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Aleksandra Uruska, Dorota Zozulinska-Ziolkiewicz, Pawel Niedzwiecki, Monika Pietrzak, Bogna Wierusz-Wysocka
BACKGROUND: Insulin resistance (IR) is an important clinical issue in patients with type 1 diabetes due to worse metabolic control and risk of development of chronic complications. OBJECTIVE: The aim of the study was to evaluate simple and easily available parameters as indirect markers of IR in adults with type 1 diabetes and correlate it with the results of hyperinsulinemic-euglycemic clamp. METHODS: The study included 88 patients (62 men), aged 34...
January 31, 2018: Journal of Clinical Lipidology
Ewa Dybkowska, Anna Sadowska, Franciszek Świderski, Rita Rakowska, Katarzyna Wysocka
Over recent years, there has been increasing interest noted in those active substances derived from plants that show potential for preventing cancer development. The most promising candidate is resveratrol which can be found in large amounts in the skin of grapes, tomatoes and in red wine. Its beneficial effects on the human body are seen both in prevention and therapy. The anti-carcinogenic action of resveratrol is linked with its ability to neutralise reactive oxygen species and to modulate cellular processes such as apoptosis, and both cancerous cell proliferation and differentiation...
2018: Roczniki Państwowego Zakładu Higieny
Małgorzata Mackiewicz-Wysocka, Patrycja Czerwińska, Violetta Filas, Elżbieta Bogajewska, Agata Kubicka, Anna Przybyła, Ewelina Dondajewska, Tomasz Kolenda, Andrzej Marszałek, Andrzej Mackiewicz
Introduction: Twenty-five - fifty percent of skin melanomas arise from nevi. Melanocyte proliferation is activated by BRAF V600E , then is arrested, but single nevi transform to melanomas. p16 controls arrest, and p16 loss may promote transformation. Aim: To analyze BRAF V600E , p16 expression and melanocyte proliferation in dermal, compound and dysplastic nevi, cells of primary and metastatic melanoma in the Polish population. Material and methods: One hundred and thirty-two nevi (dermal, compound, dysplastic) and 41 melanomas ( in situ , primary, metastatic) were studied...
October 2017: Postȩpy Dermatologii i Alergologii
Agnieszka Szypowska, Anna Ramotowska, Marta Wysocka-Mincewicz, Artur Mazur, Lucyna Lisowicz, Iwona Beń-Skowronek, Joanna Sieniawska, Bożenna Klonowska, Dorota Charemska, Jolanta Nawrotek, Irena Jałowiec, Artur Bossowski, Milena Jamiołkowska, Beata Pyrżak, Grażyna Miszkurka, Mieczysław Szalecki
AIM: The seasonal variation of incidence of type 1 diabetes (T1D) theory supports the hypothesis that environmental factors play a role in the onset of the disease. The aim of this study is to assess seasonality of month of diagnosis in children with T1D in Poland. MATERIAL AND METHODS: the study group consisted of 2174 children from eastern and central Poland diagnosed with T1D between 2010 and 2014. Analysis was performed in different age groups, based on place of residence (rural/urban area) and depending on sex...
March 5, 2018: Experimental and Clinical Endocrinology & Diabetes
Andrzej Grzybowski, Waldemar Elikowski, Magdalena Gaca-Wysocka
RATIONALE: To analyze cardiovascular risk factors and comorbidity of acute unilateral visual loss due to combined central retinal vein occlusion (CRVO) and cilioretinal artery occlusion (CLRAO). PATIENT CONCERNS: Among patients with retinal vein or artery occlusion hospitalized at the Department of Ophthalmology between January 2011 and August 2017, subjects with combined CRVO/CLRAO were selected. All of them underwent ophthalmologic and cardiologic examination, including fluorescein angiography, optical coherence tomography, 12-lead electrocardiogram, transthoracic and transesophageal echocardiography, carotid Doppler sonography, cerebral magnetic resonance imaging, and a panel of laboratory tests...
January 2018: Medicine (Baltimore)
Jian Yan, Shi-An A Chen, Andrea Local, Tristin Liu, Yunjiang Qiu, Kristel M Dorighi, Sebastian Preissl, Chloe M Rivera, Chaochen Wang, Zhen Ye, Kai Ge, Ming Hu, Joanna Wysocka, Bing Ren
This corrects the article DOI: 10.1038/cr.2018.1.
March 2018: Cell Research
Peter Claes, Jasmien Roosenboom, Julie D White, Tomek Swigut, Dzemila Sero, Jiarui Li, Myoung Keun Lee, Arslan Zaidi, Brooke C Mattern, Corey Liebowitz, Laurel Pearson, Tomás González, Elizabeth J Leslie, Jenna C Carlson, Ekaterina Orlova, Paul Suetens, Dirk Vandermeulen, Eleanor Feingold, Mary L Marazita, John R Shaffer, Joanna Wysocka, Mark D Shriver, Seth M Weinberg
Genome-wide association scans of complex multipartite traits like the human face typically use preselected phenotypic measures. Here we report a data-driven approach to phenotyping facial shape at multiple levels of organization, allowing for an open-ended description of facial variation while preserving statistical power. In a sample of 2,329 persons of European ancestry, we identified 38 loci, 15 of which replicated in an independent European sample (n = 1,719). Four loci were completely new. For the others, additional support (n = 9) or pleiotropic effects (n = 2) were found in the literature, but the results reported here were further refined...
February 19, 2018: Nature Genetics
Marta Bartel, Barbara Wysocka, Pamela Krug, Daria Kępińska, Krystyna Kijewska, Gary J Blanchard, Katarzyna Kaczyńska, Katarzyna Lubelska, Katarzyna Wiktorska, Paulina Głowala, Marcin Wilczek, Marcin Pisarek, Jacek Szczytko, Andrzej Twardowski, Maciej Mazur
Fabrication of multifunctional smart vehicles for drug delivery is a fascinating challenge of multidisciplinary research at the crossroads of materials science, physics and biology. We demonstrate a prototypical microcapsule system that is capable of encapsulating hydrophobic molecules and at the same time reveals magnetic properties. The microcapsules are prepared using a templated synthesis approach where the molecules to be encapsulated (Nile Red) are present in the organic droplets that are suspended in the polymerization solution which also contains magnetic nanoparticles...
January 29, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Andrzej Grzybowski, Ronald A Schachar, Magdalena Gaca-Wysocka, Ira H Schachar, Barbara K Pierscionek
Purpose: To measure the maximum, objectively measured, accommodative amplitude, produced by pharmacologic stimulation. Methods: Thirty-seven healthy subjects were enrolled, with a mean age of 20.2±1.1 years, corrected visual acuity of 20/20, and mean spherical equivalent refraction (SER) =-0.83±1.60 diopters. For each subject, the right pupil was dilated with phenylephrine 10%. After 30 minutes, the pupil was measured, the left eye was patched, and the right eye was autorefracted...
2018: Clinical Ophthalmology
Bo Gu, Tomek Swigut, Andrew Spencley, Matthew R Bauer, Mingyu Chung, Tobias Meyer, Joanna Wysocka
To achieve guide RNA (gRNA) multiplexing and an efficient delivery of tens of distinct gRNAs into single cells, we develop a molecular assembly strategy, chimeric array of gRNA oligo (CARGO). We couple CARGO with dCas9 imaging to quantitatively measure movement of enhancers and promoters that undergo differentiation-associated activity changes in live embryonic stem cells. While all examined functional elements exhibit sub-diffusive behavior, their relative mobility increases concurrently with transcriptional activation...
January 25, 2018: Science
Eliezer Calo, Bo Gu, Margot E Bowen, Fardin Aryan, Antoine Zalc, Jialiang Liang, Ryan A Flynn, Tomek Swigut, Howard Y Chang, Laura D Attardi, Joanna Wysocka
Many craniofacial disorders are caused by heterozygous mutations in general regulators of housekeeping cellular functions such as transcription or ribosome biogenesis. Although it is understood that many of these malformations are a consequence of defects in cranial neural crest cells, a cell type that gives rise to most of the facial structures during embryogenesis, the mechanism underlying cell-type selectivity of these defects remains largely unknown. By exploring molecular functions of DDX21, a DEAD-box RNA helicase involved in control of both RNA polymerase (Pol) I- and II-dependent transcriptional arms of ribosome biogenesis, we uncovered a previously unappreciated mechanism linking nucleolar dysfunction, ribosomal DNA (rDNA) damage, and craniofacial malformations...
February 1, 2018: Nature
Izabela Wysocka, Ewa Kowalska, Konrad Trzciński, Marcin Łapiński, Grzegorz Nowaczyk, Anna Zielińska-Jurek
The combination of TiO₂ photocatalyst and magnetic oxide nanoparticles enhances the separation and recoverable properties of nanosized TiO₂ photocatalyst. Metal-modified (Me = Pd, Au, Pt, Cu) TiO₂/SiO₂@Fe₃O₄ nanocomposites were prepared by an ultrasonic-assisted sol-gel method. All prepared samples were characterized by X-ray powder diffraction (XRD) analysis, Brunauer-Emmett-Teller (BET) method, X-ray photoelectron spectroscopy (XPS), scanning transmission electron microscopy (STEM), Mott-Schottky analysis and photoluminescence spectroscopy (PL)...
January 7, 2018: Nanomaterials
Jian Yan, Shi-An A Chen, Andrea Local, Tristin Liu, Yunjiang Qiu, Kristel M Dorighi, Sebastian Preissl, Chloe M Rivera, Chaochen Wang, Zhen Ye, Kai Ge, Ming Hu, Joanna Wysocka, Bing Ren
Long-range chromatin interactions between enhancers and promoters are essential for transcription of many developmentally controlled genes in mammals and other metazoans. Currently, the exact mechanisms that connect distal enhancers to their specific target promoters remain to be fully elucidated. Here, we show that the enhancer-specific histone H3 lysine 4 monomethylation (H3K4me1) and the histone methyltransferases MLL3 and MLL4 (MLL3/4) play an active role in this process. We demonstrate that in differentiating mouse embryonic stem cells, MLL3/4-dependent deposition of H3K4me1 at enhancers correlates with increased levels of chromatin interactions, whereas loss of this histone modification leads to reduced levels of chromatin interactions and defects in gene activation during differentiation...
January 9, 2018: Cell Research
Irena Wysocka, Emilia Vassileva
This article describes the development and validation of an analytical procedure for the matrix separation, preconcentration and determination of sub-ng kg-1 levels 232Th in a small volume (20 mL) of seawater samples. The matrix separation and Th preconcentration was carried out using a commercially available ion-chelation system seaFAST-pico. The acidified to pH < 2 seawater samples were mixed on-line with the ammonium acetate buffer (pH of 6.0 ± 0.2) before loading on the column containing resin with iminodiacetic and ethylenediaminetriacetic functional groups...
February 13, 2018: Analytica Chimica Acta
Marta Baszyńska-Wilk, Marta Wysocka-Mincewicz, Anna Świercz, Jolanta Świderska, Magdalena Marszał, Mieczysław Szalecki
BACKGROUND: Neurological complications of diabetic ketoacidosis are considered to be very serious clinical problem. The most common complication is cerebral edema. However this group includes also less common syndromes such as ischemic or hemorrhagic stroke, cerebral venous and sinus thrombosis or very rare peripheral neuropathy. CASE REPORT: We present a case of 9-year old girl with new onset type 1 diabetes, diabetic ketoacidosis, cerebral edema, multifocal vasogenic brain lesions and lower limbs peripheral paresis...
December 8, 2017: Journal of Clinical Research in Pediatric Endocrinology
Agnieszka Siebert, Magdalena Wysocka, Beata Krawczyk, Grzegorz Cholewiński, Janusz Rachoń
The series of 16 novel amino acid and peptide mycophenolic acid (MPA) derivatives was obtained as potential antibacterial agents. Coupling of MPA with respective amines was optimized with condensing reagents such as EDCI/DMAP and T3P/TEA. Amino acid analogs were received both as methyl esters and also with the free carboxylic group. The biological activity of the products was tested on five references bacterial strains: Klebsiella pneumoniae ATCC 700603 (ESBL), Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus MRSA ATCC 43300, Staphylococcus aureus MSSA ATCC 25923...
January 1, 2018: European Journal of Medicinal Chemistry
Nian Liu, Cameron H Lee, Tomek Swigut, Edward Grow, Bo Gu, Michael C Bassik, Joanna Wysocka
Transposable elements, also known as transposons, are now recognized not only as parasitic DNA, the spread of which in the genome must be controlled by the host, but also as major players in genome evolution and regulation. Long interspersed element-1 (LINE-1, also known as L1), the only currently autonomous mobile transposon in humans, occupies 17% of the genome and generates inter- and intra-individual genetic variation, in some cases resulting in disease. However, how L1 activity is controlled and the function of L1s in host gene regulation are not completely understood...
January 11, 2018: Nature
Hironobu Okuno, Francois Renault Mihara, Shigeki Ohta, Kimiko Fukuda, Kenji Kurosawa, Wado Akamatsu, Tsukasa Sanosaka, Jun Kohyama, Kanehiro Hayashi, Kazunori Nakajima, Takao Takahashi, Joanna Wysocka, Kenjiro Kosaki, Hideyuki Okano
CHARGE syndrome is caused by heterozygous mutations in the chromatin remodeler, CHD7, and is characterized by a set of malformations that, on clinical grounds, were historically postulated to arise from defects in neural crest formation during embryogenesis. To better delineate neural crest defects in CHARGE syndrome, we generated induced pluripotent stem cells (iPSCs) from two patients with typical syndrome manifestations, and characterized neural crest cells differentiated in vitro from these iPSCs (iPSC-NCCs)...
November 28, 2017: ELife
Andrzej Grzybowski, Ronald A Schachar, Magdalena Gaca-Wysocka, Ira H Schachar, Farhad Kamangar, Barbara K Pierscionek
PURPOSE: Our purpose was to determine the changes in anterior chamber depth (ACD) and central lens thickness (CLT) during pharmacologically induced accommodation. METHODS: Following pupillary dilation with phenylephrine 10%, baseline auto-refractions and swept-source optical coherence tomographic biometric images (Zeiss IOLMaster 700) were obtained from the right eyes of 25 subjects aged 19 to 24 years. Pilocarpine 4% and phenylephrine 10% were then instilled into these right eyes...
November 16, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
Mieczysław Szalecki, Marta Wysocka-Mincewicz, Anna Ramotowska, Artur Mazur, Lucyna Lisowicz, Iwona Ben-Skowronek, Joanna Sieniawska, Bożena Klonowska, Dorota Charemska, Jolanta Nawrotek, Irena Jałowiec, Artur Bossowski, Milena Jamiołkowska, Beata Pyrżak, Grażyna Miszkurka, Agnieszka Szypowska
BACKGROUND: The incidence of childhood Type-1 diabetes (T1D) varies greatly between populations and the estimates and/or predictions of the rates would aid in adequate planning of health care resources. The study's aim was to assess the incidence of T1D in the paediatric population of eastern and central Poland. METHODS: In this cohort study covering the period from January 2010 to December 2014 data were collected for children and adolescents below 18 years of age with newly diagnosed T1D, living in eastern and central Poland...
November 16, 2017: Diabetes/metabolism Research and Reviews
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