Xuehan Zhou, Carrie Stine, Patricia Oliveira Prada, Debora Fusca, Kevin Assoumou, Jan Dernic, Musadiq A Bhat, Ananya S Achanta, Joseph C Johnson, Sanjana Jadhav, Corinna A Bauder, Lukas Steuernagel, Luca Ravotto, Dietmar Benke, Bruno Weber, Miriam Stoeber, Peter Kloppenburg, Jens C Brüning, Michael R Bruchas, Tommaso Patriarchi
Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release...
June 1, 2023: bioRxiv