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https://www.readbyqxmd.com/read/29137270/human-mesenchymal-stem-cells-ameliorate-experimental-pulmonary-hypertension-induced-by-maternal-inflammation-and-neonatal-hyperoxia-in-rats
#1
Chung-Ming Chen, Willie Lin, Liang-Ti Huang, Hsiu-Chu Chou
Pulmonary hypertension is a critical problem in infants with bronchopulmonary dysplasia. This study determined the therapeutic effects of human mesenchymal stem cells (MSCs) on pulmonary hypertension in an animal model. Pregnant Sprague-Dawley rats were intraperitoneally injected with lipopolysaccharide (LPS, 0.5 mg/kg/day) on gestational days 20 and 21. The pups were randomly assigned to two treatment conditions: room air (RA) or an O2-enriched atmosphere. On postnatal day 5, they were intratracheally transplanted with human MSCs (3 × 10(5) and 1 × 10(6) cells) in 0...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100396/combination-therapy-of-human-umbilical-cord-mesenchymal-stem-cells-and-fty720-attenuates-acute-lung-injury-induced-by-lipopolysaccharide-in-a-murine-model
#2
Zili Zhang, Wenfei Li, Zhizhi Heng, Jing Zheng, Puyuan Li, Xin Yuan, Wenkai Niu, Changqing Bai, Huiying Liu
ALI/ARDS remain the main reason of morbidity and mortality in the critically ill. Studies have indicated that human umbilical cord mesenchymal stem cells (hUC-MSCs) can be useful in the treatment of ALI/ARDS. Sphingosine-1-phosphate (S1P) and its analog FTY720 significantly reduce lipopolysaccharide (LPS)-induced lung edema and inflammatory lung injury. This study aimed to assess the therapeutic effects of hUC-MSCs combined with FTY720 in an LPS-induced murine model of ALI. Eight-week-old female C57BL/6 mice were divided into a normal control group, an LPS group, an hUC-MSC group, an FTY720 group, and an hUC-MSCs+FTY720 group randomly...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29096724/human-umbilical-cord-derived-mesenchymal-stem-cells-ameliorate-insulin-resistance-by-suppressing-nlrp3-inflammasome-mediated-inflammation-in-type-2-diabetes-rats
#3
Xiaoya Sun, Haojie Hao, Qingwang Han, Xiaoyan Song, Jiejie Liu, Liang Dong, Weidong Han, Yiming Mu
BACKGROUND: Insulin resistance is one of the most common and important pathological features of type 2 diabetes (T2D). Recently, insulin resistance is increasingly considered to be associated with systemic chronic inflammation. Elevated levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in blood are predictive indicators of the development of T2D. Mesenchymal stem cell (MSC)-based therapies have been proven to have potential immunomodulation and anti-inflammatory properties through their paracrine effects; however, the mechanism for the anti-inflammatory effect of MSCs in enhancing insulin sensitivity is still uncertain...
November 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29096716/galectin-1-and-galectin-3-expression-in-equine-mesenchymal-stromal-cells-mscs-synovial-fibroblasts-and-chondrocytes-and-the-effect-of-inflammation-on-msc-motility
#4
Heidi L Reesink, Ryan M Sutton, Carolyn R Shurer, Ryan P Peterson, Julie S Tan, Jin Su, Matthew J Paszek, Alan J Nixon
BACKGROUND: Mesenchymal stromal cells (MSCs) can be used intra-articularly to quell inflammation and promote cartilage healing; however, mechanisms by which MSCs mitigate joint disease remain poorly understood. Galectins, a family of β-galactoside binding proteins, regulate inflammation, adhesion and cell migration in diverse cell types. Galectin-1 and galectin-3 are proposed to be important intra-articular modulators of inflammation in both osteoarthritis and rheumatoid arthritis. Here, we asked whether equine bone marrow-derived MSCs (BMSCs) express higher levels of galectin-1 and -3 relative to synovial fibroblasts and chondrocytes and if an inflammatory environment affects BMSC galectin expression and motility...
November 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29072959/interleukin-10-overexpressing-mesenchymal-stromal-cells-induce-a-series-of-regulatory-effects-in-the-inflammatory-system-and-promote-the-survival-of-endotoxin-induced-acute-lung-injury-in-mice-model
#5
Chenfei Wang, Dan Lv, Xiaobin Zhang, Zhu-Ang Ni, Xiaofan Sun, Changqing Zhu
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are life-threatening inflammatory conditions with no effective pharmacological treatment. Previous studies suggested that mesenchymal stromal/stem cell (MSC) infusion resulted in better survival in mouse ALI models and presented low toxicity in human subjects. Therefore, in this study, we investigated the possibility of treating a murine model of ALI using MSCs with constant interleukin-10 overexpression (IL-10-MSC) by retroviral infection...
October 26, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28988712/antimicrobial-peptide-combined-with-bmp2-modified-mesenchymal-stem-cells-promotes-calvarial-repair-in-an-osteolytic-model
#6
Zunpeng Liu, Xue Yuan, Min Liu, Gabriela Fernandes, Yejia Zhang, Shuting Yang, Ciprian N Ionita, Shuying Yang
Repair and regeneration of inflammation-induced bone loss remains a clinical challenge. LL37, an antimicrobial peptide, plays critical roles in cell migration, cytokine production, apoptosis, and angiogenesis. Migration of stem cells to the affected site and promotion of vascularization are essential for tissue engineering therapy, including bone regeneration. However, it is largely unknown whether LL37 affects mesenchymal stem cell (MSC) behavior and bone morphogenetic protein 2 (BMP2)-mediated bone repair during the bone pathologic remodeling process...
September 14, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28969681/mesenchymal-stem-cells-microvesicles-stabilize-endothelial-barrier-function-partly-mediated-by-hepatocyte-growth-factor-hgf
#7
Hualing Wang, Ruiqiang Zheng, Qihong Chen, Jun Shao, Jiangquan Yu, Shuling Hu
BACKGROUND: Mesenchymal stem cells microvesicles (MSC-MVs) stabilize endothelial barrier function in acute lung injury (ALI); however, the detailed mechanism remains to be further defined. Hepatocyte growth factor (HGF), which is derived from MSC-MVs, might have a key role in the restoration of endothelial barrier function by MSC-MVs. METHODS: MSCs with lentiviral vector-mediated HGF gene knockdown (siHGF-MSC) were generated. A co-culture model of pulmonary microvascular endothelial cells and MSC-MVs collected from MSCs or siHGF-MSCs after 24 h of hypoxic culture was utilized...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28910300/acute-and-chronic-effects-of-treatment-with-mesenchymal-stromal-cells-on-lps-induced-pulmonary-inflammation-emphysema-and-atherosclerosis-development
#8
P Padmini S J Khedoe, Stan de Kleijn, Annemarie M van Oeveren-Rietdijk, Jaap J Plomp, Hetty C de Boer, Melissa van Pel, Patrick C N Rensen, Jimmy F P Berbée, Pieter S Hiemstra
BACKGROUND: COPD is a pulmonary disorder often accompanied by cardiovascular disease (CVD), and current treatment of this comorbidity is suboptimal. Systemic inflammation in COPD triggered by smoke and microbial exposure is suggested to link COPD and CVD. Mesenchymal stromal cells (MSC) possess anti-inflammatory capacities and MSC treatment is considered an attractive treatment option for various chronic inflammatory diseases. Therefore, we investigated the immunomodulatory properties of MSC in an acute and chronic model of lipopolysaccharide (LPS)-induced inflammation, emphysema and atherosclerosis development in APOE*3-Leiden (E3L) mice...
2017: PloS One
https://www.readbyqxmd.com/read/28843336/the-effect-of-trimetazidine-and-diazoxide-on-immunomodulatory-activity-of-human-embryonic-stem-cell-derived-mesenchymal-stem-cell-secretome
#9
Saeed Jahandideh, Faezeh Maghsood, Nastaran Mohammadi Ghahhari, Majid Lotfinia, Mohammad Mohammadi, Behrooz Johari, Mehdi Kadivar
Comprehensive proteome profiling of the factors secreted by mesenchymal stem cells (MSCs), referred to as secretome, revealed that it consists of cytokines, chemokines, growth factors, extracellular matrix proteins, and components of regeneration, vascularization, and hematopoiesis pathways. Harnessing this MSC secretome for therapeutic applications requires the optimization of production of secretary molecules. A variety of preconditioning methods have been introduced, which subject cells to stimulatory molecules to create the preferred response and stimulate persistent effects...
August 16, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28801676/hyaluronic-acid-coatings-as-a-simple-and-efficient-approach-to-improve-msc-homing-toward-the-site-of-inflammation
#10
Bruna Corradetti, Francesca Taraballi, Jonathan O Martinez, Silvia Minardi, Nupur Basu, Guillermo Bauza, Michael Evangelopoulos, Sebastian Powell, Claudia Corbo, Ennio Tasciotti
A major challenge in regenerative medicine is to improve therapeutic cells' delivery and targeting using an efficient and simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due to their powerful immunosoppressive potential. Here we report a simple and versatile method to transiently overexpress the hyaluronic acid (HA) receptor, CD44, on MSC membranes, to improve their homing potential towards an inflammatory site without affecting their behavior...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28768234/human-mesenchymal-stem-cells-ameliorate-experimental-pulmonary-hypertension-induced-by-maternal-inflammation-and-neonatal-hyperoxia-in-rats
#11
Chung-Ming Chen, Willie Lin, Liang-Ti Huang, Hsiu-Chu Chou
Pulmonary hypertension is a critical problem in infants with bronchopulmonary dysplasia. This study determined the therapeutic effects of human mesenchymal stem cells (MSCs) on pulmonary hypertension in an animal model. Pregnant Sprague-Dawley rats were intraperitoneally injected with lipopolysaccharide (LPS, 0.5 mg/kg/day) on gestational days 20 and 21. The pups were randomly assigned to two treatment conditions: room air (RA) or an O2-enriched atmosphere. On postnatal day 5, they were intratracheally transplanted with human MSCs (3 × 105 and 1 × 106 cells) in 0...
July 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28766061/lysophosphatidic-acid-enhances-human-umbilical-cord-mesenchymal-stem-cell-viability-without-differentiation-via-lpa-receptor-mediating-manner
#12
Narengerile Li, Ya-Li Yan, Sachaofu Fu, Rui-Juan Li, Peng-Fei Zhao, Xi-Yuan Xu, Jing-Ping Yang, Alatangaole Damirin
Human umbilical cord mesenchymal stem cells (hUC-MSCs) are potential stromal cells which are regarded as the most feasible stem cell group in cell therapy. The maintenance of cell survival without differentiation is important in cell transplantation and stem cell therapy. However, negative factors exist in cell transplantation. Lysophosphatidic acid (LPA) is a non-antigenic small molecule phospholipid which induced several fundamental cellular responses, such as cell proliferation, apoptosis and migration. In this study we aimed to explore the effects of LPA on the survival and differentiation of MSCs and its availability in cell therapy...
October 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28747098/effect-of-mesenchymal-stromal-cells-on-t-cells-in-a-septic-context-immunosuppression-or-immunostimulation
#13
Sébastien Le Burel, Cédric Thepenier, Laetitia Boutin, Jean-Jacques Lataillade, Juliette Peltzer
Sepsis is a complex process, including a first wave of damage partially due to the body's response to pathogens, followed by a phase of immune cell dysfunction. The efficacy of a pharmacological approach facing a rapidly evolving system implies a perfect timing of administration-this difficulty could explain the recent failure of clinical trials. Mesenchymal stromal cells (MSCs) are usually defined as immunosuppressive and their beneficial effects in preclinical models of acute sepsis have been shown to rely partly on such ability...
October 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28744636/nlr2-and-tlr3-tlr4-tlr5-ligands-injected-in-vivo-improve-after-1-h-the-efficiency-of-cloning-and-proliferative-activity-of-bone-marrow-multipotent-stromal-cells-and-reduce-the-content-of-osteogenic-multipotent-stromal-cells-in-cba-mice
#14
Yu F Gorskaya, A I Tukhvatulin, V G Nesterenko
Ligands NLR2 (muramyldipeptide) and TLR (bacterial LPS, flagellin, CpG-dinucleotide, and Poly I:C) and S. typhimurium antigenic complex by 1.5-3-fold increase the efficiency of cloning and content of multipotent stromal cells (MSC) in the bone marrow of CBA mice as soon as 1 h postinjection. The counts of large colonies (150-500 cells) increased by 2.5-3.3 times in comparison with intact bone marrow cultures at the expense of a decrease in the number of smaller colonies, which attests to enhanced proliferation of stromal cells in the colonies...
July 25, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28697979/the-effect-of-endothelial-cell-activation-and-hypoxia-on-placental-chorionic-mesenchymal-stem-stromal-cell-migration
#15
Balta Al-Sowayan, Rosemary J Keogh, Mohammed Abumaree, Harry M Georgiou, Bill Kalionis
INTRODUCTION: Chorionic mesenchymal stem/stromal cells (CMSC) can be isolated from the placenta in large numbers. Although their functions are yet to be fully elucidated, they have a role in tissue development and repair. To fulfil such a role, CMSC must be able to migrate to the microenvironment of the injury site. This process is not fully understood and the aim of this study therefore, was to examine in vitro CMSC migration in response to tissue inflammation and hypoxic conditioning...
June 27, 2017: Placenta
https://www.readbyqxmd.com/read/28667901/fibrous-scaffolds-potentiate-the-paracrine-function-of-mesenchymal-stem-cells-a-new-dimension-in-cell-material-interaction
#16
Ni Su, Peng-Lai Gao, Kai Wang, Jin-Yang Wang, Yi Zhong, Ying Luo
While the studies on the material interaction with mesenchymal stem cells (MSCs) have been mainly focused on the ability of materials to provide environment to regulate cell viability, proliferation or differentiation, the therapeutic effects of MSC-material constructs may result from the secretion of immunomodulatory and angiogenic cytokines from MSCs. Here, electrospun scaffolds composed of fibers in random, aligned and mesh-like patterns were fabricated, and the paracrine behavior of adipose-derived MSCs (Ad-MSCs) on the scaffolds were investigated in comparison to the cell culture via conventional microplates...
June 23, 2017: Biomaterials
https://www.readbyqxmd.com/read/28667247/astragalus-and-baicalein-regulate-inflammation-of-mesenchymal-stem-cells-mscs-by-the-mitogen-activated-protein-kinase-mapk-erk-pathway
#17
Lei Zhu, Ya-Jun Liu, Hong Shen, Pei-Qing Gu, Lu Zhang
BACKGROUND Mesenchymal stem cells (MSCs) have emerged as an attractive alternative to modulating immune response after transplantation. Recent studies have shown that systemically administered MSCs enter the inflamed intestine. In the present study, we propose a strategy to improve the efficacy of MSC-based cellular therapy for inflammation using Astragaloside and Baicalein to enhance cell survival, inhibit apoptosis, and modulate inflammatory response in vitro. MATERIAL AND METHODS MSCs were induced with lipopolysaccharide (LPS) as an inflammatory model before being treated for 48 h with Astragaloside, Baicalein, and the combination of both...
July 1, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28632668/low-intensity-pulsed-ultrasound-lipus-promotes-bmp9-induced-osteogenesis-and-suppresses-inflammatory-responses-in-human-periodontal-ligament-derived-stem-cells
#18
Joji Kusuyama, Toshiaki Nakamura, Tomokazu Ohnishi, Nahoko Eiraku, Kazuyuki Noguchi, Tetsuya Matsuguchi
OBJECTIVE: We previously reported that low intensity pulsed ultrasound (LIPUS) promotes marrow stromal cell (MSC) osteogenesis and suppresses the LPS-induced inflammatory response in osteoblasts. Here, we examined the effects of LIPUS on human periodontal ligament-derived stem cells (hPDLSCs) in chronic inflammatory bone disease, such as periodontitis. MATERIALS AND METHODS: hPDLSCs were collected from 3 healthy third molars. hPDLSCs were induced to differentiate by either recombinant BMP2 or BMP9 with or without daily LIPUS treatment (20 min/d)...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28598224/mesenchymal-stromal-cells-modulate-macrophages-in-clinically-relevant-lung-injury-models-by-extracellular-vesicle-mitochondrial-transfer
#19
Thomas J Morrison, Megan V Jackson, Erin K Cunningham, Adrien Kissenpfennig, Daniel F McAuley, Cecilia M O'Kane, Anna D Krasnodembskaya
RATIONALE: Acute Respiratory Distress Syndrome (ARDS) remains a major cause of respiratory failure in critically ill patients. Mesenchymal Stromal Cells (MSCs) are a promising candidate for a cell based therapy. However, the mechanisms of MSCs effects in ARDS are not well understood. Here we focused on the paracrine effect of MSCs on macrophage polarization and the role of extracellular vesicle (EV)-mediated mitochondrial transfer. OBJECTIVES: To determine the effects of human MSCs on macrophage function in the ARDS environment and to elucidate the mechanisms of these effects...
June 9, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28594941/low-level-laser-lll-attenuate-lps-induced-inflammatory-responses-in-mesenchymal-stem-cells-via-the-suppression-of-nf-%C3%AE%C2%BAb-signaling-pathway-in-vitro
#20
Kan Yin, Rongjia Zhu, Shihua Wang, Robert Chunhua Zhao
BACKGROUND: Considering promising results in animal models and patients, therapeutic use of MSCs for immune disease is likely to undergo continued evaluation. Low-lever laser (LLL) has been widely applied to retard the inflammatory reaction. LLL treatment can potentially be applied in anti-inflammatory therapy followed by stem cell therapy. AIM OF THE STUDY: The purpose of this study was to investigate the effect of LLL (660 nm) on the inflammatory reaction induced by LPS in human adipose derived mesenchymal stem cells (hADSCs) and pertinent mechanism...
2017: PloS One
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