keyword
https://read.qxmd.com/read/38138013/polyphasic-characterization-and-genomic-insights-into-an-aerobic-denitrifying-bacterium-shewanella-zhuhaiensis-sp-nov-isolated-from-a-tidal-flat-sediment
#21
JOURNAL ARTICLE
Yang Liu, Tao Pei, Juan Du, Honghui Zhu
A new, facultatively anaerobic, light-yellow, and rod-shaped bacterium designated as 3B26T isolated from Qi'ao Island's tidal flat sediment was identified. Strain 3B26T can hydrolyze gelatin, aesculin, and skim milk. The major cellular fatty acids were identified as iso-C15:0 , referred to as summed feature 3, and C16:0 ; the polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and phospholipid; and the quinones contained Q-7, Q-8, MK-7, and MMK7. The genomic size of strain 3B26T was 4,682,650 bp, and its genomic DNA G + C content was 54...
November 27, 2023: Microorganisms
https://read.qxmd.com/read/38128853/no-detectable-coagulation-activation-after-vitamin-k-mk-7-supplementation-in-patients-on-dialysis-with-functional-vitamin-k-deficiency-a-one-year-randomized-placebo-controlled-study
#22
JOURNAL ARTICLE
Else-Marie Bladbjerg, Karin Levy-Schousboe, Marie Frimodt-Møller, Krista D Kjærgaard, Charlotte Strandhave, Claus L Brasen, Niels Erik Frandsen, Ditte Hansen, Peter Marckmann
OBJECTIVE: Patients on dialysis treatment have poor functional vitamin K status, and this may increase the risk of vascular calcification. Vitamin K supplementation may therefore be relevant in patients on dialysis, but the procoagulant effects have not been studied. We evaluated effects of menaquinone-7 (MK-7) supplementation on biomarkers of coagulation in patients on dialysis. DESIGN AND METHODS: Double-blinded, placebo-controlled study in 123 patients on dialysis randomized to 52 weeks of vitamin K (MK-7, 360 μg/daily, n=61) or placebo (n=62)...
December 19, 2023: Journal of Renal Nutrition
https://read.qxmd.com/read/38108591/-antiquaquibacter-oligotrophicus-gen-nov-sp-nov-a-novel-oligotrophic-bacterium-from-groundwater
#23
JOURNAL ARTICLE
Marwene Toumi, William B Whitman, Nikos C Kyrpides, Tanja Woyke, Jacqueline Wolf, Meina Neumann-Schaal, Gorkhmaz Abbaszade, Bóka Károly, Erika Tóth
In this study, a Gram-stain-positive, non-motile, oxidase- and catalase-negative, rod-shaped, bacterial strain (SG_E_30_P1T ) that formed light yellow colonies was isolated from a groundwater sample of Sztaravoda spring, Hungary. Based on 16S rRNA phylogenetic and phylogenomic analyses, the strain was found to form a distinct linage within the family Microbacteriaceae . Its closest relatives in terms of near full-length 16S rRNA gene sequences are Salinibacterium hongtaonis MH299814 (97.72 % sequence similarity) and Leifsonia psychrotolerans GQ406810 (97...
December 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38097527/-brevibacillus-ruminantium-sp-nov-isolated-from-cow-faeces
#24
JOURNAL ARTICLE
Seunghwan Kim, Jun Heo, Soon-Wo Kwon, Daseul Lee, Byeong-Hak Han, Seung-Beom Hong, Yiseul Kim
An aerobic, Gram-stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3-R1T , was isolated from cow faeces sampled in Daejeon, Republic of Korea. Growth was observed at 25-45 °C (optimum, 35-40 °C) and pH 7.0-9.0 (optimum, pH 8.0), with up to 3 % (w/v) NaCl (optimum, 0 % NaCl). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain BB3-R1T to Brevibacillus borstelensis NRRL NRS-818T (98.8 %) followed by Brevibacillus panacihumi JCM 15085T (97...
December 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38079210/-marivirga-aurantiaca-sp-nov-a-halophilic-nitrite-reducing-bacterium-isolated-from-intertidal-surface-sediments
#25
JOURNAL ARTICLE
Mingxia Zhang, Yulian Zhang, Qing Yao, Fan Yang, Honghui Zhu
A novel Gram-stain-negative strain, designated S37H4T , was isolated from an intertidal surface sediment sample collected from Zhanjiang City, Guangdong province, south PR China. Cells of the strain were aerobic, non-flagellated, long rod-shaped and motile by gliding. S37H4T could grow at 4-40 °C, pH 7.0-8.5 and in 2.0-15.0 % NaCl, with optimal growth at 25-30 °C, pH 7.5 and 9.0 % NaCl, respectively. S37H4T was capable of nitrite removal under high-salt conditions, and there were three denitrification genes, nirK , norB and nosZ, in its genome...
December 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38043166/analysis-of-vitamin-k-1-and-major-k-2-variants-in-rat-human-serum-and-lipoprotein-fractions-by-a-rapid-simple-and-sensitive-uhplc-ms-ms-method
#26
JOURNAL ARTICLE
Kristýna Mrštná, Kateřina Matoušová, Lenka Kujovská Krčmová, Alejandro Carazo, Jana Pourová, Přemysl Mladěnka, Ludmila Matysová, František Švec
Determination of the various forms of vitamin K, which are involved in coagulation and other physiological processes in humans, is challenging and no standardized method is yet available. Therefore, a reliable and practical method was developed to quantify vitamin K levels in serum and additionally in lipoprotein fractions to clarify its distribution. The LC-MS/MS method for the determination of vitamin K1 and the three main isoforms of vitamin K2 (MK-4, MK-7, MK-9) was combined with a gradient ultracentrifugation technique to allow the separation of lipoprotein fractions...
November 29, 2023: Journal of Chromatography. A
https://read.qxmd.com/read/38015052/-paenibacillus-polygoni-sp-nov-an-endophytic-bacterium-isolated-from-polygonum-lapathifolium-l-in-wetland
#27
JOURNAL ARTICLE
Yong Long, Yansheng Li, Guanghua Wang, Jian Jin, Mengfan Mao, Lizheng Gao, Guihua Liu, Guoquan Fan, Zhenhua Yu
A Gram-stain-positive, aerobic, rod-shaped, non-motile, yellowish and glossy strain, C31T , was isolated from a wetland plant Polygonum lapathifolium L. located south of Poyang Lake, Jiangxi Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C31T showed similarity values of lower than 97.0 % to other type species belonging to the genus Paenibacillus . The genomic average nucleotide identity values between strain C31T and its reference type species ranged from 68...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38011192/vitamin-k2-mk-7-attenuates-lps-induced-acute-lung-injury-via-inhibiting-inflammation-apoptosis-and-ferroptosis
#28
JOURNAL ARTICLE
Yulian Wang, Weidong Yang, Lulu Liu, Lihong Liu, Jiepeng Chen, Lili Duan, Yuyuan Li, Shuzhuang Li
Acute lung injury (ALI) is a life-threatening disease that has received considerable critical attention in the field of intensive care. This study aimed to explore the role and mechanism of vitamin K2 (VK2) in ALI. Intraperitoneal injection of 7 mg/kg LPS was used to induce ALI in mice, and VK2 injection was intragastrically administered with the dose of 0.2 and 15 mg/kg. We found that VK2 improved the pulmonary pathology, reduced myeloperoxidase (MPO) activity and levels of TNF-α and IL-6, and boosted the level of IL-10 of mice with ALI...
2023: PloS One
https://read.qxmd.com/read/38004738/pan-genome-analyses-of-the-genus-cohnella-and-proposal-of-the-novel-species-cohnella-silvisoli-sp-nov-isolated-from-forest-soil
#29
JOURNAL ARTICLE
Chunling Wang, Lutian Mao, Gegen Bao, Honghui Zhu
Two strains, designated NL03-T5T and NL03-T5-1, were isolated from a soil sample collected from the Nanling National Forests, Guangdong Province, PR China. The two strains were Gram-stain-positive, aerobic, rod-shaped and had lophotrichous flagellation. Strain NL03-T5T could secrete extracellular mucus whereas NL03-T5-1 could not. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains belong to the genus Cohnella , were most closely related to Cohnella lupini LMG 27416T (95.9% and 96...
November 8, 2023: Microorganisms
https://read.qxmd.com/read/37991220/-shiella-aurantiaca-gen-nov-sp-nov-a-novel-bacterium-isolated-from-activated-sludge-and-proposal-of-shiellaceae-fam-nov
#30
JOURNAL ARTICLE
Bin Liu, Yingying Wan, Le Liu, Yu Lv, Qi Zhang, Dan Cheng, Jian He, Qirong Shen
A novel Gram-stain-negative, long rod-shaped, aerobic, non-motile, non-spore-forming and orange-pigmented bacterium, designated LB-30T , was isolated from activated sludge. Growth was observed at 15-40 °C (optimum 30 °C), pH 6.0-9.0 (optimum 7.0) and salinities of 0-3.0 % (w/v; optimum 2.0 %). LB-30T showed less than 89.9 % sequence similarities to the recognized taxa of the order Cytophagales . The results of phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic tree indicated that LB-30T formed a distinct lineage within the order Cytophagales ...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37982814/-paenibacillus-caseinilyticus-sp-nov-isolated-forest-soil
#31
JOURNAL ARTICLE
Hyosun Lee, Dhiraj Kumar Chaudhary, Oung Bin Lim, Ki Eun Lee, In Tae Cha, Won Jae Chi, Dong-Uk Kim
A milky-white-coloured, aerobic, Gram-stain-positive, rod-shaped and motile bacterial strain (GW78T ) was isolated from forest soil. GW78T was catalase-positive and oxidase-negative. The strain was able to grow optimally at 37 °C and at pH 7.0 in Reasoner's 2A media. The phylogenetic and 16S rRNA gene sequence analysis of GW78T showed its affiliation with the genus Paenibacillus . The 16S rRNA gene sequence of GW78T revealed 98.3 % similarity to its nearest neighbour Paenibacillus mucilaginosus VKPM B-7519T ...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37943169/-lysinibacillus-irui-sp-nov-isolated-from-iru-fermented-african-locust-beans
#32
JOURNAL ARTICLE
Stephen Olusanmi Akintayo, Bernd Neumann, Martin Fischer, Marius Henkel, Lars Lilge, Rudolf Hausmann
A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01T was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01T grew at 10-50 °C (optimum, 35-37 °C), pH 6-10 (optimum, pH 7) and in 0-6 % NaCl (optimum, 1-3 %). Phylogenetic analyses based on 16S rRNA and combined short- and long-read genome sequencing revealed that IRB4-01T is closely related to Lysinibacillus cavernae SYSU K30005T and Lysinibacillus boronitolerans 10aT ...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37942742/-robiginitalea-aurantiaca-sp-nov-and-algoriphagus-sediminis-sp-nov-isolated-from-coastal-sediment
#33
JOURNAL ARTICLE
Zi-Yang Zhou, Jing An, Ya-Wei Jia, Xiao-Qi Xuan, Zong-Jun Du
Two novel rod-shaped, Gram-stain-negative, aerobic and non-motile bacterial strains, designated M39T and C2-7T , were isolated from the coastal sediment of Xiaoshi Island, Weihai, PR China. Growth of strain M39T occurred at 15-37 °C, at pH 6.0-9.0 and in the presence of 1.0-9.0 % (w/v) NaCl. Strain C2-7T grew at 15-40 °C, at pH 6.0-8.0 and in the presence of 0.5-8.0 % (w/v) NaCl. Phylogenetic analysis based 16S rRNA gene sequences revealed that strains M39T and C2-7T belong to the phylum Bacteroidota ...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37930394/aquibacillus-rhizosphaerae-sp-nov-an-indole-acetic-acid-iaa-producing-halotolerant-bacterium-isolated-from-the-rhizosphere-soil-of-kalidium-cuspidatum
#34
JOURNAL ARTICLE
Wen-Jing Ding, Lian Xu, Yang Zhao, Ji-Quan Sun
A bacterium (named strain LR5S19T ) was isolated from the rhizosphere soil of the halophyte Kalidium cuspidatum in Baotou, Inner Mongolia, China. Strain LR5S19T was Gram-stain-positive, motile with a polar flagellum, rod shaped, and spore forming at the terminal position in swollen sporangia, and it grew at 10-40 ℃ (optimum 30 ℃), pH 6.0-9.0 (optimum pH 7.0), and in the presence of 1.0-15.0% (w/v) NaCl (optimum 2.0%). The phylogenetic analysis of the 16S rRNA gene showed that strain LR5S19T shared the highest similarity (96...
November 6, 2023: Current Microbiology
https://read.qxmd.com/read/37921447/two-novel-alkalitolerant-species-pseudalkalibacillus-spartinae-sp-nov-and-pseudalkalibacillus-sedimenti-sp-nov
#35
JOURNAL ARTICLE
Guo-Hong Liu, Shuang Han, Bing Li, Rui-Li Li, Huai Shi, Qian-Qian Chen, Hend A Alwathnani, Christopher Rensing, Shun-Gui Zhou
In this study, two novel alkalitolerant strains (FJAT-53046T and FJAT-53715T ) were isolated from sediment samples collected in Zhangzhou, PR China. Phylogeny based on 16S rRNA gene sequences suggested that strains FJAT-53046T and FJAT-53715T were new members of the genus Pseudalkalibacillus . The two novel strains showed the highest 16S rRNA gene sequence similarity to Pseudalkalibacillus hwajinpoensis DSM 16206T , with values of 97.4 and 97.6 %, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains and the reference strain were 77...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37917138/-pontibacter-harenae-sp-nov-isolated-from-the-soil-of-a-populus-euphratica-forest
#36
JOURNAL ARTICLE
Ghenijan Osman, Gulnisa Sayim, Hongmei Yang, Meiying Gu, Jian Sun, Yan Gao, Jing Zhu, Suqin Song, Yuanyang Yi, Wei Wang, Zhidong Zhang
A Gram-negative, rod-shaped, aerobic and light pink-pigmented bacterium, designated XAAS-A31T , was isolated from the soil of a Populus euphratica forest located near Hotan City, Xinjiang, PR China. Polyphasic, taxonomic and phylogenomic analyses were used to determine the taxonomy position of the strain. Phylogenetic analysis based on 16S rRNA gene sequence analysis indicated that XAAS-A31T belongs to the genus Pontibacter , family Hymenobacteraceae , and shows highest sequence similarity to Pontibacter silvestris XAAS-R86T (96...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37916707/-lacibacter-sediminis-sp-nov-isolated-from-contaminated-freshwater-sediment
#37
JOURNAL ARTICLE
Ye Zhuo, Chun-Zhi Jin, Feng-Jie Jin, Hee-Mock Oh, Hyung-Gwan Lee, Taihua Li, Long Jin
Two Gram-stain-negative bacterial strains, S13-6-6 and S13-6-22T , were isolated from sediment sample collected at a water depth of 4 m from Lake Hongze, Jiangsu Province, PR China. The cells of strains S13-6-6 and S13-6-22T were non-spore-forming, aerobic, non-motile and formed orange colonies on R2A agar. Comparative 16S rRNA gene sequence studies revealed a clear affiliation of the two strains with he phylum Bacteroidota , and revealed the highest pairwise sequence similarities with Lacibacter daechungensis H32-4T (97...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37910170/-paenibacillus-spongiae-sp-nov-isolated-from-deep-water-marine-sponge-theonella-swinhoei
#38
JOURNAL ARTICLE
Mimi Zhang, Qianqian Song, Jin Sang, Zhiyong Li
A novel bacterial strain, designated as PHS-Z3T , was isolated from a marine sponge belonging to the genus Theonella on the Puerto Galera Deep Monkey, Philippines. Cells of PHS-Z3T were Gram-stain-positive, motile, oxidase- and catalase-positive, white-pigmented, spore-forming, short rods that could grow at 10-40 °C (optimum, 20 °C), pH 6.0-9.5 (optimum, pH 7.5) and with 2-16 % (w/v) NaCl (optimum, 7 %). The 16S rRNA gene sequence of PHS-Z3T showed 97.9 %, 96.7 %, and 96...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37889152/-aurantibacillus-circumpalustris-gen-nov-sp-nov-the-first-characterized-representative-of-the-bacteroidota-candidate-family-env-ops-17-and-proposal-of-aurantibacillaceae-fam-nov
#39
JOURNAL ARTICLE
Selma Vieira, Thomas Riedel, Alicia Geppert, Manfred Rohde, Jacqueline Wolf, Meina Neumann-Schaal, Jörg Overmann
16S rRNA sequence types associated with the candidate family env.OPS 17 have been reported from various environments, but no representatives have been characterized and validly named. Bacteria of env.OPS 17 are affiliated with the order Sphingobacteriales and were first detected more than two decades ago in the vicinity of a thermal spring in Yellowstone National Park. Strain Swamp196T , isolated from the soil surrounding a swamp in Northern Germany, is the first characterized representative of candidate family env...
October 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37882660/-halobacillus-salinarum-sp-nov-halobacillus-shinanisalinarum-sp-nov-and-halobacillus-amylolyticus-sp-nov-isolated-from-saltern-soil
#40
JOURNAL ARTICLE
Yiseul Kim, Seunghwan Kim, Soon-Wo Kwon, Hang-Yeon Weon, Hanako Naito, Tomomi Asano, Moriyuki Hamada, Jun Heo
Three bacterial strains, designated SSBR10-3T , SSTM10-2T and SSHM10-5T , were isolated from saltern soil sampled in Jeollanam-do, Republic of Korea. Cells were aerobic, Gram-stain-positive, flagellated and rod-shaped. The strains grew optimally at 28°C and at pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains SSBR10-3T , SSTM10-2T and SSHM10-5T were placed within the genus Halobacillus , showing the highest similarity to Halobacillus alkaliphilus FP5T (98.6 %), ' Halobacillus ihumii ' Marseille-Q1234T (98...
October 2023: International Journal of Systematic and Evolutionary Microbiology
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