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dielectrophoresis

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https://www.readbyqxmd.com/read/28328089/capacitive-dna-sensor-for-rapid-and-sensitive-detection-of-whole-genome-human-herpes-virus-1-dsdna-in-serum
#1
Cheng Cheng, Rania Oueslati, Jayne Wu, Jiangang Chen, Shigetoshi Eda
This work presents a rapid, highly sensitive, low-cost and specific capacitive DNA sensor for detection of whole genome human herpes virus-1 DNA. This sensor is capable of direct DNA detection with a response time of 30 seconds, and it can be used to test standard buffer or serum samples. The sensing approach for DNA detection is based on AC electrokinetics. By applying an inhomogeneous AC electric field on sensor electrodes, positive dielectrophoresis is induced to accelerate DNA hybridization. The same applied AC signal also directly measures the hybridization of target with the probe on the sensor surface...
March 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28328070/modeling-and-simulation-of-dielectrophoretic-collective-dynamics-in-a-suspension-of-polarizable-particles-under-the-action-of-a-gradient-ac-electric-field
#2
Shigeru Tada, Yan Shen, Zhiyong Qiu
When a suspension of polarizable particles is subjected to a gradient AC electric field, the particles exhibit collective motion due to an interaction between the dipole induced in the particles and the spatial gradient of the electric field; this is known as dielectrophoresis. In the present study, the collective dynamics of suspended particles in a parallel-plate electric chamber was investigated by simulating numerically the trajectories of individual particles under the action of combined dielectrophoretic and dipole-dipole interparticle forces...
March 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28314101/a-dielectrophoresis-system-for-testing-antimicrobial-susceptibility-of-gram-negative-bacteria-to-beta-lactam-antibiotics
#3
I-Hsiu Su, Wen-Chien Ko, Chung-Hsin Shih, Fang-Hao Yeh, Yung-Nien Sun, Jung-Chih Chen, Po-Lin Chen, Hsien-Chang Chang
Gram-negative bacteria (GNBs) are common pathogens causing severe sepsis. Rapid evaluation of drug susceptibility would guide effective antibiotic treatment, and promote life-saving. A total of 78 clinical isolates of 13 gram-negative species collected between April 2013 and November 2013 from two medical centers in Tainan were tested. Bacterial morphology changes in different concentrations of antibiotics were observed under the electric field of a quadruple electrode array using light microscopy. The minimal inhibitory concentrations (MICs) of four antimicrobial agents, namely cefazolin, ceftazidime, cefepime, and doripenem, were determined by the dielectrophoretic antimicrobial susceptibility testing (dAST) and by the conventional broth dilution testing (BDT)...
March 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28306161/dna-dielectrophoresis-theory-and-applications-a-review
#4
REVIEW
Martina Viefhues, Ralf Eichhorn
Dielectrophoresis is the migration of an electrically polarizable particle in an inhomogeneous electric field. This migration can be exploited for several applications with (bio)molecules or cells. Dielectrophoresis is a non-invasive technique; therefore, it is very convenient for (selective) manipulation of (bio)molecules or cells. In this review, we will focus on DNA dielectrophoresis as this technique offers several advantages in trapping and immobilization, separation and purification, and analysis of DNA molecules...
March 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28301054/dipole-and-multipole-models-of-dielectrophoresis-for-a-non-negligible-particle-size-simulations-and-experiments
#5
Tomáš Michálek, Jiří Zemánek
Mathematical models of dielectrophoresis play an important role in the design of experiments, analysis of results, and even operation of some devices. In this paper, we test the accuracy of existing models in both simulations and laboratory experiments. We test the accuracy of the most common model that involves a point-dipole approximation of the induced field, when the small-particle assumption is broken. In simulations, comparisons against a model based on the Maxwell stress tensor show that even the point-dipole approximation provides good results for a large particle close to the electrodes...
March 16, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28295737/dielectrophoresis-assisted-integration-of-1024-carbon-nanotube-sensors-into-a-cmos-microsystem
#6
Florent Seichepine, Jörg Rothe, Alexandra Dudina, Andreas Hierlemann, Urs Frey
Carbon-nanotube (CNT)-based sensors offer the potential to detect single-molecule events and picomolar analyte concentrations. An important step toward applications of such nanosensors is their integration in large arrays. The availability of large arrays would enable multiplexed and parallel sensing, and the simultaneously obtained sensor signals would facilitate statistical analysis. A reliable method to fabricate an array of 1024 CNT-based sensors on a fully processed complementary-metal-oxide-semiconductor microsystem is presented...
March 15, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28289483/dielectrophoresis-study-of-temporal-change-in-internal-conductivity-of-single-cho-cells-after-electroporation-by-pulsed-electric-fields
#7
E Salimi, K Braasch, M Butler, D J Thomson, G E Bridges
Applying sufficiently strong pulsed electric fields to a cell can permeabilize the membrane and subsequently affect its dielectric properties. In this study, we employ a microfluidic dielectrophoresis cytometry technique to simultaneously electroporate and measure the time-dependent dielectric response of single Chinese hamster ovary cells. Using experimental measurements along with numerical simulations, we present quantitative results for the changes in the cytoplasm conductivity of single cells within seconds after exposure to 100 μs duration pulsed electric fields with various intensities...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28276545/isolation-and-concentration-of-bacteria-from-blood-using-microfluidic-membraneless-dialysis-and-dielectrophoresis
#8
L D'Amico, N J Ajami, J A Adachi, P R C Gascoyne, J F Petrosino
A microfluidic system that combines membraneless microfluidic dialysis and dielectrophoresis to achieve label-free isolation and concentration of bacteria from whole blood is presented. Target bacteria and undesired blood cells are discriminated on the basis of their differential susceptibility to permeabilizing agents that alter the dielectrophoretic behavior of blood cells but not bacteria. The combined membraneless microdialysis and dielectrophoresis system isolated 79 ± 3% of Escherichia coli and 78 ± 2% of Staphylococcus aureus spiked into whole blood at a processing rate of 0...
March 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28268374/sheathless-and-high-throughput-sorting-of-paramagnetic-microparticles-in-a-magneto-hydrodynamic-microfluidic-device
#9
Vikash Kumar, Pouya Rezai
Sorting of microorganisms and particles from a mixture is critical for numerous biotechnological and medical applications. Several sorting methods such as pinched flow fractionation (PFF), optical sorting, dielectrophoresis, acoustic separation, magnetophoresis and deterministic lateral displacement (DLD) have been reported in literature. But most of these methods lack ideal characteristics of a sorter such as ability to process at high throughput, simple design, non-complicated fabrication method, sheathless operation and high purity in separation...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28256738/dielectrophoresis-assisted-loading-and-unloading-of-micro-wells-for-impedance-spectroscopy
#10
Amin Mansoorifar, Anil Koklu, Ahmet Can Sabuncu, Ali Beskok
Dielectric spectroscopy (DS) is a non-invasive, label-free, fast, and promising technique for measuring dielectric properties of biological cells in real time. We demonstrate a microchip that consists of electro-activated micro-well arrays for positive dielectrophoresis (pDEP) assisted cell capture, DS measurements, and negative dielectrophoresis (nDEP) driven cell unloading; thus, providing a high throughput cell analysis platform. To the best of our knowledge, this is the first microfluidic chip that combines electro-activated micro-wells and DS to analyze biological cells...
March 3, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28245552/dielectrophoresis-for-biomedical-sciences-applications-a-review
#11
REVIEW
Nurhaslina Abd Rahman, Fatimah Ibrahim, Bashar Yafouz
Dielectrophoresis (DEP) is a label-free, accurate, fast, low-cost diagnostic technique that uses the principles of polarization and the motion of bioparticles in applied electric fields. This technique has been proven to be beneficial in various fields, including environmental research, polymer research, biosensors, microfluidics, medicine and diagnostics. Biomedical science research is one of the major research areas that could potentially benefit from DEP technology for diverse applications. Nevertheless, many medical science research investigations have yet to benefit from the possibilities offered by DEP...
February 24, 2017: Sensors
https://www.readbyqxmd.com/read/28226538/sheathless-and-high-throughput-sorting-of-paramagnetic-microparticles-in-a-magneto-hydrodynamic-microfluidic-device
#12
Vikash Kumar, Pouya Rezai, Vikash Kumar, Pouya Rezai, Vikash Kumar, Pouya Rezai
Sorting of microorganisms and particles from a mixture is critical for numerous biotechnological and medical applications. Several sorting methods such as pinched flow fractionation (PFF), optical sorting, dielectrophoresis, acoustic separation, magnetophoresis and deterministic lateral displacement (DLD) have been reported in literature. But most of these methods lack ideal characteristics of a sorter such as ability to process at high throughput, simple design, non-complicated fabrication method, sheathless operation and high purity in separation...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28217772/dielectrowetting-manipulation-for-digital-microfluidics-creating-transporting-splitting-and-merging-of-droplets
#13
Hongyao Geng, Jian Feng, Lisa Marie Stabryla, Sung Kwon Cho
Generating, splitting, transporting, and merging droplets are fundamental and critical unit operations for digital (droplet-based) microfluidics. State-of-the-art digital microfluidics performs such operations commonly using electrowetting-on-dielectric (EWOD) in the typical configuration of two parallel channel plates. This paper presents such operations using dielectrowetting (derived from liquid dielectrophoresis), not EWOD, with an array of interdigitated electrodes. The major and unique feature is that the present droplet manipulations are effective for conductive (water with/without surfactant) and non-conductive (propylene carbonate) fluids...
March 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28215153/engineered-muscle-tissues-for-disease-modeling-and-drug-screening-applications
#14
Mohammad Hossein Mohammadi, Raquel Obregón, Samad Ahadian, Javier Ramón-Azcón, Milica Radisic
Animal models have been the main resources for drug discovery and prediction of drugs' pharmacokinetic responses in the body. However, noticeable drawbacks associated with animal models include high cost, low reproducibility, low physiological similarity to humans, and ethical problems. Engineered tissue models have recently emerged as an alternative or substitute for animal models in drug discovery and testing and disease modeling. In this review, we focus on skeletal muscle and cardiac muscle tissues by first describing their characterization and physiology...
February 15, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28211116/ac-dielectrophoretic-manipulation-and-electroporation-of-vaccinia-virus-using-carbon-nanoelectrode-arrays
#15
Foram Ranjeet Madiyar, Sherry Basset, Omer Farooq, Stefan Rothenburg, Christopher Culbertson, Jun Li
This paper reports the capture and detection of vaccinia virus particles based on AC dielectrophoresis (DEP) and electrochemical impedance measurements employing an embedded vertically aligned carbon nanofiber (VACNF) nanoelectrode array (NEA) versus a macroscopic indium-tin-oxide (ITO) transparent electrode in a "points-and-lid" configuration. The nano-DEP device was fabricated by bonding two SU-8 covered electrodes patterned using photolithography. The bottom electrode contains a 200 × 200 μm(2) active region on a randomly distributed NEA and the top electrode contains a microfluidic channel in SU-8 spin-coated on ITO to guide the flow of the virus solution...
February 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28208767/alternative-cdep-design-to-facilitate-cell-isolation-for-identification-by-raman-spectroscopy
#16
Cynthia Hanson, Elizabeth Vargis
Dielectrophoresis (DEP) uses non-uniform electric fields to cause motion in particles due to the particles' intrinsic properties. As such, DEP is a well-suited label-free means for cell sorting. Of the various methods of implementing DEP, contactless dielectrophoresis (cDEP) is advantageous as it avoids common problems associated with DEP, such as electrode fouling and electrolysis. Unfortunately, cDEP devices can be difficult to fabricate, replicate, and reuse. In addition, the operating parameters are limited by the dielectric breakdown of polydimethylsiloxane (PDMS)...
February 9, 2017: Sensors
https://www.readbyqxmd.com/read/28187910/analysis-of-gap-junctional-intercellular-communications-using-a-dielectrophoresis-based-microchip
#17
Marta Tellez-Gabriel, Céline Charrier, Bénédicte Brounais-Le Royer, Mathilde Mullard, Hannah K Brown, Franck Verrecchia, Dominique Heymann
Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations...
February 6, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28164365/modeling-of-dielectrophoretic-particle-motion-point-particle-versus-finite-sized-particle
#18
Barbaros Çetin, S Doğan Öner, Besim Baranoğlu
Dielectrophoresis (DEP) is a very popular technique for microfluidic bio-particle manipulation. For the design of a DEP-based microfluidic device, simulation of the particle trajectory within the microchannel network is crucial. There are basically two approaches: (i) point-particle approach and (ii) finite-sized particle approach. In this study, many aspects of both approaches are discussed for the simulation of direct current DEP, alternating current DEP, and traveling-wave DEP applications. Point-particle approach is implemented using Lagrangian tracking method, and finite-sized particle is implemented using boundary element method...
February 6, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28150746/rapid-cell-separation-with-minimal-manipulation-for-autologous-cell-therapies
#19
Alban J Smith, Richard D O'Rorke, Akshay Kale, Roberts Rimsa, Matthew J Tomlinson, Jennifer Kirkham, A Giles Davies, Christoph Wälti, Christopher D Wood
The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28130914/enhancement-of-dielectrophoresis-using-fractal-gold-nanostructured-electrodes
#20
Anil Koklu, Ahmet C Sabuncu, Ali Beskok
Dielectrophoretic motions of Saccharomyces Cerevisiae (yeast) cells and colloidal gold are investigated using electrochemically modified electrodes exhibiting fractal topology. Electrodeposition of gold on electrodes generated repeated patterns with a fern-leaf type self-similarity. A particle tracking algorithm is used to extract dielectrophoretic particle velocities using fractal and planar electrodes in two different medium conductivities. The results show increased dielectrophoretic force when using fractal electrodes...
January 28, 2017: Electrophoresis
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