keyword
https://read.qxmd.com/read/38371733/antimicrobial-peptide-poly-ethylene-glycol-conjugates-connecting-molecular-architecture-solution-properties-and-functional-performance
#21
JOURNAL ARTICLE
Zixian Cui, Matthew A Crawford, Blake A Rumble, Megan M Krogh, Molly A Hughes, Rachel A Letteri
Antimicrobial peptides (AMPs) are promising alternatives to conventional antibiotics for treating infections caused by drug-resistant bacteria; yet, many peptides are limited by toxicity to eukaryotic cells and instability in biological environments. Conjugation to linear polymers that reduce cytotoxicity and improve stability, however, often decreases antimicrobial activity. In this work, we combine the biocompatibility advantages of poly(ethylene glycol) (PEG) with the efficacy merits of nonlinear polymer architectures that accommodate multiple AMPs per molecule...
February 14, 2024: ACS Polym Au
https://read.qxmd.com/read/38354953/studies-on-the-in-vitro-mechanism-and-in-vivo-therapeutic-effect-of-the-antimicrobial-peptide-acp5-against-trichophyton-mentagrophytes
#22
JOURNAL ARTICLE
Kuiming Zou, Shaojie Zhang, Kedong Yin, Shiming Ren, Mengjun Zhang, Xiatong Li, Lixin Fan, Ruiling Zhang, Ruifang Li
Trichophyton mentagrophytes is a zoophilic dermatophyte that can cause dermatophytosis in humans and animals. Antimicrobial peptides (AMPs) are considered as a promising agent to overcome the drug-resistance of T. mentagrophytes. Our findings suggest that cationic antimicrobial peptide (ACP5) not only possesses stronger activity against T. mentagrophytes than fluconazole, but also shows lower toxicity to L929 mouse fibroblast cells than terbinafine. Notably, its resistance development rate after resistance induction was lower than terbinafine...
February 12, 2024: Peptides
https://read.qxmd.com/read/38337177/development-of-a-novel-hybrid-antimicrobial-peptide-for-enhancing-antimicrobial-spectrum-and-potency-against-food-borne-pathogens
#23
JOURNAL ARTICLE
Jinzhi Han, Peifen Wu, Jie Yang, Yanlin Weng, Yayi Lin, Zhiying Chen, Fengfan Yu, Xucong Lü, Li Ni
AIMS: To address the increasingly serious challenge of the transmission of foodbrone pathogens in the food chain. METHODS AND RESULTS: In this study, we employed rational design strategies, including truncation, amino acid substitution, and heterozygosity, to generate seven engineered peptides with α-helical structure, cationic property, and amphipathic characteristics based on the original Abhisin template. Among them, as the hybird AMP, AM exhibits exceptional stability, minimal toxicity, as well as broad-spectrum and potent antimicrobial activity against foodborne pathogens...
February 9, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38329629/a-comprehensive-investigation-of-the-medicinal-efficacy-of-antimicrobial-fusion-peptides-expressed-in-probiotic-bacteria-for-the-treatment-of-pan-drug-resistant-pdr-infections
#24
JOURNAL ARTICLE
Maryam Safarpour-Dehkordi, Omid Chabok, Mohsen Asgari, Reyhaneh Khademi, Abbas Doosti
The present work aimed to examine the intracellular antibacterial efficacy of Recombinant Lactobacillus acidophilus/antimicrobial peptides (AMPs) Melittin and Alyteserin-1a, specifically targeting Gram-negative bacteria. The first assessment was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Recombinant L. acidophilus/AMPs versus Gram-negative and Gram-positive bacteria. In addition, the researchers examined the in vitro viability and safety of AMPs generated by L...
February 8, 2024: Archives of Microbiology
https://read.qxmd.com/read/38319435/antimicrobial-and-antitumor-properties-of-anuran-peptide-temporin-shf-induce-apoptosis-in-a549-lung-cancer-cells
#25
JOURNAL ARTICLE
Anet Antony, Anupama Kizhakke Purayil, Shilpa Olakkaran, Shweta Dhannura, Shamasoddin Shekh, Konkallu Hanumae Gowd, Hunasanahally Puttaswamygowda Gurushankara
Temporin-SHf is a linear, ultra-short, hydrophobic, α-helix, and phe-rich cationic antimicrobial peptide. The antitumor activities and mechanism of temporin-SHf-induced cancer cell death are unknown. The temporin-SHf was synthesized by solid-phase Fmoc chemistry and antimicrobial and antitumor activities were investigated. Temporin-SHf was microbiocidal, non-hemolytic, and cytotoxic to human cancer cells but not to non-tumorigenic cells. It affected the cancer cells' lysosomal integrity and caused cell membrane damage...
February 6, 2024: Amino Acids
https://read.qxmd.com/read/38318188/the-current-landscape-of-the-antimicrobial-peptide-melittin-and-its-therapeutic-potential
#26
REVIEW
Hai-Qian Zhang, Chengbiao Sun, Na Xu, Wensen Liu
Melittin, a main component of bee venom, is a cationic amphiphilic peptide with a linear α-helix structure. It has been reported that melittin can exert pharmacological effects, such as antitumor, antiviral and anti-inflammatory effects in vitro and in vivo . In particular, melittin may be beneficial for the treatment of diseases for which no specific clinical therapeutic agents exist. Melittin can effectively enhance the therapeutic properties of some first-line drugs. Elucidating the mechanism underlying melittin-mediated biological function can provide valuable insights for the application of melittin in disease intervention...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38306774/profiling-and-source-tracking-of-the-microbial-populations-and-resistome-present-in-fish-products
#27
JOURNAL ARTICLE
Xiran Li, Hongye Wang, Hisham Abdelrahman, Anita Kelly, Luke Roy, Luxin Wang
Microorganisms in processing environments significantly impact the quality and safety of food products and can serve as potential reservoirs for antibiotic-resistant genes, contributing to public health concerns about antimicrobial resistance (AMR). Fish processing plants represent an understudied environment for microbiome mapping. This study investigated the microbial composition, prevalence of Listeria spp., and resistome structures in three catfish processing facilities in the southeastern United States...
January 25, 2024: International Journal of Food Microbiology
https://read.qxmd.com/read/38306481/viral-afterlife-sars-cov-2-as-a-reservoir-of-immunomimetic-peptides-that-reassemble-into-proinflammatory-supramolecular-complexes
#28
JOURNAL ARTICLE
Yue Zhang, Vanthana Bharathi, Tatsuya Dokoshi, Jaime de Anda, Lauryn Tumey Ursery, Nikhil N Kulkarni, Yoshiyuki Nakamura, Jonathan Chen, Elizabeth W C Luo, Lamei Wang, Hua Xu, Alison Coady, Raymond Zurich, Michelle W Lee, Tsutomu Matsui, HongKyu Lee, Liana C Chan, Athena A Schepmoes, Mary S Lipton, Rui Zhao, Joshua N Adkins, Geremy C Clair, Lance R Thurlow, Jonathan C Schisler, Matthew C Wolfgang, Robert S Hagan, Michael R Yeaman, Thomas M Weiss, Xinhua Chen, Melody M H Li, Victor Nizet, Silvio Antoniak, Nigel Mackman, Richard L Gallo, Gerard C L Wong
It is unclear how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leads to the strong but ineffective inflammatory response that characterizes severe Coronavirus disease 2019 (COVID-19), with amplified immune activation in diverse cell types, including cells without angiotensin-converting enzyme 2 receptors necessary for infection. Proteolytic degradation of SARS-CoV-2 virions is a milestone in host viral clearance, but the impact of remnant viral peptide fragments from high viral loads is not known...
February 6, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38305138/knockout-of-adenylosuccinate-synthase-pura-increases-susceptibility-to-colistin-in-escherichia-coli
#29
JOURNAL ARTICLE
Tomonori Kano, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito
Colistin is a cationic cyclic antimicrobial peptide used as a last resort against multidrug-resistant gram-negative bacteria. To understand the factors involved in colistin susceptibility, we screened colistin-sensitive mutants from an E. coli gene-knockout library (Keio collection). The knockout of purA, whose product catalyzes the synthesis of adenylosuccinate from IMP in the de novo purine synthesis pathway, resulted in increased sensitivity to colistin. Adenylosuccinate is subsequently converted to AMP, which is phosphorylated to produce ADP, a substrate for ATP synthesis...
February 1, 2024: FEMS Microbiology Letters
https://read.qxmd.com/read/38300464/easy-and-versatile-cellulosic-support-inhibiting-broad-spectrum-strains-synergy-between-photodynamic-antimicrobial-therapy-and-polymyxin-b
#30
JOURNAL ARTICLE
Florent Le Guern, Tan-Sothea Ouk, Phillipe Arnoux, Céline Frochot, Vincent Sol
Despite advances achieved in the health field over the last decade, infections caused by resistant bacterial strains are an increasingly important societal issue that needs to be addressed. New approaches have already been developed to overcome this problem. Photodynamic antimicrobial chemotherapy (PACT) could provide a promising alternative method to eradicate microbes. This approach has already inspired the development of innovative surfaces. Interesting results were achieved against Gram-positive bacteria, but it also appeared that Gram-negative strains, especially Pseudomonas aeruginosa, were less sensitive to PACT...
February 1, 2024: Photochemical & Photobiological Sciences
https://read.qxmd.com/read/38296556/rational-design-of-amphipathic-antimicrobial-peptides-with-alternating-l-d-amino-acids-that-form-helical-structures
#31
JOURNAL ARTICLE
Motoharu Hirano, Hidetomo Yokoo, Nobumichi Ohoka, Takahito Ito, Takashi Misawa, Makoto Oba, Takao Inoue, Yosuke Demizu
Antimicrobial peptides (AMPs) are promising therapeutic agents against bacteria. We have previously reported an amphipathic AMP Stripe composed of cationic L-Lys and hydrophobic L-Leu/L-Ala residues, and Stripe exhibited potent antimicrobial activity against Gram-positive and Gram-negative bacteria. Gramicidin A (GA), composed of repeating sequences of L- and D-amino acids, has a unique β6.3 -helix structure and exhibits broad antimicrobial activity. Inspired by the structural properties and antimicrobial activities of LD-alternating peptides such as GA, in this study, we designed Stripe derivatives with LD-alternating sequences...
2024: Chemical & Pharmaceutical Bulletin
https://read.qxmd.com/read/38295893/nmr-techniques-for-investigating-antimicrobial-peptides-in-model-membranes-and-bacterial-cells
#32
JOURNAL ARTICLE
Marc-Antoine Sani, Sunnia Rajput, David W Keizer, Frances Separovic
AMPs are short, mainly cationic membrane-active peptides found in all living organism. They perform diverse roles including signaling and acting as a line of defense against bacterial infections. AMPs have been extensively investigated as templates to facilitate the development of novel antimicrobial therapeutics. Understanding the interplay between these membrane-active peptides and the lipid membranes is considered to be a significant step in elucidating the specific mechanism of action of AMPs against prokaryotic and eukaryotic cells to aid the development of new therapeutics...
January 29, 2024: Methods: a Companion to Methods in Enzymology
https://read.qxmd.com/read/38294842/mechanism-of-protease-resistance-of-d-amino-acid-residue-containing-cationic-antimicrobial-heptapeptides
#33
JOURNAL ARTICLE
Tanumoy Sarkar, Suvankar Ghosh, Pradeep Kumar Sundaravadivelu, Gopal Pandit, Swapna Debnath, Rajkumar P Thummer, Priyadarshi Satpati, Sunanda Chatterjee
Antimicrobial peptides (AMPs) have been an alternate promising class of therapeutics in combating global antibiotic resistance threat. However, the short half-life of AMPs, owing to protease degradability, is one of the major bottlenecks in its commercial success. In this study, we have developed all-D-amino acid containing small cationic peptides P4C and P5C, which are completely protease-resistant, noncytotoxic, nonhemolytic, and potent against the ESKAPE pathogens in comparison to their L analogues. MD simulations suggested marginal improvement in the peptide-binding affinity to the membrane-mimetic SDS micelle (∼ 1 kcal/mol) in response to L → D conversion, corroborating the marginal improvement in the antimicrobial activity...
January 31, 2024: ACS Infectious Diseases
https://read.qxmd.com/read/38289163/chickpea-derived-modified-antimicrobial-peptides-kta-and-ktr-inactivate-staphylococcus-aureus-via-disrupting-cell-membrane-and-interfering-with-peptidoglycan-synthesis
#34
JOURNAL ARTICLE
Xinhui Zhang, Peipei Ma, Balarabe B Ismail, Zhehao Yang, Zhipeng Zou, Yujuan Suo, Xingqian Ye, Donghong Liu, Mingming Guo
The widespread bacterial contamination caused by foodborne pathogens has continuously driven the development of advanced and potent food antimicrobial agents. In this study, two novel antimicrobial peptides (AMPs) named KTA and KTR were obtained by modifying a natural AMP, Leg2, from chickpea storage protein legumin hydrolysates. They were further predicted to be stable hydrophobic cationic AMPs of α-helical structure with no hemolytic toxicity by several online servers. Moreover, the AMPs exerted superior antibacterial activity against two representative Staphylococcus aureus strains thanks to the increased hydrophobicity and positive charge, with minimum inhibition concentration value (4...
January 30, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38284202/discovery-of-natural-bispecific-antibodies-is-psoriasis-induced-by-a-toxigenic-corynebacterium-simulans-and-maintained-by-cidamps-as-autoantigens
#35
JOURNAL ARTICLE
Jens-Michael Schröder
The high abundance of Corynebacterium simulans in psoriasis skin suggests a contribution to the psoriasis aetiology. This hypothesis was tested in an exploratory study, where western blot (WB) analyses with extracts of heat-treated C. simulans and psoriasis serum-derived IgG exhibited a single 16 kDa-WB-band. Proteomic analyses revealed ribosomal proteins as candidate C. s.-antigens. A peptidomic analysis unexpectedly showed that psoriasis serum-derived IgG already contained 31 immunopeptides of Corynebacteria ssp...
January 2024: Experimental Dermatology
https://read.qxmd.com/read/38278338/antibacterial-activity-and-modes-of-action-of-a-novel-hepcidin-isoform-from-the-shrimp-scad-alepes-djedaba-forssk%C3%A3-l-1775
#36
JOURNAL ARTICLE
V V Anooja, K Archana, P P Athira, M V Anju, S Neelima, S Muhammed Musthafa, M Dhaneesha, T P Sajeevan, I S Bright Singh, Rosamma Philip
Hepcidin, initially identified in human blood ultrafiltrate as cysteine rich Liver Expressed Antimicrobial Peptide (LEAP-1), is a core molecular conduit between iron trafficking and immune response. Though a great share of studies has been focused on the iron regulatory function of hepcidins, investigations on the antimicrobial aspects are relatively less. The present study is aimed at identification of hepcidin from a teleost fish, Alepes djedaba followed by its recombinant expression, testing antibacterial property, stability and evaluation of cytotoxicity...
January 24, 2024: Fish & Shellfish Immunology
https://read.qxmd.com/read/38276499/lc-amp-f1-derived-from-the-venom-of-the-wolf-spider-lycosa-coelestis-exhibits-antimicrobial-and-antibiofilm-activities
#37
JOURNAL ARTICLE
Yuxin Song, Junyao Wang, Xi Liu, Shengwei Yu, Xing Tang, Huaxin Tan
In recent years, there has been a growing interest in antimicrobial peptides as innovative antimicrobial agents for combating drug-resistant bacterial infections, particularly in the fields of biofilm control and eradication. In the present study, a novel cationic antimicrobial peptide, named LC-AMP-F1, was derived from the cDNA library of the Lycosa coelestis venom gland. The sequence, physicochemical properties and secondary structure of LC-AMP-F1 were predicted and studied. LC-AMP-F1 was tested for stability, cytotoxicity, drug resistance, antibacterial activity, and antibiofilm activity in vitro compared with melittin, a well-studied antimicrobial peptide...
January 19, 2024: Pharmaceutics
https://read.qxmd.com/read/38275316/short-antimicrobial-peptide-derived-from-the-venom-gland-transcriptome-of-pamphobeteus-verdolaga-increases-gentamicin-susceptibility-of-multidrug-resistant-klebsiella-pneumoniae
#38
JOURNAL ARTICLE
Cristian Salinas-Restrepo, Ana María Naranjo-Duran, Juan Quintana, Julio Bueno, Fanny Guzman, Lina M Hoyos Palacio, Cesar Segura
Infectious diseases account for nine percent of annual human deaths, and the widespread emergence of antimicrobial resistances threatens to significantly increase this number in the coming decades. The prospect of antimicrobial peptides (AMPs) derived from venomous animals presents an interesting alternative for developing novel active pharmaceutical ingredients (APIs). Small, cationic and amphiphilic peptides were predicted from the venom gland transcriptome of Pamphobeteus verdolaga using a custom database of the arthropod's AMPs...
December 20, 2023: Antibiotics
https://read.qxmd.com/read/38275314/progressive-design-of-a-ranatuerin-2-peptide-from-amolops-wuyiensis-enhancement-of-bioactivity-and-in-vivo-efficacy
#39
JOURNAL ARTICLE
Aifang Yao, Tianxing Liu, Yuhai Cai, Siqi Zhou, Xiaoling Chen, Mei Zhou, Chengbang Ma, Tianbao Chen, Chris Shaw, Lei Wang
Antimicrobial peptides (AMPs) that exert multiple functions are considered promising candidates to combat the bacterial drug resistance crisis. Nowadays, targeted peptide modification has been widely recognised to improve biological activity and make up for deficiencies in clinical applications such as toxicity. In this study, a helix-loop peptide was isolated and identified from the skin secretion of the Wuyi torrent frog Amolops wuyiensis , namely, ranatuerin-2-AW (R2AW) (GFMDTAKNVAKNVAATLLDKLKCKITGGC). Target modifications were made to R2AW to study the structure-activity relationships and to optimise its bioactivities...
December 19, 2023: Antibiotics
https://read.qxmd.com/read/38271799/all-hydrocarbon-stapling-enables-improvement-of-antimicrobial-activity-and-proteolytic-stability-of-peptide-figainin-2
#40
JOURNAL ARTICLE
Jingwen Xue, Yinxue Fu, Huang Li, Ting Zhang, Wei Cong, Honggang Hu, Zhiyuan Lu, Fang Yan, Yulei Li
Figainin 2 is a cationic, hydrophobic, α-helical host-defense peptide with 28 residues, which was isolated from the skin secretions of the Chaco tree frog. It shows potent inhibitory activity against both Gram-negative and Gram-positive pathogens and has garnered considerable interest in developing novel classes of natural antibacterial agents. However, as a linear peptide, conformational flexibility and poor proteolytic stability hindered its development as antibacterial agent. To alleviate its susceptibility to proteolytic degradation and improve its antibacterial activity, a series of hydrocarbon-stable analogs of Figainin 2 were synthesized and evaluated for their secondary structure, protease stability, antimicrobial, and hemolytic activities...
January 25, 2024: Journal of Peptide Science
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