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https://www.readbyqxmd.com/read/29321565/characterization-of-novel-endo-%C3%AE-n-acetylglucosaminidases-from-sphingobacterium-species-beauveria-bassiana-and-cordyceps-militaris-that-specifically-hydrolyze-fucose-containing-oligosaccharides-and-human-igg
#1
Yibo Huang, Yujiro Higuchi, Takashi Kinoshita, Ai Mitani, Yasunari Eshima, Kaoru Takegawa
Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl chloride (Dns)-labeled sialylglycan (Dns-SG) as a substrate, and discovered a strain that exhibits ENGase activity in the culture supernatant; this strain, named here as strain HMA12, was identified as a Sphingobacterium species by 16S ribosomal RNA gene analysis...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29303996/pichia-pastoris-is-a-suitable-host-for-the-heterologous-expression-of-predicted-class-i-and-class-ii-hydrophobins-for-discovery-study-and-application-in-biotechnology
#2
Julie-Anne Gandier, Emma R Master
The heterologous expression of proteins is often a crucial first step in not only investigating their function, but also in their industrial application. The functional assembly and aggregation of hydrophobins offers intriguing biotechnological applications from surface modification to drug delivery, yet make developing systems for their heterologous expression challenging. In this article, we describe the development of Pichia pastoris KM71H strains capable of solubly producing the full set of predicted Cordyceps militaris hydrophobins CMil1 (Class IA), CMil2 (Class II), and CMil3 (IM) at mg/L yields with the use of 6His-tags not only for purification but for their detection...
January 5, 2018: Microorganisms
https://www.readbyqxmd.com/read/29243433/-comparative-study-on-specific-chromatograms-and-main-nucleosides-of-cultivated-and-wild-cordyceps-sinensis
#3
Ke Zan, Li-Li Huang, Li-Nong Guo, Jie Liu, Jian Zheng, Shuang-Cheng Ma, Zheng-Ming Qian, Wen-Jia Li
This study is to establish the HPLC specific chromatogram and determine four main nucleosides of wild and cultivated Cordyceps sinensis. Uridine, inosine, guanosine and adenosine were selected as reference substance. HPLC analysis was performed on a Waters XSelect HSS T3 C₁₈ (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of water(A)-acetonitrile (B) at a flow rate of 0.6 mL•min⁻¹ (0-5 min,0% B;5-15 min,0%-10% B, 15-30 min,10%-20% B, 30-33 min, 20%-50% B, 33-35 min, 50%-0% B, 35-40 min, 0% B)...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29242779/a-phylogenetically-based-nomenclature-for-cordycipitaceae-hypocreales
#4
Ryan M Kepler, J Jennifer Luangsa-Ard, Nigel L Hywel-Jones, C Alisha Quandt, Gi-Ho Sung, Stephen A Rehner, M Catherine Aime, Terry W Henkel, Tatiana Sanjuan, Rasoul Zare, Mingjun Chen, Zhengzhi Li, Amy Y Rossman, Joseph W Spatafora, Bhushan Shrestha
The ending of dual nomenclatural systems for pleomorphic fungi in 2011 requires the reconciliation of competing names, ideally linked through culture based or molecular methods. The phylogenetic systematics of Hypocreales and its many genera have received extensive study in the last two decades, however resolution of competing names in Cordycipitaceae has not yet been addressed. Here we present a molecular phylogenetic investigation of Cordycipitaceae that enables identification of competing names in this family, and provides the basis upon which these names can be maintained or suppressed...
December 2017: IMA Fungus
https://www.readbyqxmd.com/read/29212184/cordycepin-and-a-preparation-from-cordyceps-militaris-inhibit-malignant-transformation-and-proliferation-by-decreasing-egfr-and-il-17ra-signaling-in-a-murine-oral-cancer-model
#5
Peng-Yang Hsu, Yueh-Hsin Lin, Erh-Ling Yeh, Hui-Chen Lo, Tai-Hao Hsu, Che-Chun Su
Cordyceps militaris (CM) and its active ingredient cordycepin have been reported to inhibit tumor growth, but the mechanisms are not fully understood. This study used a mouse model for oral cancer and a cell line, 4NAOC-1 derived from the model to study the mechanisms. Our results show that a CM preparation (CMP) can significantly inhibit tumor development and malignant transformation in the model. In vitro data indicate that CMP and cordycepin can inhibit 4NAOC-1 cell proliferation, either anchorage-dependent or -independent...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29199566/clinical-effects-of-a-commercial-supplement-of-ophiocordyceps-sinensis-and-ganoderma-lucidum-on-cognitive-function-of-healthy-young-volunteers
#6
Sharon Tsuk, Yarden Har Lev, Arie Rotstein, Rafi Carasso, Aviva Zeev, Yael Netz, Tzvi Dwolatski, Gilit Steiner
Ophiocordyceps sinensis (= Cordyceps sinensis) and Ganoderma lucidum are medicinal mushrooms used in traditional Chinese medicine. The effects of O. sinensis and G. lucidum on cognitive function have been evaluated through the use of animal models and in vitro studies, which indicated beneficial effects. The purpose of this study was to evaluate the effects of treatment with a commercially available supplement of O. sinensis and G. lucidum on cognitive function in young, healthy human participants. Physical education students (n = 96 [53 men, 43 women]; mean ± standard deviation age, 26...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29199564/effect-of-pulsed-light-irradiation-on-bioactive-nonvolatile-components-and-antioxidant-properties-of-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes
#7
Shih-Jeng Huang, Fu-Kuei Huang, Agnes Purwidyantri, Arif Rahmandita, Shu-Yao Tsai
The caterpillar medicinal mushroom Cordyceps militaris contains many bioactive components, such as adenosine, cordycepin, and polysaccharides. In this study, C. militaris was exposed to 0, 3, 6, or 9 pulses of light irradiation to estimate changes in vitamin D2, bioactive compounds, nonvolatile taste components, and antioxidant properties. In addition, we compared the components and properties of C. militaris mycelia and solid waste medium that had been treated with pulsed light (PL) irradiation. Overall, PL irradiation of C...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29187652/-cordyceps-sinensis-protects-hk2-cells-from-ischemia-reperfusion-injury-through-sirt1-pathway
#8
Yingli Zhang, Xiang Ao, Hui Li, Songyun Deng, Zhou Xiao, Weisheng Peng, Jinhua Xiang, Qiaoling Zhou
To investigate the effects of Cordyceps sinensis (CS) on cellular apoptosis and Sirt1 expression in HK2 cells followed by ischemia-reperfusion (I/R).
 Methods: HK2 cells were incubated with different concentrations of CS (10, 20, 40, 80, 160, 320 mg/L) for 24 hours, and the optimal concentration of CS was selected by measuring cell proliferation. The confluent HK2 cells were incubated with 0.01 μmol/L antimycin A for 2 hours to induce ischemia in vitro, and then the reperfusion was achieved by incubating cells with glucose-replete complete growth medium for 24 hours...
November 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/29178836/chromosome-level-assembly-and-secondary-metabolite-potential-of-the-parasitic-fungus-cordyceps-militaris
#9
Glenna J Kramer, Justin R Nodwell
BACKGROUND: Cordyceps militaris is an insect pathogenic fungus that is prized for its use in traditional medicine. This and other entomopathogenic fungi are understudied sources for the discovery of new bioactive molecules. In this study, PacBio SMRT long read sequencing technology was used to sequence the genome of C. militaris with a focus on the genetic potential for secondary metabolite production in the genome assembly of this fungus. RESULTS: This is first chromosome level assembly of a species in the Cordyceps genera...
November 25, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29168868/structural-elucidation-and-immunostimulatory-activity-of-a-new-polysaccharide-from-cordyceps-militaris
#10
Sixue Bi, Yongshuai Jing, Qinqin Zhou, Xianjing Hu, Jianhua Zhu, Zhongyi Guo, Liyan Song, Rongmin Yu
A new polysaccharide (CMPB90-1) was isolated from cultured Cordyceps militaris by alkaline extraction. The chemical structure of CMPB90-1 was determined by analysis of physicochemical and spectral data. The backbone of CMPB90-1 is composed of (1→6)-linked α-d-glucopyranosyl and (1→3)-linked α-d-glucopyranosyl residues, with branching at O-6, which consists of (1→4)-linked β-d-mannopyranosyl and (1→6)-linked α-d-glucopyranosyl residues, respectively. β-d-Galactopyranosyl residues is the terminal unit...
November 23, 2017: Food & Function
https://www.readbyqxmd.com/read/29168246/immunostimulatory-effects-of-cordycepin-enriched-wib-801ce-from-cordyceps-militaris-in-splenocytes-and-cyclophosphamide-induced-immunosuppressed-mice
#11
Ji-Sun Shin, Soo-Hak Chung, Woo-Seok Lee, Je-Young Lee, Jong-Lae Kim, Kyung-Tae Lee
The medicinal mushroom Cordyceps militaris has been reported to possess anticancer and immunomodulatory effects. We investigated the immunostimulatory effects of culture supernatant of C. militaris (WIB-801CE) by examining its in vitro enhancing effects on cell proliferation and cytokine releases in splenocytes and its in vivo effects on cyclophosphamide-induced immunosuppressed mice. WIB-801CE enhanced normal and methotrexate-induced cell proliferation. WIB-801CE significantly ameliorated interleukin (IL)-2, interferon-γ, and tumor necrosis factor-α secretion in methotrexate-induced splenocytes...
November 23, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29161660/molecular-mechanisms-of-anti-inflammatory-effect-of-chrysophanol-an-active-component-of-ast2017-01-on-atopic-dermatitis-in-vitro-models
#12
Hyun-Ja Jeong, Hee-Yun Kim, Hyung-Min Kim
AST2017-01 mainly consists of Rumex crispus and -Cordyceps militaris and has been widely consumed as an herbal medicine or functional food in Korea. Here we investigated the influences of AST2017-01 and its active component, chrysophanol on human mast cell (HMC-1 cell) and human keratinocyte (HaCaT cell)-mediated inflammatory reactions. Pretreatment with AST2017-01 or chrysophanol suppressed intracellular calcium levels and histamine release in phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-treated HMC-1 cells...
November 18, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29157818/biological-characteristics-bioactive-components-and-antineoplastic-properties-of-sporoderm-broken-spores-from-wild-cordyceps-cicadae
#13
Yanfang Sun, Michael Wink, Pan Wang, Hongfei Lu, Hongxin Zhao, Hongtao Liu, Shixian Wang, Yang Sun, Zongsuo Liang
BACKGROUND: Cordyceps cicadae, an entomogenous fungus has been used as a dietary therapeutic in traditional Chinese medicine for several millennia, in the form of powders and decoction. However, wild C. cicadae is notably scarce. To date, there is still a lack of comprehensive and deep studies on the biological characteristics, chemical profiles and antineoplastic mechanisms of C. cicadae, especially its spores. AIM OF THE STUDY: This study aimed to identify wild C...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29139260/-study-on-distribution-of-five-heavy-metal-elements-in-different-parts-of-cordyceps-sinensis-by-microwave-digestion-icp-ms
#14
Li Zhou, Qing-Xiu Hao, Sheng Wang, Quan Yang, Chuan-Zhi Kang, Wan-Zhen Yang, Lan-Ping Guo
The contents of five heavy metals (Cu, Pb, As, Cd, Hg) in 17 batches of Cordyceps sinensis were determined by microwave digestion-ICP-MS, and their distribution in C. sinensis were analyzed. The results showed that the contents of Cu, Pb, Cd and Hg in all batches were in accordance with the international standards of Chinese Medicine-Chinese Herbal Medicine Heavy Metal Limit, with their contents in the stroma higher than that in the caterpillar body, and the excess rate of As, which mainly concentrated in the caterpillar body part of C...
August 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29130448/a-review-on-the-effects-of-current-chemotherapy-drugs-and-natural-agents-in-treating-non-small-cell-lung-cancer
#15
Chih-Yang Huang, Da-Tong Ju, Chih-Fen Chang, P Muralidhar Reddy, Bharath Kumar Velmurugan
Lung cancer is the leading cause of cancer deaths worldwide, and this makes it an attractive disease to review and possibly improve therapeutic treatment options. Surgery, radiation, chemotherapy, targeted treatments, and immunotherapy separate or in combination are commonly used to treat lung cancer. However, these treatment types may cause different side effects, and chemotherapy-based regimens appear to have reached a therapeutic plateau. Hence, effective, better-tolerated treatments are needed to address and hopefully overcome this conundrum...
December 2017: BioMedicine
https://www.readbyqxmd.com/read/29126983/yeast-like-microorganisms-in-the-scale-insect-kermes-quercus-insecta-hemiptera-coccomorpha-kermesidae-newly-acquired-symbionts
#16
Elżbieta Podsiadło, Katarzyna Michalik, Anna Michalik, Teresa Szklarzewicz
Scale insects, like other plant sap-consumers, are host to symbiotic microorganisms which provide them with the substances missing from their diet. In contrast to most scale insects, Kermes quercus (Linnaeus) was regarded as asymbiotic. Our histological and ultrastructural observations show that in the body of the feeding stages of K. quercus collected in two locations (Warsaw and Cracow), numerous yeast-like microorganisms occur. These microorganisms were localized in the cytoplasm of fat body cells. The yeast-like microorganisms were observed neither in other organs of host insect nor in the eggs...
November 7, 2017: Arthropod Structure & Development
https://www.readbyqxmd.com/read/29124970/a-review-on-the-nucleic-acid-constituents-in-mushrooms-nucleobases-nucleosides-and-nucleotides
#17
Chia-Wei Phan, Joon-Keong Wang, Shiau-Chuen Cheah, Murali Naidu, Pamela David, Vikineswary Sabaratnam
Mushrooms have become increasingly important as a reliable food source. They have also been recognized as an important source of bioactive compounds of high nutritional and medicinal values. The nucleobases, nucleosides and nucleotides found in mushrooms play important roles in the regulation of various physiological processes in the human body via the purinergic and/or pyrimidine receptors. Cordycepin, a 3'-deoxyadenosine found in Cordyceps sinensis has received much attention as it possesses many medicinal values including anticancer properties...
November 10, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29113024/neuroprotective-effects-of-butanol-fraction-of-cordyceps-cicadae-on-glutamate-induced-damage-in-pc12-cells-involving-oxidative-toxicity
#18
Dan Wang, Jibiao Wang, Dujun Wang, Xiaofeng Yu, Opeyemi Joshua Olatunji, Zhen Ouyang, Yuan Wei
The current study was aimed at investigating the neuroprotective effects of the butanol fraction from C. cicadae (CBU ), which was responsible for the anti-aging effect of this medicine. Glutamate-induced PC12 cells were used as a model to determine the neuroprotective effect against oxidative cell death. Cell viability, cytotoxicity, flow cytometry, mitochondrial transmembrane potential (MMP), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels were analyzed to assess neuronal cell survival or death...
November 7, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29091925/insight-into-cordycepin-biosynthesis-of-cordyceps-militaris-comparison-between-a-liquid-surface-culture-and-a-submerged-culture-through-transcriptomic-analysis
#19
Ahmad Suparmin, Tatsuya Kato, Hideo Dohra, Enoch Y Park
Cordyceps militaris produces cordycepin, which is known to be a bioactive compound. Currently, cordycepin hyperproduction of C. militaris was carried out in a liquid surface culture because of its low productivity in a submerged culture, however the reason was not known. In this study, 4.92 g/L of cordycepin was produced at the 15th day of C. militaris NBRC 103752 liquid surface culture, but only 1 mg/L was produced in the submerged culture. RNA-Seq was used to clarify the gene expression profiles of the cordycepin biosynthetic pathways of the submerged culture and the liquid surface culture...
2017: PloS One
https://www.readbyqxmd.com/read/29090553/-simultaneous-determination-of-cordycepin-and-2-deoxyadenosine-in-cordyceps-genus-by-online-spe-hplc
#20
Ye Tian, Chuan-Xi Wang, Zheng-Ming Qian, Zhou Li, Miao-Xia Zhou, Wan-Yang Sun, Xin-Sheng Yao, Wen-Jia Li, Hao Gao
An online SPE-HPLC method for simultaneous determination of cordycepin (3'-deoxyadenosine) and 2'-deoxyadenosine in Cordyceps genus (C. sinensis,C. militaris,Hirsutella sinensis and C. sobolifera) was developed. The samples were enriched on a ZORBAX SB-AQ (4.6 mm×12.5 mm,5 μm) column with isocratic elution by 9% methanol solution. The separation of analytes was performed on a ZORBAX SB-AQ (4.6 mm×150 mm,5 μm) column with gradient elution by 0.1% formic acid solution and methanol (91∶9). The flow rate was 1...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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