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https://www.readbyqxmd.com/read/28938120/prion-like-domains-program-ewing-s-sarcoma
#1
James Shorter
Prion-like domains have emerged as important drivers of neurodegenerative disease. Now, Boulay et al. establish that the translocated prion-like domain of the oncogenic EWS-FLI1 fusion protein enables phase-separation events, which inappropriately recruit chromatin-remodeling factors to elicit the aberrant transcriptional programs underlying Ewing's sarcoma.
September 21, 2017: Cell
https://www.readbyqxmd.com/read/28938114/the-eukaryotic-co2-concentrating-organelle-is-liquid-like-and-exhibits-dynamic-reorganization
#2
Elizabeth S Freeman Rosenzweig, Bin Xu, Luis Kuhn Cuellar, Antonio Martinez-Sanchez, Miroslava Schaffer, Mike Strauss, Heather N Cartwright, Pierre Ronceray, Jürgen M Plitzko, Friedrich Förster, Ned S Wingreen, Benjamin D Engel, Luke C M Mackinder, Martin C Jonikas
Approximately 30%-40% of global CO2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly...
September 21, 2017: Cell
https://www.readbyqxmd.com/read/28938112/non-coding-transcription-instructs-chromatin-folding-and-compartmentalization-to-dictate-enhancer-promoter-communication-and-t-cell-fate
#3
Takeshi Isoda, Amanda J Moore, Zhaoren He, Vivek Chandra, Masatoshi Aida, Matthew Denholtz, Jan Piet van Hamburg, Kathleen M Fisch, Aaron N Chang, Shawn P Fahl, David L Wiest, Cornelis Murre
It is now established that Bcl11b specifies T cell fate. Here, we show that in developing T cells, the Bcl11b enhancer repositioned from the lamina to the nuclear interior. Our search for factors that relocalized the Bcl11b enhancer identified a non-coding RNA named ThymoD (thymocyte differentiation factor). ThymoD-deficient mice displayed a block at the onset of T cell development and developed lymphoid malignancies. We found that ThymoD transcription promoted demethylation at CTCF bound sites and activated cohesin-dependent looping to reposition the Bcl11b enhancer from the lamina to the nuclear interior and to juxtapose the Bcl11b enhancer and promoter into a single-loop domain...
September 21, 2017: Cell
https://www.readbyqxmd.com/read/28937772/terahertz-light-matter-interaction-beyond-unity-coupling-strength
#4
Andreas Bayer, Marcel Pozimski, Simon Schambeck, Dieter Schuh, Rupert Huber, Dominique Bougeard, Christoph Lange
Achieving control over light-matter interaction in custom-tailored nanostructures is at the core of modern quantum electrodynamics. In strongly and ultrastrongly coupled systems, the excitation is repeatedly exchanged between a resonator and an electronic transition at a rate known as the vacuum Rabi frequency ΩR. For ΩR approaching the resonance frequency ωc, novel quantum phenomena including squeezed states, Dicke superradiant phase transitions, the collapse of the Purcell effect, and a population of the ground state with virtual photon pairs are predicted...
September 22, 2017: Nano Letters
https://www.readbyqxmd.com/read/28937745/mononuclear-fe-ii-complexes-based-on-the-methylpyrazinyl-diamine-ligand-chemical-thermo-and-photocontrol-of-their-magnetic-switchability
#5
Xue Liu, Jian Zhou, Xin Bao, Zheng Yan, Guo Peng, Mathieu Rouzières, Corine Mathonière, Jun-Liang Liu, Rodolphe Clérac
Two new mononuclear Fe(II) complexes, [Fe((2Me)Lpz)(NCX)2] (L = N,N'-dimethyl-N,N'-bis((pyrazin-2-yl)methyl)-1,2-ethanediamine and X = S (1), BH3 (2)), have been synthesized and characterized by single-crystal X-ray diffraction, magnetic, optical reflectivity, and photomagnetic measurements. They have similar FeN6 coordination environments offered by the tetradentate ligand with a cis-α conformation and two NCX(-) coligands in cis positions. However, 1 and 2 have different molecular arrangements and crystal packings, and are isolated in orthorhombic Pbnb and monoclinic C2/c space groups, respectively...
September 22, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28937648/separation-and-enrichment-of-lectin-from-zihua-snap-bean-phaseolus-vulgaris-seeds-by-peg-600-ammonium-sulfate-aqueous-two-phase-system
#6
Bin Jiang, Yongqiang Yuan, Xiaoqing Zhang, Zhibiao Feng, Chunhong Liu
A fast and efficient method based on a polyethylene glycol (PEG) 600/(NH₄)₂SO₄ aqueous two-phase system for extracting lectin from Zihua snap-bean (Phaseolus vulgaris) seeds was established. According to a Box-Behnken design (BBD), involving four factors at three levels each subjected to analysis of variance (ANOVA) and response surface analysis, the protein recovery and the purification factor of lectin in the top phase were used as the response values of the variance analysis to acquire the multivariate quadratic regression model...
September 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28937605/production-of-poly-%C3%AE%C2%B5-caprolactone-hydroxyapatite-composite-scaffolds-with-a-tailored-macro-micro-porous-structure-high-mechanical-properties-and-excellent-bioactivity
#7
Jong-Woo Kim, Kwan-Ha Shin, Young-Hag Koh, Min Jin Hah, Jiyoung Moon, Hyoun-Ee Kim
We produced poro-us poly(ε-caprolactone) (PCL)/hydroxyapatite (HA) composite scaffolds for bone regeneration, which can have a tailored macro/micro-porous structure with high mechanical properties and excellent in vitro bioactivity using non-solvent-induced phase separation (NIPS)-based 3D plotting. This innovative 3D plotting technique can create highly microporous PCL/HA composite filaments by inducing unique phase separation in PCL/HA solutions through the non-solvent-solvent exchange phenomenon. The PCL/HA composite scaffolds produced with various HA contents (0 wt %, 10 wt %, 15 wt %, and 20 wt %) showed that PCL/HA composite struts with highly microporous structures were well constructed in a controlled periodic pattern...
September 22, 2017: Materials
https://www.readbyqxmd.com/read/28936848/-simultaneous-determination-of-seven-alkaloids-and-three-flavonoids-in-sophorae-tonkinensis-radix-et-rhizoma-by-hplc
#8
Fu-Man Han, Li-Xin Wang, Ying Chen, Liang-Mian Chen, Wei-Hong Feng, Jin-Yu Wang, De-Wen Liu, Yun You, Yan Tong
In this study, an HPLC method was developed for simultaneous determination of seven alkaloids (cytosine, oxymatrine, N-oxysophocarpine, N-methylcytisine, sophoranol, matrine, and sophocarpine) and three flavonoids (trifolirhizin, fermononetin, and maackiain) from different samples of Sophorae Tonkinensis Radix et Rhizoma. Samples were analyzed on a Welch XtimateTM C₁₈ column (4. 6 mm× 250 mm, 5 μm) eluted with the mobile phase of acetonitrile (A) and 0.01 mol•L⁻¹ ammonium acetate solution (pH 8.0) (B) in a linear gradient mode as follows: 0-20 min,4%-14% A;20-30 min,14%-25% A;30-45 min,25%-40% A;45-65 min,40%-55% A;65-75 min,55% A...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28936842/-rapid-analysis-on-chemical-constituents-in-yinyanghuo-zonghuangtong-capsule-by-uplc-q-tof-ms-ms
#9
Xue-E Yu, Jian-Ping Qin, Jia-Chun Li, Wen-Zhe Huang, Zhen-Zhong Wang, Wei Xiao
To analyze and identify the chemical constituents from Yinyanghuo Zonghuangtong capsule by using UPLC/Q-TOF-MS/MS. Chromatographic separation was carried out on an Agilent SB-C₁₈ column (2.1 mm×100 mm, 1.8 μm) at the temperature of 30 ℃. The mobile phase was 0.1% formic acid and acetonitrile by gradient elution, with a flow rate at 0.30 mL•min⁻¹, and the injection volume of 2 μL. The MS spectrum was acquired in both negative and positive ion modes using ESI ion source. Based on relative accurate molecular weight, secondary mass spectrometry pyrolysis fragments and chromatographic peak retention time, as well as fragmentation regularity summarized from reference substance and the literature, we could effectively identify the chemical structure of the components under test...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28935776/liquid-phase-condensation-in-cell-physiology-and-disease
#10
REVIEW
Yongdae Shin, Clifford P Brangwynne
Phase transitions are ubiquitous in nonliving matter, and recent discoveries have shown that they also play a key role within living cells. Intracellular liquid-liquid phase separation is thought to drive the formation of condensed liquid-like droplets of protein, RNA, and other biomolecules, which form in the absence of a delimiting membrane. Recent studies have elucidated many aspects of the molecular interactions underlying the formation of these remarkable and ubiquitous droplets and the way in which such interactions dictate their material properties, composition, and phase behavior...
September 22, 2017: Science
https://www.readbyqxmd.com/read/28934961/area-asymmetry-of-heart-rate-variability-signal
#11
Chang Yan, Peng Li, Lizhen Ji, Lianke Yao, Chandan Karmakar, Changchun Liu
BACKGROUND: Heart rate fluctuates beat-by-beat asymmetrically which is known as heart rate asymmetry (HRA). It is challenging to assess HRA robustly based on short-term heartbeat interval series. METHOD: An area index (AI) was developed that combines the distance and phase angle information of points in the Poincaré plot. To test its performance, the AI was used to classify subjects with: (i) arrhythmia, and (ii) congestive heart failure, from the corresponding healthy controls...
September 21, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28934611/enhanced-biodegradation-of-atrazine-by-bacteria-encapsulated-in-organically-modified-silica-gels
#12
Joey J Benson, Jonathan K Sakkos, Adi Radian, Lawrence P Wackett, Alptekin Aksan
Biodegradation by cells encapsulated in silica gel is an economical and environmentally friendly method for the removal of toxic chemicals from the environment. In this work, recombinant E. coli expressing atrazine chlorohydrolase (AtzA) were encapsulated in organically modified silica (ORMOSIL) gels composed of TEOS, silica nanoparticles (SNPs), and either phenyltriethoxysilane (PTES) or methyltriethoxysilane (MTES). ORMOSIL gels adsorbed much higher amounts of atrazine than the hydrophilic TEOS gels. The highest amount of atrazine adsorbed by ORMOSIL gels was 48...
September 11, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28933706/the-influence-of-atmosphere-on-performance-of-pure-phase-wz-and-zb-inas-nanowire-transistors
#13
Abu Rifat Ullah, Hannah J Joyce, Hoe Tan, Chennupati Jagadish, Adam P Micolich
We compare the characteristics of phase-pure MOCVD grown ZB and WZ InAs nanowire transistors in several atmospheres: air, dry pure N<sub>2</sub> and O<sub>2</sub>, and N<sub>2</sub> bubbled through liquid H<sub>2</sub>O and alcohols to identify whether phase-related structural/surface differences affect their response. Both WZ and ZB give poor gate characteristics in dry state. Adsorption of polar species reduces off-current by 2-3 orders of magnitude, increases on-off ratio and significantly reduces sub-threshold slope...
September 21, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28933483/selective-separation-of-furfural-and-hydroxymethylfurfural-from-an-aqueous-solution-using-a-supported-hydrophobic-deep-eutectic-solvent-liquid-membrane
#14
Carin H J T Dietz, Maaike C Kroon, Michela Di Stefano, Martin van Sint Annaland, Fausto Gallucci
For the first time, 12 different supported deep eutectic solvent (DES) liquid membranes were prepared and characterized. These membranes consist of a polymeric support impregnated with a hydrophobic DES. First, the different membranes were characterized and their stability in water and air was determined. Subsequently, the supported DES liquid membranes were applied for the recovery of furfural (FF) and hydroxymethylfurfural (HMF) from aqueous solutions. The effects of substrate properties (e.g. pore size), DES properties (e...
September 21, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28933014/size-exclusion-chromatography-ion-mobility-mass-spectrometry-coupling-a-step-toward-structural-biology
#15
Guillaume Van der Rest, Frédéric Halgand
Noncovalent interactions are essential for the structural organization of biomacromolecules in cells. For this reason, the study of the biophysical, dynamic, and architectural interactions among biomacromolecules is essential. Since mass spectrometry requires compatible solutions while preserving the noncovalent bonding network, we envisioned that size exclusion chromatography coupled with ion mobility and mass spectrometry would be a valuable technique to desalt the initial sample and provide solution and gas-phase structural information in a single stage experiment...
September 20, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28932828/acceleration-of-diffusion-in-ethylammonium-nitrate-ionic-liquid-confined-between-parallel-glass-plates
#16
Andrei Filippov, Oleg I Gnezdilov, Nicklas Hjalmarsson, Oleg N Antzutkin, Sergei Glavatskih, István Furó, Mark W Rutland
Diffusion of EAN confined between polar glass plates separated by a few micrometers is higher by a factor of ca. 2 as compared to bulk values. Formation of a new phase, different to the bulk, was suggested.
September 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28932270/repeat-treatment-with-rifaximin-improves-irritable-bowel-syndrome-related-quality-of-life-a-secondary-analysis-of-a-randomized-double-blind-placebo-controlled-trial
#17
Brooks D Cash, Mark Pimentel, Satish S C Rao, Leonard Weinstock, Lin Chang, Zeev Heimanson, Anthony Lembo
BACKGROUND: Diarrhea-predominant irritable bowel syndrome (IBS-D) impairs patient quality of life (QOL). Rifaximin is an oral, nonsystemic antibiotic indicated for IBS-D. The objective of this secondary analysis was to evaluate rifaximin retreatment on IBS-related QOL in patients with IBS-D. METHODS: Patients received open-label rifaximin 550 mg three times daily for 2 weeks. Clinical responders [simultaneously meeting weekly response criteria for abdominal pain (⩾30% improvement from baseline in mean weekly pain score) and stool consistency (⩾50% decrease from baseline in number of days/week with Bristol Stool Scale (BSS) type 6 or 7 stools) during ⩾2 of first 4 weeks posttreatment] who relapsed during an up to 18-week treatment-free observation phase were randomly assigned to receive two 2-week courses of double-blind rifaximin or placebo, separated by 10 weeks...
September 2017: Therapeutic Advances in Gastroenterology
https://www.readbyqxmd.com/read/28932116/silk-fibroin-cartilage-extracellular-matrix-scaffolds-with-sequential-delivery-of-tgf-%C3%AE-3-for-chondrogenic-differentiation-of-adipose-derived-stem-cells
#18
Qiang Yang, Bin-Hong Teng, Li-Na Wang, Kun Li, Chen Xu, Xin-Long Ma, Yang Zhang, De-Ling Kong, Lian-Yong Wang, Yan-Hong Zhao
A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28931852/improvement-of-a-fermentation-process-for-the-production-of-two-pfama1-dico-based-malaria-vaccine-candidates-in-pichia-pastoris
#19
Robin Kastilan, Alexander Boes, Holger Spiegel, Nadja Voepel, Ivana Chudobová, Stephan Hellwig, Johannes Felix Buyel, Andreas Reimann, Rainer Fischer
Pichia pastoris is a simple and powerful expression platform that has the ability to produce a wide variety of recombinant proteins, ranging from simple peptides to complex membrane proteins. A well-established fermentation strategy is available comprising three main phases: a batch phase, followed by a glycerol fed-batch phase that increases cell density, and finally an induction phase for product expression using methanol as the inducer. We previously used this three-phase strategy at the 15-L scale to express three different AMA1-DiCo-based malaria vaccine candidates to develop a vaccine cocktail...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931476/greenhouse-gas-and-ammonia-emissions-from-production-of-compost-bedding-on-a-dairy-farm
#20
M A Fillingham, A C VanderZaag, S Burtt, H Baldé, N M Ngwabie, W Smith, A Hakami, C Wagner-Riddle, S Bittman, D MacDonald
Recent developments in composting technology enable dairy farms to produce their own bedding from composted manure. This management practice alters the fate of carbon and nitrogen; however, there is little data available documenting how gaseous emissions are impacted. This study measured in-situ emissions of methane (CH4), carbon dioxide (CO2), nitrous oxide (N2O), and ammonia (NH3) from an on-farm solid-liquid separation system followed by continuously-turned plug-flow composting over three seasons. Emissions were measured separately from the continuously-turned compost phase, and the compost-storage phase prior to the compost being used for cattle bedding...
September 18, 2017: Waste Management
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