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water bacteria

Julian Bejarano, Rainer Detsch, Aldo R Boccaccini, Humberto Palza
Novel multifunctional scaffolds for bone regeneration can be developed by incorporation of bioactive glasses doped with therapeutic and antibacterial metal ions, such as copper (Cu) and zinc (Zn), into a biodegradable polymer. In this context, porous composite materials of biodegradable poly(D, L-lactide) (PDLLA) mixed with sol-gel bioactive glasses (BG) of chemical composition 60SiO2 ; 25CaO; 11Na2 O; and 4P2 O5 (mol.%) doped with either 1 mol% of CuO or ZnO, and with both metals, were prepared. The cytocompatibility of the scaffolds on bone marrow stromal cells (ST-2) depended on both, the amount of glass filler and the concentration of metal ion, as evaluated by lactate dehydrogenase (LDH) activity, cell viability (water-soluble tetrazolium salt (WST-8)), and by cell morphology (scanning electron microscopy (SEM)) tests...
October 26, 2016: Journal of Biomedical Materials Research. Part A
Moses Abiala, John Olayiwola, Oluwatoyin Babatunde, Olapeju Aiyelaagbe, Sunday Akinyemi
BACKGROUND: Plant extracts were evaluated on poultry bacteria known to be threatening public health. This is to develop better bio-therapeutic agents from plant origin. METHODS: Bacteria were isolated from water, feed, crop, gizzard and faeces of layer chicken. Isolates of interest (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa and Klebsiella oxytoca) were subjected to antibiotic susceptibility test. Resistant strains were further evaluated against different plant extracts in comparison to Meropenem (control) using agar diffusion method...
October 26, 2016: BMC Complementary and Alternative Medicine
Bailiang Wang, Zi Ye, Qingwen Xu, Huihua Liu, Quankui Lin, Hao Chen, Kaihui Nan
Once a biomedical implant is implanted into a human body, proteins and bacteria can easily colonize the implant, and subsequently, a biofilm can grow on the surface. A biofilm can protect the inhabiting bacteria against macrophages and neutrophil cell attack from the host immune system. The most important issue for artificial antibacterial surfaces is the accumulation of the bacteria corpse after they are killed by contact, which promotes further adhesion of bacteria and biofilm formation. Therefore, we constructed a novel multifunctional bactericidal and fouling release antibacterial surface through the combination of temperature-responsive N-vinylcaprolactam (VCL), hydrophilic 2-methacryloyloxyethyl phosphorylcholine (MPC) and a bactericidal quaternary ammonium salt (2-(dimethylamino)-ethyl methacrylate (DMAEMA(+)))...
October 26, 2016: Biomaterials Science
Songhe Zhang, Si Pang, Peifang Wang, Chao Wang, Chuan Guo, Felix Gyawu Addo, Yi Li
Submerged macrophytes play important roles in constructed wetlands and natural water bodies, as these organisms remove nutrients and provide large surfaces for biofilms, which are beneficial for nitrogen removal, particularly from submerged macrophyte-dominated water columns. However, information on the responses of biofilms to submerged macrophytes and nitrogen molecules is limited. In the present study, bacterial community structure and denitrifiers were investigated in biofilms on the leaves of four submerged macrophytes and artificial plants exposed to two nitrate concentrations...
October 26, 2016: Scientific Reports
Victoria M Pearson, S Brian Caudle, Darin R Rokyta
Understanding the structure and dynamics of microbial communities, especially those of economic concern, is of paramount importance to maintaining healthy and efficient microbial communities at agricultural sites and large industrial cultures, including bioprocessors. Wastewater treatment plants are large bioprocessors which receive water from multiple sources, becoming reservoirs for the collection of many viral families that infect a broad range of hosts. To examine this complex collection of viruses, full-length genomes of circular ssDNA viruses were isolated from a wastewater treatment facility using a combination of sucrose-gradient size selection and rolling-circle amplification and sequenced on an Illumina MiSeq...
2016: PeerJ
Murwantoko Murwantoko, Arif Bimantara, Roosmanto Roosmanto, Masashi Kawaichi
A pathogen of giant freshwater prawn, Macrobrachium rosenbergii, was recently recorded in a hatchery in Yogyakarta. The clinical symptom in post-larvae (PL) was a whitish appearance of the muscles in the tail. Histological examination revealed myonecrosis with massive infiltration of myonuclei and hemocytes. RT-PCR products of 850 bp were obtained when using RNA from diseased PL as a template. The clinical signs and RT-PCR amplicon were reproduced in M. rosenbergii inoculated with bacteria-free inocula. Electron microscopy demonstrated that the M...
2016: SpringerPlus
Sangwoo Kim, Wangjun Lee, Insu Song, Yuhyun Kwon, Seokhun Yun, Soohyun Park, Sukhyeong Cho, Byung-Keun Oh, Han Bin Oh, Jinwon Lee
Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu(2+) concentration and temperature were investigated and optimized...
October 21, 2016: Journal of Biotechnology
Elizabeth A Gonzalez, Poulomi Nandy, Anne D Lucas, Victoria M Hitchins
Cleaning reusable medical devices removes organic and inorganic soil, which allows for effective disinfection and sterilization. However, it is not always clear what variables to consider when validating cleaning. This study compared the ability of 3 different cleaning agents (ie, water, alcohol, and bleach) to remove bacteria (ie, vegetative and spores) and artificial blood test soil from 2 common device materials: polypropylene and ultra-high-molecular-weight polyethylene. There was a complex interaction between bacteria, soil, and surface roughness...
October 21, 2016: American Journal of Infection Control
S K Jesudoss, J Judith Vijaya, L John Kennedy, P Iyyappa Rajan, Hamad A Al-Lohedan, R Jothi Ramalingam, K Kaviyarasu, M Bououdina
The present work describes the successful synthesize of spinel magnetic ferrite Mn1-xNixFe2O4 (x=0.0, 0.1, 0.2, 0.3, 0.4 & 0.5) nanoparticles via a simple microwave combustion method which was then evaluated for its photocatalytic activity in the degradation of indigo carmine (IC) synthetic dye, a major water pollutant. Our results reveal that the synthesized of Ni(2+) doped MnFe2O4 nanoparticles possess well-crystalline pure cubic spinel phase, exhibit excellent optical and magnetic properties. Further, the photocatalytic performance of the synthesized nanoparticles at different concentration ratios of Ni(2+) ions was monitored by photocatalytic degradation of indigo carmine synthetic dye under UV (λ=365nm) light irradiation...
October 8, 2016: Journal of Photochemistry and Photobiology. B, Biology
Thomas B Atherholt, Nicholas A Procopio, Sandra M Goodrow
It is important that indicators of fecal pollution are reliable. Coliform bacteria are a commonly used indicator of fecal pollution. As other investigators have reported elsewhere, we observed a seasonal pattern of coliform bacteria detections in domestic wells in New Jersey. Examination of a statewide database of 10 years of water quality data from 93,447 samples, from 78,207 wells, generated during real estate transactions, revealed that coliform bacteria were detected in a higher proportion of wells during warm weather months...
October 24, 2016: Ground Water
Sten König, Olivier Gros, Stefan E Heiden, Tjorven Hinzke, Andrea Thürmer, Anja Poehlein, Susann Meyer, Magalie Vatin, Didier Mbéguié-A-Mbéguié, Jennifer Tocny, Ruby Ponnudurai, Rolf Daniel, Dörte Becher, Thomas Schweder, Stephanie Markert
The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO2 and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont-host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy)...
October 24, 2016: Nature Microbiology
Nayara Halimy Maran, Bruno do Amaral Crispim, Stephanie Ramirez Iahnn, Renata Pires de Araújo, Alexeia Barufatti Grisolia, Kelly Mari Pires de Oliveira
Use of groundwater from private wells in households has increased considerably, owing to a better cost/benefit ratio than that of water provided by local utilities for a fee. However, this water is usually untreated, which makes it a vehicle for diseases. Thus, monitoring this water is necessary to ensure its integrity and quality. We aimed to evaluate the physical, chemical, and microbiological parameters of untreated groundwater drawn from different types of wells, and the antimicrobial susceptibility profile of the bacteria isolated from this water...
October 21, 2016: International Journal of Environmental Research and Public Health
Nadine Czekalski, Stefanie Imminger, Elisabeth Salhi, Marjan Veljkovic, Karolin Kleffel, David Drissner, Frederik Hammes, Helmut Bürgmann, Urs von Gunten
Ozone, a strong oxidant and disinfectant, seems ideal to cope with future challenges of water treatment, such as micropollutants, multiresistant bacteria (MRB) and even intracellular antibiotic resistance genes (ARG), but information on the latter is scarce. In ozonation experiments we simultaneously determined kinetics and dose-dependent inactivation of Escherichia coli and its plasmid-encoded sulfonamide resistance gene sul1 in different water matrixes. Effects in E. coli were compared to an autochthonous wastewater community...
October 24, 2016: Environmental Science & Technology
Darija Vukić Lušić, Slaven Jozić, Arijana Cenov, Marin Glad, Marko Bulić, Dražen Lušić
Bathing Water Directive (2006/7/EC) specifies two reference methods for Escherichia coli detection: ISO 9308-1 and 9308-3. The revised ISO 9308-1 is recommended only for waters with a low bacterial background flora. Considering the extended time needed for analysis and, generally, the lack of experience in using ISO 9308-3 in the Mediterranean, the suitability of ISO 9308-1 for the examination of E. coli in bathing water was evaluated. The present study was aimed at a comparison of data obtained by the reference method in seawater samples (110 beaches, N=477) with data received from six alternative methods...
October 19, 2016: Marine Pollution Bulletin
Baoqing Zhou, Bolu Chen, Xin Wu, Fan Li, Pei Yu, Zoraida P Aguilar, Hua Wei, Hengyi Xu
A rapid, reliable, and sensitive method for the detection of Cronobacter sakazakii, a common foodborne pathogen that may cause serious neonatal disease, has been developed. In this study, a rapid real-time quantitative PCR (qPCR) assay combined with sodium deoxycholate (SD) and propidium monoazide (PMA) was developed to detect C. sakazakii contamination in powdered infant formula (PIF). This method could eliminate the interference from dead or injured bacteria. Optimization studies indicated that SD and PMA at 0...
October 19, 2016: Journal of Dairy Science
Anastasia V Nuzhdina, Alexey S Morozov, Maria N Kopitsyna, Elena N Strukova, Daria S Shlykova, Ivan V Bessonov, Elena S Lobakova
Novel quaternized polyethyleneimine and cross-linked polyethyleneimine derivatives have been synthesized using both traditional and microwave-assisted techniques to create antimicrobial coatings, with octyl, dodecyl, or hexadecyl bromides as alkylating agent and various bifunctional electrophiles as cross-linkers. Quaternization has been performed using methyl iodide or dimethyl sulfate; it has been shown that methyl iodide has no advantages over dimethyl sulfate. Antimicrobial activity of the polymers against Gram-positive (S...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Mustafa Nakipoğlu, Fadime Yilmaz, Bulent Icgen
Untreated wastewaters and treated effluents even after final disinfection contain antibiotic resistant bacteria and resistance genes before they are released into surface waters. A correlation between resistant bacteria and antibiotics in surface waters has been found, as have antibiotic resistance genes. Of particular interest are vancomycin-resistant enterococci harboring vanA gene that confers high level of resistance to glycopeptide antibiotics including teicoplanin. Therefore, in this study, river water samples were analysed to investigate vancomycin- and teicoplanin-resistant bacterial isolates harboring vanA gene...
October 21, 2016: Bulletin of Environmental Contamination and Toxicology
J F Leal, I S Henriques, A Correia, E B H Santos, V I Esteves
Oxytetracycline (OTC) is one of the most used antibiotics in aquaculture. The main concern related to its use is the bacterial resistance, when ineffective treatments are applied for its removal or inactivation. OTC photo-degradation has been suggested as an efficient complementary process to conventional methods used in intensive fish production (e.g.: ozonation). Despite this, and knowing that the complete mineralization of OTC is difficult, few studies have examined the antibacterial activity of OTC photoproducts...
October 18, 2016: Environmental Pollution
Hongli Li, Victoria M Hitchins, Samanthi Wickramasekara
Bacterial endotoxins are lipopolysaccharides bound to the bacterial cell wall and released when bacteria rupture or disintegrate. Possible contamination of endotoxin in ophthalmic devices can cause a painful eye inflammation or result in toxic anterior segment syndrome after cataract surgery. Measurement of bacterial endotoxin in medical device materials is difficult since endotoxin binds with polymer matrix and some of the materials are very viscous and non-water soluble, where traditional enzyme-based Limulus amebocyte lysate (LAL) assay cannot be applied...
November 2, 2016: Analytica Chimica Acta
Jasmine S Berg, Dolma Michellod, Petra Pjevac, Clara Martinez-Perez, Caroline R T Buckner, Philipp F Hach, Carsten J Schubert, Jana Milucka, Marcel M M Kuypers
Iron redox reactions play an important role in carbon remineralization, supporting large microbial communities in iron-rich terrestrial and aquatic sediments. Stratified water columns with comparably low iron concentrations are globally widespread, but microbial iron cycling in these systems has largely been ignored. We found evidence for unexpectedly high iron turnover rates in the low (1-2 µmol·l(-1) ) iron waters of Lake Cadagno. Light-dependent, biological iron oxidation rates (1.4-13.8 µmol·l(-1) ·d(-1) ) were even higher than in ferruginous lakes with well-studied microbial iron cycles...
October 21, 2016: Environmental Microbiology
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