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https://www.readbyqxmd.com/read/28107862/neuroprotective-effect-of-spilanthes-acmella-murr-on-pesticide-induced-neuronal-cells-death
#1
Wilasinee Suwanjang, Bongkot Khongniam, Sujittra Srisung, Supaluk Prachayasittikul, Virapong Prachayasittikul
OBJECTIVE: To investigate protective effects of Spilanthes acmella (S. acmella) Murr. extracts against pesticide-induced neuronal cells death and to elucidate the underlying molecular mechanism in dopaminergic (SH-SY5Y) cells lines. METHODS: Cell viability of SH-SY5Y cells was studied by treating the cells with various concentration of pirimicarb for 24 h. Neuroprotective effect of S. acmella Murr. extracts was investigated by adding the plant extracts to the medium for 24 h prior to the incubation with 100 μM H2O2 or with pirimicarb for 24 h...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28018909/a-review-of-selected-genes-with-known-effects-on-performance-and-health-of-cattle
#2
REVIEW
Eduardo Casas, Marcus E Kehrli
There are genetic conditions that influence production in dairy and beef cattle. The objective of this review was to describe relevant genetic conditions that have been associated with productivity and health in cattle. Genes or genomic regions that have been identified as a candidate for the condition will be included, and the genetic basis of the condition will be defined. Genes and genetic conditions included in this review are bovine leukocyte adhesion deficiency, deficiency of the uridine monophosphate synthase, bovine chronic interstitial nephritis, horn development, myostatin, complex vertebral malformation, leptin, osteopetrosis, apoptosis peptide activating factor 1, chondrodysplastic dwarfism, caseins, calpastatin, umbilical hernia, lactoglobulin, citrullinemia, cholesterol deficiency, prions, thyroglobulin, diacylglycerol acyltransferase, syndactyly, maple syrup urine disease, slick hair, Factor XI deficiency, and μ-Calpain...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28013348/calpain-1-cleaves-and-inactivates-prostacyclin-synthase-in-mesenteric-arteries-from-diabetic-mice
#3
Voahanginirina Randriamboavonjy, Anastasia Kyselova, Amro Elgheznawy, Sven Zukunft, Ilka Wittig, Ingrid Fleming
Diabetes is associated with a number of co-morbidities including an increased risk of developing cardiovascular diseases. The activation of Ca(2+)-activated proteases of the calpain family has been implicated in platelet activation associated with diabetes and this study aimed to determine the role of calpain activation in the development of endothelial dysfunction. Diabetes induction in mice attenuated acetylcholine-induced relaxation of mesenteric artery rings, an effect prevented in mice receiving a calpain inhibitor...
January 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/27956030/calpain-1-resident-in-lipid-raft-caveolin-1-membrane-microdomains-plays-a-protective-role-in-endothelial-cells
#4
Antonino Martines, Roberto Stifanese, Emanuela L Faelli, Luisa Perasso, Ilaria Melloni, Piero Ruggeri, Monica Averna
We are here reporting that calpain-1 is a constitutive component of a distinct lipid raft/caveolin-1 microdomain isolated from bEnd5 cells in association with endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90). Perturbations in intracellular calcium concentration by Ca(2+)-ionophore A23187 or prolonged cell exposure to high glucose induce a significant decrease in the level of eNOS accompanied by a recruitment of additional HSP90 molecules at this site. In these conditions the cells are more resistant to cell death by Ca(2+) overload...
February 2017: Biochimie
https://www.readbyqxmd.com/read/27875293/attenuation-of-blood-brain-barrier-breakdown-and-hyperpermeability-by-calpain-inhibition
#5
Himakarnika Alluri, Marcene Grimsley, Chinchusha Anasooya Shaji, Kevin Paul Varghese, Shenyuan L Zhang, Chander Peddaboina, Bobby Robinson, Madhava R Beeram, Jason H Huang, Binu Tharakan
Blood-brain barrier (BBB) breakdown and the associated microvascular hyperpermeability followed by brain edema are hallmark features of several brain pathologies, including traumatic brain injuries (TBI). Recent studies indicate that pro-inflammatory cytokine interleukin-1β (IL-1β) that is up-regulated following traumatic injuries also promotes BBB dysfunction and hyperpermeability, but the underlying mechanisms are not clearly known. The objective of this study was to determine the role of calpains in mediating BBB dysfunction and hyperpermeability and to test the effect of calpain inhibition on the BBB following traumatic insults to the brain...
December 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27835610/roles-of-calpain-calpastatin-system-ccs-in-human-t-cell-activation
#6
Anna Mikosik, Aleksandra Jasiulewicz, Agnieszka Daca, Izabella Henc, Joanna E Frąckowiak, Katarzyna Ruckemann-Dziurdzińska, Jerzy Foerster, Aurelie Le Page, Ewa Bryl, Tamas Fulop, Jacek M Witkowski
The immune response is determined by the speed of the T cell reaction to antigens assured by a state of readiness for proliferation and cytokine secretion. Proliferation, apoptosis and motion of many cell types are controlled by cytoplasmic proteases - µ- and m-calpain - and their inhibitor calpastatin, together forming the "calpain-calpastatin system" (CCS), assumed to modify their targets only upon activation-dependent cytoplasmic Ca2+ increase. Contrastingly to this notion, using quantitative real time PCR and semiquantitative flow cytometry respectively, we show here that the CCS genes are constitutively expressed, and that both calpains are constitutively active in resting, circulating human CD4+ and CD8+ lymphocytes...
November 9, 2016: Oncotarget
https://www.readbyqxmd.com/read/27812546/%C3%AE-cell-specific-increased-expression-of-calpastatin-prevents-diabetes-induced-by-islet-amyloid-polypeptide-toxicity
#7
Tatyana Gurlo, Safia Costes, Jonathan D Hoang, Jacqueline F Rivera, Alexandra E Butler, Peter C Butler
The islet in type 2 diabetes (T2D) shares many features of the brain in protein misfolding diseases. There is a deficit of β cells with islet amyloid derived from islet amyloid polypeptide (IAPP), a protein coexpressed with insulin. Small intracellular membrane-permeant oligomers, the most toxic form of IAPP, are more frequent in β cells of patients with T2D and rodents expressing human IAPP. β Cells in T2D, and affected cells in neurodegenerative diseases, share a comparable pattern of molecular pathology, including endoplasmic reticulum stress, mitochondrial dysfunction, attenuation of autophagy, and calpain hyperactivation...
November 3, 2016: JCI Insight
https://www.readbyqxmd.com/read/27765842/adrenodemedullation-activates-the-ca2-dependent-proteolysis-in-soleus-muscles-from-rats-exposed-to-cold
#8
Leandro H Manfredi, D Lustrino, Juliano Machado, Wilian Assis Silveira, Neusa Maria Zanon, Luiz C Navegantes, Isis do Carmo Kettelhut
Previous studies have shown that catecholamines in vivo and in vitro inhibit the activity of Ca(2+)-dependent proteolysis in skeletal muscles under basal conditions. In the present study we sought to investigate the role of catecholamines in regulating the Ca(2+)-dependent proteolysis in soleus and extensor digitorum longus (EDL) muscles from rats acutely exposed to cold. Overall proteolysis, the activity of proteolytic systems as well as protein levels and gene expression of different components of calpain system were investigated in rats submitted to adrenodemedullation (ADMX) and exposed to cold for 24 h...
October 20, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27747292/role-of-calpain-in-pathogenesis-of-human-disease-processes
#9
Brittany A Potz, M Ruhul Abid, Frank W Sellke
Calpains are a 15-member class of calcium activated nonlysosomal neutral proteases which are involved in a broad range of cellular function. Calpains are usually localized to the cytosol and within mitochondria. Calpastatin is an endogenous protein that specifically binds to and inhibits calpain. Overactivation of calpain has been implicated in a number of disease processes of the brain, eyes, heart, lungs, pancreas, kidneys, vascular system and skeletal muscle. Therefore, calpain may serve as a potential therapeutic target for a wide variety of disease processes...
2016: Journal of Nature and Science
https://www.readbyqxmd.com/read/27715410/kshv-reduces-autophagy-in-thp-1-cells-and-in-differentiating-monocytes-by-decreasing-cast-calpastatin-and-atg5-expression
#10
R Santarelli, M Granato, G Pentassuglia, V Lacconi, M S Gilardini Montani, R Gonnella, M Tafani, M R Torrisi, A Faggioni, M Cirone
We have previously shown that Kaposi sarcoma-associated herpesvirus (KSHV) impairs monocyte differentiation into dendritic cells (DCs). Macroautophagy/autophagy has been reported to be essential in such a differentiating process. Here we extended these studies and found that the impairment of DC formation by KSHV occurs through autophagy inhibition. KSHV indeed reduces CAST (calpastatin) and consequently decreases ATG5 expression in both THP-1 monocytoid cells and primary monocytes. We unveiled a new mechanism put in place by KSHV to escape from immune control...
December 2016: Autophagy
https://www.readbyqxmd.com/read/27635352/effect-of-8-hydroxyquinoline-and-derivatives-on-human-neuroblastoma-sh-sy5y-cells-under-high-glucose
#11
Wilasinee Suwanjang, Supaluk Prachayasittikul, Virapong Prachayasittikul
8-Hydroxyquinoline and derivatives exhibit multifunctional properties, including antioxidant, antineurodegenerative, anticancer, anti-inflammatory and antidiabetic activities. In biological systems, elevation of intracellular calcium can cause calpain activation, leading to cell death. Here, the effect of 8-hydroxyquinoline and derivatives (5-chloro-7-iodo-8-hydroxyquinoline or clioquinol and 8-hydroxy-5-nitroquinoline or nitroxoline) on calpain-dependent (calpain-calpastatin) pathways in human neuroblastoma (SH-SY5Y) cells was investigated...
2016: PeerJ
https://www.readbyqxmd.com/read/27472894/dual-roles-of-calpain-in-facilitating-coxsackievirus-b3-replication-and-prompting-inflammation-in-acute-myocarditis
#12
Minghui Li, Yangang Su, Yong Yu, Ying Yu, Xinggang Wang, Yunzeng Zou, Junbo Ge, Ruizhen Chen
BACKGROUND: Viral myocarditis (VMC) treatment has long been lacking of effective methods. Our former studies indicated roles of calpain in VMC pathogenesis. This study aimed at verifying the potential of calpain in Coxsackievirus B3 (CVB3)-induced myocarditis treatment. METHODS: A transgenic mouse overexpressing the endogenous calpain inhibitor, calpastatin, was introduced in the study. VMC mouse model was established via intraperitoneal injection of CVB3 in transgenic and wild mouse respectively...
October 15, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27456359/gene-protein-expression-of-capn1-2-cast-system-members-is-associated-with-erk1-2-kinases-activity-as-well-as-progression-and-clinical-outcome-in-human-laryngeal-cancer
#13
Katarzyna Starska, Ewa Forma, Paweł Jóźwiak, Iwona Lewy-Trenda, Marian Danilewicz, Olga Stasikowska-Kanicka, Michał Skóra, Katarzyna Kolary, Jakub Miazga, Anna Krześlak, Magdalena Bryś
Recent evidence indicates the involvement of calpains (CAPNs), a family of cysteine proteases, in cancer development and progression, as well as the insufficient response to cancer therapies. The contribution of CAPNs and regulatory calpastatin (CAST) and ERK1/2 kinases to aggressiveness, disease course, and outcome in laryngeal cancer remains elusive. This study was aimed to evaluate the CAPN1/2-CAST-ERK1/2 enzyme system mRNA/protein level and to investigate whether they can promote the dynamic of tumor growth and prognosis...
October 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27453531/the-inhibition-of-calpains-ameliorates-vascular-restenosis-through-mmp2-tgf-%C3%AE-1-pathway
#14
Lianghu Tang, Haifeng Pei, Yi Yang, Xiong Wang, Ting Wang, Erhe Gao, De Li, Yongjian Yang, Dachun Yang
Restenosis limits the efficacy of vascular percutaneous intervention, in which vascular smooth muscle cell (VSMC) proliferation and activation of inflammation are two primary causal factors. Calpains influence VSMC proliferation and collagen synthesis. However, the roles of calpastatin and calpains in vascular restenosis remain unclear. Here, restenosis was induced by ligating the left carotid artery, and VSMCs were pretreated with platelet-derived growth factor (PDGF)-BB. Adenovirus vector carrying MMP2 sequence and specific small interfering RNA against calpain-1/2 were introduced...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27453331/calpastatin-overexpression-reduces-oxidative-stress-induced-mitochondrial-impairment-and-cell-death-in-human-neuroblastoma-sh-sy5y-cells-by-decreasing-calpain-and-calcineurin-activation-induction-of-mitochondrial-fission-and-destruction-of-mitochondrial-fusion
#15
Kulvadee Tangmansakulchai, Zuroida Abubakar, Narisorn Kitiyanant, Wilasinee Suwanjang, Chaniya Leepiyasakulchai, Piyarat Govitrapong, Banthit Chetsawang
Calpain is an intracellular Ca(2+)-dependent protease, and the activation of calpain has been implicated in neurodegenerative diseases. Calpain activity can be regulated by calpastatin, an endogenous specific calpain inhibitor. Several lines of evidence have demonstrated a potential role of calpastatin in preventing calpain-mediated pathogenesis. Additionally, several studies have revealed that calpain activation and mitochondrial damage are involved in the cell death process; however, recent evidence has not clearly indicated a neuroprotective mechanism of calpastatin against calpain-dependent mitochondrial impairment in the process of neuronal cell death...
September 2016: Mitochondrion
https://www.readbyqxmd.com/read/27427783/zilpaterol-hydrochloride-improves-beef-yield-changes-palatability-traits-and-increases-calpain-calpastatin-gene-expression-in-nellore-heifers
#16
Nara Regina Brandão Cônsolo, Viviane Borba Ferrari, Ligia Garcia Mesquita, Rodrigo Silva Goulart, Luis Felipe Prada E Silva
This research aimed to evaluate the effects of the beta-agonist zilpaterol hydrochloride (ZH) on carcass traits, subprimal yield, meat quality, palatability traits, and gene expression in Nellore heifers. Zilpaterol increased Longissimus lumborum area and did not change back fat thickness, meat color, and cooking loss. Heifers fed ZH had greater hindquarter weight and carcass percentage. Muscles from hindquarter were heavier for animals fed ZH. Forequarter (% of carcass) decreased and brisket did not change with ZH supplementation...
November 2016: Meat Science
https://www.readbyqxmd.com/read/27420951/single-nucleotide-polymorphisms-associated-with-carcass-traits-in-a-population-of-brahman-and-brahman-influenced-steers
#17
A M Royer, C Shivers, D G Riley, M A Elzo, M D Garcia
Brahman cattle are important in tropical regions due to their ability to tolerate excessive heat and parasites. However, Brahman cattle exhibit lower carcass quality characteristics when compared to Bos taurus breeds. The objective of this study was to evaluate potential associations between single nucleotide polymorphisms (SNPs) in six candidate genes for carcass quality and composition traits in a population of Brahman and Brahman-influenced steers. Steers were evaluated through the American Brahman Breeders Association carcass evaluation project in Gonzales, Texas...
June 21, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27323818/the-calpain-system-is-associated-with-survival-of-breast-cancer-patients-with-large-but-operable-inflammatory-and-non-inflammatory-tumours-treated-with-neoadjuvant-chemotherapy
#18
Sarah J Storr, Siwei Zhang, Tim Perren, Mark Lansdown, Hiba Fatayer, Nisha Sharma, Renu Gahlaut, Abeer Shaaban, Stewart G Martin
The calpains are a family of intracellular cysteine proteases that function in a variety of important cellular functions, including cell signalling, motility, apoptosis and survival. In early invasive breast cancer expression of calpain-1, calpain-2 and their inhibitor, calpastatin, have been associated with clinical outcome and clinicopathological factors.The expression of calpain-1, calpain-2 and calpastatin was determined using immunohistochemistry on core biopsy samples, in a cohort of large but operable inflammatory and non-inflammatory primary breast cancer patients treated with neoadjuvant chemotherapy...
July 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27294874/a-calcium-and-calpain-dependent-pathway-determines-the-response-to-lenalidomide-in-myelodysplastic-syndromes
#19
Jing Fang, Xiaona Liu, Lyndsey Bolanos, Brenden Barker, Carmela Rigolino, Agostino Cortelezzi, Esther N Oliva, Maria Cuzzola, H Leighton Grimes, Celia Fontanillo, Kakajan Komurov, Kyle MacBeth, Daniel T Starczynowski
Despite the high response rates of individuals with myelodysplastic syndrome (MDS) with deletion of chromosome 5q (del(5q)) to treatment with lenalidomide (LEN) and the recent identification of cereblon (CRBN) as the molecular target of LEN, the cellular mechanism by which LEN eliminates MDS clones remains elusive. Here we performed an RNA interference screen to delineate gene regulatory networks that mediate LEN responsiveness in an MDS cell line, MDSL. We identified GPR68, which encodes a G-protein-coupled receptor that has been implicated in calcium metabolism, as the top candidate gene for modulating sensitivity to LEN...
July 2016: Nature Medicine
https://www.readbyqxmd.com/read/27158675/eosinophilic-esophagitis-linked-calpain-14-is-an-il-13-induced-protease-that-mediates-esophageal-epithelial-barrier-impairment
#20
Benjamin P Davis, Emily M Stucke, M Eyad Khorki, Vladislav A Litosh, Jeffrey K Rymer, Mark Rochman, Jared Travers, Leah C Kottyan, Marc E Rothenberg
We recently identified a genome-wide genetic association of eosinophilic esophagitis (EoE) at 2p23 spanning the calpain 14 (CAPN14) gene, yet the causal mechanism has not been elucidated. We now show that recombinant CAPN14 cleaves a calpain-specific substrate and is inhibited by 4 classical calpain inhibitors: MDL-28170, acetyl-calpastatin, E-64, and PD151746. CAPN14 is specifically induced (>100-fold) in esophageal epithelium after IL-13 treatment. Epithelial cells overexpressing CAPN14 display impaired epithelial architecture, characterized by acantholysis, epidermal clefting, and epidermolysis...
April 2016: JCI Insight
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