keyword
https://read.qxmd.com/read/38443482/safety-evaluation-of-vitamin-k2-menaquinone-7-via-toxicological-tests
#1
JOURNAL ARTICLE
Sung-Bong Hwang, Min-Ju Choi, Hyeon-Jeong Lee, Jeong-Jun Han
This study aims to evaluate the safety of MK-7 produced by fermentation process using a Bacillus subtilis var. natto strain for human ingestion via acute oral toxicity, repeated dose 90-day oral toxicity, 28-day recovery test, and genotoxicity tests. The acute oral toxicity test results indicated that all subjects survived at the dose of 5000 mg/kg with no toxic effects. For the repeated dose 90-day oral toxicity test, MK-7 was administered to rats at 500, 1500, and 4500 mg/kg for 90 d. No abnormal findings were detected in clinical observations or in clinical pathological and histopathological examinations...
March 5, 2024: Scientific Reports
https://read.qxmd.com/read/38441685/xiashengella-succiniciproducens-gen-nov-sp-nov-a-succinate-producing-bacterium-isolated-from-an-anaerobic-digestion-tank-in-the-family-marinilabiliaceae-of-the-order-bacteroidales
#2
JOURNAL ARTICLE
Hao-Jie Huang, Xi Zhang, Xin-Wei Sun, Biao Chen, Xiu-Tong Li, Nan Zhou, Rashidin Abdugheni, Qiu-Yin Cheng, Tie-Jun Zhang, Yao Liu, Yong Jiang, Ye Deng, Shuang-Jiang Liu, Cheng-Ying Jiang
A strictly anaerobic, motile bacterium, designated as strain Ai-910T , was isolated from the sludge of an anaerobic digestion tank in China. Cells were Gram-stain-negative rods. Optimal growth was observed at 38 °C (growth range 25-42 °C), pH 8.5 (growth range 5.5-10.5), and under a NaCl concentration of 0.06% (w/v) (range 0-2.0%). Major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0 . The respiratory quinone was MK-7. Using xylose as the growth substrate, succinate was produced as the fermentation product...
March 5, 2024: Archives of Microbiology
https://read.qxmd.com/read/38429402/solibacillus-ferritrahens-sp-nov-a-novel-siderophore-producing-bacterium-isolated-from-wumeng-mountain-national-nature-reserve-in-yunnan-province
#3
JOURNAL ARTICLE
Xiao-Di Liu, Jiang-Yuan Zhao, Le-Le Li, Jian-Yu Li, Pei-Wen Yang, Song-Guo Liang, Lu-Yao Feng, Zhu-Feng Shi, Zhang-Gui Ding, Ming-Gang Li, Shu-Kun Tang
A gram-stain-positive, aerobic, rod-shaped bacterial strain capable of producing siderophores, named YIM B08730T , was isolated from a soil sample collected from Wumeng Mountain National Nature Reserve, Zhaotong City, Yunnan Province. Growth occurred at 10-45 °C (optimum, 35-40 ℃), pH 7.0-9.0 (optimum, 7.0) and in the presence of 0-5 % (w/v) NaCl (optimum, 0-1 %, w/v). A comparative analysis of the 16S rRNA gene sequence (1558 bp) of strain YIM B08730T showed the highest similarity to Solibacillus isronensis JCM 13838T (96...
March 1, 2024: Antonie Van Leeuwenhoek
https://read.qxmd.com/read/38369673/nesterenkonia-marinintestina-sp-nov-isolated-from-the-fish-intestine
#4
JOURNAL ARTICLE
Aolin Zhao, Hanqin Cai, Ying Huang, Quan Yang, Zhenxin Zhu, Yan Zhou, Mingguo Jiang, Yi Jiang, Wenshan Huang
A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115T , was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0-3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4-45 °C (optimum 30 °C), at pH 4.5-10.0 (optimum pH 7...
February 19, 2024: Archives of Microbiology
https://read.qxmd.com/read/38346457/comparative-genomics-analysis-and-transposon-mutagenesis-provides-new-insights-into-high-menaquinone-7-biosynthetic-potential-of-bacillus-subtilis-natto
#5
JOURNAL ARTICLE
Chunxu Jiang, Genhai Zhao, Han Wang, Wenqian Zheng, Rui Zhang, Li Wang, Zhiming Zheng
This research combined Whole-Genome sequencing, intraspecific comparative genomics and transposon mutagenesis to investigate the menaquinone-7 (MK-7) synthesis potential in Bacillus subtilis natto. First, Whole-Genome sequencing showed that Bacillus subtilis natto BN-P15-11-1 contains one single circular chromosome in size of 3,982,436 bp with a GC content of 43.85 %, harboring 4,053 predicted coding genes. Next, the comparative genomics analysis among strain BN-P15-11-1 with model Bacillus subtilis 168 and four typical Bacillus subtilis natto strains proves that the closer evolutionary relationship Bacillus subtilis natto BN-P15-11-1 and Bacillus subtilis 168 both exhibit strong biosynthetic potential...
February 10, 2024: Gene
https://read.qxmd.com/read/38344988/dual-sgrna-crispra-system-for-enhanced-mk-7-production-and-salmonella-infection-mitigation-in-bacillus-subtilis-natto-applied-to-caco-2-cells
#6
JOURNAL ARTICLE
Chaoyong Liao, Jian Cui, Mingkun Gao, Bo Wang, Koichi Ito, Yuming Guo, Bingkun Zhang
This study optimized the menaquinone-7 (MK-7) synthetic pathways in Bacillus subtilis ( B. subtilis ) natto NB205, a strain that originated from natto, to enhance its MK-7 production. Utilizing mutation breeding, we developed NBMK308, a mutant strain that demonstrated a significant 117.23% increase in MK-7 production. A comprehensive transcriptome analysis identified two key genes, ispA and ispE , as being critical in MK-7 synthesis. The dual-sgRNA CRISPRa system was utilized to achieve precise regulation of ispA and ispE in the newly engineered strain, A3E3...
February 12, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38334269/-paenibacillus-baimaensis-sp-nov-a-bacterium-isolated-from-mountain-soil-in-the-habitat-of-rhinopithecus-bieti
#7
JOURNAL ARTICLE
Qiong Wang, Xiu-Lin Han, Jian-Qiang Shen, Jian-Dong Lai, Chen-Lu Zhang, Zhi-Qin Fang, Tao Lu
A novel Gram-positive strain WQ 127069T that was isolated from the soil of Baima Snow Mountain, a habitat of highly endangered Yunnan snub-nosed monkeys ( Rhinopithecus bieti ), was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on the 16S rRNA gene sequences showed that the isolate belongs to the genus Paenibacillus , showing 98.4 and 96.08 % sequence similarity to the type strains Paenibacillus periandrae PM10T and Paenibacillus foliorum LMG 31456T , respectively. The G+C content of the genomic DNA of strain WQ127069T was 45...
February 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38329631/paenibacillus-lacisoli-sp-nov-a-mesotrione-degrading-strain-isolated-from-lakeside-soil
#8
JOURNAL ARTICLE
Qimiao Xu, Bin Liu, Yingying Wan, Xueting Jiang, Le Chen, Dongmei Mao, Gang Chen, Dan Cheng, Jian He, Qirong Shen
A Gram-stain-positive, facultatively anaerobic, rod-shaped bacterium, designated JX-17T , was isolated from a soil sample collected in Jiangxi Province, PR China. Growth was observed at 15-48 °C (optimum 37 °C), at pH 5.0-9.0 (optimum pH 7.0) and with 0-6.0% (w/v) NaCl (optimum 1.0%). Strain JX-17T could degrade approximately 50% of 50 mg/L mesotrione within 2 days of incubation, but could not use mesotrione as sole carbon source for growth. Strain JX-17T showed less than 95.3% 16S rRNA gene sequence similarity with type strains of the genus Paenibacillus...
February 8, 2024: Antonie Van Leeuwenhoek
https://read.qxmd.com/read/38323635/-solibacillus-palustris-sp-nov-isolated-from-wetland-soil-of-ecology-park
#9
JOURNAL ARTICLE
Jung-Hun Jo, Soon-Youl Lee, Moon-Soo Rhee, Kang-Hyun Lee, Se-Yoon Chun, Wan-Taek Im
A Gram-staining-positive, motile, aerobic and rod-shaped bacterium, designated strain MA9T was isolated from wetland soil of ecology park, in Seoul, Republic of Korea. This bacterium was characterized to determine its taxonomic position by using the polyphasic approach. Strain MA9T grew at 10-37 °C and at pH 6.0-9.5 on TSB. Menaquinone MK-7 was the predominant respiratory quinone and iso-C15 : 0 , iso-C16 : 0 and C16 : 1  ω7 c alcohol were the major fatty acids...
February 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38305710/-paenibacillus-glufosinatiresistens-sp-nov-a-glufosinate-resistant-bacterium-isolated-from-sludge
#10
JOURNAL ARTICLE
Yan Wu, Yuwen Yang, Minglai Wan, Le Chen, Ye Yuan, Jian He, Baolong Zhang
A Gram-stain-positive bacterium capable of resisting 5.0 mM glufosinate, designated strain YX-27T , was isolated from a sludge sample collected from a factory in Wuxi, Jiangsu, PR China. Cells were rod-shaped, facultatively anaerobic, endospore-forming, and motile by peritrichous flagella. Growth was observed at 15-42 °C (optimum at 30 °C), pH 4.0-8.0 (optimum pH 7.0-7.5) and with 0-2.5% NaCl (w/v; optimum, 0.5 %). Strain YX-27T could tolerate up to 6.0 mM glufosinate...
February 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38194140/adaptive-laboratory-evolution-for-improved-tolerance-of-vitamin-k-in-bacillus-subtilis
#11
JOURNAL ARTICLE
Xiumin Ding, Zhiming Zheng, Genhai Zhao, Li Wang, Han Wang, Peng Wang
Menaquinone-7 (MK-7), a subtype of vitamin K2 (VK2 ), assumes crucial roles in coagulation function, calcium homeostasis, and respiratory chain transmission. The production of MK-7 via microbial fermentation boasts mild technological conditions and high biocompatibility. Nevertheless, the redox activity of MK-7 imposes constraints on its excessive accumulation in microorganisms. To address this predicament, an adaptive laboratory evolution (ALE) protocol was implemented in Bacillus subtilis BS011, utilizing vitamin K3 (VK3 ) as a structural analog of MK-7...
December 2024: Applied Microbiology and Biotechnology
https://read.qxmd.com/read/38189996/description-and-genomic-characterization-of-cohnella-caldifontis-sp-nov-isolated-from-hot-springs-in-yunnan-province-south-west-china
#12
JOURNAL ARTICLE
Ming-Xian Xiang, Cui-Ping Miao, Dian-Yan Zhang, Juan Wang, Yi-Qing Li, Min Yin, ShuKun Tang
A bacterial strain, Gram staining positive, strictly aerobic, rod-shaped, motile bacterium with flagellum and endospore-forming, designated strain YIM B05605T , was isolated from soil sampled in Hamazui hot springs, Tengchong City, Yunnan province, China. Optimum growth for the strain occurred at pH 7.0 and 45 °C. MK-7 was the main menaquinone in the strain YIM B05605T . The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), unidentified glycolipid (GL), three unknown aminophospholipids (APLs) and unidentified polarlipid (PL) were part of the polar lipid profile...
January 8, 2024: Antonie Van Leeuwenhoek
https://read.qxmd.com/read/38180019/-shewanella-goraebulensis-sp-nov-isolated-from-sea-water
#13
JOURNAL ARTICLE
Yong-Suk Lee, Hae-Rin Jeong, Eun-Jung Hwang, Maheshkumar Prakash Patil, Yong Bae Seo, Gun-Do Kim
A novel Gram-stain-negative, facultatively anaerobic and rod-shaped bacterial strain, designated as DAU312T , was isolated from the sea water of the eastern coast of the Republic of Korea. Optimal growth was observed at 25 °C, pH 7.0-8.0 and with NaCl concentrations of 2.0 % (w/v). Catalase and oxidase activities were detected. On the basis of 16S rRNA gene sequences, strain DAU312T showed the highest similarity (99.2 %) to the type strain Shewanella electrodiphila MAR441T . The complete genome sequence of strain DAU312T contains 4 893 483 bp and 40...
January 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38172964/revisiting-the-interconnection-between-lipids-and-vitamin-k-metabolism-insights-from-recent-research-and-potential-therapeutic-implications-a-review
#14
REVIEW
Jing Tan, Ying Li
Vitamin K is a lipophilic vitamin, whose absorption, transportation, and distribution are influenced by lipids. The plasma vitamin K level after supplementation is predominantly a lipid-driven effect and independent of existing vitamin K status. However, previous studies examining the efficacy of vitamin K supplementation often overlooked the influence of lipid levels on vitamin K absorption, resulting in inconsistent outcomes. Recent research discovered that impaired transportation of vitamin K2 within uremic high-density lipoproteins (HDL) in individuals with uremia might elucidate the lack of beneficial effects in preventing calcification observed in multiple trials involving menaquinone-7 (MK-7) supplementation among patients with chronic kidney disease...
January 3, 2024: Nutrition & Metabolism
https://read.qxmd.com/read/38170331/description-of-nesterenkonia-aerolata-sp-nov-an-actinobacterium-isolated-from-air-of-manufacturing-shop-in-a-pharmaceutical-factory
#15
JOURNAL ARTICLE
Ming-Hui Song, Wen-Long Ma, Ning Zhang, Wei He, Hong-Chuan Wang, Shuang Wang, Yi-Ling Fan, Dao-Feng Zhang
During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111T , was isolated from a settling dish. Strain LY-0111T grew at temperature of 10-42 °C (optimum 35 °C), pH of 5.0-10.0 (optimum pH 7.0) and NaCl concentration of 1-12% (optimum 0.5-3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to Nesterenkonia halophila YIM 70179T (96...
January 3, 2024: Antonie Van Leeuwenhoek
https://read.qxmd.com/read/38160217/aureispira-anguillae-sp-nov-isolated-from-japanese-eel-anguilla-japonica-leptocephali
#16
JOURNAL ARTICLE
Kei Yuasa, Tohru Mekata, Ikunari Kiryu, Kazuharu Nomura, Ryusuke Sudo, Masataka Satomi
A novel filamentous eel-leptocephalus pathogenic marine bacterium, designated strain EL160426T , was isolated from Japanese eel, Anguilla japonica, leptocephali reared at a laboratory in Mie, Japan. In experimental infection studies on eel larvae, the strain EL160426T caused massive larval mortality and was reisolated from moribund leptocephali. Characteristically, observations of infected larvae found that EL160426T forms columnar colonies on the cranial surface of larvae. The novel isolate exhibited growth at 15-30 °C, pH 7-9, and seawater concentrations of 60-150% (W/V)...
December 30, 2023: Archives of Microbiology
https://read.qxmd.com/read/38158723/vitamin-d-and-vitamin-k-status-in-postmenopausal-women-with-normal-and-low-bone-mineral-density
#17
JOURNAL ARTICLE
Sieglinde Zelzer, Andreas Meinitzer, Dietmar Enko, Konstantinos Markis, Symeon Tournis, Ioulia Trifonidi, Efstathios Chronopoulos, Loukia Spanou, Nerea Alonso, Martin Keppel, Markus Herrmann
OBJECTIVES: Vitamin D and K are believed to promote bone health, but existing evidence is controversial. This study aimed to measure several metabolites of both vitamins by liquid chromatography tandem mass spectrometry (LC-MS/MS) in a cohort of postmenopausal women with low and normal bone mineral density (BMD). METHODS: Vitamin metabolites (25-hydroxyvitamin D (25[OH]D), 24,25-dihydroxyvitamin D (24,25(OH)2 D), phylloquinone (K1), menaquinone-4 (MK-4) and MK-7) were measured in 131 serum samples by LC-MS/MS...
January 1, 2024: Clinical Chemistry and Laboratory Medicine: CCLM
https://read.qxmd.com/read/38153563/enhancing-menaquinone-7-biosynthesis-through-strengthening-precursor-supply-and-product-secretion
#18
JOURNAL ARTICLE
Pengchen Hu, Cheng Peng, Bei Zhang, Xuechao Hu, Ripon Baroi Milon, Lujing Ren
Menaquinone-7 (MK-7) is an important class of vitamin K2 that is essential in human health and can prevent osteoporosis and cardiovascular disease. However, due to the complex synthesis pathway, the synthesis efficiency is low. The main objective of this study was to explore the effect of enhanced supply of precursors in Bacillus natto. Three precursors of pyruvate, shikimic acid, and sodium glutamate were chosen to investigate the effect of enhanced supply of precursors on MK-7 synthesis. Then, the optimal concentrations, different combinations, and different adding times were systematically studied, respectively...
December 28, 2023: Bioprocess and Biosystems Engineering
https://read.qxmd.com/read/38148391/micrococcus-lacusdianchii-sp-nov-an-attached-bacterium-inhibited-by-metabolites-from-its-symbiotic-algae
#19
JOURNAL ARTICLE
Le Wang, Yao Xiao, Wenxin Lai, Ru Jia, Qinglin Deng, Xin Wang, Hongqiu Shi, Yiwen Yang, Binghuo Zhang
A novel actinobacterial strain, designated as JXJ CY 30 T , was isolated from the phycosphere of Microcystis aeruginosa FACHB-905 (Maf) collected from Lake Dianchi, China. The strain was a Gram-stain-positive, aerobic and coccus-shaped actinobacterium. It had alanine, glutamic acid, aspartic acid, and lysine in the peptidoglycan, and mannose, ribose and arabinose in its cell wall sugars, anteiso-C15:0 and iso-C15:0 as the main cellular fatty acids, MK-7 and MK-8 as the major respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, glycolipid, and an unidentified phospholipid as the polar lipids...
December 26, 2023: Journal of Antibiotics
https://read.qxmd.com/read/38147140/paenibacillus-silvisoli-sp-nov-and-paenibacillus-humicola-sp-nov-isolated-from-forest-soil
#20
JOURNAL ARTICLE
Hyosun Lee, Dhiraj Kumar Chaudhary, Oung Bin Lim, Dong-Uk Kim
During the study of microbial ecology of forest soil, two circular, white-colored bacterial colonies were isolated and labeled as strains TW38T and TW40T . Both strains were catalase positive and oxidase negative. Strains TW38T and TW40T demonstrated growth within a temperature range of 10-37 °C and 18-37 °C, respectively, and thrived within a pH range of 5.5-9.0 and 6.0-8.0, respectively. Both strains grew at 0-2.0% (w/v) NaCl concentrations. The phylogenetic analysis indicated that strains TW38T and TW40T affiliated to the genus Paenibacillus, with the closest neighbors being Paenibacillus montanisoli RA17T (98...
December 26, 2023: Archives of Microbiology
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