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https://www.readbyqxmd.com/read/28742878/assessment-of-the-effect-of-enteromorpha-prolifera-on-bacterial-community-structures-in-aquaculture-environment
#1
Guorong Lin, Fulin Sun, Chunzhong Wang, Li Zhang, Xinzhong Zhang
In recent years, Enteromorpha prolifera blooms had serious impacts on costal environments and fisheries in China. Nevertheless, the effects of E. prolifera on microbial ecology remain unknown. In this study, for the first time, an Illumina sequencing analysis was used to investigate bacterial communities in source water, aquaculture ponds with E. prolifera, and an aquaculture pond in which E. prolifera -free. Principal coordinate and phylogenic analyses revealed obvious differences among the bacterial communities in the pond water with and without E...
2017: PloS One
https://www.readbyqxmd.com/read/28742284/aquatic-animals-promote-antibiotic-resistance-gene-dissemination-in-water-via-conjugation-role-of-different-regions-within-the-zebra-fish-intestinal-tract-and-impact-on-fish-intestinal-microbiota
#2
Jialun Fu, Dong Yang, Min Jin, Weili Liu, Xin Zhao, Chenyu Li, Tianyu Zhao, Jingfeng Wang, Zhixian Gao, Zhiqiang Shen, Zhigang Qiu, Jun-Wen Li
The aqueous environment is one of many reservoirs of antibiotic resistance genes (ARGs). Fish, as important aquatic animals which possess ideal intestinal niches for bacteria to grow and multiply, may ingest antibiotic resistance bacteria from aqueous environment. The fish gut would be a suitable environment for conjugal gene transfer including those encoding antibiotic resistance. However, little is known in relation to the impact of ingested ARGs or ARB on gut microbiota. Here, we applied the cultivation method, qPCR, nuclear molecular genetic marker and 16S rDNA amplicon sequencing technologies to develop a plasmid-mediated ARG transfer model of zebrafish...
July 25, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28739413/production-of-recombinant-flagellin-to-develop-elisa-based-detection-of-salmonella-enteritidis
#3
Seyed Ali Mirhosseini, Abbas Ali Imani Fooladi, Jafar Amani, Hamid Sedighian
Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen...
July 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28739115/a-recombinant-protein-of-salmonella-typhi-induces-humoral-and-cell-mediated-immune-responses-including-memory-responses
#4
Sayan Das, Rimi Chowdhury, Shubhamoy Ghosh, Santasabuj Das
Gram negative enteric bacteria, Salmonella enterica serovar Typhi (S. Typhi), the etiological agent of typhoid fever is a major public health problem in developing countries. While a permanent solution to the problem would require improved sanitation, food and water hygiene, controlling the infection by vaccination is urgently required due to the emergence of multidrug resistant strains in multiple countries. The currently licensed vaccines are moderately efficacious with limited applicability, and no recommended vaccines exist for younger children...
July 21, 2017: Vaccine
https://www.readbyqxmd.com/read/28738172/impact-of-polymeric-membrane-breakage-on-drinking-water-quality-and-an-online-detection-method-of-the-breakage
#5
Qilong Wu, Zhenghua Zhang, Guodong Cao, Xihui Zhang
Polymeric membrane has been widely used for the treatment of drinking water in China, and the total treating capacity has reached up to 3.8 million m(3)/d. However, the membrane breakage found in the membrane modules in many water treatment plants resulted in an increase in turbidity and bacterial amount in the membrane permeate. In this study, a membrane module running for 3 years in a full-scale application was examined in terms of the breaking positions and the numbers of the broken fibers. It was found that most of the breaking positions were mainly on the outlet side of the module and that the distance from these points to the outlet was about 1/10-2/10 length of the membrane module...
July 24, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28738078/evaluation-of-maldi-tof-mass-spectrometry-and-maldi-biotyper-in-comparison-to-16s-rdna-sequencing-for-the-identification-of-bacteria-isolated-from-arctic-sea-water
#6
Anna Maria Timperio, Susanna Gorrasi, Lello Zolla, Massimiliano Fenice
MALDI-TOF Mass Spectrometry in association with the MALDI BioTyper 3.1 software has been evaluated for the identification and classification of 45 Arctic bacteria isolated from Kandalaksha Bay (White Sea, Russia). The high reliability of this method has been already demonstrated, in clinical microbiology, by a number of studies showing high attribution concordance with other credited analyses. Recently, it has been employed also in other branches of microbiology with controversial performance. The phyloproteomic results reported in this study were validated with those obtained by the "gold standard" 16S rDNA analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28737669/molecular-dynamics-simulations-of-the-host-defense-peptide-temporin-l-and-its-q3k-derivative-an-atomic-level-view-from-aggregation-in-water-to-bilayer-perturbation
#7
Andrea Farrotti, Paolo Conflitti, Saurabh Srivastava, Jimut Kanti Ghosh, Antonio Palleschi, Lorenzo Stella, Gianfranco Bocchinfuso
Temporin L (TempL) is a 13 residue Host Defense Peptide (HDP) isolated from the skin of frogs. It has a strong affinity for lipopolysaccharides (LPS), which is related to its high activity against Gram-negative bacteria and also to its strong tendency to neutralize the pro-inflammatory response caused by LPS release from inactivated bacteria. A designed analog with the Q3K substitution shows an enhancement in both these activities. In the present paper, Molecular Dynamics (MD) simulations have been used to investigate the origin of these improved properties...
July 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28737160/health-and-environmental-risk-assessment-project-for-bottlenose-dolphins-tursiops-truncatus-from-the-southeastern-usa-ii-environmental-aspects
#8
REVIEW
John S Reif, Adam M Schaefer, Gregory D Bossart, Patricia A Fair
Bottlenose dolphins Tursiops truncatus are the most common apex predators found in coastal and estuarine ecosystems along the southeastern coast of the USA, where these animals are exposed to multiple chemical pollutants and microbial agents. In this review, we summarize the results of investigations of environmental exposures evaluated in 360 free-ranging dolphins between 2003 and 2015. Bottlenose dolphins inhabiting the Indian River Lagoon, Florida (IRL, n = 246), and coastal waters of Charleston, South Carolina (CHS, n = 114), were captured, given comprehensive health examinations, and released as part of a multidisciplinary and multi-institutional study of individual and population health...
July 24, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28736302/beyond-pegylation-alternative-surface-modification-of-nanoparticles-with-mucus-inert-biomaterials
#9
Vitaliy V Khutoryanskiy
Mucus is a highly hydrated viscoelastic gel present on various moist surfaces in our body including the eyes, nasal cavity, mouth, gastrointestinal, respiratory and reproductive tracts. It serves as a very efficient barrier that prevents harmful particles, viruses and bacteria from entering the human body. However, the protective function of the mucus also hampers the diffusion of drugs and nanomedicines, which dramatically reduces their efficiency. Functionalisation of nanoparticles with low molecular weight poly(ethylene glycol) (PEGylation) is one of the strategies to enhance their penetration through mucus...
July 20, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/28735316/biochar-rhizosphere-interactions-a-review
#10
Sławomir Głuszek, Lidia Sas Paszt, Beata Sumorok, Ryszard Kozera
Biochar is a solid material of biological origin obtained from the biomass carbonization, designed as a mean to reduce greenhouse gases emission and carbon sequestration in soils for a long time. Biochar has a wide spectrum of practical utilization and is applied as a promising soil improver or fertilizer in agriculture, or as a medium for soil or water remediation. Preparations of biochar increase plant growth and yielding when applied into soil and also improve plant growth conditions, mainly bio, physical and chemical properties of soil...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735314/gene-expression-during-btex-biodegradation-by-a-microbial-consortium-acclimatized-to-unleaded-gasoline-and-a-pseudomonas-putida-strain-hm346961-isolated-from-it
#11
Jesus A Morlett-Chavez, Jose A Ascacio-Martinez, William E Haskins, Karim Acuña-Askar, Hugo A Barrera-Saldaña
Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the P. putida strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. Furthermore, the toluene di-oxygenase (tod) gene was up-regulated in P. putida strain when exposed to toluene, ethylbenzene, xylene, and BTEX...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28734738/evaluation-of-the-biotechnological-potential-of-bacterioplankton-from-niter%C3%A3-i-coast-rj
#12
Marcelly Miranda Aybal Jayme, Rebeca Oliveira Castro, Carlos André Mandarino Silva, Mariana Muniz Silva, Flávia Lima do Carmo, Fábio Vieira de Araujo
Production of antibiotics and enzymes by microorganisms is well recognized for its economic benefits. Searching potentially exploitable microorganisms, strains of heterotrophic bacteria were isolated from marine and estuarine waters in Niterói-RJ, Brazil, and tested for the production of enzymes and antimicrobial substances. From the 76 isolated colonies, 09.21% showed antimicrobial potential, 63.16% showed enzymatic activity for at least one of the substrates tested and 91% showed multidrug resistance. Strains that presented the better results were identified by sequencing...
July 19, 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/28734568/antibacterial-properties-of-nano-silver-coated-peek-prepared-through-magnetron-sputtering
#13
Xiuju Liu, Kang Gan, Hong Liu, Xiaoqing Song, Tianjie Chen, Chenchen Liu
OBJECTVE: We aimed to investigate the cytotoxicity and antibacterial properties of nano-silver-coated polyetheretherketone (PEEK) produced through magnetron sputtering and provide a theoretical basis for its use in clinical applications. METHODS: The surfaces of PEEKs were coated with nano-silver at varying thicknesses (3, 6, 9, and 12nm) through magnetron sputtering technology. The resulting coated PEEK samples were classified into the following groups according to the thickness of the nano-silver coating: PEEK-3 (3nm), PEEK-6 (6nm), PEEK-9 (9nm), PEEK-12 (12nm), and PEEK control group...
July 19, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28733821/zero-valent-iron-particles-for-pcb-degradation-and-an-evaluation-of-their-effects-on-bacteria-plants-and-soil-organisms
#14
Alena Ševců, Yehia S El-Temsah, Jan Filip, Erik J Joner, Kateřina Bobčíková, Miroslav Černík
Two types of nano-scale zero-valent iron (nZVI-B prepared by borohydride reduction and nZVI-T produced by thermal reduction of iron oxide nanoparticles in H2) and a micro-scale ZVI (mZVI) were compared for PCB degradation efficiency in water and soil. In addition, the ecotoxicity of nZVI-B and nZVI-T particles in treated water and soil was evaluated on bacteria, plants, earthworms, and ostracods. All types of nZVI and mZVI were highly efficient in degradation of PCBs in water, but had little degradation effect on PCBs in soil...
July 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28732913/anti-bacterial-chitosan-zinc-phthalocyanine-fibers-supported-metallic-and-bimetallic-nanoparticles-for-the-removal-of-organic-pollutants
#15
Fayaz Ali, Sher Bahadar Khan, Tahseen Kamal, Yasir Anwar, Khalid A Alamry, Abdullah M Asiri
In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles. The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution. ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles. The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732725/isolation-of-polysaccharides-from-malva-aegyptiaca-and-evaluation-of-their-antioxidant-and-antibacterial-properties
#16
Nahed Fakhfakh, Ola Abdelhedi, Hamida Jdir, Moncef Nasri, Nacim Zouari
This study investigated the antioxidant and antibacterial properties of crude water-soluble polysaccharides extracted from the mallow (Malva aegyptiaca) by precipitation with cetylpyridinium chloride (P1) or ethanol (P2). The Polysaccharides fractions were characterized by FTIR spectroscopy, a monosaccharide composition, and antioxidant and antibacterial activities. P1 showed the highest total sugars (81.2%) and sulfated groups (2.9%) contents but with the lowest total proteins content (8.7%). The infrared spectroscopic spectra exhibited the typical bands and peak characteristic of polysaccharides...
July 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28732723/lipolytic-bacteria-use-as-bio-decontaminating-natural-in-the-water-purification-stations
#17
REVIEW
Lalia Hachemi, Zouaoui Benattouche, Mohammed Elnedhir Belgherras
Different samples were collected from polluted waters by fat materials in the Mascara region for the isolation of bacterial strains capable of degrading fat materials and used in the production of lipase by the fermentation method. A total of two strains (Pseudemonas sp et Streptococcus sp) were isolated at 37°C from the sample water rich slaughterhouses fat materials. Production of extracellular bacterial lipase was studied as a function of several inductors of lipid nature by the fermentation method. The enzyme activity reached a maximum value, in the presence of olive oil as inducer...
July 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28732554/microbial-ecology-and-process-technology-of-sourdough-fermentation
#18
Luc De Vuyst, Simon Van Kerrebroeck, Frédéric Leroy
From a microbiological perspective, sourdough is to be considered as a specific and stressful ecosystem, harboring yeasts and lactic acid bacteria (LAB), that is used for the production of baked goods. With respect to the metabolic impact of the sourdough microbiota, acidification (LAB), flavor formation (LAB and yeasts), and leavening (yeasts and heterofermentative LAB species) are most noticeable. Three distinct types of sourdough fermentation processes can be discerned based on the inocula applied, namely backslopped ones (type 1), those initiated with starter cultures (type 2), and those initiated with a starter culture followed by backslopping (type 3)...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28731479/marine-archaeal-dynamics-and-interactions-with-the-microbial-community-over-5-years-from-surface-to-seafloor
#19
Alma E Parada, Jed A Fuhrman
Marine archaea are critical contributors to global carbon and nitrogen redox cycles, but their temporal variability and microbial associations across the water column are poorly known. We evaluated seasonal variability of free living (0.2-1 μm size fraction) Thaumarchaea Marine Group I (MGI) and Euryarchaea Marine Group II (MGII) communities and their associations with the microbial community from surface to seafloor (890 m) over 5 years by 16S rRNA V4-V5 gene sequencing. MGI and MGII communities demonstrated distinct compositions at different depths, and seasonality at all depths...
July 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28731472/high-abundance-and-expression-of-transposases-in-bacteria-from-the-baltic-sea
#20
Theoden Vigil-Stenman, Karolina Ininbergs, Birgitta Bergman, Martin Ekman
Transposases are mobile genetic elements suggested to have an important role in bacterial genome plasticity and host adaptation but their transcriptional activity in natural bacterial communities is largely unexplored. Here we analyzed metagenomes and -transcriptomes of size fractionated (0.1-0.8, 0.8-3.0 and 3.0-200 μm) bacterial communities from the brackish Baltic Sea, and adjacent marine waters. The Baltic Sea transposase levels, up to 1.7% of bacterial genes and 2% of bacterial transcripts, were considerably higher than in marine waters and similar to levels reported for extreme environments...
July 21, 2017: ISME Journal
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