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https://www.readbyqxmd.com/read/28429088/an-snp-panel-for-the-analysis-of-paternally-inherited-alleles-in-maternal-plasma-using-ion-torrent-pgm
#1
Donggui Yang, Hao Liang, Shaobin Lin, Qing Li, Xiaoyan Ma, Jun Gao, Hongyu Sun, Qingqing Chen, Jianzhu Wu, Xueling Ou
Researchers have sought to develop an effective protocol for paternity analysis using cell-free DNA (cfDNA) in maternal plasma. The use of massively parallel sequencing (MPS) technology for SNP testing is attractive because of its high-throughput capacity and resolution to single-base precision. In this study, we designed a customized SNP panel for cfDNA sequencing that includes 720 short amplicons (< 140 bp) targeting SNPs on the autosome and Y chromosome. The systemic performance was evaluated using the Ion Torrent PGM, indicating balanced coverage among most of the included loci, except for 78 poorly performing SNPs that were observed to have an inconsistent allele balance, lower coverage reads or high background signals...
April 20, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28428000/selected-oxidative-stress-biomarkers-in-antenatal-diagnosis-as-11-14-gestational-weeks
#2
Marek Pietryga, Piotr Dydowicz, Kinga Toboła, Marta Napierała, Izabela Miechowicz, Anna Gąsiorowska, Maciej Brązert, Ewa Florek
The primary objective in modern obstetrics and prenatal diagnosis is to predict risks of congenital abnormalities. The aim of the research was to assess the correlation between selected oxidative stress biomarkers with the risk of foetal chromosomal aberration evaluated at the first trimester screening. A series of studies show that balanced free radical activity and oxidative homeostasis are essential for proper bodily growth and function. Reactive oxygen species (ROS) may be one of the factors associated with disruption of cell cycle and tissue development, thus leading to developmental abnormalities...
April 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28424869/review-on-diagnosis-and-management-of-urolithiasis-in-pregnancy-an-esut-practical-guide-for-urologists
#3
REVIEW
Bhaskar K Somani, Athanasios Dellis, Evangellos Liatsikos, Andreas Skolarikos
PURPOSE: Management of urolithiasis in pregnancy can be challenging for most urologists with diagnostic and treatment dilemma to ensure the best outcome for both mother and fetus. We wanted to review the literature for urolithiasis in pregnancy with a practical management guide for urologists. METHODS: A non-systematic review of literature was carried out for all English language literature using Medline. To ensure a more comprehensive search, the review of diagnosis and management of pregnant patients with urolithiasis was carried out separately, by two authors independently...
April 19, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/28411128/the-effects-of-experimental-gestational-hypertension-on-maternal-blood-pressure-and-fluid-intake-and-pre-weanling-hypothalamic-neuronal-activity
#4
Juan Arguelles, Carmen Perillan, Terry G Beltz, Baojian Xue, Daniel Badaue-Passos, Jose A Vega, Alan Kim Johnson
To examine the fetal programming effects of maternal hypertension, natriophilia and hyperreninemia [experimentally induced in rats by partial inter-renal aortic ligature (PAL) prior to mating] fos immunoreactivity was studied in 6-day-old offspring of PAL and control mothers. The purposes of the present set of experiments were twofold. The first was to characterize the effects of PAL on the mother's arterial blood pressure and intake of salt (1.8% NaCl solution) and water over the course of gestation. Second, was to study the pattern of neuronal activation in key brain areas of 6-day-old offspring treated with the dipsogen isoproterenol that were from PAL and control mothers...
April 11, 2017: Appetite
https://www.readbyqxmd.com/read/28410127/oxidative-stress-major-executioner-in-disease-pathology-role-in-sperm-dna-damage-and-preventive-strategies
#5
Shilpa Bisht, Rima Dada
Oxidative stress (OS) has been implicated in a wide array of diseases such as neurodegenerative disorders, autoimmune diseases, complex lifestyle diseases and cancer. OS is caused by an imbalance in production of Reactive Oxygen Species (ROS) and antioxidant defenses in the cell which results in the damage of cellular components, inactivate essential metabolic enzymes and disrupt signal transduction pathways. OS induces peroxidative damage to the sperm plasma membrane, DNA fragmentation in sperm nuclear/mitochondrial genome and causes dysregulation in levels of mRNAs/transcripts...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28404581/maternal-and-postnatal-high-fat-diet-consumption-programs-energy-balance-and-hypothalamic-melanocortin-signaling-in-nonhuman-primate-offspring
#6
Elinor L Sullivan, Heidi M Rivera, Cadence A True, Juliana G Franco, Karalee Baquero, Tyler A Dean, Jeanette C Valleau, Diana L Takahashi, Tim Frazee, Genevieve Hanna, Melissa A Kirigiti, Leigh Ann A Bauman, Kevin L Grove, Paul Kievit
Maternal high-fat diet (HFD) consumption during pregnancy decreased fetal body weight and impacted development of hypothalamic melanocortin neural circuitry in nonhuman primate offspring. We investigated whether these impairments during gestation persisted in juvenile offspring and examined the interaction between maternal and early postnatal HFD consumption. Adult dams consumed either a control diet (CTR, 15% calories from fat) or a high saturated-fat diet (HFD, 37% calories from fat) during pregnancy. Offspring were weaned onto a CTR or HFD at approximately 8 months of age...
April 12, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28395333/ovine-uterine-space-restriction-causes-dysregulation-of-the-renin-angiotensin-system-in-fetal-kidneys%C3%A2
#7
Rachel A Kranch-Shorthouse, Adam S Bauer, Ronald R Magness, Gladys E Lopez, Jeffrey L Segar, Sharon E Blohowiak, Pamela J Kling
In ovine pregnancy, uterine space restriction (USR) resulting from decreased space for placental attachment caused intrauterine growth restriction and impaired nephrogenesis. The fetal kidney renin-angiotensin system (RAS) is involved in nephrogenesis, fluid balance, and iron deposition. Angiotensin II exerts its effects via multiple receptors: angiotensin II 1-8 receptor type 1 (AT 1 R) and type 2 (AT 2 R), and angiotensin II 1-7 Mas receptor (MASR). Objective: : To test the hypothesis that ovine USR is associated with dysregulation of the fetal renal RAS...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28390563/biomarkers-for-adverse-pregnancy-outcomes-in-rheumatic-diseases
#8
REVIEW
May Ching Soh, Catherine Nelson-Piercy
Pregnancy is a delicate balance of angiogenic factors. Adverse pregnancy outcomes in the form of placental insufficiency occur when antiangiogenic factors predominate, which manifests as maternal-placental syndrome (MPS). Women with rheumatic disease are at increased risk of MPS. Endothelial damage from circulating antiangiogenic factors and other inflammatory molecules in combination with preexisting maternal vascular risk factors is the likely underlying pathophysiological process for MPS. It is likely that these changes persist, and additional "insults" from ongoing inflammation, medications, and disease damage contribute to the development of accelerated cardiovascular disease seen in young women with rheumatic disease...
May 2017: Rheumatic Diseases Clinics of North America
https://www.readbyqxmd.com/read/28389753/the-role-of-hsp70-in-the-regulation-of-autophagy-in-gametogenesis-pregnancy-and-parturition
#9
Steven S Witkin, Tomi T Kanninen, Giovanni Sisti
Induction of the 70 kDa heat shock protein (hsp70) and autophagy are two major mechanisms that promote cell homeostasis during the rapid cell growth and differentiation characteristic of reproduction. Hsp70 insures proper assembly, conformation, and intracellular transport of nascent proteins. Autophagy removes from the cytoplasm proteins, other macromolecules, and organelles that are no longer functional or needed and recycles their components for synthesis of new products under nutritionally limiting conditions...
2017: Advances in Anatomy, Embryology, and Cell Biology
https://www.readbyqxmd.com/read/28389056/effects-of-adipokines-and-obesity-on-uterine-contractility
#10
REVIEW
Henri Azaïs, Audrey Leroy, Louise Ghesquiere, Philippe Deruelle, Sandy Hanssens
Obesity is a major public health problem. The prevalence of obesity has significantly increased in developed countries, particularly in France with an overall increase of 76% over the last 15 years. In pregnant women, obesity is associated with alterations in the quality of labor, such as delayed onset of labor, a higher rate of prolonged pregnancies, prolonged labor, and higher oxytocin requirements. There is also an increased prevalence of Cesarean sections, particularly during the active phase of labor, and perinatal complications (postpartum hemorrhage)...
January 13, 2017: Cytokine & Growth Factor Reviews
https://www.readbyqxmd.com/read/28387884/the-probiotics-in-dentistry-a-narrative-review
#11
A M Pujia, M Costacurta, L Fortunato, G Merra, S Cascapera, M Calvani, S Gratteri
The total number of microbes that colonize the human body is far greater than the number of cells that make it up. In recent years, it has been shown that bacteria play an essential role in the body; in fact, they are essential for the maturation of the intestine, the development and control of the immune system, the development of the brain, the metabolism of macronutrients, the synthesis of vitamins, and the energy balance. Bacteria play an essential role in defense of their territory against the entry of other bacteria that may be pathogenic to health...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28385397/maternal-age-modulates-the-effects-of-early-pregnancy-l-proline-supplementation-on-the-birth-weight-of-piglets
#12
P Gonzalez-Añover, A Gonzalez-Bulnes
Previous results obtained in gilts maintained under experimental conditions suggest that amino acid supplementation during pregnancy may be a promising strategy for diminishing the incidence of embryo losses and low birth-weight newborn. The current study evaluated the effects of a short-term supplementation with L-proline, around implantational stages, on litter size and birth-weight of piglets in sows of different parities maintained under commercial farm conditions. There were no significant effects in mature sows with three or more parities, but the supplementation improved the reproductive efficiency of the high-prolific first-parity sows and of all the sows at second-parity...
March 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28385340/lacosamide-during-pregnancy-and-breastfeeding
#13
Simona Lattanzi, Claudia Cagnetti, Nicoletta Foschi, Leandro Provinciali, Mauro Silvestrini
BACKGROUND: The epilepsy treatment during pregnancy represents a balance between teratogenic hazard and seizure control. The aim of the study was to evaluate the safety and efficacy of lacosamide (LCS) during pregnancy and breastfeeding. METHODS: Patients referred to our Epilepsy Center for pregnancy planning who became pregnant while taking LCS were prospectively followed-up. Data on seizure frequency, side effects, pregnancy course, delivery and breastfeeding, birth outcome, congenital malformation and development of newborns were collected...
March 30, 2017: Neurologia i Neurochirurgia Polska
https://www.readbyqxmd.com/read/28381873/from-apelin-to-exercise-emerging-therapies-for-management-of-hypertension-in-pregnancy
#14
Jeffrey S Gilbert
Studies over the last couple of decades have provided exciting new insights into mechanisms underlying the pathogenesis of preeclampsia. In addition, several novel and innovative molecules and ideas for management of the syndrome have also come forth. While our basic understanding of the initiating events of preeclampsia continues to be placental ischemia/hypoxia stimulating the release of a variety of factors from the placenta that act on the cardiovascular and renal systems, the number of candidate pathways for intervention continues to increase...
April 6, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28380605/crude-glycerin-decreases-nonesterified-fatty-acid-concentration-in-ewes-during-late-gestation-and-early-lactation
#15
D M Polizel, I Susin, R S Gentil, E M Ferreira, R A de Souza, A P A Freire, A V Pires, M V C Ferraz, P H M Rodrigues, M L Eastridge
Crude glycerin is a gluconeogenic substrate in ruminants and may help to decrease the occurrence of pregnancy toxemia. The objective in this trial was to determine the effects of feeding a diet containing crude glycerin on DMI, milk yield, milk composition, and blood metabolites in periparturient ewes and lamb performance. One hundred eighteen 90 (±1.1)-d pregnant Santa Inês ewes were used. After lambing, 32 ewes (62.8 ± 1.3 kg BW) were allotted in a randomized complete block design defined by prelambing diet, BW, BCS, lambing date, type of birth (single or twin), and sex of offspring...
February 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28379830/fertility-treatment-use-of-in-vitro-fertilization-and-time-to-live-birth-based-on-initial-provider-type
#16
Mandy W Boltz, Jessica N Sanders, Sara E Simonsen, Joseph B Stanford
PURPOSE: To explore the relationship between the type of clinician (generalist vs subspecialist) initially seen by infertile women, the treatment received, and the time to pregnancy. METHODS: We analyzed mixed-mode questionnaire data from 867 women with primary infertility enrolled into a retrospective cohort through population- and fertility clinic-based sampling. We compared women presenting first to generalist providers with women presenting first to fertility subspecialists, with the main outcomes of receiving in vitro fertilization (IVF), time to pregnancy, and live birth...
March 2017: Journal of the American Board of Family Medicine: JABFM
https://www.readbyqxmd.com/read/28378505/prolactin-receptors-in-rip-cre-cells-but-not-in-agrp-neurons-are-involved-in-energy-homeostasis
#17
S R Ladyman, M A MacLeod, Z Khant Aung, P Knowles, H R Phillipps, R S E Brown, D R Grattan
Among its many functions, prolactin has been implicated in energy homeostasis, particularly during pregnancy and lactation. The arcuate nucleus is a key site in the regulation of energy balance. The aim of this study was to examine whether arcuate nucleus neuronal populations involved in energy homeostasis are prolactin responsive and if they can mediate the effects of prolactin on energy homeostasis. To determine if Agrp neurons or Rip-Cre neurons are prolactin responsive, transgenic mice expressing the reporter td-tomato in Agrp neurons (td-tomato/AgRP-Cre) or Rip-Cre neurons (td-tomato/Rip-Cre) were treated with prolactin and perfused 45 minutes later...
April 4, 2017: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/28377131/bovine-herpesvirus-1-modified-live-virus-vaccines-for-cattle-reproduction-balancing-protection-with-undesired-effects
#18
Christopher C L Chase, Robert W Fulton, Donal O'Toole, Benjamin Gillette, Russell F Daly, George Perry, Travis Clement
Bovine herpesvirus 1 (BoHV-1) has long been associated with reproductive failure in cattle following infection of the ovary and/or fetus. Vaccination prior to breeding has been an effective approach to lessen the impact of BoHV-1 on reproduction. Prior studies in the 1980s and 1990s established the susceptibility of the ovary and particularly the corpus luteum (CL) to BoHV-1 infection. A series of studies at breeding time established that: (1) in naïve animals, the CL was the major target of BoHV-1 pathology; (2) CL lesions occurred within 4-9 days after estrus; (3) similar lesions was seen with BoHV-1 MLV vaccines; (4) ovarian lesions varied by the vaccine strain used; (5) progesterone decreased with or without CL lesions; and (6) following reactivation of BoHV-1 latent infection, ovaries could become reinfected in the face of BoHV-1 immunity...
March 23, 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28372936/-every-person-s-just-different-women-s-experiences-with-counseling-for-early-pregnancy-loss-management
#19
Robin Wallace, Angela DiLaura, Christine Dehlendorf
BACKGROUND: Women have strong preferences for their choice of early pregnancy loss (EPL) management. However, current practice patterns suggest that some women may not be offered counseling about the full range of management options. To develop a foundation for quality counseling about EPL treatment, we elicited patients' perspectives regarding their preferences and values for communication during this decision-making process. METHODS: Twenty-one individual interviews were conducted with women who recently experienced EPL...
March 31, 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
https://www.readbyqxmd.com/read/28368790/-should-physical-activity-be-contraindicated-during-pregnancy-in-relation-to-its-potentially-related-risks
#20
S Verdière, B C Guinhouya, D Salerno, P Deruelle
OBJECTIVE: This paper aimed at providing a critical analysis of data on the risks associated with physical activity of women during their pregnancy. METHODS: Cochrane Library, Google Scholar, PubMed (Medline) and Web of Sciences were searched using a combination of MeSH terms associated to "Physical activity", "Pregnancy" and "Adverse events" or "Risks". Only review papers published from inception of these databases to November 2016 were used in the present analysis...
February 2017: Gynecologie, Obstetrique, Fertilite & Senologie
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