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https://www.readbyqxmd.com/read/27900736/preparation-of-horizontal-single-walled-carbon-nanotubes-arrays
#1
REVIEW
Pan Li, Jin Zhang
The synthesis of SWNTs has achieved great success with the development of synthetic methodologies. From the viewpoint of exploiting the exceptional electrical properties of single-walled carbon nanotubes (SWNTs) in advanced applications, one of the most difficult challenges is how to assemble the SWNTs with high degrees of alignment and purity in electronic conducting (mainly semiconducting) behavior into functional nanodevices. Numerous approaches have been developed to reach this goal, which could be divided into two categories...
December 2016: Topics in Current Chemistry (Journal)
https://www.readbyqxmd.com/read/27883074/adipose-stem-cells-display-higher-regenerative-capacities-and-more-adaptable-electro-kinetic-properties-compared-to-bone-marrow-derived-mesenchymal-stromal-cells
#2
Ahmed El-Badawy, Marwa Amer, Reda Abdelbaset, Sameh N Sherif, Marwan Abo-Elela, Yehya H Ghallab, Hamdy Abdelhamid, Yehea Ismail, Nagwa El-Badri
Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27829628/alternation-of-gene-expression-levels-in-mesenchymal-stem-cells-by-applying-positive-dielectrophoresis
#3
Junya Yoshioka, Toru Yoshitomi, Tomoyuki Yasukawa, Keitaro Yoshimoto
In this study, we investigated the effect of positive dielectrophoresis (DEP) on gene expression in mesenchymal stem cells. When applying an alternating current voltage, human bone marrow derived mesenchymal stem cells (UE7T-13) exhibited a positive DEP, and were compressed onto the electrode surface. The constructed device can easily control the DEP force to the cells by changing the frequency. Interestingly, gene expressions of the cell differentiation marker in UE7T-13 cells and the mechanical stimulation-susceptible one were changed by applying a positive DEP...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27822330/adhesion-patterning-by-a-novel-air-lock-technique-enables-localization-and-in-situ-real-time-imaging-of-reprogramming-events-in-one-to-one-electrofused-hybrids
#4
S Sakamoto, K O Okeyo, S Yamazaki, O Kurosawa, H Oana, H Kotera, M Washizu
Although fusion of somatic cells with embryonic stem (ES) cells has been shown to induce reprogramming, single-cell level details of the transitory phenotypic changes that occur during fusion-based reprogramming are still lacking. Our group previously reported on the technique of one-to-one electrofusion via micro-slits in a microfluidic platform. In this study, we focused on developing a novel air-lock patterning technique for creating localized adhesion zones around the micro-slits for cell localization and real-time imaging of post fusion events with a single-cell resolution...
September 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27819046/constructing-3d-heterogeneous-hydrogels-from-electrically-manipulated-prepolymer-droplets-and-crosslinked-microgels
#5
Min-Yu Chiang, Yao-Wen Hsu, Hsin-Yi Hsieh, San-Yuan Chen, Shih-Kang Fan
Formation of multifunctional, heterogeneous, and encoded hydrogel building blocks, or microgels, by crosslinking and assembly of microgels are two essential steps in establishing hierarchical, complicated, and three-dimensional (3D) hydrogel architectures that recapitulate natural and biological structures or originate new materials by design. However, for the variety of the hydrogel materials crosslinked differently and for the varied scales of microgels and architectures, the formation and assembly processes are usually performed separately, which increases the manufacturing complexity of designed hydrogel materials...
October 2016: Science Advances
https://www.readbyqxmd.com/read/27815893/overview-of-cell-synchronization
#6
Gaspar Banfalvi
The widespread interest in cell synchronization is maintained by the studies of control mechanism involved in cell cycle regulation. During the synchronization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphorylation, development of new drugs, etc. Although several synchronization methods have been described, it is of general interest that scientists get a compilation and an updated view of these synchronization techniques...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27775917/a-carbon-nanotube-based-x-ray-detector
#7
Richard A Boucher, Jürgen Bauch, Dietmar Wünsche, Gerhard Lackner, Anindya Majumder
X-ray detectors based on metal-oxide semiconductor field effect transistors couple instantaneous measurement with high accuracy. However, they only have a limited measurement lifetime because they undergo permanent degradation due to x-ray beam exposure. A field effect transistor based on carbon nanotubes (CNTs), however, overcomes this drawback of permanent degradation, because it can be reset into its starting state after being exposed to the x-ray beam. In this work the CNTs were deposited using a dielectrophoresis method on SiO2 coated p-type (boron-doped) Si substrates...
October 24, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27775531/dielectrophoretic-relay-assisted-molecular-communication-for-in-sequence-molecule-delivery
#8
Puneet Manocha, Gitanjali Chandwani, Soumen Das
With current research focus to interconnect the molecular communication environment with external environment, it is imperative to design external devices working on molecular communication schemes to be interfaced with in-vivo molecular network. Recently, efforts have been made to integrate molecular communication with Lab-on-chip (LOC); one of the techniques used in LOC for manipulation and transportation of molecules is Dielectrophoresis (DEP). We propose the use of DEP in molecular communication to maintain in-sequence delivery of molecules...
October 19, 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/27775528/characterization-of-drug-effect-on-leukemia-cells-through-single-cell-assay-with-optical-tweezers-and-dielectrophoresis
#9
Jundi Hou, Tao Luo, Ka Lam Ng, Raymond Liang, Anskar Y H Leung, Dong Sun
One of the greatest challenges in acute myeloid leukemia (AML) treatment is preventing relapse. Leukemia cells can hide in bone marrow niche or vascular niche. Hence, many chemical drugs cannot kill these cells. To characterize migration and adhesion properties of leukemia cells in specific niches, CXCR4/SDF-1α signal pathway has been widely used for investigation. AMD3100 is treated as one of the most common chemical drugs that can inhibit this signal. In the current study, we particularly investigate the effect of AMD3100 on the adhesion property of leukemia cells on stromal cells by using engineering tools, namely, optical tweezers (OT) and dielectrophoresis (DEP), to probe single cell property...
October 19, 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/27775139/separation-of-nanoparticles-by-a-nano-orifice-based-dc-dielectrophoresis-method-in-a-pressure-driven-flow
#10
Kai Zhao, Ran Peng, Dongqing Li
A novel DC-dielectrophoresis (DEP) method employing a pressure-driven flow for the continuous separation of micro/nano-particles is presented in this paper. To generate the DEP force, a small voltage difference is applied to produce a non-uniformity of the electric field across a microchannel via a larger orifice of several hundred microns on one side of the channel wall and a smaller orifice of several hundred nanometers on the opposite channel wall. The particles experience a DEP force when they move with the flow through the vicinity of the small orifice, where the strongest electrical field gradient exists...
December 7, 2016: Nanoscale
https://www.readbyqxmd.com/read/27774532/accurate-quantification-of-apoptosis-progression-and-toxicity-using-a-dielectrophoretic-approach
#11
Erin A Henslee, Ruth M Torcal Serrano, Fatima H Labeed, Rita I Jabr, Christopher H Fry, Michael P Hughes, Kai F Hoettges
A loss of ability of cells to undergo apoptosis (programmed cell death, whereby the cell ceases to function and destroys itself) is commonly associated with cancer, and many anti-cancer interventions aim to restart the process. Consequently, the accurate quantification of apoptosis is essential in understanding the function and performance of new anti-cancer drugs. Dielectrophoresis has previously been demonstrated to detect apoptosis more rapidly than other methods, and is low-cost, label-free and rapid, but has previously been unable to accurately quantify cells through the apoptotic process because cells in late apoptosis disintegrate, making cell tracking impossible...
October 24, 2016: Analyst
https://www.readbyqxmd.com/read/27747893/electric-field-mediated-spraying-of-miniaturized-droplets-inside-microchannel
#12
Seim Timung, Joydip Chaudhuri, Manash Pratim Borthakur, Tapas Kumar Mandal, Gautam Biswas, Dipankar Bandyopadhyay
We report a facile and non-invasive way to disintegrate a microdroplet into a string of further miniaturized ones under the influence of an external electrohydrodynamic field inside a microchannel. The deformation and breakup of the droplet was engendered by the Maxwell's stress originating from the accumulation of induced and free charges at the oil-water interface. While at smaller field intensities, e.g. less than 1 MV/m, the droplet deformed into a plug, at relatively higher field intensities, e.g. ∼1...
October 17, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27747354/effect-of-electric-field-non-uniformity-on-droplets-coalescence
#13
Shirui Luo, Jarrod Schiffbauer, Tengfei Luo
Electric field assisted coalescence is one of the most efficient methods for water-in-oil emulsion separation. In this paper, we experimentally study water droplet evolution in an oil phase under different electric field configurations. We determine that non-uniform fields can enhance the performance of electrocoalescence compared to uniform fields. The analysis indicates that the enhanced coalescence is due to the combined effects of dipole-dipole interaction between droplets and dielectrophoresis between individual droplets and the applied non-uniform field...
October 17, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27722555/monitoring-sepsis-using-electrical-cell-profiling
#14
Javier L Prieto, Hao-Wei Su, Han Wei Hou, Miguel Pinilla Vera, Bruce D Levy, Rebecca M Baron, Jongyoon Han, Joel Voldman
Sepsis is a potentially lethal condition that may be ameliorated through early monitoring of circulating activated leukocytes for faster stratification of severity of illness and improved administration of targeted treatment. Characterization of the intrinsic electrical properties of leukocytes is label-free and can provide a quick way to quantify the number of activated cells as sepsis progresses. Iso-dielectric separation (IDS) uses dielectrophoresis (DEP) to characterize the electrical signatures of cells...
October 7, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27704042/not-spreading-in-reverse-the-dewetting-of-a-liquid-film-into-a-single-drop
#15
Andrew M J Edwards, Rodrigo Ledesma-Aguilar, Michael I Newton, Carl V Brown, Glen McHale
Wetting and dewetting are both fundamental modes of motion of liquids on solid surfaces. They are critically important for processes in biology, chemistry, and engineering, such as drying, coating, and lubrication. However, recent progress in wetting, which has led to new fields such as superhydrophobicity and liquid marbles, has not been matched by dewetting. A significant problem has been the inability to study the model system of a uniform film dewetting from a nonwetting surface to a single macroscopic droplet-a barrier that does not exist for the reverse wetting process of a droplet spreading into a film...
September 2016: Science Advances
https://www.readbyqxmd.com/read/27703590/sheathless-electrokinetic-particle-separation-in-a-bifurcating-microchannel
#16
Di Li, Xinyu Lu, Yongxin Song, Junsheng Wang, Dongqing Li, Xiangchun Xuan
Particle separation has found practical applications in many areas from industry to academia. Current electrokinetic particle separation techniques primarily rely on dielectrophoresis, where the electric field gradients are generated by either active microelectrodes or inert micro-insulators. We develop herein a new type of electrokinetic method to continuously separate particles in a bifurcating microchannel. This sheath-free separation makes use of the inherent wall-induced electrical lift to focus particles towards the centerline of the main-branch and then deflect them to size-dependent flow paths in each side-branch...
September 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27700108/towards-single-electron-nanoelectronics-using-self-assembled-dna-structure
#17
Kosti Tuomas Olavi Tapio, Jenni Leppiniemi, Boxuan Shen, Vesa P Hytönen, Wolfgang Fritzsche, J Jussi Toppari
DNA based structures offer an adaptable and robust way to develop customized nanostructures for various purposes in bionanotechnology. One main aim in this field is to develop a DNA nano-breadboard for a controllable attachment of nanoparticles or biomolecules to form specific nanoelectronic devices. Here we conjugate three gold nanoparticles on a defined size TX-tile assembly into a linear pattern to form nanometer scale isolated islands that could be utilized in a room temperature single electron transistor...
October 4, 2016: Nano Letters
https://www.readbyqxmd.com/read/27690464/near-axisymmetric-partial-wetting-using-interface-localized-liquid-dielectrophoresis
#18
Zuzana Brabcova, Glen McHale, Gary George Wells, Carl V Brown, Michael Ian Newton, Andrew M J Edwards
The wetting of solid surfaces can be modified by altering the surface free energy balance between the solid, liquid, and vapour phases. Liquid dielectrophoresis (L-DEP) can produce wetting on normally non-wetting surfaces, without modification of the surface topography or chemistry. L-DEP is a bulk force acting on the dipoles of a dielectric liquid and is not normally considered to be a localized effect acting at the interface between the liquid and a solid or other fluid. However, if this force is induced by a non-uniform electric field across a solid-liquid interface, it can be used to enhance and control the wetting of a dielectric liquid...
October 3, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27676373/emerging-microfluidic-devices-for-cancer-cells-biomarkers-manipulation-and-detection
#19
Victor Hugo Perez-Gonzalez, Roberto Carlos Gallo-Villanueva, Sergio Camacho-Leon, Jose Isabel Gomez-Quiñones, Jose Manuel Rodriguez-Delgado, Sergio Omar Martinez-Chapa
Circulating tumour cells (CTCs) are active participants in the metastasis process and account for ∼90% of all cancer deaths. As CTCs are admixed with a very large amount of erythrocytes, leukocytes, and platelets in blood, CTCs are very rare, making their isolation, capture, and detection a major technological challenge. Microfluidic technologies have opened-up new opportunities for the screening of blood samples and the detection of CTCs or other important cancer biomarker-proteins. In this study, the authors have reviewed the most recent developments in microfluidic devices for cells/biomarkers manipulation and detection, focusing their attention on immunomagnetic-affinity-based devices, dielectrophoresis-based devices, surface-plasmon-resonance microfluidic sensors, and quantum-dots-based sensors...
October 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27649201/combined-dielectrophoresis-and-impedance-systems-for-bacteria-analysis-in-microfluidic-on-chip-platforms
#20
Cristina Páez-Avilés, Esteve Juanola-Feliu, Jaime Punter-Villagrasa, Beatriz Del Moral Zamora, Antoni Homs-Corbera, Jordi Colomer-Farrarons, Pere Lluís Miribel-Català, Josep Samitier
Bacteria concentration and detection is time-consuming in regular microbiology procedures aimed to facilitate the detection and analysis of these cells at very low concentrations. Traditional methods are effective but often require several days to complete. This scenario results in low bioanalytical and diagnostic methodologies with associated increased costs and complexity. In recent years, the exploitation of the intrinsic electrical properties of cells has emerged as an appealing alternative approach for concentrating and detecting bacteria...
2016: Sensors
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