Catalina I Pislariu, Jeremy D Murray, JiangQi Wen, Viviane Cosson, RajaSekhara Reddy Duvvuru Muni, Mingyi Wang, Vagner A Benedito, Andry Andriankaja, Xiaofei Cheng, Ivone Torres Jerez, Samuel Mondy, Shulan Zhang, Mark E Taylor, Million Tadege, Pascal Ratet, Kirankumar S Mysore, Rujin Chen, Michael K Udvardi
A Tnt1-insertion mutant population of Medicago truncatula ecotype R108 was screened for defects in nodulation and symbiotic nitrogen fixation. Primary screening of 9,300 mutant lines yielded 317 lines with putative defects in nodule development and/or nitrogen fixation. Of these, 230 lines were rescreened, and 156 lines were confirmed with defective symbiotic nitrogen fixation. Mutants were sorted into six distinct phenotypic categories: 72 nonnodulating mutants (Nod-), 51 mutants with totally ineffective nodules (Nod+ Fix-), 17 mutants with partially ineffective nodules (Nod+ Fix+/-), 27 mutants defective in nodule emergence, elongation, and nitrogen fixation (Nod+/- Fix-), one mutant with delayed and reduced nodulation but effective in nitrogen fixation (dNod+/- Fix+), and 11 supernodulating mutants (Nod++Fix+/-)...
August 2012: Plant Physiology