keyword
MENU ▼
Read by QxMD icon Read
search

Autologous priming

keyword
https://www.readbyqxmd.com/read/27913524/cellular-and-vaccine-immunotherapy-for-multiple-myeloma
#1
Alfred L Garfall, Edward A Stadtmauer
Allogeneic hematopoietic cell transplantation and donor lymphocyte infusion for multiple myeloma (MM) can induce graft-versus-myeloma immunity and long-term survivorship, but limited efficacy and associated toxicities have prevented its widespread use. Cellular immunotherapies and vaccines seek to induce more specific, reliable, and potent antimyeloma immune responses with less treatment-related risk than is possible with allogeneic transplantation. Advances in molecular biology, and basic and applied immunology, have led to promising approaches such as genetically engineered T cells with chimeric antigen receptors and T-cell receptors targeting myeloma-specific epitopes, vaccine primed ex vivo expanded autologous T cells, expanded marrow-infiltrating lymphocytes, and plasma cell/dendritic cell fusion vaccines...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27874054/combinational-immunotherapy-with-allo-dribble-vaccines-and-anti-ox40-co-stimulation-leads-to-generation-of-cross-reactive-effector-t-cells-and-tumor-regression
#2
Guangjie Yu, Yuhuan Li, Zhihua Cui, Nicholas P Morris, Andrew D Weinberg, Bernard A Fox, Walter J Urba, Lixin Wang, Hong-Ming Hu
It is well-known that vaccines comprising of irradiated whole tumor cells or tumor-derived heat shock proteins can generate tumor-specific immune responses. In contrast, we showed recently that vaccines composed of autophagosomes (DRibbles) derived from syngeneic sarcomas could induce cross-reactive T-cell responses and cross-protection against the tumor. This unusual property of DRibbles was related to the selective recruitment of defective ribosomal products (DRiPs) and other short-lived proteins (SLiPs) into autophagosomes via sequestosome (SQSTM1, p62) mediated association of ubiquitinated SLiPs to the autophagy gene product LC3...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27867632/the-modest-outcome-of-clinical-trials-with-bone-marrow-cells-for-myocardial-repair-is-the-autologous-source-of-cells-the-prime-culprit
#3
COMMENT
Muhammad Siddique Shahid, Wael Lasheen, Khawaja Husnain Haider
No abstract text is available yet for this article.
October 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27863558/development-of-an-autologous-canine-cancer-vaccine-system-for-resectable-malignant-tumors-in-dogs
#4
J R Yannelli, R Wouda, T J Masterson, M G Avdiushko, D A Cohen
While conventional therapies exist for canine cancer, immunotherapies need to be further explored and applied to the canine setting. We have developed an autologous cancer vaccine (K9-ACV), which is available for all dogs with resectable disease. K9-ACV was evaluated for safety and immunogenicity for a variety of cancer types in a cohort of companion dogs under veterinary care. The autologous vaccine was prepared by enzymatic digestion of solid tumor biopsies. The resultant single cell suspensions were then UV-irradiated resulting in immunogenic cell death of the tumor cells...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27863082/allogeneic-platelet-rich-plasma-affects-monocyte-differentiation-to-dendritic-cells-causing-an-anti-inflammatory-microenvironment-putatively-fostering-the-wound-healing
#5
Andrea Papait, Ranieri Cancedda, Maddalena Mastrogiacomo, Alessandro Poggi
Autologous platelet rich plasma (PRP) is clinically used to induce repair of different tissues through the release of bioactive molecules. In some patients, the production of an efficient autologous PRP is unfeasible due to their compromised health. We developed an allogeneic PRP mismatched for AB0 and Rh antigens. To broadcast its clinical applications avoiding side effects the outcome of allogeneic PRP on immune response should be defined. Thus, we investigated whether PRP affected the differentiation of peripheral blood monocytes to dendritic cells upon stimulation with granulocyte monocyte colony stimulating factor and interleukin-4...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27846835/equine-dendritic-cells-generated-with-horse-serum-have-enhanced-functionality-in-comparison-to-dendritic-cells-generated-with-fetal-bovine-serum
#6
Anja Ziegler, Helen Everett, Eman Hamza, Mattia Garbani, Vinzenz Gerber, Eliane Marti, Falko Steinbach
BACKGROUND: Dendritic cells are professional antigen-presenting cells that play an essential role in the initiation and modulation of T cell responses. They have been studied widely for their potential clinical applications, but for clinical use to be successful, alternatives to xenogeneic substances like fetal bovine serum (FBS) in cell culture need to be found. Protocols for the generation of dendritic cells ex vivo from monocytes are well established for several species, including horses...
November 15, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27845055/is-stem-cell-therapy-ready-for-prime-time-in-treatment-of-inflammatory-bowel-diseases
#7
Christopher J Hawkey, Daniel W Hommes
Autologous hematopoietic stem cell transplantation (HSCT) and mesenchymal stromal cell therapy (MSCT) have been proposed for patients with refractory Crohn's disease (CD) and fistulizing CD respectively. Will these highly advanced techniques be available only for select patients, at specialized centers, or is further clinical development justified, with the aim of offering widespread, more definitive therapeutic options for often very difficult to treat disease? Patients with CD who are eligible for HSCT have typically been failed by most approved therapies, have undergone multiple surgeries, and have coped with years of disease activity and poor quality of life...
November 11, 2016: Gastroenterology
https://www.readbyqxmd.com/read/27812850/generation-of-t-cell-effectors-using-tumor-cell-loaded-dendritic-cells-for-adoptive-t-cell-therapy
#8
Katerina Vavrova, Petra Vrabcova, Dominik Filipp, Jirina Bartunkova, Rudolf Horvath
Adoptive T cell transfer has been shown to be an effective method used to boost tumor-specific immune responses in several types of malignancies. In this study, we set out to optimize the ACT protocol for the experimental treatment of prostate cancer. The protocol includes a pre-stimulation step whereby T cells were primed with autologous dendritic cells loaded with the high hydrostatic pressure-treated prostate cancer cell line, LNCaP. Primed T cells were further expanded in vitro with anti-CD3/CD28 Dynabeads in the WAVE bioreactor 2/10 system and tested for cytotoxicity...
December 2016: Medical Oncology
https://www.readbyqxmd.com/read/27741149/blood-conservation-strategies-in-cardiac-valve-replacement-a-retrospective-analysis-of-1645-patients
#9
Junnan Zheng, Liangwei Chen, Linfeng Qian, Jianjie Jiang, Yinglian Chen, Jue Xie, Liping Shi, Yiming Ni, Haige Zhao
We aimed to evaluate whether blood conservation strategies including intraoperative autologous donation (IAD) could reduce perioperative blood transfusion for patients undergoing cardiac valve replacement including mitral valve replacement, aortic valve replacement (AVR), and double valve replacement (DVR).A total of 726 patients were studied over a 3-year period (2011-2013) after the implementation of IAD and were compared with 919 patients during the previous 36-month period (January 2008-December 2010). The method of small-volume retrograde autologous priming, strict blood transfusion standard together with IAD constituted a progressive blood-saving strategy...
October 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27729702/blood-conservation-a-team-sport
#10
REVIEW
Donald S Likosky, Timothy A Dickinson, Theron A Paugh
Cardiac surgery accounts for between 15% and 20% of all blood product utilization in the United States. A body of literature suggests that patients who are exposed to even small quantities of blood have an increased risk of morbidity and mortality, even after adjusting for pre-operative risk. Despite this body of literature supporting a restrictive blood management strategy, wide variability in transfusion rates exist across institutions. Recent blood management guidelines have shed light on a number of potentially promising blood management strategies, including acute normovolemic hemodilution (ANH) and retrograde autologous priming (RAP)...
September 2016: Journal of Extra-corporeal Technology
https://www.readbyqxmd.com/read/27721864/cardiopulmonary-bypass-priming-using-autologous-cord-blood-in-neonatal-congenital-cardiac-surgery
#11
Eun Seok Choi, Sungkyu Cho, Woo Sung Jang, Woong-Han Kim
BACKGROUND AND OBJECTIVES: A blood transfusion is almost inevitable in neonatal cardiac surgery. This study aimed to assess the feasibility of using autologous cord blood for a cardiopulmonary bypass (CPB) priming as an alternative to an allo-transfusion in neonatal cardiac surgery. SUBJECTS AND METHODS: From January 2012 to December 2014, cord blood had been collected during delivery after informed consent and was stored immediately into a blood bank. Eight neonatal patients had their own cord blood used for CPB priming during cardiac surgery...
September 2016: Korean Circulation Journal
https://www.readbyqxmd.com/read/27705922/umbilical-cord-derived-mesenchymal-stem-cells-reversed-the-suppressive-deficiency-of-t-regulatory-cells-from-peripheral-blood-of-patients-with-multiple-sclerosis-in-a-co-culture-a-preliminary-study
#12
Hongna Yang, Jinhua Sun, Feng Wang, Yan Li, Jianzhong Bi, Tingyu Qu
The immunoregulatory function of T regulatory cells (Tregs) is impaired in multiple sclerosis (MS). Recent studies have shown that umbilical cord-derived mesenchymal stem cells (UC-MSCs) exert regulatory effect on the functions of immune cells. Thus, we investigated whether UC-MSCs could improve the impaired function of Tregs from MS patients. Co-cultures of UC-MSCs with PBMCs of MS patients were performed for 3 days. Flow cytometry was used to determine the frequency of Tregs. A cell proliferation assay was used to evaluate the suppressive capacity of Tregs...
September 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27698654/dexamethasone-and-monophosphoryl-lipid-a-modulated-dendritic-cells-promote-antigen-specific-tolerogenic-properties-on-naive-and-memory-cd4-t-cells
#13
Jaxaira Maggi, Katina Schinnerling, Bárbara Pesce, Catharien M Hilkens, Diego Catalán, Juan C Aguillón
Tolerogenic dendritic cells (DCs) are a promising tool to control T cell-mediated autoimmunity. Here, we evaluate the ability of dexamethasone-modulated and monophosphoryl lipid A (MPLA)-activated DCs [MPLA-tolerogenic DCs (tDCs)] to exert immunomodulatory effects on naive and memory CD4(+) T cells in an antigen-specific manner. For this purpose, MPLA-tDCs were loaded with purified protein derivative (PPD) as antigen and co-cultured with autologous naive or memory CD4(+) T cells. Lymphocytes were re-challenged with autologous PPD-pulsed mature DCs (mDCs), evaluating proliferation and cytokine production by flow cytometry...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27644879/osteoarthritis-derived-chondrocytes-are-a-potential-source-of-multipotent-progenitor-cells-for-cartilage-tissue-engineering
#14
Tomoyuki Oda, Tadahiro Sakai, Hideki Hiraiwa, Takashi Hamada, Yohei Ono, Motoshige Nakashima, Shinya Ishizuka, Tetsuya Matsukawa, Satoshi Yamashita, Saho Tsuchiya, Naoki Ishiguro
The natural healing capacity of damaged articular cartilage is poor, rendering joint surface injuries a prime target for regenerative medicine. While autologous chondrocyte or mesenchymal stem cell (MSC) implantation can be applied to repair cartilage defects in young patients, no appropriate long-lasting treatment alternative is available for elderly patients with osteoarthritis (OA). Multipotent progenitor cells are reported to present in adult human articular cartilage, with a preponderance in OA cartilage...
October 21, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27630232/fc-receptor-mediated-activities-of-env-specific-human-monoclonal-antibodies-generated-from-volunteers-receiving-the-dna-prime-protein-boost-hiv-vaccine-dp6-001
#15
Matthew R Costa, Justin Pollara, Regina Whitney Edwards, Michael S Seaman, Miroslaw K Gorny, David C Montefiori, Hua-Xin Liao, Guido Ferrari, Shan Lu, Shixia Wang
: HIV-1 is able to elicit broadly potent neutralizing antibodies in a very small subset of individuals only after several years of infection, and therefore, vaccines that elicit these types of antibodies have been difficult to design. The RV144 trial showed that moderate protection is possible and that this protection may correlate with antibody-dependent cellular cytotoxicity (ADCC) activity. Our previous studies demonstrated that in an HIV vaccine phase I trial, the DP6-001 trial, a polyvalent Env DNA prime-protein boost formulation could elicit potent and broadly reactive, gp120-specific antibodies with positive neutralization activities...
November 15, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27606351/prophylactic-and-therapeutic-adenoviral-vector-based-multivirus-specific-t-cell-immunotherapy-for-transplant-patients
#16
Vijayendra Dasari, Andrea Schuessler, Corey Smith, Yide Wong, John J Miles, Mark J Smyth, George Ambalathingal, Ross Francis, Scott Campbell, Daniel Chambers, Rajiv Khanna
Viral infections including cytomegalovirus, Epstein-Barr virus, adenovirus, and BK virus are a common and predictable problem in transplant recipients. While cellular immune therapies have been successfully used to tackle infectious complications in transplant recipients, manufacturing immunotherapies to address the multitude of possible pathogens can be technically challenging and labor-intensive. Here we describe a novel adenoviral antigen presentation platform (Ad-MvP) as a tool for rapid generation of multivirus-specific T-cells in a single step...
2016: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/27585815/hormonal-modulation-of-dendritic-cells-differentiation-maturation-and-function-implications-for-the-initiation-and-progress-of-systemic-autoimmunity
#17
Juan Pablo Mackern-Oberti, Evelyn L Jara, Claudia A Riedel, Alexis M Kalergis
Hormonal homeostasis is crucial for keeping a competent and healthy immune function. Several hormones can modulate the function of various immune cells such as dendritic cells (DCs) by influencing the initiation of the immune response and the maintenance of peripheral tolerance to self-antigens. Hormones, such as estrogens, prolactin, progesterone and glucocorticoids may profoundly affect DCs differentiation, maturation and function leading to either a pro-inflammatory or an anti-inflammatory (or tolerogenic) phenotype...
September 1, 2016: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/27574299/a-recombinant-chimeric-ad5-3-vector-expressing-a-multistage-plasmodium-antigen-induces-protective-immunity-in-mice-using-heterologous-prime-boost-immunization-regimens
#18
Monica Cabrera-Mora, Jairo Andres Fonseca, Balwan Singh, Chunxia Zhao, Natalia Makarova, Igor Dmitriev, David T Curiel, Jerry Blackwell, Alberto Moreno
An ideal malaria vaccine should target several stages of the parasite life cycle and induce antiparasite and antidisease immunity. We have reported a Plasmodium yoelii chimeric multistage recombinant protein (P. yoelii linear peptide chimera/recombinant modular chimera), engineered to express several autologous T cell epitopes and sequences derived from the circumsporozoite protein and the merozoite surface protein 1. This chimeric protein elicits protective immunity, mediated by CD4(+) T cells and neutralizing Abs...
October 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27538760/implantation-of-stromal-vascular-fraction-progenitors-at-bone-fracture-sites-from-a-rat-model-to-a-first-in-man-study
#19
Franziska Saxer, Arnaud Scherberich, Atanas Todorov, Patrick Studer, Sylvie Miot, Simone Schreiner, Sinan Güven, Laurent A H Tchang, Martin Haug, Michael Heberer, Dirk J Schaefer, Daniel Rikli, Ivan Martin, Marcel Jakob
Stromal Vascular Fraction (SVF) cells freshly isolated from adipose tissue include osteogenic- and vascular-progenitors, yet their relevance in bone fracture healing is currently unknown. Here, we investigated whether human SVF cells directly contribute to the repair of experimental fractures in nude rats, and explored the feasibility/safety of their clinical use for augmentation of upper arm fractures in elderly individuals. Human SVF cells were loaded onto ceramic granules within fibrin gel and implanted in critical nude rat femoral fractures after locking-plate osteosynthesis, with cell-free grafts as control...
August 19, 2016: Stem Cells
https://www.readbyqxmd.com/read/27499818/allogeneic-blood-product-usage-in-coronary-artery-bypass-grafting-cabg-with-minimalized-extracorporeal-circulation-system-mecc-versus-standard-on-pump-coronary-artery-bypass-grafting
#20
M Lisy, E Schmid, J Kozok, P Rosenberger, U A Stock, G Kalender
AIM: Intraoperative allogeneic blood product transfusion (ABPT) in cardiac surgery is associated with worse overall outcome, including mortality. The objective of this study was to evaluate the ABPTs in minimalized extracorporeal cardiopulmonary (MECC(TM)) compared with standard open system on-pump coronary revascularization. METHODS: Data of 156 patients undergoing myocardial revascularization between September 2008 and September 2010 were reviewed. 83 patients were operated by the MECC technique and 73 were treated by standard extracorporeal circulation (sECC)...
2016: Open Cardiovascular Medicine Journal
keyword
keyword
69572
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"