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agrobacterium rhizogenes

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https://www.readbyqxmd.com/read/28401459/optimizing-culture-conditions-for-establishment-of-hairy-root-culture-of-semecarpus-anacardium-l
#1
Bhuban Mohan Panda, Urmil J Mehta, Sulekha Hazra
Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25-30 days of infection. Optimum transformation frequency of 61% was achieved on leaf explants with ATCC15834 strain. Infection time of 30 min resulted in greater transformation frequency compared to 10 and 20 min, respectively...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28400855/ex-vitro-hairy-root-induction-in-detached-peanut-leaves-for-plant-nematode-interaction-studies
#2
Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro
BACKGROUND: Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome studies of wild Arachis species, which harbor resistance to a number of pests and diseases, disclosed several candidate genes for M. arenaria resistance. Peanut is recalcitrant to genetic transformation, so the use of Agrobacterium rhizogenes-derived hairy roots emerged as an alternative for in-root functional characterization of these candidate genes...
2017: Plant Methods
https://www.readbyqxmd.com/read/28393691/biotechnological-approaches-for-production-of-anti-cancerous-compounds-resveratrol-podophyllotoxin-and-zerumbone
#3
Krishnadas Nandagopal, Mihir Halder, Biswabhusan Dash, Sanghamitra Nayak, Sumita Jha
Secondary metabolites from numerous plant sources have been developed as anti-cancer reagents and compounds such as resveratrol, podophyllotoxin and zerumbone are of particular importance in this regard. Since their de novo chemical synthesis is both arduous and commercially expensive, there has been an impetus to develop viable, biotechnological methods of production. Accordingly, this review focuses on recent developments in the field, highlighting the use of micropropagation, cell suspension cultures, callus cultures, hairy root cultures, recombinant microbes and genetically modified higher plants...
April 4, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28382027/potential-for-biocontrol-of-hairy-root-disease-by-a-paenibacillus-clade
#4
Lien Bosmans, Irene De Bruijn, Saskia Gerards, Rob Moerkens, Lore Van Looveren, Lieve Wittemans, Bart Van Calenberge, Anneleen Paeleman, Stefan Van Kerckhove, René De Mot, Jef Rozenski, Hans Rediers, Jos M Raaijmakers, Bart Lievens
Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28358854/validation-of-a-hairy-roots-system-to-study-soybean-soybean-aphid-interactions
#5
Stephanie C Morriss, Matthew E Studham, Gregory L Tylka, Gustavo C MacIntosh
The soybean aphid (Aphis glycines) is one of the main insect pests of soybean (Glycine max) worldwide. Genomics approaches have provided important data on transcriptome changes, both in the insect and in the plant, in response to the plant-aphid interaction. However, the difficulties to transform soybean and to rear soybean aphid on artificial media have hindered our ability to systematically test the function of genes identified by those analyses as mediators of plant resistance to the insect. An efficient approach to produce transgenic soybean material is the production of transformed hairy roots using Agrobacterium rhizogenes; however, soybean aphids colonize leaves or stems and thus this approach has not been utilized...
2017: PloS One
https://www.readbyqxmd.com/read/28340896/bioactive-constituents-from-transformed-root-cultures-of-nepeta-teydea
#6
Braulio M Fraga, Azucena González-Coloma, Sergio Alegre-Gómez, Matías López-Rodríguez, Leonardo J Amador, Carmen E Díaz
A phytochemical study of an extract from transformed root cultures of Nepeta teydea, induced by Agrobacterium rhizogenes, led to the isolation of the following new compounds: the sesquiterpene (-)-cinalbicol, the diterpene teydeadione (6,11,14-trihydroxy-12-methoxy-abieta-5,8,11,13,15-penten-7-one), a degraded C23-triterpene (teydealdehyde) and three fatty acid esters of lanosta-7,24-dien-3β-ol. The propyl ester of rosmarinic acid was also isolated for the first time from a natural source. In addition, two dehydroabietane diterpenes, eight triterpenes and eighteen known phenolic compounds were obtained...
January 2017: Phytochemistry
https://www.readbyqxmd.com/read/28330254/enhanced-production-of-an-anti-malarial-compound-artesunate-by-hairy-root-cultures-and-phytochemical-analysis-of-artemisia-pallens-wall
#7
Zarna Pala, Vishnu Shukla, Anshu Alok, Subhash Kudale, Neetin Desai
Artemisinin and its derivatives are still one of the most effective drugs for the treatment of malaria. Artemisia pallens commonly known as Dhavanam, is an aromatic herb belonging to the family Asteraceae. Increasing the artemisinin content of A. pallens by genetic engineering would improve the availability of this much needed drug. In the present study, Agrobacterium rhizogenes (strain NCIM 5140) mediated genetic transformation of Artemisia pallens were carried out for hairy root induction. The effect of different media (Half MS, MS, MS along with BAP 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28271384/hairy-root-culture-optimization-and-resveratrol-production-from-vitis-vinifera-subsp-sylvesteris
#8
Sayed Mehdi Hosseini, Bahman Bahramnejad, Hamed Douleti Baneh, Aryo Emamifar, Paul H Goodwin
Resveratrol is a polyphenolic compound produced in very low levels in grapes. To achieve high yield of resveratrol in wild grape, three Agrobacterium rhizogenes strains, Ar318, ArA4 and LBA9402, were used to induce hairy roots following infection of internodes, nodes or petioles of in vitro grown Vitis vinifera subsp. sylvesteris accessions W2 and W16, and cultivar Rasha. The effects of inoculation time, age of explants, bacterial concentration and co-cultivation times were examined on the efficiency of the production of hairy roots...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28256553/targeted-mutagenesis-in-the-medicinal-plant-salvia-miltiorrhiza
#9
Bin Li, Guanghong Cui, Guoan Shen, Zhilai Zhan, Luqi Huang, Jiachun Chen, Xiaoquan Qi
CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/Cas9 to precisely knock out the committed diterpene synthase gene (SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a traditional Chinese medicinal herb with significant pharmacological activities, such as vasorelaxation, protection against ischemia-reperfusion injury, and antiarrhythmic effects. Three homozygous and eight chimeric mutants were obtained from 26 independent transgenic hairy root lines by Agrobacterium rhizogenes-mediated transformation...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28203245/small-rnas-derived-from-the-t-dna-of-agrobacterium-rhizogenes-in-hairy-roots-of-phaseolus-vulgaris
#10
Pablo Peláez, Alejandrina Hernández-López, Georgina Estrada-Navarrete, Federico Sanchez
Agrobacterium rhizogenes is a pathogenic bacteria that causes hairy root disease by transferring bacterial DNA into the plant genome. It is an essential tool for industry and research due to its capacity to produce genetically modified roots and whole organisms. Here, we identified and characterized small RNAs generated from the transfer DNA (T-DNA) of A. rhizogenes in hairy roots of common bean (Phaseolus vulgaris). Distinct abundant A. rhizogenes T-DNA-derived small RNAs (ArT-sRNAs) belonging to several oncogenes were detected in hairy roots using high-throughput sequencing...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28137207/patent-survey-of-resveratrol-taxol-podophyllotoxin-withanolides-and-their-derivatives-used-in-anticancer-therapy
#11
Krishnadas Nandagopal, Shreya Routh
BACKGROUND: Resveratrol, taxol, podophyllotoxin, withanolides and their derivatives find applications in anti-cancer therapy. They are plant-derived compounds whose chemical structures and synthesis limit their natural availability and restrict a large-scale industrial production. Hence, their production by various biotechnological approaches may hold promise for a continuous and reliable mode of supply. OBJECTIVE: We review process and product patents in this regard...
January 27, 2017: Recent Patents on Biotechnology
https://www.readbyqxmd.com/read/27943249/metabolic-engineering-of-fatty-alcohol-production-in-transgenic-hairy-roots-of-crambe-abyssinica
#12
Magdalena Miklaszewska, Antoni Banaś, Aleksandra Królicka
Biotechnological production of fatty alcohols, important raw materials in the chemical industry, has been receiving considerable attention in recent years. Fatty alcohols are formed by the reduction of fatty acyl-CoAs or fatty acyl-ACPs catalyzed by a fatty acyl reductase (FAR). In this study, we introduced genes encoding FARs from Arabidopsis thaliana (AtFAR5) and Simmondsia chinensis (ScFAR) into Crambe abyssinica hairy roots via Agrobacterium rhizogenes-mediated transformation. The efficiency of the transformation ranged between 30 and 45%...
June 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27880021/engineering-the-expression-level-of-cytosolic-nucleoside-diphosphate-kinase-in-transgenic-solanum-tuberosum-roots-alters-growth-respiration-and-carbon-metabolism
#13
Sonia Dorion, Audrey Clendenning, Jean Rivoal
Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme that catalyzes the transfer of the γ-phosphate from a donor nucleoside triphosphate to an acceptor nucleoside diphosphate. In this study we used a targeted metabolomic approach and measurement of physiological parameters to report the effects of the genetic manipulation of cytosolic NDPK (NDPK1) expression on physiology and carbon metabolism in potato (Solanum tuberosum) roots. Sense and antisense NDPK1 constructs were introduced in potato using Agrobacterium rhizogenes to generate a population of root clones displaying a 40-fold difference in NDPK activity...
March 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27813337/still-stable-after-11-years-a-catharanthus-roseus-hairy-root-line-maintains-inducible-expression-of-anthranilate-synthase
#14
Jiayi Sun, Li Ma, Ka-Yiu San, Christie A M Peebles
Hairy root cultures generated using Agrobacterium rhizogenes are an extensively investigated system for the overproduction of various secondary metabolite based pharmaceuticals and chemicals. This study demonstrated a transgenic Catharanthus roseus hairy root line carrying a feedback-insensitive anthranilate synthase (AS) maintained chemical and genetic stability for 11 years. The AS gene was originally inserted in the hairy root genome under the control of a glucocorticoid inducible promoter. After 11 years continuous maintenance of this hairy root line, genomic PCR of the ASA gene showed the presence of ASA gene in the genome...
November 4, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27746806/exploring-the-metabolic-stability-of-engineered-hairy-roots-after-16-years-maintenance
#15
Suvi T Häkkinen, Elisabeth Moyano, Rosa M Cusidó, Kirsi-Marja Oksman-Caldentey
Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds - commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27729729/retracted%C3%A2-article-influence-of-agrobacterium-rhizogenes-strains-acitosyringone-inoculum-size-and-temperature-on-production-of-active-ingredients-from-picrorhiza-kurrooa
#16
Janhvi Mishra Rawat, Balwant Rawat, Susmita Mishra, Aakriti Bhandari, Rajneesh K Agnihotri, Anup Chandra
No abstract text is available yet for this article.
July 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27717457/the-agrobacterium-rhizogenes-oncogenes-rolb-and-orf13-increase-formation-of-generative-shoots-and-induce-dwarfism-in-arabidopsis-thaliana-l-heynh
#17
Nete Kodahl, Renate Müller, Henrik Lütken
Plant transformation with the wild type Ri plasmid T-DNA of Agrobacterium rhizogenes is a promising method for breeding of compact plants and has been the subject of numerous studies. However, knowledge concerning the isolated functions of single genes and ORFs from the plasmid is limited. The rolB and ORF13 oncogenes of A. rhizogenes show considerable promise in plant breeding, but have not been comprehensively studied. Detailed information regarding the morphological impact of specific genes of the Ri plasmid will allow for optimized targeted breeding of plants transformed with the wild type Ri plasmid T-DNA...
November 2016: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/27642325/potent-antioxidant-properties-of-rolb-transformed-catharanthus-roseus-l-g-don
#18
Shahin Mardani-Nejad, Ramazan Ali Khavari-Nejad, Sara Saadatmand, Farzaneh Najafi, Parviz Aberoomand Azar
Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of rolB-transformed hairy roots and leaf callus of Catharanthus roseus. Hairy roots of the Catharanthus roseus were induced using Agrobacterium rhizogenes strain ATCC 15834 to transfer the rolB gene...
2016: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/27563218/elicitation-based-enhancement-of-secondary-metabolites-in-rauwolfia-serpentina-and-solanum-khasianum-hairy-root-cultures
#19
Mrinalini Srivastava, Swati Sharma, Pratibha Misra
BACKGROUND: Rauwolfia serpentina and Solanum khasianum are well-known medicinally important plants contained important alkaloids in their different parts. Elicitation of these alkaloids is important because of associated pharmaceutical properties. Targeted metabolites were ajmaline and ajmalicine in R. serpentina; solasodine and α-solanine in S. khasianum. OBJECTIVE: Enhancement of secondary metabolites through biotic and abiotic elicitors in hairy root cultures of R...
May 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27534120/influence-of-different-carbohydrates-on-flavonoid-accumulation-in-hairy-root-cultures-of-scutellaria-baicalensis
#20
COMPARATIVE STUDY
Chang Ha Park, Young Seon Kim, Xiaohua Li, Haeng Hoon Kim, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Sook Young Lee, Sang Un Park
Carbohydrate sources play important roles in energy and growth of plants. Therefore, in this study, we investigated the optimal carbohydrate source in hairy root cultures (HRCs) of Scutellaria baicalensis infected with Agrobacterium rhizogenes strain R1000. The hairy roots were cultured in half-strength B5 liquid medium supplemented with seven different carbohydrates sources (sucrose, fructose, glucose, galactose, sorbitol, mannitol and maltose), each at a concentration of 100 mM, in order to identify the best carbon sources for the production of major flavones, such as wogonin, baicalin and baicalein...
June 2016: Natural Product Communications
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