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agrobacterium rhizogenes

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https://www.readbyqxmd.com/read/29063187/composite-poplars-a-novel-tool-for-ectomycorrhizal-research
#1
Dimitri Neb, Arpita Das, Annette Hintelmann, Uwe Nehls
Composite poplars were used for ectomycorrhiza formation. Structurally normal mycorrhizas of transgenic roots revealed better fungal sugar support. Targeting fluorescent proteins to peroxisomes allowed easy in planta visualization of successful transformation. A bottle neck in ectomycorrhizal research is the time demand for generation of transgenic plants. An alternative strategy for such root-centered research might be the formation of the so-called composite plants, where transgenic roots are formed by non-transgenic shoots...
December 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/29053757/generation-of-composite-persea-americana-mill-avocado-plants-a-proof-of-concept-study
#2
S Ashok Prabhu, Buyani Ndlovu, Juanita Engelbrecht, Noëlani van den Berg
Avocado (Persea americana (Mill.)), an important commercial fruit, is severely affected by Phytophthora Root Rot in areas where the pathogen is prevalent. However, advances in molecular research are hindered by the lack of a high-throughput transient transformation system in this non-model plant. In this study, a proof-of-concept is demonstrated by the successful application of Agrobacterium rhizogenes-mediated plant transformation to produce composite avocado plants. Two ex vitro strategies were assessed on two avocado genotypes (Itzamna and A0...
2017: PloS One
https://www.readbyqxmd.com/read/29022401/green-synthesis-of-silver-nanoparticles-using-transgenic-nicotiana-tabacum-callus-culture-expressing-silicatein-gene-from-marine-sponge-latrunculia-oparinae
#3
Yuri N Shkryl, Galina N Veremeichik, Dmitriy G Kamenev, Tatiana Y Gorpenchenko, Yulia A Yugay, Dmitriy V Mashtalyar, Aleksander V Nepomnyaschiy, Tatiana V Avramenko, Aleksandr A Karabtsov, Vladimir V Ivanov, Victor P Bulgakov, Sergey V Gnedenkov, Yury N Kulchin, Yury N Zhuravlev
In the present investigation, transgenic tobacco callus cultures and plants overexpressing the silicatein gene LoSilA1 from marine sponge Latrunculia oparinae were obtained and their bioreduction behaviour for the synthesis of silver nanoparticles (AgNPs) was studied. Synthesized nanoparticles were characterized using UV-visible spectroscopy, Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic flame electron microscopy (AFM) and nanoparticle tracking analysis (NTA)...
October 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28966626/natural-agrobacterium-transformants-recent-results-and-some-theoretical-considerations
#4
REVIEW
Ke Chen, Léon Otten
Agrobacterium rhizogenes causes hairy root growth on a large number of plant species. It does so by transferring specific DNA fragments (T-DNA) from its root-inducing plasmid (pRi) into plant cells. Expression of T-DNA genes leads to abnormal root growth and production of specific metabolites (opines) which are taken up by the bacterium and used for its growth. Recent work has shown that several Nicotiana, Linaria, and Ipomoea species contain T-DNA genes from A. rhizogenes in their genomes. Plants carrying such T-DNAs (called cellular T-DNA or cT-DNA) can be considered as natural transformants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28956383/-factors-influencing-induction-and-in-vitro-culture-of-hairy-roots-in-phytolacca-americana-l
#5
Heping Shi, Yuanfeng Zhu, Po Keung Eric Tsang, Cheuk Fai Stephen Chow, Zhen'ao Yu, Shengqin Huang
To use hairy roots for producing medicinal ingredients of Phytolacca americana L. we studied the factors influencing the induction and in vitro culture. Hairy roots could be incited from the veins of cut surface (morphological lower) of P. americana L. leaf explants around 18 days after infection with the strain of Agrobacterium rhizogenes ATCC15834. The highest rooting rate, 70%, was obtained when leaf explants were pre-cultured for 1 day, infected for 20 min, and co-cultured for 4 days. The transformation was confirmed by PCR amplification of rolC of Ri plasmid and silica gel thin-layer chromatography of opines from P...
February 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28835782/genetic-transformation-and-hairy-root-induction-enhance-the-antioxidant-potential-of-lactuca-serriola-l
#6
Mohamed A El-Esawi, Amr Elkelish, Hosam O Elansary, Hayssam M Ali, Mohamed Elshikh, Jacques Witczak, Margaret Ahmad
Lactuca serriola L. is a herbaceous species, used for human nutrition and medicinal purposes. The high antioxidant capacity of L. serriola indicates the possibility of enhancing its edible and health potential by increasing the flavonoid and phenolic contents. The present study aimed at enhancing the production of phenolics and flavonoids by hairy root cultures in Lactuca serriola transformed with Agrobacterium rhizogenes strain AR15834 harbouring the rolB gene. The genetic transformation of rolB in transformed roots was validated, and rolB expression level was evaluated using real-time qPCR analysis...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28800637/biologically-active-recombinant-human-erythropoietin-expressed-in-hairy-root-cultures-and-regenerated-plantlets-of-nicotiana-tabacum-l
#7
Poornima Devi Gurusamy, Holger Schäfer, Siva Ramamoorthy, Michael Wink
Hairy root culture is a potential alternative to conventional mammalian cell culture to produce recombinant proteins due to its ease in protein recovery, low costs and absence of potentially human pathogenic contaminants. The current study focussed to develop a new platform of a hairy root culture system from Nicotiana tabacum for the production of recombinant human EPO (rhEPO), which is regularly produced in mammalian cells. The human EPO construct was amplified with C-terminal hexahistidine tag from a cDNA of Caco-2 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28769947/heterologous-expression-of-panax-ginseng-pgtip1-confers-enhanced-salt-tolerance-of-soybean-cotyledon-hairy-roots-composite-and-whole-plants
#8
Jing An, Zhenmin Hu, Benning Che, Haiying Chen, Bingjun Yu, Weiming Cai
The Panax ginseng TIP gene PgTIP1 was previously demonstrated to have high water channel activity by its heterologous expression in Xenopus laevis oocytes and in yeast; it also plays a significant role in growth of PgTIP1-transgenic Arabidopsis plants under favorable conditions and has enhanced tolerance toward salt and drought treatment. In this work, we first investigated the physiological effects of heterologous PgTIP1 expression in soybean cotyledon hairy roots or composite plants mediated by Agrobacterium rhizogenes toward enhanced salt tolerance...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28709614/generation-of-recombinant-protein-shells-of-johnson-grass-chlorotic-stripe-mosaic-virus-in-tobacco-plants-and-their-use-as-drug-carrier
#9
E Alemzadeh, K Izadpanah, F Ahmadi
The development and use of virus-like particles (VLPs) is a growing field with a powerful potential in generation of nanoparticles. In the present study we have attempted to generate and use empty shells of Johnson grass chlorotic stripe mosaic virus (JgCSMV, a member of the genus Aureusvirus, family Tombusviridae) as VLP nanoparticles for drug loading. In order to successfully produce recombinant JgCSMV-derived VLPs, we followed an approach based on cloning of the JgCSMV CP gene into pBI121 vector and introduction of the latter into Agrobacterium rhizogenes and transformation of tobacco cells for coat protein expression...
October 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28687763/a-tyrosine-aminotransferase-involved-in-rosmarinic-acid-biosynthesis-in-prunella-vulgaris-l
#10
Mei Ru, Kunru Wang, Zhenqing Bai, Liang Peng, Shaoxuan He, Yong Wang, Zongsuo Liang
Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28567046/transcriptome-analysis-of-salicylic-acid-treatment-in-rehmannia-glutinosa-hairy-roots-using-rna-seq-technique-for-identification-of-genes-involved-in-acteoside-biosynthesis
#11
Fengqing Wang, Jingyu Zhi, Zhongyi Zhang, Lina Wang, Yanfei Suo, Caixia Xie, Mingjie Li, Bao Zhang, Jiafang Du, Li Gu, Hongzheng Sun
Rehmannia glutinosa is a common bulk medicinal material that has been widely used in China due to its active ingredients. Acteoside, one of the ingredients, has antioxidant, antinephritic, anti-inflammatory, hepatoprotective, immunomodulatory, and neuroprotective effects, is usually selected as a quality-control component for R. glutinosa herb in the Chinese Pharmacopeia. The acteoside biosynthesis pathway in R. glutinosa has not yet been clearly established. Herein, we describe the establishment of a genetic transformation system for R...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28554466/hairy-root-transgene-expression-analysis-of-a-secretory-peroxidase-pvpox1-from-common-bean-infected-by-fusarium-wilt
#12
Renfeng Xue, Xingbo Wu, Yingjie Wang, Yan Zhuang, Jian Chen, Jing Wu, Weide Ge, Lanfen Wang, Shumin Wang, Matthew W Blair
Plant peroxidases (POXs) are one of the most important redox enzymes in the defense responses. However, the large number of different plant POX genes makes it necessary to carefully confirm the function of each paralogous POX gene in specific tissues and disease interactions. Fusarium wilt is a devastating disease of common bean caused by Fusarium oxysporum f. sp. phaseoli. In this study, we evaluated a peroxidase gene, PvPOX1, from a resistant common bean genotype, CAAS260205 and provided direct evidence for PvPOX1's role in resistance by transforming the resistant allele into a susceptible common bean genotype, BRB130, via hairy root transformation using Agrobacterium rhizogenes...
July 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28553065/enhanced-production-of-hyoscyamine-and-scopolamine-from-genetically-transformed-root-culture-of-hyoscyamus-reticulatus-l-elicited-by-iron-oxide-nanoparticles
#13
Fereshte Moharrami, Bahman Hosseini, Ali Sharafi, Manouchehr Farjaminezhad
The medicinal plant Hyoscyamus reticulatus L. is a rich source of hyoscyamine and scopolamine, the tropane alkaloids. The use of hairy root cultures has focused significant attention on production of important metabolites such as stable tropane alkaloid production. Elicitation is an effective approach to induce secondary metabolite biosynthetic pathways. Hairy roots were derived from cotyledon explants inoculated with Agrobacterium rhizogenes and elicited by iron oxide nanoparticles (FeNPs) at different concentrations (0, 450, 900, 1800, and 3600 mg L(-1)) for different exposure times (24, 48, and 72 h)...
2017: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
https://www.readbyqxmd.com/read/28401459/optimizing-culture-conditions-for-establishment-of-hairy-root-culture-of-semecarpus-anacardium-l
#14
Bhuban Mohan Panda, Urmil J Mehta, Sulekha Hazra
Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25-30 days of infection. Optimum transformation frequency of 61% was achieved on leaf explants with ATCC15834 strain. Infection time of 30 min resulted in greater transformation frequency compared to 10 and 20 min, respectively...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28400855/ex-vitro-hairy-root-induction-in-detached-peanut-leaves-for-plant-nematode-interaction-studies
#15
Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro
BACKGROUND: Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome studies of wild Arachis species, which harbor resistance to a number of pests and diseases, disclosed several candidate genes for M. arenaria resistance. Peanut is recalcitrant to genetic transformation, so the use of Agrobacterium rhizogenes-derived hairy roots emerged as an alternative for in-root functional characterization of these candidate genes...
2017: Plant Methods
https://www.readbyqxmd.com/read/28393691/biotechnological-approaches-for-production-of-anti-cancerous-compounds-resveratrol-podophyllotoxin-and-zerumbone
#16
Krishnadas Nandagopal, Mihir Halder, Biswabhusan Dash, Sanghamitra Nayak, Sumita Jha
Secondary metabolites from numerous plant sources have been developed as anti-cancer reagents and compounds such as resveratrol, podophyllotoxin and zerumbone are of particular importance in this regard. Since their de novo chemical synthesis is both arduous and commercially expensive, there has been an impetus to develop viable, biotechnological methods of production. Accordingly, this review focuses on recent developments in the field, highlighting the use of micropropagation, cell suspension cultures, callus cultures, hairy root cultures, recombinant microbes and genetically modified higher plants...
April 4, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28382027/potential-for-biocontrol-of-hairy-root-disease-by-a-paenibacillus-clade
#17
Lien Bosmans, Irene De Bruijn, Saskia Gerards, Rob Moerkens, Lore Van Looveren, Lieve Wittemans, Bart Van Calenberge, Anneleen Paeleman, Stefan Van Kerckhove, René De Mot, Jef Rozenski, Hans Rediers, Jos M Raaijmakers, Bart Lievens
Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28358854/validation-of-a-hairy-roots-system-to-study-soybean-soybean-aphid-interactions
#18
Stephanie C Morriss, Matthew E Studham, Gregory L Tylka, Gustavo C MacIntosh
The soybean aphid (Aphis glycines) is one of the main insect pests of soybean (Glycine max) worldwide. Genomics approaches have provided important data on transcriptome changes, both in the insect and in the plant, in response to the plant-aphid interaction. However, the difficulties to transform soybean and to rear soybean aphid on artificial media have hindered our ability to systematically test the function of genes identified by those analyses as mediators of plant resistance to the insect. An efficient approach to produce transgenic soybean material is the production of transformed hairy roots using Agrobacterium rhizogenes; however, soybean aphids colonize leaves or stems and thus this approach has not been utilized...
2017: PloS One
https://www.readbyqxmd.com/read/28340896/bioactive-constituents-from-transformed-root-cultures-of-nepeta-teydea
#19
Braulio M Fraga, Azucena González-Coloma, Sergio Alegre-Gómez, Matías López-Rodríguez, Leonardo J Amador, Carmen E Díaz
A phytochemical study of an extract from transformed root cultures of Nepeta teydea, induced by Agrobacterium rhizogenes, led to the isolation of the following new compounds: the sesquiterpene (-)-cinalbicol, the diterpene teydeadione (6,11,14-trihydroxy-12-methoxy-abieta-5,8,11,13,15-penten-7-one), a degraded C23-triterpene (teydealdehyde) and three fatty acid esters of lanosta-7,24-dien-3β-ol. The propyl ester of rosmarinic acid was also isolated for the first time from a natural source. In addition, two dehydroabietane diterpenes, eight triterpenes and eighteen known phenolic compounds were obtained...
January 2017: Phytochemistry
https://www.readbyqxmd.com/read/28330254/enhanced-production-of-an-anti-malarial-compound-artesunate-by-hairy-root-cultures-and-phytochemical-analysis-of-artemisia-pallens-wall
#20
Zarna Pala, Vishnu Shukla, Anshu Alok, Subhash Kudale, Neetin Desai
Artemisinin and its derivatives are still one of the most effective drugs for the treatment of malaria. Artemisia pallens commonly known as Dhavanam, is an aromatic herb belonging to the family Asteraceae. Increasing the artemisinin content of A. pallens by genetic engineering would improve the availability of this much needed drug. In the present study, Agrobacterium rhizogenes (strain NCIM 5140) mediated genetic transformation of Artemisia pallens were carried out for hairy root induction. The effect of different media (Half MS, MS, MS along with BAP 0...
December 2016: 3 Biotech
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