Biology of Madagascar conducted a cross-sectional study to estimate the rate of S. aureus nasal carriage of pig and poultry Malagasy farmers. METHODOLOGY: Pig and poultry farmers from capital town of Madagascar were selected for nasal swabs collection with information on potential risk factors for S. aureus colonization, including animal exposure. RESULTS: Nasal swabs from 180 farmers (M/F sex ratio: 0...
February 28, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28246454/molecular-identification-and-histopathological-study-of-natural-streptococcus-agalactiae-infection-in-hybrid-tilapia-oreochromis-niloticus
#19
A A Laith, Mohd Azmi Ambak, Marina Hassan, Shahreza Md Sheriff, Musa Nadirah, Ahmad Shuhaimi Draman, Wahidah Wahab, Wan Nurhafizah Wan Ibrahim, Alia Syafiqah Aznan, Amina Jabar, Musa Najiah
AIM: The main objective of this study was to emphasize on histopathological examinations and molecular identification of Streptococcus agalactiae isolated from natural infections in hybrid tilapia (Oreochromis niloticus) in Temerloh Pahang, Malaysia, as well as to determine the susceptibility of the pathogen strains to various currently available antimicrobial agents. MATERIALS AND METHODS: The diseased fishes were observed for variable clinical signs including fin hemorrhages, alterations in behavior associated with erratic swimming, exophthalmia, and mortality...
January 2017: Veterinary World
https://www.readbyqxmd.com/read/28218718/an-efficient-synthesis-of-novel-bioactive-thiazolyl-phthalazinediones-under-ultrasound-irradiation
#20
Fatma S Elsharabasy, Sobhi M Gomha, Thoraya A Farghaly, Heba S A Elzahabi
Novel 2-thiazolylphthalazine derivatives were efficiently synthesized under ultrasound irradiation, resulting in high yields and short reaction times after optimization of the reaction conditions. All prepared compounds were fully characterized using spectroscopic methods. They were screened for their antimicrobial activity against Gram-positive and Gram-negative bacteria as well as for antifungal activity. The antimicrobial activity profile of the tested compounds showed some promising results. The potent activity of compounds 4d, 7b (117% zone inhibition) and 7c (105% zone inhibition) against Salmonella sp...
February 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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