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https://www.readbyqxmd.com/read/28821189/y-chromosome-markers-for-the-red-fox
#1
Halie M Rando, Jeremy T Stutchman, Estelle R Bastounes, Jennifer L Johnson, Carlos A Driscoll, Christina S Barr, Lyudmila N Trut, Benjamin N Sacks, Anna V Kukekova
The de novo assembly of the red fox (Vulpes vulpes) genome has facilitated the development of genomic tools for the species. Efforts to identify the population history of red foxes in North America have previously been limited by a lack of information about the red fox Y-chromosome sequence. However, a megabase of red fox Y-chromosome sequence was recently identified over 2 scaffolds in the reference genome. Here, these scaffolds were scanned for repeated motifs, revealing 194 likely microsatellites. Twenty-three of these loci were selected for primer development and, after testing, produced a panel of 11 novel markers that were analyzed alongside 2 markers previously developed for the red fox from dog Y-chromosome sequence...
September 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28820703/assessment-of-u-s-pediatrician-knowledge-of-toxocariasis
#2
Dana M Woodhall, Amanda P Garcia, Craig A Shapiro, Shequenta L Wray, Andi L Shane, Chitra S Mani, Kelly K Stimpert, LeAnne M Fox, Susan P Montgomery
Toxocariasis, one of a group of parasitic diseases known as neglected parasitic infections, is a disease caused by the larvae of two species of Toxocara roundworms, Toxocara canis, from dogs, and less commonly Toxocara cati, from cats. Although most infected individuals are asymptomatic, clinical manifestations may include fever, fatigue, coughing, wheezing, or abdominal pain (visceral toxocariasis) or vision loss, retina damage, or eye inflammation (ocular toxocariasis). To assess U.S. pediatrician knowledge of toxocariasis, we conducted an electronic survey of American Academy of Pediatrics members...
July 31, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820428/pharmacokinetic-analysis-of-an-oral-multicomponent-joint-dietary-supplement-phycox-%C3%A2-in-dogs
#3
Stephanie E Martinez, Ryan Lillico, Ted M Lakowski, Steven A Martinez, Neal M Davies
Despite the lack of safety, efficacy and pharmacokinetic (PK) studies, multicomponent dietary supplements (nutraceuticals) have become increasingly popular as primary or adjunct therapies for clinical osteoarthritis in veterinary medicine. Phycox(®) is a line of multicomponent joint support supplements marketed for joint health in dogs and horses. Many of the active constituents are recognized anti-inflammatory and antioxidant agents. Due to a lack of PK studies in the literature for the product, a pilot PK study of select constituents in Phycox(®) was performed in healthy dogs...
August 18, 2017: Pharmaceutics
https://www.readbyqxmd.com/read/28820381/possible-role-of-fish-as-transport-hosts-for-dracunculus-spp-larvae
#4
Christopher A Cleveland, Mark L Eberhard, Alec T Thompson, Stephen J Smith, Hubert Zirimwabagabo, Robert Bringolf, Michael J Yabsley
To inform Dracunculus medinensis (Guinea worm) eradication efforts, we evaluated the role of fish as transport hosts for Dracunculus worms. Ferrets fed fish that had ingested infected copepods became infected, highlighting the importance of recommendations to cook fish, bury entrails, and prevent dogs from consuming raw fish and entrails.
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28820279/mri-and-clinical-characteristics-of-suspected-cerebrovascular-accident-in-nine-cats
#5
Danielle E Whittaker, Randi Drees, Elsa Beltran
Objectives Cerebrovascular accidents (CVAs) are infrequently reported in cats. To date, clinical characteristics, including lesion localisation and MRI findings, have only been reported in two cats. The aim of the current study is to document MRI findings in cats presenting with CVAs over an 11 year period. Cases were reviewed according to initial clinical presentation, subsequent physical and neurological findings, predisposing systemic disease and short- and long-term (when available) outcome with a view to identifying any typical pattern in disease occurrence...
August 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28820132/prevalence-of-yersinia-enterocolitica-bioserotype-3-o-3-among-children-with-diarrhea-china-2010-2015
#6
Ran Duan, Junrong Liang, Jing Zhang, Yuhuang Chen, Jing Wang, Jing Tong, Bangcheng Guo, Wanfu Hu, Mingliu Wang, Jiayong Zhao, Chang Liu, Huijing Hao, Xin Wang, Huaiqi Jing
Yersinia enterocolitica is thought to not significantly contribute to diarrheal disease in China, but evidence substantiating this claim is limited. We determined the prevalence of Y. enterocolitica infection and strain types present among children <5 years of age with diarrhea in China. The overall prevalence of pathogenic isolates was 0.59%. Prevalence of pathogenic bioserotype 3/O:3 varied geographically. In this population, the presence of fecal leukocytes was a characteristic of Y. enterocolitica infection and should be used as an indication for microbiological diagnostic testing, rather than for the diagnosis of bacillary dysentery...
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28819692/molecular-characterization-of-atypical-antigenic-variants-of-canine-rabies-virus-reveals-its-reintroduction-by-wildlife-vectors-in-southeastern-mexico
#7
Fabiola Garcés-Ayala, Nidia Aréchiga-Ceballos, Joanna M Ortiz-Alcántara, Elizabeth González-Durán, Sandra I Pérez-Agüeros, Alfonso Méndez-Tenorio, Belem Torres-Longoria, Irma López-Martínez, Lucía Hernández-Rivas, José Alberto Díaz-Quiñonez, José Ernesto Ramírez-González
Rabies is an infectious viral disease that is practically always fatal following the onset of clinical signs. In Mexico, the last case of human rabies transmitted by dogs was reported in 2006 and canine rabies has declined significantly due to vaccination campaigns implemented in the country. Here we report on the molecular characterization of six rabies virus strains found in Yucatan and Chiapas, remarkably, four of them showed an atypical reaction pattern when antigenic characterization with a reduced panel of eight monoclonal antibodies was performed...
August 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28819502/pattern-and-outcome-of-dog-bite-injuries-among-children-in-ado-ekiti-southwest-nigeria
#8
Ezra Olatunde Ogundare, Oladele Simeon Olatunya, Isaac Oluwadare Oluwayemi, Adekoya Joshua Inubile, Adekunle Bamidele Taiwo, Oyinkansola Tolulope Agaja, Alfred Airemionkhale, Adewale Fabunmi
INTRODUCTION: Dog bites in humans are a major public health problem. Globally, millions of people are bitten by dogs but most of the fatal cases occur in children. There is paucity of data on dog bite related diseases among Nigerian children. Objectives: to determine the pattern of dog bite injuries and associated health problems among children seen at Ekiti State University Teaching Hospital. METHODS: This is a retrospective study on the clinical data of patients managed for dog bite related injuries between January 2010 and June 2014...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28819088/lissencephaly-in-a-pekingese
#9
Genya Shimbo, Michihito Tagawa, Eiji Oohashi, Masashi Yanagawa, Kazuro Miyahara
A 1-year-old neutered male Pekingese was presented for evaluation and further treatment of cluster seizures. The dog had behavioral abnormalities, and a prosencephalic lesion was suspected following neurological examination. The dog showed signs of learning difficulty. Magnetic resonance imaging of the brain revealed a remarkably smooth cerebral cortex with a reduced number of gyri, as well as a cystic lesion associated with the quadrigeminal cistern. A diagnosis of lissencephaly, concurrent with a quadrigeminal cisternal cyst, was made...
August 17, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28818583/timing-of-fertile-period-for-successful-pregnancy-in-american-bully-dogs
#10
Sang-Eun Hahn, Young-Kwang Jo, Yun-Kyong Jin, Goo Jang
Determination of the timing of the estrus cycle is essential for fertile mating. There are physiological variations among breeds, between bitches, and between cycles of the same bitch. If serial monitoring and many tools are applied, the exact moment of ovulation could be pin-pointed. However, it leads to time and costly difficulties. Progesterone concentrations during estrus cycles follow a specific pattern, and it is largely used in timing of fertile period. Although it has similar pattern in general, it is likely that breed-specific differences exist...
July 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28818115/the-use-of-genetic-markers-to-estimate-relationships-between-dogs-in-the-course-of-criminal-investigations
#11
Roberta Ciampolini, Francesca Cecchi, Isabella Spinetti, Anna Rocchi, Filippo Biscarini
OBJECTIVE: Attacks on humans by dogs in a pack, though uncommon, do happen, and result in severe, sometimes fatal, injuries. We describe the role that canine genetic markers played during the investigation of a fatal dog-pack attack involving a 50-year-old male truck driver in a parking lot in Tuscany (Italy). Using canine specific STR genetic markers, the local authorities, in the course of their investigations, reconstructed the genetic relationships between the dogs that caused the deadly aggression and other dogs belonging to the owner of the parking who, at the moment of the aggression, was located in another region of Italy...
August 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28818107/patellofemoral-kinematics-in-dogs-with-cranial-cruciate-ligament-insufficiency-an-in-vivo-fluoroscopic-analysis-during-walking
#12
Stanley E Kim, Geoffrey J Zann, Selena Tinga, Erica J Moore, Antonio Pozzi, Scott A Banks
BACKGROUND: Complete rupture of the cranial cruciate ligament (CrCL) in dogs causes profound disturbance to stifle joint biomechanics. The objective of this study was to characterize the effects of cranial cruciate ligament (CrCL) insufficiency on patellofemoral (PF) kinematics in dogs during walking. Ten client-owned dogs (20-40 kg) with natural unilateral complete CrCL rupture were included. Dogs underwent computed tomographic scans to create digital bone-models of the patella and femur...
August 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28818085/a-rare-case-of-acute-toxoplasmosis-in-a-stray-dog-due-to-infection-of-t-gondii-clonal-type-i-public-health-concern-in-urban-settings-with-stray-animals
#13
Sergio Migliore, Salvatore La Marca, Cristian Stabile, Vincenzo Di Marco Lo Presti, Maria Vitale
BACKGROUND: Typing of Toxoplasma gondii strains is important in epidemiological surveys, to understand the distribution and virulence of different clones of the parasite among human and animal populations. Stray dogs can be consider sentinel animals for contaminated environments playing an important but probably under- evaluated role in the epidemiology of T. gondii. We reported a rare case of acute toxoplasmosis in a stray dog due to clonal type I infection. The clonal type I, sporadic in Europe, is frequently associated with severe toxoplasmosis in humans and the control of its circulation is particularly relevant for public health...
August 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28818076/extensive-protein-hydrolyzation-is-indispensable-to-prevent-ige-mediated-poultry-allergen-recognition-in-dogs-and-cats
#14
Thierry Olivry, Jennifer Bexley, Isabelle Mougeot
BACKGROUND: The central premise for the commercialization of diets with hydrolyzed ingredients is that the small-sized digested peptides would be unable to crosslink allergen-specific IgE at the surface of tissue mast cells and induce their degranulation. Evidence for the validity of this concept to diagnose food allergies in dogs and cats is limited, however. Our objectives were to study the recognition of standard and variably hydrolyzed poultry extracts by sera from dogs and cats with elevated chicken-specific serum IgE...
August 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28817687/morphologic-phenotypic-and-transcriptomic-characterization-of-classically-and-alternatively-activated-canine-blood-derived-macrophages-in-vitro
#15
Franziska Heinrich, Annika Lehmbecker, Barbara B Raddatz, Kristel Kegler, Andrea Tipold, Veronika M Stein, Arno Kalkuhl, Ulrich Deschl, Wolfgang Baumgärtner, Reiner Ulrich, Ingo Spitzbarth
Macrophages are a heterogeneous cell population playing a pivotal role in tissue homeostasis and inflammation, and their phenotype strongly depends on the micromilieu. Despite its increasing importance as a translational animal model for human diseases, there is a considerable gap of knowledge with respect to macrophage polarization in dogs. The present study comprehensively investigated the morphologic, phenotypic, and transcriptomic characteristics of unstimulated (M0), M1- (GM-CSF, LPS, IFNγ-stimulated) and M2- (M-CSF, IL-4-stimulated)-polarized canine blood-derived macrophages in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28817629/maldi-tof-ms-identification-of-anopheles-gambiae-giles-blood-meal-crushed-on-whatman-filter-papers
#16
Sirama Niare, Lionel Almeras, Fatalmoudou Tandina, Amina Yssouf, Affane Bacar, Ali Toilibou, Ogobara Doumbo, Didier Raoult, Philippe Parola
BACKGROUND: Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals...
2017: PloS One
https://www.readbyqxmd.com/read/28815918/a-model-based-approach-to-characterize-individual-inbreeding-at-both-global-and-local-genomic-scales
#17
T Druet, M Gautier
Inbreeding results from the mating of related individuals and may be associated with reduced fitness because it brings together deleterious variants in one individual. In general, inbreeding is estimated with respect to an arbitrary base population consisting of ancestors that are assumed unrelated. We herein propose a model-based approach to estimate and characterize individual inbreeding at both global and local genomic scales by assuming the individual genome is a mosaic of HBD (Homozygous-By-Descent) and non-HBD segments...
August 16, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28815849/molecular-aspects-of-uterine-diseases-in-dogs
#18
REVIEW
R Hagman
Uterine diseases are common in dogs, particularly in countries where elective spaying is not usually performed. The associated clinical illnesses may be of varying degree ranging from merely decreased fertility to a critical pyometra requiring intensive care to survive. The diagnosis of some uterine diseases is generally uncomplicated, such as in a classic pyometra presenting with enlarged, fluid-filled uterus, purulent vaginal discharge and characteristic signs of illness or in other disorders associated with uterine enlargement...
August 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28815733/comparison-of-the-pharmacokinetic-profiles-of-two-different-amphotericin-b-formulations-in-healthy-dogs
#19
B Bingöl, T Bakirel
This study was conducted to compare the pharmacokinetic profiles of conventional (Fungizone(®) ) and liposomal amphotericin B (AmBisome(®) ) formulations in order to predict their therapeutic properties, and evaluate their potential differences in veterinary treatment. For this purpose, twelve healthy mixed breed dogs received both drugs at a dose of 0.6 mg/kg by intravenous infusion over a 4-min period in a total volume of 40 ml. Blood samples were collected at 0, 0.5, 1, 1.5, 2, 3, 4, 8, 12, 24, 48, 72 and 96 hr after dosing, and concentrations of drug in plasma were determined by high-performance liquid chromatography (HPLC)...
August 16, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28815682/molecular-evidence-for-trans-stadial-transmission-of-anaplasma-platys-by-rhipicephalus-sanguineus-sensu-lato-under-field-conditions
#20
M Aktas, S Ozubek
This study investigated the transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae). Engorged nymphs (n = 404) removed from A. platys-infected dogs were incubated at 28 °C until moulting. Unfed adults were obtained and divided into 48 pools. Unfed ticks collected from the grounds of the dog shelter, comprising 1800 larvae (n = 18 pools), 3100 nymphs (n = 62 pools) and 85 adults (n = 10 pools, including three male and seven female pools) were sorted into 90 pools. All pools were screened by polymerase chain reaction for the 16S rRNA gene of A...
August 16, 2017: Medical and Veterinary Entomology
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