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https://www.readbyqxmd.com/read/28913006/uhplc-pda-assay-for-simultaneous-determination-of-vitamin-d3-and-menaquinone-7-in-pharmaceutical-solid-dosage-formulation
#1
Muhammad Jehangir, Mahmood Ahmed, Muhammad Imtiaz Shafiq, Abdul Samad, Iftikhar-Ul-Haq
A newly developed method based on ultrahigh performance liquid chromatography (UHPLC) was optimized for the simultaneous determination of vitamin D3 and menaquinone-7 (MK-7) in tablet formulation in the present study. UHPLC separation of vitamin D3 and MK-7 was performed with ACE Excel 2 C18-PFP column (2 μm, 2.1 × 100 mm) at 0.6 mL min(-1) flow rate, whereas the mobile phase consisted of methanol/water (19 : 1, v/v, phase A) and isopropyl alcohol (99.9%, phase B) containing 0.5% triethylamine. Isocratic separation of both the analytes was performed at 40°C by pumping the mobile phases A and B in the ratio of 50 : 50 (v/v, pH, 6...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28905708/williamwhitmania-taraxaci-gen-nov-sp-nov-a-proteolytic-anaerobe-with-a-novel-type-of-cytology-from-lake-untersee-in-antarctica-description-of-williamwhitmaniaceae-fam-nov-and-emendation-of-the-order-bacteroidales-krieg-2012
#2
Elena V Pikuta, Zhe Lyu, Richard B Hoover, Yuchen Liu, Nisha B Patel, Hans J Busse, Paul A Lawson
The proteolytic bacterium strain A7P-90mT was isolated from Lake Untersee, Antarctica. The anoxic water was collected from a perennially sealed (~100 millennia) glacial ice lake. Gram-stain-negative cells were 0.18-0.3×8.0-25.0 µm in size, straight, slender rods with unusual gliding motility by external, not previously reported, organelles named here as antiae. At the end of stationary phase of growth, spheroplasts were terminally formed and the cells resembled dandelions. After death, cells were helical...
September 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28905705/mucilaginibacter-terrae-sp-nov-isolated-from-antarctic-soil
#3
Ivo Sedláček, Roman Pantůček, Stanislava Králová, Ivana Mašlaňová, Pavla Holochová, Eva Staňková, Roman Sobotka, Miloš Barták, Hans-Jürgen Busse, Pavel Švec
A bacterial strain designated CCM 8645T was isolated from a soil sample collected nearby a mummified seal carcass in the northern part of James Ross Island, Antarctica. The cells were short rods, Gram-stain-negative, non-motile, catalase and oxidase positive, and produced a red-pink pigment on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, extensive biotyping using conventional tests and commercial identification kits and chemotaxonomic analyses were applied to clarify its taxonomic position...
September 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28905704/nesterenkonia-pannonica-sp-nov-a-novel-alkaliphilic-and-moderately-halophilic-actinobacterium
#4
Andrea K Borsodi, Tibor Szili-Kovács, Peter Schumann, Cathrin Spröer, Károly Márialigeti, Erika Tóth
An alkaliphilic and moderately halophilic bacterial strain characterized by optimal growth at pH 9.0-10.0 and with 5-7 % (w/v) NaCl, designated BV-35T, was isolated from water of a soda pan located in Kiskunság National Park, Hungary. Cells of the orange-pigmented colony were Gram-stain-positive, non-motile and non-endospore-forming coccoid rods. The isolate was strictly aerobic, catalase-positive and oxidase-negative. Strain BV-35T displayed a peptidoglycan similar to type A4α, l-Lys-l-Glu (A11.54 according to www...
September 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28905696/paralkalibacillus-indicireducens-gen-nov-sp-nov-an-indigo-reducing-obligate-alkaliphile-isolated-from-indigo-fermentation-liquor-used-for-dyeing
#5
Kikue Hirota, Masatoshi Nishita, Hidetoshi Matsuyama, Isao Yumoto
Obligately alkaliphilic, indigo-reducing strains, designated Bps-1T, Bps-2 and Bps-3, were isolated from an indigo fermentation liquor used for dyeing, which was produced from sukumo (composted Polygonum indigo leaves) obtained from a craft centre in Data City, Hokkaido, Japan, by using medium containing cellulase-treated sukumo. The 16S rRNA gene sequence phylogeny suggested that Bps-1T has a distinctive position among the alkaliphilic species of the genus Bacillus, with its closest neighbours being Bacillus pseudofirmus DSM 8715T, Bacillus lindianensis DSM 26864T and Bacillus alcalophilus DSM 485T (96...
September 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28901904/chryseomicrobium-deserti-sp-nov-isolated-from-desert-soil-in-south-korea
#6
Pei Lin, Zheng-Fei Yan, Chang-Tian Li, MooChang Kook, Qi-Jun Wang, Tae-Hoo Yi
A Gram-stain positive, aerobic, non-motile, rod-shaped bacterium (THG-T1.18T) was isolated from desert soil. Growth occurred at 20-35 °C (optimum 28-30 °C), at pH 5-7 (optimum 7) and at 0-4 % NaCl (optimum 0-1 %). Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T1.18T were identified as Chryseomicrobium amylolyticum DSM 23442T (96.6 %), Chryseomicrobium imtechense JCM 16573T (96.3 %) and Chryseomicrobium aureum KACC 17219T (96.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and one unidentified glycolipid...
September 13, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28901897/hymenobacter-frigidus-sp-nov-isolated-from-a-glacier-ice-core
#7
Zhengquan Gu, Yongqin Liu, Baiqing Xu, Ninglian Wang, Nianzhi Jiao, Liang Shen, Hongcan Liu, Yuguang Zhou, Xiaobo Liu, Jiule Li, Jia Sun
A psychrophilic, Gram-stain-negative, rod-shaped, red-pigmented bacterium, designated strain B1789T, was isolated from an ice core of Muztagh Glacier on the Tibetan Plateau in China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B1789T was related to members of the genus Hymenobacter and had highest sequence similarity with Hymenobacter antarcticus JCM 17217T (97.9 %). The major menaquinone was MK-7 and the major polar lipid was phosphatidylethanolamine. The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c)...
September 13, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28895519/parapedobacter-lycopersici-sp-nov-isolated-from-the-rhizosphere-soil-of-tomato-plants-solanum-lycopersicum-l
#8
Jeong Myeong Kim, Shin Ae Lee, Hayoung Cho, Soo-Jin Kim, Jae-Ho Joa, Soon-Wo Kwon, Hang-Yeon Weon
A novel Gram-stain-negative bacterial strain, designated T16R-256T, was isolated from the rhizosphere soil of tomato plants grown in a greenhouse in Yecheon-gun, Gyeongsangbuk-do, Republic of Korea and characterized using polyphasic taxonomy. Cells were aerobic, non-flagellated and rod-shaped. Colonies were light yellow, convex and round. The strain grew in the temperature range of 15-37 °C (optimally at 28-30 °C) and pH range of 7.0-9.0 (optimally at 7.0-8.0) and in 4 % NaCl (w/v). A comparison of 16S rRNA gene sequences showed that strain T16R-256T is a member of the genus Parapedobacter, exhibiting high sequence similarity with Parapedobacter pyrenivorans P-4T (94...
September 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28895514/lentibacillus-sediminis-sp-nov-isolated-from-a-marine-saltern
#9
Ling-Yun Guo, Nan-Nan Wang, Xiao-Qun Wang, Guan-Jun Chen, Zong-Jun Du
A novel, Gram-stain-positive, moderately halophilic, endospore-forming, motile, facultatively anaerobic and rod-shaped strain, designated 0W14T, was isolated from a marine saltern of Wendeng, China. Optimal growth occurred at 37 °C, pH 7.5 and with 6.0 % (w/v) NaCl. MK-7 was the sole respiratory quinone and the peptidoglycan type of 0W14T was A4βl-Orn-d-Glu. The major cellular fatty acid (>10.0 %) in strain 0W14T was anteiso-C15 : 0. The polar lipid profile of strain 0W14T consisted of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and four unknown phospholipids...
September 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28893359/crocinitomix-algicola-sp-nov-isolated-from-gracilaria-blodgettii
#10
Ming-Jing Shi, Ji-Ru Han, Hui Zhang, Zhi-Hong Xie, Zong-Jun Du
A Gram-stain-negative, strictly aerobic, non-flagellated, yellow-pigmented, rod-shaped bacterial strain, designated 0182T, was isolated from Gracilaria blodgettii, an algae of phylum Rhodophyta collected from coast of Lingshui county, Hainan, China (110° 03' 44.2'' E, 18° 24' 29.8'' N). The strain grew optimally at 30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. Cells of strain 0182T were approximately 0.9-2.5 µm in length and 0.2-0.4 µm in width. The major respiratory quinone was MK-7...
September 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28884672/geofilum-rhodophaeum-sp-nov-isolated-from-coastal-sediment
#11
Xin Mu, Zhen-Xing Xu, Da-Shuai Mu, Guan-Jun Chen, Zong-Jun Du
A novel Gram-stain-negative, facultatively anaerobic, filamentous and rose-brown pigmented bacterium, designated strain HF401T, was isolated from marine sediment off the coast of Weihai, China. The isolate grew at temperatures between 4 and 45 °C (optimal growth at 33 °C), at pH 6.5-8.5 (optimal growth at pH 7.5) and with 0.5-6.0 % (w/v) NaCl (optimal growth at 3.0 %). The predominant menaquinone was menaquinone 7 (MK-7). The G+C content of the genomic DNA was 50.8 mol% (from high-performance liquid chromatography)...
September 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28884658/sphingobacterium-corticis-sp-nov-isolated-from-bark-of-populus-%C3%A3-euramericana
#12
Yong Li, Guan-Tang Xu, Ju-Pu Chang, Li-Min Guo, Xu-Qi Yang, Chun-Gen Piao
A Gram-stain negative, aerobic, non-motile bacterial strain, 23D10-4-9T, was isolated from symptomatic canker bark tissue of Populus × euramericana. The isolate grew between 4 and 35 °C, with optimal growth occurring at 25 °C. The species was positive for catalase and negative for oxidase activity. Nitrate was not reduced to nitrite. It showed activities toward β-galactosidase and β-glucosidase. Citrate was not utilized. Acid was produced from d-glucose. The major fatty acids were iso-C15 : 0, C16 : 1ω7c and iso-C17 : 0 3-OH...
September 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28879837/bacillus-endozanthoxylicus-sp-nov-an-endophytic-bacterium-isolated-from-zanthoxylum-bungeanum-maxim-leaves
#13
Li Ma, Jia-Qin Xi, Yong-Hong Cao, Xiao-Yan Wang, Shuai-Chao Zheng, Cheng-Gang Yang, Ling-Ling Yang, Qi-Li Mi, Xue-Mei Li, Ming-Liang Zhu, Ming-He Mo
A Gram-stain-positive, rod-shaped, motile bacterium, designated as 1404T, was isolated from leaves of Chinese red pepper (Huajiao) (Zanthoxylum bungeanum Maxim) collected from Gansu, north-west China. Spores were not observed under a range of conditions. Strain 1404T was observed to grow at 15-45 °C and pH 6.0-10.0 and in presence of 0-5 % (w/v) NaCl concentration. The cell wall of strain 1404T was found to contain meso-diaminopimelic acid, and the predominant respiratory quinone was identified as MK-7...
September 7, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28875904/lewinella-maritima-sp-nov-and-lewinella-lacunae-sp-nov-novel-bacteria-from-marine-environments
#14
Heeyoung Kang, Haneul Kim, Yochan Joung, Kiseong Joh
Two Gram-staining-negative, rod-shaped, aerobic, marine bacteria, designated HME9321T and HME9359T, were isolated from seawater and lagoon water samples in the Republic of Korea. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains revealed that they belonged to the genus Lewinella within the family Saprospiraceae. The 16S rRNA gene sequence similarity of strain HME9321T showed highest similarities with Lewinella aquimaris HDW-36T (95.2 %), Lewinella marina MKG-38T (94.7 %) and Lewinella xylanilytica 13-9-B8T (94...
September 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28875897/chitinophaga-rhizosphaerae-sp-nov-isolated-from-rhizosphere-soil-of-a-tomato-plant
#15
Soo-Jin Kim, Hayoung Cho, Jae-Hyung Ahn, Hang-Yeon Weon, Jae-Ho Joa, Seung-Beom Hong, Soon-Ja Seok, Jeong-Seon Kim, Soon-Wo Kwon
An aerobic, Gram-stain-negative, non-spore-forming, non-flagellated, rod-shaped or filamentous bacterial strain, T16R-86T, was isolated from rhizosphere of a tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. It grew at the temperature range 10-37 °C (optimum, 28 °C) and pH range 6.0-9.0 (optimum, pH 7.0), and tolerated up to 2 % (w/v) NaCl. According to 16S rRNA gene sequence analysis, strain T16R-86T shared the highest similarity with Chitinophaga barathri YLT18T (96...
September 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28869058/enrichment-and-physiological-characterization-of-an-anaerobic-ammonium-oxidizing-bacterium-candidatus-brocadia-sapporoensis
#16
Yuko Narita, Lei Zhang, Zen-Ichiro Kimura, Muhammad Ali, Takao Fujii, Satoshi Okabe
We successfully enriched a novel anaerobic ammonium-oxidizing (anammox) bacterium affiliated with the genus 'Candidatus Brocadia' with high purity (>90%) in a membrane bioreactor (MBR). The enriched bacterium was distantly related to the hitherto characterized 'Ca. Brocadia fulgida' and 'Ca. Brocadia sinica' with 96% and 93% of 16S ribosomal RNA gene sequence identity, respectively. The bacterium exhibited the common structural features of anammox bacteria and produced hydrazine in the presence of hydroxylamine under anoxic conditions...
August 18, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28868998/conexibacter-stalactiti-sp-nov-isolated-from-stalactites-in-a-lava-cave-and-emended-description-of-the-genus-conexibacter
#17
Soon Dong Lee
A Gram-positive, strictly aerobic, non-sporulating, motile, rod-shaped bacterium, designated YC2-25T, was isolated from pieces of stalactites collected from a lava cave in Jeju, Republic of Korea. Colonies were circular, smooth, convex and cream to light yellow in colour. A neighbour-joining tree based on 16S rRNA gene sequences indicated that the organism belonged to the genus Conexibacter with the closest relatives being Conexibacter woesei (99.0 % sequence similarity) and Conexibacter arvalis (98.8 %)...
September 4, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28866991/bacillus-iocasae-sp-nov-isolated-from-pacmanus-hydrothermal-field-manus-basin
#18
Hai-Liang Wang, Jian Zhang, Li Sun
A novel bacterial strain S36T was isolated from the deep-sea sediment collected from Pacmanus hydrothermal field, Manus Basin. The strain was Gram-stain-positive, aerobic, rod-shaped, endospore-forming, and motile. It was able to grow at 16-50 °C, pH 6.0-10.0, and in the presence of 0-11 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain S36T was a member of genus Bacillus and shares the highest sequence identity with Bacillus herbersteinensis D-1,5aT (97.0 %). The value of DNA-DNA hybridization between strain S36T and B...
September 4, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28865073/spirosoma-daeguensis-sp-nov-isolated-from-beach-soil
#19
Nabil Elderiny, Leonid N Ten, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Young-Je Cho, Myung Kyum Kim, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-6(T), was isolated from beach soil on Jeju Island, South Korea. Strain 15J9-6(T), grew at 10-30°C (optimum growth at 25°C) and pH 7-8 (optimum growth at pH 7) on R2A, NA, and TSA agar. Phylogenetically, the strain was closely related to members of the genus Spirosoma (92.3-90.1% 16S rRNA gene sequence similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099(T) (92.3%). The G+C content of the genomic DNA of strain 15J9-6(T) was 45...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28857731/terrimonas-terrae-sp-nov-isolated-from-the-rhizosphere-of-a-tomato-plant
#20
Soo-Jin Kim, Hayoung Cho, Jae-Hyung Ahn, Hang-Yeon Weon, Jae-Ho Joa, Seung-Beom Hong, Soon-Ja Seok, Jeong-Seon Kim, Soon-Wo Kwon
A yellow, aerobic, Gram-stain-negative, non-motile, rod-shaped and non-flagellated bacterial strain, designated T16R-129T, was isolated from the rhizosphere of a tomato plant collected at a farm located on Buyeo-gun of Chungcheongnam-do, South Korea. Strain T16R-129T grew at 15-40 °C and pH 7.0-9.0, and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T16R-129T clustered with members of the genus Terrimonas, and it shared highest similarity with Terrimonas arctica R9-86T (96...
August 2017: International Journal of Systematic and Evolutionary Microbiology
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