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https://www.readbyqxmd.com/read/28929962/-determination-of-in-vitro-susceptibilities-of-brucella-spp-strains-against-11-different-antibacterial-gents-isolated-from-blood-cultures
#1
Recep Keşli, Hüseyin Bilgin, Halim Yılmaz
Brucellosis is a worldwide zoonotic disease and still continuous to be a major public health problem. In this study, it was aimed to identify the Brucella strains to the species level isolated from blood cultures, and to determine the rate of antimicrobial susceptibility against eleven antibacterial agents. A total of 106 Brucella spp. strains were included in the study, which were isolated from blood cultures in University of Health Sciences, Konya Training and Research Hospital, Medical Microbiology Laboratory between January 2011 and June 2013...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28929961/-national-antimicrobial-resistance-surveillance-system-namrss-external-quality-assessment-studies-2011-2016
#2
Serap Süzük Yıldız, Hüsniye Şimşek, Nilay Çöplü, Zeynep Gülay
Establishment of sustainable and evidence-based surveillance systems are recommended for prevention of microbial resistance by the World Health Organization (WHO). As a necessity of these surveillance systems, participants are recommended to implement an external quality assessment (EQA) program. In this scope, National Antimicrobial Resistance Surveillance System (NARSS) has been established within the Public Health Institute of Turkey (PHIT) in our country since 2011. In the scope of this surveillance, NARSS EQA program has been implemented in a cycle per year and four isolates were sent to participants per cycle every year since 2011...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28927444/erratum-to-prevalence-of-methicillin-resistant-staphylococcus-aureus-carriage-on-admission-among-patients-attending-regional-hospitals-in-dar-es-salaam-tanzania
#3
Agricola Joachim, Sabrina J Moyo, Lillian Nkinda, Mtebe Majigo, Elia Mmbaga, Naboth Mbembati, Said Aboud, Eligius F Lyamuya
Following publication of the original article [1], author Elia Mmbaga pointed out that her name had been misspelt as Elia Mbaga.
September 19, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28924032/staphylococcus-aureus-strain-newman-d2c-contains-mutations-in-major-regulatory-pathways-that-cripple-its-pathogenesis
#4
William E Sause, Richard Copin, Aidan O'malley, Rita Chan, Brian J Morrow, Peter T Buckley, Jeffrey Fernandez, A Simon Lynch, Bo Shopsin, Victor J Torres
Staphylococcus aureus is a major human pathogen that imposes a great burden on the healthcare system. In the development of anti-staphylococcal modalities intended to reduce the burden of staphylococcal disease, it is imperative to select appropriate models of S. aureus strains when assessing the efficacy of novel agents. Here, using whole genome sequencing, we reveal that the commonly used strain Newman D2C from the American Type Culture Collection (ATCC) contains mutations that render the strain essentially avirulent...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28923605/microbial-pathogenicity-and-virulence-mediated-by-integrons-on-gram-positive-microorganisms
#5
REVIEW
Yanmei Li, Ling Yang, Jie Fu, Muxia Yan, Dingqiang Chen, Li Zhang
Gram-positive microorganisms are one of leading pathogenic microorganisms in public health, including several typical "Super Bugs" as methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae carbapenemase and vancomycin-resistant enterococci, which caused a increasement of infections, clinical failures and expenses. Regarded as a common genetic element responsible for horizontal gene transfer, integrons are widely distributed in various pathogens considered as a determinant in the acquisition and evolution of antibiotic resistance...
September 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28922613/the-etiology-of-community-acquired-pneumonia-among-children-under-5%C3%A2-years-of-age-in-mainland-china-2001-2015-a-systematic-review
#6
Guijun Ning, Xuxia Wang, Dan Wu, Zundong Yin, Yixing Li, Huaqing Wang, Weizhong Yang
BACKGROUND: The aim of this systematic review was to examine the etiology of community-acquired pneumonia (CAP) among Chinese children younger than 5 years and provide evidence for further cost-effectiveness analyses for vaccine development, diagnostic strategies and empirical treatments. METHODS: The literature review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Data were obtained by searching PubMed, Embase, Web-of Science, and the Chinese databases Wanfang Data and China National Knowledge Infrastructure...
September 18, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28922412/walker-occupancy-has-an-impact-on-changing-airborne-bacterial-communities-in-an-underground-pedestrian-space-as-small-dust-particles-increased-with-raising-both-temperature-and-humidity
#7
Torahiko Okubo, Takako Osaki, Eriko Nozaki, Akira Uemura, Kouhei Sakai, Mizue Matushita, Junji Matsuo, Shinji Nakamura, Shigeru Kamiya, Hiroyuki Yamaguchi
Although human occupancy is a source of airborne bacteria, the role of walkers on bacterial communities in built environments is poorly understood. Therefore, we visualized the impact of walker occupancy combined with other factors (temperature, humidity, atmospheric pressure, dust particles) on airborne bacterial features in the Sapporo underground pedestrian space in Sapporo, Japan. Air samples (n = 18; 4,800L/each sample) were collected at 8:00 h to 20:00 h on 3 days (regular sampling) and at early morning / late night (5:50 h to 7:50 h / 22:15 h to 24:45 h) on a day (baseline sampling), and the number of CFUs (colony forming units) OTUs (operational taxonomic units) and other factors were determined...
2017: PloS One
https://www.readbyqxmd.com/read/28919333/status-of-the-effectiveness-of-contact-lens-disinfectants-in-malaysia-against-keratitis-causing-pathogens
#8
Farhat Abjani, Naveed Ahmed Khan, Suk Yul Jung, Ruqaiyyah Siddiqui
The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept(®), AO SEPT PLUS, OPTI-FREE(®) pure moist(®), Renu(®) fresh™, FreshKon(®) CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT)...
September 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28915926/antimicrobial-resistance-patterns-of-staphylococcus-species-isolated-from-cats-presented-at-a-veterinary-academic-hospital-in-south-africa
#9
Daniel Nenene Qekwana, Dikeledi Sebola, James Wabwire Oguttu, Agricola Odoi
BACKGROUND: Antimicrobial resistance is becoming increasingly important in both human and veterinary medicine. This study investigated the proportion of antimicrobial resistant samples and resistance patterns of Staphylococcus isolates from cats presented at a veterinary teaching hospital in South Africa. Records of 216 samples from cats that were submitted to the bacteriology laboratory of the University of Pretoria academic veterinary hospital between 2007 and 2012 were evaluated. Isolates were subjected to antimicrobial susceptibility testing against a panel of 15 drugs using the disc diffusion method...
September 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28911341/rapid-detection-of-staphylococcus-aureus-and-related-species-isolated-from-food-environment-cosmetics-a-medical-device-and-clinical-samples-using-the-vitek-ms-microbial-identification-system
#10
Irshad M Sulaiman, Pratik Banerjee, Ying-Hsin Hsieh, Nancy Miranda, Steven Simpson, Khalil Kerdahi
Staphylococcus spp. is considered as one of the most common human-pathogenic bacteria, causing illnesses ranging from nonthreatening skin infections to lethal diseases, including sepsis, pneumonia, bloodstream infections, and food poisoning. The emergence of methicillin-resistant Staphylococcus aureus strains has increased morbidity and mortality and resulted in a major healthcare burden worldwide. Single and multilocus sequence typing have been extensively used in the identification of Staphylococcus species...
September 15, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28902291/emergence-of-methicillin-resistant-staphylococcus-aureus-from-clonal-complex-398-with-no-livestock-association-in-brazil
#11
Egidio Domingos André, Renata Freire Alves Pereira, Robert Eugene Snyder, Thamiris Santana Machado, Lialyz Soares Pereira André, Claudete Aparecida Araújo Cardoso, Fábio Aguiar-Alves
CC398 is a livestock-associated Staphylococcus aureus. However, it has also been isolated from humans with no previous contact with livestock. A surveillance of methicillin-resistant S. aureus colonisation among children attending public day care centres and hospitals in Niterói and Rio de Janeiro, Brazil, between 2011 and 2013, resulted in the isolation of six cases of CC398 from individuals with no previous exposure to livestock. These isolates showed a high frequency of the erm(C) gene (4/6, 66.7%) with induced resistance to clindamycin, and a relatively high frequency of SEs and lukS/lukF genes...
September 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28894731/prevalence-of-genotypes-that-determine-resistance-of-staphylococci-to-macrolides-and-lincosamides-in-serbia
#12
Milena Mišić, Jelena Čukić, Dejan Vidanović, Milanko Šekler, Sanja Matić, Mihailo Vukašinović, Dejan Baskić
Macrolides, lincosamides, and streptogramins (MLS) resistance genes are responsible for resistance to these antibiotics in Staphylococcus infections. The purpose of the study was to analyze the distribution of the MLS resistance genes in community- and hospital-acquired Staphylococcus isolates. The MLS resistance phenotypes [constitutive resistance to macrolide-lincosamide-streptogramin B (cMLSb), inducible resistance to macrolide-lincosamide-streptogramin B (iMLSb), resistance to macrolide/macrolide-streptogramin B (M/MSb), and resistance to lincosamide-streptogramin A/streptogramin B (LSa/b)] were determined by double-disc diffusion method...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28891454/current-technology-in-the-discovery-and-development-of-novel-antibacterials
#13
Pooi Yin Chung
Bacterial resistance to antibiotics is one of the most serious challenge to global public health. The introduction of new antibiotics in clinical settings, i.e. agents that belong to a new class of antibacterials, act on new targets or has a novel mechanisms of action, may not be sufficient to cope with the emergence of multidrug-resistant pathogens such as Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii and Escherichia coli, which are increasingly prevalent in healthcare settings in Europe, the USA and Asia...
September 10, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/28889756/occurrence-of-vancomycin-resistant-staphylococcus-aureus-in-the-oral-cavity-of-patients-with-dental-caries
#14
Sajith Vellappally, Darshan Devang Divakar, Abdulaziz Abdullah Al Kheraif, Ravikumar Ramakrishnaiah, Amer Alqahtani, M H N Dalati, Sukumaran Anil, Aftab Ahmed Khan, P R Harikrishna Varma
Oral streptococci are the major group of microbes isolated from oral microflora. They represent frequent pathogens of infective endocarditis (IE), and it is assumed that in most of the cases oral streptococci are acquired via mucosa layer of oral cavity. Staphylococcus aureus is also frequently isolated from IE as it accounts for 20%-30% of all cases. Vancomycin has been the most reliable therapeutic agent against infections caused by methicillin-resistant S. aureus (MRSA). The main objective of this study was to examine the occurrence of S...
September 11, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28887291/molecular-epidemiology-and-antimicrobial-susceptibility-of-methicillin-resistant-staphylococcus-aureus-isolates-in-xiangyang-china
#15
Yi-Tong Tang, Rui Cao, Na Xiao, Zhi-Shan Li, Rong Wang, Jiu-Ming Zou, Jiao Pei
OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) is a public health problem worldwide. This study aimed to investigate the antimicrobial susceptibilities and molecular epidemiological characteristics of MRSA strains in Xiangyang, China, during 2012-2014. METHODS: Eighty non-duplicate S. aureus isolates from clinical specimens were collected from four tertiary hospitals. MRSA strains were identified and tested for antibacterial susceptibility. Staphylococcus cassette chromosome mec (SCCmec) typing, Pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST) and spa typing were performed to explore molecular characteristics...
September 5, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28874470/inhibition-of-the-atp-synthase-eliminates-the-intrinsic-resistance-of-staphylococcus-aureus-towards-polymyxins
#16
Martin Vestergaard, Katrine Nøhr-Meldgaard, Martin Saxtorph Bojer, Christina Krogsgård Nielsen, Rikke Louise Meyer, Christoph Slavetinsky, Andreas Peschel, Hanne Ingmer
Staphylococcus aureus is intrinsically resistant to polymyxins (polymyxin B and colistin), an important class of cationic antimicrobial peptides used in treatment of Gram-negative bacterial infections. To understand the mechanisms underlying intrinsic polymyxin resistance in S. aureus, we screened the Nebraska Transposon Mutant Library established in S. aureus strain JE2 for increased susceptibility to polymyxin B. Nineteen mutants displayed at least 2-fold reductions in MIC, while the greatest reductions (8-fold) were observed for mutants with inactivation of either graS, graR, vraF, or vraG or the subunits of the ATP synthase (atpA, atpB, atpG, or atpH), which during respiration is the main source of energy...
September 5, 2017: MBio
https://www.readbyqxmd.com/read/28873428/evaluation-of-robenidine-analog-ncl195-as-a-novel-broad-spectrum-antibacterial-agent
#17
Abiodun D Ogunniyi, Manouchehr Khazandi, Andrew J Stevens, Sarah K Sims, Stephen W Page, Sanjay Garg, Henrietta Venter, Andrew Powell, Karen White, Kiro R Petrovski, Geraldine Laven-Law, Eliane G Tótoli, Hérida R Salgado, Hongfei Pi, Geoffrey W Coombs, Dean L Shinabarger, John D Turnidge, James C Paton, Adam McCluskey, Darren J Trott
The spread of multidrug resistance among bacterial pathogens poses a serious threat to public health worldwide. Recent approaches towards combating antimicrobial resistance include repurposing old compounds with known safety and development pathways as new antibacterial classes with novel mechanisms of action. Here we show that an analog of the anticoccidial drug robenidine (4,6-bis(2-((E)-4-methylbenzylidene)hydrazinyl)pyrimidin-2-amine; NCL195) displays potent bactericidal activity against Streptococcus pneumoniae and Staphylococcus aureus by disrupting the cell membrane potential...
2017: PloS One
https://www.readbyqxmd.com/read/28865851/prevalence-antimicrobial-susceptibility-and-molecular-characterization-of-staphylococcus-aureus-isolated-from-dairy-herds-in-northern-china
#18
Huimin Liu, Songli Li, Lu Meng, Lei Dong, Shengguo Zhao, Xinyi Lan, Jiaqi Wang, Nan Zheng
Staphylococcus aureus is one of the main pathogens involved in dairy cow mastitis. Monitoring of antibiotic use would prove useful to assess the risk of Staph. aureus in raw milk. The objective of this work was to investigate the prevalence of Staph. aureus strains isolated from raw milk in northern China, and to characterize antimicrobial susceptibility of these strains and their key virulence genes. In total, 195 raw milk samples were collected from 195 dairy farms located in 4 cities of northern China from May to September 2015...
August 30, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28859126/development-of-a-real-time-pcr-for-detection-of-staphylococcus-pseudintermedius-using-a-novel-automated-comparison-of-whole-genome-sequences
#19
Koen M Verstappen, Loes Huijbregts, Mirlin Spaninks, Jaap A Wagenaar, Ad C Fluit, Birgitta Duim
Staphylococcus pseudintermedius is an opportunistic pathogen in dogs and cats and occasionally causes infections in humans. S. pseudintermedius is often resistant to multiple classes of antimicrobials. It requires a reliable detection so that it is not misidentified as S. aureus. Phenotypic and currently-used molecular-based diagnostic assays lack specificity or are labour-intensive using multiplex PCR or nucleic acid sequencing. The aim of this study was to identify a specific target for real-time PCR by comparing whole genome sequences of S...
2017: PloS One
https://www.readbyqxmd.com/read/28854219/molecular-characterization-of-vancomycin-resistant-staphylococcus-aureus-strains-isolated-from-clinical-samples-a-three-year-study-in-tehran-iran
#20
Marjan Shekarabi, Bahareh Hajikhani, Alireza Salimi Chirani, Maryam Fazeli, Mehdi Goudarzi
INTRODUCTION: Emergence of vancomycin-intermediate Staphylococcus aureus (VISA) and vancomycin-resistant S. aureus (VRSA) strains has led to great concern in global public health in both developing and developed countries. This study investigated distribution and molecular characterization of VRSA strains in Tehran's hospitals using a combination of molecular typing methods. MATERIALS AND METHODS: A total of 1789 S. aureus isolates obtained between 2014 and 2017 and were characterized using antibiogram, SCCmec typing, spa typing, and multilocus-sequence typing...
2017: PloS One
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