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https://www.readbyqxmd.com/read/28526792/environmental-spread-of-ndm-1-producing-multi-drug-resistant-bacteria-in-dhaka-bangladesh
#1
Mohammad Aminul Islam, Moydul Islam, Rashedul Hasan, M Iqbal Hossain, Ashikun Nabi, Mahdia Rahman, Wil H F Goessens, Hubert P Endtz, Alexandria B Boehm, Shah M Faruque
Resistance to carbapenem antibiotics through the production of New Delhi Metallo-β-lactamase-1 (NDM-1) constitutes an emerging challenge in the treatment of bacterial infections. To monitor possible source of spread of these organisms in Dhaka, Bangladesh, we conducted comparative analysis of waste water samples from hospital adjacent areas (HAR) and from community areas (COM) as well as public tap water samples for the occurrence and characteristics of NDM-1 producing bacteria. Of 72 HAR samples tested 51 (71%) were positive for NDM-1-producing bacteria as evidenced by phenotypic tests and the presence of blaNDM-1 gene, compared to 5 of 41 (12...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526589/a-rapid-salmonella-detection-method-involving-thermophilic-helicase-dependent-amplification-and-a-lateral-flow-assay
#2
Xin-Jun Du, Tian-Jiao Zhou, Ping Li, Shuo Wang
Salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. Since conventional culture methods to detect Salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. In this study, we developed a rapid, simple and portable Salmonella detection strategy that combines thermophilic helicase-dependent amplification (tHDA) with a lateral flow assay to provide a detection result based on visual signals within 90 min...
May 16, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28520983/genomic-epidemiology-of-global-vim-producing-enterobacteriaceae
#3
Yasufumi Matsumura, Gisele Peirano, Rebekah Devinney, Patricia A Bradford, Mary R Motyl, Mark D Adams, Liang Chen, Barry Kreiswirth, Johann D D Pitout
Background: : International data on the molecular epidemiology of Enterobacteriaceae with VIM carbapenemases are limited. Methods: We performed short read (Illumina) WGS on a global collection of 89 VIM-producing clinical Enterobacteriaceae (2008-14). Results: VIM-producing (11 varieties within 21 different integrons) isolates were mostly obtained from Europe. Certain integrons with bla VIM were specific to a country in different species and clonal complexes (CCs) (In 87 , In 624 , In 916 and In 1323 ), while others had spread globally among various Enterobacteriaceae species (In 110 and In 1209 )...
May 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28520867/activity-of-ceftolozane-tazobactam-against-surveillance-and-problem-enterobacteriaceae-pseudomonas-aeruginosa-and-non-fermenters-from-the-british-isles
#4
David M Livermore, Shazad Mushtaq, Daniele Meunier, Katie L Hopkins, Robert Hill, Rachael Adkin, Aiysha Chaudhry, Rachel Pike, Peter Staves, Neil Woodford
Background: We assessed the activity of ceftolozane/tazobactam against consecutive isolates collected in the BSAC Bacteraemia Surveillance from 2011 to 2015 and against 'problem' isolates sent to the UK national reference laboratory from July 2015, when routine testing began. Methods: Susceptibility testing was by BSAC agar dilution with resistance mechanisms identified by PCR and interpretive reading. Results: Data were reviewed for 6080 BSAC surveillance isolates and 5473 referred organisms...
May 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28515389/ca-2-in-hybridization-solutions-for-fluorescence-in-situ-hybridization-facilitates-the-detection-of-enterobacteriaceae
#5
Shin Haruta, Takao Iino, Moriya Ohkuma, Ken-Ichiro Suzuki, Yasuo Igarashi
Fluorescence in situ hybridization (FISH) has been employed to identify microorganisms at the single cell level under a microscope. Extensive efforts have been made to improve and extend the FISH technique; however, the development of a widely applicable protocol is a continuing challenge. The present study evaluated the effects of divalent cations in the hybridization solution on the FISH-based detection of various species of bacteria and archaea with rRNA-targeted probes. A flow cytometric analysis after FISH with a standard hybridization buffer detected positive signals from less than 30% of Escherichia coli IAM 1264 cells...
May 18, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28511293/enterobacter-aerogenes-zdy01-attenuates-choline-induced-trimethylamine-n-oxide-levels-via-remodeling-gut-microbiota-in-mice
#6
Liang Qiu, Dong Yang, Xueying Tao, Jun Yu, Hua Xiong, Hua Wei
Trimethylamine N-oxide (TMAO), which is transformed from trimethylamine (TMA) through hepatic flavin-containing monooxygenases, can promote atherosclerosis. TMA is produced from dietary carnitine, phosphatidylcholine, and choline via the gut microbes. Previous works have shown that some small molecules, such as allicin, resveratrol, and 3,3-dimethyl-1-butanol, are used to reduce circulating TMAO levels. However, the use of bacteria as an effective therapy to reduce TMAO levels has not been reported. In the present study, 82 isolates were screened from healthy Chinese fecal samples on a basal salt medium supplemented with TMA as sole carbon source...
May 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28509939/bioremoval-of-lead-using-pennisetum-purpureum-augmented-with-enterobacter-cloacae-vitpasj1-a-pot-culture-approach
#7
Anamika Das, Priyanka Belgaonkar, Aditya S Raman, Sofia Banu, Jabez W Osborne
Lead is a toxic heavy metal discharged into the ecosystem from various industries. Biological remediation strategies have been effective in the bioremoval of lead. In our current study, a phytobacterial system using Pennisetum purpureum along with lead-resistant bacterium (LRB) was employed for the uptake of lead. The LRB was obtained from lead-contaminated sites. The isolate VITPASJ1 was found to be highly tolerant to lead and was identified as an effective plant growth-promoting bacterium. The 16S rRNA sequencing revealed VITPASJ1 to be the closest neighbour of Enterobacter cloacae...
May 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28508092/a-metabonomic-analysis-on-the-response-of-enterobacter-cloacae-from-coastal-outfall-for-land-based-pollutant-under-phoxim-stress
#8
Dijun Zhang, Shan He, Tinghong Ming, Chenyang Lu, Jun Zhou, Xiurong Su
Enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. The secretion of extended-spectrum β-lactamases (ESBLs) and cephalosporinase (AmpC) endows E. cloacae with strong drug resistance. In a previous study by our group, protein expression of E. cloacae under phoxim stress was measured by two-dimensional electrophoresis. Here, nuclear magnetic resonance was used to detect differences in E. cloacae metabonomics when under phoxim stress. We determined that there are 29 types of metabolites that differ between phoxim stress and normal culture conditions...
May 15, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28507488/microbiological-analysis-of-acute-infections-of-the-nail-fold-on-the-basis-of-bait-thread-test
#9
Hanna Tomczak, Aleksandra Dańczak-Pazdrowska, Adriana Polańska, Agnieszka Osmola-Mańkowska, Jakub Pazdrowski, Wioleta Błażejewska-Gąsior, Anna Horla, Marta Hasse-Cieślińska, Zygmunt Adamski
INTRODUCTION: An acute infection of the nail fold, called paronychia, is a common clinical problem. The basis for the implementation of the treatment is the result of microbiological examination. Due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. AIM: The microbial analysis of acute infections of the nail fold. MATERIAL AND METHODS: The study included 32 tests conducted on 31 patients of the Department of Dermatology...
April 2017: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28506673/high-frequency-of-silver-resistance-genes-in-invasive-isolates-of-enterobacter-and-klebsiella-species
#10
S Sütterlin, M Dahlö, C Tellgren-Roth, W Schaal, Å Melhus
BACKGROUND: Silver-based products have been marketed as an alternative to antibiotics, and their consumption has increased. Bacteria may, however, develop resistance to silver. AIM: To study the presence of genes encoding silver resistance (silE, silP, silS) over time in three clinically important Enterobacteriaceae genera. METHODS: Using polymerase chain reaction (PCR), 752 bloodstream isolates from the years 1990-2010 were investigated. Age, gender, and ward of patients were registered, and the susceptibility to antibiotics and silver nitrate was tested...
April 26, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28501002/effects-of-lactic-acid-bacteria-and-molasses-additives-on-the-microbial-community-and-fermentation-quality-of-soybean-silage
#11
Kuikui Ni, Fangfang Wang, Baoge Zhu, Junxiang Yang, Guoan Zhou, Yi Pan, Yong Tao, Jin Zhong
The objective was to study effects of lactic acid bacteria (L) and molasses (M) on the microbial community and fermentation quality of soybean silage. Soybean was ensiled with no additive control (C), 0.5% molasses (0.5%M), 0.5%M+L (0.5%ML), 2%M, 2%M+L (2%ML) for 7, 14, 30 and 60days. The M-treated silages could increase the content of lactic acid and decrease butyric acid than control. Besides, higher crude protein was also observed in M-treated silages. With prolonged ensiling time, there was a reduction of the ratio of lactic acid/acetic acid in the 2%M-treated and 2%ML-treated silages...
April 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28500888/impact-of-furan-derivatives-and-phenolic-compounds-on-hydrogen-production-from-organic-fraction-of-municipal-solid-waste-using-co-culture-of-enterobacter-aerogenes-and-e-coli
#12
Preeti Sharma, Uma Melkania
In the present study, the effect of furan derivatives (furfural and 5-hydroxymethylfurfural) and phenolic compounds (vanillin and syringaldehyde) on hydrogen production from organic fraction of municipal solid waste (OFMSW) was investigated using co-culture of facultative anaerobes Enterobacter aerogenes and E. coli. The inhibitors were applied in the concentration ranges of 0.25, 0.5, 1, 2 and 5g/L each. Inhibition coefficients of phenolic compounds were higher than those of furan derivatives and vanillin exhibited maximum inhibition coefficients correspondingly lowest hydrogen yield among all inhibitors...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28498924/major-role-of-pkpqil-like-plasmids-in-the-early-dissemination-of-kpc-type-carbapenemases-in-the-uk
#13
M Doumith, J Findlay, H Hirani, K L Hopkins, D M Livermore, A Dodgson, N Woodford
Objectives: Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were first seen in the UK in 2003 and have been increasingly reported since 2010, largely owing to an ongoing outbreak in North-West England. We examined the role of clonal spread and plasmid transmission in their emergence. Methods: : Isolates comprised KPC-positive K. pneumoniae ( n  =   33), Escherichia coli ( n  =   7) and Enterobacter spp. ( n  =   4) referred to the national reference laboratory between 2008 and 2010 from 17 UK centres, including three in North-West England...
May 11, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28491136/applications-of-microalgal-biofilms-for-wastewater-treatment-and-bioenergy-production
#14
Ana F Miranda, Narasimhan Ramkumar, Constandino Andriotis, Thorben Höltkemeier, Aneela Yasmin, Simone Rochfort, Donald Wlodkowic, Paul Morrison, Felicity Roddick, German Spangenberg, Banwari Lal, Sanjukta Subudhi, Aidyn Mouradov
BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28491055/metagenomic-sequencing-of-diamondback-moth-gut-microbiome-unveils-key-holobiont-adaptations-for-herbivory
#15
Xiaofeng Xia, Geoff M Gurr, Liette Vasseur, Dandan Zheng, Huanzi Zhong, Bingcai Qin, Junhan Lin, Yue Wang, FengQin Song, Yong Li, Hailan Lin, Minsheng You
Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28490970/antibacterial-activities-of-the-methanol-extracts-of-albizia-adianthifolia-alchornea-laxiflora-laportea-ovalifolia-and-three-other-cameroonian-plants-against-multi-drug-resistant-gram-negative-bacteria
#16
Cedric F Tchinda, Igor K Voukeng, Veronique P Beng, Victor Kuete
In the last 10 years, resistance in Gram-negative bacteria has been increasing. The present study was designed to evaluate the in vitro antibacterial activities of the methanol extracts of six Cameroonian medicinal plants Albizia adianthifolia, Alchornea laxiflora, Boerhavia diffusa, Combretum hispidum, Laportea ovalifolia and Scoparia dulcis against a panel of 15 multidrug resistant Gram-negative bacterial strains. The broth microdilution was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extracts...
May 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28488226/the-prevalence-of-antibiotic-resistant-bacteria-arb-in-waters-of-the-lower-ballona-creek-watershed-los-angeles-county-california
#17
Stephanie Kawecki, Gary Kuleck, John H Dorsey, Christopher Leary, Michelle Lum
Screening for the prevalence of antibiotic-resistant bacteria (ARB) was done at the Ballona Creek and Wetlands, an urban-impacted wetland system in Los Angeles, California. The goals were (1) to assess the overall prevalence of ARB, and (2) compare differences in ARB abundance and the types of antibiotic resistance (AR) among the following sample types: lagoon water from Del Rey Lagoon, urban runoff from Ballona Creek, and water from the Ballona Wetlands (tidal water flooding in from the adjacent estuary, and ebbing out from the salt marsh)...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28485399/corrigendum-isolation-and-characterization-of-a-bacteriophage-phieap-2-infecting-multidrug-resistant-enterobacter-aerogenes
#18
Erna Li, Xiao Wei, Yanyan Ma, Zhe Yin, Huan Li, Weishi Lin, Xuesong Wang, Chao Li, Zhiqiang Shen, Ruixiang Zhao, Huiying Yang, Aimin Jiang, Wenhui Yang, Jing Yuan, Xiangna Zhao
No abstract text is available yet for this article.
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28484348/the-most-frequent-isolates-from-outpatients-with-urinary-tract-infection
#19
Sabina Mahmutovic Vranic, Nadira Zatric, Velma Rebic, Mufida Aljicevic, Amila Abdulzaimovic
OBJECTIVES: The main objective of the study was to identify the most frequent isolates from female outpatients with urinary tract infection. MATERIAL AND METHODS: The retrospective study was performed at the Department of Microbiology, Parasitology and Virology Faculty of Medicine, University of Sarajevo in period of 2015. The most frequent isolates were determined in 405 first urine samples from female outpatients of Hrasno community in Sarajevo, B&H. Identification of isolates was performed by the standard microbiological testing and the standard methods of descriptive statistics as well...
March 2017: Materia Socio-medica
https://www.readbyqxmd.com/read/28477003/green-synthesis-of-silver-nanoparticles-from-grape-and-tomato-juices-and-evaluation-of-biological-activities
#20
Muhammad Zia, Shadab Gul, Javed Akhtar, Ihsan Ul Haq, Bilal Haider Abbasi, Ahsan Hussain, Sania Naz, Muhammad Fayyaz Chaudhary
The biosynthesis of silver nanoparticles (AgNPs) is substantial for its application in lots of fields. Tomato and grape fruit juices were used as a reducing and capping agents for the biosynthesis of AgNPs. Ultraviolet spectroscopic analysis offered peaks in the range of 396‒420 nm that indicate the production of AgNPs. Fourier transform infrared spectroscopy analysis revealed attachment of different functional groups with Ag ion in both tomato and grape fruit extracts NPs. The X‒ray diffraction analysis confirmed that the synthesised AgNPs have a face centred cubic confirmation...
March 2017: IET Nanobiotechnology
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