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https://www.readbyqxmd.com/read/28813014/antibacterial-properties-of-nonwoven-wound-dressings-coated-with-manuka-honey-or-methylglyoxal
#1
Sophie E L Bulman, Giuseppe Tronci, Parikshit Goswami, Chris Carr, Stephen J Russell
Manuka honey (MH) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. MH provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (MGO). Aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both MH and MGO (at equivalent MGO concentrations) when applied as a physical coating to a nonwoven fabric wound dressing. When physically coated on to a cellulosic hydroentangled nonwoven fabric, it was found that concentrations of 0...
August 16, 2017: Materials
https://www.readbyqxmd.com/read/28812768/chemical-nutritive-composition-and-a-wide-range-of-bioactive-properties-of-honey-mushroom-armillaria-mellea-vahl-fr-kummer
#2
Marina Kostić, Marija Smiljković, Jovana Petrović, Jasmina Glamočlija, Lillian Barros, Isabel C F R Ferreira, Ana Ćirić, Marina Soković
A. mellea fruiting bodies collected from nature were chemically characterized and shown to be rich in carbohydrates (81.25 g per 100 g dw), ash, fat and proteins (8.84 g per 100 g dw, 1.97 g per 100 g dw and 1.81 g per 100 g dw, respectively). Mannitol was the main free sugar while malic acid was the most abundant organic acid. δ-Tocopherol was the dominant form of tocopherols with 42.41 μg per 100 g dw. Polyunsaturated fatty acids were predominant, followed by saturated and monounsaturated fatty acids. A methanolic extract prepared from these samples was tested for antioxidant, quorum sensing and antimicrobial assays, as well as for its cytotoxicity effects...
August 16, 2017: Food & Function
https://www.readbyqxmd.com/read/28812560/an-invasive-social-insect-overcomes-genetic-load-at-the-sex-locus
#3
Rosalyn Gloag, Guiling Ding, Joshua R Christie, Gabriele Buchmann, Madeleine Beekman, Benjamin P Oldroyd
Some invasive hymenopteran social insects found new populations with very few reproductive individuals. This is despite the high cost of founder effects for such insects, which generally require heterozygosity at a single locus-the complementary sex determiner, csd-to develop as females. Individuals that are homozygous at csd develop as either infertile or subfertile diploid males or not at all. Furthermore, diploid males replace the female workers that are essential for colony function. Here we document how the Asian honey bee (Apis cerana) overcame the diploid male problem during its invasion of Australia...
November 7, 2016: Nature ecology & evolution
https://www.readbyqxmd.com/read/28812253/the-land-of-no-milk-and-no-honey-force-feeding-in-israel
#4
Zohar Lederman, Shmuel Lederman
In 2015, the Israeli Knesset passed the force-feeding act that permits the director of the Israeli prison authority to appeal to the district court with a request to force-feed a prisoner against his expressed will. A recent position paper by top Israeli clinicians and bioethicists, published in Hebrew, advocates for force-feeding by medical professionals and presents several arguments that this would be appropriate. Here, we first posit three interrelated questions: 1. Do prisoners have a right to hunger-strike? 2...
August 15, 2017: Monash Bioethics Review
https://www.readbyqxmd.com/read/28809782/dietary-supplementation-of-honey-bee-larvae-with-arginine-and-abscisic-acid-enhances-nitric-oxide-and-granulocyte-immune-responses-after-trauma
#5
Pedro Negri, Leonor Ramirez, Silvina Quintana, Nicolás Szawarski, Matías Maggi, Yves Le Conte, Lorenzo Lamattina, Martin Eguaras
Many biotic and abiotic stressors impact bees' health, acting as immunosupressors and contribute to colony losses. Thus, the importance of studying the immune response of honey bees is central to develop new strategies aiming to enhance bees' fitness to confront the threats affecting them. If a pathogen breaches the physical and chemical barriers, honey bees can protect themselves from infection with cellular and humoral immune responses which represent a second line of defense. Through a series of correlative studies we have previously reported that abscisic acid (ABA) and nitric oxide (NO) share roles in the same immune defenses of Apis mellifera (A...
August 15, 2017: Insects
https://www.readbyqxmd.com/read/28808376/isolation-of-abscisic-acid-from-korean-acacia-honey-with-anti-helicobacter-pylori-activity
#6
SeGun Kim, InPyo Hong, SoonOk Woo, HyeRi Jang, SokCheon Pak, SangMi Han
BACKGROUND: Helicobacter pylori (H. pylori) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide. OBJECTIVE: This study is aimed at evaluating the anti-H. pylori activity of Korean acacia honey and isolating the related active components using organic solvents. MATERIAL AND METHODS: The crude acacia honey was extracted with n-hexane, dichloromethane, ethyl acetate (EtOAc), and n-butanol...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28805634/honey-bee-gut-dysbiosis-a-novel-context-of-disease-ecology
#7
REVIEW
Kirk E Anderson, Vincent A Ricigliano
The honey bee microbiota has become a hot-spot of recent research. Highly co-evolved with its host, the hindgut microbiota of a worker honey bee consists of six bacterial species shown to occur reliably in particular proportions. Altered microbiota structure is associated with host deficiencies, and a variety of bacteria found throughout the hive environment can dominate the worker gut suppressing or displacing microbiota function. The synthesis presented here suggests environmental insults alter gut bacterial balance, leading to decreased host function and disease progression...
August 2017: Current Opinion in Insect Science
https://www.readbyqxmd.com/read/28805554/apitherapeutics-and-phage-loaded-nanofibers-as-wound-dressings-with-enhanced-wound-healing-and-antibacterial-activity
#8
Wessam A Sarhan, Hassan Me Azzazy
AIM: Develop green wound dressings which exhibit enhanced wound-healing ability and potent antibacterial effects. METHODS: Honey, polyvinyl alcohol, chitosan nanofibers were electrospun and loaded with bee venom, propolis and/or bacteriophage against the multidrug-resistant Pseudomonas aeruginosa and examined for their antibacterial, wound-healing ability and cytotoxicity. RESULTS: Among different formulations of nanofibers, honey, polyvinyl alcohol, chitosan-bee venom/bacteriophage exhibited the most potent antibacterial activity against all tested bacterial strains (Gram-positive and -negative strains) and achieved nearly complete killing of multidrug-resistant P...
August 14, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28803879/pathway-profiles-based-on-gene-set-enrichment-analysis-in-the-honey-bee-apis-mellifera-under-brood-rearing-suppressed-conditions
#9
Kyungmun Kim, Ju Hyeon Kim, Young Ho Kim, Seong-Eui Hong, Si Hyeock Lee
Perturbation of normal behaviors in honey bee colonies by any external factor can immediately reduce the colony's capacity for brood rearing, which can eventually lead to colony collapse. To investigate the effects of brood-rearing suppression on the biology of honey bee workers, gene-set enrichment analysis of the transcriptomes of worker bees with or without suppressed brood rearing was performed. When brood rearing was suppressed, pathways associated with both protein degradation and synthesis were simultaneously over-represented in both nurses and foragers, and their overall pathway representation profiles resembled those of normal foragers and nurses, respectively...
August 10, 2017: Genomics
https://www.readbyqxmd.com/read/28800885/methyl-farnesoate-epoxidase-mfe-gene-expression-and-juvenile-hormone-titers-in-the-life-cycle-of-a-highly-eusocial-stingless-bee-melipona-scutellaris
#10
Carlos Antônio Mendes Cardoso-Júnior, Renato Pereira Silva, Naiara Araújo Borges, Washington João de Carvalho, S Leal Walter, Zilá Luz Paulino Simões, Marcia Maria Gentile Bitondi, Carlos Ueira Vieira, Ana Maria Bonetti, Klaus Hartfelder
In social insects, juvenile hormone (JH) has acquired novel functions related to caste determination and division of labor among workers, and this is best evidenced in the honey bee. In contrast to honey bees, stingless bees are a much more diverse group of highly eusocial bees, and the genus Melipona has long called special attention due to a proposed genetic mechanism of caste determination. Here, we examined methyl farnesoate epoxidase (mfe) gene expression, encoding an enzyme relevant for the final step in JH biosynthesis, and measured the hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers...
August 9, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28800099/portuguese-honeys-from-different-geographical-and-botanical-origins-a-4-year-stability-study-regarding-quality-parameters-and-antioxidant-activity
#11
Sonia Soares, Diana Pinto, Francisca Rodrigues, Rita C Alves, M Beatriz P P Oliveira
Portuguese honeys (n = 15) from different botanical and geographical origins were analysed regarding their quality parameters (diastase activity, hydroxymethylfurfural content, moisture and pH), colour (L*, a*, b*) and antioxidant profile (total phenolics content, total flavonoids content, DPPH• scavenging activity, and ferric reducing power). The samples were analysed fresh and after 4-years of storage (at 25 °C and protected from light). The hydroxymethylfurfural content and diastase activity of the fresh samples were in accordance with the recommended values described in the legislation...
August 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28797906/amitraz-and-its-metabolite-modulate-honey-bee-cardiac-function-and-tolerance-to-viral-infection
#12
Scott T O'Neal, Carlyle C Brewster, Jeffrey R Bloomquist, Troy D Anderson
The health and survival of managed honey bee (Apis mellifera) colonies are affected by multiple factors, one of the most important being the interaction between viral pathogens and infestations of the ectoparasitic mite Varroa destructor. Currently, the only effective strategy available for mitigating the impact of viral infections is the chemical control of mite populations. Unfortunately, the use of in-hive acaricides comes at a price, as they can produce sublethal effects that are difficult to quantify, but may ultimately be as damaging as the mites they are used to treat...
August 7, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28797675/from-the-hive-honey-a-novel-weapon-against-cancer
#13
REVIEW
Mariateresa Badolato, Gabriele Carullo, Erika Cione, Francesca Aiello, Maria Cristina Caroleo
Nowadays there is a folk medicine branch called apitherapy that aims to treat diseases with bee products, including honey. Honey has long been known for its medicinal and health promoting properties. It encloses numerous types of phytochemicals with high phenolic and flavonoid content, which contribute to its antioxidant and anti-inflammatory activities. Varieties and variants of polyphenols in honey showed antiproliferative property against several types of cancer. This review focuses on the latest discoveries about the key role of honey in different stages of carcinogenesis, initiation, proliferation and progression, both in vitro and in vivo, as well as on its adjuvant effect in cancer therapy...
August 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28795889/use-of-an-engineered-honey-to-eradicate-preformed-biofilms-of-important-wound-pathogens-an-in-vitro-study
#14
F D Halstead, M A Webber, B A Oppenheim
OBJECTIVE: We previously reported on the ability of SurgihoneyRO (SHRO), an engineered honey, to prevent biofilm formation in vitro, but data were lacking regarding the activity against preformed biofilms. This study aims to assess whether SHRO has any antibacterial activity against mature, preformed biofilms and whether there is any evidence to support the observed clinical effectiveness when SHRO has been used anecdotally on acute and chronic wounds where biofilm is most likely present...
August 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28795524/resin-foraging-dynamics-in-varroa-destructor-infested-hives-a-case-of-medication-of-kin
#15
Michelina Pusceddu, Giannella Piluzza, Panagiotis Theodorou, Franco Buffa, Luca Ruiu, Simonetta Bullitta, Ignazio Floris, Alberto Satta
Social insects have evolved colony behavioral, physiological and organizational adaptations (social immunity) to reduce the risks of parasitization and/or disease transmission. The collection of resin from various plants and its use in the hive as propolis, is a clear example of behavioral defense. For Apis mellifera, an increased propolis content in the hive may correspond to variations in the microbial load of the colony and to a down-regulation of an individual bee's immune response. However, many aspects of such antimicrobial mechanism still need to be clarified...
August 10, 2017: Insect Science
https://www.readbyqxmd.com/read/28794506/active-macromolecules-of-honey-form-colloidal-particles-essential-for-honey-antibacterial-activity-and-hydrogen-peroxide-production
#16
Katrina Brudzynski, Danielle Miotto, Linda Kim, Calvin Sjaarda, Liset Maldonado-Alvarez, Henryk Fukś
Little is known about the global structure of honey and the arrangement of its main macromolecules. We hypothesized that the conditions in ripened honeys resemble macromolecular crowding in the cell and affect the concentration, reactivity, and conformation of honey macromolecules. Combined results from UV spectroscopy, DLS and SEM showed that the concentration of macromolecules was a determining factor in honey structure. The UV spectral scans in 200-400 nm visualized and allowed quantification of UV-absorbing compounds in the following order: dark > medium > light honeys (p < 0...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28775197/protocol-for-a-randomised-controlled-trial-of-90-kanuka-honey-versus-5-aciclovir-for-the-treatment-of-herpes-simplex-labialis-in-the-community-setting
#17
Alex Semprini, Joseph Singer, Nicholas Shortt, Irene Braithwaite, Richard Beasley
INTRODUCTION: Worldwide, about 90% of people are infected with the herpes simplex virus, 30% of whom will experience recurrent herpes simplex labialis, commonly referred to as 'cold sores', which can last up to 10 days. The most common treatment is aciclovir cream which reduces healing time by just half a day compared with no specific treatment. This is a protocol for a randomised controlled trial (RCT) to determine the efficacy of medical grade kanuka honey-based topical treatment (Honevo) in reducing the healing time and pain of cold sores, compared with topical aciclovir treatment (Viraban)...
August 3, 2017: BMJ Open
https://www.readbyqxmd.com/read/28763989/a-modified-fox-1-method-for-micro-determination-of-hydrogen-peroxide-in-honey-samples
#18
Dan Li, Meng Wang, Ni Cheng, Xiaofeng Xue, Liming Wu, Wei Cao
Hydrogen peroxide (H2O2) is a major antibacterial activity-associated biomarker in honey. Measurement of endogenous H2O2 in honey is of great value in prediction of the H2O2-depended antibacterial activity and characterization or selection of honey samples for their use as an antibacterial agent or natural food preservative. Considering current methods for H2O2 determination are either time-consuming or complicated with their high-cost, a study was conducted to modify and validate the spectrophotometry-based ferrous oxidation-xylenol orange (FOX-1) method for micro-determination of H2O2 in honey samples...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763988/screening-bioactivity-and-bioactive-constituents-of-nordic-unifloral-honeys
#19
Anneli Salonen, Virpi Virjamo, Päivi Tammela, Laure Fauch, Riitta Julkunen-Tiitto
The objective of this study was to screen the antibacterial and antioxidant activity of thirty nine honey samples from Finland, Sweden, Norway and Denmark. Their physicochemical properties were analysed, antioxidant activity was evaluated by DPPH assay and antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus was assessed by microdilution assay. The honey samples obtained were buckwheat, caraway, clover, dandelion, fireweed, heather, lime tree, lingonberry, rape, raspberry, sweet clover, willow, mire, honeydew and polyfloral...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763958/electroanalysis-and-laccase-based-biosensor-on-the-determination-of-phenolic-content-and-antioxidant-power-of-honey-samples
#20
Jerônimo Raimundo de Oliveira Neto, Stefani Garcia Rezende, Gérman Sanz Lobón, Telma Alves Garcia, Isaac Yves Lopes Macedo, Luane Ferreira Garcia, Virgínia Farias Alves, Ieda Maria Sapateiro Torres, Mariângela Fontes Santiago, Fernando Schmidt, Eric de Souza Gil
Honey is a functional food widely consumed. Thus, the evaluation of honey samples to determine its phenolic content and antioxidant capacity (AOC) is relevant to determine its quality. Usually AOC is performed by spectrophotometric methods, which lacks reproducibility and practicality. In this context, the electroanalytical methods offer higher simplicity and accuracy. Hence, the aim of this work was to use of electroanalytical tools and laccase based biosensor on the evaluation of AOC and total phenol content (TPC) of honey samples from different countries...
December 15, 2017: Food Chemistry
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