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agrobacterium rhizogenes

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https://www.readbyqxmd.com/read/29760765/an-improved-method-for-agrobacterium-rhizogenes-mediated-transformation-of-tomato-suitable-for-the-study-of-arbuscular-mycorrhizal-symbiosis
#1
Tania Ho-Plágaro, Raúl Huertas, María I Tamayo-Navarrete, Juan A Ocampo, José M García-Garrido
Background: Solanum lycopersicum , an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a method to easily screen candidate genes which could play an important role during tomato mycorrhization is required. Results: We have developed an optimized procedure for composite tomato plant obtaining achieved through Agrobacterium rhizogenes -mediated transformation...
2018: Plant Methods
https://www.readbyqxmd.com/read/29687371/metabolic-engineering-of-glycyrrhizin-pathway-by-over-expression-of-beta-amyrin-11-oxidase-in-transgenic-roots-of-glycyrrhiza-glabra
#2
Zahra Shirazi, Ali Aalami, Masoud Tohidfar, Mohammad Mehdi Sohani
Glycyrrhiza glabra is one of the most important and well-known medicinal plants which produces various triterpene saponins such as glycyrrhizin. Beta-amyrin 11-oxidase (CYP88D6) plays a key role in engineering pathway of glycyrrhizin production and converts an intermediated beta-amyrin compound to glycyrrhizin. In this study, pBI121GUS-9 :CYP88D6 construct was transferred to G. glabra using Agrobacterium rhizogene ATCC 15834. The quantitation of transgene was measured in putative transgenic hairy roots using qRT-PCR...
April 23, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29665737/increased-synthesis-of-a-new-oleanane-type-saponin-in-hairy-roots-of-marigold-calendula-officinalis-after-treatment-with-jasmonic-acid
#3
Michał Markowski, Marek Długosz, Anna Szakiel, Mathieu Durli, Sophie Poinsignon, Sabine Bouguet-Bonnet, Lionel Vernex-Loset, Gabriel Krier, Max Henry
Native plant of marigold (Calendula officinalis L.) synthesizes oleanolic acid saponins classified as glucosides or glucuronides according to the first residue in sugar chain bound to C-3 hydroxyl group. Hairy root culture, obtained by transformation with Agrobacterium rhizogenes strain 15834, exhibit a potent ability of synthesis of oleanolic acid glycosides. The HPLC profile of saponin fraction obtained from C. officinalis hairy roots treated with plant stress hormone, jasmonic acid, showed the 10-times increase of the content of one particular compound, determined by NMR and MALDI TOF as a new bisdesmoside saponin, 3-O-β-d-glucuronopyranosyl-28-O-β-d-galactopyranosyl-oleanolic acid...
April 18, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29656128/agrobacterium-rhizogenes-mediated-transformation-of-a-dioecious-plant-model-silene-latifolia
#4
Vojtech Hudzieczek, Radim Cegan, Tomas Cermak, Nela Bacovska, Zuzana Machalkova, Karel Dolezal, Lucie Plihalova, Daniel Voytas, Roman Hobza, Boris Vyskot
Silene latifolia serves as a model species to study dioecy, the evolution of sex chromosomes, dosage compensation and sex-determination systems in plants. Currently, no protocol for genetic transformation is available for this species, mainly because S. latifolia is considered recalcitrant to in vitro regeneration and infection with Agrobacterium tumefaciens. Using cytokinins and their synthetic derivatives, we markedly improved the efficiency of regeneration. Several agrobacterial strains were tested for their ability to deliver DNA into S...
April 12, 2018: New Biotechnology
https://www.readbyqxmd.com/read/29650153/increasing-genetic-variability-in-oilseed-rape-brassica-napus-genotypes-and-phenotypes-of-oilseed-rape-transformed-by-wild-type-agrobacterium-rhizogenes
#5
Josefine Nymark Hegelund, Chang Liang, Uffe Bjerre Lauridsen, Oliver Kemp, Henrik Lütken, Renate Müller
Brassica napus (oilseed rape) is a major oil crop worldwide. Due to the short domestication period of oilseed rape the genetic variability is limited compared to other crops. Transfer of rol and aux genes from Agrobacterium rhizogenes is used in horticulture to increase genetic variability. In the current study, we explore transformation by A. rhizogenes as a biotechnological approach in breeding for more branched and shorter oilseed rape. In the 2nd generation of transformed oilseed rape, branch numbers increased significantly by 49% from 7...
June 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29587452/investigation-of-linum-flavum-l-hairy-root-cultures-for-the-production-of-anticancer-aryltetralin-lignans
#6
Sullivan Renouard, Cyrielle Corbin, Samantha Drouet, Barbara Medvedec, Joël Doussot, Cyril Colas, Benoit Maunit, Avninder S Bhambra, Eric Gontier, Nathalie Jullian, François Mesnard, Michèle Boitel, Bilal Haider Abbasi, Randolph R J Arroo, Eric Lainé, Christophe Hano
Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated...
March 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29500560/agrobacterium-mediated-transformation-in-the-evolution-of-plants
#7
Tatiana V Matveeva
In most cases, the genetic engineering of plants uses Agrobacterium-mediated transformation to introduce novel genes. In nature, insertion of T-DNA into the plant genome and its subsequent transfer via sexual reproduction have been shown for several species in the genera Nicotiana, Ipomoea , and Linaria . A sequence homologous to T-DNA of the Ri plasmid of Agrobacterium rhizogenes was found in the genome of wild-type Nicotiana glauca (section Noctiflorae) more than 30 years ago and was named "cellular T-DNA" (cT-DNA)...
March 3, 2018: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/29483937/protocol-high-efficiency-in-planta-agrobacterium-mediated-transgenic-hairy-root-induction-of-camellia-sinensis-var-sinensis
#8
Karthikeyan Alagarsamy, Lubobi Ferdinand Shamala, Shu Wei
Background: Camellia sinensis var. sinensis is widely grown for tea beverages that possess significant health promoting effects. Studies on tea plant genetics and breeding are hindered due to its recalcitrance to Agrobacterium -mediated genetic transformation. Among the possible reasons, oxidation of phenolics released from explant tissues and bactericidal effects of tea polyphenols during the process of transformation play a role in the plant recalcitrance. The aim of the present study was to alleviate the harmful effects of phenolic compounds using in - planta transformation...
2018: Plant Methods
https://www.readbyqxmd.com/read/29460196/a-comparative-study-of-biotechnological-approaches-for-producing-valuable-flavonoids-in-prosopis-farcta
#9
Somaieh Zafari, Mohsen Sharifi, Najmeh Ahmadian Chashmi
The callus and hairy root cultures of Prosopis farcta were established to develop effective strategies to enhance its valuable and medicinally important flavonoid compounds. For callus induction, the hypocotyl, cotyledon and shoot explants were subjected to different plant hormones, naphthalene acetic acid (NAA), benzylaminopurine (BAP), kinetin and dichlorophenoxyacetic acid (2,4-D). Greater callus induction was obtained from hypocotyl explants on MS medium containing 3.0 mg L-1 NAA + 2.0 mg L-1 BAP...
April 2018: Cytotechnology
https://www.readbyqxmd.com/read/29450118/elite-hairy-roots-of-raphanus-sativus-l-as-a-source-of-antioxidants-and-flavonoids
#10
Muthusamy Balasubramanian, Murugesan Anbumegala, Ramasamy Surendran, Muthukrishnan Arun, Girija Shanmugam
An efficient protocol for hairy root induction in radish was established by optimizing several parameters that affect the efficiency of Agrobacterium rhizogenes -mediated transformations. Explants wounded using sterile hypodermic needle, infected with Agrobacterium suspension (0.6 OD600 ) for 10 min and co-cultivated in 1/2 MS medium containing acetosyringone (100 µM) for 2 days displayed maximum percentage of hairy root induction using MTCC 2364 (77.6%) and MTCC 532 (67.6%). On further experiments with MTCC 2364 initiated hairy roots, maximum biomass accumulation (fresh weight = 9...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29417086/biotic-elicitation-for-scopolamine-production-by-hairy-root-cultures-of-datura-metel
#11
Zahra Shakeran, Mehrnaz Keyhanfar, Mustafa Ghanadian
The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Datura metel was used as an advantageous method for production of scopolamine. The hairy roots are formed by Agrobacterium rhizogenes and have genetic stability, high growth rate and lateral branching...
December 2017: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/29396465/the-rolb-plant-oncogene-affects-multiple-signaling-protein-modules-related-to-hormone-signaling-and-plant-defense
#12
Victor P Bulgakov, Yulia V Vereshchagina, Dmitry V Bulgakov, Galina N Veremeichik, Yuri N Shkryl
The rolB plant oncogene of Agrobacterium rhizogenes perturbs many biochemical processes in transformed plant cells, thereby causing their neoplastic reprogramming. The oncogene renders the cells more tolerant to environmental stresses and herbicides and inhibits ROS elevation and programmed cell death. In the present work, we performed a proteomic analysis of Arabidopsis thaliana rolB-expressing callus line AtB-2, which represents a line with moderate expression of the oncogene. Our results show that under these conditions rolB greatly perturbs the expression of some chaperone-type proteins such as heat-shock proteins and cyclophilins...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29364203/plant-promoter-analysis-identification-and-characterization-of-root-nodule-specific-promoter-in-the-common-bean
#13
Kalpana Nanjareddy, Manoj-Kumar Arthikala, Alma-Leticia Aguirre, Brenda-Mariana Gómez, Miguel Lara
The upstream sequences of gene coding sequences are termed as promoter sequences. Studying the expression patterns of promoters are very significant in understanding the gene regulation and spatiotemporal expression patterns of target genes. On the other hand, it is also critical to establish promoter evaluation tools and genetic transformation techniques that are fast, efficient, and reproducible. In this study, we investigated the spatiotemporal expression pattern of the rhizobial symbiosis-specific nodule inception (NIN) promoter of Phaseolus vulgaris in the transgenic hairy roots...
December 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29329601/nicotiana-glauca-whole-genome-investigation-for-ct-dna-study
#14
Galina Khafizova, Pavel Dobrynin, Dmitrii Polev, Tatiana Matveeva
OBJECTIVE: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. DATA DESCRIPTION: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion...
January 12, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29287271/maize-root-culture-as-a-model-system-for-studying-azoxystrobin-biotransformation-in-plants
#15
Maheswor Gautam, Mohamed Elhiti, Inge S Fomsgaard
Hairy roots induced by Agrobacterium rhizogenes are well established models to study the metabolism of xenobiotics in plants for phytoremediation purposes. However, the model requires special skills and resources for growing and is a time-consuming process. The roots induction process alters the genetic construct of a plant and is known to express genes that are normally absent from the non-transgenic plants. In this study, we propose and establish a non-transgenic maize root model to study xenobiotic metabolism in plants for phytoremediation purpose using azoxystrobin as a xenobiotic compound...
March 2018: Chemosphere
https://www.readbyqxmd.com/read/29278928/exploring-plant-tissue-culture-in-withania-somnifera-l-dunal-in-vitro-propagation-and-secondary-metabolite-production
#16
Shasmita, Manoj K Rai, Soumendra K Naik
Withania somnifera (L.) Dunal (family: Solanaceae), commonly known as "Indian Ginseng", is a medicinally and industrially important plant of the Indian subcontinent and other warmer parts of the world. The plant has multi-use medicinal potential and has been listed among 36 important cultivated medicinal plants of India that are in high demand for trade due to its pharmaceutical uses. The medicinal importance of this plant is mainly due to the presence of different types of steroidal lactones- withanolides in the roots and leaves...
December 26, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29196986/over-expression-of-atpap1-transcriptional-factor-enhances-phenolic-acid-production-in-transgenic-roots-of-leonurus-sibiricus-l-and-their-biological-activities
#17
Przemysław Sitarek, Tomasz Kowalczyk, Patricia Rijo, Adam J Białas, Marzena Wielanek, Halina Wysokińska, Catarina Garcia, Monika Toma, Tomasz Śliwiński, Ewa Skała
This study examines the production of five phenolic acids (chlorogenic acid, neochlorogenic acid, ferulic acid, caffeic acid and p-coumaric acid) following over-expression of AtPAP1 transcription factor by four transgenic root clones of Leonurus sibiricus after Agrobacterium rhizogenes transformation. The AtPAP1 expression level was estimated by quantitative real-time PCR. High levels of phenolic acids were found in the transgenic roots of L. sibiricus and were determined by high-performance liquid chromatography-mass spectrometry analysis...
January 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29156979/determination-of-lignans-phenolic-acids-and-antioxidant-capacity-in-transformed-hairy-root-culture-of-linum-usitatissimum
#18
Ahmed M M Gabr, Hoda B Mabrok, Emam A Abdel-Rahim, Mohamed K El-Bahr, Iryna Smetanska
Hairy root culture is a promising alternative method for the production of secondary metabolites. In this study, transformed root of Linum usitatissimum was established using Agrobacterium rhizogenes A4 strain from root cultures for lignans, phenolic acids and antioxidant capacity determination. Total lignin content (secoisolariciresinol diglucoside, secoisolariciresinol and matairesinol) was 55.5% higher in transformed root cultures than in the non-transformed root culture. Secoisolariciresinol was detected in higher concentration (2...
November 20, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29063187/composite-poplars-a-novel-tool-for-ectomycorrhizal-research
#19
Dimitri Neb, Arpita Das, Annette Hintelmann, Uwe Nehls
Composite poplars were used for ectomycorrhiza formation. Structurally normal mycorrhizas of transgenic roots revealed better fungal sugar support. Targeting fluorescent proteins to peroxisomes allowed easy in planta visualization of successful transformation. A bottle neck in ectomycorrhizal research is the time demand for generation of transgenic plants. An alternative strategy for such root-centered research might be the formation of the so-called composite plants, where transgenic roots are formed by non-transgenic shoots...
December 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/29053757/generation-of-composite-persea-americana-mill-avocado-plants-a-proof-of-concept-study
#20
S Ashok Prabhu, Buyani Ndlovu, Juanita Engelbrecht, Noëlani van den Berg
Avocado (Persea americana (Mill.)), an important commercial fruit, is severely affected by Phytophthora Root Rot in areas where the pathogen is prevalent. However, advances in molecular research are hindered by the lack of a high-throughput transient transformation system in this non-model plant. In this study, a proof-of-concept is demonstrated by the successful application of Agrobacterium rhizogenes-mediated plant transformation to produce composite avocado plants. Two ex vitro strategies were assessed on two avocado genotypes (Itzamna and A0...
2017: PloS One
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