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3D biocompatibility

Noam Cohen, Einat Toister, Yonatan Lati, Meni Girshengorn, Lilach Levin, Lea Silberstein, Dror Seliktar, Eyal Epstein
Recent advances in the bioengineering field have introduced new opportunities enabling cell encapsulation in three-dimensional (3D) structures using either various natural or synthetic materials. However, such hydrogel scaffolds have not been fully biocompatible for cell cultivation due to the lack of physical stability or bioactivity. Here, we utilized a uniquely fabricated semi-synthetic 3D polyethylene glycol-fibrinogen (PEG-Fb) hydrogel scaffold, which exhibits both high stability and high bioactivity, to encapsulate HEK293 cells for the production of human recombinant acetylcholine esterase (AChE)...
February 21, 2018: Cytotechnology
Tobias Dahlberg, Tim Stangner, Hanqing Zhang, Krister Wiklund, Petter Lundberg, Ludvig Edman, Magnus Andersson
We report a novel method for fabrication of three-dimensional (3D) biocompatible micro-fluidic flow chambers in polydimethylsiloxane (PDMS) by 3D-printing water-soluble polyvinyl alcohol (PVA) filaments as master scaffolds. The scaffolds are first embedded in the PDMS and later residue-free dissolved in water leaving an inscription of the scaffolds in the hardened PDMS. We demonstrate the strength of our method using a regular, cheap 3D printer, and evaluate the inscription process and the channels micro-fluidic properties using image analysis and digital holographic microscopy...
February 20, 2018: Scientific Reports
Xuan Zhou, Haitao Cui, Margaret Nowicki, Shida Miao, Se-Jun Lee, Fahed Masood, Brent T Harris, Lijie Grace Zhang
Central nerve repair and regeneration remain challenging problems worldwide largely because of the extremely weak inherent regenerative capacity and accompanying fibrosis of native nerves. Inadequate solutions to unmet needs for clinical therapeutics encourage the development of novel strategies to promote nerve regeneration. Recently, 3D bioprinting techniques, as one of a set of valuable tissue engineering technologies, have shown great promise toward fabricating complex and customizable artificial tissue scaffolds...
February 20, 2018: ACS Applied Materials & Interfaces
Mamatha M Pillai, J Gopinathan, R Senthil Kumar, G Sathish Kumar, S Shanthakumari, K Santosh Sahanand, Amitava Bhattacharyya, R Selvakumar
Tissue engineered constructs with rapid restoration of mechanical and biological properties remains a challenge, emphasizing the need to develop novel scaffolds. Here we present a multi-component composite 3D scaffold structure with biomimetic reinforcement and biomolecule functionalization for meniscus tissue engineering. The scaffold structure was developed using 3:1 silk fibroin (SF) and polyvinyl alcohol (PVA). Autoclaved eggshell membrane (AESM) powder (1-3%w/v) was used as reinforcement to enhance biomechanical properties...
February 20, 2018: Journal of Biomedical Materials Research. Part A
Pan Tian, Wenwen Yi, Chaoyang Chen, Jie Hu, Jin Qi, Boshen Zhang, Mark Ming-Cheng Cheng
The cuff electrode provides a stable interface with peripheral nerves, which has been widely used in basic research and clinical practice. Currently, the cuff electrodes are limited by the planar processing of microfabrication. This paper presents a novel cuff electrode using high-aspect ratio carbon nanotubes (CNTs) integrated on a flexible biocompatible parylene. The microfabrication process unites the high quality vertical CNTs grown at high temperature with the heat sensitive parylene substrate in a highly controllable manner...
February 19, 2018: Biomedical Microdevices
Jonas C Rose, David B Gehlen, Tamás Haraszti, Jens Köhler, Christopher J Licht, Laura De Laporte
Natural healing is based on highly orchestrated processes, in which the extracellular matrix plays a key role. To resemble the native cell environment, we introduce an artificial extracellular matrix (aECM) with the capability to template hierarchical and anisotropic structures in situ, allowing a minimally-invasive application via injection. Synthetic, magnetically responsive, rod-shaped microgels are locally aligned and fixed by a biocompatible surrounding hydrogel, creating a hybrid anisotropic hydrogel (Anisogel), of which the physical, mechanical, and chemical properties can be tailored...
February 9, 2018: Biomaterials
Bedia Begum Karakocak, Jue Liang, Pratim Biswas, Nathan Ravi
For targeted delivery with nanoparticles (NPs) as drug carriers, it is imperative that the NPs are internalized into the targeted cell. Surface properties of NPs influence their interactions with cells. We examined the responses of retinal pigment epithelial cells, NIH 3T3 fibroblast cells, and Chinese hamster ovary cells to gold nanoparticles (Au NPs) in their nascent form as well as coated with end-thiolated hyaluronate (HS-HA). The grafting density of HS-HA on Au NPs was calculated based on total organic carbon measurements and thermal gravimetric analysis...
April 15, 2018: Carbohydrate Polymers
Chao Qi, Jia Liu, Yang Jin, Luming Xu, Guobin Wang, Zheng Wang, Lin Wang
Millions of patients worldwide suffer from cartilage injury and age/disease-related cartilage degeneration. However, cartilage, such as articular cartilage, is poor at self-regeneration. Current treatments are often invasive with limited efficacy. Developing minimal invasive strategies for effective cartilage repair is highly desired. Here, we report an injectable, photo-crosslinkable sericin hydrogel as a biomimetic extracellular matrix for minimal invasively repairing cartilage. Sericin was functionalized to be sericin methacryloyl (SerMA), which formed an in situ hydrogel upon UV light irradiation via photo-crosslinking...
February 9, 2018: Biomaterials
Zhouzhou Bao, Yuqing Zhang, Ziyang Tan, Xia Yin, Wen Di, Jian Ye
The sentinel lymph node (SLN) biopsy is gaining in popularity as a procedure to investigate the lymphatic metastasis of malignant tumors. The commonly used techniques to identify the SLNs in clinical practice are blue dyes-guided visualization, radioisotope-based detection and near-infrared fluorescence imaging. However, all these methods have not been found to perfectly fit the clinical criteria with issues such as short retention time in SLN, poor spatial resolution, autofluorescence, low photostability and high cost...
February 10, 2018: Biomaterials
Jakub Zimoch, Joan Simó Padial, Agnes S Klar, Queralt Vallmajo-Martin, Martin Meuli, Thomas Biedermann, Christopher J Wilson, Alan Rowan, Ernst Reichmann
Molecular and mechanical interactions with the 3D extracellular matrix are essential for cell functions such as survival, proliferation, migration, and differentiation. Thermo-responsive biomimetic polyisocyanopeptide (PIC) hydrogels are promising new candidates for 3D cell, tissue, and organ cultures. This is a synthetic, thermo-responsive and stress-stiffening material synthesized via polymerization of the corresponding monomers using a nickel perchlorate as a catalyst. It can be tailored to meet various demands of cells by modulating its stiffness and through the decoration of the polymer with short GRGDS peptides using copper free click chemistry...
February 14, 2018: Acta Biomaterialia
Wei Zhu, Haitao Cui, Benchaa Boualam, Fahed Masood, Erin Flynn, Raj Rao, Zhiyong Zhang, Lijie Grace Zhang
Cartilage tissue is prone to degradation and has little capacity for self-healing due to its avascularity. Tissue engineering, which provides artificial scaffolds to repair injured tissues, is a novel and promising strategy for cartilage repair. 3D bioprinting offers even greater potential for repairing degenerative tissue by simultaneously integrating living cells, biomaterials, and biological cues to provide a customized scaffold. With regard to cell selection, mesenchymal stem cells (MSCs) hold great capacity for differentiating into a variety of cell types, including chondrocytes, and could therefore be utilized as a cartilage cell source in 3D bioprinting...
February 15, 2018: Nanotechnology
Wei Yuan, Xin He, Xiaoshu Zhou, Yue Zhu
Hydroxyapatite nanoparticles were prepared by hydrothermal method using calcium nitrate and phosphoric acid as precursors and ammonia aqueous solution as a pH value adjustor. The 3D-printed porous Ti6Al4V and CoCrMo alloy scaffolds were effectively coated with hydroxyapatite nanoparticles in the hydrothermal synthesis process by deposition method. Coating hydroxyapatite nanoparticles on the implant surfaces increased their biocompatibility and bioactivity. HAP-deposited Ti6Al4V or HAP-deposited CoCrMo scaffold induced no statistical increase in terms of apoptosis in hFOB1...
June 1, 2018: Journal of Nanoscience and Nanotechnology
Monica A Serban, Aleksander Skardal
Hyaluronan is a ubiquitous constituent of mammalian extracellular matrices and, because of its excellent intrinsic biocompatibility and chemical modification versatility, has been widely employed in a multitude of biomedical applications. In this article, we will survey the approaches used to tailor hyaluronan to specific needs of tissue engineering, regenerative and reconstructive medicine and overall biomedical research. We will also describe recent examples of applications in these broader areas, such as 3D cell culture, bioprinting, organoid biofabrication, and precision medicine that are facilitated by the use of hyaluronan as a biomaterial...
February 10, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
A Batth, I Thompson
The requirement for improved modelling of cells in culture for study of cell-to-cell interactions has led to an increased focus on three-dimensional (3D) in vitro models. In this study, NG108-15 neural cells and rat sciatic nerve Schwann cells were successfully grown as monocultures and as a co-culture on the biocompatible nylon mesh substrates. This has allowed proliferation and interactions between the two cell types to be monitored on the mesh over time. Electrophysiological recordings validated the NG108-15 neural cell differentiation which confirmed the similarity of recorded action potentials in 2D culture environments...
February 12, 2018: Journal of Biomedical Materials Research. Part A
Leila Fathyunes, Jafar Khalil-Allafi
In the present work, we investigated the effect of employing ultrasonic waves during pulse electrochemical deposition on surface topography, chemical composition and biocompatibility of calcium phosphate (Ca-P) coatings. The SEM and 3D AFM images showed that the anodized titanium surface was covered with the uniform and refined size of plate-like Ca-P crystals, when the ultrasonic treatment of the electrolyte with power of 60 W was carried out during deposition. In contrast, for the Ca-P; 0 W coating applied under only the magnetic stirring of the electrolyte, the microstructure was non-uniform and some Ca-P crystals with the larger size were randomly observed in different regions, causing a rougher surface...
April 2018: Ultrasonics Sonochemistry
Yaimara Solìs Moré, Gloria Panella, Giulia Fioravanti, Francesco Perrozzi, Maurizio Passacantando, Francesco Giansanti, Matteo Ardini, Luca Ottaviano, Annamaria Cimini, Carlos Peniche, Rodolfo Ippoliti
Novel 2D films and 3D scaffolds based on chitosan (CHI), apatite (Ap) and graphene oxide (GO) were developed by an in situ synthesis in which self-assembly process was conducted to direct partial reduction of GO by chitosan in acidic medium. Physical-chemical characterization was carried out by optical microscopy (OM), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). In vitro biological studies using murine fibroblast (MC3T3) and human neuroblastoma (SH-SY5Y) cell lines were also performed...
February 9, 2018: Journal of Biomedical Materials Research. Part A
Hongbo Chen, Jiaming Sun, Jie-Cong Wang
PURPOSE: The objective of this article is to present the long-term outcomes of digital three-dimensional (3D) hydroxyapatite (HA) implants used for calvarial defects. Porous HA provide good results and have many characteristics, making it suitable for calvarial defect reconstruction. However, using HA may be associated with potentially serious complications, including infection and exposure. The authors used digital 3D HA for cranioplasty for many years and accumulated considerable experience from using it...
February 7, 2018: Journal of Craniofacial Surgery
Changlu Xu, Yanjie Bai, Shaofeng Yang, Huilin Yang, David A Stout, Phong A Tran, Lei Yang
The fabrication strategies of three-dimensional porous biomaterials have been extensively studied and well established in the past few decades, yet the biocompatibility and versatility of porous architecture preparation is still lacking. Herewith, we present a novel and green 3D porous foam fabrication technique for both soft and hard engineering. By utilizing the gelatinization and retrogradation properties of starches, stabilized porous constructs made of various building blocks, from living cells to ceramic particles, were created for the first time...
February 8, 2018: Biomedical Materials
Martin Koller
Polyhydroxyalkanoates (PHA) are bio-based microbial biopolyesters; their stiffness, elasticity, crystallinity and degradability are tunable by the monomeric composition, selection of microbial production strain, substrates, process parameters during production, and post-synthetic processing; they display biological alternatives for diverse technomers of petrochemical origin. This, together with the fact that their monomeric and oligomeric in vivo degradation products do not exert any toxic or elsewhere negative effect to living cells or tissue of humans or animals, makes them highly stimulating for various applications in the medical field...
February 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Heyong Yin, Zexing Yan, Richard Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa Docheva
Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons...
February 8, 2018: Biomedical Materials
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