Liu Ying, Zhu Zu-An, Liu Qing-Hua, Kong Qing-Yan, Liu Lei, Cui Tao, Wu Yong-Ping
To investigate the role of RAD001 in the reversing of drug resistance of SGC7901/DDP, we cultured SGC7901/DDP cells with different groups of drugs (RAD001, cisplatin (DDP) alone, or the combination of RAD001 and DDP); after that, we detected the drug sensitivity, cell apoptosis, and levels of P-gp, MRP1, and survivin in the cells of SGC7901/DDP by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphe-nyltetrazolium bromide) assay, flow cytometry, immunohistochemical analysis, and Western blot analysis. There was no significant difference between DDP 2...
September 2014: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine