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https://www.readbyqxmd.com/read/29333616/determination-of-vitamins-k1-mk-4-and-mk-7-in-human-serum-of-postmenopausal-women-by-hplc-with-fluorescence-detection
#1
Eva Klapkova, Jana Cepova, Katerina Dunovska, Richard Prusa
BACKGROUND: New high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K1 and two forms of vitamin K2 (MK-4 and MK-7) in human serum, and the levels of vitamin K were determined in 350 samples of postmenopausal women. METHODS: Vitamin K was determined by HPLC with fluorescence detection after postcolumn zinc reduction. The detection was performed at 246 nm (excitation) and 430 nm (emission). The internal standard and 2 mL of ethanol were added to 500 μL of serum...
January 15, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29300160/planococcus-salinus-sp-nov-a-moderately-halophilic-bacterium-isolated-from-a-saline-alkali-soil
#2
Longzhan Gan, Heming Zhang, Jiewei Tian, Xiaoguang Li, Xiufeng Long, Yuqin Zhang, Yumei Dai, Yongqiang Tian
A novel aerobic, Gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated LCB217T, was isolated from a saline-alkali soil in north-western China and identified using a polyphasic taxonomic approach. Growth occurred with 3-15 % (w/v) NaCl (optimum 3-5 %), at 10-45 °C (optimum 30 °C) and at pH 7.0-9.0 (optimum pH 9.0). Strain LCB217T contained MK-7 and MK-8 as the predominant menaquinones and anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0 as the major fatty acids...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29300151/bacillus-camelliae-sp-nov-isolated-from-pu-er-tea
#3
Lili Niu, Mengjie Xiong, Juan Zhang, Yangquan Xiang, Lei Song, Ziyi Hua, Wenying Li
A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %). The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29299848/kurthia-ruminicola-sp-nov-isolated-from-the-rumen-contents-of-a-holstein-cow
#4
Myung Kyum Kim, Eun Tae Kim, Sang Bum Kim, Ha Yeon Jeong, Beom Young Park, Sathiyaraj Srinivasan
Gram-staining-positive, motile, rod-shaped bacteria, designated as H31022T and H31024 was isolated from rumen contents of a Holstein cow. Optimum growth occurred at 25°C and pH 7.0 on R2A agar medium. Oxidase and catalase activities are positive. The 16S rRNA gene sequence (1,452 bp) of the new isolates revealed they belong to the genus Kurthia of the phylum Firmicutes. Highest gene sequence similarities were assessed to be with Kurthia massiliensis JC30T (98.4%), Kurthia senegalensis JC8ET (97.5%), and Kurthia populi 10y-14T (97...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29299847/baekduia-soli-gen-nov-sp-nov-a-novel-bacterium-isolated-from-the-soil-of-baekdu-mountain-and-proposal-of-a-novel-family-name-baekduiaceae-fam-nov
#5
Dong-Shan An, Muhammad Zubair Siddiqi, Kyoung-Ho Kim, Hong-Shan Yu, Wan-Taek Im
A taxonomic study was conducted on BR7-21T, a bacterial strain isolated from the soil of a ginseng field in Baekdu Mountain. Comparative studies of the 16S rRNA gene sequence showed that the isolate was most closely related to Conexibacter woesei DSM 14684T, Solirubrobacter pauli ATCC BAA-492T, Patulibacter minatonensis JCM 12834T, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order Solirubrobacterales. Strain BR7-21T was Gram-reaction positive, non-spore forming, aerobic, non-motile, and short rod-shaped...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29299846/larkinella-roseus-sp-nov-a-species-of-the-family-cytophagaceae-isolated-from-beach-soil
#6
Jae-Bong Lee, Sumin Hong, Seung-Yeol Lee, Su-Jin Park, Kyeung Il Park, Seok-Gwan Choi, Myung Kyum Kim, Leonid N Ten, Hee-Young Jung
The taxonomic position of bacterial strain, designated 15J16-1T3AT, recovered from a soil sample was established using a polyphasic approach. Phylogenic analysis based on the 16S rRNA gene sequence showed that strain 15J16-1T3AT belonged to the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to 'Larkinella harenae' 15J9-9 (95.9% similarity), Larkinella ripae 15J11-11T (95.6%), Larkinella bovis M2TB15T (94.7%), Larkinella arboricola Z0532T (93.9%), and Larkinella insperata LMG 22510T (93...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29297846/bacillus-tamaricis-sp-nov-an-alkaliphilic-bacterium-isolated-from-a-tamarix-cone-soil
#7
Yong-Guang Zhang, Xing-Kui Zhou, Jian-Wei Guo, Min Xiao, Hong-Fei Wang, Yun Wang, Khursheda Bobodzhanova, Wen-Jun Li
A Gram-stain-positive, alkaliphilic bacterium, designated EGI 80668T, was isolated from a Tamarix cone soil in Xinjiang, north-west China. Cells were facultatively anaerobic, terminal endospore-forming and motile by means of peritrichous flagella. Colonies were yellowish and the cells showed oxidase-negative and catalase-positive reactions. Strain EGI 80668T grew at pH 8.0-10.0 and with 0-10 % (w/v) NaCl (optimally at pH 9.0 and with 1-2 % NaCl) on marine agar 2216. The predominant menaquinone was MK-7...
January 3, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29289978/aeromicrobium-panacisoli-sp-nov-isolated-from-soil-of-ginseng-cultivating-field
#8
Muhammad Zubair Siddiqi, Soon Youl Lee, Kang Duk Choi, Wan-Taek Im
A Gram-positive, rod-shaped, non-spore-forming, and aerobic bacterium (Gsoil 137T) was isolated from soil of a ginseng field of Pocheon province in South Korea and subjected to a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 137T was shown to belong to the family Nocardioidaceae and was closely related to Aeromicrobium ginsengisoli Gsoil 098T (96.7%), Aeromicrobium panaciterrae (96.7%), and Aeromicrobium halocynthiae JCM 15749T (96...
December 30, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29251590/spirosoma-metallilatum-sp-nov-isolated-from-an-automotive-air-conditioning-system
#9
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, Jong-Ok Ka
A Gram-stain-negative, aerobic, rod-shaped and yellow-pigmented bacterial strain, designated TX0405T, was isolated from an automotive air conditioning system. Colonies were circular, convex, semi-translucent, smooth and yellow. The strain grew at 20-28°C (optimum, 28°C), at pH 6.0-7.5 (optimum, pH 6.5) and in the presence of 0-1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain was grouped with the members of the genus Spirosoma, with the sequence similarities of 93...
December 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29235981/paenibacillus-limicola-sp-nov-isolated-from-tidal-flat-sediment
#10
Shamsun Nahar, Chang-Jun Cha
An aerobic, Gram-staining-variable, rod-shaped, endospore-forming and motile bacterial strain, designated CJ6T, was isolated from a tidal flat on Ganghwa Island, South Korea. The isolate was characterized based on a polyphasic taxonomy approach. Strain CJ6T grew optimally on R2A agar media at 30 °C and pH 7. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ6T belonged to the genus Paenibacillus, displaying the highest sequence similarity to Paenibacillus vulneris CCUG 53270T (97...
December 13, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29231155/pedobacter-quisquiliarum-sp-nov-isolated-from-activated-sludge
#11
Bo Zhang, Zhi-Qiang Liu, Yu-Guo Zheng
A Gram-stain-negative, aerobic, rod-shaped, bacterium, C62-2T, was isolated from activated sludge in Fujian Province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that it was closely related to Pedobacter duraquae WB 2.1-25T (97.92 %), Pedobacter bambusae THG-G118T (97.40 %), Pedobacter cryoconitis A37T (97.37 %) and Pedobacter caeni LMG 22862T (97.3 %). Cells grew aerobically at 20-37 °C (optimum, 30 °C), pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-3.0 % (w/v) NaCl...
December 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29219806/mucilaginibacter-amnicola-sp-nov-isolated-from-a-freshwater-creek
#12
Wen-Ming Chen, Tsai-Ying Hsieh, Shih-Yi Sheu
A pink-coloured bacterial strain, TAPP7T, was isolated from a freshwater creek in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TAPP7T belonged to the genus Mucilaginibacter and showed highest similarity with Mucilaginibacter ginsengisoli B4Y-8T (97.6 %) and Mucilaginibacter carri PR0008KT (96.9 %). Cells of strain TAPP7T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating and short-rod-shaped. Growth occurred at 10-30 °C (optimum, 15-20 °C), at pH 4-8 (optimum, pH 6) and with 0-1 % NaCl (optimum, 0...
December 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29214490/spirosoma-migulaei-sp-nov-isolated-from-soil
#13
Joseph Okiria, Leonid N Ten, Su-Jin Park, Seung-Yeol Lee, Dong Hoon Lee, In-Kyu Kang, Dae Sung Lee, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-8T, was isolated from soil on Jeju Island, Republic of Korea. The isolate was able to grow between 10 and 30°C, pH 6.5-8.5, and in presence of 0-1% (w/v) NaCl. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J9-8T represented a member of the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to Spirosoma aerophilum 5516J-17T (96.1% similarity), Spirosoma pulveris JSH5-14T (95...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29209820/spirosoma-terrae-sp-nov-isolated-from-soil-from-jeju-island-korea
#14
Leonid N Ten, Joseph Okiria, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, In-Kyu Kang, Myung Kyum Kim, Hee-Young Jung
The taxonomic position of bacterial strain, designated 15J9-4T, recovered from a beach soil sample on Jeju Island, South Korea, was established using a polyphasic approach. Strain 15J9-4T was assigned to phylum Bacteroidetes within the family Cytophagaceae based on 16S rRNA gene similarities. The closest phylogenetic relatives with validly published names were Spirosoma panaciterrae Gsoil 1519T (94.2% similarity) and Spirosoma luteolum 16F6ET (94.1%). Cells were rod-shaped, Gram-stain-negative, and non-motile...
December 5, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29205133/pontibacter-brevis-sp-nov-isolated-from-rhizosphere-soil-of-tamarix-ramosissima
#15
Ghenijan Osman, Yan Gao, Ning Wang, Otkur Mahmud, Jian Sun, Tao Zhang, Faqiang Zhan, Zhidong Zhang, Kai Lou
A Gram-stain-negative, oval-shaped and light pink pigmented bacterium, designated XAAS-2T, was isolated from rhizosphere soil of Tamarix ramosissima. The sole respiratory quinone of the type strain XAAS-2T was MK-7, and the principal cellular fatty acids were summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C15 : 0. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. 16S rRNA gene sequence analysis indicated that strain XAAS-2T belonged to the genus Pontibacter within the family Cytophagaceae with sequence similarities of 93...
December 5, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29188342/spirosoma-jeollabukense-sp-nov-isolated-from-soil
#16
Weilan Li, Leonid N Ten, Seung-Yeol Lee, Dong Hoon Lee, Hee-Young Jung
A Gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated S2-3-6T, was isolated from soil in Jeollabuk-do province, South Korea, and was characterized taxonomically using a polyphasic approach. According to a comparative 16S rRNA gene sequence analysis, strain S2-3-6T belonged to the family Cytophagaceae and was most closely related to Spirosoma endophyticum EX36T (98.2%), Spirosoma fluviale MSd3T (96.0%), and Spirosoma linguale DSM 74T (95.7%). Strain S2-3-6T contained summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:1 ω5c, and C16:0 N alcohol as major cellular fatty acids, MK-7 as the predominant respiratory quinone, and phosphatidylethanolamine as the main polar lipid...
November 29, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29185117/algoriphagus-formosus-sp-nov-isolated-from-coastal-sediment
#17
Meng-Qi Ye, Xu-Ting Wang, Jing Zhang, Guan-Jun Chen, Zong-Jun Du
A Gram-stain negative, facultative anaerobic, non-motile, strongly orange-pigmented and rod-shaped bacterium, designated XAY3209T, was isolated from a marine sediment sample collected from the coast of Weihai, China. Strain XAY3209T was found to grow optimally at 30 °C, at pH 7.0 and in the presence of 2.0% (w/v) NaCl. Its genomic DNA G+C content was 41.9 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the family Cyclobacteriaceae and is related to the genus Algoriphagus...
November 28, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29184975/gracilibacillus-eburneus-sp-nov-a-moderately-halophilic-bacterium-isolated-from-xinjiang-province-china
#18
Hui-Lin Guan, Yun-Jiao Zhang, Xiao-Ju Lu, Man Jia, Zheng-Yun Zhang, Xu-Hong Gao, Yong-Chun Ma, Fei Tian, Shu-Kun Tang
A novel Gram-staining positive, moderately halophilic, endospore-forming, motile, rod-shaped and strictly aerobic strain, designated YIM 93565T, was isolated from a salt lake in Xinjiang province of China and subjected to a polyphasic taxonomic study. Strain YIM 93565T grew in the range of pH 6.0-9.0 (optimum pH 7.0), 10-45 °C (optimum 35-40 °C) and at salinities of 2-24% (w/v) NaCl (optimum 7-10%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 93565T clustered with members of the genera Gracilibacillus and form a clade with Gracilibacillus bigeumensis KCTC 13130T (95...
November 28, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29148364/salibacterium-lacus-sp-nov-a-halophilic-non-spore-forming-bacterium-isolated-from-sediment-of-a-saline-lake
#19
Yue-Qiang Wang, Xiao-Ming Liu, Jia Tang, Gui-Qin Yang, Zhen Yu
A novel halophilic bacterium, strain GSS13(T), capable of growing at salinities of 8-28 % (w/v) NaCl (optimally at 24 %, w/v) was isolated from Yuncheng Saline Lake in China. GSS13(T) was Gram-stain-positive, strictly aerobic, rod-shaped, motile and a non-spore-former. Growth occurred at pH 5.5-8.5 (optimum pH 7.0) and at 10-45 °C (optimum 30 °C). On the basis of the results of 16S rRNA gene sequences phylogenetic analyses, GSS13(T) represents a member of the genus Salibacterium and is closely related to Salibacterium halotolerans S7(T), Salibacterium qingdaonense CM1(T) and Salibacterium halochares MSS4(T), with 16S rRNA gene sequence similarities of 98...
November 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29148363/hymenobacter-defluvii-sp-nov-isolated-from-wastewater-of-an-acidic-water-neutralization-facility
#20
Joo Won Kang, Seon Choi, Han Na Choe, Chi Nam Seong
A non-motile, pink-coloured and rod-shaped bacterium, designated strain POA9(T), was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-reaction-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were C16 : 1ω5c, iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and anteiso-C15 : 0. The strain contained MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine...
November 17, 2017: International Journal of Systematic and Evolutionary Microbiology
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