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https://www.readbyqxmd.com/read/28212665/clinical-outcome-of-periodontal-regenerative-therapy-using-collagen-membrane-and-deproteinized-bovine-bone-mineral-a-2-5-year-follow-up-study
#1
Daisuke Irokawa, Takahiro Takeuchi, Katsuya Noda, Hiroaki Goto, Masahiro Egawa, Sachiyo Tomita, Hiroki Sugito, Masahiko Nikaido, Atsushi Saito
BACKGROUND: This study aimed to evaluate, longitudinally, the outcome of periodontal regenerative therapy using a deproteinized bovine bone mineral (DBBM) in combination with a collagen barrier (CB) for the treatment of intrabony defects. RESULTS: Patients with chronic periodontitis who have completed initial periodontal therapy participated in this study. They had at least one 2- or 3-wall intrabony periodontal defect of ≥3 mm in depth. During surgery, defects were filled with DBBM and covered with CB...
February 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28211197/recurrences-in-the-blanking-period-and-12-month-success-rate-by-continuous-cardiac-monitoring-after-cryoablation-of-paroxysmal-and-non-paroxysmal-atrial-fibrillation
#2
Paolo Pieragnoli, Alessandro Paoletti Perini, Giuseppe Ricciardi, Luca Checchi, Andrea Giomi, Iacopo Muraca, Letizia Mannucci, Luigi Padeletti
INTRODUCTION: Recurrences within the blanking period (early recurrences) are common after atrial fibrillation (AF) ablation by pulmonary vein isolation (PVI), but their clinical significance is still controversial. We aimed at evaluating the significance of within-blanking recurrences at 12-month follow-up after cryoballoon (CB) PVI, and to assess the real procedural success rate by continuous monitoring of cardiac rhythm. METHODS AND RESULTS: Sixty consecutive AF patients (34 paroxysmal, 56...
February 17, 2017: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/28210729/self-assembly-of-cucurbit-7-uril-based-triangular-4-molecular-necklaces-and-their-fluorescence-properties
#3
Soumen K Samanta, Kimberly G Brady, Lyle Isaacs
Self-assembly of rigid-rod dipyridine ligand 1 with M(en)(NO3)2 (M = Pd, Pt) affords triangular (3, 5) and square (4, 6) supramolecular coordination complexes (SCCs). The binding affinity of 1 toward CB[n]-type containers results in the formation of triangular [4]molecular necklaces ([4]MNs, 7-10) by either one-pot or post complexation approaches as evidenced by (1)H NMR, diffusion ordered spectroscopy, and ESI-MS.
February 17, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28208352/higher-order-effects-on-the-properties-of-the-optical-compact-bright-pulse-collective-variable-approach
#4
Chancelor Pokam Nguewawe, Serge I Fewo, David Yemélé
The effects of higher-order (HO) terms on the properties of the compact bright (CB) pulse described by the dispersionless nonlocal nonlinear Schrödinger (DNNLS) equation are investigated. These effects include third-order dispersion (TOD), the Raman term, and the time derivative of the pulse envelope. By means of the collective variable method, the dynamical behavior of the pulse amplitude, width, frequency, velocity, phase, and chirp during propagation is pointed out. The results indicate that the CB pulse experiences a self-frequency shift and self-steepening, respectively, in the presence of an isolated Raman term and the time derivative of the pulse envelope and acquires a velocity as the result of the TOD effect...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28205427/utility-of-brca1-associated-protein-1-immunoperoxidase-stain-to-differentiate-benign-versus-malignant-mesothelial-proliferations-in-cytologic-specimens
#5
Zulfia McCroskey, Gregg Staerkel, Sinchita Roy-Chowdhuri
BACKGROUND: Loss of BAP1 has recently been described as a highly specific marker for distinguishing malignant mesotheliomas (MM) from benign mesothelial proliferations (BMP). The aim of this study was to evaluate the utility of BAP1 in cytospin and cell block (CB) preparations. METHODS: We searched the database at our institution for cases of MM (n = 21) and BMP (n = 11). A Papanicolaou stained cytospin preparation and CB section from each case were selected for BAP1 staining...
February 16, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28198190/mining-2-2-complexes-from-1-1-stoichiometry-formation-of-cucurbit-8-uril-diarylviologen-quaternary-complexes-favored-by-electron-donating-substituents
#6
Guanglu Wu, Magdalena Olesińska, Yuchao Wu, Dijana Matak-Vinkovic, Oren A Scherman
A 1:1 binding stoichiometry of a host-guest complex need not consist of a single host and guest. Diarylviologens containing electron-donating substituents complexed with cucurbit[8]uril (CB[8]) in a 1:1 stoichiometry exhibit abnormally large binding enthalpies compared to typical enthalpy changes observed for 1:1 binary complexes. Here, several CB[8]-mediated host-guest complexes, which were previously reported as 1:1 binary complexes, are verified to be 2:2 quaternary complexes by a combination of isothermal titration calorimetry, (1)H, NOESY, and ROESY NMR, and ion mobility mass spectrometry, clearly indicating a binding motif of two partially overlapping diarylviologens held in place with two CB[8] molecules...
February 15, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28196863/conformational-changes-in-the-activation-loop-of-mitochondrial-glutaminase-c-a-direct-fluorescence-read-out-that-distinguishes-the-binding-of-allosteric-inhibitors-from-activators
#7
Clint A Stalnecker, Jon W Erickson, Richard A Cerione
The first step in glutamine catabolism is catalyzed by the mitochondrial enzyme glutaminase, with a specific isoform, glutaminase C (GAC), being highly expressed in cancer cells. GAC activation requires the formation of homo-tetramers, promoted by anionic allosteric activators such as inorganic phosphate. This leads to the proper orientation of a flexible loop proximal to the dimer-dimer interface that is essential for catalysis (i.e. the activation loop). A major class of allosteric inhibitors of GAC, with the prototype being BPTES (bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide), and the related molecule CB-839, binds to the activation loop and induces the formation of an inactive tetramer (2 inhibitors bound per active tetramer)...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28196153/periodontal-regenerative-therapy-of-intrabony-defects-using-deproteinized-bovine-bone-mineral-in-combination-with-collagen-barrier-membrane-a-multicenter-prospective-case-series-study
#8
Daisuke Irokawa, Nobuki Okubo, Masahiko Nikaido, Hiroyasu Shimizu, Nobuyuki Konobu, Tokuo Matsui, Takahisa Fujita, Hiroaki Goto, Takahiro Takeuchi, Yoshihito Ishii, Atsushi Saito
This multicenter prospective case series study aimed to evaluate the outcome of periodontal regenerative therapy using a deproteinized bovine bone mineral (DBBM) in combination with a collagen barrier (CB) in the treatment of intrabony defects. A total of 36 nonsmoking patients with chronic periodontitis were recruited in five centers in Japan. All patients had at least one intrabony defect of ≥ 3 mm. The surgical procedures included access for debridement using a papilla preservation technique. Defects were filled with DBBM and covered with CB...
February 14, 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28195371/assessment-of-cooperativity-in-ternary-peptide-cucurbit-8-uril-complexes
#9
Emanuela Cavatorta, Pascal Jonkheijm, Jurriaan Huskens
Evaluating cooperativity for cucurbit[8]uril (CB[8]) mediated ternary complexation is required for understanding and advance designs of such ternary self-assembled systems. A key issue is to dissect the contributions of the binding steps of the first and second guest molecules to the overall ternary complex formation energy. This is addressed by performing concentration-dependent titrations between CB[8] and guests by means of concentration-dependent calorimetric and 1H-NMR titrations. The sensitivity of the fitting of the cumulative heat of complexation of the calorimetric titrations is evaluated in terms of fitting error and enthalpy-entropy compensation and, together with the NMR spectroscopic analysis of the separate species, non-cooperative binding is conceived to be the most probable binding scenario...
February 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28194936/supramolecular-chemotherapy-cooperative-enhancement-of-antitumor-activity-by-combining-controlled-release-of-oxaliplatin-and-consuming-of-spermine-by-cucurbit-7-uril
#10
Yueyue Chen, Zehuan Huang, Hanyang Zhao, Jiangfei Xu, Zhiwei Sun, Xi Zhang
Supramolecular chemotherapy is aimed to employ supramolecular approach for regulating the cytotoxicity and improving the efficiency of antitumor drugs. In this paper, we demonstrated a new example of supramolecular chemotherapy by utilizing the clinical antitumor drug, oxaliplatin, which is the specific drug for colorectal cancer treatment. Cytotoxicity of oxaliplatin to the colorectal normal cell could be significantly decreased by host-guest complexation between oxaliplatin and cucurbit[7]uril (CB[7]). More importantly, oxaliplatin-CB[7] exhibited cooperatively enhanced antitumor activity than oxaliplatin itself...
February 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28193355/a-paper-based-nanomodified-electrochemical-biosensor-for-ethanol-detection-in-beers
#11
Stefano Cinti, Mattia Basso, Danila Moscone, Fabiana Arduini
Herein, we report the first example of a paper-based screen-printed biosensor for the detection of ethanol in beer samples. Common office paper was adopted to fabricate the analytical device. The properties of this paper-based screen-printed electrode (SPE) were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy, and they were compared with the well-established polyester-based SPEs as well. Paper demonstrated similar properties when compared with polyester, highlighting suitability towards its utilization in sensor development, with the advantages of low cost and simple disposal by incineration...
April 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28192651/current-rectification-through-vertical-heterojunctions-between-two-single-layer-dichalcogenides-wse2-mos2-pn-junctions
#12
Hrishikesh Bhunia, Abhijit Bera, Amlan J Pal
We form junctions between two single layers of p-type WSe2 and n-type MoS2 in both sequences. The WSe2|MoS2 and MoS2|WSe2 junctions of ultimate thickness limit exhibit current rectification when characterized vertically with a scanning tunneling microscope (STM) tip. The direction of rectification in the pn-junction is opposite to that of the np-junction confirming occurrence of the rectification to be due to the junctions themselves. From scanning tunneling spectroscopy (STS) and correspondingly the density of states (DOS), we locate the conduction and valence band-edges (CB and VB, respectively) of the materials inferring their single-layer and 2H phase configuration...
February 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28192351/application-of-coupled-zero-valent-iron-biochar-system-for-degradation-of-chlorobenzene-contaminated-groundwater
#13
Xu Zhang, Yanqing Wu
A novel iron-carbon micro-electrolysis system, bamboo-derived biochar coupled with zero-valent iron (ZVI), was investigated for chlorobenzene (CB)-contaminated groundwater removal. Influences of initial pH value, mass ratio of the ZVI/Biochar, initial CB concentration and ionic strength of the ZVI/Biochar micro-electrolysis were studied. The results indicated that the increase of initial pH led to the decrease of the CB removal efficiency. While the optimum mass ratio of ZVI to biochar was 2:1, the improved initial concentration and reaction time were 33...
February 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28191815/bladder-cancer-cell-growth-and-motility-implicate-cannabinoid-2-receptor-mediated-modifications-of-sphingolipids-metabolism
#14
Arianna Bettiga, Massimo Aureli, Giorgia Colciago, Valentina Murdica, Marco Moschini, Roberta Lucianò, Daniel Canals, Yusuf Hannun, Petter Hedlund, Giovanni Lavorgna, Renzo Colombo, Rosaria Bassi, Maura Samarani, Francesco Montorsi, Andrea Salonia, Fabio Benigni
The inhibitory effects demonstrated by activation of cannabinoid receptors (CB) on cancer proliferation and migration may also play critical roles in controlling bladder cancer (BC). CB expression on human normal and BC specimens was tested by immunohistochemistry. Human BC cells RT4 and RT112 were challenged with CB agonists and assessed for proliferation, apoptosis, and motility. Cellular sphingolipids (SL) constitution and metabolism were evaluated after metabolic labelling. CB1-2 were detected in BC specimens, but only CB2 was more expressed in the tumour...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28191761/angiogenic-potential-of-human-bone-marrow-derived-mesenchymal-stem-cells-in-chondrocyte-brick-enriched-constructs-promoted-stable-regeneration-of-craniofacial-cartilage
#15
Zhiye Li, Ruikai Ba, Zhifa Wang, Jianhua Wei, Yimin Zhao, Wei Wu
Craniofacial deformities caused by congenital defects or trauma remain challenges for clinicians, whereas current surgical interventions present limited therapeutic outcomes. Injection of bone marrow-derived mesenchymal stem cells (BMSCs) into the defect is highly desirable because such a procedure is microinvasive and grafts are more flexible to fill the lesions. However, preventing hypertrophic transition and morphological contraction remain significant challenges. We have developed an "all host derived" cell transplantation system composed of chondrocyte brick (CB)-enriched platelet-rich plasma (P) gel and BMSCs (B)...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191616/removal-of-pb-zn-and-cd-from-contaminated-soil-by-new-washing-agent-from-plant-material
#16
Yaru Cao, Shirong Zhang, Guiyin Wang, Qinling Huang, Ting Li, Xiaoxun Xu
Soil washing is an effective approach to remove soil heavy metals, and the washing agent is generally regarded as one of the primary factors in the process, but there is still a lack of efficient and eco-friendly agents for this technique. Here, we showed that four plant washing agents-from water extracts of Coriaria nepalensis (CN), Clematis brevicaudata (CB), Pistacia weinmannifolia (PW), and Ricinus communis (RC)-could be feasible agents for the removal of soil lead (Pb), zinc (Zn), and cadmium (Cd). The metal removal efficiencies of the agents increased with their concentrations from 20 to 80 g L(-1), decreased with the increasing solution pH, and presented different trends with the reaction time increasing...
February 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28187825/characterization-of-acetylcholinesterase-from-elm-left-beetle-xanthogaleruca-luteola-and-qsar-of-temephos-derivatives-against-its-activity
#17
Mahboobeh Sharifi, Mohammad Ghadamyari, Khodayar Gholivand, Ali Asghar Ebrahimi Valmoozi, Reza H Sajedi
Insect acetylcholinesterase (AChE) is the principal target for organophosphate (OP) and carbamate (CB) insecticides. In this research, an AChE from third instar larvae of elm left beetle, Xanthogaleruca luteola was purified by affinity chromatography. The enzyme was purified 75.29-fold with a total yield of 8.51%. As shown on denaturing SDS-PAGE, the molecular mass of purified AChE was 70kDa. The enzyme demonstrated maximum activity at pH7 and 35°C. Furthermore, a series of temephos (Tem) derivatives with the general structure of P(O)XP(O) (1-44) were prepared, synthesized and characterized by (31)P, (13)C, (1)H NMR and FT-IR spectral techniques...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28187462/recombinant-tat-bmi-1-fusion-protein-induces-ex-vivo-expansion-of-human-umbilical-cord-blood-derived-hematopoietic-stem-cells
#18
Bruna Codispoti, Nicola Rinaldo, Emanuela Chiarella, Michela Lupia, Cristina Barbara Spoleti, Maria Grazia Marafioti, Annamaria Aloisio, Stefania Scicchitano, Marco Giordano, Giovanna Nappo, Valeria Lucchino, Malcolm A S Moore, Pengbo Zhou, Maria Mesuraca, Heather Mandy Bond, Giovanni Morrone
Transplantation of hematopoietic stem cells (HSCs) is a well-established therapeutic approach for numerous disorders. HSCs are typically derived from bone marrow or peripheral blood after cytokine-induced mobilization. Umbilical cord blood (CB) represents an appealing alternative HSC source, but the small amounts of the individual CB units have limited its applications. The availability of strategies for safe ex vivo expansion of CB-derived HSCs (CB-HSCs) may allow to extend the use of these cells in adult patients and to avoid the risk of insufficient engraftment or delayed hematopoietic recovery...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28186862/comparison-of-conventional-syringe-canalbrush-endoactivator-photon-induced-photoacoustic-streaming-and-manual-instrumentation-in-removing-orange-brown-precipitate-an-in-vitro-study
#19
Mehmet Burak Guneser, Asiye Nur Dincer, Dilara Arslan
OBJECTIVE: The aim of this in vitro study was to compare the various techniques for removing precipitate formed after irrigation with sodium hypochlorite (NaOCl) and chlorhexidine (CHX). BACKGROUND DATA: It is still unclear whether different irrigant activation systems can effectively remove precipitate that is firmly attached to the root canal walls. METHODS: Eighty-two extracted single-rooted human teeth were instrumented by using the ProTaper rotary system...
February 10, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28182422/unraveling-the-structure-affinity-relationship-between-cucurbit-n-urils-n-7-8-and-cationic-diamondoids
#20
David Sigwalt, Marina Šekutor, Liping Cao, Peter Y Zavalij, Jiří Hostaš, Haresh Ajani, Pavel Hobza, Kata Mlinarić-Majerski, Robert Glaser, Lyle Isaacs
We report measurement of the binding constants (Ka) for cucurbit[n]uril (n = 7, 8) toward four series of guests based on 2,6-disubstituted adamantanes, 4,9-disubstituted diamantanes, 1,6-disubstituted diamantanes, and 1-substituted adamantane ammonium ions by direct and competitive 1H NMR spectroscopy. Compared to CB[7]•Diam(NMe3)2, the adamantane diammonium ion complexes (e.g. CB[7]•2,6-Ad(NH3)2 and CB[7]•2,6-Ad(NMe3)2 are less effective at realizing the potential 1000-fold enhancement in affinity due to ion dipole interactions at the second ureidyl C=O portal...
February 9, 2017: Journal of the American Chemical Society
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