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Zhi Yang, Minqiang Wang, Qiang Zhao, Hengwei Qiu, Junjie Li, Xiangming Li, Jinyou Shao
The branched hierarchical heteronanowires have been widely studied for optoelectronics application due to their unique electronic and photonic performances. Here, we successfully synthesized Ag nanowire-ZnO branched nanorods heteronanowires based on improved hydrothermal method. Then we fabricated single heteronanowire across an Au electrode pairs with different gap width and parallel aligned heteronanowires on Au interdigitated electrode with dielectrophoresis method, indicating the flexibility and operability of dielectrophoresis assembly method...
June 16, 2017: ACS Applied Materials & Interfaces
James Froberg, Vidura Jayasooriya, Seungyong You, Dharmakeerthi Nawarathna, Yongki Choi
Nanoelectronic devices integrated with dielectrophoresis (DEP) have been promoted as promising platforms for trapping, separating, and concentrating target biomarkers and cancer cells from a complex medium. Here, we visualized DEP and DEP gradients in conventional nanoelectronic devices by using multi-pass atomic force microcopy techniques. Our measurements directly demonstrated a short range DEP only at sharp step edges of electrodes, frequency dependent DEP polarity, and separation distance dependent DEP strength...
May 15, 2017: Applied Physics Letters
Min Li, Robbyn K Anand
We demonstrate continuous high-throughput selective capture of circulating tumor cells by dielectrophoresis at arrays of wireless electrodes (bipolar electrodes, BPEs). The use of BPEs removes the requirement of ohmic contact to individual array elements, thus enabling otherwise unattainable device formats. Capacitive charging of the electrical double layer at opposing ends of each BPE allows an AC electric field to be transmitted across the entire device. Here, two such designs are described and evaluated...
June 21, 2017: Journal of the American Chemical Society
Sepeedah Soltanian-Zadeh, Kruthika Kikkeri, Ayesha N Shajahan-Haq, Jeannine Strobl, Robert Clarke, Masoud Agah
Inherent electrical properties of cells can be beneficial to characterize different cell lines and their response to experimental drugs. This paper presents a novel method to characterize the response of breast cancer cells to drug stimuli through use of off-chip passivated-electrode insulator-based dielectrophoresis (OπDEP) and the application of AC electric fields. This work is the first to demonstrate the ability of OπDEP to differentiate between two closely related breast cancer cell lines, LCC1 and LCC9 while assessing their drug sensitivity to an experimental anti-cancer agent, Obatoclax...
June 13, 2017: Electrophoresis
Diya Li, Ceming Wang, Gongchen Sun, Satyajyoti Senapati, Hsueh-Chia Chang
Detection and quantification of low-concentration proteins in heterogeneous media are generally plagued by two distinct obstacles: lack of sensitivity due to high dissociation equilibrium constant KD and non-specificity due to an abundance of non-targets with similar KD. Herein, we report a nanoscale protein-sensing platform with a non-equilibrium on-off switch that employs dielectrophoretic and hydrodynamic shear forces to overcome these thermodynamic limitations with irreversible kinetics. The detection sensitivity is achieved with complete association of the antibody-antigen-antibody (Ab-Ag-Ab) complex by precisely and rapidly assembling carbon nanotubes (CNT) across two parallel electrodes via sequential DC electrophoresis and AC dielectrophoresis (DEP), and with single-CNT electron tunneling conductance...
May 31, 2017: Biosensors & Bioelectronics
Edwar Iswardy, Tien-Chun Tsai, I-Fang Cheng, Tzu-Chuan Ho, Guey Chuen Perng, Hsien-Chang Chang
No abstract text is available yet for this article.
June 1, 2017: Biosensors & Bioelectronics
Linbo Liu, Ke Chen, Di Huang, Xin Wang, Nan Xiang, Zhonghua Ni
In this paper, we present a novel 'leadless' dielectrophoresis chip with dot matrix electrodes (LDME-DEP chip) fabricated by stacking three different functional layers. Our LDME-DEP chip excels mainly in two aspects: we for the first time applied the technique of separating the lead and the electrode pattern into two different layers to patterning nanowires which achieves continuous-area manipulation of nanowires without interference from the lead; the use of dot matrix electrodes makes the manipulation more flexible...
June 2, 2017: Nanotechnology
Song-I Han, Hyun Soo Kim, Arum Han
Concentrating cells or adjusting the concentration of cells are one of the most fundamental steps in cell biology experiments, and are typically achieved through centrifugation. However this step is challenging to implement in droplet microfluidics. Here we present an in-droplet cell concentrator that operates by first gradually focusing cells inside a droplet to one side of the droplet using negative dielectrophoresis (nDEP), followed by asymmetric droplet splitting using a Y-shaped junction, resulting in two daughter droplets, one of which containing all or most of the cells...
May 21, 2017: Biosensors & Bioelectronics
R Frisenda, E Giovanelli, P Mishra, P Gant, E Flores, C Sánchez, J R Ares, D Perez de Lara, I J Ferrer, E M Pérez, A Castellanos-Gomez
Liquid-phase exfoliation is a technique capable of producing large quantities of two-dimensional materials in suspension. Despite many efforts in the optimization of the exfoliation process itself, not much has been done towards the integration of liquid-phase-exfoliated materials in working solid-state devices. In this article, we use dielectrophoresis to direct the assembly of liquid-phase-exfoliated TiS3 nanoribbons between two gold electrodes to produce photodetectors working in the visible region. Through electrical and optical measurements we characterize the responsivity of the device and we find values as large as 3...
June 1, 2017: Chemical Communications: Chem Comm
Yuhao Qiang, Jia Liu, E Du
Erythrocytes must undergo severe deformation to pass through narrow capillaries and submicronic splenic slits for several hundred thousand times in their normal lifespan. Studies of erythrocyte biomechanics have been mainly focused on cell deformability and rheology measured from a single application of stress and mostly under a static or quasi-static state using classical biomechanical techniques, such as optical tweezers and micropipette aspiration. Dynamic behavior of erythrocytes in response to cyclic stresses that contributes to the membrane failure in blood circulation is not fully understood...
May 17, 2017: Acta Biomaterialia
Shuangxi Xie, Xiaodong Wang, Niandong Jiao, Steve Tung, Lianqing Liu
Trapping and transporting microorganisms with intrinsic motility are important tasks for biological, physical, and biomedical applications. However, fast swimming speed makes the manipulation of these organisms an inherently challenging task. In this study, we demonstrated that an optoelectrical technique, namely, optically induced dielectrophoresis (ODEP), could effectively trap and manipulate Chlamydomonas reinhardtii (C. reinhardtii) cells swimming at velocities faster than 100 μm s(-1). Furthermore, live C...
June 13, 2017: Lab on a Chip
Yu-Liang Chen, Hong-Ren Jiang
We demonstrate a functional rotating electrothermal technique for rapidly concentrating and sorting a large number of particles on a microchip by the combination of particle dielectrophoresis (DEP) and inward rotating electrothermal (RET) flows. Different kinds of particles can be attracted (positive DEP) to or repelled (negative DEP) from electrode edges, and then the n-DEP responsive particles are further concentrated in the heated region by RET flows. The RET flows arise from the spatial inhomogeneous electric properties of fluid caused by direct infrared laser (1470 nm) heating of solution in a rotating electric field...
May 2017: Biomicrofluidics
Zbigniew Rozynek, Ming Han, Filip Dutka, Piotr Garstecki, Arkadiusz Józefczak, Erik Luijten
One-dimensional conductive particle assembly holds promise for a variety of practical applications, in particular for a new generation of electronic devices. However, synthesis of such chains with programmable shapes outside a liquid environment has proven difficult. Here we report a route to simply 'pull' flexible granular and colloidal chains out of a dispersion by combining field-directed assembly and capillary effects. These chains are automatically stabilized by liquid bridges formed between adjacent particles, without the need for continuous energy input or special particle functionalization...
May 12, 2017: Nature Communications
Oscar I Bernal, Bhuvnesh Bharti, Michael C Flickinger, Orlin D Velev
We report how dielectrophoresis (DEP) can be used as a tool for the fabrication of biocomposite coatings of photoreactive cyanobacteria (Synechococcus PCC7002) on flexible polyester sheets (PEs). The PE substrates were precoated by a layer-by-layer assembled film of charged polyelectrolytes. In excellent agreement between experimental data and numerical simulations, the directed assembly process driven by external electric field results in the formation of 1D chains and 2D sheets by the cells. The preassembled cyanobacteria chains and arrays became deposited on the substrate and remained in place after the electric field was turned off due to the electrostatic attraction between the negatively charged cell surfaces and the positively charged polyelectrolyte-coated PE...
May 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Peilin Zhou, Haibo Yu, Wenguang Yang, Yangdong Wen, Zhidong Wang, Wen Jung Li, Lianqing Liu
In this article, we present a novel method of spatial manipulation and assembly of nanoparticles via atomic force microscopy tip-induced dielectrophoresis (AFM-DEP). This method combines the high-accuracy positioning of AFM with the parallel manipulation of DEP. A spatially nonuniform electric field is induced by applying an alternating current (AC) voltage between the conductive AFM probe and an indium tin oxide glass substrate. The AFM probe acted as a movable DEP tweezer for nanomanipulation and assembly of nanoparticles...
May 8, 2017: ACS Applied Materials & Interfaces
Hsiang-Chen Wang, Ngoc-Viet Nguyen, Rui-Yi Lin, Chun-Ping Jen
Analysis of cancerous cells allows us to provide useful information for the early diagnosis of cancer and to monitor treatment progress. An approach based on electrical principles has recently become an attractive technique. This study presents a microdevice that utilizes a dielectrophoretic impedance measurement method for the identification of cancerous cells. The proposed biochip consists of circle-on-line microelectrodes that are patterned using a standard microfabrication processes. A sample of various cell concentrations was introduced in an open-top microchamber...
May 6, 2017: Sensors
Rodrigo Martinez-Duarte
The focus of this review is to assess the current status of three-dimensional (3D) carbon-electrode dielectrophoresis (carbonDEP) and identify the challenges currently preventing it from its use in high-throughput applications such as sample preparation for diagnostics. The use of 3D electrodes over more traditional planar ones is emphasised here as a way to increase the throughput of DEP devices. Glass-like carbon electrodes are derived through the carbonisation of photoresist structures made using photolithography...
March 2017: IET Nanobiotechnology
Ali Rohani, John H Moore, Jennifer A Kashatus, Hiromi Sesaki, David F Kashatus, Nathan S Swami
Mitochondrial dynamics play an important role within several pathological conditions, including cancer and neurological diseases. For the purpose of identifying therapies that target aberrant regulation of the mitochondrial dynamics machinery and characterizing the regulating signaling pathways, there is a need for label-free means to detect the dynamic alterations in mitochondrial morphology. We present the use of dielectrophoresis for label-free quantification of intracellular mitochondrial modifications that alter cytoplasmic conductivity, and these changes are benchmarked against label-based image analysis of the mitochondrial network...
June 6, 2017: Analytical Chemistry
Blanca H Lapizco-Encinas
No abstract text is available yet for this article.
June 2017: Electrophoresis
Jordon Gilmore, Monsur Islam, Josie Duncan, Rucha Natu, Rodrigo Martinez-Duarte
No abstract text is available yet for this article.
May 3, 2017: Electrophoresis
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