keyword
MENU ▼
Read by QxMD icon Read
search

Tumor budding

keyword
https://www.readbyqxmd.com/read/28619527/expression-of-full-length-her2-protein-in-sf9-insect-cells-and-its-presentation-on-the-surface-of-budded-virus-like-particles
#1
Lisa Nika, Jakob Wallner, Dieter Palmberger, Krisztina Koczka, Karola Vorauer-Uhl, Reingard Grabherr
Biomarkers of cancer are often glycosylated membrane receptor proteins present on the cellular surface. In order to develop new antibodies for cancer diagnostics or treatment, it is a main pre-requisite that these target proteins are available in a native conformation. However, membrane receptor proteins are notoriously difficult to produce due to their hydrophobic nature and complex architecture. Here, we used the baculovirus-insect cell expression system to produce budded virus-like particles (VLPs) as the scaffold for the presentation of complex membrane proteins...
June 12, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28615209/in-vitro-replication-of-chelonid-herpesvirus-5-in-organotypic-skin-cultures-from-hawaiian-green-turtles-chelonia-mydas
#2
Thierry M Work, Julie Dagenais, Tina M Weatherby, George H Balazs, Mathias Ackermann
Fibropapillomatosis (FP) is a tumor disease of marine turtles associated with Chelonid herpesvirus 5 (ChHV5) that has historically been refractory to growth in tissue culture. Here, we show for the first time de novo formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative for active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included 1) either in-vitro culturing of ChHV5-positive tumor biopsies (plugs) or organotypic cultures (rafts) consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with turtle keratinocytes and 2) keratinocyte maturation induced by raising raft or biopsy cultures to the air-liquid interface...
June 14, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28558958/is-ep-cam-expression-a-diagnostic-and-prognostic-biomarker-for-colorectal-cancer-a-systematic-meta-analysis
#3
Susu Han, Shaoqi Zong, Qi Shi, Hongjia Li, Shanshan Liu, Wei Yang, Wen Li, Fenggang Hou
BACKGROUND: Cancer stem cell (CSC) epithelial cell adhesion molecule (Ep-CAM) is frequently expressed in colorectal cancer (CRC). However, the clinical significance of Ep-CAM expression in CRC is not clear. This study evaluated whether Ep-CAM provided valuable insight as a molecular biomarker for CRC diagnosis and prognosis and the potential of Ep-CAM as a novel therapeutic target in CRC. METHODS: Publications were selected online using electronic databases. The pooled odds ratios (ORs) or hazard ratios (HRs) with their 95% confidence intervals (95% CIs), and the combined sensitivity, specificity, and area under the curve (AUC) were calculated and summarized...
May 24, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28552992/microrna-200b-is-downregulated-in-colon-cancer-budding-cells
#4
Kirsten Nguyen Knudsen, Jan Lindebjerg, Boye Schnack Nielsen, Torben Frøstrup Hansen, Flemming Brandt Sørensen
BACKGROUND: The microRNA-200 (miR-200) family acts as a major suppressor of epithelial-mesenchymal transition (EMT). Impaired miR-200 expression may lead to EMT initiation and eventually cancer dissemination. The presence of tumor budding cells (TBC) is associated with metastasis and poor prognosis, and molecular similarities to EMT indicate that these cells may reflect ongoing EMT. The aim of this study was to investigate the expression of miR-200b in budding cells of colon cancer and the relationship with the EMT-markers E-cadherin, β-catenin and laminin-5γ2...
2017: PloS One
https://www.readbyqxmd.com/read/28548126/microrna-dysregulation-in-the-tumor-microenvironment-influences-the-phenotype-of-pancreatic-cancer
#5
Eva Karamitopoulou, Stefan Haemmig, Ulrich Baumgartner, Cornelia Schlup, Martin Wartenberg, Erik Vassella
Cellular interactions in the tumor microenvironment influence neoplastic progression in pancreatic ductal adenocarcinoma. One underlying mechanism is the induction of the prognostically unfavorable epithelial-mesenchymal-transition-like tumor budding. Our aim is to explore the expression of microRNAs implicated in the regulation of tumor budding focusing on the microenvironment of the invasive front. To this end, RNA from laser-capture-microdissected material of the main tumor, tumor buds, juxta-tumoral stroma, tumor-remote stroma, and non-neoplastic pancreatic parenchyma from pancreatic cancer cases with (n=7) and without (n=6) tumor budding was analyzed by qRT-PCR for the expression of a panel of miRNAs that are known to be implicated in the regulation of epithelial-mesenchymal transition, including miR-21, miR-183, miR-200b, miR-200c, miR-203, miR-205, miR-210, and miR-217...
May 26, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28548122/recommendations-for-reporting-tumor-budding-in-colorectal-cancer-based-on-the-international-tumor-budding-consensus-conference-itbcc-2016
#6
Alessandro Lugli, Richard Kirsch, Yoichi Ajioka, Fred Bosman, Gieri Cathomas, Heather Dawson, Hala El Zimaity, Jean-François Fléjou, Tine Plato Hansen, Arndt Hartmann, Sanjay Kakar, Cord Langner, Iris Nagtegaal, Giacomo Puppa, Robert Riddell, Ari Ristimäki, Kieran Sheahan, Thomas Smyrk, Kenichi Sugihara, Benoît Terris, Hideki Ueno, Michael Vieth, Inti Zlobec, Phil Quirke
Tumor budding is a well-established independent prognostic factor in colorectal cancer but a standardized method for its assessment has been lacking. The primary aim of the International Tumor Budding Consensus Conference (ITBCC) was to reach agreement on an international, evidence-based standardized scoring system for tumor budding in colorectal cancer. The ITBCC included nine sessions with presentations, a pre-meeting survey and an e-book covering the key publications on tumor budding in colorectal cancer...
May 26, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28505009/a-novel-grading-system-based-on-tumor-budding-and-cell-nest-size-is-a-strong-predictor-of-patient-outcome-in-esophageal-squamous-cell-carcinoma
#7
Moritz Jesinghaus, Melanie Boxberg, Björn Konukiewitz, Julia Slotta-Huspenina, Anna M Schlitter, Katja Steiger, Katja Specht, Kathrin Wieczorek, Arne Warth, Thomas Schmidt, Arndt Hartmann, Ihsan E Demir, Markus Feith, Katja Ott, Wilko Weichert
The determination of prognosis in patients with esophageal squamous cell carcinoma (ESCC) is primarily based on staging according to the TNM-classification, whereas conventional grading is of minor clinical importance because of its deficiencies in prognostic patient stratification. Recently, a novel, highly prognostic grading scheme based on budding activity and cell nest size has been proposed for squamous cell carcinoma (SCC) of both pulmonary as well as oral origin. In order to investigate the utility and transferability of this approach to ESCC, we evaluated budding activity and cell nest size, as well as other histomorphologic characteristics, in a cohort of 135 primarily resected tumors and correlated the results with clinicopathologic and outcome parameters...
May 12, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28498282/tumor-spread-through-air-spaces-is-an-independent-predictor-of-recurrence-free-survival-in-patients-with-resected-lung-squamous-cell-carcinoma
#8
Kyuichi Kadota, Yoshio Kushida, Naomi Katsuki, Ryou Ishikawa, Emi Ibuki, Mutsumi Motoyama, Kazuhito Nii, Hiroyasu Yokomise, Shuji Bandoh, Reiji Haba
Tumor spread through air spaces (STAS) is a newly recognized pattern of invasion in lung adenocarcinoma. However, clinical significance of STAS has not yet been characterized in lung squamous cell carcinoma. In this study, we investigated whether STAS could determine clinical outcome in Japanese patients with lung squamous cell carcinoma. We reviewed tumor slides from surgically resected lung squamous cell carcinomas (n=216). STAS was defined as tumor cells within air spaces in the lung parenchyma beyond the edge of the main tumor...
May 11, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28477021/transgenic-overexpression-of-nanogp8-in-the-mouse-prostate-is-insufficient-to-initiate-tumorigenesis-but-weakly-promotes-tumor-development-in-the-hi-myc-mouse-model
#9
Bigang Liu, Shuai Gong, Qiuhui Li, Xin Chen, John Moore, Mahipal V Suraneni, Mark D Badeaux, Collene R Jeter, Jianjun Shen, Rashid Mehmood, Qingxia Fan, Dean G Tang
This project was undertaken to address a critical cancer biology question: Is overexpression of the pluripotency molecule Nanog sufficient to initiate tumor development in a somatic tissue? Nanog1 is critical for the self-renewal and pluripotency of ES cells, and its retrotransposed homolog, NanogP8 is preferentially expressed in somatic cancer cells. Our work has shown that shRNA-mediated knockdown of NanogP8 in prostate, breast, and colon cancer cells inhibits tumor regeneration whereas inducible overexpression of NanogP8 promotes cancer stem cell phenotypes and properties...
April 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28475002/form-follows-function-morphological-and-immunohistological-insights-into-epithelial-mesenchymal-transition-characteristics-of-tumor-buds
#10
Kathrin Enderle-Ammour, Moritz Bader, Theresa Dorothee Ahrens, Kai Franke, Sylvia Timme, Agnes Csanadi, Jens Hoeppner, Birte Kulemann, Jochen Maurer, Philip Reiss, Oliver Schilling, Tobias Keck, Thomas Brabletz, Elmar Stickeler, Martin Werner, Ulrich Friedrich Wellner, Peter Bronsert
In cancer biology, the architectural concept "form follows function" is reflected by cell morphology, migration, and epithelial-mesenchymal transition protein pattern. In vivo, features of epithelial-mesenchymal transition have been associated with tumor budding, which correlates significantly with patient outcome. Hereby, the majority of tumor buds are not truly detached but still connected to a major tumor mass. For detailed insights into the different tumor bud types and the process of tumor budding, we quantified tumor cells according to histomorphological and immunohistological epithelial-mesenchymal transition characteristics...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28451002/-costal-metastasis-revealing-esophageal-squamous-cell-carcinoma
#11
Mamadou Ngoné Gueye, Gnagna Diouf, Daouda Dia, Awa Boye, Thierno Fall, Jean Louis Diémé, Oumar Ba, Mouhamadou Mbengue
Esophageal cancer is associated with poor prognosis. Its severity is linked to delayed diagnosis which is most often made once a cancer has metastasized, in Africa. Costal metastases are rare. We report a case of a 38-year old Senegalese patient with squamous cell carcinoma of the lower esophagus with lytic metastases to the ribs. Mrs. TD, aged 38, was admitted with painful swelling in right hemithorax associated with weight loss. The patient also reported mechanical dysphagia evolving during 4 months which had not motivated consultation...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28442513/histopathological-significance-and-prognostic-impact-of-tumor-budding-in-colorectal-cancer
#12
Anurag Mehta, Malini Goswami, Rupal Sinha
BACKGROUND: Colorectal cancer (CRC) is a heterogeneous disease with complex etiology. New prognostic factors for this group of disease need to be investigated. This study evaluated the histopathological significance and prognostic impact of tumor budding in CRC. DESIGN: A total of 60 treatment naive consecutive patients undergoing surgical resection for CRC during the period of January 2011 to December 2013 were included in the study. Details of each patient related to their demographic and tumor profile were recorded...
March 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28438617/tumor-budding-as-a-risk-factor-for-nodal-metastasis-in-pt1-colorectal-cancers-a-meta-analysis
#13
Rocco Cappellesso, Claudio Luchini, Nicola Veronese, Marcello Lo Mele, Erik Rosa-Rizzotto, Ennio Guido, Franca De Lazzari, Pierluigi Pilati, Fabio Farinati, Stefano Realdon, Marco Solmi, Matteo Fassan, Massimo Rugge
Worldwide, colorectal cancer (CRC) screening programs have significantly increased the detection of sub-mucosal (pT1) adenocarcinoma. Completion surgery may be indicated after endoscopic excision of these potentially metastasizing early cancers. However, the post-surgical prevalence of nodal implants does not exceed 15%, leading to questions concerning the clinical appropriateness of any post-endoscopy surgery. Eastern scientific societies (Japanese Society for Cancer of the Colon-Rectum, in particular) include tumor budding (TB), defined as the presence of isolated single cancer cells or clusters of fewer than five cancer cells at the tumor invasive front, among the variables that must be included in histological reports...
April 21, 2017: Human Pathology
https://www.readbyqxmd.com/read/28436947/dedifferentiation-into-blastomere-like-cancer-stem-cells-via-formation-of-polyploid-giant-cancer-cells
#14
N Niu, I Mercado-Uribe, J Liu
Our recent perplexing findings that polyploid giant cancer cells (PGCCs) acquired embryonic-like stemness and were capable of tumor initiation raised two important unanswered questions: how do PGCCs acquire such stemness, and to which stage of normal development do PGCCs correspond. Intriguingly, formation of giant cells due to failed mitosis/cytokinesis is common in the blastomere stage of the preimplantation embryo. However, the relationship between PGCCs and giant blastomeres has never been studied. Here, we tracked the fate of single PGCCs following paclitaxel-induced mitotic failure...
April 24, 2017: Oncogene
https://www.readbyqxmd.com/read/28428104/tumor-budding-in-intestinal-type-gastric-adenocarcinoma-is-associated-with-nodal-metastasis-and-recurrence
#15
Stephen Olsen, Jin Linda, Ryan C Fields, Yan Yan, ILKe Nalbantoglu
Gastric adenocarcinoma (GAC) is a common cause of cancer-related death worldwide. GAC can be classified as intestinal or diffuse. Intestinal type cancers are common and reported to have a better prognosis compared to diffuse cancers. Studies have shown the presence and amount of tumor budding in intestinal carcinomas of the colon and esophagus to predict nodal metastasis and recurrence. Our aim is to determine if tumor budding in intestinal type GAC correlates with prognostic features. One hundred four patients treated with primary surgical excision between 1999-2013 were identified...
April 17, 2017: Human Pathology
https://www.readbyqxmd.com/read/28416958/keratinization-of-lung-squamous-cell-carcinoma-is-associated-with-poor-clinical-outcome
#16
Hye Jung Park, Yoon-Jin Cha, Seong Han Kim, Arum Kim, Eun Young Kim, Yoon Soo Chang
BACKGROUND: Although the World Health Organization (WHO) classification of lung squamous cell carcinoma (SCC) was revised in 2015, its clinical implications for lung SCC subsets remain unclear. We investigated whether the morphologic characteristics of lung SCC, including keratinization, were associated with clinical parameters and clinical outcome of patients. METHODS: A total of 81 patients who underwent curative surgical resection of diagnosed lung SCC, were enrolled in this study...
April 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28415608/collagen-type-iv-alpha-1-col4a1-and-collagen-type-xiii-alpha-1-col13a1-produced-in-cancer-cells-promote-tumor-budding-at-the-invasion-front-in-human-urothelial-carcinoma-of-the-bladder
#17
Makito Miyake, Shunta Hori, Yosuke Morizawa, Yoshihiro Tatsumi, Michihiro Toritsuka, Sayuri Ohnishi, Keiji Shimada, Hideki Furuya, Vedbar S Khadka, Youping Deng, Kenta Ohnishi, Kota Iida, Daisuke Gotoh, Yasushi Nakai, Takeshi Inoue, Satoshi Anai, Kazumasa Torimoto, Katsuya Aoki, Nobumichi Tanaka, Noboru Konishi, Kiyohide Fujimoto
Current knowledge of the molecular mechanism driving tumor budding is limited. Here, we focused on elucidating the detailed mechanism underlying tumor budding in urothelial cancer of the bladder. Invasive urothelial cancer was pathologically classified into three groups as follows: nodular, trabecular, and infiltrative (tumor budding). Pathohistological analysis of the orthotopic tumor model revealed that human urothelial cancer cell lines MGH-U3, UM-UC-14, and UM-UC-3 displayed typical nodular, trabecular, and infiltrative patterns, respectively...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28407700/correction-dapk-loss-in-colon-cancer-tumor-buds-implications-for-migration-capacity-of-disseminating-tumor-cells
#18
Jelena Ivanovska, Inti Zlobec, Stefan Forster, Eva Karamitopoulou, Heather Dawson, Viktor Hendrik Koelzer, Abbas Agaimy, Fabian Garreis, Stephan Söder, William Laqua, Alessandro Lugli, Arndt Hartmann, Tilman T Rau, Regine Schneider-Stock
No abstract text is available yet for this article.
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28407462/overexpression-of-postn-in-tumor-stroma-is-a-poor-prognostic-indicator-of-colorectal-cancer
#19
Hyeon Jeong Oh, Jeong Mo Bae, Xian-Yu Wen, Nam-Yun Cho, Jung Ho Kim, Gyeong Hoon Kang
BACKGROUND: Tumor microenvironment has recently drawn attention in that it is related with tumor prognosis. Cancer-associated fibroblast also plays a critical role in cancer invasiveness and progression in colorectal cancers. Periostin (POSTN), originally identified to be expressed in osteoblasts and osteoblast-derived cells, is expressed in cancer-associated fibroblasts in several tissue types of cancer. Recent studies suggest an association between stromal overexpression of POSTN and poor prognosis of cancer patients...
May 2017: Journal of Pathology and Translational Medicine
https://www.readbyqxmd.com/read/28402788/ustilago-maydis-the-corn-smut-fungus-has-an-unusual-diploid-mitotic-stage
#20
Karen Snetselaar, Michael McCann
Ustilago maydis causes common smut disease in maize. Although pathogenic diploid strains of the fungus have been known for many years, the normal life cycle was thought to involve an extended dikaryotic stage, with nuclear fusion occurring in immature teliospores. However, microscopic examination of both living and fixed tumor material showed that nuclei fuse long before sporulation begins and that tumors are filled with uninucleate cells undergoing mitosis. Quantification of DNA in the nuclei confirmed these observations...
2017: Mycologia
keyword
keyword
69397
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"