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https://www.readbyqxmd.com/read/28098196/enhanced-removal-of-as-v-from-aqueous-solution-using-modified-hydrous-ferric-oxide-nanoparticles
#1
Lijuan Huo, Xibai Zeng, Shiming Su, Lingyu Bai, Yanan Wang
Hydrous ferric oxide (HFO) is most effective with high treatment capacity on arsenate [As(V)] sorption although its transformation and aggregation nature need further improvement. Here, HFO nanoparticles with carboxymethyl cellulose (CMC) or starch as modifier was synthesized for the purpose of stability improvement and As(V) removal from water. Comparatively, CMC might be the optimum stabilizer for HFO nanoparticles because of more effective physical and chemical stability. The large-pore structure, high surface specific area, and the non-aggregated nature of CMC-HFO lead to increased adsorption sites, and thus high adsorption capacities of As(V) without pre-treatment (355 mg·g(-1)), which is much greater than those reported in previous studies...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#2
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28094878/characterization-of-cellulose-degrading-bacteria-from-the-larval-gut-of-the-white-grub-beetle-lepidiota-mansueta-coleoptera-scarabaeidae
#3
Gautam Handique, Amrita Phukan, Badal Bhattacharyya, Abu Adil Lutful Haque Baruah, Syed Wasifur Rahman, Rajen Baruah
The goal of this study is to identify and characterize the cellulose degrading microorganisms in the larval gut of the white grub beetle, Lepidiota mansueta. Thirty bacterial strains were isolated and tested for cellulolytic activity using soluble carboxymethyl cellulose (CMC) degrading assays. Of these strains, five (FGB1, FB2, MB1, MB2, and HB1) degrade cellulose. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The highest cellulolytic index (2...
January 17, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28093922/toll-like-receptors-and-their-soluble-forms-differ-in-the-knee-and-thumb-basal-osteoarthritic-joints
#4
Goncalo Barreto, Jerker Sandelin, Abdelhakim Salem, Dan C Nordström, Eero Waris
Background and purpose - Although the pathogenesis of osteoarthritis (OA) is not well understood, chondrocyte-mediated inflammatory responses (triggered by the activation of innate immune receptors by damage-associated molecules) are thought to be involved. We examined the relationship between Toll-like receptors (TLRs) and OA in cartilage from 2 joints differing in size and mechanical loading: the first carpometacarpal (CMC-I) and the knee. Patients and methods - Samples of human cartilage obtained from OA CMC-I and knee joints were immunostained for TLRs (1-9) and analyzed using histomorphometry and principal component analysis (PCA)...
January 17, 2017: Acta Orthopaedica
https://www.readbyqxmd.com/read/28093324/synthesis-characterization-and-in-vitro-antitumor-activity-of-the-polyethylene-glycol-350-and-1000-succinate-derivatives-of-the-tocopherol-and-tocotrienol-isomers-of-vitamin-e
#5
Ahmed Abu-Fayyad, Sami Nazzal
Vitamin E refers to a group of saturated tocopherol (T) isomers and the biologically more active unsaturated tocotrienol (T3) isomers. PEGylated α-tocopherol, commercially known as Vitamin E TPGS, has been used as an emulsifier and therapeutic agent for children with vitamin E deficiency. Limited information, however, is available about the PEG conjugates of the tocotrienol isomers of vitamin E. The current work was therefore undertaken to synthesize and characterize the water soluble polyethylene glycol (PEG 350 and 1000) derivatives of T and T3...
January 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28091671/developing-the-family-of-picolinate-ligands-for-mn-2-complexation
#6
Attila Forgács, Rosa Pujales-Paradela, Martín Regueiro-Figueroa, Laura Valencia, David Esteban-Gómez, Mauro Botta, Carlos Platas-Iglesias
We have reported here a series of ligands containing pentadentate 6,6'-(azanediylbis(methylene))dipicolinic acid units that differ in the substituent present at the amine nitrogen atom (acetate: H3DPAAA; phenyl: H2DPAPhA; dodecyl: H2DPAC12A; 4-hexylphenyl: H2DPAC6PhA). The protonation constants of the hexadentate DPAAA(3-) and pentadentate DPAPhA(2-) ligands and the stability constants of their Mn(2+) complexes were determined using pH-potentiometry (25 °C, 0.15 M NaCl). The mono-hydrated [Mn(DPAAA)](-) complex (log KMnL = 13...
January 16, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28090315/chronic-mucocutaneous-candidiasis-disease-associated-with-inborn-errors-of-il-17-immunity
#7
REVIEW
Satoshi Okada, Anne Puel, Jean-Laurent Casanova, Masao Kobayashi
Chronic mucocutaneous candidiasis (CMC) is characterized by recurrent or persistent infections affecting the nails, skin and oral and genital mucosae caused by Candida spp., mainly Candida albicans. CMC is an infectious phenotype in patients with inherited or acquired T-cell deficiency. Patients with autosomal-dominant (AD) hyper IgE syndrome (HIES), AD signal transducer and activator of transcription 1 (STAT1) gain-of-function, autosomal-recessive (AR) deficiencies in interleukin (IL)-12 receptor β1 (IL-12Rβ1), IL-12p40, caspase recruitment domain-containing protein 9 (CARD9) or retinoic acid-related orphan receptor γT (RORγT) or AR autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) develop CMC as a major infectious phenotype that is categorized as Syndromic CMC...
December 2016: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/28088916/an-increasing-threat-in-an-iranian-referral-children-s-hospital-multidrug-resistant-acinetobacter-baumannii
#8
Setareh Mamishi, Reza Habibi, Babak Pourakbari, Shima Mahmoudi, Mohammad Taghi Haghi Ashtiani, Reihaneh Hosseinpour Sadeghi, Mahmoud Khodabandeh
INTRODUCTION: Acinetobacter baumannii has emerged as an important nosocomial pathogen in the past decades. Due to the prevalence of A. baumannii across the world, suitable typing methods to investigate the epidemiological distribution of the organism have been developed. The aim of this study was to investigate the epidemiological and molecular diversity of A. baumannii strains isolated from nosocomial infections of hospitalized children in Children Medical Center Hospital (CMC), an Iranian referral hospital, in Tehran, Iran...
January 13, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#9
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Matheus Augusto R N da Silva, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28088494/the-n-butyl-alcohol-extract-from-hibiscus-rosa-sinensis-l-flowers-enhances-healing-potential-on-rat-excisional-wounds
#10
Hui-Min Shen, Chun Chen, Ji-Yang Jiang, Yi-Lin Zheng, Wen-Feng Cai, Bin Wang, Zhen Ling, Liu Tang, Yuan-Hang Wang, Gang-Gang Shi
ETHNO-PHARMACOLOGICAL RELEVANCE: Hibiscus rosa-sinensis L. (HRS), a folk medicine named Zhujin in China, possess anti-tumor, antioxidant, antibacterial, low density lipoprotein oxidation prevention and macrophage death prevention effects. The leaves and red flowers of HRS have been traditionally used to treat with furuncle and ulceration. AIM OF THE STUDY: To investigate the efficacy and possible mechanism of the N-butyl alcohol extract of HRS (NHRS) red flowers in wound healing by analyzing the collagen fiber deposition, angiogenic activity and macrophages action of the NHRS...
January 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28081499/screening-active-compounds-from-corydalis-yanhusuo-by-combining-high-expression-vegf-receptor-hek293-cell-membrane-chromatography-with-hplc-esi-it-tof-msn-method
#11
Fen Wei, Qi Hu, Jing Huang, Shengli Han, Sicen Wang
Corydalis Thizoma,or Yuanhu in China, is a common herbal drug used for thousands of years as analgesic in Chinese medicine that has been reported to have potential anti-angiogenic effects. In this study, a VEGFR/cell membrane chromatography (VEGFR/CMC) coupled with HPLC- ESI-IT-TOF-MSn system was developed and successfully applied for identifying active components from YuanHu extract acting on VEGFR. We identified tetrahydropalmatine and corydaline as bioactive components with VEGFR activity, thus confirming their inhibitory activity on VEGFR engineered HEK293 cell growth by MTT assay...
January 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28079165/oriented-and-ordered-biomimetic-remineralization-of-the-surface-of-demineralized-dental-enamel-using-hap-acp-nanoparticles-guided-by-glycine
#12
Haorong Wang, Zuohui Xiao, Jie Yang, Danyang Lu, Anil Kishen, Yanqiu Li, Zhen Chen, Kehua Que, Qian Zhang, Xuliang Deng, Xiaoping Yang, Qing Cai, Ning Chen, Changhong Cong, Binbin Guan, Ting Li, Xu Zhang
Achieving oriented and ordered remineralization on the surface of demineralized dental enamel, thereby restoring the satisfactory mechanical properties approaching those of sound enamel, is still a challenge for dentists. To mimic the natural biomineralization approach for enamel remineralization, the biological process of enamel development proteins, such as amelogenin, was simulated in this study. In this work, carboxymethyl chitosan (CMC) conjugated with alendronate (ALN) was applied to stabilize amorphous calcium phosphate (ACP) to form CMC/ACP nanoparticles...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28074649/bio-based-artificial-nacre-with-excellent-mechanical-and-barrier-properties-realized-by-a-facile-in-situ-reduction-and-cross-linking-reaction
#13
Kiran Shahzadi, Imran Mohsin, Lin Wu, Xuesong Ge, Yijun Jiang, Hui Li, Xindong Mu
Demands for high strength integrated materials have substantially increased across various kinds of industries. Inspired by the relationship of excellent integration of mechanical properties and hierarchical nano/microscale structure of the natural nacre, a simple and facile method to fabricate high strength integrated artificial nacre based on sodium carboxymethylcellulose (CMC) and borate cross-linked graphene oxide (GO) sheets has been developed. The tensile strength and toughness of cellulose-based hybrid material reached 480...
January 11, 2017: ACS Nano
https://www.readbyqxmd.com/read/28074108/cellulolytic-robolector-towards-an-automated-high-throughput-screening-platform-for-recombinant-cellulase-expression
#14
Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur, Jochen Büchs
BACKGROUND: Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28073053/applying-3d-measurements-and-computer-matching-algorithms-to-two-firearm-examination-proficiency-tests
#15
Daniel Ott, Robert Thompson, Junfeng Song
In order for a crime laboratory to assess a firearms examiner's training, skills, experience, and aptitude, it is necessary for the examiner to participate in proficiency testing. As computer algorithms for comparisons of pattern evidence become more prevalent, it is of interest to test algorithm performance as well, using these same proficiency examinations. This article demonstrates the use of the Congruent Matching Cell (CMC) algorithm to compare 3D topography measurements of breech face impressions and firing pin impressions from a previously distributed firearms proficiency test...
December 21, 2016: Forensic Science International
https://www.readbyqxmd.com/read/28064052/development-of-novel-carboxymethyl-cellulose-k-carrageenan-blends-as-an-enteric-delivery-vehicle-for-probiotic-bacteria
#16
Alireza Dafe, Hossein Etemadi, Habib Zarredar, Gholam Reza Mahdavinia
This study reports a novel carrier based on blends of carboxymethyl cellulose (CMC) and k-Carrageenan (k-Carr) for probiotic colon delivery. Lactobacillus plantarum ATCC:13643 (L. plantarum) cells were encapsulated in CMC/k-Carr blends by extrusion method. k-carrageenan was used as a coating agent to improve encapsulation of L. plantarum cells in carboxymethyl cellulose biopolymer. K-Carrageenan and carboxymethyl cellulose were ionically cross-linked with K(+) and Ca(2+) ions, respectively. Optical and scanning electron microscopy obviously showed the random distribution of L...
January 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28060709/the-enhancement-of-nasal-drug-absorption-from-powder-formulations-by-the-addition-of-sodium-carboxymethyl-cellulose
#17
Akiko Tanaka, Tomoyuki Furubayashi, Hitomi Yamasaki, Katsuko Takano, Mayuko Kawakami, Shunsuke Kimura, Daisuke Inoue, Hidemasa Katsumi, Toshiyasu Sakane, Akira Yamamoto
For nasal drug absorption, powder formulations can be expected to provide many advantages. The first aim of this study was to examine drug absorption following nasal administration of powder formulations in rats. Pharmaceutical excipients are typically added to most powder formulations. The second aim was to investigate the change in nasal drug absorption of powder formulations in the presence of sodium carboxymethyl cellulose (CMC-Na). Model drugs used were norfloxacin (NFX), warfarin (WF), and piroxicam (PXC)...
December 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28058637/efficient-screening-of-potential-cellulases-and-hemicellulases-produced-by-bosea-sp-fbzp-16-using-the-combination-of-enzyme-assays-and-genome-analysis
#18
Aicha Asma Houfani, Tomáš Větrovský, Petr Baldrian, Said Benallaoua
Identification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28054589/large-scale-thick-self-assembled-nacre-mimetic-brick-walls-as-fire-barrier-coatings-on-textiles
#19
Paramita Das, Helga Thomas, Martin Moeller, Andreas Walther
Highly loaded polymer/clay nanocomposites with layered structures are emerging as robust fire retardant surface coatings. However, time-intensive sequential deposition processes, e.g. layer-by-layer strategies, hinders obtaining large coating thicknesses and complicates an implementation into existing technologies. Here, we demonstrate a single-step, water-borne approach to prepare thick, self-assembling, hybrid fire barrier coatings of sodium carboxymethyl cellulose (CMC)/montmorillonite (MTM) with well-defined, bioinspired brick-wall nanostructure, and showcase their application on textile...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28053882/hepatoprotective-potential-of-ethanolic-extract-of-aquilaria-agallocha-leaves-against-paracetamol-induced-hepatotoxicity-in-sd-rats
#20
Janey Alam, Md Mujahid, Badruddeen, Yasmeen Jahan, Paramdeep Bagga, Md Azizur Rahman
Many traditional systems of medicines employ herbal drugs for the hepatoprotection. Aim of the study was designed to evaluate the hepatoprotective potential of 'ethanolic extract of Aquilaria agallocha ( Chen Xiang) leaves' (AAE) against paracetamol (PCM) induced hepatotoxicity in SD rats. Group I animals were treated with 1% CMC for 8 days. Group II, III, IV and V animals were first treated with '1% CMC' 1 ml/kg/day, AAE 200 mg/kg/day, AAE 400 mg/kg/day and silymarin 100 mg/kg/day respectively for 7 days and then, orally administered with PCM 3 g/kg b...
January 2017: Journal of Traditional and Complementary Medicine
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