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Pengbo Liang, Thomas F Stratil, Claudia Popp, Macarena Marín, Jessica Folgmann, Kirankumar S Mysore, Jiangqi Wen, Thomas Ott
Plant cell infection is tightly controlled by cell surface receptor-like kinases (RLKs). Like other RLKs, the Medicago truncatula entry receptor LYK3 laterally segregates into membrane nanodomains in a stimulus-dependent manner. Although nanodomain localization arises as a generic feature of plant membrane proteins, the molecular mechanisms underlying such dynamic transitions and their functional relevance have remained poorly understood. Here we demonstrate that actin and the flotillin protein FLOT4 form the primary and indispensable core of a specific nanodomain...
April 30, 2018: Proceedings of the National Academy of Sciences of the United States of America
Dhileepkumar Jayaraman, Alicia L Richards, Michael S Westphall, Joshua J Coon, Jean-Michel Ané
Detecting the phosphorylation substrates of multiple kinases in a single experiment is a challenge, and new techniques are being developed to overcome this challenge. Here, we used a multiplexed assay for kinase specificity (MAKS) to identify the substrates directly and to map the phosphorylation site(s) of plant symbiotic receptor-like kinases. The symbiotic receptor-like kinases nodulation receptor-like kinase (NORK) and lysin motif domain-containing receptor-like kinase 3 (LYK3) are indispensable for the establishment of root nodule symbiosis...
June 2017: Plant Journal: for Cell and Molecular Biology
Kana Miyata, Masahiro Hayafune, Yoshihiro Kobae, Hanae Kaku, Yoko Nishizawa, Yoshiki Masuda, Naoto Shibuya, Tomomi Nakagawa
In legume-specific rhizobial symbiosis, host plants perceive rhizobial signal molecules, Nod factors, by a pair of LysM receptor-like kinases, NFR1/LYK3 and NFR5/NFP, and activate symbiotic responses through the downstream signaling components also required for arbuscular mycorrhizal (AM) symbiosis. Recently, the rice NFR1/LYK3 ortholog, OsCERK1, was shown to play crucial roles for AM symbiosis. On the other hand, the roles of the NFR5/NFP ortholog in rice have not been elucidated, while it has been shown that NFR5/NFP orthologs, Parasponia PaNFR5 and tomato SlRLK10, engage in AM symbiosis...
November 2016: Plant & Cell Physiology
Tatiana Vernié, Sylvie Camut, Céline Camps, Céline Rembliere, Fernanda de Carvalho-Niebel, Malick Mbengue, Ton Timmers, Virginie Gasciolli, Richard Thompson, Christine le Signor, Benoit Lefebvre, Julie Cullimore, Christine Hervé
PUB1, an E3 ubiquitin ligase, which interacts with and is phosphorylated by the LYK3 symbiotic receptor kinase, negatively regulates rhizobial infection and nodulation during the nitrogen-fixing root nodule symbiosis in Medicago truncatula In this study, we show that PUB1 also interacts with and is phosphorylated by DOES NOT MAKE INFECTIONS 2, the key symbiotic receptor kinase of the common symbiosis signaling pathway, required for both the rhizobial and the arbuscular mycorrhizal (AM) endosymbioses. We also show here that PUB1 expression is activated during successive stages of root colonization by Rhizophagus irregularis that is compatible with its interaction with DOES NOT MAKE INFECTIONS 2...
April 2016: Plant Physiology
Estíbaliz Larrainzar, Brendan K Riely, Sang Cheol Kim, Noelia Carrasquilla-Garcia, Hee-Ju Yu, Hyun-Ju Hwang, Mijin Oh, Goon Bo Kim, Anandkumar K Surendrarao, Deborah Chasman, Alireza F Siahpirani, Ramachandra V Penmetsa, Gang-Seob Lee, Namshin Kim, Sushmita Roy, Jeong-Hwan Mun, Douglas R Cook
The legume-rhizobium symbiosis is initiated through the activation of the Nodulation (Nod) factor-signaling cascade, leading to a rapid reprogramming of host cell developmental pathways. In this work, we combine transcriptome sequencing with molecular genetics and network analysis to quantify and categorize the transcriptional changes occurring in roots of Medicago truncatula from minutes to days after inoculation with Sinorhizobium medicae. To identify the nature of the inductive and regulatory cues, we employed mutants with absent or decreased Nod factor sensitivities (i...
September 2015: Plant Physiology
Xiaowei Zhang, Wentao Dong, Jongho Sun, Feng Feng, Yiwen Deng, Zuhua He, Giles E D Oldroyd, Ertao Wang
The establishment of symbiotic interactions between mycorrhizal fungi, rhizobial bacteria and their legume hosts involves a common symbiosis signalling pathway. This signalling pathway is activated by Nod factors produced by rhizobia and these are recognised by the Nod factor receptors NFR1/LYK3 and NFR5/NFP. Mycorrhizal fungi produce lipochitooligosaccharides (LCOs) similar to Nod factors, as well as short-chain chitin oligomers (CO4/5), implying commonalities in signalling during mycorrhizal and rhizobial associations...
January 2015: Plant Journal: for Cell and Molecular Biology
Sjef Moling, Anna Pietraszewska-Bogiel, Marten Postma, Elena Fedorova, Mark A Hink, Erik Limpens, Theodorus W J Gadella, Ton Bisseling
Rhizobial Nod factors are the key signaling molecules in the legume-rhizobium nodule symbiosis. In this study, the role of the Nod factor receptors NOD FACTOR PERCEPTION (NFP) and LYSIN MOTIF RECEPTOR-LIKE KINASE3 (LYK3) in establishing the symbiotic interface in root nodules was investigated. It was found that inside Medicago truncatula nodules, NFP and LYK3 localize at the cell periphery in a narrow zone of about two cell layers at the nodule apex. This restricted accumulation is narrower than the region of promoter activity/mRNA accumulation and might serve to prevent the induction of defense-like responses and/or to restrict the rhizobium release to precise cell layers...
October 2014: Plant Cell
Anna Pietraszewska-Bogiel, Benoit Lefebvre, Maria A Koini, Dörte Klaus-Heisen, Frank L W Takken, René Geurts, Julie V Cullimore, Theodorus W J Gadella
Receptor(-like) kinases with Lysin Motif (LysM) domains in their extracellular region play crucial roles during plant interactions with microorganisms; e.g. Arabidopsis thaliana CERK1 activates innate immunity upon perception of fungal chitin/chitooligosaccharides, whereas Medicago truncatula NFP and LYK3 mediate signalling upon perception of bacterial lipo-chitooligosaccharides, termed Nod factors, during the establishment of mutualism with nitrogen-fixing rhizobia. However, little is still known about the exact activation and signalling mechanisms of MtNFP and MtLYK3...
2013: PloS One
Thomas Rey, Amaury Nars, Maxime Bonhomme, Arnaud Bottin, Stéphanie Huguet, Sandrine Balzergue, Marie-Françoise Jardinaud, Jean-Jacques Bono, Julie Cullimore, Bernard Dumas, Clare Gough, Christophe Jacquet
Plant LysM proteins control the perception of microbial-derived N-acetylglucosamine compounds for the establishment of symbiosis or activation of plant immunity. This raises questions about how plants, and notably legumes, can differentiate friends and foes using similar molecular actors and whether any receptors can intervene in both symbiosis and resistance. To study this question, nfp and lyk3 LysM-receptor like kinase mutants of Medicago truncatula that are affected in the early steps of nodulation, were analysed following inoculation with Aphanomyces euteiches, a root oomycete...
April 2013: New Phytologist
Cara H Haney, Brendan K Riely, David M Tricoli, Doug R Cook, David W Ehrhardt, Sharon R Long
To form nitrogen-fixing symbioses, legume plants recognize a bacterial signal, Nod Factor (NF). The legume Medicago truncatula has two predicted NF receptors that direct separate downstream responses to its symbiont Sinorhizobium meliloti. NOD FACTOR PERCEPTION encodes a putative low-stringency receptor that is responsible for calcium spiking and transcriptional responses. LYSIN MOTIF RECEPTOR-LIKE KINASE3 (LYK3) encodes a putative high-stringency receptor that mediates bacterial infection. We localized green fluorescent protein (GFP)-tagged LYK3 in M...
July 2011: Plant Cell
Dörte Klaus-Heisen, Alessandra Nurisso, Anna Pietraszewska-Bogiel, Malick Mbengue, Sylvie Camut, Ton Timmers, Carole Pichereaux, Michel Rossignol, Theodorus W J Gadella, Anne Imberty, Benoit Lefebvre, Julie V Cullimore
Phylogenetic analysis has previously shown that plant receptor-like kinases (RLKs) are monophyletic with respect to the kinase domain and share an evolutionary origin with the animal interleukin-1 receptor-associated kinase/Pelle-soluble kinases. The lysin motif domain-containing receptor-like kinase-3 (LYK3) of the legume Medicago truncatula shows 33% amino acid sequence identity with human IRAK-4 over the kinase domain. Using the structure of this animal kinase as a template, homology modeling revealed that the plant RLK contains structural features particular to this group of kinases, including the tyrosine gatekeeper and the N-terminal extension α-helix B...
April 1, 2011: Journal of Biological Chemistry
Malick Mbengue, Sylvie Camut, Fernanda de Carvalho-Niebel, Laurent Deslandes, Solène Froidure, Dörte Klaus-Heisen, Sandra Moreau, Susana Rivas, Ton Timmers, Christine Hervé, Julie Cullimore, Benoit Lefebvre
LYK3 is a lysin motif receptor-like kinase of Medicago truncatula, which is essential for the establishment of the nitrogen-fixing, root nodule symbiosis with Sinorhizobium meliloti. LYK3 is a putative receptor of S. meliloti Nod factor signals, but little is known of how it is regulated and how it transduces these symbiotic signals. In a screen for LYK3-interacting proteins, we identified M. truncatula Plant U-box protein 1 (PUB1) as an interactor of the kinase domain. In planta, both proteins are localized and interact in the plasma membrane...
October 2010: Plant Cell
Vladimir Zhukov, Simona Radutoiu, Lene H Madsen, Tamara Rychagova, Evgenia Ovchinnikova, Alex Borisov, Igor Tikhonovich, Jens Stougaard
Phenotypic characterization of pea symbiotic mutants has provided a detailed description of the symbiosis with Rhizobium leguminosarum bv. viciae strains. We show here that two allelic non-nodulating pea mutants, RisNod4 and K24, are affected in the PsSym37 gene, encoding a LysM receptor kinase similar to Lotus japonicus NFR1 and Medicago truncatula LYK3. Phenotypic analysis of RisNod4 and K24 suggests a role for the SYM37 in regulation of infection-thread initiation and nodule development from cortical-cell division foci...
December 2008: Molecular Plant-microbe Interactions: MPMI
Patrick Smit, Erik Limpens, Rene Geurts, Elena Fedorova, Elena Dolgikh, Clare Gough, Ton Bisseling
Rhizobia secrete nodulation (Nod) factors, which set in motion the formation of nitrogen-fixing root nodules on legume host plants. Nod factors induce several cellular responses in root hair cells within minutes, but also are essential for the formation of infection threads by which rhizobia enter the root. Based on studies using bacterial mutants, a two-receptor model was proposed, a signaling receptor that induces early responses with low requirements toward Nod factor structure and an entry receptor that controls infection with more stringent demands...
September 2007: Plant Physiology
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