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canine parvovirus

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https://www.readbyqxmd.com/read/27888408/extra-intestinal-detection-of-canine-kobuvirus-in-a-puppy-from-southern-brazil
#1
Juliane Ribeiro, Selwyn Arlington Headley, Jaqueline Assumpção Diniz, Alfredo Hajime Tanaka Pereira, Elis Lorenzetti, Amauri Alcindo Alfieri, Alice Fernandes Alfieri
This study presents the pathological, immunohistochemical, and molecular findings associated with the extra-intestinal detection of canine kobuvirus (CaKV) in a 5-month-old Chihuahua puppy, that had a clinical history of bloody-tinged feces. Principal pathological findings were interstitial pneumonia, necrotizing bronchitis, and parvovirus-induced enteritis. Molecular diagnostic methods identified CaKV within the cerebellum, cerebrum, lung, tonsil, and liver. CaKV and rotavirus were not identified within the feces and intestine...
November 25, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27888321/genetic-characterization-of-canine-parvovirus-type-2-subtypes-in-maputo-mozambique
#2
J Figueiredo, C Miranda, R Souto, E Silva, J Fafetine, G Thompson
Canine parvovirus type 2 (CPV-2) comprises three antigenic subtypes (2a, 2b and 2c) that have been reported in many countries. These subtypes cause serious disease in dogs with characteristic gastroenteritis signs. Little information has been documented in Africa about the genetic characterization of CPV-2. The aim of this study was to detect and to characterize the CPV-2 subtypes circulating in dogs admitted to Veterinary Clinics from two cities of Mozambique, Maputo and Matola, in 2010. A total of 40 field fecal samples were collected and tested for CPV-2 by polymerase chain reaction assay...
November 25, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27881651/alternative-polyadenylation-of-human-bocavirus-at-its-3-end-is-regulated-by-multiple-elements-and-affects-capsid-expression
#3
Sujuan Hao, Junmei Zhang, Zhen Chen, Huanzhou Xu, Hanzhong Wang, Wuxiang Guan
: Alternative processing of human bocavirus (HBoV) P5 promoter-transcribed RNA is critical for generating the structural and nonstructural protein-encoding mRNA transcripts. The regulatory mechanism by which HBoV RNA transcripts are polyadenylated at proximal [(pA)p] or distal [(pA)d] polyadenylation sites is yet unclear. We constructed a recombinant HBoV infectious clone to study the alternative polyadenylation regulation of HBoV. Surprisingly, in addition to the reported distal polyadenylation site (pA)d, a novel distal polyadenylation site (pA)d2 which is located in the right end hairpin (REH), was identified during infectious clone transfection or recombinant virus infection...
November 23, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27876613/multiplex-amplification-refractory-mutation-system-pcr-arms-pcr-provides-sequencing-independent-typing-of-canine-parvovirus
#4
Vishal Chander, Soumendu Chakravarti, Vikas Gupta, Sukdeb Nandi, Mithilesh Singh, Surendra Kumar Badasara, Chhavi Sharma, Mitesh Mittal, S Dandapat, V K Gupta
Canine parvovirus-2 antigenic variants (CPV-2a, CPV-2b and CPV-2c) ubiquitously distributed worldwide in canine population causes severe fatal gastroenteritis. Antigenic typing of CPV-2 remains a prime focus of research groups worldwide in understanding the disease epidemiology and virus evolution. The present study was thus envisioned to provide a simple sequencing independent, rapid, robust, specific, user-friendly technique for detecting and typing of presently circulating CPV-2 antigenic variants. ARMS-PCR strategy was employed using specific primers for CPV-2a, CPV-2b and CPV-2c to differentiate these antigenic types...
December 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27862411/natural-and-artificial-hyperimmune-solutions-impact-on-health-in-puppies
#5
H Mila, A Grellet, C Mariani, A Feugier, B Guard, J Suchodolski, J Steiner, S Chastant-Maillard
Colostrum and milk are complex mammary secretions providing the puppy with many nutritional and immunological factors, which play a crucial role for its correct development and survival. In the case of colostrum and/or milk intake deficiency, puppies are at increased risk of infectious diseases. This work reviews the various nutritional hyperimmune supplementations proposed to provide a passive immune protection and to positively impact puppies' health. Some strategies rely on canine immunoglobulins: canine colostrum banking and canine serum/plasma supplementation...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27847360/parvovirus-capsid-structures-required-for-infection-mutations-controlling-receptor-recognition-and-protease-cleavages
#6
Heather M Callaway, Kurtis H Feng, Donald W Lee, Andrew B Allison, Melissa Pinard, Robert McKenna, Mavis Agbandje-McKenna, Susan Hafenstein, Colin R Parrish
: Parvovirus capsids are small but complex molecular machines responsible for undertaking many of the steps of cell infection, genome packing, and cell-to-cell as well as host-to-host transfer. The details of parvovirus infection of cells are still not fully understood, but the processes must involve small changes in the capsid structure that allow the endocytosed virus to escape from the endosome, pass through the cell cytoplasm, and deliver the ssDNA genome to the nucleus where viral replication occurs...
November 9, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27818461/reliability-of-clinical-diagnosis-and-laboratory-testing-techniques-currently-used-for-identification-of-canine-parvovirus-enteritis-in-clinical-settings
#7
Mirna Faz, José Simón Martínez, Israel Quijano-Hernández, Raúl Fajardo
Canine parvovirus type 2 (CPV-2) is the main etiological agent of viral enteritis in dogs. Actually in literature, CPV-2 has been reported with clinical signs that vary from the classical disease, and immunochromatography test and PCR technique have been introduced to veterinary hospitals to confirm CPV-2 diagnosis and other infections. However, the reliability of these techniques has been poorly analyzed. In this study, we evaluated the sensitivity and specificity of veterinary clinical diagnosis, immunochromatography test and PCR technique...
November 6, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27771056/enteropathogen-infections-in-canine-puppies-co-occurrence-clinical-relevance-and-risk-factors
#8
Mirjam Duijvestijn, Lapo Mughini-Gras, Nancy Schuurman, Wim Schijf, Jaap A Wagenaar, Herman Egberink
Laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. The presence of potential pathogens however, does not prove a causal relationship with diarrhoea. The aim of this study was to identify specific enteropathogens in ≤12 month old puppies with and without acute diarrhoea and to assess their associations with clinical signs, putative risk factors and pathogen co-occurrence. Faecal samples from puppies with (n=113) and without (n=56) acute diarrhoea were collected and screened for Canine Parvovirus (CPV), Canine Coronavirus (CCoV), Salmonella spp...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27749192/the-occurrence-of-pathogens-in-an-endangered-population-of-american-badgers-taxidea-taxus-jacksoni-in-ontario-canada
#9
Danielle M Ethier, Joshua B Sayers, Christopher J Kyle, Joseph J Nocera, Davor Ojkic, Douglas Campbell
American badgers ( Taxidea taxus jacksoni) at the periphery of the species' range in Ontario, Canada, are listed as endangered because of an estimated population size of <200 mature individuals. The main threats faced by this population include habitat loss and road mortality. However, on 18 November 2013, a radio-implanted badger was found nonresponsive in an agricultural field with signs consistent with canine distemper virus infection, which was subsequently confirmed. This prompted our investigation into the occurrence of pathogens in this endangered carnivore to better quantify the level of risk infectious disease poses to population persistence...
October 17, 2016: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/27733797/first-detection-of-canine-parvovirus-type-2b-from-diarrheic-dogs-in-himachal-pradesh
#10
Shalini Sharma, Prasenjit Dhar, Aneesh Thakur, Vivek Sharma, Mandeep Sharma
AIM: The present study was conducted to detect the presence of canine parvovirus (CPV) among diarrheic dogs in Himachal Pradesh and to identify the most prevalent antigenic variant of CPV based on molecular typing and sequence analysis of VP2 gene. MATERIALS AND METHODS: A total of 102 fecal samples were collected from clinical cases of diarrhea or hemorrhagic gastroenteritis from CPV vaccinated or non-vaccinated dogs. Samples were tested using CPV-specific polymerase chain reaction (PCR) targeting VP2 gene, multiplex PCR for detection of CPV-2a and CPV-2b antigenic variants, and a PCR for the detection of CPV-2c...
September 2016: Veterinary World
https://www.readbyqxmd.com/read/27699205/seroprevalence-of-canine-parvovirus-in-dogs-in-lusaka-district-zambia
#11
Ngonda Saasa, King Shimumbo Nalubamba, Ethel M'kandawire, Joyce Siwila
Canine parvovirus (CPV) enteritis is a highly contagious enteric disease of young dogs. Limited studies have been done in Zambia to investigate the prevalence of CPV in dogs. Blood was collected from dogs from three veterinary clinics (clinic samples, n = 174) and one township of Lusaka (field samples, n = 56). Each dog's age, sex, breed, and vaccination status were recorded. A haemagglutination assay using pig erythrocytes and modified live parvovirus vaccine as the antigen was used. Antibodies to CPV were detected in 100% of dogs (unvaccinated or vaccinated)...
2016: Journal of veterinary medicine
https://www.readbyqxmd.com/read/27663840/identification-of-a-novel-canine-parvovirus-type-2c-in-taiwan
#12
Shu-Yun Chiang, Hung-Yi Wu, Ming-Tang Chiou, Min-Chen Chang, Chao-Nan Lin
BACKGROUND: Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiwan. In the present study, we characterized the previously unidentified CPV-2c variant and investigated the distribution of CPV-2 variants in Taiwan. METHODS: During January 2014 to April 2016, fecal or rectal swab samples from 99 dogs with suspected CPV-2 infection in Taiwan were collected...
2016: Virology Journal
https://www.readbyqxmd.com/read/27659245/direct-typing-of-canine-parvovirus-cpv-from-infected-dog-faeces-by-rapid-mini-sequencing-technique
#13
Pavana Jyothi V, Akila S, Malini K Selvan, Hariprasad Naidu, Shwethaa Raghunathan, Sathish Kota, R C Raja Sundaram, Samir Kumar Rana, G Dhinakar Raj, V A Srinivasan, B Mohana Subramanian
Canine parvovirus (CPV) is a non-enveloped single stranded DNA virus with an icosahedral capsid. Mini-sequencing based CPV typing was developed earlier to detect and differentiate all the CPV types and FPV in a single reaction. This technique was further evaluated in the present study by performing the mini-sequencing directly from fecal samples which avoided tedious virus isolation steps by cell culture system. Fecal swab samples were collected from 84 dogs with enteritis symptoms, suggestive of parvoviral infection from different locations across India...
September 19, 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/27590473/screening-red-foxes-vulpes-vulpes-for-possible-viral-causes-of-encephalitis
#14
Manon Bourg, Daniel Nobach, Sibylle Herzog, Hildburg Lange-Herbst, Anne Nesseler, Hans-Peter Hamann, Sabrina Becker, Dirk Höper, Bernd Hoffmann, Markus Eickmann, Christiane Herden
BACKGROUND: Next to various known infectious and non-infectious causes, the aetiology of non-suppurative encephalitis in red foxes (Vulpes vulpes) often remains unclear. Known causes in foxes imply rabies, canine distemper, toxoplasmosis, Aujeszky's disease, as well as parvovirus, adenovirus, circovirus and flavivirus infections. In this study, particular attention was paid on bornaviruses, since red foxes are predators of bicoloured white-toothed shrews, a reservoir of Borna disease virus 1 (BoDV-1)...
2016: Virology Journal
https://www.readbyqxmd.com/read/27535057/near-atomic-resolution-structure-of-a-highly-neutralizing-fab-bound-to-canine-parvovirus
#15
Lindsey J Organtini, Hyunwook Lee, Sho Iketani, Kai Huang, Robert E Ashley, Alexander M Makhov, James F Conway, Colin R Parrish, Susan Hafenstein
: Canine parvovirus (CPV) is a highly contagious pathogen that causes severe disease in dogs and wildlife. Previously, a panel of neutralizing monoclonal antibodies (MAb) raised against CPV was characterized. An antibody fragment (Fab) of MAb E was found to neutralize the virus at low molar ratios. Using recent advances in cryo-electron microscopy (cryo-EM), we determined the structure of CPV in complex with Fab E to 4.1 Å resolution, which allowed de novo building of the Fab structure...
November 1, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27502089/molecular-characterisation-of-parvoviruses-from-domestic-cats-reveals-emergence-of-newer-variants-in-india
#16
Hirak K Mukhopadhyay, Mangadevi Nookala, Nobal Rk Thangamani, Amsaveni Sivaprakasam, Prabhakar X Antony, Jacob Thanislass, Mouttou V Srinivas, Raghavan M Pillai
OBJECTIVES: The present study was undertaken to characterise the viral polypeptide 2 (VP2) gene of parvovirus from domestic cats in India. METHODS: The faecal samples from diarrhoeic/healthy domestic cats were collected from different geographical regions of India for screening by PCR assay followed by sequence analysis of the VP2 gene. RESULTS: Canine parvovirus (CPV)/feline panleukopenia virus (FPV) infections were found in 12 samples (11...
August 8, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27469023/exposure-of-free-ranging-wild-carnivores-and-domestic-dogs-to-canine-distemper-virus-and-parvovirus-in-the-cerrado-of-central-brazil
#17
Mariana Malzoni Furtado, Erika Midori Kida Hayashi, Susan Dora Allendorf, Claudio José Coelho, Anah Tereza de Almeida Jácomo, Jane Megid, José Domingues Ramos Filho, Leandro Silveira, Natália Mundim Tôrres, José Soares Ferreira Neto
Human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. This study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (CDV) and parvovirus in Emas National Park (ENP) in the Cerrado savanna of central Brazil. Serum samples were collected from 169 wild carnivores, including the maned wolf (Chrysocyon brachyurus), crab-eating fox (Cerdocyon thous), hoary fox (Pseudalopex vetulus), puma (Puma concolor), ocelot (Leopardus pardalis), pampas cat (Leopardus colocolo), jaguarundi (Herpailurus yagouaroundi), striped hog-nosed skunk (Conepatus semistriatus) and coati (Nasua nasua), and from 35 domestic dogs living on rural properties bordering ENP...
September 2016: EcoHealth
https://www.readbyqxmd.com/read/27461242/hepatitis-e-virus-derived-from-different-sources-exhibits-different-behaviour-in-virus-inactivation-and-or-removal-studies-with-plasma-derivatives
#18
Mikihiro Yunoki, Hiroyuki Tanaka, Kadue Takahashi, Takeru Urayama, Shinji Hattori, Shoji Ideno, Rie Furuki, Kaoru Sakai, Katsuro Hagiwara, Kazuyoshi Ikuta
Hepatitis E virus (HEV) causes viral hepatitis, and is considered a risk factor for blood products. Although some HEV inactivation/removal studies have been reported, detailed investigations of different manufacturing steps as heat treatment, partitioning during cold ethanol fractionation, low pH treatment, and virus filtration have yet to be reported for plasma-derived medicinal products. In this study, human serum- and swine faeces-derived HEVs, with and without detergent treatment, were used. The kinetic patterns of inactivation, log reduction value, or partitioning during the process were evaluated...
September 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27449079/two-potential-recombinant-rabies-vaccines-expressing-canine-parvovirus-virion-protein-2-induce-immunogenicity-to-canine-parvovirus-and-rabies-virus
#19
Jun Luo, Hehe Shi, Yeping Tan, Xuefeng Niu, Teng Long, Jing Zhao, Qin Tian, Yifei Wang, Hao Chen, Xiaofeng Guo
Both rabies virus (RABV) and canine parvovirus (CPV) cause lethal diseases in dogs. In this study, both high egg passage Flury (HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein (G) gene were used to express the CPV virion protein 2 (VP2) gene, and were designated rHEP-VP2 and, rHEP-dG-VP2 respectively. The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury. Western blotting indicated that G protein and VP2 were expressed in vitro...
August 17, 2016: Vaccine
https://www.readbyqxmd.com/read/27406444/profiling-of-host-cell-response-to-successive-canine-parvovirus-infection-based-on-kinetic-proteomic-change-identification
#20
Hang Zhao, Yuening Cheng, Jianke Wang, Peng Lin, Li Yi, Yaru Sun, Jingqiang Ren, Mingwei Tong, Zhigang Cao, Jiawei Li, Jinliang Deng, Shipeng Cheng
Canine parvovirus (CPV) reproduces by co-opting the resources of host cells, inevitably causing cytotoxic effects to the host cells. Feline kidney F81 cells are sensitive to CPV infection and show disparate growing statuses at different time points post-infection. This study analysed the response of F81 cells to CPV infection at successive infection time points by iTRAQ-based quantitative proteomics. Differentially expressed proteins (DEPs) during 60 h of infection and at selected time points post-infection were identified by an analysis of variance test and a two-tailed unpaired t test, respectively...
2016: Scientific Reports
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