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https://www.readbyqxmd.com/read/29353443/role-of-fluorescent-in-situ-hybridization-cholangioscopic-biopsies-and-eus-fna-in-the-evaluation-of-biliary-strictures
#1
Christian Brooks, Valerie Gausman, Chanthel Kokoy-Mondragon, Khushboo Munot, Sunil P Amin, Amit Desai, Claudine Kipp, John Poneros, Amrita Sethi, Frank G Gress, Michel Kahaleh, Vundavalli V Murty, Reem Sharaiha, Tamas A Gonda
BACKGROUND AND AIMS: Our goal was to compare the diagnostic accuracy of FISH in the detection of malignancy compared with other standard diagnostic modalities, including brush cytology and biopsy specimens over a 10-year period of prospective data collection. METHODS: We conducted a review of all consecutive biliary strictures evaluated between 2006 and 2016. Patients with a final pathologic diagnosis or conclusive follow-up were included. We evaluated the performance of FISH polysomy (CEP 3, 7, and 17) and 9p21 deletion as well as cholangioscopic biopsy (CBx) and EUS-FNA...
January 20, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29351090/parasitic-infections-of-the-skin-and-subcutaneous-tissues
#2
Andrew P Norgan, Bobbi S Pritt
A variety of arthropods, protozoa, and helminths infect the skin and subcutaneous tissues and may be identified by anatomic pathologists in standard cytology and histology preparations. The specific organisms seen vary greatly with the patient's exposure history, including travel to or residence in endemic countries. Arthropods are the most commonly encountered parasites in the skin and subcutaneous tissues and include Sarcoptes scabei, Demodex species, Tunga penetrans, and myiasis-causing fly larvae. Protozoal parasites such as Leishmania may also be common in some settings...
January 18, 2018: Advances in Anatomic Pathology
https://www.readbyqxmd.com/read/29350424/prevalence-of-high-grade-cervical-intraepithelial-neoplasia-in-women-with-persistent-high-risk-hpv-genotypes-and-negative-cytology
#3
M Macdonald, J Crossley, K Ellis, N Dudding, R Lyon, J H F Smith, J A Tidy, J E Palmer
INTRODUCTION: Primary HPV screening will be implemented into the English Cervical Screening Programme by 2019. Its impact upon women referred to colposcopy, with negative cytology but persistently positive high-risk HPV (hrHPV), remains unreported from UK Sentinel sites. HPV primary screening was introduced in Sheffield, UK in April 2013; this paper reports its impact on the service. METHODS: A retrospective cohort study was performed from June 2014 to July 2016 at the Jessop Wing Colposcopy Unit, Sheffield...
January 19, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/29350311/shear-wave-elastography-and-afirma%C3%A2-gene-expression-classifier-in-thyroid-nodules-with-indeterminate-cytology-a-comparison-study
#4
Ghobad Azizi, James M Keller, Michelle L Mayo, Kelé Piper, David Puett, Karly M Earp, Carl D Malchoff
PURPOSE: To compare shear wave elastography (SWE) and Afirma™ gene expression classifier (GEC) for diagnosis of malignancy in thyroid nodules (TNs) with Bethesda Classification (BC) III or IV indeterminate cytology. METHODS: This preliminary single-center prospective study was approved by the Institutional Review Board. We evaluated 151 consented patients with 151 indeterminate TNs (123 BC III, 28 BC IV) on fine-needle aspiration biopsy (FNAB). B-mode ultrasound, vascularity, and SWE were performed prior to FNAB...
January 19, 2018: Endocrine
https://www.readbyqxmd.com/read/29349795/quality-of-life-assumptions-determine-which-cervical-cancer-screening-strategies-are-cost-effective
#5
Inge M C M de Kok, Ida J Korfage, Wilbert B van den Hout, Theo J M Helmerhorst, J Dik F Habbema, Marie-Louise Essink-Bot, Marjolein van Ballegooijen
Quality adjusted life years are used in cost-effectiveness analyses (CEAs). To calculate QALYs, a 'utility' (0-1) is used for each health state induced or prevented by the intervention. We aimed to estimate the impact of quality-of-life (QoL) assumptions (utilities and durations of health states) on CEAs of cervical cancer screening. To do so, twelve alternative sets of utility assumptions were retrieved from published cervical cancer screening CEAs. Two additional sets were based on empirical QoL data that were integrally obtained through two different measures (SF-6D and EQ-5D) from eight groups of women (total n= 3,087), from invitation for screening to diagnosis with cervical cancer...
January 19, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29348388/disseminated-histoplasmosis-in-an-indonesian-hiv-positive-patient-a-case-diagnosed-by-fine-needle-aspiration-cytology
#6
Nungki Anggorowati, Rita C Sulistyaningsih, Ahmad Ghozali, Yanri W Subronto
A 30-year-old Javanese-Indonesian man was admitted with complaints of 3 months persistent fever, weight loss, and fatigue. He had never known his past history of unprotected HIV until the admission. His only risk factor is unsafe sex. The patient seemed well nourished. Physical examination revealed blood pressure 100/60 mmHg, pulse 100 beats per minute, respiratory rate 20 times per minute, and temperature 38.8°C. Multiple cervical and inguinal lymphadenopathies were also found. Electrocardiogram showed anterolateral ischemic finding, whereas chest X-ray were normal...
October 2017: Acta Medica Indonesiana
https://www.readbyqxmd.com/read/29347883/pulmonary-laser-metastasectomy-by-1318-nm-neodymium-doped-yttrium-aluminum-garnet-laser-a-retrospective-study-about-laser-metastasectomy-of-the-lung
#7
Calogero Porrello, Roberto Gullo, Antonino Vaglica, Gregorio Scerrino, Giuseppe Salamone, Leo Licari, Cristina Raspanti, Eliana Gulotta, Gaspare Gulotta, Gianfranco Cocorullo
BACKGROUND: The lungs are among the first organ affected by remote metastases from many primary tumors. The surgical resection of isolated pulmonary metastases represents an important and effective element of therapy. This is a retrospective study about our entire experience with pulmonary resection for metastatic cancer using 1318-nm neodymium-doped yttrium-aluminum garnet laser. METHOD: In this single-institution study, we retrospectively analyzed a group of 209 patients previously treated for primary malignant solid tumors...
January 1, 2018: Surgical Innovation
https://www.readbyqxmd.com/read/29346217/pathological-and-molecular-aspects-to-improve-endoscopic-ultrasonography-guided-fine-needle-aspiration-from-solid-pancreatic-lesions
#8
Hiroyuki Matsubayashi, Keiko Sasaki, Sachiyo Ono, Masato Abe, Hirotoshi Ishiwatari, Akira Fukutomi, Katsuhiko Uesaka, Hiroyuki Ono
Endoscopic ultrasonography-guided fine-needle aspiration (EUS-FNA) has been applied to pancreatic lesions since the 1990s, and its use is now widespread. Improvements in endoscopic devices and sampling techniques have resulted in excellent diagnostic ability for solid pancreatic lesions. However, clinical improvements alone are not responsible for it; pathological aspects have also played important roles. Rapid on-site evaluation minimizes endoscopic procedures, although its value at improving the diagnostic ratio is still debated...
February 2018: Pancreas
https://www.readbyqxmd.com/read/29346046/ultrasonographic-characteristics-of-the-reproductive-tract-and-serum-progesterone-and-estradiol-concentrations-in-captive-female-red-wolves-canis-rufus-with-and-without-reproductive-tract-disease
#9
Kadie M Anderson, Mandi W Schook, Karen L Goodrowe, William T Waddell, Karen N Wolf
OBJECTIVE To describe ultrasonographic characteristics of the reproductive tract and serum progesterone and estradiol concentrations in captive female red wolves (Canis rufus) with and without reproductive tract disease. DESIGN Prospective study. ANIMALS 13 adult female red wolves. PROCEDURES Wolves with varying parity and history of contraceptive treatment were anesthetized to facilitate ultrasonographic examination and measurement of the reproductive tract and blood collection for determination of serum progesterone and estradiol concentrations in December 2011 and June 2012...
February 1, 2018: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/29345728/analytical-performance-of-the-thyroseq-v3-genomic-classifier-for-cancer-diagnosis-in-thyroid-nodules
#10
Marina N Nikiforova, Stephanie Mercurio, Abigail I Wald, Michelle Barbi de Moura, Keith Callenberg, Lucas Santana-Santos, William E Gooding, Linwah Yip, Robert L Ferris, Yuri E Nikiforov
BACKGROUND: Molecular tests have clinical utility for thyroid nodules with indeterminate fine-needle aspiration (FNA) cytology, although their performance requires further improvement. This study evaluated the analytical performance of the newly created ThyroSeq v3 test. METHODS: ThyroSeq v3 is a DNA- and RNA-based next-generation sequencing assay that analyzes 112 genes for a variety of genetic alterations, including point mutations, insertions/deletions, gene fusions, copy number alterations, and abnormal gene expression, and it uses a genomic classifier (GC) to separate malignant lesions from benign lesions...
January 18, 2018: Cancer
https://www.readbyqxmd.com/read/29345551/quantifying-novice-and-expert-differences-in-visual-diagnostic-reasoning-in-veterinary-pathology-using-eye-tracking-technology
#11
Amy L Warren, Tyrone L Donnon, Catherine R Wagg, Heather Priest, Nicole J Fernandez
Visual diagnostic reasoning is the cognitive process by which pathologists reach a diagnosis based on visual stimuli (cytologic, histopathologic, or gross imagery). Currently, there is little to no literature examining visual reasoning in veterinary pathology. The objective of the study was to use eye tracking to establish baseline quantitative and qualitative differences between the visual reasoning processes of novice and expert veterinary pathologists viewing cytology specimens. Novice and expert participants were each shown 10 cytology images and asked to formulate a diagnosis while wearing eye-tracking equipment (10 slides) and while concurrently verbalizing their thought processes using the think-aloud protocol (5 slides)...
January 18, 2018: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/29344852/laser-capture-microdissection-insights-into-methods-and-applications
#12
Meera Mahalingam
Laser capture microdissection is a non-molecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. Its advantages include efficient rapid and precise procurement of cells. The potential disadvantages include time consuming, expensive, and limited by the need for a pathologist for recognition of distinct subpopulations within a specified sample. Overall it is versatile allowing the preparation of homogenous isolates of specific subpopulations of cells from which DNA/RNA or protein can be extracted for RT-PCR, quantitative PCR, next-generation sequencing, immunoblot blot analyses, and mass spectrometry...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344564/endoscopic-ultrasound-guided-needle-based-confocal-laser-endomicroscopy-in-solid-pancreatic-masses-a-prospective-validation-study
#13
John Gásdal Karstensen, Tatiana Cârţână, Codruţa Constantinescu, Silviu Dumitrașcu, Bojan Kovacevic, Pia Klausen, Hazem Hassan, Tobias Wirenfeldt Klausen, Helga Bertani, Manoop S Bhutani, Adrian Săftoiu, Peter Vilmann
Background and study aims:  Endoscopic ultrasound fine-needle aspiration (EUS-FNA) is a keystone in diagnosing and staging of pancreatic masses. Recently, a microfiber that can pass through a 19-gauge needle has been introduced for confocal laser endomicroscopy (nCLE). The aims of this study were to evaluate the diagnostic value and the reproducibility of nCLE criteria for solid malignant lesions. Patients and methods:  This prospective dual-center study included patients with pancreatic masses suspicious of malignancy referred for EUS-FNA...
January 2018: Endoscopy International Open
https://www.readbyqxmd.com/read/29344261/t-cell-acute-lymphoblastic-lymphoma-complicated-with-myeloid-sarcoma-in-an-adult-a-case-report
#14
Ying Wang, Shupeng Wen, Zhiyun Niu, Lina Xing, Fuxu Wang, Xuejun Zhang
The present case report describes a rare case of T cell acute lymphoblastic lymphoma (T-LBL) in the lymph node with myeloid sarcoma in the pericardium. A 33-year-old Chinese male was admitted to hospital on 4 July 2015 exhibiting a fever and having experienced wheezing and fatigue for the previous 7 days. Routine pathological, computed tomographic, cytological and immunophenotypic observations revealed a diagnosis of T-LBL in the lymph node on 7 August 2015, without evidence of bone marrow (BM) involvement...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344211/prevalence-of-hpv-infection-among-hiv-positive-and-hiv-negative-women-in-central-eastern-italy-strategies-of-prevention
#15
Edoardo Tartaglia, Katia Falasca, Jacopo Vecchiet, Giovanna Paola Sabusco, Giovanna Picciano, Roberto Di Marco, Claudio Ucciferri
The present cross-sectional-study aimed to determine the prevalence of human papillomavirus (HPV)-genotypes among human immunodeficiency virus (HIV)-positive and -negative women in Central/Eastern Italy, and to identify the optimal strategies for effective HPV-prevention in each group. A representative sample of HIV-negative (150/200) and -positive (50/200) women, who underwent cervico-vaginal-swabbing. Swabs were analysed for a cytological screening and for a HPV-DNA-genotyping-test. A total of 66/200 swabs resulted HPV-positive...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29343623/identifying-dna-methylation-biomarkers-for-non-endoscopic-detection-of-barrett-s-esophagus
#16
Helen R Moinova, Thomas LaFramboise, James D Lutterbaugh, Apoorva Krishna Chandar, John Dumot, Ashley Faulx, Wendy Brock, Omar De la Cruz Cabrera, Kishore Guda, Jill S Barnholtz-Sloan, Prasad G Iyer, Marcia I Canto, Jean S Wang, Nicholas J Shaheen, Prashanti N Thota, Joseph E Willis, Amitabh Chak, Sanford D Markowitz
We report a biomarker-based non-endoscopic method for detecting Barrett's esophagus (BE) based on detecting methylated DNAs retrieved via a swallowable balloon-based esophageal sampling device. BE is the precursor of, and a major recognized risk factor for, developing esophageal adenocarcinoma. Endoscopy, the current standard for BE detection, is not cost-effective for population screening. We performed genome-wide screening to ascertain regions targeted for recurrent aberrant cytosine methylation in BE, identifying high-frequency methylation within the CCNA1 locus...
January 17, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29343306/nasal-changes-associated-with-exercise-in-athletes-systematic-review
#17
P Surda, A Walker, J Limpens, W Fokkens, M Putala
BACKGROUND: The prevalence of rhinitis in athletes has frequently been studied in combination with asthma, but the impact of exercise on the paracrine and secretory functions of nasal mucosa is less well established. This systematic review aimed to examine the effect of exercise on nasal mucosa in elite athletes. METHOD: A systematic search of Medline, Embase and the non-Medline subset of PubMed, from inception to 8th March 2016, was performed to identify studies on rhinitis in athletes...
January 18, 2018: Journal of Laryngology and Otology
https://www.readbyqxmd.com/read/29343212/clinical-characteristics-of-subtypes-of-follicular-variant-papillary-thyroid-carcinoma
#18
Min Joo Kim, Jae-Kyung Won, Kyeong Cheon Jung, Ji-Hoon Kim, Sun Wook Cho, Do Joon Park, Young Joo Park
Background Among follicular variant papillary thyroid carcinomas (FVPTCs), the non-invasive encapsulated subtype has an excellent prognosis. For this reason, re-classification of non-invasive encapsulated FVPTC (EFVPTC) as a new entity called "non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP)" has been proposed, but controversy remains. To characterize non-invasive EFVPTC in an Asian population, we compared the clinicopathological features of each FVPTC subtype in a Korean population...
January 17, 2018: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/29341505/stability-integrity-and-recovery-rate-of-cellular-nucleic-acids-preserved-in-a-new-liquid-based-cytology-medium
#19
Guodong Zhao, Zhaocheng Liu, Yaping Tian, Minsheng Zhu, Shouli Wang, Hui Wang, Shen Gao, Yong Ma, Minxue Zheng
BACKGROUND: Liquid-based cytology (LBC) has replaced the conventional Papanicolaou test in cervical cancer screening. The cervical swab specimens collected in LBC media can also be used for additional analyses including high-risk HPV (HR-HPV) test, DNA methylation analysis, and HPV E6/E7 mRNA test. METHODS: The stability, integrity, and recovery rate of cellular DNA and RNA after storage at different conditions were evaluated by a quantitative real-time PCR (qPCR) based HR-HPV test, reverse transcription qPCR (RT-qPCR), and agarose gel electrophoresis...
January 16, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29341477/evidence-based-adequacy-criteria-for-instrumented-urine-cytology-using-cytospin-preparations
#20
Andrew A Renshaw, Edwin W Gould
Cytospin preparations of instrumented urine cytology specimens with less than 10 urothelial cells or more than 50 urothelial cells/10 hpfs are both associated with significantly increased false negative rates compared to cases with 10-49 urothelial cells/10 hpfs.
January 16, 2018: Diagnostic Cytopathology
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