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Yun-Jian Cheng, Wenge Qiu, Jin Lei
Tunnel lining (bare-lining) cross-sections play an important role in analyzing deformations of tunnel linings. The goal of this paper is to develop an automatic method for extracting bare-lining cross-sections from terrestrial laser scanning (TLS) point clouds. First, the combination of a 2D projection strategy and angle criterion is used for tunnel boundary point detection, from which we estimate the two boundary lines in the X-Y plane. The initial direction of the cross-sectional plane is defined to be orthogonal to one of the two boundary lines...
October 6, 2016: Sensors
Xuan Cao, Yu-Bei Lv, Jun Chen, Tadayuki Imanaka, Liu-Jing Wei, Qiang Hua
BACKGROUND: Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from essential oils or as a byproduct of orange juice production, however, considering the increasing need for limonene and a certain amount of pesticides may exist in the limonene obtained from the citrus industry, some other methods should be explored to produce limonene. RESULTS: To construct the limonene synthetic pathway in Yarrowia lipolytica, two genes encoding neryl diphosphate synthase 1 (NDPS1) and limonene synthase (LS) were codon-optimized and heterologously expressed in Y...
2016: Biotechnology for Biofuels
Rachael A Smith, Michael St J Warne, Kerrie Mengersen, Ryan D R Turner
Pollutant loads are widely used to set pollution reduction targets and assess regulatory compliance for the protection of receiving waterbodies. However, when a pollutant load is comprised of a mixture of chemicals, reducing the overall load (mass) will not necessarily reduce the toxicity by a similar amount. This can be overcome by setting targets based on toxicity-based loads (toxic loads), where the load is modified according to the relative toxicity (expressed as toxic equivalency factors- TEFs) of each toxicant...
October 24, 2016: Integrated Environmental Assessment and Management
Mark Hedglin, Binod Pandey, Stephen J Benkovic
Translesion DNA synthesis (TLS) during S-phase uses specialized TLS DNA polymerases to replicate a DNA lesion, allowing stringent DNA synthesis to resume beyond the offending damage. Human TLS involves the conjugation of ubiquitin to PCNA clamps encircling damaged DNA and the role of this post-translational modification is under scrutiny. A widely-accepted model purports that ubiquitinated PCNA recruits TLS polymerases such as pol η to sites of DNA damage where they may also displace a blocked replicative polymerase...
October 22, 2016: ELife
Wenyan Xu, Daniel Kool, Derek K O'Flaherty, Ashley Keating, Lauralicia Sacre, Martin Egli, Anne Marietta Noronha, Christopher James Wilds, Linlin Zhao
DNA interstrand cross-links (ICLs) are cytotoxic DNA lesions derived from reactions of DNA with a number of anti-cancer reagents as well as endogenous bifunctional electrophiles. Deciphering the DNA repair mechanisms of ICLs is important for understanding the toxicity of DNA cross-linking agents and for the development of effective chemotherapies. Previous research has focused on ICLs cross-linked with the N7 and N2 atoms of guanine as well as those formed at the N6 atom of adenine; however, little is known about the mutagenicity of O6-dG-derived ICLs...
October 21, 2016: Chemical Research in Toxicology
Jiao Li, Yi Lu, Huiting Liang, Chunyan Tang, Lei Zhu, Jie Zhang, Renshi Xu
In order to searching the possible pathogenesis of amyotrophic lateral sclerosis (ALS), we examined the expression and distribution of FUS/TLS protein in the different anatomic regions, segments and neural cells of adult spinal cord at the different stages of the SOD1 wild-type and G93A transgenic mice using the fluorescent immunohistochemistry. Result revealed that, in the SOD1 wild-type mice, the FUS/TLS expression almost wasn't detected. However, in the SOD1 G93A mice, the FUS/TLS expression in the white matter was significantly more than that in the gray matter...
2016: International Journal of Biological Sciences
Zhoushuai Qin, Zhiqiang Bai, Ying Sun, Xiaohong Niu, Wei Xiao
In response to replication-blocking lesions, proliferating cell nuclear antigen (PCNA) can be sequentially ubiquitinated at the K164 residue leading to two modes of DNA-damage tolerance, namely translesion DNA synthesis (TLS) and error-free lesion bypass. Ectopic expression of PCNA fused with ubiquitin (Ub) lacking the two C-terminal Gly residues resembles PCNA monoubiquitination-mediated TLS. However, if the fused Ub contains C-terminal Gly residues, it is further polyubiquitinated and inhibits cell proliferation...
October 18, 2016: Cell Cycle
Catherine Sautès-Fridman, Myriam Lawand, Nicolas A Giraldo, Hélène Kaplon, Claire Germain, Wolf Herman Fridman, Marie-Caroline Dieu-Nosjean
Tertiary lymphoid structures (TLS) are ectopic lymphoid aggregates that reflect lymphoid neogenesis occurring in tissues at sites of inflammation. They are detected in tumors where they orchestrate local and systemic anti-tumor responses. A correlation has been found between high densities of TLS and prolonged patient's survival in more than 10 different types of cancer. TLS can be regulated by the same set of chemokines and cytokines that orchestrate lymphoid organogenesis and by regulatory T cells. Thus, TLS offer a series of putative new targets that could be used to develop therapies aiming to increase the anti-tumor immune response...
2016: Frontiers in Immunology
Seiya Urae, Kayori Tsuruoka, Sayaka Kuroya, Yugo Shibagaki
Tumor lysis syndrome (TLS) is a metabolic disorder that is generally associated with a malignancy leading to hyperuricemia, hyperphosphatemia, and acute kidney injury. On the other hand, we sometimes encounter these phenomena in nonmalignant disease, which has been referred to as tumor lysis-like syndrome in some studies. We herein experienced a case in which tumor lysis-like syndrome occurred in the course of therapy for eosinophilic disease of the lung, a nonmalignant disease. Even in nonmalignant disease, massive cell lysis induced by therapy can cause phenomena such as TLS or tumor lysis-like syndrome...
2016: Internal Medicine
Yoshihiro Kino, Chika Washizu, Masaru Kurosawa, Mizuki Yamada, Hiroshi Doi, Toru Takumi, Hiroaki Adachi, Masahisa Katsuno, Gen Sobue, Geoffrey G Hicks, Nobutaka Hattori, Tomomi Shimogori, Nobuyuki Nukina
FUS/TLS is an RNA/DNA-binding protein associated with neurodegenerative diseases including amyotrophic lateral sclerosis and frontotemporal lobar degeneration. Previously, we found that a prion-like domain in the N-terminus of FUS/TLS mediates co-aggregation between FUS/TLS and mutant huntingtin, the gene product of Huntington's disease (HD). Here, we show that heterozygous knockout of FUS/TLS worsened the phenotypes of model mice of (HD, but not spinal and bulbar muscular atrophy (SBMA). This difference was correlated with the degree of pathological association between disease proteins and FUS/TLS...
October 14, 2016: Scientific Reports
Qiang Niu, Xu Yang, Shouwan Gao, Pengpeng Chen, Shibing Chan
Localization is crucial for the monitoring applications of cities, such as road monitoring, environment surveillance, vehicle tracking, etc. In urban road sensor networks, sensors are often sparely deployed due to the hardware cost. Under this sparse deployment, sensors cannot communicate with each other via ranging hardware or one-hop connectivity, rendering the existing localization solutions ineffective. To address this issue, this paper proposes a novel Traffic Lights Schedule-based localization algorithm (TLS), which is built on the fact that vehicles move through the intersection with a known traffic light schedule...
October 10, 2016: Sensors
M Grande, H Murua, I Zudaire, E J Arsenault-Pernet, F Pernet, N Bodin
The lipid composition of somatic and reproductive tissues was determined for female skipjack tuna Katsuwonus pelamis caught in the western Indian Ocean between latitude 10° N and 20° S and longitude 40° and 70° E. The highest total lipid (TL) contents were in the liver and gonads, with white muscle levels approximately three-fold lower. Three lipid classes dominated: triacylglycerols (TAG), sterol esters and wax esters (SE-WE) and phospholipids (PL). Collectively, these accounted for between 70 and 80% of TLs...
October 11, 2016: Journal of Fish Biology
Du Shiyao, Bin Ni, Xuhua Lu, Ning Xie, Xiang Guo, Qunfeng Guo, Jun Yang, Fei Chen
OBJECTIVE: To analyze the clinical outcomes of our experience with unilateral C2 translaminar screw technique and evaluate its feasibility as an alternative or salvage of the pedicle screw. METHODS: Eleven consecutive adult patients suffering from atlantoaxial instability who underwent hybrid fixation techniques using unilateral translaminar screw (TLS) combined with contralateral C2 pedicle screw (PS) and bilateral C1 lateral mass screw via posterior arch (LMS) from January 2010 to December 2013 were retrospectively investigated...
October 4, 2016: World Neurosurgery
Erika N Guerrero, Haibo Wang, Joy Mitra, Pavana M Hegde, Sara E Stowell, Nicole F Liachko, Brian C Kraemer, Ralph M Garruto, K S Rao, Muralidhar L Hegde
Amyotrophic lateral sclerosis (ALS), a common motor neuron disease affecting two per 100,000 people worldwide, encompasses at least five distinct pathological subtypes, including, ALS-SOD1, ALS-C9orf72, ALS-TDP-43, ALS-FUS and Guam-ALS. The etiology of a major subset of ALS involves toxicity of the TAR DNA-binding protein-43 (TDP-43). A second RNA/DNA binding protein, fused in sarcoma/translocated in liposarcoma (FUS/TLS) has been subsequently associated with about 1% of ALS patients. While mutations in TDP-43 and FUS have been linked to ALS, the key contributing molecular mechanism(s) leading to cell death are still unclear...
September 28, 2016: Progress in Neurobiology
Amandine Chenet, Alexandra Gosseaume, Sandrine Wiertlewski, Brigitte Perrouin-Verbe
OBJECTIVE: Cognitive impairment in multiple sclerosis (MS) is common, present in 40 to 60% of patients, and is under diagnosis. Currently, there is no effective drug and non-pharmacological treatments that are developed. We propose to evaluate the effectiveness of exercise training (ET) on cognitive impairment. MATERIALS/PATIENTS AND METHODS: The study included patients from University Hospital of Nantes with an EDSS of between 3 and 4.5. They followed an exercise therapy program during 8 weeks, in groups, including aerobic physical activity (Nordic walking, gymnastics and exercise of balance) and recreational activities (billiards and boccia)...
September 2016: Annals of Physical and Rehabilitation Medicine
Marc Genty, Richard Devy, Gilles Edan, Philippe Lehert
OBJECTIVE: Multiple sclerosis (MS) severely affect quality of life (QoL). Pharmacological treatments demonstrated benefits on clinical endpoints, however, without proving a significant effect on QoL, we test the effect of a cognitive behavioural therapy (CBT) on QoL and investigate the association between QoL and illness progression. MATERIALS/PATIENTS AND METHODS: One-year multicenter controlled multivariate-matched study on relapsing remitting ms (RRMS) patient expended disability status scale (EDSS) <4 ms duration<2 years treated by interferon in 11 French centres...
September 2016: Annals of Physical and Rehabilitation Medicine
K Karzis, M Kalogeris, D Mandalidis, N Geladas, K Karteroliotis, S Athanasopoulos
The purpose of this study was to investigate Achilles tendon blood flow in individuals with overpronated feet during non-weight- and weight-bearing positions. Achilles tendon blood flow was measured by means of the pulsatility index (PI) and the resistance index (RI) in 15 male individuals with overpronated feet and 15 counterparts with normal feet, using power Doppler ultrasonography (PDI). Achilles tendon ultrasonographic (US) assessment was performed at its musculo-tendinous junction (MTJ), mid-tendon (MT), and osseotendinous junction (OTJ) at a non-weight-bearing relaxed position (RP) and during two-leg stance (TLS) and one-leg upright stance (OLS)...
September 27, 2016: Scandinavian Journal of Medicine & Science in Sports
Lianwen Zhang, Michael B Steele, Nathan Jenks, Jacquelyn Grell, Marshall Behrens, Rebecca Nace, Shruthi Naik, Mark J Federspiel, Stephen J Russell, Kah-Whye Peng
Tumor selective oncolytic vesicular stomatitis viruses (VSVs) are being evaluated in clinical trials. Here we report that the MPC-11 murine plasmacytoma model is so extraordinarily susceptible to oncolytic VSVs that a low dose of virus leads to extensive intratumoral viral replication, sustained viremia, intravascular coagulation and a rapidly fatal tumor lysis syndrome (TLS). Rapid softening, shrinkage and hemorrhagic necrosis of flank tumors was noted within 1-2 days after virus administration, leading to hyperkalemia, hyperphosphatemia, hypocalcemia, hyperuricemia, increase in plasma cell free DNA, lymphopenia, consumptive coagulopathy, increase in fibrinogen degradation products, decreased liver function tests, dehydration, weight loss, and euthanasia or death after 5-8 days...
September 27, 2016: Molecular Therapy: the Journal of the American Society of Gene Therapy
Guolong Li, Zhongzhou Ren, Xin Zhang
We demonstrate that the phonon elastic scattering leads to a dominant dissipation in crystals at low temperature. The two-level systems (TLSs) should be responsible for the elastic scattering, whereas the dissipation induced by static-point defects (SPDs) can not be neglected. One purpose of this work is to show how the energy splitting distribution of the TLS ensemble affects the dissipation. Besides, this article displays the proportion of phonon-TLS elastic scattering to total phonon dissipation. The coupling coefficient of phonon-SPD scattering and the constant P0 of the TLS distribution are important that we estimate their magnitudes in this paper...
September 26, 2016: Scientific Reports
Akira Sassa, Melike Ҫağlayan, Yesenia Rodriguez, William A Beard, Samuel H Wilson, Takehiko Nohmi, Masamitsu Honma, Manabu Yasui
Numerous ribonucleotides are incorporated into the genome during DNA replication. Oxidized ribonucleotides can also be erroneously incorporated into DNA. Embedded ribonucleotides destabilize the structure of DNA and retard DNA synthesis by DNA polymerases (pols), leading to genomic instability. Mammalian cells possess translesion DNA synthesis (TLS) pols that bypass DNA damage. The mechanism of TLS and repair of oxidized ribonucleotides remains to be elucidated. To address this, we analyzed the miscoding properties of the ribonucleotides riboguanosine (rG) and 7,8-dihydro-8-oxo-riboguanosine (8-oxo-rG) during TLS catalyzed by the human TLS pols κ and η in vitro...
September 22, 2016: Journal of Biological Chemistry
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