Use the keywords feature with a free QxMD account.
Use Read by QxMD to access full text via your institution or open access sources.
Read also provides personalized recommendations to keep you up to date in your field.
Existing User
Sign InNew to Read
Sign Up#21
JOURNAL ARTICLE
Xinping Huang, Yan Zhao, Min Wei, Ruipeng Zhuge, Xiaofeng Zheng
Cellular senescence is a major process affected by multiple signals and coordinated by a complex signal response network. Identification of novel regulators of cellular senescence and elucidation of their molecular mechanisms will aid the discovery of new treatment strategies for aging-related diseases. In the present study, we identified human coilin-interacting nuclear ATPase protein (hCINAP) as a negative regulator of aging. Depletion of cCINAP significantly shortened the lifespan of Caenorhabditis elegans and accelerated primary cell aging...
March 6, 2023: Journal of Molecular Cell Biology
#22
Qin Feng, Hong Kim, Amrita Barua, Luping Huang, Modupeola Bolaji, Sharon Zachariah, Sung Yun Jung, Bin He, Tianyi Zhou, Aroshi Mitra
Prostate cancer is the most commonly diagnosed noncutaneous cancer in American men. TDRD1, a germ cell-specific gene, is erroneously expressed in more than half of prostate tumors, but its role in prostate cancer development remains elusive. In this study, we identified a PRMT5-TDRD1 signaling axis that regulates the proliferation of prostate cancer cells. PRMT5 is a protein arginine methyltransferase essential for small nuclear ribonucleoprotein (snRNP) biogenesis. Methylation of Sm proteins by PRMT5 is a critical initiation step for assembling snRNPs in the cytoplasm, and the final snRNP assembly takes place in Cajal bodies in the nucleus...
February 22, 2023: Research Square
#23
JOURNAL ARTICLE
Edward Courchaine, Sara Gelles-Watnick, Martin Machyna, Korinna Straube, Sarah Sauyet, Jade Enright, Karla M Neugebauer
Cajal bodies (CBs) are ubiquitous nuclear membraneless organelles (MLOs) that concentrate and promote efficient biogenesis of snRNA-protein complexes involved in splicing (snRNPs). Depletion of the CB scaffolding protein coilin disperses snRNPs, making CBs a model system for studying the structure and function of MLOs. Although it is assumed that CBs form through condensation, the biomolecular interactions responsible remain elusive. Here, we discover the unexpected capacity of coilin's N-terminal domain (NTD) to form extensive fibrils in the cytoplasm and discrete nuclear puncta in vivo...
October 12, 2022: Nature Communications
#24
JOURNAL ARTICLE
Miryam Pérez-Cañamás, Michael Taliansky, Carmen Hernández
To establish productive infections, viruses must be able both to subdue the host metabolism for their own benefit and to counteract host defences. This frequently results in the establishment of viral-host protein-protein interactions that may have either proviral or antiviral functions. The study of such interactions is essential for understanding the virus-host interplay. Plant viruses with RNA genomes are typically translated, replicated, and encapsidated in the cytoplasm of infected cells. Despite this, a significant array of their encoded proteins has been reported to enter the nucleus, often showing high accumulation at subnuclear structures such as the nucleolus and/or Cajal bodies...
July 22, 2022: Plants (Basel, Switzerland)
#25
JOURNAL ARTICLE
Anders Bergström, David W G Stanton, Ulrike H Taron, Laurent Frantz, Mikkel-Holger S Sinding, Erik Ersmark, Saskia Pfrengle, Molly Cassatt-Johnstone, Ophélie Lebrasseur, Linus Girdland-Flink, Daniel M Fernandes, Morgane Ollivier, Leo Speidel, Shyam Gopalakrishnan, Michael V Westbury, Jazmin Ramos-Madrigal, Tatiana R Feuerborn, Ella Reiter, Joscha Gretzinger, Susanne C Münzel, Pooja Swali, Nicholas J Conard, Christian Carøe, James Haile, Anna Linderholm, Semyon Androsov, Ian Barnes, Chris Baumann, Norbert Benecke, Hervé Bocherens, Selina Brace, Ruth F Carden, Dorothée G Drucker, Sergey Fedorov, Mihály Gasparik, Mietje Germonpré, Semyon Grigoriev, Pam Groves, Stefan T Hertwig, Varvara V Ivanova, Luc Janssens, Richard P Jennings, Aleksei K Kasparov, Irina V Kirillova, Islam Kurmaniyazov, Yaroslav V Kuzmin, Pavel A Kosintsev, Martina Lázničková-Galetová, Charlotte Leduc, Pavel Nikolskiy, Marc Nussbaumer, Cóilín O'Drisceoil, Ludovic Orlando, Alan Outram, Elena Y Pavlova, Angela R Perri, Małgorzata Pilot, Vladimir V Pitulko, Valerii V Plotnikov, Albert V Protopopov, André Rehazek, Mikhail Sablin, Andaine Seguin-Orlando, Jan Storå, Christian Verjux, Victor F Zaibert, Grant Zazula, Philippe Crombé, Anders J Hansen, Eske Willerslev, Jennifer A Leonard, Anders Götherström, Ron Pinhasi, Verena J Schuenemann, Michael Hofreiter, M Thomas P Gilbert, Beth Shapiro, Greger Larson, Johannes Krause, Love Dalén, Pontus Skoglund
The grey wolf (Canis lupus) was the first species to give rise to a domestic population, and they remained widespread throughout the last Ice Age when many other large mammal species went extinct. Little is known, however, about the history and possible extinction of past wolf populations or when and where the wolf progenitors of the present-day dog lineage (Canis familiaris) lived1-8 . Here we analysed 72 ancient wolf genomes spanning the last 100,000 years from Europe, Siberia and North America. We found that wolf populations were highly connected throughout the Late Pleistocene, with levels of differentiation an order of magnitude lower than they are today...
July 2022: Nature
#26
JOURNAL ARTICLE
Madelyn K Logan, Katheryn E Lett, Douglas M McLaurin, Michael D Hebert
The nuclear factor-Kappa B (NF-κB) pathway is a crucial mediator of inflammatory signaling. Aberrant activation of NF-κB is associated with several disorders including preeclampsia (PE). Many regulators of the NF-κB pathway have been identified, including microRNAs (miRNAs). Specifically, miR-517-3p targets mRNA encoding TNFAIP3 Interacting Protein 1 (TNIP1), an inhibitor of NF-κB signaling. Activation of NF-κB increases production of the cytokine TNF superfamily member 15 (TNFSF15), leading to the upregulation of anti-angiogenic soluble vascular endothelial growth factor receptor 1 (sFlt-1)...
July 15, 2022: Biology Open
#27
JOURNAL ARTICLE
Aala A Abulfaraj, Hanna M Alhoraibi, Kiruthiga Mariappan, Jean Bigeard, Huoming Zhang, Marilia Almeida-Trapp, Olga Artyukh, Fatimah Abdulhakim, Sabiha Parween, Delphine Pflieger, Ikram Blilou, Heribert Hirt, Naganand Rayapuram
Biogenesis of ribonucleoproteins occurs in dynamic subnuclear compartments called Cajal bodies (CBs). COILIN is a critical scaffolding component essential for CB formation, composition, and activity. We recently showed that Arabidopsis (Arabidopsis thaliana) AtCOILIN is phosphorylated in response to bacterial elicitor treatment. Here, we further investigated the role of AtCOILIN in plant innate immunity. Atcoilin mutants are compromised in defense responses to bacterial pathogens. Besides confirming a role of AtCOILIN in alternative splicing (AS), Atcoilin showed differential expression of genes that are distinct from those of AS, including factors involved in RNA biogenesis, metabolism, plant immunity, and phytohormones...
August 29, 2022: Plant Physiology
#28
JOURNAL ARTICLE
McKinzie M Frederick, Elissa N Mai, David P Hurley, Laura L Schoeneman, Emily F Ruff
Vaccinia-related kinase 1 (VRK1) is a serine/threonine kinase that plays a variety of roles in transcription regulation and the cell cycle. Its substrates include histones, p53, and coilin. The kinase activity of the VRK1 protein is largely controlled by its C-terminal tail, which interacts with the enzyme active site. Several rare point mutations in VRK1 (including L195V, R89Q, and Y213H) are associated with neurodegenerative disorders, and questions remain as to how these mutations affect VRK1's intrinsic stability and activity...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
#29
JOURNAL ARTICLE
Madelyn K Logan, Douglas McLaurin, Katheryn Lett, Michael Hebert
Cajal bodies (CBs) are membraneless subnuclear domains that contribute to the biogenesis and maturation of ribonucleoproteins (RNPs) which take part in fundamental cellular activities such as translation, pre-mRNA splicing, and telomere maintenance. Partitioning of CBs occurs through liquid-liquid phase separation (LLPS) where the CB marker protein, coilin, is thought to scaffold the CB. However, the formation of CBs and the regulation of CB number has not been fully elucidated. Disease states such as cancer and preeclampsia have altered CB expression, thus additional insight into CB condensation would provide an important cellular model system that could be used to investigate these and other diseases...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
#30
JOURNAL ARTICLE
Katheryn Lett, Madelyn Logan, Douglas McLaurin, Michael Hebert
Coilin was first identified as the marker protein for Cajal Bodies (CBs). However, CBs are only abundant in neuronal, embryonic, and cancer cells, while primary cells have few CBs. Even in cells with CBs, 70% of coilin is nucleoplasmic, not found in CBs. CBs are subnuclear domains that function in small nuclear ribonucleoprotein (snRNP) biogenesis as well as production of telomerase RNP, expression and 3'end processing of small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), and small CB-associated RNAs (scaRNAs)...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
#31
JOURNAL ARTICLE
Douglas McLaurin, Madelyn Davis, Katheryn Lett, Michael Hebert
Cajal bodies (CBs) are nuclear bodies that are hosts for the modification and assembly of ribonucleoproteins (RNPs). The nuclear protein coilin is known as the CB marker protein as it is the scaffold for CB assembly and vital for structural integrity. However, there is still not much understood about the function of coilin. Recent studies by our lab implicate coilin in the biogenesis of microRNAs (miRNAs). Currently, there are sparse options in animal models for studying coilin and CB function that account for limitations such as off-target effects of coilin depletion or incomplete protein knockdown...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
#32
JOURNAL ARTICLE
Davide A Basello, A Gregory Matera, David Staněk
Coilin is a conserved protein essential for integrity of nuclear membrane-less inclusions called Cajal bodies. Here, we report an amino acid substitution (p.K496E) found in a widely-used human EGFP-coilin construct that has a dominant-negative effect on Cajal body formation. We show that this coilin-K496E variant fails to rescue Cajal bodies in cells lacking endogenous coilin, whereas the wild-type construct restores Cajal bodies in mouse and human coilin-knockout cells. In cells containing endogenous coilin, both the wild-type and K496E variant proteins accumulate in Cajal bodies...
April 15, 2022: Journal of Cell Science
#33
JOURNAL ARTICLE
Bojie Xu, Ruicheng Yang, Bo Yang, Liang Li, Jiaqi Chen, Jiyang Fu, Xinyi Qu, Dong Huo, Chen Tan, Huanchun Chen, Zhong Peng, Xiangru Wang
Escherichia coli is the most common gram-negative pathogenic bacterium causing meningitis. It penetrates the blood-brain barrier (BBB) and activates nuclear factor kappa B (NF-κB) signaling, which are vital events leading to the development of meningitis. Long non-coding RNAs (lncRNAs) have been implicated in regulating neuroinflammatory signaling, and our previous study showed that E. coli can induce differential expression of lncRNAs, including lncC11orf54-1, in human brain microvascular endothelial cells (hBMECs)...
January 3, 2022: Molecular Brain
#34
JOURNAL ARTICLE
Patricia Morejon-Garcia, Boris Keren, Iñigo Marcos-Alcalde, Paulino Gomez-Puertas, Fanny Mochel, Pedro A Lazo
Background and Objectives: To conduct a genetic and molecular functional study of a family with members affected of hereditary spastic paraplegia (HSP) of unknown origin and carrying a novel pathogenic vaccinia-related kinase 1 ( VRK 1) variant. Methods: Whole-exome sequencing was performed in 2 patients, and their parents diagnosed with HSP. The novel VRK 1 variant was detected by whole-exome sequencing, molecularly modeled and biochemically characterized in kinase assays...
October 2021: Neurology. Genetics
#35
JOURNAL ARTICLE
Eimear Cleary, Martin Boudou, Patricia Garvey, Coilin Oh Aiseadha, Paul McKeown, Jean O'Dwyer, Paul Hynds
The Republic of Ireland regularly reports the highest annual crude incidence rates of Shiga toxin-producing Escherichia coli (STEC) enteritis in the European Union, ≈10 times the average. We investigated spatiotemporal patterns of STEC enteritis in Ireland using multiple statistical tools. Overall, we georeferenced 2,755 cases of infection during January 2013-December 2017; we found >1 case notified in 2,340 (12.6%) of 18,641 Census Small Areas. We encountered the highest case numbers in children 0-5 years of age (n = 1,101, 39...
September 2021: Emerging Infectious Diseases
#36
JOURNAL ARTICLE
Martin Boudou, Coilin ÓhAiseadha, Patricia Garvey, Jean O'Dwyer, Paul Hynds
BACKGROUND: To constrain propagation and mitigate the burden of COVID-19, most countries initiated and continue to implement several non-pharmaceutical interventions (NPIs), including national and regional lockdowns. In the Republic of Ireland, the first national lockdown was decreed on 23rd of March 2020, followed by a succession of restriction increases and decreases (phases) over the following year. To date, the effects of these interventions remain unclear, and particularly within differing population subsets...
2021: PloS One
#37
JOURNAL ARTICLE
Katheryn E Lett, Madelyn K Logan, Douglas M McLaurin, Michael D Hebert
MicroRNAs (miRNAs) are ∼22 nt small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The biogenesis of miRNAs involves a series of processing steps beginning with cropping of the primary miRNA transcript by the Microprocessor complex, which is comprised of Drosha and DGCR8. Here we report a novel regulatory interaction between the Microprocessor components and coilin, the Cajal Body (CB) marker protein...
July 28, 2021: Molecular Biology of the Cell
#38
REVIEW
Ruidan Xu, Yongfeng Yang, Xiaofeng Zheng
hCINAP (human coilin-interacting nuclear ATPase protein), also known as AK6 (adenylate kinase 6), is an atypical adenylate kinase with critical roles in many biological processes including gene transcription, ribosome synthesis, cell metabolism, cell proliferation and apoptosis, DNA damage responses, and genome stability. Also, hCINAP/AK6 dysfunction is associated with cancer and various inflammatory diseases. In this review, we summarize the structural features and biological roles of hCINAP in several important signaling pathways, as well as its connection with tumor onset and progression...
July 10, 2021: FEBS Letters
#39
JOURNAL ARTICLE
Kathryn R DeLeo, Sonu S Baral, Alex Houser, Allison James, Phelan Sewell, Shova Pandey, Patrick J DiMario
Nucleolar stress occurs when ribosome production or function declines. Nucleolar stress in stem cells or progenitor cells often leads to disease states called ribosomopathies. Drosophila offers a robust system to explore how nucleolar stress causes cell cycle arrest, apoptosis, or autophagy depending on the cell type. We provide an overview of nucleolar stress in Drosophila by depleting nucleolar phosphoprotein of 140 kDa (Nopp140), a ribosome biogenesis factor (RBF) in nucleoli and Cajal bodies (CBs). The depletion of Nopp140 in eye imaginal disc cells generates eye deformities reminiscent of craniofacial deformities associated with the Treacher Collins syndrome (TCS), a human ribosomopathy...
June 23, 2021: International Journal of Molecular Sciences
#40
JOURNAL ARTICLE
Madelyn K Logan, Katheryn E Lett, Michael D Hebert
Hypoxia is a severe stressor to cellular homeostasis. At the cellular level, low oxygen triggers the transcription of a variety of genes supporting cell survival and oxygen homeostasis mediated by transcription factors, such as hypoxia-inducible factors (HIFs). Among many determinants dictating cell responses to hypoxia and HIFs are microRNAs (miRNAs). Cajal bodies (CBs), subnuclear structures involved in ribonucleoprotein biogenesis, have been recently proven to contribute to miRNA processing and biogenesis but have not been studied under hypoxia...
June 15, 2021: Journal of Cell Science
Make the most of Read.
Download the mobile app.
Download the mobile app.
Fetching more papers... 
