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https://www.readbyqxmd.com/read/29779101/the-role-of-epibionts-of-bacteria-of-the-genus-pseudoalteromonas-and-cellular-proteasomes-in-the-adaptive-plasticity-of-marine-cold-water-sponges
#1
O I Kravchuk, A I Lavrov, A D Finoshin, N G Gornostaev, A A Georgiev, S B Abaturova, V S Mikhailov, Yu V Lyupina
It was found that cells of different color morphs of the cold-water marine sponges Halichondria panicea (Pallas, 1766) of the class Demospongiae differ in the content of epibionts of bacteria of the genus Pseudoalteromonas. The sponge cells with elevated levels of epibionts of bacteria of the genus Pseudoalteromonas showed an increased expression of Hsp70 proteins but had a reduced level of the proteasomal catalytic beta 5 subunit, which was accompanied by a change in their activity. Probably, epibionts of bacteria of the genus Pseudoalteromonas may affect the ubiquitin-proteasome system in the cells of cold-water marine sponges and, thereby, ensure their adaptive plasticity...
March 2018: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29778431/microbiologic-assessment-of-flexible-gastrointestinal-endoscope-reprocessing-using-a-pump-assisted-sampling-technique-an-investigation-involving-all-endoscopy-units-in-tianjin-china
#2
Xue-Yue Ji, Pei-Yong Ning, Wei Zhang, Dong-Jing Yang, He Liu, Jun Liu, Jia Song, Chun-Nan Fei
BACKGROUND: Microbiologic surveillance of flexible gastrointestinal endoscopes is recommended in several guidelines as the primary means of identifying reprocessing failures. This study aimed to evaluate the contamination level and prevalence of bacteria of post-reprocessing endoscopes and to access whether using a pump-assisted sampling method (PASM) improves the sensitivity of culture. METHODS: All 59 endoscopy units in Tianjin, China, were investigated. The PASM and the conventional flushing sampling method (CFSM) were used to compare the results of the microbial culture...
May 16, 2018: American Journal of Infection Control
https://www.readbyqxmd.com/read/29778067/succession-of-bacterial-and-fungal-communities-within-biofilms-of-a-chlorinated-drinking-water-distribution-system
#3
I Douterelo, K E Fish, J B Boxall
Understanding the temporal dynamics of multi-species biofilms in Drinking Water Distribution Systems (DWDS) is essential to ensure safe, high quality water reaches consumers after it passes through these high surface area reactors. This research studied the succession characteristics of fungal and bacterial communities under controlled environmental conditions fully representative of operational DWDS. Microbial communities were observed to increase in complexity after one month of biofilm development but they did not reach stability after three months...
May 3, 2018: Water Research
https://www.readbyqxmd.com/read/29778035/formation-of-contact-active-antimicrobial-surfaces-by-covalent-grafting-of-quaternary-ammonium-compounds
#4
REVIEW
Poverenov Elena, Klein Miri
Different synthetic strategies for the formation of contact active antimicrobial materials utilizing covalent linkage of quaternary ammonium compounds (QACs) were reviewed. There is a demand to find methods that will prevent bacterial fouling without the release of antimicrobial agents, because biocides cause environment pollution and promote the development of bacteria resistance mechanisms. The contact active antimicrobial surfaces may provide a useful tool for this purpose. The covalent surface grafting of QACs seems to be a feasible and promising approach for the formation of safe and effective antimicrobial materials that could be utilized for medical devices, food industry, water treatment systems and other applications...
May 16, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29777948/escherichia-coli-hyperepidemic-clone-st410-a-harboring-bla-ctx-m-15-isolated-from-fresh-vegetables-in-a-municipal-market-in-quito-ecuador
#5
David Ortega-Paredes, Pedro Barba, Santiago Mena-López, Nathaly Espinel, Jeannete Zurita
Dissemination of Extended spectrum β-lactamases (ESBL) Enterobacteriaceae is a major medical threat. Vegetables and fruits, which are usually consumed raw, are a very suitable pathway for the spread of these bacteria from farm-to-fork. However, limited information exists regarding resistant bacteria and epidemic clones that are disseminated in vegetables and tap water in South America. We processed a total of 90 samples in triplicate of nine typically consumed raw vegetables from a central municipal market, and tap water samples were processed from twenty-one locations in Quito, Ecuador...
April 30, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29777807/identification-of-polysaccharides-extracted-from-pea-pod-by-products-and-evaluation-of-their-biological-and-functional-properties
#6
Lilia Belghith-Fendri, Fatma Chaari, Khawla Ben Jeddou, Fatma Kallel, Fatma Bouaziz, Claire Boisset Helbert, Lobna Abdelkefi-Mesrati, Semia Ellouz-Chaabouni, Dhouha Ghribi-Aydi
A three-variable Box-Behnken design was employed to obtain the best possible combination of extraction time, ratio (raw material/water) and extraction temperature to allow maximum extraction yield of polysaccharides from pea pod (PPP). The preferred extraction conditions were: extraction time 195 min, extraction temperature 70 °C and ratio of raw material/water 1/40. Under these conditions, the experimental yield was 16.21 ± 1.12%, which is in close agreement with the value predicted by response surface methodology model yield (16...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29776926/occurrence-of-traditional-and-alternative-fecal-indicators-in-tropical-urban-environments-under-different-land-use-patterns
#7
Nazanin Saeidi, Xiaoqiong Gu, Tran Ngoc Han, Shin Giek Goh, Masaaki Kitajima, Ariel Kushmaro, Bradley William Schmitz, Karina Yew-Hoong Gin
This study evaluates the geospatial distribution of fecal indicator bacteria (FIB) (i.e., E. coli , Enterococcus spp.) and the alternative fecal indicator, pepper mild mottle virus (PMMoV), in tropical freshwater environments under different land use patterns. Results show that the occurrence and concentration of microbial fecal indicators was higher for urban-dominated areas than parkland-dominated, consistent with land use weightage. Significant positive correlations with traditional FIB indicate that PMMoV is a suitable indicator of fecal contamination in tropical catchments waters (0...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29774019/long-term-effects-of-residual-chlorine-on-pseudomonas-aeruginosa-in-simulated-drinking-water-fed-with-low-aoc-medium
#8
Guannan Mao, Yuhao Song, Mark Bartlam, Yingying Wang
Residual chlorine is often required to remain present in public drinking water supplies during distribution to ensure water quality. It is essential to understand how bacteria respond to long-term chlorine exposure, especially with the presence of assimilable organic carbon (AOC). This study aimed to investigate the effects of chlorination on Pseudomonas aeruginosa in low AOC medium by both conventional plating and culture-independent methods including flow cytometry (FCM) and quantitative PCR (qPCR). In a simulated chlorinated system using a bioreactor, membrane damage and DNA damage were measured by FCM fluorescence fingerprint...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29773926/comparative-analysis-of-airborne-bacteria-and-fungi-in-two-salt-mines-in-poland
#9
Elżbieta Gębarowska, Wojciech Pusz, Jolanta Kucińska, Włodzimierz Kita
The aim of this work was to determine the genera or species composition and the number of colony forming units of airborne bacteria and fungi, respectively, in two salt mines in Poland "Wieliczka" (Lesser Poland) and "Polkowice-Sieroszowice" (Lower Silesia). Both of them are working environments characterized by extreme conditions, and additionally "Wieliczka," officially placed on the UNESCO World Heritage Sites' list, plays a role of tourist attraction. There are also some curative chambers located in this mine...
2018: Aerobiologia
https://www.readbyqxmd.com/read/29771118/escherichia-coli-fiber-sensors-using-concentrated-dielectrophoretic-force-with-optical-defocusing-method
#10
Yi-Hsin Tai, Chia-Wei Lee, Dao-Ming Chang, Yu-Sheng Lai, Ding-Wei Huang, Pei-Kuen Wei
A sensitive tapered optical fiber tip combined with dielectrophoretic (DEP) trapping was used to rapid and label-free detection of bacteria in water. The angular spectrum of optical field at the fiber tip was changed with surrounding refractive index (RI). By measuring far-field intensity change at the defocus plane, the intensity sensitivity was up to 95,200 %/RIU (RI unit) and the detection limit was 2.1 × 10-6 RIU at 0.2% intensity stability. By applying an AC voltage to a Ti/Al coated fiber tip and an indium-tin-oxide glass, the DEP force effectively trapped the Escherichia coli (E...
May 17, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29770941/degradation-of-ampicillin-antibiotic-by-electrochemical-processes-evaluation-of-antimicrobial-activity-of-treated-water
#11
Jorge Vidal, Cesar Huiliñir, Rocío Santander, Javier Silva-Agredo, Ricardo A Torres-Palma, Ricardo Salazar
Ampicillin (AMP) is an antibiotic widely used in hospitals and veterinary clinics around the world for treating infections caused by bacteria. Therefore, it is common to find traces of this antibiotic in wastewater from these entities. In this work, we studied the mineralization of this antibiotic in solution as well as the elimination of its antimicrobial activity by comparing different electrochemical advanced oxidation processes (EAOPs), namely electro-oxidation with hydrogen peroxide (EO-H2 O2 ), electro-Fenton (EF), and photo electro-Fenton (PEF)...
May 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29770540/efficient-adsorption-characteristics-of-pristine-and-silver-doped-graphene-oxide-towards-contaminants-a-novel-approach-to-water-purification-mechanism
#12
Tanveer Hussain, Puspamitra Panigrahi, Ashok Kumar Dhinakaran, Yuvaraj Sekar, Rajeev Ahuja
In this work, we have investigated the potential of pristine and silver (Ag) functionalized graphene oxide monolayers GO (GO-Ag) as efficient membranes for water filtration and separation. Our first principles calculation based on density functional theory (DFT) reveled the hydrophilic nature of GO surfaces. The phonon frequency calculations within density functional perturbation theory (DFPT) confirmed the stability of GO sheets in the aqueous medium. Van der Waals corrected binding energies of GO sheet towards the heavy metals predicts that even pristine GO sheets would be completely impermeable to various heavy metals like arsenic (As) and lead (Pb)...
May 16, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29769716/mutant-phenotypes-for-thousands-of-bacterial-genes-of-unknown-function
#13
Morgan N Price, Kelly M Wetmore, R Jordan Waters, Mark Callaghan, Jayashree Ray, Hualan Liu, Jennifer V Kuehl, Ryan A Melnyk, Jacob S Lamson, Yumi Suh, Hans K Carlson, Zuelma Esquivel, Harini Sadeeshkumar, Romy Chakraborty, Grant M Zane, Benjamin E Rubin, Judy D Wall, Axel Visel, James Bristow, Matthew J Blow, Adam P Arkin, Adam M Deutschbauer
One-third of all protein-coding genes from bacterial genomes cannot be annotated with a function. Here, to investigate the functions of these genes, we present genome-wide mutant fitness data from 32 diverse bacteria across dozens of growth conditions. We identified mutant phenotypes for 11,779 protein-coding genes that had not been annotated with a specific function. Many genes could be associated with a specific condition because the gene affected fitness only in that condition, or with another gene in the same bacterium because they had similar mutant phenotypes...
May 16, 2018: Nature
https://www.readbyqxmd.com/read/29768968/susceptibility-of-gut-indigenous-lactic-acid-bacteria-in-balb-c-mice-to-oral-administered-lactobacillus-plantarum
#14
Takashi Kuda, Yasushi Yokota, Yutaka Haraguchi, Hajime Takahashi, Bon Kimura
Cells of Lactobacillus plantarum strains AN1 and Tennozu-SU2 exert anti-inflammatory responses in ICR mouse models of inflammatory bowel disease and protective effects against S. Typhimurium infection in BALB/c mice, respectively. To clarify the existence of L. plantarum-susceptible gut indigenous bacteria, AN1 and Tennozu-SU2 cells were administered to BALB/c mice via drinking water. Gene amplicon sequencing of 16S rRNA of caecal content revealed that the AN1 and Tennozu-SU2 cells affected the abundance of caecal indigenous lactobacilli, but the effect on the dominant Clostridiales and Bacteroidales was not clear...
May 16, 2018: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/29768658/detrimental-effects-of-rhizobial-inoculum-early-in-the-life-of-partridge-pea-chamaecrista-fasciculata
#15
Rachel E Pain, Ruth G Shaw, Seema N Sheth
PREMISE OF THE STUDY: Mutualistic relationships with microbes may aid plants in overcoming environmental stressors and increase the range of abiotic environments where plants can persist. Rhizobia, nitrogen-fixing bacteria associated with legumes, often confer fitness benefits to their host plants by increasing access to nitrogen in nitrogen-limited soils, but effects of rhizobia on host fitness under other stresses, such as drought, remain unclear. METHODS: In this greenhouse study, we varied the application of rhizobia (Bradyrhizobium sp...
May 16, 2018: American Journal of Botany
https://www.readbyqxmd.com/read/29766332/ecofriendly-demulsification-of-water-in-oil-emulsions-by-an-efficient-biodemulsifier-producing-bacterium-isolated-from-oil-contaminated-environment
#16
Hoda Sabati, Hossein Motamedi
OBJECTIVE: Water in oil emulsions increase oil processing costs and cause damage to refinery equipment which necessitates demulsification. Since chemical demulsifiers cause environmental pollution, biodemulsifiers have been paid more attention. This study aims to identify biodemulsifier-producing bacteria from petroleum contaminated environments. RESULTS: As a result, several biodemulsifier producing strains were found that Stenotrophomonas sp. strain HS7 (accession number: MF445088) which produced a cell associated biodemulsifier showed the highest demulsifying ratio, 98...
May 15, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29765365/human-activity-determines-the-presence-of-integron-associated-and-antibiotic-resistance-genes-in-southwestern-british-columbia
#17
Miguel I Uyaguari-Díaz, Matthew A Croxen, Zhiyao Luo, Kirby I Cronin, Michael Chan, Waren N Baticados, Matthew J Nesbitt, Shaorong Li, Kristina M Miller, Damion Dooley, William Hsiao, Judith L Isaac-Renton, Patrick Tang, Natalie Prystajecky
The dissemination of antibiotic resistant bacteria from anthropogenic sources into the environment poses an emerging public health threat. Antibiotic resistance genes (ARGs) and gene-capturing systems such as integron-associated integrase genes ( intI ) play a key role in alterations of microbial communities and the spread of antibiotic resistant bacteria into the environment. In order to assess the effect of anthropogenic activities on watersheds in southwestern British Columbia, the presence of putative antibiotic resistance and integrase genes was analyzed in the microbiome of agricultural, urban influenced, and protected watersheds...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29765364/rearing-water-treatment-induces-microbial-selection-influencing-the-microbiota-and-pathogen-associated-transcripts-of-cod-gadus-morhua-larvae
#18
Ragnhild I Vestrum, Kari J K Attramadal, Per Winge, Keshuai Li, Yngvar Olsen, Atle M Bones, Olav Vadstein, Ingrid Bakke
We have previously shown that K-selection and microbial stability in the rearing water increases survival and growth of Atlantic cod ( Gadus morhua ) larvae, and that recirculating aquaculture systems (RAS) are compatible with this. Here, we have assessed how water treatment influenced the larval microbiota and host responses at the gene expression level. Cod larvae were reared with two different rearing water systems: a RAS and a flow-through system (FTS). The water microbiota was examined using a 16S rDNA PCR/DGGE strategy...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29765086/fabrication-and-characterization-of-a-scalable-surface-textured-with-pico-liter-oil-drops-for-mechanistic-studies-of-bacteria-oil-interactions
#19
Maryam Jalali, Andrew R White, James Marti, Jian Sheng
Texturing a large surface with oily micro-drops with controlled size, shape and volume provides an unprecedented capability in investigating complex interactions of bacteria, cells and interfaces. It has particular implications in understanding key microbial processes involved in remediation of environmental disasters, such as Deepwater Horizon oil spill. This work presents a development of scalable micro-transfer molding to functionalize a substrate with oily drop array to generate a microcosm mimicking bacteria encountering a rising droplet cloud...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29763800/potentials-of-using-mixed-culture-bacteria-incorporated-with-sodium-bicarbonate-for-hydrogen-production-from-water-hyacinth
#20
Alaa Wazeri, Mohamed Elsamadony, Sophie Le Roux, Pascal Peu, Ahmed Tawfik
The aim of this study is to assess the potentials of using mixed culture bacteria incorporated with different concentrations of NaHCO3 for hydrogen production from water hyacinth (WH). The lowest hydrogen yield (HY) of 30.4 ± 1.9 mL/gTVS , H2 content (HC) of 19.5 ± 1.5% and hydrogenase enzyme (HE) activity of 0.06 ± 0.01 mgM.Breduced /min were registered for the cultures without supplementation of NaHCO3 . The HY, HC, and HE activity were maximized at levels of 69.2 ± 4.3 mL/gTVS, 58...
May 8, 2018: Bioresource Technology
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