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cationic antimicrobial peptide

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https://www.readbyqxmd.com/read/28938518/antimicrobial-peptides-amps-the-quintessential-offense-and-defense-molecules-are-more-than-antimicrobials
#1
REVIEW
Seema Patel, Nadeem Akhtar
Antimicrobial peptides (AMPs) are cationic amphiphilic molecules with α-helix or β-sheet linear motifs and linear or cyclic configurations. For their role in 'defense and offense', they are present in all living organisms. AMPs are named so, as they inhibit a wide array of microbes by membrane pore formation and subsequent perturbation of mitochondrial membrane ionic balance. However, their functional repertoire is expanding with validated roles in cytotoxicity, wound healing, angiogenesis, apoptosis, and chemotaxis [1]...
September 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28931578/lipid-topology-and-electrostatic-interactions-underpin-lytic-activity-of-linear-cationic-antimicrobial-peptides-in-membranes
#2
David J Paterson, Manlio Tassieri, Julien Reboud, Rab Wilson, Jonathan M Cooper
Linear cationic antimicrobial peptides are a diverse class of molecules that interact with a wide range of cell membranes. Many of these peptides disrupt cell integrity by forming membrane-spanning pores that ultimately lead to their death. Despite these peptides high potency and ability to evade acquired bacterial drug resistance, there is a lack of knowledge on their selectivity and activity mechanisms. Such an understanding would provide an informative framework for rational design and could lead to potential antimicrobial therapeutic targets...
September 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28931287/enhanced-capture-of-bacteria-and-endotoxin-by-antimicrobial-wlbu2-peptide-tethered-on-polyethylene-oxide-spacers
#3
Ramya Raman, Miranda A Raper, Erik Hahn, Kate F Schilke
Severe sepsis is a life threatening immune response that may be caused by endotoxins (lipopolysaccharides) in circulating bacterial cell wall fragments. Hemoperfusion through a sorbent column coated with the antimicrobial peptide polymyxin B (PMB) is a promising treatment for sepsis. However, PMB is cytotoxic and neurotoxic, and is a membrane disruptor that may fragment endotoxin vesicles. In addition, the blood is not protected from nonspecific interactions with the synthetic surface of the solid support. These effects may be responsible for the variety of undesirable clinical outcomes, including nonspecific adsorption of proteins, blood cell damage, platelet activation, and a lack of clear evidence of efficacy of the current hemoperfusion products...
September 20, 2017: Biointerphases
https://www.readbyqxmd.com/read/28926942/the-semi-synthetic-peptide-lin-sb056-1-in-combination-with-edta-exerts-strong-antimicrobial-and-antibiofilm-activity-against-pseudomonas-aeruginosa-in-conditions-mimicking-cystic-fibrosis-sputum
#4
Giuseppantonio Maisetta, Lucia Grassi, Semih Esin, Ilaria Serra, Mariano A Scorciapino, Andrea C Rinaldi, Giovanna Batoni
Pseudomonas aeruginosa is a major cause of chronic lung infections in cystic fibrosis (CF) patients. The ability of the bacterium to form biofilms and the presence of a thick and stagnant mucus in the airways of CF patients largely contribute to antibiotic therapy failure and demand for new antimicrobial agents able to act in the CF environment. The present study investigated the anti-P. aeruginosa activity of lin-SB056-1, a recently described semi-synthetic antimicrobial peptide, used alone and in combination with the cation chelator ethylenediaminetetraacetic acid (EDTA)...
September 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28925099/highly-efficiency-antibacterial-diblock-copolypeptides-based-on-lysine-and-phenylalanine
#5
Xiaokai Su, Xinyu Zhou, Zhengzhong Tan, Chuncai Zhou
A series of amphiphilic diblock copolypeptides (K30 -b-F15 , K30 -b-F30 , and K30 -b-F45 ) were synthesized via N-carboxy-α-amino-anhydride ring-opening polymerization. The copolypeptides had excellent antibacterial efficacy to both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. The minimum inhibitory concentrations (MICs) against E. coli and S. aureus are 8 μg mL(-1) and 2 μg mL(-1) , respectively, lower than most natural and artificial antimicrobial peptides (AMPs). The morphological changes of the bacteria treated with diblock copolypeptides were investigated by transmission electron microscopy; the results proved that the diblock copolypeptides had a similar antibacterial pore-forming mechanism to natural cationic peptides...
September 19, 2017: Biopolymers
https://www.readbyqxmd.com/read/28921990/disruption-of-asymmetric-lipid-bilayer-models-mimicking-the-outer-membrane-of-gram-negative-bacteria-by-an-active-plasticin
#6
Jean-Philippe Michel, Yingxiong Wang, Irena Kiesel, Yuri Gerelli, Veronique Rosilio
The outer membrane (OM) of Gram-negative bacteria is a complex and asymmetric bilayer that antimicrobial peptides must disrupt in order to provoke the cell lysis. The inner and external leaflets of the OM are mainly composed of phospholipids (PL), and lipopolysaccharide (LPS), respectively. Supported lipid bilayers are interesting model systems to mimic the lipid asymmetric scaffold of the OM and determine the quantitative and mechanistic effect of antimicrobial agents, using complementary physicochemical techniques...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28921048/antibacterial-and-antitumor-activity-of-bogorol-b-jx-isolated-from-brevibacillus-laterosporus-jx-5
#7
Hongxia Jiang, Chao Ji, Junkang Sui, Rongbo Sa, Xiaohui Wang, Xunli Liu, Tai L Guo
Antimicrobial peptides are promising anti-infective agent candidates because they have a broad antimicrobial spectrum and bioactivity and are unlikely to elicit antibiotic resistance. The bogorols represent a new cationic antibiotic peptide and possess great therapeutic potential because of their bioactivity and precise mode of action. Here, we report that Bogorol B-JX (BBJX), a peptide previously isolated from Brevibacillus laterosporus JX-5 by us, has significant antibacterial and antitumor activities in vitro...
September 18, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28906060/novel-coordination-of-lipopolysaccharide-modifications-in-vibrio-cholerae-promotes-camp-resistance
#8
Carmen M Herrera, Jeremy C Henderson, Alexander A Crofts, M Stephen Trent
In the environment and during infection, the human intestinal pathogen Vibrio cholerae must overcome noxious compounds that damage the bacterial outer membrane. The El Tor and classical biotypes of O1 V. cholerae show striking differences in their resistance to membrane disrupting cationic antimicrobial peptides (CAMPs), such as polymyxins. The classical biotype is susceptible to CAMPs, but current pandemic El Tor biotype isolates gain CAMP resistance by altering the net charge of their cell surface through glycine modification of lipid A...
September 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28888370/biophysical-and-biological-properties-of-small-linear-peptides-derived-from-crotamine-a-cationic-antimicrobial-antitumoral-toxin-with-cell-penetrating-and-cargo-delivery-abilities
#9
C Dal Mas, D A Pinheiro, J D Campeiro, B Mattei, V Oliveira, E B Oliveira, A Miranda, K R Perez, M A F Hayashi
Crotamine is a natural polypeptide from snake venom which delivers nucleic acid molecules into cells, besides having pronounced affinity for negatively charged membranes and antifungal activity. We previously demonstrated that crotamine derived short linear peptides were not very effective as antifungal, although the non-structured recombinant crotamine was overridingly more potent compared to the native structured crotamine. Aiming to identify the features necessary for the antifungal activity of crotamine, two linear short peptides, each comprising half of the total positively charged amino acid residues of the full-length crotamine were evaluated here to show that these linear peptides keep the ability to interact with lipid membrane model systems with different phospholipid compositions, even after forming complexes with DNA...
September 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28874470/inhibition-of-the-atp-synthase-eliminates-the-intrinsic-resistance-of-staphylococcus-aureus-towards-polymyxins
#10
Martin Vestergaard, Katrine Nøhr-Meldgaard, Martin Saxtorph Bojer, Christina Krogsgård Nielsen, Rikke Louise Meyer, Christoph Slavetinsky, Andreas Peschel, Hanne Ingmer
Staphylococcus aureus is intrinsically resistant to polymyxins (polymyxin B and colistin), an important class of cationic antimicrobial peptides used in treatment of Gram-negative bacterial infections. To understand the mechanisms underlying intrinsic polymyxin resistance in S. aureus, we screened the Nebraska Transposon Mutant Library established in S. aureus strain JE2 for increased susceptibility to polymyxin B. Nineteen mutants displayed at least 2-fold reductions in MIC, while the greatest reductions (8-fold) were observed for mutants with inactivation of either graS, graR, vraF, or vraG or the subunits of the ATP synthase (atpA, atpB, atpG, or atpH), which during respiration is the main source of energy...
September 5, 2017: MBio
https://www.readbyqxmd.com/read/28874446/characterization-of-two-novel-lipopolysaccharide-phosphoethanolamine-transferases-in-pasteurella-multocida-and-their-role-in-resistance-to-cathelicidin-2
#11
Marina Harper, Amy Wright, Frank St Michael, Jianjun Li, Deanna Deveson Lucas, Mark Ford, Ben Adler, Andrew D Cox, John D Boyce
The lipopolysaccharide (LPS) produced by the Gram-negative bacterial pathogen Pasteurella multocida has phosphoethanolamine (PEtn) residues attached to lipid A, 3-deoxy-D-manno-octulosonic acid (Kdo), heptose and galactose. In this study, we show that PEtn is transferred to lipid A by the P. multocida EptA homologue, PetL, and is transferred to galactose by a novel PEtn transferase that is unique to P. multocida called PetG. Transcriptomic analyses indicated that petL expression was positively regulated by the global regulator Fis and negatively regulated by an Hfq-dependent small RNA...
September 5, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28858240/toxicity-effects-of-functionalized-quantum-dots-gold-and-polystyrene-nanoparticles-on-target-aquatic-biological-models-a-review
#12
REVIEW
Giovanni Libralato, Emilia Galdiero, Annarita Falanga, Rosa Carotenuto, Elisabetta de Alteriis, Marco Guida
Nano-based products are widespread in several sectors, including textiles, medical-products, cosmetics, paints and plastics. Nanosafety and safe-by-design are driving nanoparticle (NP) production and applications through NP functionalization (@NPs). Indeed, @NPs frequently present biological effects that differ from the parent material. This paper reviews the impact of quantum dots (QDs), gold nanoparticles (AuNPs), and polystyrene-cored NPs (PSNPs), evidencing the role of NP functionalization in toxicity definition...
August 31, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28855917/peptidomic-analysis-of-the-venom-of-the-solitary-bee-xylocopa-appendiculata-circumvolans
#13
Kohei Kazuma, Kenji Ando, Ken-Ichi Nihei, Xiaoyu Wang, Marisa Rangel, Marcia Regina Franzolin, Kanami Mori-Yasumoto, Setsuko Sekita, Makoto Kadowaki, Motoyoshi Satake, Katsuhiro Konno
BACKGROUND: Among the hymenopteran insect venoms, those from social wasps and bees - such as honeybee, hornets and paper wasps - have been well documented. Their venoms are composed of a number of peptides and proteins and used for defending their nests and themselves from predators. In contrast, the venoms of solitary wasps and bees have not been the object of further research. In case of solitary bees, only major peptide components in a few venoms have been addressed. Therefore, the aim of the present study was to explore the peptide component profile of the venom from the solitary bee Xylocopa appendiculata circumvolans by peptidomic analysis with using LC-MS...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28853858/enhanced-photobactericidal-and-targeting-properties-of-a-cationic-porphyrin-following-the-attachment-of-polymyxin-b
#14
Florent Le Guern, Vincent Sol, Catherine Ouk, Philippe Arnoux, Céline Frochot, Tan-Sothea Ouk
A novel compound consisting of a cationic porphyrin covalently attached to a derivative of polymyxin B has been synthesized and presents enhanced activity and targeting properties compared to the usual cationic porphyrins recognized as efficient photosensitizers in photodynamic antimicrobial chemotherapy (PACT). A synthesis pathway was established to preserve the bactericidal activity of the peptide. Accordingly, the N-terminal amino acid (l-2,4-diaminobutyric acid) of polymyxin B (PMB) was switched for a cysteine residue...
September 11, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28844739/recent-progress-on-the-application-of-2-h-solid-state-nmr-to-probe-the-interaction-of-antimicrobial-peptides-with-intact-bacteria
#15
REVIEW
Valerie Booth, Dror E Warschawski, Nury P Santisteban, Marwa Laadhari, Isabelle Marcotte
Discoveries relating to innate immunity and antimicrobial peptides (AMPs) granted Bruce Beutler and Jules Hoffmann a Nobel prize in medicine in 2011, and opened up new avenues for the development of therapies against infections, and even cancers. The mechanisms by which AMPs interact with, and ultimately disrupt, bacterial cell membranes is still, to a large extent, incompletely understood. Up until recently, this mechanism was studied using model lipid membranes that failed to reproduce the complexity of molecular interactions present in real cells comprising lipids but also membrane proteins, a cell wall containing peptidoglycan or lipopolysaccharides, and other molecules...
August 24, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28844103/the-mla-pathway-is-critical-for-pseudomonas-aeruginosa-resistance-to-outer-membrane-permeabilization-and-host-innate-immune-clearance
#16
Jason Munguia, Doris L LaRock, Hannah Tsunemoto, Joshua Olson, Ingrid Cornax, Joseph Pogliano, Victor Nizet
Pseudomonas aeruginosa is an important opportunistic pathogen that has become a serious problem due to increased rates of antibiotic resistance. Due to this along with a dearth in novel antibiotic development, especially against Gram-negative pathogens, new therapeutic strategies are needed to prevent a post-antibiotic era. Here, we describe the importance of the vacJ/Mla pathway in resisting bactericidal actions of the host innate immune response. P. aeruginosa tn5 transposon mutants in genes from the VacJ/Mla pathway showed increased susceptibility to killing by the host cathelicidin antimicrobial peptide, LL-37, when compared to the wild-type parent strain...
August 26, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28834198/potent-antimicrobial-activity-of-lipidated-short-%C3%AE-%C3%AE-hybrid-peptides
#17
Sushil N Benke, Hirekodathakallu V Thulasiram, Hosahudya N Gopi
Herein we report the potent antimicrobial activity of α,γ-hybrid lipopeptides composed of 1:1 alternating α- and γ-amino acids. Along with their potent antimicrobial activity against various Gram-positive and Gram-negative bacteria, these hybrid lipopeptides were found to be less hemolytic. Studies into the mechanism of action revealed that these short cationic lipopeptides bind and disrupt the bacterial cell membrane. Time-kill kinetics analyses revealed that the potent α,γ-hybrid lipopeptides completely inhibit bacterial growth in less than 20 minutes...
August 18, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28833783/intramolecular-cyclization-of-the-antimicrobial-peptide-polybia-mpi-with-triazole-stapling-influence-on-stability-and-bioactivity
#18
Beijun Liu, Wei Zhang, Sanhu Gou, Haifeng Huang, Jia Yao, Zhibin Yang, Hui Liu, Chao Zhong, Beiyin Liu, Jingman Ni, Rui Wang
Cationic antimicrobial peptides have attracted increasing attention as a novel class of antibiotics to treat infectious diseases caused by pathogenic bacteria. However, susceptibility to protease is a shortcoming in their development. Cyclization is one approach to increase the proteolytic resistance of peptides. Therefore, to improve the proteolytic resistance of Polybia-MPI, we have synthesized the MPI cyclic analogs C-MPI-1 (i-to-i+4) and C-MPI-2 (i-to-i+6) by copper(I)-catalyzed azide-alkyne cycloaddition...
August 23, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28829401/myticalins-a-novel-multigenic-family-of-linear-cationic-antimicrobial-peptides-from-marine-mussels-mytilus-spp
#19
Gabriele Leoni, Andrea De Poli, Mario Mardirossian, Stefano Gambato, Fiorella Florian, Paola Venier, Daniel N Wilson, Alessandro Tossi, Alberto Pallavicini, Marco Gerdol
The application of high-throughput sequencing technologies to non-model organisms has brought new opportunities for the identification of bioactive peptides from genomes and transcriptomes. From this point of view, marine invertebrates represent a potentially rich, yet largely unexplored resource for de novo discovery due to their adaptation to diverse challenging habitats. Bioinformatics analyses of available genomic and transcriptomic data allowed us to identify myticalins, a novel family of antimicrobial peptides (AMPs) from the mussel Mytilus galloprovincialis, and a similar family of AMPs from Modiolus spp...
August 22, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28827052/expression-and-characterization-of-the-antimicrobial-peptide-abp-dhc-cecropin-a-in-the-methylotrophic-yeast-pichia-pastoris
#20
Ming Sang, Hui Wei, Jiaxin Zhang, Zhiheng Wei, Xiaolong Wu, Yan Chen, Qiang Zhuge
ABP-dHC-cecropin A is a linear cationic peptide that exhibits antimicrobial properties. To explore a new approach for expression of ABP-dHC-cecropin A using the methylotrophic yeast Pichia pastoris, we cloned the ABP-dHC-cecropin A gene into the vector pPICZαA. The SacI-linearized plasmid pPICZαA-ABP-dHC-cecropin A was then transformed into P. pastoris GS115 by electroporation. Expression was induced after a 96-h incubation with 0.5% methanol at 20 °C in a culture supplied with 2% casamino acids to avoid proteolysis...
August 4, 2017: Protein Expression and Purification
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