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https://www.readbyqxmd.com/read/29209869/correction-to-effect-of-incubation-duration-growth-temperature-and-abiotic-surface-type-on-cell-surface-properties-adhesion-and-pathogenicity-of-biofilm-detached-staphylococcus-aureus-cells
#1
Simon Oussama Khelissa, Charafeddine Jama, Marwan Abdallah, Rabah Boukherroub, Christine Faille, Nour-Eddine Chihib
Following publication of the original article (Khelissa et al. 2017), the authors reported that the legend for Fig. 3 contained an error. Instead of "Adhesion of planktonic and biofilm-detached Staphylococcus aureus cells on stainless steel and polycarbonate. Cell cultures were grown at 20, 30 and 37 °C, during 24 h (white square) and 48 h (white square). Planktonic cells adhesion on stainless steel (a) and polycarbonate (b). Adhesion of stainless steel-biofilm-detached-cells on stainless steel 24 (c) and polycarbonate-biofilm-detached-cells on polycarbonate (d)", the legend should read "Adhesion of planktonic and biofilm-detached Staphylococcus aureus cells on stainless steel and polycarbonate...
December 5, 2017: AMB Express
https://www.readbyqxmd.com/read/29204285/molecular-characterization-of-acinetobacter-baumannii-isolated-from-iraqi-hospital-environment
#2
I M S Al-Kadmy, A N M Ali, I M A Salman, S S Khazaal
Healthcare-associated items are a common source of acquired infections, and hospital-acquired infections cause significant mortality and morbidity worldwide. Acinetobacter baumannii is the most prevalent infection-causing organism in the hospital environment. Hospital articles and objects are the main sources of infection with the ability to transmit some of the pathogenic microorganisms such as A. baumannii, which is considered a serious problem in therapeutic treatments. In the current study, we isolated A...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204221/antibiotic-resistance-patterns-and-virulence-determinants-of-different-sccmec-and-pulsotypes-of-staphylococcus-aureus-isolated-from-a-major-hospital-in-ilam-iran
#3
REVIEW
Mehdi Abbasi, Majid BaseriSalehi, Nima Bahador, Morovat Taherikalani
Aims & Objectives: The aim of this study is to evaluate genetic relatedness, antibiotic resistance pattern, and virulence characteristics of different types of S. aureus isolated from air, surfaces, staff, and patients in a Public hospital in Ilam. Methods & Materials: A total of 88 of 140 staphylococci identified as S. aureus by conventional and molecular methods were used in this study. Isolate samples were obtained from surfaces, staff, patients, and hospital indoor air...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29202045/adaptation-in-a-fibronectin-binding-autolysin-of-staphylococcus-saprophyticus
#4
Tatum D Mortimer, Douglas S Annis, Mary B O'Neill, Lindsey L Bohr, Tracy M Smith, Hendrik N Poinar, Deane F Mosher, Caitlin S Pepperell
Human-pathogenic bacteria are found in a variety of niches, including free-living, zoonotic, and microbiome environments. Identifying bacterial adaptations that enable invasive disease is an important means of gaining insight into the molecular basis of pathogenesis and understanding pathogen emergence. Staphylococcus saprophyticus, a leading cause of urinary tract infections, can be found in the environment, food, animals, and the human microbiome. We identified a selective sweep in the gene encoding the Aas adhesin, a key virulence factor that binds host fibronectin...
November 2017: MSphere
https://www.readbyqxmd.com/read/29197673/after-the-deluge-mining-staphylococcus-aureus-genomic-data-for-clinical-associations-and-host-pathogen-interactions
#5
REVIEW
Richard Copin, Bo Shopsin, Victor J Torres
The genome of Staphylococcus aureus has rapidly become one the most frequently sequenced among bacteria, with more than 40000 genome sequences uploaded to public databases. Computational resources required for analysis and quality assessment have lagged behind accumulation of sequence data. Improved analytic pipelines, in combination with the development of customized S. aureus reference databases, can be used to inform S. aureus biology and potentially predict clinical outcome. Here, we review the currently available data about S...
November 30, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29193746/dual-functional-dextran-peg-hydrogel-as-an-antimicrobial-biomedical-material
#6
Lei Wang, Xin Li, Tianyu Sun, Yung-Hao Tsou, Hong Chen, Xiaoyang Xu
Microbial infections continually present a major worldwide public healthcare threat, particularly in instances of impaired wound healing and biomedical implant fouling. The development of new materials with the desired antimicrobial property to avoid and treat wound infection is urgently needed in wound care management. This study reports a novel dual-functional biodegradable dextran-poly(ethylene glycol) (PEG) hydrogel covalently conjugated with antibacterial Polymyxin B and Vancomycin (Vanco). The hydrogel is designed as a specialized wound dressing that eradicates existing bacteria and inhibits further bacteria growth, while, ameliorating the side effects of antibiotics and accelerating tissue repair and regeneration...
November 29, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29188779/mrsa-prevalence-and-susceptibility-pattern-in-health-care-setups-of-karachi
#7
Tuba Siddiqui, Iyad Naeem Muhammad, Muhammad Naseem Khan, Shazia Naz, Lubna Bashir, Nosheen Sarosh, Rida Masood, Aatka Ali, Sakina Fatima, Talat Naqvi
This assessment aims to determine the prevalence of methicillin resistance and multidrug resistance (MDR) among the clinical isolates of Staphylococcus aureus and antimicrobial susceptibility profile of methicillin resistant Staphylococcus aureus (MRSA) to the frequently prescribed antibiotics in Karachi. Isolates of MRSA, recovered from various clinical samples were included in this prospective, cross-sectional study from Jan 2015 to June 2017. Agar diffusion method was employed according to the protocols of Clinical Laboratory Standards Institute...
November 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29187146/determination-of-antimicrobial-susceptibility-patterns-in-staphylococcus-aureus-strains-recovered-from-patients-at-two-main-health-facilities-in-kabul-afghanistan
#8
Haji Mohammad Naimi, Hamidullah Rasekh, Ahmad Zia Noori, Mohammad Aman Bahaduri
BACKGROUND: Staphylococcus aureus (S. aureus) is a major pathogen implicated in skin and soft tissue infections, abscess in deep organs, toxin mediated diseases, respiratory tract infections, urinary tract infections, post-surgical wound infections, meningitis and many other diseases. Irresponsible and over use of antibiotics has led to an increased presence of multidrug resistant organisms and especially methicillin resistant Staphylococcus aureus (MRSA) as a major public health concern in Afghanistan...
November 29, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29186107/mucocutaneous-il-17-immunity-in-mice-and-humans-host-defense-vs-excessive-inflammation
#9
REVIEW
J Li, J-L Casanova, A Puel
Interleukin (IL)-17A is a pro-inflammatory cytokine in mice and humans. It is recognized as a key factor for the protection of mice against various pathogens, but it also underlies pathogenic inflammatory responses in numerous mouse models. The inborn errors of IL-17A- and IL-17F-mediated immunity identified in humans in the last decade have revealed that IL-17A and IL-17F are key players in mucocutaneous immunity to Candida albicans, and, to a lesser extent, Staphylococcus aureus. By contrast, there is currently no genetic evidence for a causal link between excess of IL-17 and autoimmunity, autoinflammation, or allergy in humans...
November 29, 2017: Mucosal Immunology
https://www.readbyqxmd.com/read/29184366/polymerase-chain-reaction-detection-of-genes-responsible-for-multiple-antibiotic-resistance-staphylococcus-aureus-isolated-from-food-of-animal-origin-in-egypt
#10
Fawzy R El Seedy, A A Samy, Hala S H Salam, Eman A Khairy, Aya A Koraney
Aim: The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt. Materials and Methods: A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes...
October 2017: Veterinary World
https://www.readbyqxmd.com/read/29184121/isolation-and-structure-elucidation-of-lipopeptide-antibiotic-taromycin-b-from-the-activated-taromycin-biosynthetic-gene-cluster
#11
Kirk A Reynolds, Hanna Luhavaya, Jie Li, Samira Dahesh, Victor Nizet, Kazuya Yamanaka, Bradley S Moore
In the ongoing effort to unlock the chemical potential of marine bacteria, genetic engineering of biosynthetic gene clusters (BGCs) is increasingly used to awake or improve expression of biosynthetic genes that may lead to discovery of novel bioactive natural products. Previously, we reported the successful capture, engineering and heterologous expression of an orphan BGC from the marine actinomycete Saccharomonospora sp. CNQ-490, which resulted in the isolation of the novel lipopeptide antibiotic taromycin A...
November 29, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/29178873/prevalence-and-molecular-characterization-of-staphylococcus-aureus-isolated-from-goats-in-chongqing-china
#12
Zuoyong Zhou, Mengsi Zhang, Hexian Li, Haoyue Yang, Xiaoxia Li, Xinyue Song, Zhiying Wang
BACKGROUND: Staphylococcus aureus is an important zoonotic pathogen which not only causes significant economic loss in livestock production but also poses a potential threat to public health. Compared with bovine and swine, the information on the colonization of S. aureus in goats is very limited. To understand the prevalence and characteristics of S. aureus in goats, we used the nasal swabs collected from apparently healthy goats to isolate S. aureus, and tested their antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing (MLST)...
November 25, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29169222/metagenomic-approach-to-identifying-foodborne-pathogens-on-chinese-cabbage
#13
Daeho Kim, Sanghyun Hong, You-Tae Kim, Sangryeol Ryu, Hyeun Bum Kim, Ju-Hoon Lee
Foodborne illness represents a major threat to public health frequently attributed to pathogenic microorganisms on fresh produce. Recurrent outbreaks often come from vegetables that are grown close to or within the ground. Therefore, the first step to understanding the public health risk of microorganisms on fresh vegetables is to identify and describe microbial communities. We investigated phyllospheres on Chinese cabbage (Brassica rapa subsp. pekinensis, n=54). 16S rRNA gene amplicon sequencing targeting the V5-V6 region of 16S rRNA genes was conducted by employing the Illumina MiSeq system...
November 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29166860/msaabcr-operon-is-involved-in-persister-cell-formation-in-staphylococcus-aureus
#14
Gyan S Sahukhal, Shanti Pandey, Mohamed O Elasri
BACKGROUND: Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evidences of the presence of persister cells in recalcitrant infections underscores the great urgency to unravel the mechanism by which these cells develop. Previously, we characterized msaABCR operon that plays roles in regulation of virulence, biofilm development and antibiotic resistance...
November 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29163457/the-agr-quorum-sensing-system-represses-persister-formation-through-regulation-of-phenol-soluble-modulins-in-staphylococcus-aureus
#15
Tao Xu, Xu-Yang Wang, Peng Cui, Yu-Meng Zhang, Wen-Hong Zhang, Ying Zhang
The opportunistic pathogen Staphylococcus aureus has become an increasing threat to public health. While the Agr quorum sensing (QS) system is a master regulator of S. aureus virulence, its dysfunction has been frequently reported to promote bacteremia and mortality in clinical infections. Here we show that the Agr system is involved in persister formation in S. aureus. Mutation of either agrCA or agrD but not RNAIII resulted in increased persister formation of stationary phase cultures. RNA-seq analysis showed that in stationary phase AgrCA/AgrD and RNAIII mutants showed consistent up-regulation of virulence associated genes (lip and splE, etc...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29157372/prevalence-of-antimicrobial-resistance-from-bacterial-culture-and-susceptibility-records-from-horse-samples-in-south-africa
#16
John K Chipangura, Thireshni Chetty, Marcia Kgoete, Vinny Naidoo
The continuous increase in prevalence of antimicrobial resistant bacteria presents a significant public health problem and is an indicator that antimicrobial prudent usage guidelines are not being followed, especially in developing countries. Despite trends being available from numerous countries, there is little published for South Africa. This study was aimed at estimating the prevalence and trends of antimicrobial resistance from bacterial isolates from equine clinical samples submitted for culture and susceptibility testing to the veterinary bacteriology laboratory of the University of Pretoria...
December 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29152103/myxinidin2-and-myxinidin3-suppress-inflammatory-responses-through-stat3-and-mapks-to-promote-wound-healing
#17
Hyo Mi Han, Sujin Ko, Min-Ju Cheong, Jeong Kyu Bang, Chang Ho Seo, Tudor Luchian, Yoonkyung Park
Skin wounds are continuously exposed to bacteria and can easily become infected. Infected wounds require antibiotic treatment, and infections caused by drug-resistant bacteria are an important public health problem. Antimicrobial peptides have broad-spectrum antibacterial activity, induce little or no drug resistance and may be suitable for treating skin infections caused by drug-resistant bacteria. We previously reported the design and function of myxinidin and myxinidin analogues. Here we showed that myxinidin2 and myxinidin3 exhibit antimicrobial and anti-biofilm activities against antibiotic-resistant Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa in high salt environments and in gelatin...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29145465/hospital-associated-methicillin-resistant-staphylococcus-aureus-a-cross-sectional-analysis-of-risk-factors-in-south-african-tertiary-public-hospitals
#18
Liliwe L Shuping, Lazarus Kuonza, Alfred Musekiwa, Samantha Iyaloo, Olga Perovic
INTRODUCTION: Hospital-associated methicillin-resistant S. aureus (HA-MRSA) remains a significant cause of morbidity and mortality worldwide. We conducted a study to determine risk factors for HA-MRSA in order to inform control strategies in South Africa. METHODS: We used surveillance data collected from five tertiary hospitals in Gauteng and Western Cape provinces during 2014 for analysis. A case of HA-MRSA was defined as isolation of MRSA from a blood culture 48 hours after admission and/or if the patient was hospitalised in the six months prior to the current culture...
2017: PloS One
https://www.readbyqxmd.com/read/29138481/quadoctomycin-a-48-membered-macrolide-antibiotic-from-streptomyces-sp-mm168-141f8
#19
Ryuichi Sawa, Yumiko Kubota, Maya Umekita, Masaki Hatano, Chigusa Hayashi, Masayuki Igarashi
Drug-resistant bacteria are still emerging, and screening of new skeletal antibiotics is important. During our continuous screening for antimicrobial agents, we discovered a new antimicrobial, named quadoctomycin, from solid culture of Streptomyces sp. MM168-141F8. The substance was purified by solvent extraction, silica gel chromatography and HPLC. Structural elucidation of quadoctomycin was performed by MS and NMR analyses and chemical degradation. Quadoctomycin possesses a 48-membered polyol macrolide skeleton in which an α-D-mannoside is connected to C-22 by an O-glycosidic linkage...
November 15, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/29138082/synthesis-of-chitosan-alginate-microspheres-with-high-antimicrobial-and-antibiofilm-activity-against-multi-drug-resistant-microbial-pathogens
#20
Rajagopalan Thaya, Baskaralingam Vaseeharan, Jeyachandran Sivakamavalli, Arokiadhas Iswarya, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Mohammed N Al-Anbr, Jamal M Khaled, Giovanni Benelli
The successful treatment of multi-drug resistant microbial pathogens represents a major challenge for public health management. Here, chitosan-alginate (CS/ALG) microspheres with narrow size distribution were fabricated by ionically cross linking method using Ca(2+) ions as agents for polymer solidification. The physicochemical properties of CS/ALG microspheres, such as surface morphology and size, were studied by SEM. The functional group interactions were confirmed by Fourier transform infrared (FTIR) spectroscopy...
November 11, 2017: Microbial Pathogenesis
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