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Xiangning Bai, Sara Mernelius, Cecilia Jernberg, Ing-Marie Einemo, Stefan Monecke, Ralf Ehricht, Sture Löfgren, Andreas Matussek
Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhea (BD), hemorrhagic colitis (HC), and even hemolytic uremic syndrome (HUS). In Nordic countries, STEC are widely spread and usually associated with gastrointestinal symptoms and HUS. The objective of this study was to investigate the occurrence of STEC in Swedish patients over 10 years of age from 2003 through 2015, and to analyze the correlation of critical STEC virulence factors with clinical symptoms and duration of stx shedding. Diarrheal stool samples were screened for presence of stx by real-time PCR...
2018: Frontiers in Cellular and Infection Microbiology
David R Meier, Kristopher A Lofgren, Grzegorz T Gurda, Paraic A Kenny
Unlike humans, inbred genetically engineered mice have minimal inter-individual variation and, consequently, offer substantially increased statistical power for robust definition of recurrent cooperating cancer mutations. While technically feasible, whole exome sequencing is expensive and extremely data-intensive. Somatic mutation analysis using panels of 25-75 genes now provides detailed insight into the biology of human tumors. Here we report an adaptation for mouse tumors of a human PCR amplicon-based panel (Ion Torrent Cancer Hotspot Panel v2) allowing analysis of 18 cancer genes, including Kras, Nras, Hras, Pten, Pik3ca and Smad4, and encompassing regions homologous to more than 2000 known human cancer mutations...
May 9, 2018: Analytical Biochemistry
Maria Löfgren, Emilia Svala, Anders Lindahl, Eva Skiöldebrand, Stina Ekman
Osteoarthritis is an inflammatory and degenerative joint disease commonly affecting horses. To identify genes of relevance for cartilage pathology in osteoarthritis we studied the time-course effects of interleukin (IL)-1β on equine articular cartilage. Articular cartilage explants from the distal third metacarpal bone were collected postmortem from three horses without evidence of joint disease. The explants were stimulated with IL-1β for 27 days and global gene expression was measured by microarray. Gene expression was compared to that of unstimulated explants at days 3, 9, 15, 21 and 27...
May 1, 2018: Research in Veterinary Science
Robin Myte, Björn Gylling, Jenny Häggström, Jörn Schneede, Anna Löfgren-Burström, Jeroen R Huyghe, Göran Hallmans, Klaus Meyer, Ingegerd Johansson, Per Magne Ueland, Richard Palmqvist, Bethany Van Guelpen
Disturbances in one-carbon metabolism, intracellular reactions involved in nucleotide synthesis and methylation, likely increase the risk of colorectal cancer (CRC). However, results have been inconsistent. To explore whether this inconsistency could be explained by intertumoral heterogeneity, we evaluated a comprehensive panel of one-carbon metabolism biomarkers and some single nucleotide polymorphisms (SNPs) in relation to the risk of molecular subtypes of CRC defined by mutations in the KRAS and BRAF oncogenes...
2018: PloS One
Linda Rennie, Niklas Löfgren, Rolf Moe-Nilssen, Arve Opheim, Espen Dietrichs, Erika Franzén
BACKGROUND: Step-to-step variability is a marker of reduced motor control and a frequently studied outcome measure in neurodegenerative disorders such as Parkinson's disease (PD) as compared to healthy older adults (HOA). To challenge motor control of gait, walking should be tested at different gait speeds. Good reliability is essential, and gait variability estimates show good reproducibility when sampled at normal gait speed. The aim was therefore to investigate if gait variability could be reliably sampled at slow and fast speeds for individuals with PD and HOA by evaluating test-retest reliability...
April 14, 2018: Gait & Posture
Anette Larsson, Annie Palstam, Jan Bjersing, Monika Löfgren, Malin Ernberg, Eva Kosek, Björn Gerdle, Kaisa Mannerkorpi
BACKGROUND: Health and physical capacity are commonly associated with disease, age, and socioeconomic factors. The primary objective of this study was to investigate the degree to which physical capacity, defined as muscle strength and walking ability, is decreased in women with fibromyalgia (FM), as compared to healthy women, who are matched for age and level of education. The secondary aim was to investigate whether muscle strength and walking ability are associated with age, symptom duration, activity limitations and, Body Mass Index (BMI) in women with FM and control subjects...
April 19, 2018: BMC Musculoskeletal Disorders
Alexander Eiler, Anders Löfgren, Olle Hjerne, Sara Nordén, Peter Saetre
Detection of endangered species is invaluable for conservation efforts, yet many traditional sampling techniques are ineffective at low population abundances or during certain periods of the year. Here, we compared results from a newly developed eDNA approach and the traditional observational method for the endangered pool frog (Pelophylax lessonae). Analysis using an occupancy-modeling framework indicated that the probability of pools being occupied using eDNA (0.93) was higher than for the traditional method of counting calling males and silent observed individuals (0...
April 3, 2018: Scientific Reports
Sarah Thurston, Lisa Burlingame, Patrick A Lester, Jennifer Lofgren
New Zealand White (NZW) laboratory rabbits (Oryctolagus cuniculus), as well as their ancestors the European Rabbit, are a social species that exhibit numerous benefits to being housed accordingly. Although these rabbits are innately gregarious, certain behaviors can still arise when kept in captivity, which if left unchecked, can confound research results or lead to wounding, which in extreme cases can be severe. To prevent these issues, there must be a well-structured plan for the monitoring and maintenance of paired laboratory rabbits...
March 16, 2018: Journal of Visualized Experiments: JoVE
K C Chartkunchand, M H Stockett, E K Anderson, G Eklund, M K Kristiansson, M Kamińska, N de Ruette, M Blom, M Björkhage, A Källberg, P Löfgren, P Reinhed, S Rosén, A Simonsson, H Zettergren, H T Schmidt, H Cederquist
A sputter ion source with a solid graphite target has been used to produce dianions with a focus on carbon cluster dianions, Cn 2- , with n = 7-24. Singly and doubly charged anions from the source were accelerated together to kinetic energies of 10 keV per atomic unit of charge and injected into one of the cryogenic (13 K) ion-beam storage rings of the Double ElectroStatic Ion Ring Experiment facility at Stockholm University. Spontaneous decay of internally hot Cn 2- dianions injected into the ring yielded Cn - anions with kinetic energies of 20 keV, which were counted with a microchannel plate detector...
March 2018: Review of Scientific Instruments
Andreas Kronbichler, Anna K Luger, Lorin Loacker, Alex Pizzini, Judith Löffler-Ragg, Ines Peschel
No abstract text is available yet for this article.
March 21, 2018: Clinical and Experimental Rheumatology
Mika Nieminen, Sirpa Piirainen, Ulf Sikström, Stefan Löfgren, Hannu Marttila, Sakari Sarkkola, Ari Laurén, Leena Finér
The objective of this study was to evaluate the potential of different water management options to mitigate sediment and nutrient exports from ditch network maintenance (DNM) areas in boreal peatland forests. Available literature was reviewed, past data reanalyzed, effects of drainage intensity modeled, and major research gaps identified. The results indicate that excess downstream loads may be difficult to prevent. Water protection structures constructed to capture eroded matter are either inefficient (sedimentation ponds) or difficult to apply (wetland buffers)...
March 27, 2018: Ambio
Aurelie Tomczak, Jonathan M Mortensen, Rainer Winnenburg, Charles Liu, Dominique T Alessi, Varsha Swamy, Francesco Vallania, Shane Lofgren, Winston Haynes, Nigam H Shah, Mark A Musen, Purvesh Khatri
Gene Ontology (GO) enrichment analysis is ubiquitously used for interpreting high throughput molecular data and generating hypotheses about underlying biological phenomena of experiments. However, the two building blocks of this analysis - the ontology and the annotations - evolve rapidly. We used gene signatures derived from 104 disease analyses to systematically evaluate how enrichment analysis results were affected by evolution of the GO over a decade. We found low consistency between enrichment analyses results obtained with early and more recent GO versions...
March 23, 2018: Scientific Reports
Vanessa L Oliver, Sarah E Thurston, Jennifer L Lofgren
Recent studies have revealed some of the most frequently used analgesics in mice are not effectively treating postoperative pain. Our laboratory sought to compare and assess the validity and reliability of 2 cageside pain assessments that we recently developed for use in mice-nesting consolidation and grooming transfer tests. We then applied these tests to compare the efficacy of commonly used analgesics-buprenorphine (0.1 mg/kg SC every 12 h for 48 h) and carprofen (30 mg/kg in drinking water for 72 h)-alone and in multimodal combination as a refinement for treating postoperative pain in mice...
March 1, 2018: Journal of the American Association for Laboratory Animal Science: JAALAS
Jessica Cohen, Katherine Lofgren, Margaret McConnell
No abstract text is available yet for this article.
May 2017: American Economic Review
Monika Löfgren, Christina H Opava, Ingrid Demmelmaier, Cecilia Fridén, Ingrid E Lundberg, Birgitta Nordgren, Eva Kosek
BACKGROUND: We aimed to explore pressure pain sensitivity and the function of segmental and plurisegmental exercise-induced hypoalgesia (EIH) in persons with rheumatoid arthritis (RA) compared with healthy control subjects (HC). METHODS: Forty-six participants with RA (43 female, 3 male) and 20 HC (16 female, 4 male) participated in the study. Pressure pain thresholds, suprathreshold pressure pain at rest, and segmental and plurisegmental EIH during standardised submaximal contractions were assessed by algometry...
March 15, 2018: Arthritis Research & Therapy
Amelia E Van Pelt, Beatriz Quiñones, Hannah L Lofgren, Faith E Bartz, Kira L Newman, Juan S Leon
Foodborne illness burdens individuals around the world and may be caused by consuming fresh produce contaminated with bacterial, parasite, and viral pathogens. Pathogen contamination on produce may originate at the farm and packing facility. This research aimed to determine the prevalence of human pathogens (bacteria, parasites, and viruses) on fresh produce (fruits, herbs, and vegetables) on farms and in packing facilities worldwide through a systematic review of 38 peer-reviewed articles. The median and range of the prevalence was calculated, and Kruskal-Wallis tests and logistic regression were performed to compare prevalence among pooled samples of produce groups, pathogen types, and sampling locations...
2018: Frontiers in Public Health
Elizabeth Nalintya, David B Meya, Sarah Lofgren, Kathy Huppler Hullsiek, David R Boulware, Radha Rajasingham
BACKGROUND: Cryptococcus is a leading cause of AIDS-related mortality. Cryptococcal antigen (CrAg) is detectable in blood before meningitis onset and predicts death. CrAg screening among those with advanced HIV, and treatment of those CrAg+ with fluconazole, has demonstrated survival benefit. However, implementation and widespread uptake have been slow outside clinical trials. METHODS: We designed a CrAg screening program for routine care that incorporated intensive education and training of clinic staff...
June 1, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Mia Fahlén, Hua Zhang, Lars Löfgren, Britt Masironi, Eva VON Schoultz, B O VON Schoultz, Lena Sahlin
BACKGROUND: Progesterone and androgens are important for normal development and tumorigenesis of the breast. PATIENTS AND METHODS: Breast tissue samples from 49 premenopausal women were obtained. The progesterone receptors (PRA, PRB, PGRMC1 and PGRMC2) and the androgen receptor (AR) were determined in malignant and benign breast tumors and control tissues. RESULTS: The PRB and AR mRNA levels were highest in tumors. PGRMC1 and PGRMC2 mRNA levels were higher in malignant tumors compared to their paired normal tissues...
March 2018: Anticancer Research
Lars W Andersen, Mathias J Holmberg, Asger Granfeldt, Bo Løfgren, Kimberly Vellano, Bryan F McNally, Bob Siegerink, Tobias Kurth, Michael W Donnino
BACKGROUND: Automated external defibrillators (AEDs) can be used by bystanders to provide rapid defibrillation for patients with out-of-hospital cardiac arrest (OHCA). Whether neighborhood characteristics are associated with AED use is unknown. Furthermore, the association between AED use and outcomes has not been well characterized for all (i.e. shockable and non-shockable) public OHCAs. METHODS: We included public, non-911-responder witnessed OHCAs registered in the Cardiac Arrest Registry to Enhance Survival (CARES) between 2013 and 2016...
May 2018: Resuscitation
Marie Vognstoft Hjortbak, Johanne Hjort, Jonas Agerlund Povlsen, Rebekka Vibjerg Jensen, Nicolaj Brejnholdt Støttrup, Mia R Laursen, Nichlas Riise Jespersen, Bo Løfgren, Hans Erik Bøtker
Augmented mortality and morbidity following an acute myocardial infarction in patients with diabetes mellitus Type 2 (T2DM) may be caused by increased sensitivity to ischemia reperfusion (IR) injury or altered activation of endogenous cardioprotective pathways modified by T2DM per se or ischemic preconditioning (IPC). We aimed to investigate, whether the duration of T2DM influences sensitivity against IR injury and the efficacy of IPC, and how myocardial glucose oxidation rate was involved. Male Zucker diabetic fatty rats (homozygote (fa/fa)) at ages 6-(prediabetic), 12- (onset diabetes) and 24-weeks of age (late diabetes) and their age-matched non-diabetic controls (heterozygote (fa/+) were subjected to IR injury in the Langendorff model and randomised to IPC stimulus or control...
2018: PloS One
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