Chanelle L Acheamfour, Salina Parveen, Alan Gutierrez, Eric T Handy, Sara Behal, Donghyun Kim, Seongyun Kim, Cheryl East, Ray Xiong, Joseph R Haymaker, Shirley A Micallef, Rachel E Rosenberg Goldstein, Kalmia E Kniel, Amy R Sapkota, Fawzy Hashem, Manan Sharma
Alternative irrigation waters (rivers, ponds, and reclaimed water) can harbor bacterial foodborne pathogens like Salmonella enterica and Listeria monocytogenes , potentially contaminating fruit and vegetable commodities. Detecting foodborne pathogens using qPCR-based methods may accelerate testing methods and procedures compared to culture-based methods. This study compared detection of S. enterica and L. monocytogenes by qPCR (real-time PCR) and culture methods in irrigation waters to determine the influence of water type (river, pond, and reclaimed water), season (winter, spring, summer, and fall), or volume (0...
February 20, 2024: Microbiology Spectrum