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https://www.readbyqxmd.com/read/28291385/determination-of-oxytetracycline-and-4-epi-oxytetracycline-residues-in-feathers-and-edible-tissues-of-broiler-chickens-using-liquid-chromatography-coupled-with-tandem-mass-spectrometry
#1
Javiera Cornejo, Ekaterina Pokrant, Magdalena Krogh, Cristóbal Briceño, Héctor Hidalgo, Aldo Maddaleno, Carolina Araya-Jordán, Betty San Martín
Antibiotics have been widely used in poultry production for the treatment of bacterial diseases. However, drug residues can remain in products derived from animals after the cessation of the drug therapies. Feathers, in particular, have shown an affinity for antibiotics such as tetracycline, suggesting the persistence of these drugs in nonedible tissue. After the birds are slaughtered, feathers are ground into feather meals, which are used as organic fertilizer or an ingredient in animal diets, thereby entering into the food chain and becoming a potential risk for public health...
March 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28274457/detection-of-kanamycin-and-gentamicin-residues-in-animal-derived-food-using-igy-antibody-based-ic-elisa-and-fpia
#2
Cui Li, Yaoyao Zhang, Sergei A Eremin, Omar Yakup, Gang Yao, Xiaoying Zhang
Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50 of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC50 of FPIA and ic-ELISA based anti-Kana IgY were 7...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28224246/comparison-of-veterinary-drug-residue-results-in-animal-tissues-by-ultrahigh-performance-liquid-chromatography-coupled-to-triple-quadrupole-or-quadrupole-time-of-flight-tandem-mass-spectrometry-after-different-sample-preparation-methods-including-use-of-a-commercial
#3
Tarun Anumol, Steven J Lehotay, Joan Stevens, Jerry Zweigenbaum
Veterinary drug residues in animal-derived foods must be monitored to ensure food safety, verify proper veterinary practices, enforce legal limits in domestic and imported foods, and for other purposes. A common goal in drug residue analysis in foods is to achieve acceptable monitoring results for as many analytes as possible, with higher priority given to the drugs of most concern, in an efficient and robust manner. The U.S. Department of Agriculture has implemented a multiclass, multi-residue method based on sample preparation using dispersive solid phase extraction (d-SPE) for cleanup and ultrahigh-performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-QQQ) for analysis of >120 drugs at regulatory levels of concern in animal tissues...
February 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28218830/rapidfire-mass-spectrometry-with-enhanced-throughput-as-an-alternative-to-liquid-liquid-salt-assisted-extraction-and-lc-ms-analysis-for-sulfonamides-in-honey
#4
Brian T Veach, Thilak K Mudalige, Peter Rye
The use of veterinary drugs in honey bees for the prevention of infectious disease is ever increasing due to the spread of colony collapse disorder around the world. The United States Food and Drug Administration is concerned about the presence of these drugs residues in honey as they often lead to health concerns or potential antibiotic resistance. Currently there is a need for a rapid screening method for the detection of veterinary drugs in honey. Herein is a method that utilizes automated solid-phase extraction that is directly coupled to a mass spectrometer for the quantitative screening of 12 different regulated sulfonamides in honey...
March 2, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28213220/receptor-based-screening-assays-for-the-detection-of-antibiotics-residues-a-review
#5
REVIEW
Saeed Ahmed, Jianan Ning, Guyue Cheng, Ijaz Ahmad, Jun Li, Liu Mingyue, Wei Qu, Mujahid Iqbal, M A B Shabbir, Zonghui Yuan
Consumer and regulatory agencies have a high concern to antibiotic residues in food producing animals, so appropriate screening assays of fast, sensitive, low cost, and easy sample preparation for the identification of these residues are essential for the food-safety insurance. Great efforts in the development of a high-throughput antibiotic screening assay have been made in recent years. Concerning the screening of antibiotic residue, this review elaborate an overview on the availability, advancement and applicability of antibiotic receptor based screening assays for the safety assessment of antibiotics usage (i...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28208296/ultrasensitive-self-powered-aptasensor-based-on-enzyme-biofuel-cell-and-dna-bioconjugate-a-facile-and-powerful-tool-for-antibiotic-residue-detection
#6
Panpan Gai, Chengcheng Gu, Ting Hou, Feng Li
Herein, we reported a novel ultrasensitive one-compartment enzyme biofuel cells (EBFCs)-based self-powered aptasensing platform for antibiotic residue detection. By taking full advantage of the unique features of both EBFCs-based self-powered sensors and aptamers, the as-proposed aptasensing platform has the merits of simple instrumentation, anti-interference ability, high selectivity, and low cost. In this study, DNA bioconjugate, i.e., SiO2@gold nanoparticles-complementary strand of aptamer (SiO2@AuNPs-csDNA), was elaborately designed and played a key role in blocking the mass transport of glucose to the bioanode...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28187378/development-of-an-analytical-method-for-detecting-nitrofurans-in-bee-pollen-by-liquid-chromatography-electrospray-ionization-tandem-mass-spectrometry
#7
Mi Sun Park, Kyung Tae Kim, Jong Seong Kang
Bee pollen collected by honeybees, which is in powdered form, is a good nutritional supplement. Nitrofuran antibiotics are assumed not to be present in bee pollen, which is important as the level of antibiotics in bee pollen is strongly regulated in many countries. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect nitrofurans in honey has been developed, but this method is not suitable for bee pollen because of it being in powdered form. During preparation of bee pollen samples, the dispersal of powder particles in an aqueous solution often makes them susceptible to forming an emulsion with solvent components such as hexane and ethyl acetate...
January 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28177414/fate-and-effect-of-antibiotics-in-beef-and-dairy-manure-during-static-and-turned-composting
#8
Partha Ray, Chaoqi Chen, Katharine F Knowlton, Amy Pruden, Kang Xia
Manure composting has general benefits for production of soil amendment, but the effects of composting on antibiotic persistence and effects of antibiotics on the composting process are not well-characterized, especially for antibiotics commonly used in dairy cattle. This study provides a comprehensive, head-to-head, replicated comparison of the effect of static and turned composting on typical antibiotics used in beef and dairy cattle in their actual excreted form and corresponding influence on composting efficacy...
January 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28168081/tetracyclines-in-food-and-feedingstuffs-from-regulation-to-analytical-methods-bacterial-resistance-and-environmental-and-health-implications
#9
REVIEW
Fabio Granados-Chinchilla, César Rodríguez
Antibiotics are widely used as growth promoters in animal husbandry; among them, the tetracyclines are a chemical group of relevance, due to their wide use in agriculture, surpassing in quantities applied almost every other antibiotic family. Seeing the considerable amounts of tetracyclines used worldwide, monitoring of these antibiotics is paramount. Advances must be made in the analysis of antibiotics to assess correct usage and dosage of tetracyclines in food and feedstuffs and possible residues in pertinent environmental samples...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28120013/secretory-expression-and-surface-display-of-a-new-and-biologically-active-single-chain-insulin-sci-59-analog-by-lactic-acid-bacteria
#10
Ruifeng Mao, Dongli Wu, Shimeng Hu, Kangping Zhou, Man Wang, Yefu Wang
Insulin plays an important role in drug therapies for diabetes mellitus and as the main route of insulin delivery, subcutaneous injection may cause local discomfort, hypoglycemia, hyperinsulinemia, and patient non-compliance. Therefore, oral delivery of insulin is more preferred. However, there is a low bioavailability due to insulin degradation by proteolytic enzymes and severe pH conditions along the gastrointestinal tract. In order to use the food-grade bacteria lactic acid bacteria (LAB) as oral delivery vehicles, a new and bioactive single-chain insulin (SCI-59) analog, containing the insulin B- and A-chains connected by an eight-residue linker (RSRGLPFR), was secretory expressed in Lactococcus lactis NZ3900 without using an antibiotic resistance gene and displayed onto the surface of various non-viable bacteria (NVBs) without genetic modification...
January 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28054670/ultra-sensitive-detection-of-kanamycin-for-food-safety-using-a-reduced-graphene-oxide-based-fluorescent-aptasensor
#11
Na-Reum Ha, In-Pil Jung, Im-Joung La, Ho-Sup Jung, Moon-Young Yoon
Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28050849/glycin-rich-antimicrobial-peptide-yd1-from-b-amyloliquefaciens-induced-morphological-alteration-in-and-showed-affinity-for-plasmid-dna-of-e-coli
#12
Md Saifur Rahman, Yun Hee Choi, Yoon Seok Choi, Jin Cheol Yoo
Antimicrobial peptides (AMPs), low-molecular-weight proteins with broad-spectrum antimicrobial activity, are the most promising candidates for the development of novel antimicrobials. A powerful cationic glycine-rich AMP YD1 (MW ~ 1.0 kDa) was purified from Bacillus amyloliquefaciens CBSYD1 isolated from traditional Korean fermented food kimchi, for the treatment of multidrug-resistant (MDR) bacteria. Strain CBSYD1 was identified 99.79% similar to Bacillus amyloliquefaciens subsp. plantarum FZB42(T) by 16S rRNA sequence analysis...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28035803/molecularly-imprinted-membrane-electrospray-ionization-for-direct-sample-analyses
#13
Tianyi Li, Liusheng Fan, Yingfeng Wang, Xuebin Huang, Jianguo Xu, Jinxing Lu, Mei Zhang, Wei Xu
Typically dealing with practical samples with very complex matrices, ambient ionization mass spectrometry suffers from low detection sensitivity. In this study, molecular imprinting technology was explored and integrated with the membrane electrospray ionization (MESI) method for direct sample analyses. By enriching targeted analytes on molecularly imprinted membranes (MIMs), improvement (by 10- to 50-fold) in the limit of quantitation could be achieved, compared to conventional nanoelectrospray ionization methods or other ambient ionization methods...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/27979167/simultaneous-detection-of-four-nitrofuran-metabolites-in-honey-by-using-a-visualized-microarray-screen-assay
#14
Zhonghui Li, Zhoumin Li, Danke Xu
A visualized microarray sensing technique has been developed and applied to the screening of honey samples for residues of banned nitrofuran antibiotics. Using a multiplexed approach, metabolites of four main nitrofuran antibiotics can be detected simultaneously. Individual antigens were spotted onto 96-well plates. An indirective competitive assay format, with visualized signal response, was employed. An extraction method, based on derivatization with 2-nitrobenzaldehyde and partition into ethyl acetate, was used for screening...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27940240/advances-in-biosensor-development-for-the-screening-of-antibiotic-residues-in-food-products-of-animal-origin-a-comprehensive-review
#15
REVIEW
Valérie Gaudin
Antibiotic residues may be found in food of animal origin, since veterinary drugs are used for preventive and curative purposes to treat animals. The control of veterinary drug residues in food is necessary to ensure consumer safety. Screening methods are the first step in the control of antibiotic residues in food of animal origin. Conventional screening methods are based on different technologies, microbiological methods, immunological methods or physico-chemical methods (e.g. thin-layer chromatography, HPLC, LC-MS/MS)...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27879173/residue-depletion-of-oxytetracycline-otc-and-4-epi-oxytetracycline-4-epi-otc-in-broiler-chicken-s-claws-by-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms
#16
J Cornejo, E Pokrant, D Araya, C Briceño, H Hidalgo, A Maddaleno, C Araya-Jordán, B San Martin
Antibiotics are widely used in poultry production for the treatment of bacterial diseases. However, residues may remain in products and by-products destined for human consumption or animal feeding. The claws of chickens, which are a by-product of the poultry industry, can directly or indirectly enter the food chain as meals destined to feed other productive animals. Thus, it becomes necessary to determine and quantify antimicrobial residues present in this matrix. The objective of the study was to assess the depletion of oxytetracycline (OTC) and its metabolite 4-epi-OTC in broiler chicken's claws...
April 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27873208/validation-procedure-for-multiplex-antibiotic-immunoassays-using-flow-based-chemiluminescence-microarrays
#17
Verena Katharina Meyer, Daniela Meloni, Fabio Olivo, Erwin Märtlbauer, Richard Dietrich, Reinhard Niessner, Michael Seidel
Small molecules like antibiotics or other pharmaceuticals can be detected and quantified, among others, with indirect competitive immunoassays. With regard to multiplex quantification, these tests can be performed as chemiluminescence microarray immunoassays, in which, in principle, the analyte in the sample and the same substance immobilized on the chip surface compete for a limited number of specific antibody binding sites. The amount of the specific primary antibody that has been bound to the surface is visualized by means of a chemiluminescence reaction...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27857835/antibiotics-practice-and-opinions-of-cambodian-commercial-farmers-animal-feed-retailers-and-veterinarians
#18
Chhorvoin Om, Mary-Louise McLaws
BACKGROUND: Cambodia has reported multidrug resistant bacteria in poultry, similar to other countries in the region. We visited commercial food animal farms to explore opinions and antibiotic practices on the farms. METHODS: We used individual in-depth qualitative interviews with 16 commercial farmers, four feed retailers and nine veterinarians from food animal industry and government offices from the southwestern region of Phnom Penh. Transcribed interviews were thematically analysed...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27826358/use-of-molecular-typing-to-investigate-bacterial-translocation-from-the-intestinal-tract-of-chlorpyrifos-exposed-rats
#19
Claire Joly Condette, Bertin Elion Dzon, Farida Hamdad, Maurice Biendo, Véronique Bach, Hafida Khorsi-Cauet
BACKGROUND: Human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. Although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (BT). Previous studies have revealed that chlorpyrifos (CPF) exposure is associated with intestinal dysbiosis and the contamination of sterile organs...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27816225/multiresidue-analysis-of-cephalosporin-antibiotics-in-bovine-milk-based-on-molecularly-imprinted-polymer-extraction-followed-by-liquid-chromatography-tandem-mass-spectrometry
#20
A N Baeza, J L Urraca, R Chamorro, G Orellana, M Castellari, M C Moreno-Bondi
This work reports the preparation of molecularly imprinted polymers (MIPs) selective to cephalosporin (CF) antibiotics, and their application as molecularly imprinted solid-phase extraction (MISPE) sorbents for the determination of these antimicrobials in milk samples. Several functional monomers and cross-linkers have been screened to select the best combination that provides high selectivity for the simultaneous multiresidue extraction of cefthiofur (THIO), cefazolin (AZO), cefquinome (QUI), cephapirin (API), cephalexin (ALE) and cephalonium (ALO) from the samples...
November 25, 2016: Journal of Chromatography. A
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