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canine parvovirus

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https://www.readbyqxmd.com/read/28812526/parvovirus-infection-is-associated-with-myocarditis-and-myocardial-fibrosis-in-young-dogs
#1
Jordan Ford, Laura McEndaffer, Randall Renshaw, Alex Molesan, Kathleen Kelly
Perinatal parvoviral infection causes necrotizing myocarditis in puppies, which results in acute high mortality or progressive cardiac injury. While widespread vaccination has dramatically curtailed the epidemic of canine parvoviral myocarditis, we hypothesized that canine parvovirus 2 (CPV-2) myocardial infection is an underrecognized cause of myocarditis, cardiac damage, and/or repair by fibrosis in young dogs. In this retrospective study, DNA was extracted from formalin-fixed, paraffin-embedded tissues from 40 cases and 41 control dogs under 2 years of age from 2007 to 2015...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28810858/rapid-and-sensitive-detection-of-canine-distemper-virus-by-real-time-reverse-transcription-recombinase-polymerase-amplification
#2
Jianchang Wang, Jinfeng Wang, Ruiwen Li, Libing Liu, Wanzhe Yuan
BACKGROUND: Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Recombinase polymerase amplification (RPA), as an isothermal gene amplification technique, has been explored for the molecular detection of diverse pathogens. METHODS: A real-time reverse transcription RPA (RT-RPA) assay for the detection of canine distemper virus (CDV) using primers and exo probe targeting the CDV nucleocapsid protein gene was developed...
August 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28808849/a-rapid-method-for-establishment-of-a-reverse-genetics-system-for-canine-parvovirus
#3
Yongle Yu, Jun Su, Jigui Wang, Ji Xi, Yaping Mao, Qiang Hou, Xiaomei Zhang, Weiquan Liu
Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was indistinguishable from that of wild-type virus in its biological properties.
August 14, 2017: Virus Genes
https://www.readbyqxmd.com/read/28733230/clostridium-perfringens-and-c-%C3%A2-difficile-in-parvovirus-positive-dogs
#4
Rodrigo Otávio Silveira Silva, Fernanda Alves Dorella, Henrique Cesar Pereira Figueiredo, Érica Azevedo Costa, Vanessa Pelicia, Bruna Letícia Devidé Ribeiro, Marcio Garcia Ribeiro, Antonio Carlos Paes, Jane Megid, Francisco Carlos Faria Lobato
The aim of this study was to investigate Clostridium difficile and Clostridium perfringens in 82 diarrheic dogs positive for canine parvovirus type 2 (CPV). Enterotoxigenic C. perfringens type A was isolated from three (3.6%) dogs. One (1.2%) strain was also positive for NetE- and NetF-encoding genes, which are commonly associated with diarrhea in dogs. Toxigenic C. difficile was isolated from one animal (1.2%), which was also positive for A/B toxins. The present study identified C. difficile and C. perfringens infection in CPV-positive dogs...
July 19, 2017: Anaerobe
https://www.readbyqxmd.com/read/28716388/identification-of-co-infection-by-rotavirus-and-parvovirus-in-dogs-with-gastroenteritis-in-mexico
#5
Ariadna Flores Ortega, José Simón Martínez-Castañeda, Linda G Bautista-Gómez, Raúl Fajardo Muñoz, Israel Quijano Hernández
This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe.
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28716118/evolutionary-and-genetic-analysis-of-the-vp2-gene-of-canine-parvovirus
#6
Gairu Li, Senlin Ji, Xiaofeng Zhai, Yuxiang Zhang, Jie Liu, Mengyan Zhu, Jiyong Zhou, Shuo Su
BACKGROUND: Canine parvovirus (CPV) type 2 emerged in 1978 in the USA and quickly spread among dog populations all over the world with high morbidity. Although CPV is a DNA virus, its genomic substitution rate is similar to some RNA viruses. Therefore, it is important to trace the evolution of CPV to monitor the appearance of mutations that might affect vaccine effectiveness. RESULTS: Our analysis shows that the VP2 genes of CPV isolated from 1979 to 2016 are divided into six groups: GI, GII, GIII, GIV, GV, and GVI...
July 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28715293/surveillance-for-antibodies-against-six-canine-viruses-in-wild-raccoons-procyon-lotor-in-japan
#7
Emiko Aoki, Takehisa Soma, Mayumi Yokoyama, Makoto Matsubayashi, Kazumi Sasai
Raccoons (Procyon lotor) are found worldwide. They are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. We examined sera from 100 wild raccoons in Japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. We also aimed to understand the distribution of potentially infected wildlife. We found that 7% of samples were seropositive for canine distemper virus (CDV), 10% for canine parvovirus type (CPV), 2% for canine adenovirus type 1, 6% for canine adenovirus type 2, and 7% for canine coronavirus...
July 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28707272/first-molecular-characterization-of-canine-parvovirus-strains-in-sardinia-italy
#8
S Dei Giudici, T Cubeddu, A Giagu, G Sanna, S Rocca, A Oggiano
Canine parvovirus type 2 (CPV-2) is responsible of acute hemorrhagic gastroenteritis in young dogs. CPV-2 emerged in 1978 in the USA, but new antigenic types, CPV-2a, 2b and 2c, have completely replaced the original type. In this study, we analyzed 81 animals collected in Sardinia, Italy. The VP2 sequence analysis of 27 positive samples showed that all antigenic CPV-2 types are circulating. CPV-2b seems to be the most widespread variant, followed by CPV-2a. Furthermore, 12 CPV-2b strains displayed further amino acid substitutions and formed a separate cluster in a phylogenetic tree, indicating regional genetic variation...
July 14, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28689674/determination-of-immune-status-in-dogs-against-cpv-2-by-recombinant-protein-based-latex-agglutination-test
#9
Jobin Thomas, Mithilesh Singh, T K Goswami, Philma Glora, Soumendu Chakravarti, Vishal Chander, Vikramaditya Upmanyu, Suman Verma, Chhavi Sharma, K Mahendran
Canine parvoviral enteritis is a highly contagious viral illness caused by canine parvovirus-2 (CPV-2) which affects puppies of mainly 6-20 weeks of age. Vaccination is pivotal in preventing and controlling CPV-2 infection. Determination of antibody status is a critical determinant for successful vaccination. The hemagglutination inhibition (HI) test is 'gold standard' test for quantification of antibodies specific to CPV-2, although the execution of this test is not feasible under field conditions. The present study was undertaken to develop a point of care testing to determine immune status prior to CPV-2 vaccination or to detect seroconversion in immunized dogs by latex agglutination test (LAT) using recombinant antigen...
July 6, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28669382/new-wide-dynamic-range-assays-for-quantification-of-anti-leishmania-igg2-and-iga-antibodies-in-canine-serum
#10
Ana Cantos-Barreda, Damián Escribano, Luis J Bernal, Luis Pardo-Marín, José J Cerón, Silvia Martínez-Subiela
The aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-Leishmania IgG2 and IgA antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-Leishmania antibodies in Leishmania-seronegative and seropositive dogs, determined by a commercial ELISA assay, and between different clinical stages according to LeishVet guidelines. The analytical validation showed that the assays had a good precision with intra- and inter-assay coefficients of variation lower than 10%...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28657857/genetic-characterization-of-canine-parvovirus-in-sympatric-free-ranging-wild-carnivores-in-portugal
#11
Carla Miranda, Nuno Santos, Colin Parrish, Gertrude Thompson
Since its emergence in the 1970s, canine parvovirus (CPV) has been reported in domestic and nondomestic carnivores worldwide with severe implications on their health and survival. Here, we aim to better understand CPV circulation in multihost-pathogens systems by characterizing CPV DNA or viruses in 227 free-ranging wild carnivores of 12 species from Portugal. Collected samples during 1995-2011 were analyzed by PCR and sequence analysis. The canine parvovirus DNA was detected in 4 (2%) animals of two species, namely in wolves (Canis lupus; 3/63, 5%, 95% confidence interval=1...
June 28, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28639277/serum-derived-transfer-factor-stimulates-the-innate-immune-system-to-improve-survival-traits-in-high-risk-pathogen-scenarios
#12
Bridget V Willeford, Trudy Shapiro-Dunlap, Kenneth O Willeford
Preclinical Research Transfer Factors (TFs) are low molecular weight (<5,000 daltons) biological response mediators. In the present study, a serum derived TF improved the ability of the recipient animal to survive high-risk infectious challenges (salmonellosis and canine parvoviral enteritis (CPV)) by altering the host's cytokine response profile. Mice mortally challenged with 5,000 colony-forming units of Salmonella experienced a group mortality of 73% while mice treated with a single 5 mg dose of the TF demonstrated a significant decrease in morbidity (7%, p ≤ 0...
June 22, 2017: Drug Development Research
https://www.readbyqxmd.com/read/28635524/serology-and-protein-electrophoresis-for-evidence-of-exposure-to-12-mink-pathogens-in-free-ranging-american-mink-neovison-vison-in-argentina
#13
Pablo Eduardo Martino, Luis Ernesto Samartino, Néstor Oscar Stanchi, Nilda Esther Radman, Eduardo Joaquín Parrado
BACKGROUND: Basic pathologic characteristics for farmed minks were previously reported worldwide. However, its status in the wild has not been studied in detail. OBJECTIVE: Serology and electrophoresis were carried out for evidence of exposure to 12 mink pathogens on two different locations. ANIMALS AND METHODS: Serology was done in 87 wild minks by reference techniques against Toxoplasma gondii, Encephalitozoon cuniculi, Neospora caninum, Brucella abortus, Mycobacterium bovis, Leptospira interrogans, canine distemper virus (CDV), canine adenovirus (CAV), canine parvovirus (CPV), rabies virus (RV), Influenza A virus (FLUAV) and Aleutian disease virus (ADV)...
December 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28622497/feline-panleukopenia-virus-is-not-associated-with-myocarditis-or-endomyocardial-restrictive-cardiomyopathy-in-cats
#14
Laura McEndaffer, Alex Molesan, Hollis Erb, Kathleen Kelly
Canine parvovirus-2 (CPV-2) is nearly indistinguishable from feline panleukopenia virus (FPV) and is a well-known cause of viral myocarditis in young puppies; however, it is not known whether either FPV or CPV-2 naturally infects feline cardiomyocytes and causes myocarditis. Endomyocarditis (EMC) and left ventricular endomyocardial fibrosis (LVEF), clinically known as "endomyocardial restrictive cardiomyopathy," are important feline heart diseases suspected to have an infectious etiology. A continuum is suggested with EMC representing the acute reaction to an unknown infectious agent and LVEF the chronic manifestation of repair...
July 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28616485/evidence-of-canine-parvovirus-transmission-to-a-civet-cat-paradoxurus-musangus-in-singapore
#15
Ian H Mendenhall, Dolyce Low, Erica Sena Neves, Ali Anwar, Serena Oh, Yvonne C F Su, Gavin J D Smith
Cross-species transmission can often lead to deleterious effects in incidental hosts. Parvoviruses have a wide host range and primarily infect members of the order Carnivora. Here we describe juvenile common palm civet cats (Paradoxurus musangus) that were brought to the Singapore zoo and fell ill while quarantined. The tissues of two individual civets that died tested PCR-positive for parvovirus infection. Phylogenetic analysis revealed this parvovirus strain falls in a basal position to a clade of CPV that have infected dogs in China and Uruguay, suggesting cross-species transmission from domestic to wild animals...
December 2016: One Health
https://www.readbyqxmd.com/read/28603036/canine-parvovirus-type-2-cpv-2-and-feline-panleukopenia-virus-fpv-codon-bias-analysis-reveals-a-progressive-adaptation-to-the-new-niche-after-the-host-jump
#16
Giovanni Franzo, Claudia Maria Tucciarone, Mattia Cecchinato, Michele Drigo
Based on virus dependence from host cell machinery, their codon usage is expected to show a strong relation with the host one. Even if this association has been stated, especially for bacteria viruses, the linkage is considered to be less consistent for more complex organisms and a codon bias adaptation after host jump has never been proven. Canine parvovirus type 2 (CPV-2) was selected as a model because it represents a well characterized case of host jump, originating from Feline panleukopenia virus (FPV)...
June 8, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28569640/prevalence-of-serum-antibody-titers-against-canine-distemper-virus-and-canine-parvovirus-in-dogs-hospitalized-in-an-intensive-care-unit
#17
Jennifer L Mahon, Elizabeth A Rozanski, April L Paul
OBJECTIVE To determine the prevalence of dogs hospitalized in an intensive care unit (ICU) with serum antibody titers against canine distemper virus (CDV) and canine parvovirus (CPV). DESIGN Prospective observational study. ANIMALS 80 dogs. PROCEDURES Dogs hospitalized in an ICU for > 12 hours between February 1 and June 1, 2015, that had at least 0.25 mL of serum left over from diagnostic testing were eligible for study inclusion. Dogs with serum antibody titers > 1:32 (as determined by serum neutralization) and > 1:80 (as determined by hemagglutination inhibition) were considered seropositive for CDV and CPV, respectively...
June 15, 2017: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/28545817/pre-screening-of-crude-peptides-in-a-serological-bead-based-suspension-array
#18
Tinka Jelsma, Fimme J van der Wal, Helmi Fijten, Nicolas Dailly, Evert van Dijk, Willie L Loeffen
Most serological assays detect antibody responses in biological samples through affinity of serum antibodies for antigens provided in the assay. Certain antigens, however, may be difficult to produce and/or may contain unwanted epitopes. In these cases, a practical alternative may be the use of peptides as representatives for specific epitopes. Peptides can be obtained after purification in large quantities for a modest price, but screening of a large set of peptides during development may be relatively expensive...
May 22, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28532806/a-molecular-survey-for-selected-viral-enteropathogens-revealed-a-limited-role-of-canine-circovirus-in-the-development-of-canine-acute-gastroenteritis
#19
Giulia Dowgier, Eleonora Lorusso, Nicola Decaro, Costantina Desario, Viviana Mari, Maria Stella Lucente, Gianvito Lanave, Canio Buonavoglia, Gabriella Elia
Canine circovirus (CanineCV) is a canine virus, whose pathogenetic role is still uncertain. Based on recent data suggesting its role as entheropathogen, a case-control study was conducted between 2013 and 2016 to investigate the association of CanineCV with gastroenteritis in dogs, alone or in combination with other viral pathogens, including canine parvovirus (CPV), canine coronavirus (CCoV) and canine distemper virus (CDV). A total of 219 dogs suffering from acute gastroenteritis disorders and 67 controls randomly recruited among healthy dogs or patients presenting without enteric signs were screened by a panel of real-time (RT-)PCR assays for CanineCV, CPV, CCoV and CDV...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28523923/genetic-characterization-of-canine-parvovirus-from-dogs-in-pakistan
#20
M Z Shabbir, M U Sohail, U N Chaudhary, W Yaqub, I Rashid, M H Saleem, M Munir
Canine parvoviruses (CPV) exist as antigenic variants with varying frequencies and genetic variabilities across the globe. Given the endemicity and high prevalence in Pakistan, we characterized the CPVs originating from dogs-population to elucidate viral diversity and evolution. Fecal samples from clinically diseased pups (n = 17) of different breeds and age (2-6 months) were processed for hemagglutination assay (HA), and later for partial amplification of VP2 gene sequence and amino acid analysis. A total of 11 samples (64...
2017: Acta Virologica
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