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dielectrophoresis

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https://www.readbyqxmd.com/read/28534561/dielectrophoretic-assembly-of-liquid-phase-exfoliated-tis3-nanoribbons-for-photodetecting-applications
#1
R Frisenda, E Giovanelli, P Mishra, P Gant, E Flores, C Sánchez, J R Ares, D Perez de Lara, I J Ferrer, E M Pérez, A Castellanos-Gomez
Liquid-phase exfoliation is a technique capable of producing large quantities of two-dimensional materials in suspension. Despite many efforts in the optimization of the exfoliation process itself, not much has been done towards the integration of liquid-phase-exfoliated materials in working solid-state devices. In this article, we use dielectrophoresis to direct the assembly of liquid-phase-exfoliated TiS3 nanoribbons between two gold electrodes to produce photodetectors working in the visible region. Through electrical and optical measurements we characterize the responsivity of the device and we find values as large as 3...
May 23, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28526627/dynamic-fatigue-measurement-of-human-erythrocytes-using-dielectrophoresis
#2
Yuhao Qiang, Jia Liu, E Du
Erythrocytes must undergo severe deformation to pass through narrow capillaries and submicronic splenic slits for several hundred thousand times in their normal lifespan. Studies of erythrocyte biomechanics have been mainly focused on cell deformability and rheology measured from a single application of stress and mostly under a static or quasi-static state using classical biomechanical techniques, such as optical tweezers and micropipette aspiration. Dynamic behavior of erythrocytes in response to cyclic stresses that contributes to the membrane failure in blood circulation is not fully understood...
May 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28513721/programmable-micrometer-sized-motor-array-based-on-live-cells
#3
Shuangxi Xie, Xiaodong Wang, Niandong Jiao, Steve Tung, Lianqing Liu
Trapping and transporting microorganisms with intrinsic motility are important tasks for biological, physical, and biomedical applications. However, fast swimming speed makes the manipulation of these organisms an inherently challenging task. In this study, we demonstrated that an optoelectrical technique, namely, optically induced dielectrophoresis (ODEP), could effectively trap and manipulate Chlamydomonas reinhardtii (C. reinhardtii) cells swimming at velocities faster than 100 μm s(-1). Furthermore, live C...
May 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28503246/particle-concentrating-and-sorting-under-a-rotating-electric-field-by-direct-optical-liquid-heating-in-a-microfluidics-chip
#4
Yu-Liang Chen, Hong-Ren Jiang
We demonstrate a functional rotating electrothermal technique for rapidly concentrating and sorting a large number of particles on a microchip by the combination of particle dielectrophoresis (DEP) and inward rotating electrothermal (RET) flows. Different kinds of particles can be attracted (positive DEP) to or repelled (negative DEP) from electrode edges, and then the n-DEP responsive particles are further concentrated in the heated region by RET flows. The RET flows arise from the spatial inhomogeneous electric properties of fluid caused by direct infrared laser (1470 nm) heating of solution in a rotating electric field...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28497791/formation-of-printable-granular-and-colloidal-chains-through-capillary-effects-and-dielectrophoresis
#5
Zbigniew Rozynek, Ming Han, Filip Dutka, Piotr Garstecki, Arkadiusz Józefczak, Erik Luijten
One-dimensional conductive particle assembly holds promise for a variety of practical applications, in particular for a new generation of electronic devices. However, synthesis of such chains with programmable shapes outside a liquid environment has proven difficult. Here we report a route to simply 'pull' flexible granular and colloidal chains out of a dispersion by combining field-directed assembly and capillary effects. These chains are automatically stabilized by liquid bridges formed between adjacent particles, without the need for continuous energy input or special particle functionalization...
May 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28481540/fabrication-of-photoreactive-biocomposite-coatings-via-electric-field-assisted-assembly-of-cyanobacteria
#6
Oscar I Bernal, Bhuvnesh Bharti, Michael C Flickinger, Orlin D Velev
We report how dielectrophoresis (DEP) can be used as a tool for the fabrication of biocomposite coatings of photoreactive cyanobacteria (Synechococcus PCC7002) on flexible polyester sheets (PEs). The PE substrates were precoated by a layer-by-layer assembled film of charged polyelectrolytes. In excellent agreement between experimental data and numerical simulations, the directed assembly process driven by external electric field results in the formation of 1D chains and 2D sheets by the cells. The preassembled cyanobacteria chains and arrays became deposited on the substrate and remained in place after the electric field was turned off due to the electrostatic attraction between the negatively charged cell surfaces and the positively charged polyelectrolyte-coated PE...
May 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28481525/spatial-manipulation-and-assembly-of-nanoparticles-by-atomic-force-microscopy-tip-induced-dielectrophoresis
#7
Peilin Zhou, Haibo Yu, Wenguang Yang, Yangdong Wen, Zhidong Wang, Wen Jung Li, Lianqing Liu
In this article, we present a novel method of spatial manipulation and assembly of nanoparticles via atomic force microscopy tip-induced dielectrophoresis (AFM-DEP). This method combines the high-accuracy positioning of AFM with the parallel manipulation of DEP. A spatially nonuniform electric field is induced by applying an alternating current (AC) voltage between the conductive AFM probe and an indium tin oxide glass substrate. The AFM probe acted as a movable DEP tweezer for nanomanipulation and assembly of nanoparticles...
May 8, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28481265/characterizing-esophageal-cancerous-cells-at-different-stages-using-the-dielectrophoretic-impedance-measurement-method-in-a-microchip
#8
Hsiang-Chen Wang, Ngoc-Viet Nguyen, Rui-Yi Lin, Chun-Ping Jen
Analysis of cancerous cells allows us to provide useful information for the early diagnosis of cancer and to monitor treatment progress. An approach based on electrical principles has recently become an attractive technique. This study presents a microdevice that utilizes a dielectrophoretic impedance measurement method for the identification of cancerous cells. The proposed biochip consists of circle-on-line microelectrodes that are patterned using a standard microfabrication processes. A sample of various cell concentrations was introduced in an open-top microchamber...
May 6, 2017: Sensors
https://www.readbyqxmd.com/read/28476994/fabrication-challenges-and-perspectives-on-the-use-of-carbon-electrode-dielectrophoresis-in-sample-preparation
#9
REVIEW
Rodrigo Martinez-Duarte
The focus of this review is to assess the current status of three-dimensional (3D) carbon-electrode dielectrophoresis (carbonDEP) and identify the challenges currently preventing it from its use in high-throughput applications such as sample preparation for diagnostics. The use of 3D electrodes over more traditional planar ones is emphasised here as a way to increase the throughput of DEP devices. Glass-like carbon electrodes are derived through the carbonisation of photoresist structures made using photolithography...
March 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28475301/label-free-quantification-of-intracellular-mitochondrial-dynamics-using-dielectrophoresis
#10
Ali Rohani, John H Moore, Jennifer A Kashatus, Hiromi Sesaki, David F Kashatus, Nathan S Swami
Mitochondrial dynamics play an important role within several pathological conditions, including cancer and neurological diseases. For the purpose of identifying therapies that target aberrant regulation of the mitochondrial dynamics machinery and characterizing the regulating signaling pathways, there is a need for label-free means to detect the dynamic alterations in mitochondrial morphology. We present the use of dielectrophoresis for label-free quantification of intracellular mitochondrial modifications that alter cytoplasmic conductivity, and these changes are benchmarked against label-based image analysis of the mitochondrial network...
May 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28467599/editorial-dielectrophoresis-2017
#11
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
May 3, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28467596/assessing-the-importance-of-the-root-mean-square-rms-value-of-different-waveforms-to-determine-the-strength-of-a-dielectrophoresis-trapping-force
#12
Jordon Gilmore, Monsur Islam, Josie Duncan, Rucha Natu, Rodrigo Martinez-Duarte
No abstract text is available yet for this article.
May 3, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28465513/a-novel-microfluidic-3d-platform-for-culturing-pancreatic-ductal-adenocarcinoma-cells-comparison-with-in-vitro-cultures-and-in-vivo-xenografts
#13
Meike Beer, Nirmala Kuppalu, Matteo Stefanini, Holger Becker, Ingo Schulz, Sagar Manoli, Julia Schuette, Christian Schmees, Armando Casazza, Martin Stelzle, Annarosa Arcangeli
The integration of microfluidics and cell biology has reached a significant milestone with the development of "organ-on-chips", smart technological platforms that, once applied to the study of human diseases, such as cancer, might ultimately contribute to design personalised treatments and hence improve health outcomes. This paper reports that the combination of microfluidics and dielectrophoresis (DEP) allows to culture different pancreatic ductal adenocarcinoma (PDAC) human cell lines into a cyclic olefin polymer (COP) chamber (HepaChip(®)), enriched by the extracellular matrix (ECM) protein collagen...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28464521/on-chip-spectroscopic-assessment-of-microbial-susceptibility-to-antibiotics-within-3-5%C3%A2-hours
#14
Ulrich-Christian Schröder, Johanna Kirchhoff, Uwe Hübner, Günter Mayer, Uwe Glaser, Thomas Henkel, Wolfgang Pfister, Wolfgang Fritzsche, Jürgen Popp, Ute Neugebauer
In times of rising antibiotic resistances, there is a high need for fast, sensitive and specific methods to determine antibiotic susceptibilities of bacterial pathogens. Here, we present an integrated microfluidic device in which bacteria from diluted suspensions are captured in well-defined regions using on-chip dielectrophoresis and further analyzed in a label-free and non-destructive manner using Raman spectroscopy. Minimal sample preparation and automated sample processing ensure safe handling of infectious material with minimal hands-on time for the operator...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28453962/a-bead-based-immunofluorescence-assay-on-a-microfluidic-dielectrophoresis-platform-for-rapid-dengue-virus-detection
#15
Edwar Iswardy, Tien-Chun Tsai, I-Fang Cheng, Tzu-Chuan Ho, Guey Chuen Perng, Hsien-Chang Chang
The proof of concept of utilizing a microfluidic dielectrophoresis (DEP) chip was conducted to rapidly detect a dengue virus (DENV) in vitro based on the fluorescence immunosensing. The mechanism of detection was that the DEP force was employed to capture the modified beads (mouse anti-flavivirus monoclonal antibody-coated beads) in the microfluidic chip and the DENV modified with fluorescence label, as the detection target, can be then captured on the modified beads by immunoreaction. The fluorescent signal was then obtained through fluorescence microscopy, and then quantified by ImageJ freeware...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28434715/separation-of-janus-droplets-and-oil-droplets-in-microchannels-by-wall-induced-dielectrophoresis
#16
Mengqi Li, Dongqing Li
The continuous separation of different types of droplets from a mixture is important in industrial and research applications. Currently researches for droplet separation focus on homogeneous emulsion droplets, and there is no study on the separation of heterogeneous droplets. The wall-induced dielectrophoresis, which originates from the non-uniform electrical field around a sphere nearby a planar wall, can be applied to separate dielectric particle and cells. In this work, the continuous separations of oil droplets and the electrically induced Janus droplets (EIJDs) in a microchannel by using the wall-induced dielectrophoresis method were presented...
April 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28432530/an-integrated-microsystem-with-dielectrophoresis-enrichment-and-impedance-detection-for-detection-of-escherichia-coli
#17
Renjie Wang, Yi Xu, Haitao Liu, Jinlan Peng, Joseph Irudayaraj, Feiyun Cui
An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10(-3) m(3), and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28429819/effects-of-dielectrophoresis-on-thrombogenesis-in-human-whole-blood
#18
Steven Kinio, James K Mills
Thrombogenesis (blood clot formation) is a major barrier to the development of biomedical devices that interface with blood. Although state-of-the-art chemically and pharmacologically mediated clot mitigation strategies are effective, some limitations of such approaches include depletion of active agents, or adverse reactions in patients. Increased clotting protein adsorption and platelet adhesion, which occur when artificial surfaces are exposed to blood result in enhanced clot formation on artificial surfaces...
April 21, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28417250/impact-of-surface-roughness-on-dielectrophoretically-assisted-concentration-of-microorganisms-over-pcb-based-platforms
#19
Geeta Bhatt, Rishi Kant, Keerti Mishra, Kuldeep Yadav, Deepak Singh, Ramanathan Gurunath, Shantanu Bhattacharya
This article presents a PCB based microfluidic platform for performing a dielectrophoretic capture of live microorganisms over inter-digitated electrodes buried under layers of different surface roughness values. Although dielectrophoresis has been extensively studied earlier over silicon and polymer surfaces with printed electrodes the issue of surface roughness particularly in case of buried electrodes has been seldom investigated. We have addressed this issue through a layer of spin coated PDMS (of various surface roughness) that is used to cover the printed electrodes over a printed circuit board...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28408395/high-throughput-low-loss-low-cost-and-label-free-cell-separation-using-electrophysiology-activated-cell-enrichment
#20
Shabnam A Faraghat, Kai F Hoettges, Max K Steinbach, Daan R van der Veen, William J Brackenbury, Erin A Henslee, Fatima H Labeed, Michael P Hughes
Currently, cell separation occurs almost exclusively by density gradient methods and by fluorescence- and magnetic-activated cell sorting (FACS/MACS). These variously suffer from lack of specificity, high cell loss, use of labels, and high capital/operating cost. We present a dielectrophoresis (DEP)-based cell-separation method, using 3D electrodes on a low-cost disposable chip; one cell type is allowed to pass through the chip whereas the other is retained and subsequently recovered. The method advances usability and throughput of DEP separation by orders of magnitude in throughput, efficiency, purity, recovery (cells arriving in the correct output fraction), cell losses (those which are unaccounted for at the end of the separation), and cost...
May 2, 2017: Proceedings of the National Academy of Sciences of the United States of America
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