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agrobacterium rhizogenes

Suvi T Häkkinen, Elisabeth Moyano, Rosa M Cusidó, Kirsi-Marja Oksman-Caldentey
Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds - commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells...
2016: Frontiers in Plant Science
Janhvi Mishra Rawat, Balwant Rawat, Susmita Mishra, Aakriti Bhandari, Rajneesh K Agnihotri, Anup Chandra
No abstract text is available yet for this article.
July 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Nete Kodahl, Renate Müller, Henrik Lütken
Plant transformation with the wild type Ri plasmid T-DNA of Agrobacterium rhizogenes is a promising method for breeding of compact plants and has been the subject of numerous studies. However, knowledge concerning the isolated functions of single genes and ORFs from the plasmid is limited. The rolB and ORF13 oncogenes of A. rhizogenes show considerable promise in plant breeding, but have not been comprehensively studied. Detailed information regarding the morphological impact of specific genes of the Ri plasmid will allow for optimized targeted breeding of plants transformed with the wild type Ri plasmid T-DNA...
November 2016: Plant Science: An International Journal of Experimental Plant Biology
Shahin Mardani-Nejad, Ramazan Ali Khavari-Nejad, Sara Saadatmand, Farzaneh Najafi, Parviz Aberoomand Azar
Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of rolB-transformed hairy roots and leaf callus of Catharanthus roseus. Hairy roots of the Catharanthus roseus were induced using Agrobacterium rhizogenes strain ATCC 15834 to transfer the rolB gene...
2016: Iranian Journal of Pharmaceutical Research: IJPR
Mrinalini Srivastava, Swati Sharma, Pratibha Misra
BACKGROUND: Rauwolfia serpentina and Solanum khasianum are well-known medicinally important plants contained important alkaloids in their different parts. Elicitation of these alkaloids is important because of associated pharmaceutical properties. Targeted metabolites were ajmaline and ajmalicine in R. serpentina; solasodine and α-solanine in S. khasianum. OBJECTIVE: Enhancement of secondary metabolites through biotic and abiotic elicitors in hairy root cultures of R...
May 2016: Pharmacognosy Magazine
Chang Ha Park, Young Seon Kim, Xiaohua Li, Haeng Hoon Kim, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Sook Young Lee, Sang Un Park
Carbohydrate sources play important roles in energy and growth of plants. Therefore, in this study, we investigated the optimal carbohydrate source in hairy root cultures (HRCs) of Scutellaria baicalensis infected with Agrobacterium rhizogenes strain R1000. The hairy roots were cultured in half-strength B5 liquid medium supplemented with seven different carbohydrates sources (sucrose, fructose, glucose, galactose, sorbitol, mannitol and maltose), each at a concentration of 100 mM, in order to identify the best carbon sources for the production of major flavones, such as wogonin, baicalin and baicalein...
June 2016: Natural Product Communications
Priscilla P Bettini, Massimiliano Marvasi, Fabiola Fani, Luigi Lazzara, Elena Cosi, Lorenzo Melani, Maria Luisa Mauro
Insertion of Agrobacterium rhizogenes rolB gene into plant genome affects plant development, hormone balance and defence. However, beside the current research, the overall transcriptional response and gene expression of rolB as a modulator in plant is unknown. Transformed rolB tomato plant (Solanum lycopersicum L.) cultivar Tondino has been used to investigate the differential expression profile. Tomato is a well-known model organism both at the genetic and molecular level, and one of the most important commercial food crops in the world...
October 1, 2016: Journal of Plant Physiology
Shivani Srivastava, Xavier A Conlan, David M Cahill, Alok Adholeya
Arbuscular mycorrhiza is a symbiotic association formed between plant roots and soil borne fungi that alter and at times improve the production of secondary metabolites. Detailed information is available on mycorrhizal development and its influence on plants grown under various edapho-climatic conditions, however, very little is known about their influence on transformed roots that are rich reserves of secondary metabolites. This raises the question of how mycorrhizal colonization progresses in transformed roots grown in vitro and whether the mycorrhizal fungus presence influences the production of secondary metabolites...
November 2016: Mycorrhiza
Jose A Valdes Franco, Ray Collier, Yi Wang, Naxin Huo, Yong Gu, Roger Thilmony, James G Thomson
This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild-type strain causes hairy root disease in dicots and has been used to make transgenic hairy root cultures and composite plants (nontransgenic shoots with transgenic roots). Disarmed variants of the strain have been used to produce stable transgenic monocot and dicot plants...
2016: Genome Announcements
Weina Hou, Preeti Shakya, Gregory Franklin
Hypericum perforatum (St John's wort) is a reservoir of diverse classes of biologically active and high value secondary metabolites, which captured the interest of both researchers and the pharmaceutical industry alike. Several studies and clinical trials have shown that H. perforatum extracts possess an astounding array of pharmacological properties. These properties include antidepressant, anti-inflammatory, antiviral, anti-cancer, and antibacterial activities; and are largely attributed to the naphtodianthrones and xanthones found in the genus...
2016: Frontiers in Plant Science
Peyman Habibi, Maria Fatima Grossi de Sa, André Luís Lopes da Silva, Abdullah Makhzoum, Jefferson da Luz Costa, Ivo Albertto Borghetti, Carlos Ricardo Soccol
Origanum vulgare L is commonly known as a wild marjoram and winter sweet which has been used in the traditional medicine due to its therapeutic effects as stimulant, anticancer, antioxidant, antibacterial, anti-inflammatory and many other diseases. A reliable gene transfer system via Agrobacterium rhizogenes and plant regeneration via hairy roots was established in O. vulgare for the first time. The frequency of induced hairy roots was different by modification of the co-cultivation medium elements after infection by Agrobacterium rhizogenes strains K599 and ATCC15834...
April 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Mohammad M Rana, Zhuo-Xiao Han, Da-Peng Song, Guo-Feng Liu, Da-Xiang Li, Xiao-Chun Wan, Alagarsamy Karthikeyan, Shu Wei
Tea (Camellia sinensis L.) is recalcitrant to Agrobacterium-mediated genetic transformation largely due to the bactericidal effects of tea polyphenols and phenolics oxidation induced by necrosis of explant tissue over the process of transformation. In this study, different antioxidants/adsorbents were added as supplements to the co-cultivation and post co-cultivation media to overcome these problems for the transformation improvement. Tea-cotyledon-derived calli were used as explants and Agrobacterium rhizognes strain ATCC 15834 was used as a mediator...
2016: International Journal of Molecular Sciences
Jonas Goossens, Nathan De Geyter, Alan Walton, Dominique Eeckhout, Jan Mertens, Jacob Pollier, Jennifer Fiallos, Annick De Keyser, Rebecca De Clercq, Jelle Van Leene, Kris Gevaert, Geert De Jaeger, Sofie Goormachtig, Alain Goossens
Tandem affinity purification coupled to mass spectrometry (TAP-MS) is one of the most powerful techniques to isolate protein complexes and elucidate protein interaction networks. Here, we describe the development of a TAP-MS strategy for the model legume Medicago truncatula, which is widely studied for its ability to produce valuable natural products and to engage in endosymbiotic interactions. As biological material, transgenic hairy roots, generated through Agrobacterium rhizogenes-mediated transformation of M...
July 5, 2016: Plant Journal: for Cell and Molecular Biology
Muthiah Joe Virgin Largia, Lakkakula Satish, Rajaiah Johnsi, Jayabalan Shilpha, Manikandan Ramesh
Agrobacterium rhizogenes mediated transformation has been experimented in leaf explants of the memory herb Bacopa monnieri in order to assess the regeneration potential of hairy roots (HR) followed by the elicitation of transformed plants for increased Bacoside A production. Out of the four strains tested, A4 and MTCC 532 derived HR exhibited regrowth in MS basal medium while MTCC 2364 derived HR showed regeneration in MS medium supplemented with suitable phyto hormones. R1000 derived HR possessed no regeneration potential...
August 2016: World Journal of Microbiology & Biotechnology
Chiara Lonoce, Reda Salem, Carla Marusic, Philippe V Jutras, Andrea Scaloni, Anna Maria Salzano, Sergio Lucretti, Herta Steinkellner, Eugenio Benvenuto, Marcello Donini
Hairy root (HR) cultures derived from Agrobacterium rhizogenes transformation of plant tissues are an advantageous biotechnological manufacturing platform due to the accumulation of recombinant proteins in an otherwise largely protein free culture medium. In this context, HRs descending from transgenic Nicotiana tabacum plants were successfully used for the production of several functional mAbs with plant-type glycans. Here, we expressed the tumor-targeting monoclonal antibody mAb H10 in HRs obtained either by infecting a transgenic N...
September 2016: Biotechnology Journal
Andrey Marchev, Zhenya Yordanova, Kalina Alipieva, Georgi Zahmanov, Snezhana Rusinova-Videva, Veneta Kapchina-Toteva, Svetlana Simova, Milena Popova, Milen I Georgiev
OBJECTIVES: To develop a protocol to transform Verbascum eriophorum and to study the metabolic differences between mother plants and hairy root culture by applying NMR and processing the datasets with chemometric tools. RESULTS: Verbascum eriophorum is a rare species with restricted distribution, which is poorly studied. Agrobacterium rhizogenes-mediated genetic transformation of V. eriophorum and hairy root culture induction are reported for the first time. To determine metabolic alterations, V...
September 2016: Biotechnology Letters
Ray Collier, Jennifer Bragg, Bryan T Hernandez, John P Vogel, Roger Thilmony
The genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brachypodium has relied mainly on Agrobacterium tumefaciens strain AGL1. Currently the antibiotic hygromycin B has been the selective agent of choice for robust identification of transgenic calli in Brachypodium distachyon and Brachypodium sylvaticum but few other chemicals have been shown to work as well for selection of transgenic Brachypodium cells in tissue culture...
2016: Frontiers in Plant Science
L Bosmans, B Van Calenberge, A Paeleman, R Moerkens, L Wittemans, S Van Kerckhove, R De Mot, B Lievens, H Rediers
AIMS: Rhizogenic Agrobacterium strains are the causative agent of hairy root disease (HRD), an increasing problem in the hydroponic cultivation of tomato and cucumber in Europe. A previous study has demonstrated that different lineages of rhizogenic agrobacteria are able to form biofilms. Although hydrogen peroxide (H2 O2 ) is a frequently used biocide in hydroponic systems, until now its effectiveness to remove rhizogenic agrobacteria has not been unequivocally demonstrated. Therefore, the main objective of this study was to assess the efficacy of H2 O2 in controlling Agrobacterium-containing biofilms...
August 2016: Journal of Applied Microbiology
Galina Veremeichik, Victor Bulgakov, Yury Shkryl
Expression of rol genes from Agrobacterium rhizogenes induces reprogramming of transformed plant cells and provokes pleiotropic effects on primary and secondary metabolism. We have previously established that the rolB and rolC genes impair reactive oxygen species (ROS) generation in transformed cells of Rubia cordifolia and Arabidopsis thaliana. In the present investigation, we tested whether this effect is associated with changes in the expression levels of NADPH oxidases, which are considered to be the primary source of ROS during plant-microbe interactions...
August 2016: Plant Physiology and Biochemistry: PPB
Bushra Hafeez Kiani, John Suberu, Bushra Mirza
BACKGROUND: Malaria is causing more than half of a million deaths and 214 million clinical cases annually. Despite tremendous efforts for the control of malaria, the global morbidity and mortality have not been significantly changed in the last 50 years. Artemisinin, extracted from the medicinal plant Artemisia sp. is an effective anti-malarial drug. In 2015, elucidation of the effectiveness of artemisinin as a potent anti-malarial drug was acknowledged with a Nobel prize. Owing to the tight market and low yield of artemisinin, an economical way to increase its production is to increase its content in Artemisia sp...
2016: Malaria Journal
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