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https://www.readbyqxmd.com/read/29777263/folate-treatment-of-pregnant-rat-dams-abolishes-metabolic-effects-in-female-offspring-induced-by-a-paternal-pre-conception-unhealthy-diet
#1
Jian Li, Yong-Ping Lu, Oleg Tsuprykov, Ahmed A Hasan, Christoph Reichetzeder, Mei Tian, Xiao Li Zhang, Qin Zhang, Guo-Ying Sun, Jingli Guo, Mohamed M S Gaballa, Xiao-Ning Peng, Ge Lin, Berthold Hocher
AIMS/HYPOTHESIS: Paternal high-fat diet prior to mating programmes impaired glucose tolerance in female offspring. We examined whether the metabolic consequences in offspring could be abolished by folate treatment of either the male rats before mating or the corresponding female rats during pregnancy. METHODS: Male F0 rats were fed either control diet or high-fat, high-sucrose and high-salt diet (HFSSD), with or without folate, before mating. Male rats were mated with control-diet-fed dams...
May 18, 2018: Diabetologia
https://www.readbyqxmd.com/read/29775429/genome-wide-dna-methylation-profiles-of-porcine-ovaries-in-estrus-and-proestrus
#2
Xiaolong Zhou, Songbai Yang, Feifei Yan, Ke He, Ayong Zhao
DNA methylation is an important epigenetic modification involved in the estrous cycle and the regulation of reproduction. Here, we investigated the genome-wide profiles of DNA methylation in porcine proestrus and estrus ovaries using methylated DNA immunoprecipitation sequencing (MeDIP-seq). The results showed that DNA methylation was enriched in intergenic and intron regions. The methylation levels of coding regions were higher than those of the 5' and 3' flanking regions of genes. There were 4813 differentially methylated regions (DMRs) of CpG islands in the estrus vs...
May 18, 2018: Physiological Genomics
https://www.readbyqxmd.com/read/29767751/clinicopathological-and-molecular-differences-between-right-sided-and-left-sided-colorectal-cancer-in-japanese-patients
#3
Soichiro Natsume, Tatsuro Yamaguchi, Misato Takao, Takeru Iijima, Rika Wakaume, Keiichi Takahashi, Hiroshi Matsumoto, Daisuke Nakano, Shin-Ichiro Horiguchi, Koichi Koizumi, Michiko Miyaki
Background: The aim of this study was to clarify clinicopathological features, frequencies of molecular biomarkers, and prognoses in Japanese colorectal cancer patients and compare them with right-sided colon cancer (RCC) and left-sided colorectal cancer (LCRC). Methods: We consecutively selected 575 colorectal cancer patients who underwent surgical resection from 2008 to 2011. RCC was located from the cecum to the transverse colon, and LCRC was located from the splenic flexure to the rectum...
May 15, 2018: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29762634/lineage-associated-underrepresented-permutations-laups-of-mammalian-genomic-sequences-based-on-a-jellyfish-based-laups-analysis-application-jbla
#4
Le Zhang, Ming Xiao, Jingsong Zhou, Jun Yu
Motivation: The present study addresses several important questions related to naturally underrepresented sequences: (1) Are there permutations of real genomic DNA sequences in a defined length (k-mer) and a given lineage that do not actually exist or underrepresented? (2) If there are such sequences, what are their characteristics in terms of k-mer length and base composition? Are they related to CpG or TpA underrepresentation known for human sequences? We propose that the answers to these questions are of great significance for the study of sequence-associated regulatory mechanisms, such cytosine methylation and chromosomal structures in physiological or pathological conditions such as cancer...
May 14, 2018: Bioinformatics
https://www.readbyqxmd.com/read/29762087/genomics-and-epigenomics-of-congenital-heart-defects-expert-review-and-lessons-learned-in-africa
#5
Nicholas Ekow Thomford, Kevin Dzobo, Nana Akyaa Yao, Emile Chimusa, Jonathan Evans, Emmanuel Okai, Paul Kruszka, Maximilian Muenke, Gordon Awandare, Ambroise Wonkam, Collet Dandara
Congenital heart defects (CHD) are structural malformations found at birth with a prevalence of 1%. The clinical trajectory of CHD is highly variable and thus in need of robust diagnostics and therapeutics. Major surgical interventions are often required for most CHDs. In Africa, despite advances in life sciences infrastructure and improving education of medical scholars, the limited clinical data suggest that CHD detection and correction are still not at par with the rest of the world. But the toll and genetics of CHDs in Africa has seldom been systematically investigated...
May 2018: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/29761860/hypermethylation-of-cpg-islands-is-associated-with-increasing-chromosomal-damage-in-chinese-lead-exposed-workers
#6
Li-Bo Yu, Yu-Ting Tu, Jing-Wen Huang, Ya-Nan Zhang, Guo-Qiao Zheng, Xiao-Wen Xu, Jin-Wei Wang, Jean Qin Xiao, David C Christiani, Zhao-Lin Xia
Lead is a widely existing environmental pollutant with potential carcinogenicity. To investigate the association of blood lead level (B-Pb) with potential chromosomal damage and cancer, we analyzed micronucleus (MN) frequency of peripheral blood lymphocytes (PBLs) and the methylation status of six human tumor suppressor genes (TSGs) post lead exposure. In the study, 147 lead-exposed workers were divided into two groups according to their B-Pb P50 value, with other 50 lead-unexposed workers as a control group...
May 15, 2018: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29759990/epigenetic-targeting-of-adipocytes-inhibits-high-grade-serous-ovarian-cancer-cell-migration-and-invasion
#7
Jessica Tang, Nicholas Pulliam, Ali Ozes, Aaron Buechlein, Ning Ding, Doug Rusch, Harold Keer, Heather O'Hagan, M Sharon Stack, Kenneth P Nephew
Ovarian cancer (OC) cells frequently metastasize to the omentum and adipocytes play a significant role in ovarian tumor progression. Therapeutic interventions targeting aberrant DNA methylation in ovarian tumors have shown promise in the clinic but the effects of epigenetic therapy on the tumor microenvironment are understudied. Here, we examined the effect of adipocytes on OC cell behavior in culture and impact of targeting DNA methylation in adipocytes on OC metastasis. The presence of adipocytes increased OC cell migration and invasion and proximal and direct co-culture of adipocytes increased OC proliferation alone or after treatment with carboplatin...
May 14, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29757674/eicosapentaenoic-acid-induces-dna-demethylation-in-carcinoma-cells-through-a-tet1-dependent-mechanism
#8
Veronica Ceccarelli, Virginia Valentini, Simona Ronchetti, Lorenza Cannarile, Monia Billi, Carlo Riccardi, Laura Ottini, Vincenzo Nicola Talesa, Francesco Grignani, Alba Vecchini
In cancer cells, global genomic hypomethylation is found together with localized hypermethylation of CpG islands within the promoters and regulatory regions of silenced tumor suppressor genes. Demethylating agents may reverse hypermethylation, thus promoting gene re-expression. Unfortunately, demethylating strategies are not efficient in solid tumor cells. DNA demethylation is mediated by ten-eleven translocation enzymes (TETs). They sequentially convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which is associated with active transcription; 5-formylcytosine; and finally, 5-carboxylcytosine...
May 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29755768/detecting-promoter-methylation-pattern-of-apoptotic-genes-apaf1-and-caspase8-in-gastric-carcinoma-patients-undergoing-chemotherapy
#9
Fatemeh Azarkhazin, Golnaz Asaadi Tehrani
Background: DNA methylation patterns in cells dysregulation CpG island methylation of genes involved in cancer leads to increased levels of the cancer. Restoration of the apoptotic route in tumor cells of stomach in order for placing Casp8 and Apaf1 genes is a proper approach for new treatments of gastric cancer. The objective of the present study was to investigate the relationship between the pattern of methylation promoter in apoptotic genes of Casp8 and Apaf1 and gastric carcinoma in patients receiving chemotherapy...
April 2018: Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/29750576/suv39h1-dnmt3a-dependent-methylation-of-the-rb1-promoter-stimulates-pin1-expression-and-melanoma-development
#10
Garam Kim, Jin-Young Kim, Sung-Chul Lim, Kwang Youl Lee, Okyun Kim, Hong Seok Choi
Melanoma is among the most aggressive and treatment-resistant human cancers. Aberrant histone H3 methylation at Lys 9 (H3K9) correlates with carcinogenic gene silencing, but the significance of suppressor of variegation 3-9 homolog 1 (SUV39H1), an H3K9-specific methyltransferase, in melanoma initiation and progression remains unclear. Here, we show that SUV39H1-mediated H3K9 trimethylation facilitates retinoblastoma ( RB) 1 promoter CpG island methylation by interacting with DNA methyltransferase 3A and decreasing RB mRNA and protein in melanoma cells...
May 11, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29747491/gastric-mucosal-atrophy-impedes-housekeeping-gene-methylation-in-gastric-cancer-patients
#11
Jung-Hwan Oh, Mun-Gan Rhyu, Suk-Il Kim, Mi-Ri Yun, Jung-Ha Shin, Seung-Jin Hong
Purpose: Helicobacter pylori infection induces phenotype-stabilizing methylation and promotes gastric mucosal atrophy that can inhibit CpG-island methylation. Relationship between the progression of gastric mucosal atrophy and the initiation of CpG-island methylation was analyzed to delineate epigenetic period for neoplastic transformation. Materials and Methods: Normal-appearing gastric mucosa was biopsied from 110 H. pylori‒positive controls, 95 H. pylori‒negative controls, 99 gastric cancer patients, and 118 gastric dysplasia patients...
April 30, 2018: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/29745077/association-between-promoter-hypermethylation-of-the-dact2-gene-and-tumor-stages-in-breast-cancer
#12
Veronica Marusa Borgonio-Cuadra, Antonio Miranda-Duarte, Xochitl Rojas-Toledo, Normand Garcia-Hernandez, Jose Alfredo Sierra-Ramirez, Maura Cardenas-Garcia, Marta Elena Hernandez-Caballero
PURPOSE: Aberrant methylation of CpG islands in the promoter is a hallmark of cancer, leading to transcriptional silencing of tumor suppressor genes. The aim of this work was to evaluate the promoter methylation status of the DACT2 gene in breast cancer (BC) tissue and to analyze its possible effect on tumor type or grade. METHODS: CpG island from the DACT2 promoter in region -240 to -14 from transcriptional start site (TSS) were obtained. Through the use of sodium bisulfite DNA conversion analysis, followed by detection with MSP (methylation specific PCR), we analyzed 79 BC and 15 adjacent healthy samples...
March 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29743854/microsatellite-instability-in-colorectal-cancer
#13
REVIEW
Jafar Nouri Nojadeh, Shahin Behrouz Sharif, Ebrahim Sakhinia
Colorectal cancer (CRC) is a heterogeneous disease that is caused by the interaction of genetic and environmental factors. Although it is one of the most common cancers worldwide, CRC would be one of the most curable cancers if it is detected in the early stages. Molecular changes that occur in colorectal cancer may be categorized into three main groups: 1) Chromosomal Instability (CIN), 2) Microsatellite Instability (MSI), and 3) CpG Island Methylator phenotype (CIMP). Microsatellites, also known as Short Tandem Repeats (STRs) are small (1-6 base pairs) repeating stretches of DNA scattered throughout the entire genome and account for approximately 3 % of the human genome...
2018: EXCLI Journal
https://www.readbyqxmd.com/read/29743579/analysis-of-early-changes-in-dna-methylation-in-synovial-fibroblasts-of-ra-patients-before-diagnosis
#14
Emmanuel Karouzakis, Karim Raza, Christoph Kolling, Christopher D Buckley, Steffen Gay, Andrew Filer, Caroline Ospelt
DNA methylation is an important epigenetic modification that is known to be altered in rheumatoid arthritis synovial fibroblasts (RASF). Here, we compared the status of promoter DNA methylation of SF from patients with very early RA with SF from patients with resolving arthritis, fully established RA and from non-arthritic patients. DNA was hybridized to Infinium Human methylation 450k and 850k arrays and differential methylated genes and pathways were identified. We could identify a significant number of CpG sites that differed between the SF of different disease stages, showing that epigenetic changes in SF occur early in RA development...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29739406/dna-methylation-in-the-apoe-genomic-region-is-associated-with-cognitive-function-in-african-americans
#15
Jiaxuan Liu, Wei Zhao, Erin B Ware, Stephen T Turner, Thomas H Mosley, Jennifer A Smith
BACKGROUND: Genetic variations in apolipoprotein E (APOE) and proximal genes (PVRL2, TOMM40, and APOC1) are associated with cognitive function and dementia, particularly Alzheimer's disease. Epigenetic mechanisms such as DNA methylation play a central role in the regulation of gene expression. Recent studies have found evidence that DNA methylation may contribute to the pathogenesis of dementia, but its association with cognitive function in populations without dementia remains unclear...
May 8, 2018: BMC Medical Genomics
https://www.readbyqxmd.com/read/29737090/-expression-of-asph-gene-in-invasive-breast-cancer-and-its-clinical-significance-in-promoter-methylation
#16
Chuan Li, Hong-Jiang Li, Qu Chen, Xue-Mei Zhang, Chang-Long Li
OBJECTIVE: To investigate the expression of mRNA of aspartyl/asparaginyl beta-hydroxylase (ASPH) gene in invasive breast cancer (IBC) and the relationship between methylation of gene promoter and clinicopathological parameters. METHODS: In 91 cases of breast cancer tissues and matched normal tissues (MNT),mRNA expression of the ASPH gene was detected by reverse transcription of real-time fluorescence quantification PCR and the methylation status of CpG island in the ASPH gene promoter region was detected by methylation specific PCR (MSP)...
January 2018: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/29733698/age-associated-increase-of-thyroid-hormone-receptor-%C3%AE-gene-promoter-methylation-coexists-with-decreased-gene-expression
#17
Eliza Pawlik-Pachucka, Monika Budzinska, Zofia Wicik, Anna Domaszewska-Szostek, Magdalena Owczarz, Malgorzata Roszkowska-Gancarz, Magdalena Gewartowska, Monika Puzianowska-Kuznicka
PURPOSE: It is not established if healthy aging of the thyroid axis is associated with alterations other than changes in hormone secretion. METHODS: The expression of thyroid hormone receptor β gene (THRB) was analyzed in peripheral blood mononuclear cells (PBMC) obtained from young, elderly, and long-lived individuals. The interaction between the 3'UTR of TRβ1 mRNA and selected miRNAs was measured using pmirGLO reporter vector. Methylation of the THRB CpG island was analyzed using methylation-sensitive restriction/RT-PCR and bisulfite sequencing methods...
May 7, 2018: Endocrine Research
https://www.readbyqxmd.com/read/29730775/dna-methylation-based-reclassification-of-olfactory-neuroblastoma
#18
David Capper, Nils W Engel, Damian Stichel, Matt Lechner, Stefanie Glöss, Simone Schmid, Christian Koelsche, Daniel Schrimpf, Judith Niesen, Annika K Wefers, David T W Jones, Martin Sill, Oliver Weigert, Keith L Ligon, Adriana Olar, Arend Koch, Martin Forster, Sebastian Moran, Oscar M Tirado, Miguel Sáinz-Japeado, Jaume Mora, Manel Esteller, Javier Alonso, Xavier Garcia Del Muro, Werner Paulus, Jörg Felsberg, Guido Reifenberger, Markus Glatzel, Stephan Frank, Camelia M Monoranu, Valerie J Lund, Andreas von Deimling, Stefan Pfister, Rolf Buslei, Julika Ribbat-Idel, Sven Perner, Volker Gudziol, Matthias Meinhardt, Ulrich Schüller
Olfactory neuroblastoma/esthesioneuroblastoma (ONB) is an uncommon neuroectodermal neoplasm thought to arise from the olfactory epithelium. Little is known about its molecular pathogenesis. For this study, a retrospective cohort of n = 66 tumor samples with the institutional diagnosis of ONB was analyzed by immunohistochemistry, genome-wide DNA methylation profiling, copy number analysis, and in a subset, next-generation panel sequencing of 560 tumor-associated genes. DNA methylation profiles were compared to those of relevant differential diagnoses of ONB...
May 5, 2018: Acta Neuropathologica
https://www.readbyqxmd.com/read/29728108/decitabine-demonstrates-antileukemic-activity-in-b-cell-precursor-acute-lymphoblastic-leukemia-with-mll-rearrangements
#19
C Roolf, A Richter, C Konkolefski, G Knuebel, A Sekora, S Krohn, J Stenzel, B J Krause, B Vollmar, H Murua Escobar, C Junghanss
BACKGROUND: Promotor hypermethylation of CpG islands is common in B cell precursor acute lymphoblastic leukemia (BCP-ALL) with mixed lineage leukemia (MLL) gene rearrangements. Hypomethylating agents (HMA) such as azacitidine (AZA) and decitabine (DEC) reduce DNA hypermethylation by incorporation into DNA and were successfully introduced into the clinic for the treatment of myeloid neoplasias. METHODS: Here, we investigated whether HMA induce comparable biological effects in MLL-positive BCP-ALL...
May 4, 2018: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/29721644/morphology-oriented-epigenetic-research
#20
REVIEW
Sohei Kitazawa, Ryuma Haraguchi, Riko Kitazawa
Cytosine methylation plays a major role in the regulation of sequential and tissue-specific expression of genes. De novo aberrant DNA methylation and demethylation are also crucial processes in tumorigenesis and tumor progression. The mechanisms of how and when such aberrant methylation and demethylation occur in tumor cells are still obscure, however. To evaluate subtle epigenetic alteration among minor subclonal populations, morphology-oriented epigenetic analysis is requisite, especially where heterogeneity and flexibility are as notable as in the process of cancer progression and cellular differentiation at critical stages...
May 2, 2018: Histochemistry and Cell Biology
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