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https://www.readbyqxmd.com/read/28430801/honey-bees-preferentially-consume-freshly-stored-pollen
#1
Mark J Carroll, Nicholas Brown, Craig Goodall, Alexandra M Downs, Timothy H Sheenan, Kirk E Anderson
Honey bees (Apis mellifera) collect and store both honey and pollen in preserved forms. Pollen storage involves the addition of honey or nectar and oral secretions to pollen granules. It is controversial whether the duration of pollen storage alters the palatability or nutritive value of the pollen storage medium. We examined how bees utilize different-aged stored pollen during an extended pollen flow. The deposition of pollen into wax cells and subsequent consumption were monitored daily on 18 brood frames from 6 colonies over an 8d observation period...
2017: PloS One
https://www.readbyqxmd.com/read/28429131/the-first-comprehensive-molecular-detection-of-six-honey-bee-viruses-in-iran-in-2015-2016
#2
Mohammadreza Ghorani, Omid Madadgar, Arash Ghalyanchi Langeroudi, Mohammadreza Rezapanah, Sedigheh Nabian, Hesameddin Akbarein, Reza Kh Farahani, Hossein Maghsoudloo, Hamed Abdollahi, Mohammad Forsi
At least 18 viruses have been reported in the honey bee (Apis mellifera L.). However, severe diseases in honey bees are mainly caused by six viruses, and these are the most important in beekeeping. These viruses include: deformed wing virus (DWV), acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), sacbrood virus (SBV), kashmir bee virus (KBV), and black queen cell virus (BQCV). In this study, we evaluated 89 Iranian honey bee apiaries (during the period 2015-2016) suffering from symptoms of depopulation, sudden collapse, paralysis, or dark coloring, by employing reverse transcription-PCR...
April 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28426165/hydrogen-peroxide-dependent-antibacterial-action-of-melilotus-albus-honey
#3
Patrycja Sowa, Dorota Grabek-Lejko, Monika Wesołowska, Sylwia Swacha, Małgorzata Dżugan
Honey originating from different floral sources exhibits the broad-spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as non peroxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time. Polish melilot honey samples (Melilotus albus biennal -3 and annual-5, Melilotus officinalis -1) were collected directly from beekeepers and analyzed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolics contents as well as antibacterial properties against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp...
April 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28424101/limited-impacts-of-truck-based-ultra-low-volume-applications-of-mosquito-adulticides-on-mortality-in-honey-bees-apis-mellifera
#4
F D Rinkevich, J W Margotta, V Pokhrel, T W Walker, R H Vaeth, W C Hoffman, B K Fritz, R G Danka, T E Rinderer, R L Aldridge, K J Linthicum, J A Ottea, K B Healy
Adulticides applied against mosquitoes can reduce vector populations during times of high arbovirus transmission. However, impacts of these insecticides on pollinators and other non-target organisms are of concern to mosquito control professionals, beekeepers and others. We evaluated mortality of Culex quinquefasciatus and Apis mellifera when caged insects were exposed to low and high label rates of four common adulticides (Aqua-Pursuit™ [permethrin], Duet® [prallethrin + sumithrin], Fyfanon® [malathion] and Scourge® [resmethrin]) at six distances up to 91...
April 20, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28422139/high-pesticide-risk-to-honey-bees-despite-low-focal-crop-pollen-collection-during-pollination-of-a-mass-blooming-crop
#5
Scott H McArt, Ashley A Fersch, Nelson J Milano, Lauren L Truitt, Katalin Böröczky
Honey bees provide critical pollination services for many agricultural crops. While the contribution of pesticides to current hive loss rates is debated, remarkably little is known regarding the magnitude of risk to bees and mechanisms of exposure during pollination. Here, we show that pesticide risk in recently accumulated beebread was above regulatory agency levels of concern for acute or chronic exposure at 5 and 22 of the 30 apple orchards, respectively, where we placed 120 experimental hives. Landscape context strongly predicted focal crop pollen foraging and total pesticide residues, which were dominated by fungicides...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28420790/honeybee-gut-microbiota-promotes-host-weight-gain-via-bacterial-metabolism-and-hormonal-signaling
#6
Hao Zheng, J Elijah Powell, Margaret I Steele, Carsten Dietrich, Nancy A Moran
Social bees harbor a simple and specialized microbiota that is spatially organized into different gut compartments. Recent results on the potential involvement of bee gut communities in pathogen protection and nutritional function have drawn attention to the impact of the microbiota on bee health. However, the contributions of gut microbiota to host physiology have yet to be investigated. Here we show that the gut microbiota promotes weight gain of both whole body and the gut in individual honey bees. This effect is likely mediated by changes in host vitellogenin, insulin signaling, and gustatory response...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28417271/antimicrobial-effects-of-manuka-honey-on-in-vitro-biofilm-formation-by-clostridium-difficile
#7
M Piotrowski, P Karpiński, H Pituch, A van Belkum, P Obuch-Woszczatyński
Clostridium difficile is the cause of the nosocomial C. difficile infection (CDI). The conventional antibiotics used in CDI therapy are often unsuccessful, and recurrent infections may occur. Biofilm formation by C. difficile is associated with chronic or recurrent infections; biofilms may contribute to virulence and impaired antimicrobial efficacy. Manuka honey, derived from the Manuka tree (Leptospermum scoparium), is known to exhibit antimicrobial properties that are associated with its significant content of methylglyoxal, a natural antibiotic...
April 18, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28417225/comparative-proteomic-analysis-provides-insight-into-10-hydroxy-2-decenoic-acid-biosynthesis-in-honey-bee-workers
#8
Xiao-Hui Yang, Shi-Fa Yang, Rui-Ming Wang
10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis was performed in the MGs of workers with different 10-HDA production. In total, 59 up-regulated protein species representing 45 unique proteins were identified in high 10-HDA-producing workers by 2-DE-MALDI-TOF/TOF MS...
April 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28417086/blood-immune-function-parameters-in-response-to-combined-aerobic-dance-exercise-and-honey-supplementation-in-adult-women
#9
Marhasiyah Rahim, Foong Kiew Ooi, Wan Zuraida Wan Abdul Hamid
To date, information on the effectiveness of combined aerobic dance exercise with honey supplementation on immune function in women is lacking. The present study investigated the effects of 8 weeks of combined aerobic dance exercise and honey supplementation on blood immune function parameters in adult women. In this study, forty four healthy sedentary women (25-40 year-old) were assigned into four groups with n = 11 per group: sedentary without supplementation control (Con), honey supplementation (H), aerobic dance exercise (D) and combined aerobic dance exercise with honey supplementation (HD) groups...
April 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28415841/unique-pattern-of-protein-bound-maillard-reaction-products-in-manuka-leptospermum-scoparium-honey
#10
Michael Hellwig, Jana Rückriemen, Daniel Sandner, Thomas Henle
Honeys from the New Zealand Manuka tree (Leptospermum scoparium) contain substantial amounts of dihydroxyacetone (DHA) and methylglyoxal (MGO). We hypothesized that the abundance of MGO should result in a particular pattern of protein-bound Maillard reaction products (MRPs) in Manuka honey. A protein-rich high-molecular weight fraction was isolated from 12 Manuka and 8 non-Manuka honeys and hydrolyzed enzymatically. By HPLC-MS/MS, 8 MRPs, namely N-ε-fructosyllysine, N-ε-maltulosyllysine, carboxymethyllysine, carboxyethyllysine (CEL), pyrraline, formyline, maltosine, and methylglyoxal-derived hydroimidazolone 1 (MG-H1), were quantitated...
April 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28412203/atp-sensitive-inwardly-rectifying-potassium-channel-modulators-alter-cardiac-function-in-honey-bees
#11
Scott T O'Neal, Daniel R Swale, Jeffrey R Bloomquist, Troy D Anderson
ATP-sensitive inwardly rectifying potassium (KATP) channels couple cellular metabolism to the membrane potential of the cell and play an important role in a variety of tissue types, including the insect dorsal vessel, making them a subject of interest not only for understanding invertebrate physiology, but also as a potential target for novel insecticides. Most of what is known about these ion channels is the result of work performed in mammalian systems, with insect studies being limited to only a few species and physiological systems...
April 13, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28411017/biological-properties-of-propolis-extracts-something-new-from-an-ancient-product
#12
REVIEW
Nada Zabaiou, Allan Fouache, Amalia Trousson, Silvère Baron, Amar Zellagui, Mesbah Lahouel, Jean-Marc A Lobaccaro
Natural products are an interesting source of new therapeutics, especially for cancer therapy as 70% of them have botany origin. Propolis, a resinous mixture that honey bees collect and transform from tree buds, sap flows, or other botanical sources, has been used by ethnobotany and traditional practitioners as early in Egypt as 3000 BC. Enriched in flavonoids, phenol acids and terpene derivatives, propolis has been widely used for its antibacterial, antifungal and anti-inflammatory properties. Even though it is a challenge to standardize propolis composition, chemical analyses have pointed out interesting molecules that also present anti-oxidant and anti-proliferative properties that are of interest in the field of anti-cancer therapy...
April 11, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28409237/prevalence-of-sucretolerant-bacteria-in-common-soils-and-their-isolation-and-characterization
#13
Casper Fredsgaard, Donald B Moore, Fei Chen, Benton C Clark, Mark A Schneegurt
Sucretolerant microbes grow in the presence of sugar concentrations high enough to substantially lower water activities. Natural habitats high in sugars are mainly limited to dried fruit, floral nectar, honey, sugarcane, and associated soils. Organisms that tolerate extremes of solute concentration, high enough to lower water activities, might not be expected in common oligoosmotic soils. We report on the isolation of sucretolerant bacteria from common soils using media supplemented with 50% sucrose (a w 0...
April 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28407954/total-determination-of-chloramphenicol-residues-in-foods-by-liquid-chromatography-tandem-mass-spectrometry
#14
Hiroyuki Kikuchi, Takatoshi Sakai, Reiko Teshima, Satoru Nemoto, Hiroshi Akiyama
A simple and sensitive analytical method for the determination of chloramphenicol (CAP) and chloramphenicol glucuronide (CAPG) residues in foods such as livestock products, seafood, honey and royal jelly was developed. The method comprises solvent extraction with methanol, enzymatic hydrolysis with β-glucuronidase and clean-up using a hydrophilic lipophilic balanced copolymer solid phase extraction column. To determine the optimal conditions for the complete enzymatic hydrolysis of CAPG, we examined the effect of enzyme concentration and incubation time on the hydrolysis...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407946/manuka-honey-leptospermum-scoparium-inhibits-jack-bean-urease-activity-due-to-methylglyoxal-and-dihydroxyacetone
#15
Jana Rückriemen, Oliver Klemm, Thomas Henle
Manuka honey (Leptospermum scoparium) exerts a strong antibacterial effect. Bacterial enzymes are an important target for antibacterial compounds. The enzyme urease produces ammonia and enables bacteria to adapt to an acidic environment. A new enzymatic assay, based on photometric detection of ammonia with ninhydrin, was developed to study urease activity. Methylglyoxal (MGO) and its precursor dihydroxyacetone (DHA), which are naturally present in manuka honey, were identified as jack bean urease inhibitors with IC50 values of 2...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28406359/enantioselective-analysis-of-chloramphenicol-residues-in-honey-samples-by-chiral-lc-msms-and-results-of-a-honey-survey
#16
Gerhard G Rimkus, Dirk Hoffmann
Chloramphenicol (CAP) is a broad-spectrum antibiotic used widely both in human and in veterinary medication. Since 1994 CAP is not authorised anymore for use in food-producing animals in the EU due to several adverse effects. A minimum required performance level (MRPL) 0.3 µg kg(-1) was established in 2003. CAP molecule contains two asymmetric centres thus in total four para-CAP stereoisomers exist. Only the RR-CAP enantiomer is bioactive having significant antimicrobial activity. For the first time a chiral LC/MSMS method is reported to identify and quantify the four CAP enantiomers at residue levels in honey samples...
April 13, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28406050/investigation-of-pyrrolizidine-alkaloids-including-their-respective-n-oxides-in-selected-food-products-available-in-hong-kong-by-liquid-chromatography-electrospray-ionization-mass-spectrometry
#17
Stephen W C Chung, Aaron C H Lam
The aim of this study was to determine the levels of pyrrolizidine alkaloids (Shimshoni, Mulder et al.), including their respective N-oxides, in foodstuffs available in Hong Kong, by liquid chromatography-electrospray ionization tandem mass spectrometry. A total of 234 samples (48 food items) were collected randomly from local market and analyzed. About 50% of samples were found to contain detectable amount of PAs. Amongst the 48 food items, PAs were not detected in 11 food items, including barley flour, beef, cattle liver, pork, pig liver, chicken meat, chicken liver, milk, non-fermented tea, Melissa tea and linden tea...
April 13, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28406019/honey-extracted-polyphenolics-reduce-experimental-hypoxia-in-human-keratinocytes-culture
#18
Amrita Chaudhary, Swarnendu Bag, Provas Banerjee, Jyotirmoy Chatterjee
Hypoxic assault affects fundamental cellular processes and generates oxidative stress on healthy cells/molecules. Honey extracted polyphenolics (HEP) as a natural antioxidant reduced hypoxic cytotoxicity in this study. Different honey samples were physicochemically characterized to identify preferred (jamun) honey [pH 3.55 ± 0.04, conductivity (μs/cm) = 6.66 ± 0.14, water content % (w/w) = 14.70 ± 0.35, total solid content % (w/w) = 85.30 ± 0.35, phenol content (mg GAE/100 g) = 403.55 ± 0.35, flavonoid content (mg QE/100 g) = 276...
April 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28404729/wings-as-impellers-honey-bees-co-opt-flight-system-to-induce-nest-ventilation-and-disperse-pheromones
#19
Jacob M Peters, Nick Gravish, Stacey A Combes
Honey bees (Apis mellifera) are remarkable fliers that regularly carry heavy loads of nectar and pollen, supported by a flight system - the wings, thorax and flight muscles - that one might assume is optimized for aerial locomotion. However, honey bees also use this system to perform other crucial tasks that are unrelated to flight. When ventilating the nest, bees grip the surface of the comb or nest entrance and fan their wings to drive airflow through the nest, and a similar wing-fanning behavior is used to disperse volatile pheromones from the Nasonov gland...
April 12, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28403242/brain-metabolomic-profiling-of-eastern-honey-bee-apis-cerana-infested-with-the-mite-varroa-destructor
#20
Jiang-Li Wu, Chun-Xue Zhou, Peng-Jie Wu, Jin Xu, Yue-Qin Guo, Fei Xue, Awraris Getachew, Shu-Fa Xu
The mite Varroa destructor is currently the greatest threat to apiculture as it is causing a global decrease in honey bee colonies. However, it rarely causes serious damage to its native hosts, the eastern honey bees Apis cerana. To better understand the mechanism of resistance of A. cerana against the V. destructor mite, we profiled the metabolic changes that occur in the honey bee brain during V. destructor infestation. Brain samples were collected from infested and control honey bees and then measured using an untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based global metabolomics method, in which 7918 and 7462 ions in ESI+ and ESI- mode, respectively, were successfully identified...
2017: PloS One
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