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https://www.readbyqxmd.com/read/29331370/preparation-and-evaluation-of-antibacterial-potential-of-pithecellobium-dulce-root-extract-against-gram-positive-and-gram-negative-bacteria
#1
Muneer Ahmad Bhat, Rayees Ahmad Malik, Poonam Prakash, Ali Mohd Lone
In the present study hexane, benzene, ethyl acetate and ethanol extracts of Pithecellobium dulce root were prepared using soxhlet extractor. The extracts were evaluated for antibacterial activity against one Gram positive (Staphylococcus aureus) and three Gram negative (Acetobacter aceti, Acetobacter aceti, Klebsiella pneumoniae) strains. Disc diffusion method revealed promising antibacterial activity of the extracts prepared in polar solvents (ethyl acetate and ethanol) compared to non-polar solvents (hexane and benzene)...
January 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29330994/pattern-of-causative-micro-organisms-in-catheter-related-blood-stream-infections-in-dialysis-patients-experience-from-saudi-arabia
#2
Bilal Mohsin
BACKGROUND: Catheter related blood stream infections (CRBSI) are the leading cause of morbidity in HD patients. The majority of these infections relate to haemodialysis catheters. There is a paucity of local data on microbial agents responsible for CRBSI in our region. This prompted our study. METHODS: This Prospective observatory survey was conducted in Department of Nephrology, King Fahd Hospital, Hofuf KSA from Nov 2014 to Jan 2017 (26 months). It was performed on dialysis patients with HD catheters who developed features of CRBSI...
October 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29330592/genomic-characterization-of-key-bacteriophages-to-formulate-the-potential-biocontrol-agent-to-combat-enteric-pathogenic-bacteria
#3
Krupa M Parmar, Nishant A Dafale, Hitesh Tikariha, Hemant J Purohit
Combating bacterial pathogens has become a global concern especially when the antibiotics and chemical agents are failing to control the spread due to its resistance. Bacteriophages act as a safe biocontrol agent by selectively lysing the bacterial pathogens without affecting the natural beneficial microflora. The present study describes the screening of prominent enteric pathogens NDK1, NDK2, NDK3, and NDK4 (Escherichia, Klebsiella, Enterobacter, and Serratia) mostly observed in domestic wastewater; against which KNP1, KNP2, KNP3, and KNP4 phages were isolated...
January 12, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29320234/influence-of-soap-characteristics-and-food-service-facility-type-on-the-degree-of-bacterial-contamination-of-open-refillable-bulk-soaps
#4
Donald W Schaffner, Dane Jensen, Charles P Gerba, David Shumaker, James W Arbogast
Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29318401/synthesis-anti-microbial-activity-cytotoxicity-of-some-novel-substituted-5-3-1h-benzo-d-imidazol-2-yl-4-hydroxybenzyl-benzofuran-2-yl-phenyl-methanone-analogs
#5
Bhookya Shankar, Pochampally Jalapathi, Balabadra Saikrishna, Shaym Perugu, Vijjulatha Manga
BACKGROUND: There is a dire need for the discovery and development of new antimicrobial agents after several experiments for a better resistance of microorganisms towards antimicrobial agents become a serious health problem for a few years in the past. As benzimidazole possess various types of biological activities, it has been synthesized, in the present study, a new series of (5-(3-(1H-benzo[d]imidazol-2-yl)-4-hydroxybenzyl)benzofuran-2-yl)(phenyl)methanone analogs by using the condensation and screened for its in vitro antimicrobial activity and cytotoxicity...
January 9, 2018: Chemistry Central Journal
https://www.readbyqxmd.com/read/29317709/increased-abundance-of-clostridium-and-fusobacterium-in-gastric-microbiota-of-patients-with-gastric-cancer-in-taiwan
#6
Yung-Yu Hsieh, Shui-Yi Tung, Hung-Yu Pan, Chih-Wei Yen, Huang-Wei Xu, Ying-Jhen Lin, Yi-Fang Deng, Wan-Ting Hsu, Cheng-Shyong Wu, Chin Li
Helicobacter pylori is recognised as a main risk factor for gastric cancer. However, approximately half of the patients with gastritis are negative for H. pylori infection, and the abundance of H. pylori decreases in patients with cancer. In the current study, we profiled gastric epithelium-associated bacterial species in patients with gastritis, intestinal metaplasia, and gastric cancer to identify additional potential pathogenic bacteria. The overall composition of the microbiota was similar between the patients with gastritis and those with intestinal metaplasia...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29311457/-intestinal-microbiota-in-migrating-barn-swallows-around-osaka
#7
Takehiko Kenzaka, Kenji Kataoka, Takashi Fujimitsu, Katsuji Tani
 Migratory birds are considered as vectors of infectious diseases, owing to their potential for transmitting pathogens over large distances. The populations of barn swallow (Hirundo rustica) migrate from Southeast Asia to the Japanese mainland during spring and migrate back to Southeast Asia during autumn. This migratory population is estimated to comprise approximately hundreds to thousands of individuals per year. However, to date, not much is known about the gastrointestinal microbiota of the barn swallow...
2018: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/29311089/molecular-characterization-of-imp-1-producing-enterobacter-cloacae-complex-isolates-in-tokyo
#8
Kotaro Aoki, Sohei Harada, Koji Yahara, Yoshikazu Ishii, Daisuke Motooka, Shota Nakamura, Yukihiro Akeda, Tetsuya Iida, Kazunori Tomono, Satoshi Iwata, Kyoji Moriya, Kazuhiro Tateda
Although KPC enzymes are most common among carbapenemases produced by Enterobacter cloacae complex globally, the epidemiology varies from one country to another. While previous studies have suggested that IMP enzymes are most common in Japan, detailed analysis has been scarce thus far. Here we carried out a molecular epidemiological study and plasmid analysis of IMP-1-producing E. cloacae complex isolates collected from 3 hospitals in central Tokyo using whole genome sequencing. Seventy-one isolates were classified into several STs and 49 isolates were identified as Enterobacter hormaechei ST78...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29311088/antibiotic-resistance-plasmids-cointegrated-into-a-megaplasmid-harbouring-the-blaoxa-427-carbapenemase-gene
#9
Stefanie Desmet, Suruchi Nepal, Jan Maarten van Dijl, Marc Van Ranst, Monika A Chlebowicz, John W Rossen, Jeroen K J Van Houdt, Piet Maes, Katrien Lagrou, Erik Bathoorn
OXA-427 is a new class-D carbapenemase encountered in different species of Enterobacteriaceae in a Belgian hospital. To study the dispersal of this gene, we describe the comparative analysis of two plasmids containing the blaOXA-427 gene isolated from a Klebsiella pneumoniae and an Enterobacter cloacae complex strain. The two IncA/C2 plasmids containing blaOXA-427 share the same backbone, however, in the K. pneumoniae this plasmid is cointegrated into an IncFIB plasmid forming a 321 kb megaplasmid with multiple multi-resistance regions...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29304717/analysis-of-the-spectrum-and-antibiotic-resistance-of-uropathogens-in-outpatients-at-a-tertiary-hospital
#10
Bo Yang, Fujia Yang, Shanmei Wang, Qian Wang, Zhirui Liu, Wei Feng, Fengjun Sun, Peiyuan Xia
The objective of this study was to analyse the distribution and antimicrobial resistance of bacterial uropathogens isolated from outpatients at Henan Provincial People's Hospital. A total of 1419 samples from 823 newly diagnosed and 596 recurrent UTI outpatients culture positive. Escherichia coli was the most common uropathogen. Compared with the recurrent group, the newly diagnosed group had a higher isolation rate of E. coli and Enterobacter cloacae but a lower isolation rate of Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp...
January 5, 2018: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29301895/whole-genome-sequence-of-the-novel-temperate-enterobacter-bacteriophage-tyrion-isolated-from-the-gut-of-the-formosan-subterranean-termite
#11
Chinmay Vijay Tikhe, Chris R Gissendanner, Claudia Husseneder
We sequenced the genome of the novel Enterobacter bacteriophage Tyrion isolated from termite gut. The bacteriophage is temperate in nature and belongs to the Podoviridae family. Many predicted bacteriophage genes showed similarity to prophage regions of the genomes of Enterobacteriaceae bacteria. This is the second bacteriophage isolated from termite gut.
January 4, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29301894/whole-genome-sequence-of-the-novel-enterobacter-bacteriophage-arya-with-an-integrase-pseudogene-isolated-from-the-gut-of-the-formosan-subterranean-termite
#12
Chinmay Vijay Tikhe, Chris R Gissendanner, Claudia Husseneder
We isolated and sequenced the novel Enterobacter bacteriophage Arya from termite gut. The genome showed synteny to lytic bacteriophage genomes; however, the genome encodes a truncated, putatively nonfunctional integrase pseudogene. Lysogeny-related genes were previously observed in certain lytic phages, but their role and evolution remain unclear.
January 4, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29298099/propidium-monoazide-quantitative-real-time-polymerase-chain-reaction-for-enumeration-of-some-viable-but-nonculturable-foodborne-bacteria-in-meat-and-meat-products
#13
Norhan Khairy Abd El-Aziz, Yasmine Hasanine Tartor, Ahlam Abd El-Aziz Gharib, Ahmed Mohamed Ammar
Foodborne infections due to bacterial pathogens are increasing worldwide. Given the surreptitious nature of viable but nonculturable (VBNC) bacteria, they largely remain a threat to public health and food safety due to their non-detectability through conventional plate count techniques. Hence, species-specific quantitative real-time polymerase chain reaction (PCR) (qPCR) alone and combined with the use of propidium monoazide (PMA) was used along with the plate count method to quantify VBNC Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, and Enterobacteriaceae in fresh and processed meat samples...
January 3, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29297851/ram-locus-is-a-key-regulator-to-trigger-multidrug-resistance-in-enterobacter-aerogenes
#14
Alexander Molitor, Chloë E James, Séamus Fanning, Jean-Marie Pagès, Anne Davin-Regli
PURPOSE: Several genetic regulators belonging to AraC family are involved in the emergence of MDR isolates of E. aerogenes due to alterations in membrane permeability. Compared with the genetic regulator Mar, RamA may be more relevant towards the emergence of antibiotic resistance. METHODOLOGY: Focusing on the global regulators, Mar and Ram, we compared the amino acid sequences of the Ram repressor in 59 clinical isolates and laboratory strains of E. aerogenes. Sequence types were associated with their corresponding multi-drug resistance phenotypes and membrane protein expression profiles using MIC and immunoblot assays...
January 3, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29297794/the-eye-of-the-azara-s-agouti-dasyprocta-azarae-morphological-observations-and-selected-ophthalmic-diagnostic-tests
#15
André Tavares-Somma, Nathália Seabra, Bret A Moore, Mario Sato, Rogério R Lange, Fabiano Montiani-Ferreira
The purpose of this study was to carry out a descriptive investigation of the Azara's agouti ( Dasyprocta azarae) eye and to establish reference values of select ophthalmic diagnostic tests and physiologic parameters. A total of 19 healthy agoutis were used. Select ophthalmic diagnostic tests were performed, including Schirmer tear test type I (STTI), analysis of the conjunctival bacterial microflora, corneal esthesiometry, and tonometry. B-mode ultrasonic biometry, fundus photography, optical coherence tomography, and gross and histologic analysis of two eyes were also performed...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29286016/granulated-sugar-for-adjuvant-treatment-of-surgical-wound-infection-due-to-multi-drug-resistant-pathogens-in-a-child-with-sarcoma-a-case-report-and-literature-review
#16
Aldo Naselli, Laura Accame, Piero Buffa, Anna Loy, Roberto Bandettini, Alberto Garaventa, Ornella Della Casa Alberighi, Elio Castagnola
The use of sugar for treating wounds which are difficult to heal and positive to resistant pathogens has already been documented. The authors describe the successful treatment by direct instillation of granular sugar in the antibiotic-resistant infected surgical site wound of a child with sarcoma. Sugar instillation in the extended spectrum beta-lactamase (ESBL)-producing Enterobacter cloacae positive wound, in addition to systemic treatment with meropenem and levofloxacin, allowed culture negativization in six days and complete wound healing in 30 days...
December 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/29286015/prevalence-of-class-1-integrons-and-plasmid-mediated-qnr-genes-among-enterobacter-isolates-obtained-from-hospitalized-patients-in-ahvaz-iran
#17
Mansour Amin, Solmaz Dibachi, Mojtaba Shahin
Quinolones are frequently used classes of antimicrobials in hospitals, crucial for the treatment of infections caused by Gram-negative bacteria. The inappropriate use of quinolones and other antimicrobial agents for the treatment of bacterial infections leads to a significant increase of resistant isolates. The acquisition of antimicrobial resistance may be related to achievement of resistance determinant genes mediated by plasmids, transposons and gene cassettes in integrons. The objective of this cross-sectional study, conducted from December 2015 to July 2016 at two teaching hospitals in Ahvaz, southern Iran, was to screen for the presence of class 1 integrons and quinolone resistance genes in clinical isolates of Enterobacter spp...
December 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/29286008/update-on-the-main-mdr-pathogens-prevalence-and-treatment-options
#18
Silvano Esposito, Giuseppe De Simone
In recent years the proportion of multi-drug resistance (MDR) among the bacterial pathogens causing infections, particularly those acquired in healthcare settings, has risen worryingly worldwide. It poses a serious public health threat as the multiple patterns of resistance limit the effective treatment options for such infections. Although many bacterial species have developed reduced susceptibility to a wide array of antimicrobial molecules, a particular group of pathogens acronymically referred to as ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp...
December 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/29284535/bacterial-diversity-of-wild-caught-lutzomyia-longipalpis-a-vector-of-zoonotic-visceral-leishmaniasis-in-brazil-under-distinct-physiological-conditions-by-metagenomics-analysis
#19
Ana Clara Araújo Machado Pires, Luís Eduardo Martinez Villegas, Thaís Bonifácio Campolina, Alessandra Silva Orfanó, Paulo Filemon Paolucci Pimenta, Nágila Francinete Costa Secundino
BACKGROUND: The leishmaniases are a group of diseases caused by protozoans of the genus Leishmania, which are transmitted by the bite of phlebotomine sand flies. In the New World, Lutzomyia longipalpis is the most important vector of visceral leishmaniasis and is a proven vector for Leishmania infantum chagasi in Brazil. During development within the vector, Leishmania can interact with a variety of microorganisms such as fungi and bacteria. The presence of bacteria in the midgut of sand flies can influence the development and survival of the parasite...
December 29, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29279516/spontaneous-development-of-acute-obstructive-suppurative-pancreatic-ductitis-associated-with-pancreatic-carcinoma-a-first-case-report
#20
Kunio Iwatsuka, Hiroshi Nakagawara, Masahiro Ogawa, Takuji Gotoda, Shigeoki Hayashi, Noriko Kinukawa, Akihiro Hemmi, Kenji Yamao, Akio Yanagisawa, Mitsuhiko Moriyama
A 68-year-old man with a history of diabetes mellitus was admitted to our hospital with a diagnosis of acute pancreatitis. Abdominal computed tomography revealed a suspicious tumor in the body of the pancreas, along with a dilated main pancreatic duct and edema of the pancreatic tail. Endoscopic retrograde pancreatography was performed after treating the patient's pancreatitis. When a cannula tip was advanced beyond the stenosis, deep into the distal pancreatic duct, thick white pus was evacuated. A bacteriological examination of the aspirated pancreatic juice revealed Enterobacter cloacae, and a cytological examination revealed adenocarcinoma...
December 27, 2017: Internal Medicine
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