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https://www.readbyqxmd.com/read/28203289/epidemiological-molecular-characterization-and-antibiotic-resistance-of-salmonella-enterica-serovars-isolated-from-chicken-farms-in-egypt
#1
Hanem El-Sharkawy, Amin Tahoun, Abd El-Galiel A El-Gohary, Moshira El-Abasy, Fares El-Khayat, Trudi Gillespie, Yukio Kitade, Hafez M Hafez, Heinrich Neubauer, Hosny El-Adawy
BACKGROUND: Salmonella is one of major causes of foodborne outbreaks globally. This study was conducted to estimate the prevalence, typing and antibiotic susceptibilities of Salmonella enterica serovars isolated from 41 broiler chicken farms located in Kafr El-Sheikh Province in Northern Egypt during 2014-2015. The clinical signs and mortalities were observed. RESULTS: In total 615 clinical samples were collected from broiler flocks from different organs (liver, intestinal content and gall bladder)...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28192644/perioperative-antibiotics-for-prevention-of-acute-endophthalmitis-after-cataract-surgery
#2
REVIEW
Emily W Gower, Kristina Lindsley, Samantha E Tulenko, Afshan A Nanji, Ilya Leyngold, Peter J McDonnell
BACKGROUND: Endophthalmitis is a severe inflammation of the anterior or posterior (or both) chambers of the eye that may be sterile or associated with infection. It is a potentially vision-threatening complication of cataract surgery. Prophylactic measures for endophthalmitis are targeted against various sources of infection. OBJECTIVES: To evaluate the effects of perioperative antibiotic prophylaxis for endophthalmitis following cataract surgery compared with no prophylaxis or other form of prophylaxis...
February 13, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28187694/antibacterial-activity-of-aqueous-and-methanol-extracts-of-selected-species-used-in-livestock-health-management
#3
Clarice P Mudzengi, Amon Murwira, Musa Tivapasi, Chrispen Murungweni, Joan V Burumu, Tinyiko Halimani
CONTEXT: Salvadora persica L. (Salvadoraceae), Colophospermum mopane (J.Kirk ex Benth.) J. Léonard (Leguminosae) and Dichrostachys cinerea (L.) Wight & Arn. (Leguminosae) crude extracts are used by local farmers against many livestock infections with little or no side effects usually associated with synthetic antimicrobials. However, their efficacy has rarely been tested. OBJECTIVE: These plants were tested for potential antibacterial activity against clinical isolates of Staphylococcus aureus ATCC33862 and Escherichia coli ATCC25922...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28185962/design-and-evaluation-of-antibiotic-releasing-self-assembled-scaffolds-at-room-temperature-using-biodegradable-polymer-particles
#4
Prasad Admane, Jatin Gupta, Ancy Ij, Robin Kumar, Amulya K Panda
Biodegradable polymer based drug eluting implants offer many advantages such as predictable drug release kinetics, safety, and acceptable drug loading under ambient conditions. Herein, we describe fabrication and evaluation of antibiotic loaded scaffolds for localized delivery and tissue engineering applications. PDLLA particles entrapping gentamycin were formulated using solvent evaporation method and used for scaffold fabrication. Optimization of formulation parameters such as pH of the internal aqueous phase and combination of excipients like glycerol, polyvinyl alcohol (PVA) resulted in high entrapment efficiencies up to 96% of gentamicin in particles with drug load of 16-18μg/mg of polymer particles...
February 6, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28160117/characterization-of-salmonella-spp-from-wastewater-used-for-food-production-in-morogoro-tanzania
#5
Ofred J Mhongole, Robinson H Mdegela, Lughano J M Kusiluka, Anita Forslund, Anders Dalsgaard
Wastewater use for crop irrigation and aquaculture is commonly practiced by communities situated close to wastewater treatment ponds. The objective of this study was to characterize Salmonella spp. and their antimicrobial susceptibility patterns among isolates from wastewater and Tilapia fish. A total of 123 Salmonella spp. isolates were isolated from 52 water and 21 fish intestinal samples. Genotyping of Salmonella spp. isolates was done by Pulsed-field Gel Electrophoresis (PFGE). Antimicrobial susceptibility testing was done by the minimal inhibitory concentration (MIC) technique...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28150571/sanguibacter-gelidistatuariae-sp-nov-a-novel-psychrotolerant-anaerobe-from-an-ice-sculpture-in-antarctica-and-emendation-of-descriptions-for-the-family-sanguibacteraceae-the-genus-sanguibacter-and-species-s-antarcticus-s-inulinus-s-kedieii-s-marinus-s-soli
#6
Elena V Pikuta, Zhe Lyu, Melissa D Williams, Nisha B Patel, Yuchen Liu, Richard Brice Hoover, Hans-Jürgen Busse, Paul A Lawson, William B Whitman
A novel psychrotolerant bacterium strain ISLP-3T was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase positive. Growth occurred at 3-35 ºC with an optimum at 24 °C, 0-2 % w/v NaCl with an optimum at 0.3 % and pH 6.2 - 9.5 with an optimum 7.5. Strain ISLP-3T grew on several carbon sources, with the best growth on D-cellobiose...
February 1, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28148282/optimization-of-an-artificial-tick-feeding-assay-for-dermacentor-reticulatus
#7
Christoph Krull, Bettina Böhme, Peter-Henning Clausen, Ard M Nijhof
BACKGROUND: The development of standardized in vitro feeding methods for ixodid ticks has been hampered by their complex feeding behaviour and the long duration of their blood meal. In this study, we aimed to optimize several parameters for the in vitro feeding of adult Dermacentor reticulatus. METHODS: Ticks were fed on heparinized bovine blood collected at a slaughterhouse, using a modified silicone membrane feeding assay. Effects on tick feeding and fecundity of different blood meal treatments (freezing, irradiation, addition of antibiotics), ambient conditions (increased CO2 concentration) and phagostimulant use (addition of 2 g/l and 4 g/l glucose to the blood meal) were systematically evaluated...
February 2, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28130129/heparin-mimetic-polyurethane-hydrogels-with-anticoagulant-tunable-mechanical-property-and-controllable-drug-releasing-behavior
#8
Yuan Chen, Rui Wang, Yonghui Wang, Weifeng Zhao, Shudong Sun, Changsheng Zhao
In the present study, novel heparin-mimetic polyurethane hydrogels were prepared by introducing chemical crosslinked sulfated konjac glucomannan (SKGM). Scanning electron microscopy (SEM) results indicated that the introduction of SKGM and the increase of the molecular weight of diol segments could enlarge the pore sizes of the hydrogels. The swelling behavior corresponded with the SEM results, and the hydrogels could absorb more water after the modification. The modification also led to an improvement in the mechanical property...
January 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28123297/a-decomposable-silica-based-antibacterial-coating-for-percutaneous-titanium-implant
#9
Jia Wang, Guofeng Wu, Xiangwei Liu, Guanyang Sun, Dehua Li, Hongbo Wei
Although percutaneous titanium implants have become one of the best choices as retainers in the facial defects, peri-implantitis still occurs at a significant rate. This unwanted complication occurs due to adhesion of bacteria and subsequent biofilm formation. To solve this problem, we have developed a novel antibiotic nanodelivery system based on self-decomposable silica nanoparticles. In this study, silica-gentamycin (SG) nanoparticles were successfully fabricated using an innovative one-pot solution. The nanoparticles were incorporated within a gelatin matrix and cross-linked on microarc-oxidized titanium...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28122502/occurrence-of-escherichia-coli-o157-h7-in-cattle-feces-and-contamination-of-carcass-and-various-contact-surfaces-in-abattoir-and-butcher-shops-of-hawassa-ethiopia
#10
Biruhtesfa Atnafie, Degmawi Paulos, Mesele Abera, Genene Tefera, Dereje Hailu, Surafel Kasaye, Kebede Amenu
BACKGROUND: Despite of the sanitation measures in municipal abattoirs to reduce contamination, Escherichia coli continues to be a health hazard. The present study was conducted on 150 apparently healthy slaughtered cattle at municipal abattoir and in 50 different butcher shops in Hawassa town, Ethiopia. The objectives of the study were investigating the occurrence and antimicrobial resistance of E. coli O157:H7 isolated from fecal samples, carcasses swab, contacts surfaces (swabs of meat handlers hands, knife and clothes of meat transporters) as well as from butcher shops (meat samples, swabs from cutting board swab, butcher men hand and knife surface)...
January 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28109187/antimicrobial-and-antioxidant-activities-of-saccharomyces-cerevisiae-ifst062013-a-potential-probiotic
#11
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Probiotic yeast has become a field of interest to scientists in recent years. METHODS: Conventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties. RESULTS: This study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme...
January 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28088690/electrochemical-studies-of-dna-interaction-and-antimicrobial-activities-of-mn-ii-fe-iii-co-ii-and-ni-ii-schiff-base-tetraazamacrocyclic-complexes
#12
Anuj Kumar, Vinod Kumar Vashistha, Prashant Tevatia, Randhir Singh
Tetraazamacrocyclic complexes of Mn(II), Fe(III), Co(II) and Ni(II) have been synthesized by template method. These tetraazamacrocycles have been analyzed with various techniques like molar conductance, IR, UV-vis, mass spectral and cyclic voltammetric studies. On the basis of all these studies, octahedral geometry has been assigned to these tetraazamacrocyclic complexes. The DNA binding properties of these macrocyclic complexes have been investigated by electronic absorption spectra, fluorescence spectra, cyclic voltammetric and differential pulse voltammetric studies...
January 6, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28081583/-early-results-of-adjuvant-topical-treatment-of-recurrent-osteomyelitis-with-absorbable-antibiotic-carriers
#13
Y Gramlich, G Walter, J Gils, R Hoffmann
Background Treatment of musculoskeletal infections principally consists of radical surgical debridement and systemic administration of antibiotics. Additional local antibiotic therapy is not yet generally established, and lacks evidence-based proof of efficacy. Nonetheless, there are a variety of practical approaches, as most specialised departments are unwilling to forego this option. The established polymethylmetacrylate (PMMA) carrier system has a number of practical disadvantages. This has led to the increased use of absorbable carrier systems, and those based on calcium sulphate have given particularly encouraging results...
January 12, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/28074125/shiga-vero-toxin-producing-escherichia-coli-isolated-from-the-hospital-foods-virulence-factors-o-serogroups-and-antimicrobial-resistance-properties
#14
Reza Ranjbar, Mojtaba Masoudimanesh, Farhad Safarpoor Dehkordi, Nematollah Jonaidi-Jafari, Ebrahim Rahimi
BACKGROUND: According to the presence of the weak, diabetic and immunosuppressive patients in hospitals, hospital foods should have a high quality and safety. Cooking a lot of foods higher than daily requirement, storage of cooked foods in an inappropriate condition and presence of nurses and servants in distribution of food to patients are the main reasons caused contamination of hospital foods. Shiga toxigenic Escherichia coli is one of the common cause of food poisoning in hospitals...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28070047/-clinical-analysis-for-patients-with-continuous-ambulatory-peritoneal-dialysis-associated-peritonitis
#15
Jian Liu, Xun Huang, Yao Liu, Hui Xu, Rui'e Gong, Chunhui Li
To analyze the clinical characteristics of continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis in the tertiary hospitals and to discuss the preventive and therapeutic strategy.
 Methods: The clinical characteristics, pathogens, resistance and outcomes of 126 CAPD associated peritonitis in 104 patients from Jan, 2013 to June, 2016, were retrospectively analyzed.
 Results: Among the patients, the incidence rates of abdominal pain, fever, diarrhea and emesis were 104 (82.54%), 56 (44.44%), 49 (38...
December 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28053260/comparison-of-the-elution-properties-of-commercially-available-gentamicin-and-bone-cement-containing-vancomycin-with-home-made-preparations
#16
COMPARATIVE STUDY
N M Frew, T Cannon, T Nichol, T J Smith, I Stockley
AIMS: Vancomycin is commonly added to acrylic bone cement during revision arthroplasty surgery. Proprietary cement preparations containing vancomycin are available, but are significantly more expensive. We investigated whether the elution of antibiotic from 'home-made' cement containing vancomycin was comparable with more expensive commercially available vancomycin impregnated cement. MATERIALS AND METHODS: A total of 18 cement discs containing either proprietary CopalG+V; or 'home-made' CopalR+G with vancomycin added by hand, were made...
January 2017: Bone & Joint Journal
https://www.readbyqxmd.com/read/28033700/brucellosis-with-multi-organ-involvement-in-a-patient-with-history-of-composite-aortic-graft-and-hepatitis-b
#17
Seyed Ali Dehghan Manshadi, Omid Rezahosseini, Zahra Abdi Liaei
The brucellosis with multi-organ involvement in a patient with a history of the composite aortic graft (Bentall procedure) and Hepatitis B infection is rare. A 35-year-old man presented to us with fever and loss of consciousness. Four years ago, he was IDU and underwent cardiac surgery because of endocarditis. Recently lumbar spondylodiscitis was diagnosed. The Wright (1/320) and Coombs Wright tests (1/640) were positive. After CNS imaging, lumbar puncture was done. The CSF pleocytosis was lymphocyte dominant...
November 2016: Acta Medica Iranica
https://www.readbyqxmd.com/read/28030576/functional-and-structural-analysis-of-a-highly-expressed-yersinia-pestis-small-rna-following-infection-of-cultured-macrophages
#18
Nan Li, Scott P Hennelly, Chris J Stubben, Sofiya Micheva-Viteva, Bin Hu, Yulin Shou, Momchilo Vuyisich, Chang-Shung Tung, Patrick S Chain, Karissa Y Sanbonmatsu, Elizabeth Hong-Geller
Non-coding small RNAs (sRNAs) are found in practically all bacterial genomes and play important roles in regulating gene expression to impact bacterial metabolism, growth, and virulence. We performed transcriptomics analysis to identify sRNAs that are differentially expressed in Yersinia pestis that invaded the human macrophage cell line THP-1, compared to pathogens that remained extracellular in the presence of host. Using ultra high-throughput sequencing, we identified 37 novel and 143 previously known sRNAs in Y...
2016: PloS One
https://www.readbyqxmd.com/read/27993865/simultaneous-determination-of-five-specific-and-sensitive-nephrotoxicity-biomarkers-in-serum-and-urine-samples-of-four-drug-induced-kidney-injury-models
#19
Liqiang Gu, Huiyan Shi, Ruowen Zhang, Zhe Wei, Kai-Shun Bi, Xiao-Hui Chen
In clinical diagnosis, serum creatinine (CR) was commonly used as the routine markers for the evaluation of kidney injury. To investigate the specific and sensitive nephrotoxicity biomarkers in different drug-induced kidney injury (DKI) models, receiver operating characteristic (ROC) analysis was applied in this study. The quantification data were acquired by the LC-MS determination. Histopathology results showed that different kinds of kidney injuries were observed in different DKI models (gentamycin, cisplatin, methotrexate and amphotericin B models), indicating the injuries might be caused by different mechanisms...
January 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27959419/antimicrobial-resistance-development-following-surgical-site-infections
#20
Daniela Călina, Anca Oana Docea, Lucica Rosu, Ovidiu Zlatian, Alexandra Floriana Rosu, Florin Anghelina, Otilia Rogoveanu, Andreea Letiția Arsene, Alina Crenguța Nicolae, Cristina Manuela Drăgoi, John Tsiaoussis, Aristides M Tsatsakis, Demetrios A Spandidos, Nikolaos Drakoulis, Eliza Gofita
Surgical site infections (SSIs) determine an increase in hospitalization time and antibiotic therapy costs. The aim of this study was to identify the germs involved in SSIs in patients from the Clinical Emergency County Hospital of Craiova (SCJUC) and to assess their resistance to antimicrobials, with comparisons between surgical wards and the intensive care unit (ICU). The biological samples were subjected to classical bacteriological diagnostics. Antibiotic resistance was tested by disc diffusion. We used hierarchical clustering as a method to group the isolates based upon the antibiotic resistance profile...
February 2017: Molecular Medicine Reports
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