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Bloodstream infection detection

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https://www.readbyqxmd.com/read/28542614/evaluation-of-the-sepsis-flow-chip-assay-for-the-diagnosis-of-blood-infections
#1
Antonio Galiana, Javier Coy, Adelina Gimeno, Noemi Marco Guzman, Francisco Rosales, Esperanza Merino, Gloria Royo, Juan Carlos Rodríguez
BACKGROUND: Blood infections are serious complex conditions that generally require rapid diagnosis and treatment. The big challenge is to reduce the time necessary to make a diagnosis with current clinical microbiological methods so as to improve the treatment given to patients. METHODS: In this study, we assess for the first time the Sepsis Flow Chip assay, which is a novel diagnostic assay for simultaneous rapid-detection of the vast majority of bloodstream pathogens, including Gram-positive and Gram-negative bacteria and fungi, in the same assay, and for the detection of most common antibiotic resistance genes...
2017: PloS One
https://www.readbyqxmd.com/read/28514306/unusual-case-of-rapid-growing-intraabdominal-abscess-caused-by-stenotrophomonas-maltophilia-after-laparoscopic-appendectomy-due-to-perforated-appendicitis-a-case-report
#2
Dae Ro Lim, Jung Cheol Kuk, Taehyung Kim, Eung Jin Shin
INTRODUCTION: An intraabdominal abscess due to Stenotrophomonas maltophilia (S maltophilia) infection is a very rare clinical manifestation. S maltophilia is a glucose nonfermentative, aerobic, gram-negative, mobile, and biofilm-forming bacterium. It is an opportunistic pathogen and uncommon cause of infection. Respiratory tract infections (pneumonia) and bloodstream infections (bacteremia) are the most common clinical manifestations of S maltophilia infection. CONCLUSIONS: This case report describes an unusual case of a rapidly growing, extremely large intraabdominal abscess (within 1 week during antibiotic therapy), which was detected 2 weeks after a laparoscopic appendectomy was performed for perforated appendicitis and was caused by multidrug-resistant S maltophilia infection...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28506673/high-frequency-of-silver-resistance-genes-in-invasive-isolates-of-enterobacter-and-klebsiella-species
#3
S Sütterlin, M Dahlö, C Tellgren-Roth, W Schaal, Å Melhus
BACKGROUND: Silver-based products have been marketed as an alternative to antibiotics, and their consumption has increased. Bacteria may, however, develop resistance to silver. AIM: To study the presence of genes encoding silver resistance (silE, silP, silS) over time in three clinically important Enterobacteriaceae genera. METHODS: Using polymerase chain reaction (PCR), 752 bloodstream isolates from the years 1990-2010 were investigated. Age, gender, and ward of patients were registered, and the susceptibility to antibiotics and silver nitrate was tested...
April 26, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28501098/alterations-in-intestinal-microbiota-relate-to-intestinal-failure-associated-liver-disease-and-central-line-infections
#4
Panliang Wang, Ying Wang, Lina Lu, Weihui Yan, Yijing Tao, Kejun Zhou, Jie Jia, Wei Cai
BACKGROUND: The gut microbiota plays a vital role in modulating the metabolic and immune functions of the intestines. We aimed to analyze the dysbiosis of microbiota in infants with short bowel syndrome (SBS) with different complications. PROCEDURE: We included 26 fecal samples from 18 infants with SBS during parenteral nutrition. The samples were categorized into three groups: asymptomatic, parenteral nutrition-associated liver disease (PNALD), and central line-associated bloodstream infection (CLABSI)...
May 3, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28498997/initiation-of-gametocytogenesis-at-very-low-parasite-density-in-plasmodium-falciparum-infection
#5
Ryan Farid, Matthew W Dixon, Leann Tilley, James S McCarthy
The recent focus on the elimination of malaria has led to an increased interest in the role of sexual stages in its transmission. We introduce Plasmodium falciparum gametocyte exported protein-5 (PfGEXP5) transcript analysis as an important tool for evaluating the earliest (ring) stage sexual gametocytes in the blood of infected individuals. We show that gametocyte rings are detected in the peripheral blood immediately following establishment of asexual infections-without the need for triggers such as high-density asexual parasitemia or drug treatment...
April 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28490008/an-uncommon-st1224-ndm-1-producing-klebsiella-pneumoniae-isolated-from-the-bloodstream-of-a-leukemia-patient-in-china
#6
Xiaoxiao Zhang, Jia Du, Cui Zhou, Jianming Cao, Hong Lu, Haiyang Liu, Wenzi Bi, Tieli Zhou
AIMS: This study aimed to analyze the clinical data and characteristics of an NDM-1 (New Delhi metallo-β-lactamase-1)-producing Klebsiella pneumoniae isolated from the bloodstream of a leukemia patient. MATERIALS AND METHODS: A retrospective analysis was used for the clinical data of the patient. The modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-disk synergy test were used for detecting metallo-β-lactamase. Antibiotic resistance was determined using the agar dilution method...
May 11, 2017: Chemotherapy
https://www.readbyqxmd.com/read/28481153/von-willebrand-factor-as-a-novel-player-in-valvular-heart-disease-from-bench-to-valve-replacement
#7
Felice Gragnano, Mario Crisci, Maurizio Cappelli Bigazzi, Renatomaria Bianchi, Simona Sperlongano, Francesco Natale, Fabio Fimiani, Claudia Concilio, Arturo Cesaro, Ivana Pariggiano, Vincenzo Diana, Giuseppe Limongelli, Plinio Cirillo, Mariagiovanna Russo, Enrica Golia, Paolo Calabrò
von Willebrand Factor (vWF) is a well-known mediator of hemostasis and vascular inflammation. Its dynamic modulation in the bloodstream, according to hemodynamic conditions, makes it an appealing biomarker in patients with valvular heart disease (VHD). Recent studies highlight the close connection between vWF and VHD, with possible implications in the pathogenesis of VHD, promoting valve aging and calcification or favoring the development of infective endocarditis. Moreover, vWF has been recently proposed as a new diagnostic and prognostic tool in patients with valve stenosis or regurgitation, showing a strict correlation with severity of valve disease, outcome, and bleeding (Heyde syndrome)...
January 1, 2017: Angiology
https://www.readbyqxmd.com/read/28472260/whole-genome-sequencing-accurately-identifies-resistance-to-extended-spectrum-%C3%AE-lactams-for-major-gram-negative-bacterial-pathogens
#8
Samuel A Shelburne, Jiwoong Kim, Jose M Munita, Pranoti Sahasrabhojane, Ryan K Shields, Ellen G Press, Xiqi Li, Cesar A Arias, Brandi Cantarel, Ying Jiang, Min S Kim, Samuel L Aitken, David E Greenberg
Background: There is marked interest in using DNA based methods to detect antimicrobial resistance (AMR) with targeted polymerase chain reaction (PCR) approaches increasingly being incorporated into clinical care. Whole genome sequencing (WGS) could offer significant advantages over targeted PCR for AMR detection, particularly for species where mutations are major drivers of AMR. Methods: Illumina MiSeq WGS and broth microdilution (BMD) assays were performed on 90 bloodstream isolates of the four most common gram-negative bacteria causing bloodstream infections in neutropenic patients...
May 3, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28449732/three-cases-of-mcr-1-positive-colistin-resistant-escherichia-coli-bloodstream-infections-in-italy-august-2016-to-january-2017
#9
Marta Corbella, Bianca Mariani, Carolina Ferrari, Francesco Comandatore, Erika Scaltriti, Piero Marone, Patrizia Cambieri
We describe three cases of bloodstream infection caused by colistin-resistant Escherichia coli in patients in a tertiary hospital in Italy, between August 2016 and January 2017. Whole genome sequencing detected the mcr-1 gene in three isolated strains belonging to different sequence types (STs). This occurrence of three cases with mcr-1-positive E. coli belonging to different STs in six months suggests a widespread problem in settings where high multidrug resistance is endemic such as in Italy.
April 20, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28448786/microbiological-features-and-clinical-impact-of-the-type-vi-secretion-system-t6ss-in-acinetobacter-baumannii-isolates-causing-bacteremia
#10
Jungok Kim, Ji-Young Lee, Haejeong Lee, Ji Young Choi, Dae Hun Kim, Yu Mi Wi, Kyong Ran Peck, Kwan Soo Ko
We investigated the genetic background and microbiological features of T6SS-positive Acinetobacter baumannii isolates and clinical impact of the T6SS in patients with A. baumannii bacteremia. One hundred and sixty-two A. baumannii isolates from patients with bacteremia in two tertiary-care hospitals in Korea were included in this study. Approximately one-third (51/162, 31.5%) of the A. baumannii clinical isolates possessed the hcp gene, and the hcp-positive isolates were found in several genotypes in multilocus sequence typing...
April 27, 2017: Virulence
https://www.readbyqxmd.com/read/28448572/clonality-virulence-determinants-and-profiles-of-resistance-of-clinical-acinetobacter-baumannii-isolates-obtained-from-a-spanish-hospital
#11
Elias Dahdouh, Rosa Gómez-Gil, Sonsoles Pacho, Jesús Mingorance, Ziad Daoud, Monica Suárez
INTRODUCTION: Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. baumannii isolated from a Spanish hospital in terms of antimicrobial susceptibility, clonality, carbapenemase genes harbored, and virulence determinants expressed...
2017: PloS One
https://www.readbyqxmd.com/read/28446572/evaluation-of-the-accelerate-pheno%C3%A2-system-for-fast-identification-and-antimicrobial-susceptibility-testing-from-positive-blood-culture-in-gram-negative-bloodstream-infection
#12
Matthias Marschal, Johanna Bachmaier, Ingo Autenrieth, Philipp Oberhettinger, Matthias Willmann, Silke Peter
Bloodstream infections (BSI) are an important cause of morbidity and mortality. Increasing rates of antimicrobial resistant pathogens limit treatment options, prompting an empirical use of broad-range antibiotics. Fast and reliable diagnostic tools are needed to provide adequate therapy in a timely manner and to enable a de-escalation of treatment.The Accelerate Pheno™ system (Accelerate Diagnostics, USA) is a fully automated test system that performs both identification (ID) and antimicrobial susceptibility testing (AST) directly from positive blood cultures within approx...
April 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28442767/direct-rapid-antimicrobial-susceptibility-test-from-positive-blood-cultures-based-on-microscopic-imaging-analysis
#13
Jungil Choi, Hyun Yong Jeong, Gi Yoon Lee, Sangkwon Han, Shinhun Han, Bonghwan Jin, Taegeun Lim, Shin Kim, Dong Young Kim, Hee Chan Kim, Eui-Chong Kim, Sang Hoon Song, Taek Soo Kim, Sunghoon Kwon
For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28433579/distinguishing-septic-from-normal-donors-by-detection-of-spla2-iia-from-human-plasma-using-a-microsieve-based-immunoassay
#14
Daniel R Zweitzig, Arjan G Tibbe, Mark J Kopnitsky, Kathleen Cichonski, Leon W M M Terstappen
Bloodstream infections that progress to septic shock are responsible for hundreds of thousands of deaths each year, and are associated with significant healthcare costs. Recent studies have shown that a member of the secreted phospholipase protein family, termed sPLA2-IIA, may play a role during the innate immune response to bacterial infections, and is elevated in the plasma of septic patients. In this report, the feasibility of a simple microsieve-based sPLA2-IIA detection immunoassay was explored. Microsieves containing 5μm pores were covalently coupled with a sPLA2-IIA-specific monoclonal antibody at 0...
April 19, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28427796/molecular-methods-for-septicemia-diagnosis
#15
Francesc Marco
Septicemia remains a major cause of hospital mortality. Blood culture remains the best approach to identify the etiological microorganisms when a bloodstream infection is suspected but it takes long time because it relies on bacterial or fungal growth. The introduction in clinical microbiology laboratories of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology, DNA hybridization, microarrays or rapid PCR-based test significantly reduce the time to results. Tests for direct detection in whole blood samples are highly desirable because of their potential to identify bloodstream pathogens without waiting for blood cultures to become positive...
April 17, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28397472/comparison-of-novel-blood-culture-system-dl-bt112tm-with-bact-alert-3dtm-by-using-clinical-samples
#16
Mustafa Altindis, Tayfur Demiray, Mehmet Koroglu, Umit Kilic, Aybala Neslihan Alagoz, Aziz Ogutlu, Oguz Karabay
BACKGROUND: Blood cultures are the main diagnostic laboratory tool to detect bloodstream infections. Many clinical microbiology laboratories utilize automated blood culture systems to isolate infectious agents from blood samples. The diagnostic performance and time-to-detection values of the novel automated blood culture system, DLBt112TM (DL), was compared with BacT/Alert 3DTM (B3D) in this prospective comparative study with clinical samples. METHODS: A total of 356 blood culture sets (178 sets for each system) were evaluated over a 6-month period in a university hospital...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397261/validity-and-reliability-of-administrative-coded-data-for-the-identification-of-hospital-acquired-infections-an-updated-systematic-review-with-meta-analysis-and-meta-regression-analysis
#17
Olga Redondo-González, José María Tenías, Ángel Arias, Alfredo J Lucendo
OBJECTIVE: To conduct an updated assessment of the validity and reliability of administrative coded data (ACD) in identifying hospital-acquired infections (HAIs). METHODS: We systematically searched three libraries for studies on ACD detecting HAIs compared to manual chart review. Meta-analyses were conducted for prosthetic and nonprosthetic surgical site infections (SSIs), Clostridium difficile infections (CDIs), ventilator-associated pneumonias/events (VAPs/VAEs) and non-VAPs/VAEs, catheter-associated urinary tract infections (CAUTIs), and central venous catheter-related bloodstream infections (CLABSIs)...
April 11, 2017: Health Services Research
https://www.readbyqxmd.com/read/28395861/dissemination-and-clinical-implications-of-multidrug-resistant-klebsiella-pneumoniae-isolates-producing-oxa-48-in-a-spanish-hospital
#18
A Madueño, J González García, S Fernández-Romero, J Oteo, M Lecuona
BACKGROUND: Healthcare-associated infections caused by Klebsiella pneumoniae isolates are increasing and few effective antibiotics are currently available to treat patients. AIM: To assess the epidemiology, molecular basis, clinical features, and outcomes in the acquisition and dissemination of OXA-48-producing K. pneumoniae (OXA48KP) in a tertiary Spanish hospital between October 2013 and December 2015. METHODS: Clinical, demographic, and microbiological data of patients with OXA48KP in clinical samples were collected from medical records...
March 3, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28377868/a-case-of-breakthrough-candida-parapsilosis-fungemia-during-micafungin-therapy-for-a-candida-glabrata-bloodstream-infection
#19
Yuta Norimatsu, Daiichi Morii, Asako Kogure, Taeko Hamanaka, Yoshihiro Kuwano, Takayuki Yokozawa, Toshimi Oda
We describe a case of breakthrough Candida parapsilosis fungemia in an 80-year-old woman with pyoderma gangrenosum and rheumatoid arthritis. C. parapsilosis was detected in blood culture while the patient was treated with micafungin for a Candida glabrata bloodstream infection. The breakthrough infection was successfully treated with liposomal amphotericin B.
June 2017: Medical Mycology Case Reports
https://www.readbyqxmd.com/read/28369408/wgs-based-surveillance-of-third-generation-cephalosporin-resistant-escherichia-coli-from-bloodstream-infections-in-denmark
#20
Louise Roer, Frank Hansen, Martin Christen Frølund Thomsen, Jenny Dahl Knudsen, Dennis Schrøder Hansen, Mikala Wang, Jurgita Samulioniené, Ulrik Stenz Justesen, Bent L Røder, Helga Schumacher, Claus Østergaard, Leif Percival Andersen, Esad Dzajic, Turid Snekloth Søndergaard, Marc Stegger, Anette M Hammerum, Henrik Hasman
Objectives: To evaluate a genome-based surveillance of all Danish third-generation cephalosporin-resistant Escherichia coli (3GC-R Ec ) from bloodstream infections between 2014 and 2015, focusing on horizontally transferable resistance mechanisms. Methods: A collection of 552 3GC-R Ec isolates were whole-genome sequenced and characterized by using the batch uploader from the Center for Genomic Epidemiology (CGE) and automatically analysed using the CGE tools according to resistance profile, MLST, serotype and fimH subtype...
March 22, 2017: Journal of Antimicrobial Chemotherapy
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