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nogo b

Olli Rämö, Darshan Kumar, Erika Gucciardo, Merja Joensuu, Maiju Saarekas, Helena Vihinen, Ilya Belevich, Olli-Pekka Smolander, Kui Qian, Petri Auvinen, Eija Jokitalo
Reticulons (RTNs) are a large family of membrane associated proteins with various functions. NOGO-A/RTN4A has a well-known function in limiting neurite outgrowth and restricting the plasticity of the mammalian central nervous system. On the other hand, Reticulon 4 proteins were shown to be involved in forming and maintaining endoplasmic reticulum (ER) tubules. Using comparative transcriptome analysis and qPCR, we show here that NOGO-B/RTN4B and NOGO-A/RTN4A are simultaneously expressed in cultured epithelial, fibroblast and neuronal cells...
October 27, 2016: Scientific Reports
Jagna Sobierajewicz, Anna Przekoracka-Krawczyk, Wojciech Jaśkowski, Willem B Verwey, Rob van der Lubbe
Motor imagery has been argued to affect the acquisition of motor skills. The present study examined the specificity of motor imagery on the learning of a fine hand motor skill by employing a modified discrete sequence production task: the Go/NoGo DSP task. After an informative cue, a response sequence had either to be executed, imagined, or withheld. To establish learning effects, the experiment was divided into a practice phase and a test phase. In the latter phase, we compared mean response times and accuracy during the execution of unfamiliar sequences, familiar imagined sequences, and familiar executed sequences...
October 6, 2016: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
Iris Völker, Iris Schwarze, Tina E Brezina, Saskia Köstlinger, Marion Hewicker-Trautwein
An 8-month-old, male African hedgehog clinically displayed a wobbly walk, anuria, inappetence and apathy, whereupon the suspected diagnosis wobbly hedgehog syndrome was made. After exacerbation, the hedgehog was euthanized. Histologically, a tumour mainly consisting of medium-sized, oval tumour cells and a smaller number of spindeloid cells was found in the cerebrum. The tumour contained neuropil islets and extracellular myxoid material. Immunohistochemically, the tumour cells expressed oligodendroglial (neurite outgrowth inhibitor, Nogo-A; oligodendrocyte transcription factor, Olig-2) and neuronal (neuron-specific enolase, NSE; microtubule-associated protein-2a, MAP-2a; synaptophysin) cell markers...
October 12, 2016: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
Linda Sasset, Yi Zhang, Teresa M Dunn, Annarita Di Lorenzo
Sphingolipids (SL) are both fundamental structural components of the eukaryotic membranes and signaling molecules that regulate a variety of biological functions. The highly-bioactive lipids, ceramide and sphingosine-1-phosphate, have emerged as important regulators of cardiovascular function in health and disease. In this review we discuss recent insights into the role of SLs, particularly ceramide and sphingosine-1-phosphate, in the pathophysiology of the cardiovascular system. We also highlight advances into the molecular mechanisms regulating serine palmitoyltransferase, the first and rate-limiting enzyme of de novo SL biosynthesis, with an emphasis on the recently discovered inhibitors of serine palmitoyltransferase, ORMDL and NOGO-B proteins...
November 2016: Trends in Endocrinology and Metabolism: TEM
Johanna Piispala, Mika Kallio, Risto Bloigu, Eira Jansson-Verkasalo
PURPOSE OF THE STUDY: The main aim of the study was to investigate the attentional and inhibitory abilities and their underlying processes of children who stutter by using behavioural measurement and event-related potentials (ERP) in a visual Go/Nogo paradigm. METHODS: Participants were 11 children who stutter (CWS; mean age 8.1, age range 6.3-9.5 years) and 19 typically developed children (TDC; mean age 8.1, age range 5.8-9.6 years). They performed a visual Go/Nogo task with simultaneous EEG recording to obtain ERP responses...
June 2016: Journal of Fluency Disorders
Yu-Chao Zuo, Nan-Xiang Xiong, Jian-Ying Shen, Hua Yu, Yi-Zhi Huang, Hong-Yang Zhao
The ability of neurons in the adult mammalian central nervous system (CNS) to regenerate after injury is limited by inhibitors in CNS myelin. Nogo-66 is the most important myelin inhibitor but the mechanisms of Nogo-66 inhibition of neurite outgrowth remain poorly understood. Particularly, the relationship between Nogo-66 and microtubule-affinity regulating kinase 2 (MARK2) has not been examined. This study investigated the role of MARK2 in Nogo-66 inhibition and the function of MARK2 in neurite elongation in neurons in vitro...
July 28, 2016: Neurochemical Research
Aaron S Heller, Alexandra O Cohen, Michael F W Dreyfuss, B J Casey
The capacity to suppress inappropriate thoughts, emotions and actions in favor of appropriate ones shows marked changes throughout childhood and adolescence. Most research has focused on pre-frontal circuit development to explain these changes. Yet, subcortical circuitry involving the amygdala and ventral striatum (VS) has been shown to modulate cue-triggered motivated behaviors in rodents. The nature of the interaction between these two subcortical regions in humans is less well understood, especially during development when there appears to be heightened sensitivity to emotional cues...
December 2016: Social Cognitive and Affective Neuroscience
Jacek Calik, Bartosz Pula, Aleksandra Piotrowska, Andrzej Wojnar, Wojciech Witkiewicz, Jedrzej Grzegrzolka, Marzena Podhorska-Okolow, Piotr Dziegiel
BACKGROUND: Neurite outgrowth inhibitor type B (NOGO-B) and its receptor (NGBR) were shown to regulate various crucial cellular processes and may be therefore potential factors influencing carcinogenesis. MATERIALS AND METHODS: Expression of NOGO-A, NOGO-A/B and NGBR was studied in benign melanocytic lesions and primary tumors and metastases of malignant melanoma (MM). RESULTS: Cytoplasmic expression of the studied antigens was detected in melanocytes and MM cells...
July 2016: Anticancer Research
Guy A Higgins, Leo B Silenieks, Everett B Altherr, Cam MacMillan, Paul J Fletcher, Wayne E Pratt
RATIONALE: The 5-HT2C receptor agonist lorcaserin (Belviq®) has been approved by the FDA for the treatment of obesity. Impulsivity is a contributory feature of some eating disorders. OBJECTIVE: Experiments investigated the effect of lorcaserin and the highly selective 5-HT2C agonist CP-809101 on measures of impulsivity and on reinstatement of food-seeking behaviour, a model of dietary relapse. The effect of both drugs on 22-h deprivation-induced feeding was also examined, as was the effect of prefeeding in each impulsivity test...
July 2016: Psychopharmacology
A W S Metcalfe, B J MacIntosh, A Scavone, X Ou, D Korczak, B I Goldstein
Executive dysfunction is common during and between mood episodes in bipolar disorder (BD), causing social and functional impairment. This study investigated the effect of acute exercise on adolescents with BD and healthy control subjects (HC) to test for positive or negative consequences on neural response during an executive task. Fifty adolescents (mean age 16.54±1.47 years, 56% female, 30 with BD) completed an attention and response inhibition task before and after 20 min of recumbent cycling at ~70% of age-predicted maximum heart rate...
2016: Translational Psychiatry
Guy A Higgins, Leo B Silenieks, Cam MacMillan, Julia Sevo, Fiona D Zeeb, Sandy Thevarkunnel
NMDA GluN2B (NR2B) subtype selective antagonists are currently in clinical development for a variety of indications, including major depression. We previously reported the selective NMDA GluN2B antagonists Ro 63-1908 and traxoprodil, increase premature responding in a 5-choice serial reaction time task (5-CSRTT) suggesting an effect on impulsive action. The present studies extend these investigations to a Go-NoGo and delay discounting task, and the 5-CSRTT under test conditions of both regular (5s) and short (2-5s) multiple ITI (Intertrial interval)...
September 15, 2016: Behavioural Brain Research
Nianping Feng, Guang Hao, Fenggang Yang, Fujun Qu, Haihong Zheng, Songlan Liang, Yonghua Jin
Cerebral ischemia, which may lead to cerebral hypoxia and damage of the brain tissue, is a leading cause of human mortality and adult disability. Mesenchymal stem cells (MSCs) are a class of adult progenitor cells with the ability to differentiate into multiple cell types. The transplantation of bone marrow-derived MSCs is a potential therapeutic strategy for cerebral ischemia. However, the underlying mechanism has yet to be elucidated. In the present study, primary MSCs were isolated from healthy rats, labeled and transplanted into the brains of middle cerebral artery occlusion rat models...
May 2016: Experimental and Therapeutic Medicine
Yi Zhang, Yan Huang, Anna Cantalupo, Paula S Azevedo, Mauro Siragusa, Jacek Bielawski, Frank J Giordano, Annarita Di Lorenzo
We recently discovered that endothelial Nogo-B, a membrane protein of the ER, regulates vascular function by inhibiting the rate-limiting enzyme, serine palmitoyltransferase (SPT), in de novo sphingolipid biosynthesis. Here, we show that endothelium-derived sphingolipids, particularly sphingosine-1-phosphate (S1P), protect the heart from inflammation, fibrosis, and dysfunction following pressure overload and that Nogo-B regulates this paracrine process. SPT activity is upregulated in banded hearts in vivo as well as in TNF-α-activated endothelium in vitro, and loss of Nogo removes the brake on SPT, increasing local S1P production...
April 21, 2016: JCI Insight
Juan A Rodríguez-Feo, Julio Gallego-Delgado, Marta Puerto, Francisco Wandosell, Julio Osende
No abstract text is available yet for this article.
August 2016: Biochimica et Biophysica Acta
Iman K Yazdi, Nima Taghipour, Sarah Hmaidan, Roberto Palomba, Shilpa Scaria, Alvaro Munoz, Timothy B Boone, Ennio Tasciotti
The use of a monoclonal antibody to block the neurite outgrowth inhibitor Nogo-A has been of great interest for promoting axonal recovery as a treatment for spinal cord injury. While several cellular and non-cellular assays have been developed to quantify the bioactive effects of Nogo-A signaling, demand still exists for the development of a reliable approach to characterize the effectiveness of the anti-Nogo-A antibody. In this study, we developed and validated a novel cell-based approach to facilitate the biological quantification of a Nogo-A antibody using PC-12 cells as an in vitro neuronal cell model...
January 2016: Journal of Tissue Engineering
Chengyong Dong, Baofeng Zhao, Fei Long, Ying Liu, Zhenzhen Liu, Song Li, Xuejun Yang, Deguang Sun, Haibo Wang, Qinlong Liu, Rui Liang, Yan Li, Zhenming Gao, Shujuan Shao, Qing Robert Miao, Liming Wang
Nogo-B receptor (NgBR), a type I single transmembrane domain receptor is the specific receptor for Nogo-B. Our previous work demonstrated that NgBR is highly expressed in breast cancer cells, where it promotes epithelial mesenchymal transition (EMT), an important step in metastasis. Here, we show that both in vitro and in vivo increased expression of NgBR contributes to the increased chemoresistance of Bel7402/5FU cells, a stable 5-FU (5-Fluorouracil) resistant cell line related Bel7402 cells. NgBR knockdown abrogates S-phase arrest in Bel7402/5FU cells, which correlates with a reduction in G1/S phase checkpoint proteins p53 and p21...
February 23, 2016: Oncotarget
Ujala Rana, Zhong Liu, Suresh N Kumar, Baofeng Zhao, Wenquan Hu, Michelle Bordas, Stephanie Cossette, Sara Szabo, Jamie Foeckler, Hartmut Weiler, Magdalena Chrzanowska-Wodnicka, Mary L Holtz, Ravindra P Misra, Valerie Salato, Paula E North, Ramani Ramchandran, Qing Robert Miao
Nogo-B receptor (NgBR) was identified as a receptor specific for Nogo-B. Our previous work has shown that Nogo-B and its receptor (NgBR) are essential for chemotaxis and morphogenesis of endothelial cells in vitro and intersomitic vessel formation via Akt pathway in zebrafish. Here, we further demonstrated the roles of NgBR in regulating vasculature development in mouse embryo and primitive blood vessel formation in embryoid body culture systems, respectively. Our results showed that NgBR homozygous knockout mice are embryonically lethal at E7...
February 15, 2016: Developmental Biology
Atsushi Nanashima, Go Hatachi, Tetsurou Tominaga, Goushi Murakami, Katsunori Takagi, Junichi Arai, Hideo Wada, Takeshi Nagayasu, Yorihisa Sumida
Nogo-B, located in the endoplasmic reticulum, is an isoform belonging to the reticulon protein family, which is expressed specifically in cholangiocytes and non-parenchymal cells in the liver. Nogo-B expression is down-regulated with the progression of liver fibrosis, but its distinct function in liver malignancies has not been fully clarified. We have hypothesized that Nogo-B expression may be altered in intrahepatic cholangiocarcinoma (ICC), a relatively rare type of primary liver cancer with highly malignant behavior...
2016: Tohoku Journal of Experimental Medicine
Kent S Tadokoro, Ujala Rana, Xigang Jing, G Ganesh Konduri, Qing R Miao, Ru-Jeng Teng
Nogo-B and its receptor (NgBR) are involved in blood vessel growth in developing lungs, but their role in pulmonary artery smooth muscle cell (PASMC) growth is unknown. We hypothesized that NgBR regulates growth of PASMCs by modulating the function of endoplasmic reticulum (ER) and formation of reactive oxygen species (ROS). In utero constriction of the ductus arteriosus created pulmonary hypertension in fetal lambs (hypertensive fetal lamb [HTFL]). PASMCs isolated 8 days after surgery were assessed for the alteration of protein levels by immunoblots and ROS formation by dihydroethidium and Cell ROX deep red fluorescence...
June 2016: American Journal of Respiratory Cell and Molecular Biology
Christoph Eckharter, Nina Junker, Lilli Winter, Irmgard Fischer, Barbara Fogli, Steffen Kistner, Kristian Pfaller, Binhai Zheng, Gerhard Wiche, Lars Klimaschewski, Rüdiger Schweigreiter
UNLABELLED: In contrast to the central nervous system (CNS) nerve fibers do regenerate in the peripheral nervous system (PNS) although in a clinically unsatisfying manner. A major problem is excessive sprouting of regenerating axons which results in aberrant reinnervation of target tissue and impaired functional recovery. In the CNS, the reticulon protein Nogo-A has been identified as a prominent oligodendrocyte expressed inhibitor of long-distance growth of regenerating axons. We show here that the related isoform Nogo-B is abundantly expressed in Schwann cells in the PNS...
2015: Frontiers in Cellular Neuroscience
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