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https://www.readbyqxmd.com/read/29332216/characteristics-of-arg-carrying-plasmidome-in-the-cultivable-microbial-community-from-wastewater-treatment-system-under-high-oxytetracycline-concentration
#1
Yanhong Shi, Hong Zhang, Zhe Tian, Min Yang, Yu Zhang
Studies on antibiotic production wastewater have shown that even a single antibiotic can select for multidrug resistant bacteria in aquatic environments. It is speculated that plasmids are an important mechanism of multidrug resistance (MDR) under high concentrations of antibiotics. Herein, two metagenomic libraries were constructed with plasmid DNA extracted from cultivable microbial communities in a biological wastewater treatment reactor supplemented with 0 (CONTROL) or 25 mg/L of oxytetracycline (OTC-25)...
January 13, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29331911/enhanced-photocatalytic-degradation-of-sulfamethoxazole-by-zinc-oxide-photocatalyst-in-the-presence-of-fluoride-ions-optimization-of-parameters-and-toxicological-evaluation
#2
Amir Mirzaei, Laleh Yerushalmi, Zhi Chen, Fariborz Haghighat, Jianbo Guo
The presence of antibiotics in water bodies has received increasing attention since they are continuously introduced and detected in the environment and may cause unpredictable environmental hazards and risks. The photocatalytic degradation of sulfamethoxazole (SMX) by ZnO in the presence of fluoride ions (F-ZnO) was evaluated. The effects of operating parameters on the efficiency of SMX removal were investigated by using response surface methodology (RSM). Under the optimum condition, i.e. photocatalyst dosage = 1...
January 11, 2018: Water Research
https://www.readbyqxmd.com/read/29330184/microbiomes-in-dishwashers-analysis-of-the-microbial-diversity-and-putative-opportunistic-pathogens-in-dishwasher-biofilm-communities
#3
Prem Krishnan Raghupathi, Jerneja Zupančič, Asker Daniel Brejnrod, Samuel Jacquiod, Kurt Houf, Mette Burmølle, Nina Gunde-Cimerman, Søren J Sørensen
Extreme habitats are not only limited to natural environments, but also apply to man-made systems, for instance household appliances such as dishwashers. Limiting factors, such as high temperatures, high and low pH, high NaCl concentrations, presence of detergents and shear force from water during washing cycles define the microbial survival in this extreme system. Fungal and bacterial diversity in biofilms isolated from rubber seals of 24 different household dishwashers were investigated using next generation sequencing...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29322334/detection-of-%C3%AE-lactamase-encoding-genes-in-feces-soil-and-water-from-a-brazilian-pig-farm
#4
João Pedro Rueda Furlan, Eliana Guedes Stehling
β-lactam antibiotics are widely used for the treatment of different types of infections worldwide and the resistance to these antibiotics has grown sharply, which is of great concern. Resistance to β-lactams in gram-negative bacteria is mainly due to the production of β-lactamases, which are classified according to their functional activities. The aim of this study was to verify the presence of β-lactamases encoding genes in feces, soil, and water from a Brazilian pig farm. Different β-lactamases encoding genes were found, including blaCTX-M-Gp1, blaCTX-M-Gp9, blaSHV, blaOXA-1-like, blaGES, and blaVEB...
January 10, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29321692/geobacteraceae-are-important-members-of-mercury-methylating-microbial-communities-of-sediments-impacted-by-waste-water-releases
#5
Andrea G Bravo, Jakob Zopfi, Moritz Buck, Jingying Xu, Stefan Bertilsson, Jeffra K Schaefer, John Poté, Claudia Cosio
Microbial mercury (Hg) methylation in sediments can result in bioaccumulation of the neurotoxin methylmercury (MMHg) in aquatic food webs. Recently, the discovery of the gene hgcA, required for Hg methylation, revealed that the diversity of Hg methylators is much broader than previously thought. However, little is known about the identity of Hg-methylating microbial organisms and the environmental factors controlling their activity and distribution in lakes. Here, we combined high-throughput sequencing of 16S rRNA and hgcA genes with the chemical characterization of sediments impacted by a waste water treatment plant that releases significant amounts of organic matter and iron...
January 10, 2018: ISME Journal
https://www.readbyqxmd.com/read/29320234/influence-of-soap-characteristics-and-food-service-facility-type-on-the-degree-of-bacterial-contamination-of-open-refillable-bulk-soaps
#6
Donald W Schaffner, Dane Jensen, Charles P Gerba, David Shumaker, James W Arbogast
Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29315383/multiple-intraintestinal-signals-coordinate-the-regulation-of-vibrio-cholerae-virulence-determinants
#7
Kenneth M Peterson, Patrick S Gellings
Vibrio cholerae is a gram-negative motile bacterium capable of causing fatal pandemic disease in humans via oral ingestion of contaminated water or food. Within the human intestine the motile vibrios must evade the innate host defense mechanisms, penetrate the mucus layer covering the small intestine, adhere to and multiply on the surface of the microvilli and cause disease via the action of cholera toxin. The explosive diarrhea associated with V. cholerae intestinal colonization leads to dissemination of the vibrios back into the environment to complete this phase of the life cycle...
January 5, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29314166/ecotoxicology-of-manufactured-graphene-oxide-nanomaterials-and-derivation-of-preliminary-guideline-values-for-freshwater-environments
#8
Marijana Markovic, Anupama Kumar, Ivan Andjelkovic, Supriya Lath, Jason K Kirby, Dusan Losic, Graeme E Batley, Michael J McLaughlin
The unique physical and chemical properties of graphene-based nanomaterials (GNMs) have inspired a diverse range of scientific and industrial applications. The market value of GNMs is predicted to reach $US 1.3 billion by 2023. Common to many nanomaterials (NMs), an important and unresolved question is the environmental consequences of the increases in GNM use. The current deficiencies in studies reporting ecotoxicology data for GNMs include, differences in analytical methodologies for quantification, no standardized test guidelines, differences in morphology of GNMs, the lack of Chemical Abstract Service (CAS) numbers and the quality of the reported data...
January 4, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29306652/influence-of-the-surrounding-environment-in-re-naturalized-%C3%AE-barrel-membrane-proteins
#9
Maximilien Lopes-Rodrigues, Jordi Triguero, Juan Torras, Eric A Perpète, Catherine Michaux, David Zanuy, Carlos Alemán
Outer-membrane porins are currently being used to prepare bioinspired nanomembranes for selective ion transport by immobilizing them into polymeric matrices. However, the fabrication of these protein-integrated devices has been found to be strongly influenced by the instability of the β-barrel porin structure, which depends on surrounding environment. In this work, molecular dynamics simulations have been used to investigate the structural stability of a representative porin, OmpF, in three different environments: (i) aqueous solution at pH=7; (ii) a solution of neutral detergent in a concentration similar to the critical micelle concentration; and (iii) the protein embedded into a neutral detergent bilayer...
January 2, 2018: Biophysical Chemistry
https://www.readbyqxmd.com/read/29306393/seasonal-dynamics-of-microcystin-degrading-bacteria-and-toxic-cyanobacterial-blooms-interaction-and-influence-of-abiotic-factors
#10
María Ángeles Lezcano, Antonio Quesada, Rehab El-Shehawy
Massive proliferations of cyanobacteria coexist and have different interactions with other microorganisms, including microcystin (MC)-degrading bacteria. Despite their relevance in the environment for the removal of MCs, this bacterial community has been scarcely studied. The influence of physicochemical factors and the seasonal dynamics of toxic cyanobacteria on the relative abundance and seasonal dynamics of the MC-degrading bacterial community with mlr genes (mlr+) were investigated during a two-year study at a water reservoir in central Spain...
January 2018: Harmful Algae
https://www.readbyqxmd.com/read/29305724/tools-for-monitoring-aquatic-environments-to-identify-anthropic-effects
#11
Monyque Palagano da Rocha, Priscila Leocadia Rosa Dourado, Claudia Andrea Lima Cardoso, Liliam Silva Cândido, Joelson Gonçalves Pereira, Kelly Mari Pires de Oliveira, Alexeia Barufatti Grisolia
Anthropic activities are directly related to the contamination of aquatic ecosystems owing to the release of numerous chemicals from agricultural and urban waste. These contaminants cause environmental degradation and a decrease in the availability of water quality. The objective of this search was to evaluate the efficiency of physicochemical, chemical, and microbiological tests; extraction of chlorophyll a; and genetic parameters to identify anthropic activities and weather condition effects on the stream water quality and the consequences of its use by the population...
January 5, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29303410/relationship-of-nutrient-dynamics-and-bacterial-community-structure-at-the-water-sediment-interface-using-a-benthic-chamber-experiment
#12
Bo-Min Ki, In Ae Huh, Jung-Hyun Choi, Kyung-Suk Cho
The relationships between nutrient dynamics and the bacterial community at the water-sediment interface were investigated using the results of nutrient release fluxes, bacterial communities examined by 16S rRNA pyrosequencing and canonical correlation analysis (CCA) accompanied by lab-scale benthic chamber experiment. The nutrient release fluxes from the sediments into the water were as follows: -3.832 to 12.157 mg m-2 d-1 for total phosphorus, 0.049 to 9.993 mg m-2 d-1 for PO4-P, -2.011 to 41.699 mg m-2 d-1 for total nitrogen, -7...
January 5, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29300761/investigation-of-prevalence-of-free-shiga-toxin-producing-escherichia-coli-stec-specific-bacteriophages-and-its-correlation-with-stec-bacterial-hosts-in-a-produce-growing-area-in-salinas-california
#13
Yen-Te Liao, Irwin A Quintela, Kimberly Nguyen, Alexandra Salvador, Michael B Cooley, Vivian C H Wu
Shiga toxin-producing E. coli (STEC) causes approximately 265,000 illnesses and 3,600 hospitalizations annually and is highly associated with animal contamination due to the natural reservoir of ruminant gastrointestinal tracts. Free STEC-specific bacteriophages against STEC strains are also commonly isolated from fecal-contaminated environment. Previous studies have evaluated the correlation between the prevalence of STEC-specific bacteriophages and STEC strains to improve animal-associated environment. However, the similar information regarding free STEC-specific bacteriophages prevalence in produce growing area is lacking...
2018: PloS One
https://www.readbyqxmd.com/read/29290482/first-report-on-extended-spectrum-beta-lactamase-esbl-producing-escherichia-coli-from-european-free-tailed-bats-tadarida-teniotis-in-portugal-a-one-health-approach-of-a-hidden-contamination-problem
#14
Andreia Garcês, Susana Correia, Francisco Amorim, José Eduardo Pereira, Gilberto Igrejas, Patrícia Poeta
The main aim of this study was to characterize the diversity of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli isolates from European free tailed-bats (Tadarida teniotis) in Portugal. ESBL-producing E. coli isolates were recovered from 14 of 146 faecal samples (9.6%) and a total of 19 isolates were completely characterized. The more prevalent beta-lactamase genes detected were blaCTX-M-1 (57.9%) and blaCTX-M-3 (36.8%), followed by blaSHV (31.6%), blaTEM (21.1%), blaOXA (10.5%) and blaCTX-M-9 (10...
December 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29284053/esbl-producing-escherichia-coli-in-swedish-gulls-a-case-of-environmental-pollution-from-humans
#15
Clara Atterby, Stefan Börjesson, Sofia Ny, Josef D Järhult, Sara Byfors, Jonas Bonnedahl
ESBL-producing bacteria are present in wildlife and the environment might serve as a resistance reservoir. Wild gulls have been described as frequent carriers of ESBL-producing E. coli strains with genotypic characteristics similar to strains found in humans. Therefore, potential dissemination of antibiotic resistance genes and bacteria between the human population and wildlife need to be further investigated. Occurrence and characterization of ESBL-producing E. coli in Swedish wild gulls were assessed and compared to isolates from humans, livestock and surface water collected in the same country and similar time-period...
2017: PloS One
https://www.readbyqxmd.com/read/29280153/methods-for-identification-and-differentiation-of-different-shewanella-spp-isolates-for-diagnostic-use
#16
V Jung-Schroers, A Jung, M Ryll, J Bauer, F Teitge, D Steinhagen
Shewanella spp. are Gram-negative, rod-shaped, motile bacteria that are widely distributed in marine and freshwater environments. The bacteria are present in the physiological microflora of fish from temperate waters and are known as fish spoilage species. From clinically healthy fish and from fish with skin ulcerations, Shewanella spp. is regularly isolated, indicating a possible role as fish pathogen. In this study, 74 isolates of Shewanella spp. were analysed. For species identification, biochemical techniques, 16S rRNA sequencing, MALDI-TOF MS and the Sherlock Microbial Identification System (MIS) based on the composition of fatty acid ethyl esters were compared...
December 27, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29276774/effect-of-environmental-factors-and-an-emerging-parasitic-disease-on-gut-microbiome-of-wild-salmonid-fish
#17
Anti Vasemägi, Marko Visse, Veljo Kisand
The gastrointestinal tract (GIT) of fish supports a dynamic microbial ecosystem that is intimately linked to host nutrient acquisition, epithelial development, immune system priming, and disease prevention, and we are far from understanding the complex interactions among parasites, symbiotic gut bacteria, and host fitness. Here, we analyzed the effects of environmental factors and parasitic burdens on the microbial composition and diversity within the GIT of the brown trout (Salmo trutta). We focused on the emerging dangerous salmonid myxozoan parasite Tetracapsuloides bryosalmonae, which causes proliferative kidney disease in salmonid fish, to demonstrate the potential role of GIT micobiomes in the modulation of host-parasite relationships...
November 2017: MSphere
https://www.readbyqxmd.com/read/29273919/microbial-diversity-of-saline-environments-searching-for-cytotoxic-activities
#18
Carolina Díaz-Cárdenas, Angela Cantillo, Laura Yinneth Rojas, Tito Sandoval, Susana Fiorentino, Jorge Robles, Freddy A Ramos, María Mercedes Zambrano, Sandra Baena
In order to select halophilic microorganisms as a source of compounds with cytotoxic activities, a total of 135 bacterial strains were isolated from water and sediment samples collected from the Zipaquirá salt mine in the Colombian Andes. We determined the cytotoxic effects of 100 crude extracts from 54 selected organisms on the adherent murine mammary cell carcinoma 4T1 and human mammary adenocarcinoma MCF-7 cell lines. These extracts were obtained from strains of Isoptericola, Ornithinimicrobium, Janibacter, Nesterenkonia, Alkalibacterium, Bacillus, Halomonas, Chromohalobacter, Shewanella, Salipiger, Martellela, Oceanibaculum, Caenispirillum and Labrenzia...
December 22, 2017: AMB Express
https://www.readbyqxmd.com/read/29261428/first-characterization-of-pah-degrading-bacteria-from-r%C3%A3-o-de-la-plata-and-high-resolution-melting-an-encouraging-step-towards-bioremediation
#19
Silvina A Izzo, Silvina Quintana, Mariela Espinosa, Paola A Babay, Silvia R Peressutti
The Río de la Plata, one of the most important estuarine environments in South America that sustains valuable fisheries, is affected by PAH contamination associated to oil industry and port activities. A total of 95 bacteria with potential to degrade phenanthrene were obtained from water samples using traditional culture methods. PCR-RFLP analysis of 16S rDNA partial fragments was used as a screening tool for reducing the number of isolates during diversity studies, obtaining 42 strains with different fingerprint pattern...
December 20, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29260261/microalgae-bacteria-biofilms-a-sustainable-synergistic-approach-in-remediation-of-acid-mine-drainage
#20
REVIEW
Sudharsanam Abinandan, Suresh R Subashchandrabose, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Microalgae and bacteria offer a huge potential in delving interest to study and explore various mechanisms under extreme environments. Acid mine drainage (AMD) is one such environment which is extremely acidic containing copious amounts of heavy metals and poses a major threat to the ecosystem. Despite its extreme conditions, AMD is the habitat for several microbes and their activities. The use of various chemicals in prevention of AMD formation and conventional treatment in a larger scale is not feasible under different geological conditions...
December 19, 2017: Applied Microbiology and Biotechnology
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