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food antibiotic residues

Thomas Bessaire, Claudia Mujahid, Andrea Beck, Adrienne Tarres, Marie-Claude Savoy, Pei-Mun Woo, Pascal Mottier, Aurélien Desmarchelier
A fast and robust high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) screening method was developed for the analysis of β-lactam antibiotics in foods of animal origin: eggs, raw milk, processed dairy ingredients, infant formula, and meat- and fish-based products including baby-foods. QuEChERS extraction with some adaptations enabled twenty-three drugs to be simultaneously monitored. Screening Target Concentrations were set at levels adequate to ensure compliance with current European, Chinese, US and Canadian regulations...
January 11, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Thierry Delatour, Lucie Racault, Thomas Bessaire, Aurélien Desmarchelier
Regulatory agencies and government authorities have established maximum residue limits (MRL) in various food matrices of animal origin for supporting governments and food operators in the monitoring of veterinary drug residues in the food chain, and ultimately in the consumer's plate. Today, about 200 veterinary drug residues from several families, mainly with antibiotic, antiparasitic or anti-inflammatory activities, are regulated in a variety of food matrices such as milk, meat or egg. The current article provides a review of the regulatory framework in milk and muscle including data from Codex Alimentarius, Europe, USA, Canada and China for about 220 veterinary drugs...
January 11, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Shengfeng Huang, Ning Gan, Tianhua Li, You Zhou, Yuting Cao, Youren Dong
An ultrasensitive electrochemical aptasensor for multiplex antibiotics detection based on endonuclease and exonuclease assisted dual recycling amplification strategy was proposed. Kanamycin and chloramphenicol were selected as candidates. Firstly, aptamers of the antibiotics were immobilized on bar A and then binding with their endonuclease labeled complementary DNA strands to construct enzyme-cleavage probes. Secondly, The nano zirconium-metal organic framework (NMOF) particles with 1,4-benzene-dicarboxylate (BDC) as linker was defined as UiO-66...
March 1, 2018: Talanta
Xiaolu Yu, Hang Liu, Chengjun Pu, Junhao Chen, Ying Sun, Lin Hu
A modified quick, easy, cheap, effective, rugged and safe method was established for simultaneous extraction and cleanup of multiple antibiotics in leafy vegetables, and ultra high performance liquid chromatography with tandem mass spectrometry was used for analysis. Antibiotics in leafy vegetables were extracted with citric acid/sodium citrate in mixed solvents consisting of acetonitrile/methanol (85:15 v/v) from 10 g of vegetables. Octadecylsilyl and graphitized carbon black were used as dispersant adsorbents...
November 14, 2017: Journal of Separation Science
Fausto Scoppetta, Marco Sensi, Maria Pia Franciosini, Marinella Capuccella
Antibiotic use in food-producing animals has considerable impact on public health, especially with respect to the development and spread of antibiotic resistance. Pigs represent one of the main species in which antibiotics are frequently used for different purposes. Surveillance of antibiotic consumption and dose appropriateness, through novel approaches based on defined daily doses, is strongly needed to assess farms' antibiotic risk, in terms of spread of antibiotic resistance and possibile presence of residues in meat...
August 16, 2017: Italian Journal of Food Safety
A H C Van Bruggen, M M He, K Shin, V Mai, K C Jeong, M R Finckh, J G Morris
The herbicide glyphosate, N-(phosphonomethyl) glycine, has been used extensively in the past 40years, under the assumption that side effects were minimal. However, in recent years, concerns have increased worldwide about the potential wide ranging direct and indirect health effects of the large scale use of glyphosate. In 2015, the World Health Organization reclassified glyphosate as probably carcinogenic to humans. A detailed overview is given of the scientific literature on the movement and residues of glyphosate and its breakdown product aminomethyl phosphonic acid (AMPA) in soil and water, their toxicity to macro- and microorganisms, their effects on microbial compositions and potential indirect effects on plant, animal and human health...
November 5, 2017: Science of the Total Environment
Axel Mie, Helle Raun Andersen, Stefan Gunnarsson, Johannes Kahl, Emmanuelle Kesse-Guyot, Ewa Rembiałkowska, Gianluca Quaglio, Philippe Grandjean
This review summarises existing evidence on the impact of organic food on human health. It compares organic vs. conventional food production with respect to parameters important to human health and discusses the potential impact of organic management practices with an emphasis on EU conditions. Organic food consumption may reduce the risk of allergic disease and of overweight and obesity, but the evidence is not conclusive due to likely residual confounding, as consumers of organic food tend to have healthier lifestyles overall...
October 27, 2017: Environmental Health: a Global Access Science Source
Steven J Lehotay, Alan R Lightfield
The way to maximize scope of analysis, sample throughput, and laboratory efficiency in the monitoring of veterinary drug residues in food animals is to determine as many analytes as possible as fast as possible in as few methods as possible. Capital and overhead expenses are also reduced by using fewer instruments in the overall monitoring scheme. Traditionally, the highly polar aminoglycoside antibiotics require different chromatographic conditions from other classes of drugs, but in this work, we demonstrate that an ion-pairing reagent (sodium 1-heptanesulfonate) added to the combined final extracts from two sample preparation methods attains good separation of 174 targeted drugs, including 9 aminoglycosides, in the same 10...
October 23, 2017: Analytical and Bioanalytical Chemistry
Hedieh Attai, Jeanette Rimbey, George P Smith, Pamela J B Brown
In order to provide food security, innovative approaches to preventing plant disease are currently being explored. Here, we demonstrate that lytic bacteriophages and phage lysis proteins are effective at triggering lysis of the phytopathogen Agrobacterium tumefaciens Phages Atu_ph02 and Atu_ph03 were isolated from wastewater and induce lysis of C58-derived strains of A. tumefaciens Co-inoculation of A. tumefaciens with phage onto potato discs limits tumor formation. The genomes of Atu_ph02 and Atu_ph03 are nearly identical and ∼42% identical to T7 supercluster phages...
September 29, 2017: Applied and Environmental Microbiology
Bixia Lin, Ying Yu, Yujuan Cao, Manli Guo, Debin Zhu, Jiaxing Dai, Minshi Zheng
The rapid detection of antibiotic residual in everyday life is very important for food safety. In order to realize the on-site and visual detection of antibiotic, a POCT method was established by using digital image colorimetry based on smartphone. Streptomycin was taken as the analyte model of antibiotics, streptomycin aptamer preferentially recognized analyte, and the excess aptamer hybridized with the complementary DNA to form the dsDNA. SYBR Green I combined with the dsDNA and then emitted obvious green fluorescence, thus the fluorescence intensity decreased with the increasing of streptomycin concentration...
September 20, 2017: Biosensors & Bioelectronics
Jon Ashley, Kaiyu Wu, Mikkel Fougt Hansen, Michael Stenbæk Schmidt, Anja Boisen, Yi Sun
There is an increasing demand for rapid, sensitive, and low cost analytical methods to routinely screen antibiotic residues in food products. Conventional detection of antibiotics involves sample preparation by liquid-liquid or solid-phase extraction, followed by analysis using liquid chromatography-mass spectrometry (LC-MS), capillary electrophoresis (CE), or gas chromatography (GC). The process is labor-intensive, time-consuming, and expensive. In this study, we developed a new analytical method that combines magnetic molecularly imprinted polymer (MMIP)-based sample preparation with surface-enhanced Raman spectroscopy (SERS)-based detection for quantitative analysis of cloxacillin in pig serum...
November 7, 2017: Analytical Chemistry
Luca Maria Chiesa, Maria Nobile, Renato Malandra, Sara Panseri, Francesco Arioli
Filter feeders, like mussels and clams, are suitable bioindicators of environmental pollution. These shellfish, when destined for human consumption, undergo a depuration step that aims to nullify their pathogenic microorganism load and decrease chemical contamination. Nevertheless, the lack of contamination by drugs may not be guaranteed. Antimicrobials are a class of drugs of particular concern due to the increasing phenomenon of antibiotic resistance. Their use in breeding and aquaculture is a major cause of this...
February 1, 2018: Food Chemistry
Maider Greño, María Castro-Puyana, María Ángeles García, María Luisa Marina
Natural, synthetic or semisynthetic antibiotics are highly used to prevent or treat diseases in humans and animals, and to promote animal growth. This fact makes that antibiotics residues or their transformation products may be present in food or in the environment after human or animal excretion. For this reason, it is imperative to develop reliable and sensitive analytical methodologies for their analysis. The main aim of this work is to present and discuss the most recent applications of capillary electromigration methods for the analysis of antibiotics, including the developments and applications of their use as chiral selectors in CE...
September 23, 2017: Electrophoresis
Keena Ae Mullen, Erin Beasley, Julio Q Rizzo, Steven P Washburn, Ronald E Baynes, Sharon E Mason, Kevin L Anderson
Mastitis is a costly disease for dairy farmers. Some dairy farmers use herbal products, or phytoceuticals, to treat mastitis. Phytoceuticals have not been approved for this use by the United States Food and Drug Administration, and have not been tested to determine how they impact antibiotic residue detection testing. The current study tested the potential for phytoceuticals to cause positive results on two milk antibiotic residue screening tests, the Delvotest P and Charm SL Beta-lactam test, or to interfere with the detection of antibiotics by these tests...
2017: Veterinary Record Open
Na Li, Keith W K Ho, Guang-Guo Ying, Wen-Jing Deng
Due to the harmful effects of veterinary antibiotics (VAs) residues in food on children's health, urine samples from 31 preschool and primary school children were analyzed for 13 common VAs. Samples of raw and cooked pork, chicken, fish, milk and drinking water from the children's living areas were also analyzed for residual VAs. Urinalysis revealed one to four target antibiotics in 77.4% of the sample group, with concentrations as high as 0.36ng/mL. Norfloxacin and penicillin had the highest detection rates (48...
November 2017: Environment International
Kuppan Gokulan, Carl E Cerniglia, Charles Thomas, Silvia A Pineiro, Sangeeta Khare
Tetracyclines are frequently used in food-producing animals to treat, control, and prevent microbial diseases. Concerns are raised regarding the effects of residual levels of tetracycline, which may be present in the food supply, for emergence of drug-resistance and transfer of antibiotic-resistance gene in intestinal microbiota. In contrast, no information is available regarding the possible effects of residual tetracycline on the gastrointestinal epithelial layer barrier-disruption. This study investigates the outcome of tetracycline treatment on intestinal epithelial cells integrity...
September 4, 2017: Food and Chemical Toxicology
Andrea Osimani, Lucia Aquilanti, Marina Pasquini, Francesca Clementi
The thermotolerant species Campylobacter jejuni, Campylobacter coli, Campylobacter lari and Campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. This infection represents a threat for the health of consumers in Europe. It is well known that poultry meat is an important food vehicle of Campylobacter infection. As emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in Europe represents an important source of the pathogen...
September 1, 2017: Poultry Science
Ting Chen, Guyue Cheng, Saeed Ahmed, Yulian Wang, Xu Wang, Haihong Hao, Zonghui Yuan
Antibiotics are leading medicine asset for fighting against microbial infection, but also one of the important causes of death worldwide. Many antibiotics used as therapeutics and growth promotion agents in animals can lead to antibiotic residues in animal-derived food which harm the health of people. Hence, it is vital to screen antibiotic residues in animal derived foods. Typical methods for screening antibiotic residues are based on microbiological growth inhibition and immunological analyses. However these two methods have some disadvantages, such as poor sensitive, lack of specificity and etc...
December 1, 2017: Talanta
Kohei Kitazaki, Shoko Koga, Kohei Nagatoshi, Koichi Kuwano, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto, Hitoshi Ano, Hiromu Katamoto
First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. Therefore, a combination of CEZ and nisin A might provide an extended activity spectrum against mastitis pathogens and reduce the antibiotic dose for mastitis treatment...
September 12, 2017: Journal of Veterinary Medical Science
Anastasis Christou, Ana Agüera, Josep Maria Bayona, Eddie Cytryn, Vasileios Fotopoulos, Dimitra Lambropoulou, Célia M Manaia, Costas Michael, Mike Revitt, Peter Schröder, Despo Fatta-Kassinos
The use of reclaimed wastewater (RWW) for the irrigation of crops may result in the continuous exposure of the agricultural environment to antibiotics, antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In recent years, certain evidence indicate that antibiotics and resistance genes may become disseminated in agricultural soils as a result of the amendment with manure and biosolids and irrigation with RWW. Antibiotic residues and other contaminants may undergo sorption/desorption and transformation processes (both biotic and abiotic), and have the potential to affect the soil microbiota...
October 15, 2017: Water Research
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