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Gentamycin

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https://www.readbyqxmd.com/read/27902273/racemicystis-persica-sp-nov-a-novel-myxobacterium-from-iranian-soil
#1
Joachim Wink, Azam Moradi, Gholam Hosein Ebrahimipour, Kathrin Mohr, Peter Kämpfer, Stefanie Glaeser, Fabienne Hennessen, Katja Gemperlein, Awal Prasad Ram, Corinna Wolf, Rolf Müller
A novel myxobacterium, strain MSr11462T, was isolated in 2015 from a soil sample collected from Kish Island beach, Persian Gulf, Iran. It displayed general myxobacterial features like Gram negative staining, rod shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting body like aggregates and myxospore like structures. The strain was mesophilic, aerobic and showed chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like gentamycin, polymyxin, Fusidic acid and trimethopril and the key fatty acids of whole cell hydrolysates were iso-C15:0, C16:0, iso-C17:0, C18:1 , iso-C17:1 2-OH, C18:1 2-OH, iso-C15:0 OAG (O-alkylglycerol), and C16:1 OAG...
November 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27889773/evaluation-of-kim-1-and-ngal-as-early-indicators-for-assessment-of-gentamycin-induced-nephrotoxicity-in-vivo-and-in-vitro
#2
Qi-Hui Luo, Meng-Lu Chen, Zheng-Li Chen, Chao Huang, An-Chun Cheng, Jing Fang, Li Tang, Yi Geng
BACKGROUND/AIMS: The aminolycoside Gentamicin is a widely used antibiotic, applied in equine medicine. Despite its clinical use, concerns remain regarding the potential toxic side-effects, such as nephrotoxicity. Early detection of renal damage is critical in preclinical drug development. This study was aimed to determine whether kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) may be early indicators in the assessment of Gentamycin-induced nephrotoxicity...
November 28, 2016: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/27886596/an-end-point-method-based-on-graphene-oxide-for-rnase-h-analysis-and-inhibitors-screening
#3
Chuan Zhao, Jialong Fan, Lan Peng, Lijian Zhao, Chunyi Tong, Wei Wang, Bin Liu
As a highly conserved damage repair protein, RNase H can hydrolysis DNA-RNA heteroduplex endonucleolytically and cleave RNA-DNA junctions as well. In this study, we have developed an accurate and sensitive RNase H assay based on fluorophore-labeled chimeric substrate hydrolysis and the differential affinity of graphene oxide on RNA strand with different length. This end-point measurement method can detect RNase H in a range of 0.01 to 1 units /mL with a detection limit of 5.0×10(-3) units/ mL under optimal conditions...
November 16, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27876960/characterization-and-control-of-symbiotic-bacillus-cereus-isolated-from-the-mid-gut-of-anopheles-subpictus-grassi
#4
Priyanka Mukhopadhyay, Soumendranath Chatterjee
An investigation was done to find out the role of gut bacterium on the larval development and survival of Anopheles subpictus, the vector responsible for the transmission of malaria. An. subpictus mosquitoes breed exclusively in stagnant water, including shrimp/fish ponds with high nutrient level. One bacterial strain (CX2) was isolated from the midgut of late third instar of mosquito larvae. The bacterial isolate was sensitive to recommended doses of tetracyclin (30 µg/disc), doxycycline (30 µg/disc), kanamycin (30 µg/disc), gentamycin (10 µg/disc), streptomycin (10 µg/disc), vancomycin (30 µg/disc), ofloxacin (5 µg/disc), levofloxacin (5 µg/disc), gatifloxacin (10 µg/disc), but resistant to ampicillin (10 µg/disc)...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27860210/limitation-of-the-antibiotic-eluting-bone-graft-substitute-an-example-of-gentamycin-impregnated-calcium-sulfate
#5
Chang-Chin Wu, Yang-Kai Huang, Wei-Jen Chang, Yun-Ching Wu, Chen-Chie Wang, Kai-Chiang Yang
Patients with inadequate volume of alveolar processes or bone defects commonly require graft substitutes in oral, maxillofacial or orthopedic surgery. Ridge augmentation and reconstruction of facial bony defects with bone graft materials achieve better outcomes in functional and aesthetic rehabilitation. The injectable calcium sulfate filler is used widely in intra-operative applications. Calcium sulfate bone filler has been shown to upregulate bone formation-related mRNA genes in vitro and improve osseointegration in vivo...
November 18, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27833719/antibiotic-resistance-properties-of-pseudomonas-aeruginosa-isolated-from-cases-of-superficial-infections-at-the-emergency-unit
#6
Koorosh Ahmadi, Amir Masoud Hashemian, Seyyed Mohsen Pouryaghobi, Reza Akhavan, Sara Rozmina, Ehsan Bolvardi
BACKGROUND: Pseudomonas aeruginosa, a ubiquitous opportunistic pathogen, is one of the main causative agents of human superficial infections. Infections due to these bacteria are difficult to heal and cause serious economic issues. OBJECTIVES: The present study was carried out to investigate the antibiotic resistance pattern of P. aeruginosa isolated from cases of superficial infections referred to the emergency health care units of Iranian Hospitals. MATERIALS AND METHODS: Three hundred swab samples were collected from patients with superficial infections...
January 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27822623/-esc-and-aha-guidelines-2015-on-endocarditis-in-competition-or-synchrony
#7
Bernhard Maisch
In the 2015 guidelines of the European Society of Cardiology (ESC) and the American Heart Association (AHA) on infective endocarditis, the diagnostics are based on the modified Duke criteria. The diagnosis can be confirmed by a combination of micro-organisms demonstrated in culture or in situ, with the detection of valvular lesions or abscess formation by an imaging modality using echocardiography, positron emission tomography computed tomography (PET/CT), cardio-CT or nuclear medical methods. The management should be further improved by an interdisciplinary endocarditis team in a specifically designated reference center...
December 2016: Herz
https://www.readbyqxmd.com/read/27822247/pfge-genotyping-and-molecular-characterization-of-campylobacter-spp-isolated-from-chicken-meat
#8
B Bakhshi, M Kalantar, A Rastegar-Lari, F Fallah
A total of 70 samples were collected from chicken meat obtained from 10 markets in Tehran, Iran from which 39 Campylobacter coli were isolated. Among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (SXT) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). No resistance was to gentamycin was observed. None of the Campylobacter strains under study harbored integron, suggesting the involvement of other resistance mechanisms in emergence of multi drug resistance (MDR) phenotype among the isolates...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822242/an-outbreak-of-yolk-sac-infection-and-dead-in-shell-mortality-in-common-canary-serinus-canaria-caused-by-klebsiella-pneumoniae
#9
J Razmyar, A H Zamani
Yolk sac infection (YSI) and dead-in-shell mortality caused by Enterobacteriaceae in birds are not a rare phenomenon, however there are only a few reports indicating the association between these conditions and Klebsiella spp. among canary chicks (Serinus canaria). There have been reports of high mortality among 1-3 day old canary chicks in an indoor flock of canaries. In order to study the causative agent, yolk sac samples from dead-in-shell and day-old canary chicks were cultured. Klebsiella pneumonia was isolated and identified based on biochemical tests and using genus and species-specific multiplex PCR and later tested for their susceptibility to 13 antimicrobial agents...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27819649/the-effect-of-nystatin-solution-on-otoacoustic-emissions-in-rats
#10
Alperen Vural, Mehmet İlhan Şahin, Mesut Aydın, Ramazan Gündoğdu, Turan Arlı, Hacı Okuducu, Denizhan Dizdar, İsmail Külahlı
OBJECTIVE: In patients with a perforated tympanic membrane, topically administered medication reaches the middle ear and thus creates a risk of ototoxicity. The aim of the present study was to evaluate the possible ototoxic effect of the antifungal medication nystatin when administered to the rat middle ear cavity. MATERIALS AND METHODS: Three groups (negative control, positive control, and study groups), each containing eight rats, were formed. Before the drug administration, distortion product otoacoustic emissions were recorded in both ears of each rat...
November 7, 2016: Journal of International Advanced Otology
https://www.readbyqxmd.com/read/27803461/synthesis-and-antimicrobial-activity-of-nitrobenzyl-oxy-phenol-derivatives
#11
Malik Suliman Mohamed, Toshihide Maki, Mohammad Monir Shah, Yoshio Ichinose
Two hydroquinone derivatives were prepared and their antimicrobial activity evaluated. Their minimum inhibitory concentrations (MICs) were determined using a broth dilution method. Gentamycin and ciprofloxacin were used as reference antibiotics. The antimicrobial activity of 4-(benzyloxy)phenol (monobenzone) was also evaluated based on its structural similarity to the new compounds; activity was comparable to that of 3,5-dimethyl-4-((4-nitrobenzyl)oxy)phenol (4a). 2,3,5-Trimethyl-4-((4-nitrobenzyl)oxy)phenol (4b) exhibited the best antibacterial activity against both clinical isolates and type strain of Moraxella catarrhalis (M...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27766173/multidrug-resistance-of-acinetobacter-baumannii-in-ladoke-akintola-university-teaching-hospital-osogbo-nigeria
#12
G Odewale, O J Adefioye, J Ojo, F A Adewumi, O A Olowe
Acinetobacter baumannii is a ubiquitous pathogen that has emerged as a major cause of healthcare-associated infections at Ladoke Akintola University Teaching Hospital. Isolates were assayed according to standard protocol. The isolates were subjected to molecular techniques to detect blaOXA, blaTEM, blaCTX-M, and blaSHV genes in strains of the A. baumannii isolates. The prevalence of A. baumannii was 8.5% and was most prevalent among patients in the age group 51-60 (36%); the male patients (63.6%) were more infected than their female counterparts...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27731830/detection-of-antibiotic-residues-in-poultry-meat
#13
Abdul Sajid, Natasha Kashif, Nasira Kifayat, Shabeer Ahmad
The antibiotic residues in poultry meat can pose certain hazards to human health among them are sensitivity to antibiotics, allergic reactions, mutation in cells, imbalance of intestinal micro biota and bacterial resistance to antibiotics. The purpose of the present paper was to detect antibiotic residue in poultry meat. During the present study a total of 80 poultry kidney and liver samples were collected and tested for detection of different antibiotic residues at different pH levels Eschericha coli at pH 6, 7 and Staphyloccocus aureus at pH 8 & 9...
September 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27721784/corneal-ring-infiltrates-caused-by-serratia-marcescens-in-a-patient-with-human-immunodeficiency-virus
#14
Winai Chaidaroon, Sumet Supalaset
PURPOSE: To describe corneal ring infiltrates caused by Serratia marcescens in a patient with human immunodeficiency virus (HIV-1) who wore contact lenses. METHODS: A case study of a patient with keratitis due to an infection caused by S. marcescens and exhibiting corneal ring infiltrates was reviewed for history, clinical manifestation, microscopic study, and management. RESULTS: A 29-year-old man who had a history of contact lens wear and HIV-1 infection was admitted to hospital because of blurred vision, redness, and corneal infiltrates in the shape of a ring in the left eye...
May 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/27676251/super-anticoagulant-heparin-mimicking-hydrogel-thin-film-attached-substrate-surfaces-to-improve-hemocompatibility
#15
Min He, Xiaofei Cui, Huiyi Jiang, Xuelian Huang, Weifeng Zhao, Changsheng Zhao
In this study, heparin-mimicking hydrogel thin films are covalently attached onto poly(ether sulfone) membrane surfaces to improve anticoagulant property. The hydrogel films display honeycomb-like porous structure with well controlled thickness and show long-term stability. After immobilizing the hydrogel films, the membranes show excellent anticoagulant property confirmed by the activated partial thromboplastin time values exceeding 600 s. Meanwhile, the thrombin time values increase from 20 to 61 s as the sodium allysulfonate proportions increase from 0 to 80 mol%...
September 27, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27669285/prevalence-of-methicillin-resistant-staphylococcus-aureus-and-other-staphylococcus-species-in-raw-meat-samples-intended-for-human-consumption-in-benin-city-nigeria-implications-for-public-health
#16
Etinosa O Igbinosa, Abeni Beshiru, Lucy U Akporehe, Faith E Oviasogie, Owen O Igbinosa
The present study was designed to characterize methicillin-resistant staphylococci from raw meat. A total of 126 meat samples were obtained from open markets between February and April, 2015. Antimicrobial susceptibility testing was carried out using the disc diffusion method. Molecular profiling was conducted using 16S rRNA, mecA, nuc, and PVL gene signatures were detected by polymerase chain reaction assay. Fifty isolates of methicillin-resistant Staphylococcus spp. were detected in 26 (52%) pork, 14 (28%) beef and 10 (20%) chicken samples...
September 24, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27660816/isolation-identification-and-antibiotic-susceptibility-testing-of-salmonella-from-slaughtered-bovines-and-ovines-in-addis-ababa-abattoir-enterprise-ethiopia-a-cross-sectional-study
#17
Abe Kebede, Jelalu Kemal, Haile Alemayehu, Solomon Habte Mariam
Salmonellae are ubiquitous, found in animals, humans, and the environment, a condition which facilitates transmission and cross contamination. Salmonella enterica serotypes exert huge health and economic impacts due to their virulence or carriage of antibiotic resistance traits. To address this significant issues with regard to public health, availability of adequate information on the prevalence and antibiotic resistance patterns of Salmonella, and establishment of adequate measures to control contamination and infection are needed...
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/27659717/antibiotic-resistance-patterns-and-genetic-relatedness-of-enterococcus-faecalis-and-e-faecium-isolated-from-military-working-dogs-in-korea
#18
Kiman Bang, Jae-Uk An, Woohyun Kim, Hee-Jin Dong, Junhyung Kim, Seongbeom Cho
Enterococcus spp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. To investigate whether this is a potential risk in military working dogs (MWDs), we analyzed antibiotic resistance patterns and genetic relatedness of Enterococcus spp. Isolated from fecal samples of MWDs of four different age groups. The isolation rates of Enterococcus spp., and specifically of E...
September 1, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27650470/repeated-courses-of-intratympanic-dexamethasone-injection-are-effective-for-intractable-meniere-s-disease
#19
Yangming Leng, Bo Liu, Renhong Zhou, Jingjing Liu, Dongdong Liu, Su-Lin Zhang, Wei-Jia Kong
CONCLUSION: Intra-tympanic dexamethasone injection (ITD) could serve as a first-line intra-tympanic (IT) treatment for Meniere's disease (MD), regardless of hearing level. Even if the response to initial ITD course was unsatisfactory, a repeated course may be beneficial with some patients. OBJECTIVES: This study examined the effect of repeated courses of ITD administered on demand and investigated the possibility of ITD as an initial IT treatment for medically intractable MD patients...
September 21, 2016: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27631009/establishment-of-simple-and-routine-methods-in-early-diagnosis-of-gentamicin-induced-kidney-injury-based-on-a-rat-model
#20
Cuiyan Liu, Youxi Kang, Huiqin Zhang, Long Zhu, Hai Yu, Chunyang Han
The changes in biomarkers of gentamycin- (GM-) induced kidney injury have been studied by using simple and routine methods and also assessed the efficacy and utility of these routine biomarkers in early diagnosis. Eighty Sprague-Dawley (SD) rats were randomly divided into 4 groups: three experimental groups treated with different GM dosages (4, 20, and 100 mg·kg(-1)) and a control group. The experimental groups were given intramuscular GM injections once daily for 14 days, and the control group was given intramuscular sterile water...
2016: BioMed Research International
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