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https://www.readbyqxmd.com/read/27918639/evaluation-of-an-outpatient-protocol-in-the-treatment-of-canine-parvoviral-enteritis
#1
Emilee C Venn, Karolina Preisner, Pedro L Boscan, David C Twedt, Lauren A Sullivan
OBJECTIVE: To compare 2 treatment protocols (standard in-hospital versus modified outpatient) in affecting the duration of treatment or survival of dogs with parvoviral enteritis. DESIGN: Prospective, randomized study. SETTING: University teaching hospital. ANIMALS: Client-owned dogs with naturally acquired parvovirus were randomized to receive either an inpatient (n = 20) or outpatient (n = 20) treatment protocol. INTERVENTIONS: Both groups received intravenous (IV) fluid resuscitation and correction of hypoglycemia at hospital admission...
December 5, 2016: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/27888408/extra-intestinal-detection-of-canine-kobuvirus-in-a-puppy-from-southern-brazil
#2
Juliane Ribeiro, Selwyn Arlington Headley, Jaqueline Assumpção Diniz, Alfredo Hajime Tanaka Pereira, Elis Lorenzetti, Amauri Alcindo Alfieri, Alice Fernandes Alfieri
This study presents the pathological, immunohistochemical, and molecular findings associated with the extra-intestinal detection of canine kobuvirus (CaKV) in a 5-month-old Chihuahua puppy, that had a clinical history of bloody-tinged feces. Principal pathological findings were interstitial pneumonia, necrotizing bronchitis, and parvovirus-induced enteritis. Molecular diagnostic methods identified CaKV within the cerebellum, cerebrum, lung, tonsil, and liver. CaKV and rotavirus were not identified within the feces and intestine...
November 25, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27888321/genetic-characterization-of-canine-parvovirus-type-2-subtypes-in-maputo-mozambique
#3
J Figueiredo, C Miranda, R Souto, E Silva, J Fafetine, G Thompson
Canine parvovirus type 2 (CPV-2) comprises three antigenic subtypes (2a, 2b and 2c) that have been reported in many countries. These subtypes cause serious disease in dogs with characteristic gastroenteritis signs. Little information has been documented in Africa about the genetic characterization of CPV-2. The aim of this study was to detect and to characterize the CPV-2 subtypes circulating in dogs admitted to Veterinary Clinics from two cities of Mozambique, Maputo and Matola, in 2010. A total of 40 field fecal samples were collected and tested for CPV-2 by polymerase chain reaction assay...
November 25, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27818461/reliability-of-clinical-diagnosis-and-laboratory-testing-techniques-currently-used-for-identification-of-canine-parvovirus-enteritis-in-clinical-settings
#4
Mirna Faz, José Simón Martínez, Israel Quijano-Hernández, Raúl Fajardo
Canine parvovirus type 2 (CPV-2) is the main etiological agent of viral enteritis in dogs. Actually in literature, CPV-2 has been reported with clinical signs that vary from the classical disease, and immunochromatography test and PCR technique have been introduced to veterinary hospitals to confirm CPV-2 diagnosis and other infections. However, the reliability of these techniques has been poorly analyzed. In this study, we evaluated the sensitivity and specificity of veterinary clinical diagnosis, immunochromatography test and PCR technique...
November 6, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27771056/enteropathogen-infections-in-canine-puppies-co-occurrence-clinical-relevance-and-risk-factors
#5
Mirjam Duijvestijn, Lapo Mughini-Gras, Nancy Schuurman, Wim Schijf, Jaap A Wagenaar, Herman Egberink
Laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. The presence of potential pathogens however, does not prove a causal relationship with diarrhoea. The aim of this study was to identify specific enteropathogens in ≤12 month old puppies with and without acute diarrhoea and to assess their associations with clinical signs, putative risk factors and pathogen co-occurrence. Faecal samples from puppies with (n=113) and without (n=56) acute diarrhoea were collected and screened for Canine Parvovirus (CPV), Canine Coronavirus (CCoV), Salmonella spp...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27733797/first-detection-of-canine-parvovirus-type-2b-from-diarrheic-dogs-in-himachal-pradesh
#6
Shalini Sharma, Prasenjit Dhar, Aneesh Thakur, Vivek Sharma, Mandeep Sharma
AIM: The present study was conducted to detect the presence of canine parvovirus (CPV) among diarrheic dogs in Himachal Pradesh and to identify the most prevalent antigenic variant of CPV based on molecular typing and sequence analysis of VP2 gene. MATERIALS AND METHODS: A total of 102 fecal samples were collected from clinical cases of diarrhea or hemorrhagic gastroenteritis from CPV vaccinated or non-vaccinated dogs. Samples were tested using CPV-specific polymerase chain reaction (PCR) targeting VP2 gene, multiplex PCR for detection of CPV-2a and CPV-2b antigenic variants, and a PCR for the detection of CPV-2c...
September 2016: Veterinary World
https://www.readbyqxmd.com/read/27699205/seroprevalence-of-canine-parvovirus-in-dogs-in-lusaka-district-zambia
#7
Ngonda Saasa, King Shimumbo Nalubamba, Ethel M'kandawire, Joyce Siwila
Canine parvovirus (CPV) enteritis is a highly contagious enteric disease of young dogs. Limited studies have been done in Zambia to investigate the prevalence of CPV in dogs. Blood was collected from dogs from three veterinary clinics (clinic samples, n = 174) and one township of Lusaka (field samples, n = 56). Each dog's age, sex, breed, and vaccination status were recorded. A haemagglutination assay using pig erythrocytes and modified live parvovirus vaccine as the antigen was used. Antibodies to CPV were detected in 100% of dogs (unvaccinated or vaccinated)...
2016: Journal of veterinary medicine
https://www.readbyqxmd.com/read/27663840/identification-of-a-novel-canine-parvovirus-type-2c-in-taiwan
#8
Shu-Yun Chiang, Hung-Yi Wu, Ming-Tang Chiou, Min-Chen Chang, Chao-Nan Lin
BACKGROUND: Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiwan. In the present study, we characterized the previously unidentified CPV-2c variant and investigated the distribution of CPV-2 variants in Taiwan. METHODS: During January 2014 to April 2016, fecal or rectal swab samples from 99 dogs with suspected CPV-2 infection in Taiwan were collected...
2016: Virology Journal
https://www.readbyqxmd.com/read/27659245/direct-typing-of-canine-parvovirus-cpv-from-infected-dog-faeces-by-rapid-mini-sequencing-technique
#9
Pavana Jyothi V, Akila S, Malini K Selvan, Hariprasad Naidu, Shwethaa Raghunathan, Sathish Kota, R C Raja Sundaram, Samir Kumar Rana, G Dhinakar Raj, V A Srinivasan, B Mohana Subramanian
Canine parvovirus (CPV) is a non-enveloped single stranded DNA virus with an icosahedral capsid. Mini-sequencing based CPV typing was developed earlier to detect and differentiate all the CPV types and FPV in a single reaction. This technique was further evaluated in the present study by performing the mini-sequencing directly from fecal samples which avoided tedious virus isolation steps by cell culture system. Fecal swab samples were collected from 84 dogs with enteritis symptoms, suggestive of parvoviral infection from different locations across India...
September 19, 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/27535057/near-atomic-resolution-structure-of-a-highly-neutralizing-fab-bound-to-canine-parvovirus
#10
Lindsey J Organtini, Hyunwook Lee, Sho Iketani, Kai Huang, Robert E Ashley, Alexander M Makhov, James F Conway, Colin R Parrish, Susan Hafenstein
: Canine parvovirus (CPV) is a highly contagious pathogen that causes severe disease in dogs and wildlife. Previously, a panel of neutralizing monoclonal antibodies (MAb) raised against CPV was characterized. An antibody fragment (Fab) of MAb E was found to neutralize the virus at low molar ratios. Using recent advances in cryo-electron microscopy (cryo-EM), we determined the structure of CPV in complex with Fab E to 4.1 Å resolution, which allowed de novo building of the Fab structure...
November 1, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27469023/exposure-of-free-ranging-wild-carnivores-and-domestic-dogs-to-canine-distemper-virus-and-parvovirus-in-the-cerrado-of-central-brazil
#11
Mariana Malzoni Furtado, Erika Midori Kida Hayashi, Susan Dora Allendorf, Claudio José Coelho, Anah Tereza de Almeida Jácomo, Jane Megid, José Domingues Ramos Filho, Leandro Silveira, Natália Mundim Tôrres, José Soares Ferreira Neto
Human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. This study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (CDV) and parvovirus in Emas National Park (ENP) in the Cerrado savanna of central Brazil. Serum samples were collected from 169 wild carnivores, including the maned wolf (Chrysocyon brachyurus), crab-eating fox (Cerdocyon thous), hoary fox (Pseudalopex vetulus), puma (Puma concolor), ocelot (Leopardus pardalis), pampas cat (Leopardus colocolo), jaguarundi (Herpailurus yagouaroundi), striped hog-nosed skunk (Conepatus semistriatus) and coati (Nasua nasua), and from 35 domestic dogs living on rural properties bordering ENP...
September 2016: EcoHealth
https://www.readbyqxmd.com/read/27449079/two-potential-recombinant-rabies-vaccines-expressing-canine-parvovirus-virion-protein-2-induce-immunogenicity-to-canine-parvovirus-and-rabies-virus
#12
Jun Luo, Hehe Shi, Yeping Tan, Xuefeng Niu, Teng Long, Jing Zhao, Qin Tian, Yifei Wang, Hao Chen, Xiaofeng Guo
Both rabies virus (RABV) and canine parvovirus (CPV) cause lethal diseases in dogs. In this study, both high egg passage Flury (HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein (G) gene were used to express the CPV virion protein 2 (VP2) gene, and were designated rHEP-VP2 and, rHEP-dG-VP2 respectively. The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury. Western blotting indicated that G protein and VP2 were expressed in vitro...
August 17, 2016: Vaccine
https://www.readbyqxmd.com/read/27389721/canine-parvovirus-the-worldwide-occurrence-of-antigenic-variants
#13
Carla Miranda, Gertrude Thompson
The most important enteric virus infecting canids is canine parvovirus type 2 (CPV-2). CPV is the aetiologic agent of a contagious disease, mainly characterized by clinical gastroenteritis signs in younger dogs. CPV-2 emerged as a new virus in the late 1970s, which could infect domestic dogs, and became distributed in the global dog population within 2 years. A few years later, the virus's original type was replaced by a new genetic and antigenic variant, called CPV-2a. Around 1984 and 2000, virus variants with the single change to Asp or Glu in the VP2 residue 426 were detected (sometimes termed CPV-2b and -2c)...
September 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27380652/feline-panleukopaenia-virus-in-captive-non-domestic-felids-in-south-africa
#14
Emily P Lane, Helene Brettschneider, Peter Caldwell, Almero Oosthuizen, Desiré L Dalton, Liza du Plessis, Johan Steyl, Antoinette Kotze
An outbreak of feline panleukopaenia virus (FPLV) infection was diagnosed by pathology, electron microscopy and polymerase chain reaction (PCR) in vaccinated captive-bred subadult cheetahs in South Africa. Subsequent to this disease outbreak, 12 cases of FPLV diagnosed on histology were confirmed by PCR in captive African black-footed cat, caracal, cheetah, lion, ocelot and serval. Phylogenetic analyses of the viral capsid protein gene on PCR-positive samples, vaccine and National Center for Biotechnology Information (NCBI) reference strains identified a previously unknown strain of FPLV, present since at least 2006, that differs from both the inactivated and the modified live vaccine strains...
2016: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/27377264/occurrence-of-canine-parvovirus-in-dogs-from-henan-province-of-china-in-2009-2014
#15
Zhanqin Zhao, Huisheng Liu, Ke Ding, Chunping Peng, Qiao Xue, Zuhua Yu, Yun Xue
BACKGROUND: There is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China. Therefore, the aim of the present study was to provide insights into the epidemiology and molecular characterization of CPV circulating in Henan province from 2009 to 2014. RESULTS: Nineteen thousand nine hundred seven dogs from pet hospitals in the cities of Luoyang, Anyang, Jiaozuo, Sanmenxia, Xinxiang, Zhengzhou in Henan province between 2009 and 2014 were investigated...
2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27354304/identification-of-a-novel-aleutian-mink-disease-virus-b-cell-epitope-using-a-monoclonal-antibody-against-vp2-protein
#16
Li Yi, Yuening Cheng, Miao Zhang, Zhigang Cao, Mingwei Tong, Shipeng Cheng, Xijun Yan
Aleutian mink disease virus (AMDV) is a parvovirus that causes an immune complex-mediated disease in minks. Capsid protein VP2 is a major structural viral protein and can be used to diagnose AMDV. In this study, a specific monoclonal antibody, 1M13, was produced against the AMDV VP2 protein (amino acids 291-502). A linear VP2-protein epitope was identified by subjecting a series of partially overlapping synthesized peptides to be enzyme-linked immunosorbent assay (ELISA) analysis. The results indicated that (386)HLQQNFSTRYIYD(398) was the minimal linear epitope that could be recognized by mAb 1M13...
September 2, 2016: Virus Research
https://www.readbyqxmd.com/read/27339228/transcriptome-profiling-indicating-canine-parvovirus-type-2a-as-a-potential-immune-activator
#17
Xu-Xu Fan, Yuan Gao, Long Shu, Yan-Quan Wei, Xue-Ping Yao, Sui-Zhong Cao, Guang-Neng Peng, Xiang-Tao Liu, Shi-Qi Sun
Canine parvovirus type 2a (CPV-2a) is a variant of CPV-2, which is a highly contagious pathogen causing severe gastroenteritis and death in young dogs. However, how CPV-2 participates in cell regulation and immune response remains unknown. In this study, persistently infected MDCK cells were generated through culture passage of the CPV-2a-infected cells for ten generations. Our study showed that CPV-2a induces cell proliferation arrest and cell morphology alternation before the fourth generation, whereas, the cell morphology returns to normal after five times of passages...
June 23, 2016: Virus Genes
https://www.readbyqxmd.com/read/27279352/influence-of-breed-size-age-fecal-quality-and-enteropathogen-shedding-on-fecal-calprotectin-and-immunoglobulin-a-concentrations-in-puppies-during-the-weaning-period
#18
A Grellet, R M Heilmann, B Polack, A Feugier, C Boucraut-Baralon, D Grandjean, N Grützner, J S Suchodolski, J M Steiner, S Chastant-Maillard
BACKGROUND: Fecal calprotectin and immunoglobulin A (IgA) are markers of intestinal inflammation and immunity in adult dogs. HYPOTHESIS: Fecal calprotectin and IgA concentrations in puppies are not influenced by fecal moisture in puppies but by enteropathogen shedding. ANIMALS: Three hundred and twenty-four puppies. METHODS: Fecal consistency was assessed by gross examination. Fecal moisture was evaluated before and after lyophilization...
July 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27195685/prevalence-of-antibodies-to-selected-viruses-and-parasites-in-introduced-and-endemic-carnivores-in-western-madagascar
#19
Julie Pomerantz, Fidisoa T Rasambainarivo, Luke Dollar, Leon Pierrot Rahajanirina, Radosoa Andrianaivoarivelo, Patricia Parker, Edward Dubovi
Introduced animals impact endemic populations through predation, competition, and disease transmission. Populations of endemic carnivores in Madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. We assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of Madagascar (Kirindy Mitea National Park and Ankarafantsika National Park) and their neighboring villages. We also identified variables associated with the presence of antibodies to these pathogens in fosa ( Cryptoprocta ferox )...
July 2016: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/27154544/canine-circovirus-1-cacv-1-and-canine-parvovirus-2-cpv-2-recurrent-dual-infections-in-a-papillon-breeding-colony
#20
T Thaiwong, A G Wise, R K Maes, T Mullaney, M Kiupel
Recurrent outbreaks of sudden death and bloody diarrhea were reported in March 2013 and February 2014 in a breeding colony of Papillon dogs. During the first outbreak, 1 adult dog and 2 eight-month-old puppies died. During the second outbreak, 2 ten-week-old puppies died. One puppy from the first outbreak and 2 puppies from the second outbreak were examined at necropsy. Histologically, all 3 puppies had severe segmental crypt necrosis of the small intestine and marked lymphoid follicle depletion in the spleen and Peyer's patches...
May 6, 2016: Veterinary Pathology
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