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Lian-Xian Guo, Yue-Hui Hong, Qian-Zhi Zhou, Qing Zhu, Xiao-Ming Xu, Jiang-Hai Wang
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March 19, 2018: Scientific Reports
Shih-Jeng Huang, Fu-Kuei Huang, Yu-Shan Li, Shu-Yao Tsai
Cordyceps militaris , a medicinal and edible mushroom, was used to ferment buckwheat and embryo rice by solid-state fermentation (SSF). Our aim was to investigate the effect of ultraviolet B (UVB) light irradiation on the content of vitamin D2 and biologically effective components, and antioxidant properties of buckwheat and embryo rice in SSF with C. militaris . Irradiated samples of buckwheat and embryo rice fermented by C. militaris had significantly increased vitamin D2 content, from 0-0.3 to 1.18-16.79 μg/g, while the increase in fresh embryo rice fermented by C...
December 2017: Food Technology and Biotechnology
Xiao-Fei Wu, Min Zhang, Bhesh Bhandari, Zhongqin Li
BACKGROUND: Microwave-assisted pulse-spouted bed freeze-drying (MPSFD) uses microwave heating to substitute the customary conduction heating during FD, resulting in high-quality dried samples with short drying time. The aim of this study was to determine the effect of MPSFD on volatile compounds and structural aspects of Cordyceps militaris. In order to highlight the effect of MPSFD, the freeze-drying (FD, recognized for its high-quality products) and hot air dying (HD, the most commonly used) were also evaluated using headspace solid phase micro-extraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and mid-Fourier transform infrared (FTIR) spectroscopy...
March 8, 2018: Journal of the Science of Food and Agriculture
Jian Jin, Wenli Kang, Can Zhong, You Qin, Rongrong Zhou, Hao Liu, Jing Xie, Lin Chen, Yuhui Qin, Shuihan Zhang
ETHNOPHARMACOLOGICAL RELEVANCE: The Yao ethnic group in Xuefeng Mountains area have used Xuefeng cordyceps, the caterpillar-fungus complex of Ophiocordyceps xuefengensis, for treating a variety of diseases for long. Just like some other cordyceps, O. xuefengensis, which is identified as the sister taxon of O. sinensis in 2013, also seems to have broad pharmacological properties, not only enhancing human immunity, anti-bacteria, anti-virus, but also anti-tumor. However, investigation of the medicinal fugal species O...
February 23, 2018: Journal of Ethnopharmacology
Ha-Kyoung Kwon, Woo-Ri Jo, Hye-Jin Park
BACKGROUND: Cordyceps militaris (C. militaris) is reported to exert various immune-activities. To enhance its activity, we fermented C.militaris with Pediococcus pentosaceus ON89A (GRC-ON89A). In this study, we investigated the immune-enhancing activity GRC-ON89A, using immunosuppressed model. METHODS: Immunosuppression was induced by intraperitoneal injection of cyclophosphamide (CY). Each group was orally administered distilled water, GRC-ON89A or GRC, respectively...
February 23, 2018: BMC Complementary and Alternative Medicine
Lei Yang, Xingai Jiao, Jinxiang Wu, Jiping Zhao, Tian Liu, Jianfeng Xu, Xiaohui Ma, Liuzao Cao, Lin Liu, Yahui Liu, Jingyu Chi, Minfang Zou, Shuo Li, Jiawei Xu, Liang Dong
Cordyceps sinensis is a traditional Chinese herbal medicine that has been used for centuries in Asia as a tonic to soothe the lung for the treatment of respiratory diseases. The aim of the present study was to determine the effects of C. sinensi s on airway remodeling in chronic obstructive pulmonary disease (COPD) and investigate the underlying molecular mechanisms. Rats with COPD were orally administered C. sinensis at low, moderate or high doses (2.5, 5 or 7.5 g/kg/day, respectively) for 12 weeks. Airway tissue histopathology, lung inflammation and airway remodeling were evaluated...
March 2018: Experimental and Therapeutic Medicine
Shuang Yang, Mingzhu Zheng, Yong Cao, Yanjiao Dong, Sanabil, Jingsheng Liu
Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130mL/500mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27...
February 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Tianqiao Yong, Shaodan Chen, Yizhen Xie, Diling Chen, Jiyan Su, Ou Shuai, Chunwei Jiao, Dan Zuo
Recently, we've reported the anti-hyperuricemic effects of Cordyceps militaris. As a characteristic compound of C. militaris, we hypothesized that cordycepin may play a role in preventing hyperurecimia. Remarkably, cordycepin produced important anti-hyperuricemic actions, decreasing SUA (serum uric acid) to 216, 210, and 203 μmol/L (P < 0.01) at 15, 30, and 60 mg/kg in comparison of hyperuricemic control (337 μmol/L), closing to normal control (202 μmol/L). Elisa, RT-PCR and western blot analysis demonstrated that the actions may be attributed to its downregulation of uric acid transporter 1 (URAT1) in kidney...
2018: Frontiers in Microbiology
P Chanjula, A Cherdthong
The objective of this experiment was to study the effects of dried spent mushroom Cordyceps militaris (SMCM) supplementation on digestibility, rumen fermentation, and blood metabolite parameters in goats. When the mushroom production and harvesting was complete, SMCM was collected fresh from a mushroom farm. Four 18-month-old male crossbred (Thai Native x Anglo Nubian) goats with initial body weight (BW) of 26.0 ±1.40 kg were randomly assigned according to a 4 × 4 Latin square design to receive four different levels of SMCM at 0, 100, 200, and 300 g/d...
February 2, 2018: Journal of Animal Science
Minghua Cong, Jiejun Wang, Yu Fang, Yinghua Liu, Mingxiao Sun, Qiong Wu, Kan Wang, Yan Huang, Yiqun Ling, Yong Liu, Quanfu Li, Yibing Liu, Jiang Zhu, Lingjun Zhu, Zhendong Zheng, Ling Li, Dongying Liu, Zimin Liu, Hanping Shi, Peng Yuan
OBJECTIVE: To investigate the current associated factors of dietary knowledge and behavior, the intake and nutritional status in malignancy Chinese inpatients, and the malnutrition causes involved in dietary nutrition knowledge level and behavior, providing recommendations to patients for nutrition education and intervention. METHOD: Five hundred and thirty-five participants from 18 hospitals were investigated by a questionnaire related to dietary knowledge and behavior...
February 5, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang Zhou, Xiaowei Li, Haiyan Li
BACKGROUND: Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the animal feed industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. RESULTS: We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide...
February 5, 2018: Microbial Cell Factories
Ke-Chun Bai, Fuu Sheu
Cordyceps militaris is a dietary therapeutic fungus that is an important model species in Cordyceps research. In this study, we purified a novel protein from the fruit bodies of C. militaris and designated it as Cordyceps militaris protein (CMP). CMP has a molecular mass of 18.0 kDa and is not glycosylated. Interestingly, CMP inhibited cell viability in murine primary cells and other cell lines in a time- and dose-dependent manner. Using trypan blue staining and a lactate dehydrogenase release assay, we showed that CMP caused cell death in the murine hepatoma cell line BNL 1MEA...
January 2018: Journal of Food and Drug Analysis
Liang-Tzung Lin, Ying-Jang Lai, She-Ching Wu, Wei-Hsuan Hsu, Chen-Jei Tai
Cordycepin is one of the most crucial bioactive compounds produced by Cordyceps militaris and has exhibited antitumor activity in various cancers. However, industrial production of large amounts of cordycepin is difficult. The porcine liver is abundant in proteins, vitamins, and adenosine, and these ingredients may increase cordycepin production and bioconversion during C. militaris fermentation. We observed that porcine liver extracts increased cordycepin production. In addition, air supply (2 h/d) significantly increased the cordycepin level in surface liquid-cultured C...
January 2018: Journal of Food and Drug Analysis
Bikash Baral
Members of the entomophagous fungi are considered very crucial in the fungal domain relative to their natural phenomenon and economic perspectives; however, inadequate knowledge of their mechanisms of interaction keeps them lagging behind in parallel studies of fungi associated with agro-ecology, forest pathology and medical biology. Ophiocordyceps sinensis (syn. Cordyceps sinensis), an intricate fungus-caterpillar complex after it parasitizes the larva of the moth, is a highly prized medicinal fungus known widely for ages due to its peculiar biochemical assets...
February 5, 2017: Journal of Fungi (Basel, Switzerland)
Gokhan Onder Erguven
In this study, we aimed to find out the biodegradation efficiency of some soil fungi types on herbicide acetochlor with acetochlor active material, chemical oxygen demand (COD), biochemical oxygen demand (BOD5) and total organic carbon (TOC) parameters. We also try to determine the population dynamics of these fungi via turbidity. The fungi cultures were isolated from agricultural field in in Thrace region of Turkey. Each fungi enriched in malt extract broth media and 1 mL of these enriched media was inoculated into 100 mL of acetochlor solutions that suggested for using application concantrations in agricultural field for farmers (11000 mg L-1) for sunflower and corn agriculture...
January 24, 2018: Bulletin of Environmental Contamination and Toxicology
Ken-Ichi Ojima, Arunrat Yangchum, Pattiyaa Laksanacharoen, Kanoksri Tasanathai, Donnaya Thanakitpipattana, Hidetoshi Tokuyama, Masahiko Isaka
Cordybislactone (3), a new stereoisomer of the 14-membered bislactone clonostachydiol, together with its open ring analog (4), was isolated from the hopper pathogenic fungus Cordyceps sp. BCC 49294. The relative and absolute configurations of 3 were determined by chemical derivatizations, including the modified Mosher's method. The stereochemistry of clonostachydiol was determined using the natural compound isolated from Xylaria sp. BCC 4297. The result revealed that the absolute configuration of clonostachydiol, previously determined by synthesis, should be revised to its enantiomer...
January 18, 2018: Journal of Antibiotics
Leandro Freire Dos Santos, Rosalia Rubel, Sandro Jose Ribeiro Bonatto, Adriana Aya Yamaguchi, Maria Fernanda Torres, Vanete Thomaz Soccol, Andre Luís Lopes da Silva, Carlos Ricardo Soccol
Macrophages have been considered an elusive yet emerging therapeutic target in tumor development since they are an important component in tumor microenvironment. The purpose of the present study was to evaluate the effect of C. sinensis on macrophage function (a component of tumor microenvironment which can alter the virulence of cancer) in high-fat diet fed rats. IMR-32 human neuroblastoma cell cytotoxicity was also investigated. The following parameters were observed to evaluate macrophage function: superoxide anion, hydrogen peroxide, nitric oxide, lysosomal volume and phagocytic capacity...
January 2018: Pakistan Journal of Pharmaceutical Sciences
Ang-Xin Song, Yu-Heng Mao, Ka-Chai Siu, Jian-Yong Wu
The bifidogenic effects of exopolysaccharide (EPS) of a medicinal fungus (Cordyceps sinensis) and a well-known food polysaccharide konjac glucomannan (KGM) with different molecular weight (MW) ranges were evaluated through in vitro experiments in liquid cultures of Bifidobacteria. Native EPS and KGM were partially degraded with power ultrasound (US) to improve the water solubility, and further hydrolysed with trifluoroacetic acid to much lower MW. The acid-hydrolysed fractions (EPS-AH and KGM-AH) supported the growth of all five tested bifidobacterial species, while the US-degraded high MW fractions, EPS-US and KGM-US, could only slightly supported the growth of some species...
January 12, 2018: International Journal of Biological Macromolecules
Yibo Huang, Yujiro Higuchi, Takashi Kinoshita, Ai Mitani, Yasunari Eshima, Kaoru Takegawa
Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl chloride (Dns)-labeled sialylglycan (Dns-SG) as a substrate, and discovered a strain that exhibits ENGase activity in the culture supernatant; this strain, named here as strain HMA12, was identified as a Sphingobacterium species by 16S ribosomal RNA gene analysis...
January 10, 2018: Scientific Reports
Julie-Anne Gandier, Emma R Master
The heterologous expression of proteins is often a crucial first step in not only investigating their function, but also in their industrial application. The functional assembly and aggregation of hydrophobins offers intriguing biotechnological applications from surface modification to drug delivery, yet make developing systems for their heterologous expression challenging. In this article, we describe the development of Pichia pastoris KM71H strains capable of solubly producing the full set of predicted Cordyceps militaris hydrophobins CMil1 (Class IA), CMil2 (Class II), and CMil3 (IM) at mg/L yields with the use of 6His-tags not only for purification but for their detection...
January 5, 2018: Microorganisms
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