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A H Reid, X Shen, P Maldonado, T Chase, E Jal, P W Granitzka, K Carva, R K Li, J Li, L Wu, T Vecchione, T Liu, Z Chen, D J Higley, N Hartmann, R Coffee, J Wu, G L Dakowski, W F Schlotter, H Ohldag, Y K Takahashi, V Mehta, O Hellwig, A Fry, Y Zhu, J Cao, E E Fullerton, J Stöhr, P M Oppeneer, X J Wang, H A Dürr
"The technical support from SLAC Accelerator Directorate, Technology Innovation Directorate, LCLS laser division and Test Facility Division is gratefully acknowledged. We thank S.P. Weathersby, R.K. Jobe, D. McCormick, A. Mitra, S. Carron and J. Corbett for their invaluable help and technical assistance. Research at SLAC was supported through the SIMES Institute which like the LCLS and SSRL user facilities is funded by the Office of Basic Energy Sciences of the U.S. Department of Energy under Contract No...
March 7, 2018: Nature Communications
Xiaoli Liu, Qixing Jiang, Wenshui Xia
The purpose of this work was to develop a nontoxic bioactive material based on a natural pyrone compound (kojic acid, KA) and chitosan oligosaccharides (COS). The bioactive material, chitosan oligosaccharide-N-kojic acid polymer (COS-N-KA), was prepared by one-step environmentally friendly approach. Then, the physicochemical properties and biological activities of COS-N-KA as a prepared water-soluble COS derivative were evaluated. The polymer was characterized by using UV-vis, FTIR,1 H NMR, and13 C NMR spectroscopy, Mw, PID, TGA, water solubility, hemolysis assay, and animal toxicity studies...
March 2, 2018: International Journal of Biological Macromolecules
Rebecca Woehl, Johannes Maier, Sebastian Gehmert, Christoph Palm, Birgit Riebschlaeger, Michael Nerlich, Michaela Huber
BACKROUND: Scaphoidectomy and midcarpal fusion can be performed using traditional fixation methods like K-wires, staples, screws or different dorsal (non)locking arthrodesis systems. The aim of this study is to test the Aptus four corner locking plate and to compare the clinical findings to the data revealed by CT scans and semi-automated segmentation. METHODS: This is a retrospective review of eleven patients suffering from scapholunate advanced collapse (SLAC) or scaphoid non-union advanced collapse (SNAC) wrist, who received a four corner fusion between August 2011 and July 2014...
February 13, 2018: BMC Musculoskeletal Disorders
Wei Quan, Chong Zhang, Meixia Zheng, Zhaoxin Lu, Fengxia Lu
BACKGROUND: The effects of Small laccase (SLAC) from Streptomyces coelicolor on the prosperities of whey protein isolate (WPI) film were studied. RESULTS: WPI was catalyze by SLAC without phenolic acid assistance. Particle size distribution results showed that some complexes with higher relative molecular weight formed in WPI samples treated by SLAC. The content of α-helixes decreased, while those of β-sheets and random coils increased following SLAC treatment according to circular dichroism results...
January 24, 2018: Journal of the Science of Food and Agriculture
Barbara Ramos, Daniel González-Acuña, David E Loyola, Warren E Johnson, Patricia G Parker, Melanie Massaro, Gisele P M Dantas, Marcelo D Miranda, Juliana A Vianna
BACKGROUND: Mitochondria play a key role in the balance of energy and heat production, and therefore the mitochondrial genome is under natural selection by environmental temperature and food availability, since starvation can generate more efficient coupling of energy production. However, selection over mitochondrial DNA (mtDNA) genes has usually been evaluated at the population level. We sequenced by NGS 12 mitogenomes and with four published genomes, assessed genetic variation in ten penguin species distributed from the equator to Antarctica...
January 16, 2018: BMC Genomics
Abdulhakeem B Ajibike, Oluwagbemiga O Adeleye, Babatunde M Ilori, Damilola A Osinbowale, Omolola A Adeniyi, Samuel O Durosaro, Adeyinka J Sanda, Oluwafunmilayo A Adebambo, Ayotunde O Adebambo
In this study, the maternal genetic diversity, phylogenetic relationship and effect of natural selection on indigenous chickens from Nigeria were assessed. A total of 397-bp fragment of the mitochondrial DNA (mtDNA) D-loop region of 171 indigenous chickens from four populations of Nigeria and four commercial egg line strains (two Anak titan, one Giriraja and one Yaffa) as out-groups were analysed. Thirty-one haplotypes (28 from Nigerian chickens and three from commercial strains) and 34 polymorphic sites were identified...
December 2017: Journal of Genetics
Ryan Cook, Drew Hannon, Jonathan N Southard, Sudipta Majumdar
A one semester undergraduate biochemistry laboratory experience is described for an understanding of recombinant technology from gene cloning to protein characterization. An integrated experimental design includes three sequential modules: molecular cloning, protein expression and purification, and protein analysis and characterization. Students perform the tasks of cloning, expression, purification, analysis, and characterization of small laccase (SLAC) from Streptomyces coelicolor. SLAC is an extremely robust well-characterized protein/enzyme, which serves as an ideal model for undergraduate teaching laboratories...
December 23, 2017: Biochemistry and Molecular Biology Education
Lisa Reissner, Stefanie Hensler, Sebastian Kluge, Miriam Marks, Daniel B Herren
PURPOSE: The objective was to investigate the clinical and subjective outcomes of patients after 4-corner arthrodesis (FCA) for scapholunate advanced collapse (SLAC) or scaphoid nonunion advanced collapse (SNAC) and to analyze complications. Furthermore, we compared the long-term results of a first-generation nonlocking plate (Spider) and a plate with a locking screw design (Flower plate). METHODS: In a retrospective cohort study, we included 39 patients with stage II or III SLAC or SNAC who underwent FCA...
December 14, 2017: Journal of Hand Surgery
Victor Bignatto Carvalho, Carlos Henrique Vieira Ferreira, Andresa Ramires Hoshino, Viviane Alves Bernardo, Gustavo Mantovani Ruggiero, Márcio Aurélio Aita
Objectives: To measure the quality of life, the time to work return, and clinical, functional, and radiographic parameters of patients treated with dorsal capsulodesis associated with scapholunate (SL) reconstruction, assisted by arthroscopy. Methods: From January 2015 to September 2016, 14 adult patients with SL dissociation underwent surgical treatment with the SL reconstruction procedure assisted by arthroscopy, using the new technique proposed in this study...
November 2017: Revista Brasileira de Ortopedia
Deepti Yadav, Bibhuti Ranjan, Nokuthula Mchunu, Marilize Le Roes-Hill, Tukayi Kudanga
This work reports for the first time the secretory expression of the small laccase (SLAC) from Streptomyces coelicolor A3(2) in Pichia pastoris. Using an AOX1 promoter and α factor as a secretion signal, the recombinant P. pastoris harbouring the laccase gene (rSLAC) produced high titres of extracellular laccase (500 ± 10 U/l), which were further increased seven fold by pre-incubation at 80 °C for 30 min. The enzyme (∼38 kDa) had an optimum activity at 80 °C, but optimum pH varied with substrate used...
March 2018: International Journal of Biological Macromolecules
Purnell Traverso, Anselm Wong, Ronit Wollstein, Lois Carlson, Duffield Ashmead, H Kirk Watson
BACKGROUND: Scapholunate advanced collapse (SLAC) of the wrist is the most common degenerative condition of the wrist. Four-corner fusion (4CF) is performed as salvage surgery, though there is limited information on its long-term results. We hypothesized that 4CF is a durable surgery with good clinical long-term function. METHODS: A retrospective chart review of patients undergoing 4CF as well as an interview and recent radiographs were obtained. Patients with a follow-up period of less than 10 years were excluded...
November 2017: Hand: Official Journal of the American Association for Hand Surgery
Xiaohu Luo, Ke Li, Jiali Xing, Lijun Qi, Ming Yang, Ren Wang, Li Wang, Yanan Li, Zhengxing Chen
Corn is an important food and feedstuff in China and worldwide. The problems caused by aflatoxin B1-contaminated corn (ACC) are of great concern. Our previous studies have demonstrated that ozone can effectively degrade AFB1 in corn, prompting us to investigate the in vivo toxicity of treated ACC. In this study, 35 Kunming mice were used to assess the in vivo toxicity of ozone treated ACC. Results indicated that compared to mice fed with basal feedstuff (provided by the Shanghai SLAC Laboratory), those fed with ACC have significantly decreased mean weight as well as total protein (TP), albumin (ALB), and globulin (GLB) contents (p<0...
October 23, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Enrico Giustiniano, Fabio Procopio, Guido Costa, Laura Rocchi, Nadia Ruggieri, Stefania Cantoni, Paola C Zito, Yari Gollo, Guido Torzilli, Ferdinando Raimondi
BACKGROUND: Serum lactate (sLac) concentration during liver resection with intermittent hepatic hilum clamping (i.e. Pringle maneuver, PM) was retrospectively investigated. METHODS: A total of 133 patients who underwent liver resection were enrolled. We analyzed the sLac peri-operatively. Correlations were searched between the PM and lactatemia and its variations (i.e. lactate clearance, cLac) and other factors which it might be related to. Lactatemia in triplicate intraoperatively was recorded, just after the awakening, and 1 and 2 h later...
November 2017: Journal of Hepato-biliary-pancreatic Sciences
T Zatrapa, D Veigl, J Pech, I Landor, M Šimková
PURPOSE OF THE STUDY Our study aimed to evaluate a group of patients who in the period from 2005 to 2014 underwent a four-corner arthrodesis of the wrist in our department. We also wanted to verify the hypothesis as to whether the use of conventional dorsal plate without the application of bone grafts leads to comparable results as the use of dorsal locking plates and routine application of bone grafts. MATERIAL AND METHODS Throughout the years 2005 to 2014 the four-corner arthrodesis of the wrist was performed in our department in a total of 62 patients, in two cases bilaterally...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
Ralf Bajinski, Joachim Gülke, Martin Mentzel, Florian Sigloch, Moritz Dustmann, Nikolaus Johannes Wachter
Scapholunate dissociation in stages 2 and 3 may lead to posttraumatic damage if left untreated. Different types of treatment have been described, including capsulodesis, tenodesis, and others. The purpose of all procedures is to avoid palmar shift of the distal scaphoid resulting in secondary arthritis (SLAC wrist). This study aimed to evaluate a modified dorsal ligamentoplasty in second- and third-degree scapholunate dissociation in a prospective study design. Furthermore, the study aimed to find out if there is a correlation between radiological and clinical parameters at follow-up and whether or not a SLAC wrist can be avoided...
August 2017: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
J-F Hernekamp, U Kneser, T Kremer, B Bickert
OBJECTIVE: Preservation of residual mobility and pain reduction in the wrist in advanced carpal collapse (scapholunate advanced collapse, SLAC or scaphoid nonunion advanced collapse, SNAC). INDICATIONS: Advanced osteoarthritis of the radiocarpal and intercarpal articulations, SLAC/SNAC stages 2-3. CONTRAINDICATIONS: Arthrotic alterations to the proximal joint surface of the lunate bone or the corresponding joint surface of the radius (lunate fossa)...
August 8, 2017: Operative Orthopädie und Traumatologie
B Chedal-Bornu, D Corcella, A Forli, F Moutet, M Bouyer
The aim of this study was to determine and analyze the functional and radiographic outcomes after proximal row carpectomy (PRC). We hypothesized that this surgery could restore wrist mobility and function in case of radiocarpal osteoarthritis or severe carpal trauma. Sixty-two patients who had undergone PRC were included in this study: 44 patients with wrist osteoarthritis (11 SNAC, 24 SLAC, 3 Kienböck's disease, 6 other) and 18 patients with severe carpal trauma. Each patient underwent clinical (pain, range of motion, grip strength, functional scores) and radiographic evaluations...
October 2017: Hand Surgery and Rehabilitation
T N Wistisen, R E Mikkelsen, U I Uggerhøj, U Wienands, T W Markiewicz, S Gessner, M J Hogan, R J Noble, R Holtzapple, S Tucker, V Guidi, A Mazzolari, E Bagli, L Bandiera, A Sytov
We report on the first experimental observations of quasichanneling oscillations, recently seen in simulations and described theoretically. Although above-barrier particles penetrating a single crystal are generally seen as behaving almost as in an amorphous substance, distinct oscillation peaks nevertheless appear for particles in that category. The quasichanneling oscillations were observed at SLAC National Accelerator Laboratory by aiming 20.35 GeV positrons and electrons at a thin silicon crystal bent to a radius of R=0...
July 14, 2017: Physical Review Letters
Rainer Hedrich, Dietmar Geiger
Contents 1 I. 1 II. 2 III. 5 IV. 8 V. 11 VI. 12 13 13 References 13 SUMMARY: Stomatal guard cells control leaf CO2 intake and concomitant water loss to the atmosphere. When photosynthetic CO2 assimilation is limited and the ratio of CO2 intake to transpiration becomes suboptimal, guard cells, sensing the rise in CO2 concentration in the substomatal cavity, deflate and the stomata close. Screens for mutants that do not close in response to experimentally imposed high CO2 atmospheres identified the guard cell-expressed Slowly activating anion channel, SLAC1, as the key player in the regulation of stomatal closure...
July 19, 2017: New Phytologist
Dengji Lou, Houping Wang, Gang Liang, Diqiu Yu
SNF 1-RELATED PROTEIN KINASE 2 (SnRK2) is a family of plant-specific protein kinases which is the key regulator of hyper-osmotic stress signaling and abscisic acid (ABA)-dependent development in various plants. Among the rice subclass-I and -II SnRK2s, osmotic stress/ABA-activated protein kinase 2 (SAPK2) may be the primary mediator of ABA signaling. However, SAPK2 has not been comprehensively characterized. In this study, we elucidated the functional properties of SAPK2 using loss-of-function mutants produced with the CRISPR/Cas9 system...
2017: Frontiers in Plant Science
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