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dielectrophoresis

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https://www.readbyqxmd.com/read/29134412/engineered-bone-scaffolds-with-dielectrophoresis-based-patterning-using-3d-printing
#1
Zhijie Huan, Henry K Chu, Hongbo Liu, Jie Yang, Dong Sun
Patterning of cells into a specific pattern is an important procedure in tissue engineering to facilitate tissue culture and ingrowth. In this paper, a new type of 3D-printed scaffold utilizing dielectrophoresis (DEP) for active cell seeding and patterning was proposed. This scaffold adopted a concentric-ring design that is similar to native bone tissues. The scaffold was fabricated with a commercial three-dimensional (3D) printer. Polylactic Acid (PLA) was selected as the material for the printer and the fabricated scaffold was coated with gold to enhance the conductivity for DEP manipulation...
November 13, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29131586/single-walled-carbon-nanotubes-probed-with-insulator-based-dielectrophoresis
#2
Mohammad Towshif Rabbani, Christoph F Schmidt, Alexandra Ros
Single-walled carbon nanotubes (SWNTs) offer unique electrical and optical properties. Common synthesis processes yield SWNTs with large length polydispersity (several tens of nanometers up to centimeters) and heterogeneous electrical and optical properties. Applications often require suitable selection and purification. Dielectrophoresis is one manipulation method for separating SWNTs based on dielectric properties and geometry. Here, we present a study of surfactant and ssDNA wrapped SWNTs suspended in aqueous solutions manipulated by insulator-based dielectrophoresis (iDEP)...
November 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29130902/dielectrophoresis-based-multi-step-nanowire-assembly-on-a-flexible-superstrate
#3
Xin Wang, Ke Chen, Linbo Liu, Nan Xiang, Zhonghua Ni
Abstract Nanowire assembly based on dielectrophoresis (DEP) could be a useful and efficient tool for fabricating nanowire-based devices. Although there have been extensive reports on the DEP nanowire assembly, the new approaches that make DEP more facile and affordable are still desirable. Herein, we present an approach using the reusable electrodes to assemble silver nanowires onto a removable, independent polyethylene terephthalate (PET) film. The PET film is placed on the reusable electrodes, and a sinusoidal AC voltage is applied to the electrodes to induce DEP force for nanowire assembly upon the flexible film...
November 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29130508/review-electric-field-driven-pumping-in-microfluidic-device
#4
REVIEW
Mohammad R Hossan, Diganta Dutta, Nazmul Islam, Prashanta Dutta
Pumping of fluids with precise control is one of the key components in a microfluidic device. The electric field has been used as one of the most popular and efficient nonmechanical pumping mechanism to transport fluids in microchannels from the very early stage of microfluidic technology development. This review presents fundamental physics and theories of the different microscale phenomena that arise due to the application of an electric field in fluids, which can be applied for pumping of fluids in microdevices...
November 11, 2017: Electrophoresis
https://www.readbyqxmd.com/read/29119187/reaching-for-the-limits-in-continuous-flow-dielectrophoretic-dna-analysis
#5
Sarah Täuber, Lena Kunze, Oleg Grauberger, Armin Grundmann, Martina Viefhues
The efficient purification and analysis of topological DNA variants is mandatory for many state-of-the-art molecular medicine technologies, like gene- and cancer-therapy as well as plasmid vaccination. In this work, we exploit dielectrophoresis (DEP) for a fast and efficient continuous-flow separation and analysis that goes beyond the standard methods of gel electrophoresis and capillary electrophoresis. The aim of this work was to reach for the limits in dielectrophoretic analysis of DNA regarding the size resolution and the topological conformation...
November 9, 2017: Analyst
https://www.readbyqxmd.com/read/29115319/a-planar-dielectrophoresis-based-chip-for-high-throughput-cell-pairing
#6
ChunHui Wu, RiFei Chen, Yu Liu, ZhenMing Yu, YouWei Jiang, Xing Cheng
This paper reports the design and fabrication of a planar chip for high-throughput cell trapping and pairing (more than 2400 single cell-cell pairs in a microwell array) in a 1 × 1.5 cm area by positive dielectrophoresis (p-DEP) within only several minutes. The p-DEP was generated by applying an alternating current signal on a novel two-pair interdigitated array (TPIDA) electrode. The TPIDA electrode not only enabled the planar chip to be incorporated with a most often used PDMS microfluidic channel, but also contributed to a high single cell-cell pairing efficiency up to 74...
November 8, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29106427/micro-nanoelectrochemical-probe-and-chip-devices-for-evaluation-of-three-dimensional-cultured-cells
#7
Kosuke Ino, Mustafa Şen, Hitoshi Shiku, Tomokazu Matsue
Herein, we present an overview of recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells. First, we discuss probe devices: a general outline, evaluation of O2 consumption, enzyme-modified electrodes, evaluation of endogenous enzyme activity, and the collection of cell components from cell aggregates are discussed. The next section is focused on integrated chip devices: a general outline, electrode array devices, smart electrode array devices, droplet detection of 3D cultured cells, cell manipulation using dielectrophoresis (DEP), and electrodeposited hydrogels used for fabrication of 3D cultured cells on chip devices are discussed...
November 6, 2017: Analyst
https://www.readbyqxmd.com/read/29082438/dielectrophoretic-microbead-sorting-using-modular-electrode-design-and-capillary-driven-microfluidics
#8
Jaione Tirapu-Azpiroz, Yuksel Temiz, Emmanuel Delamarche
Multiplexing assays using microbeads in microfluidics offers high flexibility and throughput, but requires the ability to sort particles based on their physical properties. In this paper, we present a continuous method for separating microbeads that is compact, modular and adaptive, employing an optimized electrode layout that alternates sorting and concentration of microbeads using dielectrophoresis and a nested design. By simulating the combined effects of the hydrodynamic drag and dielectrophoresis forces on polystyrene beads, the parameters of the electrode layout and voltage configuration are optimized for maximum separation based on particle size with a small number of slanted planar electrodes...
October 30, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29081637/in-situ-poling-and-structurization-of-piezoelectric-particulate-composites
#9
H Khanbareh, S van der Zwaag, W A Groen
Composites of lead zirconate titanate particles in an epoxy matrix are prepared in the form of 0-3 and quasi 1-3 with different ceramic volume contents from 10% to 50%. Two different processing routes are tested. Firstly a conventional dielectrophoretic structuring is used to induce a chain-like particle configuration, followed by curing the matrix and poling at a high temperature and under a high voltage. Secondly a simultaneous combination of dielectrophoresis and poling is applied at room temperature while the polymer is in the liquid state followed by subsequent curing...
November 2017: Journal of Intelligent Material Systems and Structures
https://www.readbyqxmd.com/read/29068385/solution-based-methods-for-the-fabrication-of-carbon-nanotube-modified-atomic-force-microscopy-probes
#10
Ashley D Slattery, Cameron J Shearer, Joseph G Shapter, Jamie S Quinton, Christopher T Gibson
High aspect ratio carbon nanotubes are ideal candidates to improve the resolution and lifetime of atomic force microscopy (AFM) probes. Here, we present simple methods for the preparation of carbon nanotube modified AFM probes utilising solvent evaporation or dielectrophoresis. Scanning electron microscopy (SEM) of the modified probes shows that the carbon nanotubes attach to the probe apex as fibres and display a high aspect ratio. Many of the probes made in this manner were initially found to exhibit anomalous feedback characteristics during scanning, which rendered them unsuitable for imaging...
October 25, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29068080/electrothermal-enrichment-of-submicron-particles-in-an-insulator-based-dielectrophoretic-microdevice
#11
Akshay Kale, Le Song, Xinyu Lu, Liandong Yu, Guoqing Hu, Xiangchun Xuan
Insulator-based dielectrophoresis (iDEP) exploits in-channel hurdles and posts etc. to create electric field gradients for various particle manipulations. However, the presence of such insulating structures also amplifies the Joule heating in the fluid around themselves, leading to both temperature gradients and electrothermal flow. These Joule heating effects have been previously demonstrated to weaken the dielectrophoretic focusing and trapping of microscale and nanoscale particles. We find that the electrothermal flow vortices are able to entrain submicron particles for a localized enrichment near the insulating tips of a ratchet microchannel...
October 25, 2017: Electrophoresis
https://www.readbyqxmd.com/read/29065488/few-flakes-reduced-graphene-oxide-sensors-for-organic-vapors-with-a-high-signal-to-noise-ratio
#12
Nowzesh Hasan, Wenli Zhang, Adarsh D Radadia
This paper reports our findings on how to prepare a graphene oxide-based gas sensor for sensing fast pulses of volatile organic compounds with a better signal-to-noise ratio. We use rapid acetone pulses of varying concentrations to test the sensors. First, we compare the effect of graphene oxide deposition method (dielectrophoresis versus solvent evaporation) on the sensor's response. We find that dielectrophoresis yields films with uniform coverage and better sensor response. Second, we examine the effect of chemical reduction...
October 21, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29060674/analysis-of-dielectrophoresis-based-3d-focusing-in-microfluidic-devices-with-planar-electrodes
#13
Ali Hilal-Alnaqbi, Anas Alazzam, Sawsan Dagher, Bobby Mathew
This article models a dielectrophoresis based approach for achieving 3D focusing, of micro-scale objects, in microfluidic devices. The microfluidic device employs four planar electrodes; two electrodes each on the top and bottom surface of the microchannel and each slightly protrude into the microchannel. Each electrode establishes electric field with the neighboring electrode on the same and opposite surfaces. The dielectrophoretic force pushes the micro-scale objects both the directions transverse to the flow direction to achieve the desired 3D focusing...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29060673/determination-of-red-blood-cell-fatigue-using-electrodeformation
#14
A Amirouche, M Faivre, J F Chateaux, R Ferrigno
In this work, we used electrodeformation (ED) [1] as a new strategy to evaluate the fatigue of healthy human Red Blood Cells (RBCs). Using dielectrophoresis (DEP) forces, we submitted RBCs to a series of elongation and relaxation cycles to model their mechanical stress in the blood circulation and we used their relaxation time as a marker to evaluate their fatigue. In this paper, we first investigated the dependency of the RBC mechanical response upon the experimental parameters, such as the viscosity ηout of the external medium, the amplitude ΔV of the applied voltage, the duration of the solicitation tsol, the number of solicitations N as well as the resting time trest between two solicitations...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29053456/localized-electroporation-with-dielectrophoretic-field-flow-fractionation-towards-removal-of-circulating-tumour-cells-from-human-blood
#15
Steven Kinio, James K Mills
This paper presents the design and experimental performance of a microelectrode-based device to selectively lyse cells in a flow in a microfluidic channel. Localized cell lysis is achieved by utilizing "irreversible electroporation", in which cells are exposed to high magnitude electric pulses. Localized cell lysis in a flow has research applications and may allow for removal of harmful cells such as circulating tumor cells from blood. Due to the dependence of this technique on the magnitude of the applied electric field, lethal electric field regions can be localized in the channel by calibration of the applied voltage...
October 17, 2017: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/29027906/directed-assembly-based-printing-of-homogeneous-and-hybrid-nanorods-using-dielectrophoresis
#16
Zhimin Chai, Cihan Yilmaz, Ahmed Busnaina, Charles A Lissandrello, David J D Carter
Printing nano and microscale three-dimensional (3D) structures using directed assembly of nanoparticles has many potential applications in electronics, photonics and biotechnology. This paper presents a reproducible and scalable 3D dielectrophoresis assembly process for printing homogeneous silica and hybrid silica/gold nanorods from silica and gold nanoparticles. The nanoparticles are assembled into patterned vias under a dielectrophoretic force generated by an alternating current (AC) field, and then completely fused in situ to form nanorods...
October 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29019239/enhanced-dc-operated-electroluminescence-of-forwardly-aligned-%C3%A2-%C3%A2-p-mqw-n-ingan-nanorod-leds-via-dc-offset-ac-dielectrophoresis
#17
Yun Jae Eo, Gang Yeol Yoo, Hyelim Kang, Young Ki Lee, Chan Sik Kim, Ji Hye Oh, Keyong Nam Lee, Woong Kim, Young Rag Do
We introduce an orientation-controlled alignment process of p-GaN/InGaN multiquantum-well/n-GaN (p/MQW/n InGaN) nanorod light-emitting diodes (LEDs) by applying the direct current (DC) offset-alternating current (AC) or pulsed DC electric fields across interdigitated metal electrodes. The as-forwardly aligned p/MQW/n InGaN nanorod LEDs by a pulsed DC dielectrophoresis (DEP) assembly process improve the electroluminescence (EL) intensities by 1.8 times compared to the conventional AC DEP assembly process under DC electric field operation and exhibit an enhanced applied current and EL brightness in the current-voltage and EL intensity-voltage curves, which can be directly used as the fundamental data to construct DC-operated nanorod LED devices, such as LED areal surface lightings, scalable lightings (micrometers to inches) and formable surface lightings...
October 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28978446/determination-of-cell-membrane-capacitance-and-conductance-via-optically-induced-electrokinetics
#18
Wenfeng Liang, Yuliang Zhao, Lianqing Liu, Yuechao Wang, Wen Jung Li, Gwo-Bin Lee
Cell membrane capacitance and conductance are key pieces of intrinsic information correlated with the cellular dielectric parameters and morphology of the plasma membrane; these parameters have been used as electrophysiological biomarkers to characterize cellular phenotype and state, and they have many associated clinical applications. Here, we present our work on the non-invasive determination of cell membrane capacitance and conductance by an optically activated microfluidics chip. The model for determining the cell membrane capacitance and conductance was established by a single layer of the shell-core polarization model...
October 3, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28975645/effects-of-electrothermal-vortices-on-insulator-based-dielectrophoresis-for-circulating-tumor-cell-separation
#19
Arian Aghilinejad, Mohammad Aghaamoo, Xiaolin Chen, Jie Xu
Insulator-based dielectrophoresis (iDEP) is a powerful technique for separation and manipulation of bioparticles. In recent years, iDEP designs using arrays of insulating posts have shown promising results toward reaching high-efficiency bioparticle manipulation. Joule heating (JH) and electrothermal (ET) flows have been observed in iDEP microdevices and significantly affecting their performances. In this research, we utilize mathematical modeling to study, iDEP technique and the effects of JH and ET flow on device performance and propose a separation scenario for selective trapping of circulating tumor cells (CTCs)...
October 4, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28974001/comparison-of-sensitivity-and-quantitation-between-microbead-dielectrophoresis-based-dna-detection-and-real-time-pcr
#20
Michihiko Nakano, Zhenhao Ding, Junya Suehiro
In this study, we describe a microbead-based method using dielectrophoresis (DEP) for the fast detection of DNA amplified by polymerase chain reaction (PCR). This electrical method measures the change in impedance caused by DEP-trapped microbeads to which biotinylated target DNA molecules are chemically attached. Using this method, measurements can be obtained within 20 min. Currently, real-time PCR is among the most sensitive methods available for the detection of target DNA, and is often used in the diagnosis of infectious diseases...
September 30, 2017: Biosensors
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