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https://www.readbyqxmd.com/read/28092827/conjugated-polymer-with-carboxylate-groups-hg-2-system-as-a-turn-on-fluorescence-probe-for-label-free-detection-of-cysteine-containing-compounds
#1
Hongyu Mi, Mingming Guan, Jilin Liu, Hongyan Shan, Qiang Fei, Yanfu Huan, Guodong Feng
In this work, a turn on fluorescent sensor, based on Hg(2+) coordination conjugated polymer, was developed to detect cysteine-containing compounds. The fluorescence of conjugated polymer (poly(2,5-bis (sodium 4-oxybutyrate) -1,4 - phenylethynylene-alt-1,4-phenyleneethynylene; PPE-OBS) would be quenched by Hg(2+) because of the coordination-induced aggregation and electron transfers of PPE-OBS toward Hg(2+). When there were some cysteine-containing compounds in PPE-OBS-Hg(2+) system, the fluorescence of PPE-OBS would be recovered...
January 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28091574/an-inert-pesticide-adjuvant-synergizes-viral-pathogenicity-and-mortality-in-honey-bee-larvae
#2
Julia D Fine, Diana L Cox-Foster, Christopher A Mullin
Honey bees are highly valued for their pollination services in agricultural settings, and recent declines in managed populations have caused concern. Colony losses following a major pollination event in the United States, almond pollination, have been characterized by brood mortality with specific symptoms, followed by eventual colony loss weeks later. In this study, we demonstrate that these symptoms can be produced by chronically exposing brood to both an organosilicone surfactant adjuvant (OSS) commonly used on many agricultural crops including wine grapes, tree nuts and tree fruits and exogenous viral pathogens by simulating a horizontal transmission event...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28089395/royal-jelly-ameliorates-diet-induced-obesity-and-glucose-intolerance-by-promoting-brown-adipose-tissue-thermogenesis-in-mice
#3
Takeshi Yoneshiro, Ryuji Kaede, Kazuki Nagaya, Julia Aoyama, Mana Saito, Yuko Okamatsu-Ogura, Kazuhiro Kimura, Akira Terao
INTRODUCTION: Identification of thermogenic food ingredients is potentially a useful strategy for the prevention of obesity and related metabolic disorders. It has been reported that royal jelly (RJ) supplementation improves insulin sensitivity; however, its impacts on energy expenditure and adiposity remain elusive. We investigated anti-obesity effects of RJ supplementation and their relation to physical activity levels and thermogenic capacities of brown (BAT) and white adipose tissue (WAT)...
January 11, 2017: Obesity Research & Clinical Practice
https://www.readbyqxmd.com/read/28088343/first-confirmed-report-of-a-bacterial-brood-disease-in-stingless-bees
#4
Jenny Lee Shanks, Anthony Mark Haigh, Markus Riegler, Robert Neil Spooner-Hart
Susceptibility to brood pathogens in eusocial stingless bees (Meliponini), alternative pollinators to honey bees, is unknown. Brood losses in managed colonies of the Australian stingless bee, Tetragonula carbonaria, were studied over 20 months. We isolated a disease-causing bacterium, Lysinibacillus sphaericus (Firmicutes, Bacillaceae), from worker and queen larvae, brood cell provisions and honey stores. Pathogenicity experiments confirmed this bacterium as the causal organism. It took 22 days from infection to first appearance of brood disease symptoms...
January 11, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28081188/effect-of-varroa-destructor-wounding-and-varroa-homogenate-on-gene-expression-in-brood-and-adult-honey-bees
#5
Gun Koleoglu, Paul H Goodwin, Mariana Reyes-Quintana, Mollah Md Hamiduzzaman, Ernesto Guzman-Novoa
Honey bee (Apis mellifera) gene expression related to immunity for hymenoptaecin (AmHym) and defensin-1 (AmDef-1), longevity for vitellogenin (AmVit2) and stem cell proliferation for poly U binding factor 68 kDa (AmPuf68) was compared following Varroa destructor parasitism, buffer injection and injection of V. destructor compounds in its homogenate. In adults, V. destructor parasitism decreased expression of all four genes, while buffer injection decreased expression of AmHym, AmPuf68 and AmVit2, and homogenate injection decreased expression of AmPuf68 and AmVit2 but increased expression of AmDef-1 relative to their respective controls...
2017: PloS One
https://www.readbyqxmd.com/read/28077022/feasibility-of-a-novel-multispot-nanoarray-for-antibiotic-screening-in-honey
#6
Sara E McNamee, Giulia Rosar, Lidija Persic, Christopher T Elliott, Katrina Campbell
Practical solutions for multiple antibiotic determination in food is required by the food industry and regulators for cost effective screening purposes. This study describes the feasibility in development and preliminary performance of a novel multispot nanoarray for antibiotic screening in honey. Using a multiplex approach the metabolites of the four main nitrofuran antibiotics including morpholinomethyl-2-oxazolidone (AMOZ), 3-amino-2-oxazolidinone (AOZ), semicarbazide (SEM), 1-aminohydantoin (AHD) and chloramphenicol (CAP) were simultaneously detected...
January 12, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28074124/involvement-of-blood-mononuclear-cells-in-the-infertility-age-associated-diseases-and-cancer-treatment
#7
REVIEW
Antonin Bukovsky
Blood mononuclear cells consist of T cells and monocyte derived cells. Beside immunity, the blood mononuclear cells belong to the complex tissue control system (TCS), where they exhibit morphostatic function by stimulating proliferation of tissue stem cells followed by cellular differentiation, that is stopped after attaining the proper functional stage, which differs among various tissue types. Therefore, the term immune and morphostatic system (IMS) should be implied. The TCS-mediated morphostasis also consists of vascular pericytes controlled by autonomic innervation, which is regulating the quantity of distinct tissues in vivo...
December 26, 2016: World Journal of Stem Cells
https://www.readbyqxmd.com/read/28073057/neonicotinoids-transference-from-the-field-to-the-hive-by-honey-bees-towards-a-pesticide-residues-biomonitor
#8
Niell Silvina, Jesús Florencia, Pérez Nicolás, Pérez Cecilia, Pareja Lucía, Silvana Abbate, Carrasco-Letelier Leonidas, Díaz Sebastián, Mendoza Yamandú, Cesio Verónica, Heinzen Horacio
The beehive as a quantitative monitor of pesticide residues applied over a soybean crop was studied through a semi field experiment of controlled exposure of honey bees to pesticides in macro tunnels. The distribution within exposed beehives of pesticides commonly used in soybean plantation, was assessed. Residue levels of insecticides in soybean leaves, honey bees, wax, honey and pollen were analyzed. The transference from pesticides present in the environment into the beehive was evidenced. The obtained results allow relating pesticide concentrations present in the environment with traces found in foraging bees...
January 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28069631/pollination-reservoirs-in-lowbush-blueberry-ericales-ericaceae
#9
E M Venturini, F A Drummond, A K Hoshide, A C Dibble, L B Stack
Pollinator-dependent agriculture heavily relies upon a single pollinator-the honey bee. To diversify pollination strategies, growers are turning to alternatives. Densely planted reservoirs of pollen- and nectar-rich flowers (pollination reservoirs, hereafter "PRs") may improve pollination services provided by wild bees. Our focal agroecosystem, lowbush blueberry (Vaccinium angustifolium Aiton), exists in a simple landscape uniquely positioned to benefit from PRs. First, we contrast bee visitation rates and use of three types of PR...
January 9, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28068984/effect-of-tualang-honey-against-ka-induced-oxidative-stress-and-neurodegeneration-in-the-cortex-of-rats
#10
Nur Shafika Mohd Sairazi, Sirajudeen K N S, Mohd Asnizam Asari, Swamy Mummedy, Mustapha Muzaimi, Siti Amrah Sulaiman
BACKGROUND: Administration of KA on rodents has resulted in seizures, behavioral changes, oxidative stress, and neuronal degeneration on selective population of neurons in the brain. The present study was undertaken to investigate the extent of neuroprotective effect conferred by Malaysian Tualang Honey (TH), an antioxidant agent, in the cerebral cortex of rats against KA-induced oxidative stress and neurodegeneration in an animal model of KA-induced excitotoxicity. METHODS: Male Sprague-Dawley rats were randomly divided into five groups: Control, KA-treated group, TH + KA-treated group, aspirin (ASP; anti-inflammatory agent) + KA-treated group and topiramate (TPM; antiepileptic agent) + KA-treated group...
January 9, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28063405/effect-of-the-egyptian-propolis-on-the-hepatic-antioxidant-defense-and-pro-apoptotic-p53-and-anti-apoptotic-bcl2-expressions-in-aflatoxin-b1-treated-male-mice
#11
Abeer A Alm-Eldeen, Mohammed A Basyony, Nabil K Elfiky, Mohamed M Ghalwash
Aflatoxins are potent hepatotoxic due to their role in producing reactive oxygen species and consequently peroxidative damage. Propolis is a honey bee product known for its antioxidant capacity. The aim of this study was to verify the antioxidant effect of the Egyptian propolis extract (EPE) against aflatoxin B1 (AFB1)-induced hepatotoxicity in mice. Forty eight male mice were divided: first, second and third groups were used as control receiving saline, olive oil and EPE respectively, fourth was AFB1 group, fifth and sixth received EPE post or pre AFB1 treatment, respectively...
January 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28063093/honey-and-wound-healing-an-update
#12
REVIEW
Sami K Saikaly, Amor Khachemoune
For centuries, honey has been utilized for wound healing purposes. In recent times, this specific topic has become a field of interest, possibly due to the advent of antibiotic resistance in microbial pathogens. With constant technological advancement, the information regarding honey's mechanisms of action on wound healing has accumulated at a rapid pace. Similarly, clinical studies comparing honey with traditional wound care therapies are steadily emerging. As a follow-up to a previous review published in the journal in 2011, the current review article outlines publications regarding honey and wound healing that have been published between June 2010 and August 2016...
January 6, 2017: American Journal of Clinical Dermatology
https://www.readbyqxmd.com/read/28062536/a-comparison-of-honey-bee-collected-pollen-from-working-agricultural-lands-using-light-microscopy-and-its-metabarcoding
#13
M D Smart, R S Cornman, D D Iwanowicz, M McDermott-Kubeczko, J S Pettis, M S Spivak, C R V Otto
Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been tested on a spatially or temporally robust number of pollen samples. Here, we compare pollen identification results derived from light microscopy and DNA sequencing techniques with samples collected from honey bee colonies embedded within a gradient of intensive agricultural landscapes in the Northern Great Plains throughout the 2010-2011 growing seasons...
January 6, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28062458/hyperosmotic-agents-and-antibiotics-affect-dissolved-oxygen-and-ph-concentration-gradients-in-staphylococcus-aureus-biofilms
#14
Mia Mae Kiamco, Erhan Atci, Abdelrhman Mohamed, Douglas R Call, Haluk Beyenal
: Biofilms on wound surfaces are treated topically with hyperosmotic agents such as medical-grade honey and cadexomer iodine; in some cases, these treatments are combined with antibiotics. Tissue repair requires oxygen, and a low pH is conducive to oxygen release from red blood cells and epithelialization. We investigated the variation of dissolved oxygen concentration and pH with biofilm depth, and the variation in oxygen consumption rates when biofilms are challenged with medical-grade honey or cadexomer iodine combined with vancomycin or ciprofloxacin...
January 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28062435/flaccid-paralysis-in-an-infant-associated-with-a-dirty-wound-and-application-of-honey
#15
Charlotte Jane Joseph, Teik Beng Khoo, Keng Yee Lee
An infant, who was born preterm at 36 weeks, presented with fever and ulcer at umbilical region which progressed to necrotising fasciitis of anterior abdominal wall. He was treated with intravenous penicillin, intravenous cloxacillin and local application of medicated honey. Subsequently, he required wound debridement. Postoperatively, he required prolonged invasive ventilation due to poor respiratory effort which was associated with hypotonia and areflexia. Nerve conduction study revealed absent responses...
January 6, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28060305/dissection-and-observation-of-honey-bee-dorsal-vessel-for-studies-of-cardiac-function
#16
Scott T O'Neal, Troy D Anderson
The European honey bee, Apis mellifera L., is a valuable agricultural and commercial resource noted for producing honey and providing crop pollination services, as well as an important model social insect used to study memory and learning, aging, and more. Here we describe a detailed protocol for the dissection of the dorsal abdominal wall of a bee in order to visualize its dorsal vessel, which serves the role of the heart in the insect. A successful dissection will expose a functional heart that, under the proper conditions, can maintain a steady heartbeat for an extended period of time...
December 12, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28054920/evaluation-of-honey-as-a-topical-therapy-for-intraoral-wound-healing-in-rats
#17
Goli Chamani, Mohammad Reza Zarei, Mitra Mehrabani, Ali Mehdavinezhad, Mehrangiz Vahabian, Fatemah Ahmadi-Motamayel
OBJECTIVE: Honey is one of the oldest known medicines. Its medical and therapeutic importance has been recently rediscovered. Honey is an effective treatment for infected wounds and ulcers. The aim of this study was to determine the effect of honey as a topical therapy for intraoral wound healing in rats. MATERIALS AND METHODS: Thirty-two male rats were divided into experimental and control groups (consisting of 16 rats, 4 animals in each group). A 2-mm mucosal defect was made to the depth of the periosteum using punch biopsy...
December 29, 2016: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/28053589/nectar-secretion-dynamics-and-honey-production-potentials-of-some-major-honey-plants-in-saudi-arabia
#18
Nuru Adgaba, Ahmed Al-Ghamdi, Yilma Tadesse, Awraris Getachew, Awad M Awad, Mohammad J Ansari, Ayman A Owayss, Seif Eldin A Mohammed, Abdulaziz S Alqarni
The contribution of a bee plant species to honey production depends on the plant's nectar secretion quality and quantity, which is mainly governed by biotic and abiotic factors. The aim of the current study, was to investigate the nectar secretion dynamics and honey production potential of 14 major bee plant species of the target area. We examined the quantity and dynamics of nectar sugar per flower five times a day using a nectar sugar washing technique and direct measuring of nectar with calibrated capillary tubes...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28053207/dynamics-of-weight-change-and-temperature-of-apis-mellifera-hymenoptera-apidae-colonies-in-a-wintering-building-with-controlled-temperature
#19
E Stalidzans, A Zacepins, A Kviesis, V Brusbardis, J Meitalovs, L Paura, N Bulipopa, M Liepniece
Honey bee wintering in a wintering building (indoors) with controlled microclimate is used in some cold regions to minimize colony losses due to the hard weather conditions. The behavior and possible state of bee colonies in a dark room, isolated from natural environment during winter season, was studied by indirect temperature measurements to analyze the expression of their annual rhythm when it is not affected by ambient temperature, rain, snow, wind, and daylight. Thus, the observed behavior in the wintering building is initiated solely by bee colony internal processes...
January 4, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28052206/does-addition-of-propolis-to-glass-ionomer-cement-alter-its-physicomechanical-properties-an-in-vitro-study
#20
P Subramaniam, K L Girish Babu, G Neeraja, S Pillai
: Propolis is a natural resinous substance produced by honey bees. The antimicrobial effects of glass ionomer cement have been shown to improve with the addition of propolis; however its effect on the physicomechanical properties of the cement is not known. AIM: The purpose of this study was to evaluate the compressive strength and solubility of conventional restorative glass ionomer cement following the addition of propolis. STUDY DESIGN: Twenty half cylindrical samples were prepared with conventional restorative glass ionomer cement formed the control group...
2017: Journal of Clinical Pediatric Dentistry
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