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https://www.readbyqxmd.com/read/28213961/chrysin-induces-death-of-prostate-cancer-cells-by-inducing-ros-and-er-stress
#1
Soomin Ryu, Whasun Lim, Fuller W Bazer, Gwonhwa Song
Chrysin is a natural flavone found in numerous plant extracts, honey and propolis that has multiple biological activities including anti-cancer effects. Understanding of biological mechanisms mediated in response to chrysin in cancerous cells may provide novel insight into chemotherapeutic approaches with reduced side effects in cancers. In the present study, we investigated functional roles of chrysin in progression of prostate cancer cells using DU145 and PC-3 cell lines. The results showed that chrysin induced apoptosis of cells evidenced by DNA fragmentation and increasing the population of both DU145 and PC-3 cells in the sub-G1 phase of the cell cycle...
February 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28213209/molecularly-imprinted-solid-phase-extraction-method-for-simultaneous-determination-of-seven-nitroimidazoles-from-honey-by-hplc-ms-ms
#2
Xiu-Chun Guo, Zhao-Yang Xia, Hai-Hui Wang, Wen-Yi Kang, Li-Ming Lin, Wen-Qing Cao, Hong-Wei Zhang, Wen-Hui Zhou
In this work, a selective sample cleanup procedure that combined molecular imprinting technique with solid phase extraction was developed for the simultaneous extraction of the seven nitroimidazoles (NMZs) from honey samples. The molecular imprinting polymers for NMZs were prepared through bulk polymerization method using 2-methyl-5-nitroimidazole as template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking agent. The obtained molecular imprinting polymers showed high affinity to template molecule and was used as selective sorbent for simultaneously selective extraction of the seven NMZs from honey matrix...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28212637/a-pectin-honey-hydrogel-prevents-postoperative-intraperitoneal-adhesions-in-a-rat-model
#3
Gessica Giusto, Cristina Vercelli, Selina Iussich, Andrea Audisio, Emanuela Morello, Rosangela Odore, Marco Gandini
BACKGROUND: Adhesions are a common postoperative surgical complication. Liquid honey has been used intraperitoneally to reduce the incidence of these adhesions. However, solid barriers are considered more effective than liquids in decreasing postoperative intra-abdominal adhesion formation; therefore, a new pectin-honey hydrogel (PHH) was produced and its effectiveness was evaluated in a rat cecal abrasion model. Standardized cecal/peritoneal abrasion was performed through laparotomy in 48 adult Sprague-Dawley rats to induce peritoneal adhesion formation...
February 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28209053/non-targeted-screening-and-determination-of-sulfonamides-a-dispersive-micro-solid-phase-extraction-approach-to-the-analysis-of-milk-and-honey-samples-using-liquid-chromatography-high-resolution-mass-spectrometry
#4
Shuping Hu, Min Zhao, Yiyuan Xi, Qiqi Mao, Xudong Zhou, Dawei Chen, Pengcheng Yan
A simple, rapid, sensitive, selective, and environmentally friendly method, based on dispersive micro solid-phase extraction approach (dispersive micro SPE) coupled with liquid chromatography-high resolution mass spectrometry (LC-HRMS) was established for the analysis of sulfonamides in honey and milk. An efficient non-targeted screening strategy was designed to discover and identify known and unknown sulfonamides in honey and milk using full-MS/all ion fragmentation (AIF) mass spectrometry acquisition mode...
February 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28208761/cold-ambient-temperature-promotes-nosema-spp-intensity-in-honey-bees-apis-mellifera
#5
Gina Retschnig, Geoffrey R Williams, Annette Schneeberger, Peter Neumann
Interactions between parasites and environmental factors have been implicated in the loss of managed Western honey bee (=HB, Apis mellifera) colonies. Although laboratory data suggest that cold temperature may limit the spread of Nosema ceranae, an invasive species and now ubiquitous endoparasite of Western HBs, the impact of weather conditions on the distribution of this microsporidian in the field is poorly understood. Here, we conducted a survey for Nosema spp. using 18 Swiss apiaries (four colonies per apiary) over a period of up to 18 months...
February 9, 2017: Insects
https://www.readbyqxmd.com/read/28207237/simultaneous-determination-of-absolute-configuration-and-quantity-of-lipopeptides-using-chiral-liquid-chromatography-mass-spectrometry-and-diastereomeric-internal-standards
#6
Reza Nemati, Christopher Dietz, Emily Anstadt, Robert Clark, Michael B Smith, Frank Nichols, Xudong Yao
Lipopeptides promote innate immune response and are related to disease pathology. To investigate the newly-emerging roles of lipopeptides, accurate measurements of stereoisomers with multiple chiral centers are essential yet challenging. This work uses (3R)- and (3S)-(15-methyl-3-((13-methyltetradecanoyl)oxy)hexadecanoyl)glycyl-L-serine, abbreviated as L-serine-(R+S)-Lipid 654, to develop a method that combines chiral liquid chromatography, a diastereomeric mixture of isotopically-labeled internal standards and multiple reaction monitoring mass spectrometry...
February 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28207182/immune-system-stimulation-by-the-gut-symbiont-frischella-perrara-in-the-honey-bee-apis-mellifera
#7
Olivier Emery, Konstantin Schmidt, Philipp Engel
Gut bacteria engage in various symbiotic interactions with their host and impact gut immunity and homeostasis in different ways. In honey bees, the gut microbiota is composed of a relatively simple, but highly specialized bacterial community. One of its members, the gammaproteobacterium Frischella perrara induces the so-called scab phenotype, a dark-colored band that develops on the epithelial surface of the pylorus. To understand the underlying host response, we analyzed transcriptome changes in the gut in response to bacterial colonization...
February 16, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28205632/using-dna-metabarcoding-to-investigate-honey-bee-foraging-reveals-limited-flower-use-despite-high-floral-availability
#8
Natasha de Vere, Laura E Jones, Tegan Gilmore, Jake Moscrop, Abigail Lowe, Dan Smith, Matthew J Hegarty, Simon Creer, Col R Ford
Understanding which flowers honey bees (Apis mellifera) use for forage can help us to provide suitable plants for healthy honey bee colonies. Accordingly, honey DNA metabarcoding provides a valuable tool for investigating pollen and nectar collection. We investigated early season (April and May) floral choice by honey bees provided with a very high diversity of flowering plants within the National Botanic Garden of Wales. There was a close correspondence between the phenology of flowering and the detection of plants within the honey...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28199005/specialized-photoreceptor-composition-in-the-raptor-fovea
#9
Mindaugas Mitkus, Peter Olsson, Matthew B Toomey, Joseph C Corbo, Almut Kelber
The retinae of many bird species contain a depression with high photoreceptor density known as the fovea. Many species of raptors have two foveae, a deep central fovea and a shallower temporal fovea. Birds have six types of photoreceptors: rods, active in dim light, double cones that are thought to mediate achromatic discrimination, and four types of single cones mediating color vision. To maximize visual acuity, the fovea should only contain photoreceptors contributing to high-resolution vision. Interestingly, it has been suggested that raptors might lack double cones in the fovea...
February 15, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28193870/disruption-of-quercetin-metabolism-by-fungicide-affects-energy-production-in-honey-bees-apis-mellifera
#10
Wenfu Mao, Mary A Schuler, May R Berenbaum
Cytochrome P450 monooxygenases (P450) in the honey bee, Apis mellifera, detoxify phytochemicals in honey and pollen. The flavonol quercetin is found ubiquitously and abundantly in pollen and frequently at lower concentrations in honey. Worker jelly consumed during the first 3 d of larval development typically contains flavonols at very low levels, however. RNA-Seq analysis of gene expression in neonates reared for three days on diets with and without quercetin revealed that, in addition to up-regulating multiple detoxifying P450 genes, quercetin is a negative transcriptional regulator of mitochondrion-related nuclear genes and genes encoding subunits of complexes I, III, IV, and V in the oxidative phosphorylation pathway...
February 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28193412/lepteridine-as-a-unique-fluorescent-marker-for-the-authentication-of-manuka-honey
#11
Bin Lin, Kerry M Loomes, Gordana Prijic, Ralf Schlothauer, Jonathan M Stephens
The recent discovery of two unique manuka marker fluorescence wavelengths (MM1 and MM2) potentially offers a rapid and cost-effective approach for manuka honey authentication using spectroscopy. The fluorophore responsible for the MM1 marker has been identified as leptosperin. We investigated whether lepteridine may be responsible for the MM2 fluorescence. We quantified the lepteridine in manuka honey and manuka nectar, which ranged between 5-52mg/kg and 80-205mg/kg, respectively. Notably, the fluorescent spectrum of synthetic lepteridine matched the MM2 fluorescence signature...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28192487/volatile-organic-compounds-of-thai-honeys-produced-from-several-floral-sources-by-different-honey-bee-species
#12
Praetinee Pattamayutanon, Sergio Angeli, Prodpran Thakeow, John Abraham, Terd Disayathanoowat, Panuwan Chantawannakul
The volatile organic compounds (VOCs) of four monofloral and one multifloral of Thai honeys produced by Apis cerana, Apis dorsata and Apis mellifera were analyzed by headspace solid-phase microextraction (HS-SPME) followed by gas chromatography and mass spectrometry (GC-MS). The floral sources were longan, sunflower, coffee, wild flowers (wild) and lychee. Honey originating from longan had more VOCs than all other floral sources. Sunflower honey had the least numbers of VOCs. cis-Linalool oxide, trans-linalool oxide, ho-trienol, and furan-2,5-dicarbaldehyde were present in all the honeys studied, independent of their floral origin...
2017: PloS One
https://www.readbyqxmd.com/read/28192130/ielvis-an-open-source-matlab-toolbox-for-localizing-and-visualizing-human-intracranial-electrode-data
#13
David M Groppe, Stephan Bickel, Andrew R Dykstra, Xiuyuan Wang, Pierre Mégevand, Manuel R Mercier, Fred A Lado, Ashesh D Mehta, Christopher J Honey
BACKGROUND: Intracranial electrical recordings (iEEG) and brain stimulation (iEBS) are invaluable human neuroscience methodologies. However, the value of such data is often unrealized as many laboratories lack tools for localizing electrodes relative to anatomy. To remedy this, we have developed a MATLAB toolbox for intracranial electrode localization and visualization, iELVis. NEW METHOD: iELVis uses existing tools (BioImage Suite, FSL, and FreeSurfer) for preimplant magnetic resonance imaging (MRI) segmentation, neuroimaging coregistration, and manual identification of electrodes in postimplant neuroimaging...
February 9, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28191986/studies-of-learned-helplessness-in-honey-bees-apis-mellifera-ligustica
#14
Christopher W Dinges, Christopher A Varnon, Lisa D Cota, Stephen Slykerman, Charles I Abramson
The current study reports 2 experiments investigating learned helplessness in the honey bee (Apis mellifera ligustica). In Experiment 1, we used a traditional escape method but found the bees' activity levels too high to observe changes due to treatment conditions. The bees were not able to learn in this traditional escape procedure; thus, such procedures may be inappropriate to study learned helplessness in honey bees. In Experiment 2, we used an alternative punishment, or passive avoidance, method to investigate learned helplessness...
February 13, 2017: Journal of Experimental Psychology. Animal Learning and Cognition
https://www.readbyqxmd.com/read/28191328/combination-of-nigella-sativa-and-honey-in-eradication-of-gastric-helicobacter-pylori-infection
#15
Fataneh Hashem-Dabaghian, Shahram Agah, Maryam Taghavi-Shirazi, Ali Ghobadi
BACKGROUND: Gastric Helicobacter pylori is extremely common worldwide. OBJECTIVES: The aim of this study was to assess the effectiveness of combination of Nigella sativa and honey (Dosin) in eradication of gastric H. pylori infection. PATIENTS AND METHODS: Nineteen patients who had positive result for H. pylori infection by urea breath test (UBT) without a past history of peptic ulcer, gastric cancer or gastrointestinal bleeding, were suggested to receive one teaspoon of the mixture of Dosin (6 g/day of N...
November 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28187732/comparative-studies-on-the-multi-component-pharmacokinetics-of-aristolochiae-fructus-and-honey-fried-aristolochiae-fructus-extracts-after-oral-administration-in-rats
#16
Jinbin Yuan, Gang Ren, Jian Liang, Chong-Zhi Wang, Zhihong Yan, Qun Huang, Jiankang Li, Yang Chen, Yi Tang, Xiaofei Liu, Chun-Su Yuan
BACKGROUND: Aristolochiae Fructus (AF) and honey-fried Aristolochiae Fructus (HAF) have been used in China for a long time as anti-tussive and expectorant drugs. Few clinical cases have been reported to be associated with the toxicity of AF and HAF, although relatively high amounts of aristolochic acids (AAs) have been found in them. Our previous experiments have verified from the chemical changes and from traditional toxicology that honey-processing can significantly reduce the toxicity of AF...
February 10, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28187378/development-of-an-analytical-method-for-detecting-nitrofurans-in-bee-pollen-by-liquid-chromatography-electrospray-ionization-tandem-mass-spectrometry
#17
Mi Sun Park, Kyung Tae Kim, Jong Seong Kang
Bee pollen collected by honeybees, which is in powdered form, is a good nutritional supplement. Nitrofuran antibiotics are assumed not to be present in bee pollen, which is important as the level of antibiotics in bee pollen is strongly regulated in many countries. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect nitrofurans in honey has been developed, but this method is not suitable for bee pollen because of it being in powdered form. During preparation of bee pollen samples, the dispersal of powder particles in an aqueous solution often makes them susceptible to forming an emulsion with solvent components such as hexane and ethyl acetate...
January 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28185119/biallelic-brca2-mutations-in-two-black-south-african-children-with-fanconi-anaemia
#18
Candice Feben, Careni Spencer, Anneline Lochan, Nakita Laing, Karen Fieggen, Engela Honey, Tasha Wainstein, Amanda Krause
Fanconi anaemia (FA) is a genotypically and phenotypically heterogeneous genetic condition, characterized cytogenetically by chromosomal instability and breakage secondary to impaired DNA repair mechanisms. Affected individuals typically manifest growth restriction and congenital physical abnormalities and most progress to hematological disease including bone marrow aplasia. A rare genetic subtype of FA (FA-D1) is caused by biallelic mutations in the BRCA2 gene. Affected individuals manifest severe congenital anomalies and significant pigmentary changes and are additionally at risk for early onset leukemia and certain solid organ malignancies, including Wilms tumors and brain tumors...
February 9, 2017: Familial Cancer
https://www.readbyqxmd.com/read/28183884/celebrating-wellbeing-in-practice
#19
(no author information available yet)
The winners of the inaugural Wellbeing Awards, an initiative run by the Society of Practising Veterinary Surgeons (SPVS) and the Mind Matters Initiative, were announced at the joint congress of the SPVS and the Veterinary Practice Management Association last month. During the meeting, representatives of the winning practices joined a discussion of how practices can help ensure they have happy and fulfilled teams. Laura Honey reports.
February 11, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28183287/price-elasticity-of-the-demand-for-soft-drinks-other-sugar-sweetened-beverages-and-energy-dense-food-in-chile
#20
Carlos M Guerrero-López, Mishel Unar-Munguía, M Arantxa Colchero
BACKGROUND: Chile is the second world's largest per capita consumer of caloric beverages. Caloric beverages are associated with overweight, obesity and other chronic diseases. The objective of this study is to estimate the price elasticity of demand for soft drinks, other sugar-sweetened beverages and high-energy dense foods in urban areas in Chile in order to evaluate the potential response of households' consumption to changes in prices. METHODS: We used microdata from the VII Family Budget Survey 2012-2013, which collects information on expenditures made by Chilean urban households on items such as beverages and foods...
February 10, 2017: BMC Public Health
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