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https://www.readbyqxmd.com/read/29241295/in-vitro-selection-and-characterization-of-dna-aptamers-to-a-small-molecule-target
#1
Annamaria Ruscito, Erin M McConnell, Anna Koudrina, Ranganathan Velu, Christopher Mattice, Vernon Hunt, Maureen McKeague, Maria C DeRosa
Aptamers, synthetic oligonucleotide-based molecular recognition probes, have found use in a wide array of biosensing technologies based on their tight and highly selective binding to a variety of molecular targets. However, the inherent challenges associated with the selection and characterization of aptamers for small molecule targets have resulted in their underrepresentation, despite the need for small molecule detection in fields such as medicine, the environment, and agriculture. This protocol describes the steps in the selection, sequencing, affinity characterization, and truncation of DNA aptamers that are specific for small molecule targets...
December 14, 2017: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/29240559/fluorescence-detection-enumeration-and-characterization-of-single-circulating-cells-in-vivo-technology-applications-and-future-prospects
#2
Carolin Hartmann, Roshani Patil, Charles P Lin, Mark Niedre
There are many diseases and biological processes that involve circulating cells in the bloodstream, such as cancer metastasis, immunology, reproductive medicine, and stem cell therapies. This has driven significant interest in new technologies for the study of circulating cells in small animal research models and clinically. Most currently used methods require drawing and enriching blood samples from the body, but these suffer from a number of limitations. In contrast, 'in vivo flow cytometry' (IVFC) refers to set of technologies that allow study of cells directly in the bloodstream of the organism in vivo...
December 14, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29240347/genomic-data-into-everyday-work-of-a-medical-practitioner-digital-tools-for-decision-making
#3
Sakari Jokiranta, Kristina Hotakainen, Iiris Salonen, Pasi Pöllänen, Kai-Petri Hänninen, Jari Forsström, Ilkka Kunnamo
Recent technological development has enabled fast and cost-effective simultaneous analyses of several gene variants or sequence of even the whole genome. For medical practitioners this has created challenges although genomic information may be clinically useful in new applications such as finding out individual risk for diseases influenced by as many as 50,000 variable DNA regions or in detecting pharmacogenetic risks prior to prescribing a medicine. New digital tools have paved the way for utilization of genomic data via easy access and clear clinical interpretation for both doctor and patient...
2017: Duodecim; Lääketieteellinen Aikakauskirja
https://www.readbyqxmd.com/read/29239945/error-grid-analysis-for-arterial-pressure-method-comparison-studies
#4
Bernd Saugel, Oliver Grothe, Julia Y Nicklas
The measurement of arterial pressure (AP) is a key component of hemodynamic monitoring. A variety of different innovative AP monitoring technologies became recently available. The decision to use these technologies must be based on their measurement performance in validation studies. These studies are AP method comparison studies comparing a new method ("test method") with a reference method. In these studies, different comparative statistical tests are used including correlation analysis, Bland-Altman analysis, and trending analysis...
December 11, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29239200/digital-health-and-perioperative-care
#5
Theofanis Fotis
According to the U.S. Food and Drug Administration 'the broad scope of digital health includes categories such as mobile health (mHealth), health information technology (IT), wearable devices, telehealth and telemedicine, and personalised medicine, and is used by providers and other stakeholders in their efforts to reduce inefficiencies, improve access, reduce costs, increase quality, and make medicine more personalised for patients (FDA 2016). More recently, Paul Sonier, a digital health strategist and founder of the Linkedin digital health group with more than 40,000 members, defined digital health as 'the convergence of the digital and genomic revolutions with health, healthcare, living, and society' (storyofdigitalhealth...
June 2017: Journal of Perioperative Practice
https://www.readbyqxmd.com/read/29239153/time-to-change-the-classical-vision-of-coagulation-in-liver-disease-from-the-balance-disequilibrium-to-the-systems-biology-network-modelling
#6
Annabel Blasi, Joan C Reverter
The longstanding dogma that patients with liver disease have a hemostasis related bleeding tendency is neglected from the last 10 years however, despite this tremendous change of paradigm no longer progress had been made in this field. A mathematical model is a description of a system using mathematical concepts and language. Mathematical models are able to analyze large amount of data and they can make predictions about behavior. Mathematical models are used in the social sciences, engineering as well as in medicine...
December 13, 2017: Minerva Anestesiologica
https://www.readbyqxmd.com/read/29234404/modernization-trends-of-infertility-treatment-of-traditional-korean-medicine
#7
REVIEW
Jang-Kyung Park, Dong-Il Kim
Despite the development of assisted reproductive technology (ART), it is difficult to increase the implantation rate. In Korea, Traditional Korean Medicine, including herbal medicine, is an important component of infertility treatment. Korean medical doctors who are treating infertility often use herbal medicine to promote implantation. In this article, as one of the research works on modernization of Traditional Korean Medicine, we investigated the experimental studies to clarify the effects of herbal medicines that are traditionally used to promote pregnancy...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29234120/oral-epithelial-cell-sheets-engraftment-for-esophageal-strictures-after-endoscopic-submucosal-dissection-of-squamous-cell-carcinoma-and-airplane-transportation
#8
Naoyuki Yamaguchi, Hajime Isomoto, Shinichiro Kobayashi, Nobuo Kanai, Kengo Kanetaka, Yusuke Sakai, Yoshiyuki Kasai, Ryo Takagi, Takeshi Ohki, Hiroko Fukuda, Tsutomu Kanda, Kazuhiro Nagai, Izumi Asahina, Kazuhiko Nakao, Masayuki Yamato, Teruo Okano, Susumu Eguchi
Endoscopic submucosal dissection (ESD) permits en bloc removal of superficial oesophageal squamous cell carcinoma (ESCC). However, post-procedure stricture is common after ESD for widespread tumours, and multiple endoscopic balloon dilation (EBD) procedures are required. We aimed to evaluate the safety and effectiveness of endoscopic transplantation of tissue-engineered autologous oral mucosal epithelial cell sheets that had been transported by air over a distance of 1200 km in controlling postprocedural oesophageal stricture...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29231587/-clinical-study-of-post-stroke-upper-limb-spasmodic-hemiplegia-treated-with-jingou-diaoyu-needling-technique-and-bobath-therapy
#9
Runjie Sun, Liang Tian, Xiaoli Fang, Xiaozheng Du, Bowen Zhu, Zhongyang Song, Xuan Xu, Xiaoguang Qin
OBJECTIVE: To compare the difference in the clinical efficacy on post-stroke upper limb spasmodic hemiplegia between the combined therapy of jingou diaoyu needling technique and Bobath technology and simple Bobath technology. METHODS: Sixty patients were randomized into an observation group and a control group, 30 cases in each one. The usual medication of neurological internal medicine was used in the two groups. In the control group, Bobath facilitation technology was applied to the rehabilitation training...
April 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29231512/-discussion-on-dry-needling-being-part-of-acupuncture
#10
Zengfu Peng, Nenggui Xu, Zhaoxiang Bian, Canhui Li, Weidong Lu, Tao Huang, Shaobai Wang
We think that all the methods of puncturing into the skin to prevent and treat diseases are belong to acupuncture science. In spite of its basic theory of meridian and acupoint, anatomy and physiology have been important parts of modern acupuncture science. "Dry needling", however, is limited to trigger point theory. As for the positions, acupuncture is applied mainly at acupoints, involving in skin, muscles, tendons, vessels and nerves; while "dry needling" is used mostly at muscles. The needles of acupuncture are in various lengths and diameters and its manipulations are abundant, including the traditional skills and the achievements of modern science and technology research, such as electroacupuncture...
June 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29230413/establishment-and-application-of-prostate-cancer-circulating-tumor-cells-in-the-era-of-precision-medicine
#11
REVIEW
Yoon Seok Suh, Jae Young Joung, Sung Han Kim, Ho Kyung Seo, Jinsoo Chung, Kang Hyun Lee
Prostate cancer (PC) is the second most common cancer in men and is the fifth leading cause of cancer-related deaths worldwide. Additionally, there is concern for overdiagnosis and overtreatment of PC. Thus, selection of an appropriate candidate for active surveillance as well as more accurate and less invasive tools for monitoring advanced PC is required. Circulating tumor cells (CTCs) have emerged as a liquid biopsy tool; there have been several reports on its role, technologies, and applications to various cancers, including PC...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29230248/a-concise-review-on-the-use-of-mesenchymal-stem-cells-in-cell-sheet-based-tissue-engineering-with-special-emphasis-on-bone-tissue-regeneration
#12
REVIEW
A Cagdas Yorukoglu, A Esat Kiter, Semih Akkaya, N Lale Satiroglu-Tufan, A Cevik Tufan
The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29226748/epigenetic-biomarkers-current-strategies-and-future-challenges-for-their-use-in-the-clinical-laboratory
#13
José Luis García-Giménez, Marta Seco-Cervera, Trygve O Tollefsbol, Carlos Romá-Mateo, Lorena Peiró-Chova, Pablo Lapunzina, Federico V Pallardó
Epigenetic modifications and regulators represent potential molecular elements which control relevant physiological and pathological features, thereby contributing to the natural history of human disease. These epigenetic modulators can be employed as disease biomarkers, since they show several advantages and provide information about gene function, thus explaining differences among patient endophenotypes. In addition, epigenetic biomarkers can incorporate information regarding the effects of the environment and lifestyle on health and disease, and monitor the effect of applied therapies...
December 11, 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/29226660/bone-and-joint-diseases-in-present-and-future
#14
Yasuharu Nakashima, Ken Okazak, Koichiet Nakayama, Seiji Okada, Hideki Mizu-uchi
With the increase of elderly population, orthopaedic surgeons need to deal with the diseases related to aging, such as joint disorders and fragility fractures. The number of total joint replacements, for example, is two times more than it was 10 years ago. With these backgrounds, the Japanese Orthopaedic Association (JOA) has proposed the concept of locomotive syndrome; conditions under which the elderly have been receiving care services due to problems of the locomotive organs. To prevent geriatric or disuse syndrome, JOA is currently providing the care‒prevention programs such as the loco-check and loco-training...
January 2017: Fukuoka Igaku Zasshi, Hukuoka Acta Medica
https://www.readbyqxmd.com/read/29226111/comparison-of-electronic-learning-versus-lecture-based-learning-in-improving-emergency-medicine-residents-knowledge-about-mild-induced-hypothermia-after-cardiac-arrest
#15
Maryam Soleimanpour, Farzad Rahmani, Mehrad Naghizadeh Golzari, Alireza Ala, Hamid Reza Morteza Bagi, Robab Mehdizadeh Esfanjani, Hassan Soleimanpour
Background: The process of medical education depends on several issues such as training materials, students, professors, educational fields, and the applied technologies. The current study aimed at comparing the impacts of e-learning and lecture-based learning of mild induced hypothermia (MIH) after cardiac arrest on the increase of knowledge among emergency medicine residents. Methods: In a pre- and post-intervention study, MIH after cardiac arrest was taught to 44 emergency medicine residents...
August 2017: Anesthesiology and Pain Medicine
https://www.readbyqxmd.com/read/29225862/hitherto-unseen-survival-in-an-alk-positive-patient-with-advanced-stage-adult-ganglioneuroblastoma-treated-with-personalized-medicine
#16
Signe Risum, Ulrich Knigge, Seppo W Langer
Survival of stage 4 ganglioneuroblastoma (GNB) patients is poor; no reports exist of patients surviving up to 5 years (1, 2). We report the clinical and therapeutic course of a patient with stage 4 GNB surviving beyond expectations due to a multimodal treatment approach incorporating new technologies in cancer diagnostic and treatment.
December 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/29225665/evolution-and-clinical-translation-of-drug-delivery-nanomaterials
#17
Shabir Hassan, Gyan Prakash, Aycabal Ozturk, Saghi Saghazadeh, Mohammad Farhan Sohail, Jungmok Seo, Mehmet Dockmeci, Yu Shrike Zhang, Ali Khademhosseini
With the advent of technology, the role of nanomaterials in medicine has grown exponentially in the last few decades. The main advantage of such materials has been exploited in drug delivery applications, due to their effective targeting that in turn reduces systemic toxicity compared to the conventional routes of drug administration. Even though these materials offer broad flexibility based on targeting tissue, disease, and drug payload, the demand for more effective yet highly biocompatible nanomaterial-based drugs is increasing...
August 2017: Nano Today
https://www.readbyqxmd.com/read/29224409/intellectual-property-considerations-for-molecular-diagnostic-development-with-emphasis-on-companion-diagnostics
#18
Harry Glorikian, Richard Jeremy Warburg, Kelly Moore, Jennifer Malinowski
The development of molecular diagnostics is a complex endeavor, with multiple regulatory pathways to consider and numerous approaches to development and commercialization. Companion diagnostics, devices which are "essential for the safe and effective use of a corresponding drug or diagnostic product" (see U.S. Food & Drug Administration, In Vitro Diagnostics - Companion Diagnostics, U.S. Dept. of Health & Human Services(2016), available at https://www.fda.gov/medicaldevices/productsandmedicalprocedures/invitrodiagnostics/ucm407297...
December 9, 2017: Expert Opinion on Therapeutic Patents
https://www.readbyqxmd.com/read/29224260/-ten-years-retrospective-review-of-the-application-of-digital-medical-technology-in-general-surgery-in-china
#19
C H Fang, Y Y Lau, W P Zhou, W Cai
Digital medical technology is a powerful tool which has forcefully promoted the development of general surgery in China. In this article, we reviews the application status of three-dimensional visualization and three-dimensional printing technology in general surgery, introduces the development situation of surgical navigation guided by optical and electromagnetic technology and preliminary attempt to combined with mixed reality applied to complicated hepatectomy, looks ahead the development direction of digital medicine in the era of artificial intelligence and big data on behalf of surgical robot and radiomics...
December 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/29224259/-expert-consensus-of-precise-diagnosis-and-treatment-for-pancreatic-head-cancer-using-three-dimensional-visualization-technology
#20
(no author information available yet)
Three-dimensional (3D) visualization technology in pancreatic head cancers could offer decision-making support to preoperative diagnosis, resectability assessment and individualized surgical planning. In addition, the pancreas 3D printing helps to realize the leapfrog development from 3D images to 3D physical models and provides better guidance of the precise surgery of complicated cancers of the pancreatic head. In order to standardize the application of 3D visualization and 3D printing technology in the diagnosis and treatment of pancreatic head cancer, Chinese experts in relevant fields were organized by four committees to formulate this expert consensus...
December 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
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