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https://www.readbyqxmd.com/read/29693603/comparison-of-staphylococcus-phage-k-with-close-phage-relatives-commonly-employed-in-phage-therapeutics
#1
Jude Ajuebor, Colin Buttimer, Sara Arroyo-Moreno, Nina Chanishvili, Emma M Gabriel, Jim O'Mahony, Olivia McAuliffe, Horst Neve, Charles Franz, Aidan Coffey
The increase in antibiotic resistance in pathogenic bacteria is a public health danger requiring alternative treatment options, and this has led to renewed interest in phage therapy. In this respect, we describe the distinct host ranges of Staphylococcus phage K, and two other K-like phages against 23 isolates, including 21 methicillin-resistant S. aureus (MRSA) representative sequence types representing the Irish National MRSA Reference Laboratory collection. The two K-like phages were isolated from the Fersisi therapeutic phage mix from the Tbilisi Eliava Institute, and were designated B1 (vB_SauM_B1) and JA1 (vB_SauM_JA1)...
April 25, 2018: Antibiotics
https://www.readbyqxmd.com/read/29693373/fabrication-of-self-healing-hydrogel-with-on-demand-antimicrobial-activity-and-sustained-biomolecules-release-for-infected-skin-regeneration
#2
Ran Tian, Xinyu Qiu, Pingyun Yuan, Kai Lei, Lin Wang, Yongkang Bai, Shiyu Liu, Xin Chen
Microbial infection has been considered as one of the most critical challenges in bioengineering applications especially in tissue regeneration, which engender severe threat to public health. Herein, a hydrogel performing properties of rapid self-healing, on-demand antibiosis and controlled cargo release were fabricated by a simple assembly of Fe complex as crosslinker and hyaluronic acid as gel network. This hydrogel is able to locally degrade and release Fe3+ to kill bacteria as needed, due to the hyaluronidase excreted by surrounding bacteria, resulting in efficient antibacterial activity to different types of bacteria...
April 25, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29690529/aerobic-mesophilic-coliform-escherichia-coli-and-staphylococcus-aureus-counts-of-raw-meat-from-the-formal-and-informal-meat-sectors-in-south-africa
#3
Ishmael Festus Jaja, Ezekiel Green, Voster Muchenje
Foodborne disease (FBD) is a global public health concern, and foods from animal sources have been associated with outbreaks of food-related illness. In this study, animal carcasses from the two abattoirs (HT1 and HT2) in the formal meat sector (FMS) and slaughter points in the informal meat sector (INMS) were examined at two stages of slaughter (before washing and after washing) for aerobic colony counts (ACC) and total viable count (TCC), as well as Escherichia coli and Staphylococcus aureus count. At each stage, carcasses were sampled by swabbing at the neck, brisket, flank, and rump...
April 21, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29686670/alum-toll-like-receptor-7-adjuvant-enhances-the-expansion-of-memory-b-cell-compartment-within-the-draining-lymph-node
#4
Hoa Thi My Vo, Barbara Christiane Baudner, Stefano Sammicheli, Matteo Iannacone, Ugo D'Oro, Diego Piccioli
Vaccination is one of the most cost-effective health interventions and, with the exception of water sanitization, no other action has had such a major effect in mortality reduction. Combined with other approaches, such as clean water, better hygiene, and health education, vaccination contributed to prevent millions of cases of deaths among children under 5 years of age. New or improved vaccines are needed to fight some vaccine-preventable diseases that are still a threat for the public health globally, as reported also in the Global Vaccine Action Plan (GVAP) endorsed by the World Health Assembly in 2012...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29686148/increasing-rates-of-penicillin-sensitivity-in-staphylococcus-aureus
#5
EDITORIAL
Guillaume Butler-Laporte, Todd C Lee, Matthew P Cheng
Recent publications have reported on the rise of penicillin sensitive staphylococcus aureus (PSSA).….
April 23, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29675779/antibiotic-resistance-and-molecular-characteristics-of-staphylococcus-aureus-isolated-from-backyard-raised-pigs-and-pig-workers
#6
Asabe Halimat Momoh, Jacob K P Kwaga, Mohammed Bello, Anthony K B Sackey, Anders Rhod Larsen
Staphylococcus aureus is a commensal and pathogenic bacterium with impact on public health and livestock industry. The study investigated nasal carriage, antibiotic resistance, and molecular characterization of S. aureus in pigs and pig workers. Nasal swabs from 300 backyard-raised pigs and 101 pig workers were used for the study. Resulting isolates were confirmed using MALDI-TOF MS, tested for antibiotic resistance, and three different multiplex PCRs were used to detect enterotoxin, mecA, spaA, scn, and pvl genes...
April 19, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29672171/prevalence-of-enterotoxin-genes-in-staphylococcus-aureus-isolates-from-pork-production
#7
Yang Zhang, Yage Wang, Rujian Cai, Lei Shi, Chunling Li, He Yan
In this study, 130 Staphylococcus aureus isolates from samples associated with pork production were tested for prevalence of 18 staphylococcal enterotoxin (SE) genes. Approximately 94.6% (123/130) of isolates from different stages of pork production harbored one or more SE genes forming 37 different enterotoxin gene profiles. Seb was present in 60.0% of the S. aureus isolates, the highest among the genes tested. The genes, sed, sej, seo, sep, ser, and seu, were not found. The five classical SE genes (including sea, seb, sec, sed, see) had lower prevalence than the egc gene cluster (seg, sei, sem, sen, seo, or seu)...
April 19, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29669512/a-systematic-review-of-transmission-dynamic-studies-of-methicillin-resistant-staphylococcus-aureus-in-non-hospital-residential-facilities
#8
Kin On Kwok, Jonathan M Read, Arthur Tang, Hong Chen, Steven Riley, Kai Man Kam
BACKGROUND: Non-hospital residential facilities are important reservoirs for MRSA transmission. However, conclusions and public health implications drawn from the many mathematical models depicting nosocomial MRSA transmission may not be applicable to these settings. Therefore, we reviewed the MRSA transmission dynamics studies in defined non-hospital residential facilities to: (1) provide an overview of basic epidemiology which has been addressed; (2) identify future research direction; and (3) improve future model implementation...
April 18, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29662467/multilocus-sequence-typing-and-virulence-associated-gene-profile-analysis-of-staphylococcus-aureus-isolates-from-retail-ready-to-eat-food-in-china
#9
Xiaojuan Yang, Shubo Yu, Qingping Wu, Jumei Zhang, Shi Wu, Dongli Rong
The aim of this study was to characterize the subtypes and virulence profiles of 69 Staphylococcus aureus isolates obtained from retail ready-to-eat food in China. The isolates were analyzed using multilocus sequence typing (MLST) and polymerase chain reaction (PCR) analysis of important virulence factor genes, including the staphylococcal enterotoxin (SE) genes ( sea , seb , sec , sed , see , seg , seh , sei , sej ), the exfoliative toxin genes ( eta and etb ), the toxic shock syndrome toxin-1 gene ( tst ), and the Panton-Valentine leucocidin-encoding gene ( pvl )...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29657423/prevalence-and-characterization-of-panton-valentine-leukocidin-positive-staphylococcus-aureus-in-bovine-milk-in-jabalpur-district-of-madhya-pradesh-india
#10
Neeraj Shrivastava, Varsha Sharma, Arpita Shrivastav, Anju Nayak, Ajay Kumar Rai
Aim: The study aimed to investigate the Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus in bovine milk due to its public health significance. Materials and Methods: A total of 400 milk samples of bovines taken from different dairy farms and outlets of Jabalpur were screened for the S. aureus and methicillin-resistant S. aureus (MRSA). The strains were tested for the PVL gene and antimicrobial sensitivity toward 10 different classes of antimicrobial agents...
March 2018: Veterinary World
https://www.readbyqxmd.com/read/29652493/reprogramming-probiotic-lactobacillus-reuteri-as-a-biosensor-for-staphylococcus-aureus-derived-aip-i-detection
#11
David Lubkowicz, Chun Loong Ho, In Young Hwang, Wen Shan Yew, Yung Seng Lee, Matthew Wook Chang
Gram-positive Staphylococcus aureus infection that results in pneumonia, urinary tract infection, and in severe cases, sepsis, has recently been classified as a serious threat to public health. Rapid and cost-effective detection of these infections are costly and time-consuming. Here, we present probiotic lactic acid bacteria engineered to detect autoinducer peptide-I (AIP-I), a quorum sensing molecule produced by Staphylococcus sp. during pathogenesis. We achieved this by adapting the well-characterized agr quorum sensing ( agrQS) from Staphylococcus aureus into Lactobacillus reuteri...
April 13, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29652195/a-meta-analysis-of-major-foodborne-pathogens-in-chinese-food-commodities-between-2006-and-2016
#12
Narayan Paudyal, Hang Pan, Xiayi Liao, Xian Zhang, Xiaoliang Li, Weihuan Fang, Min Yue
Prevalence of pathogenic bacteria in food commodities in China have been reported in numerous publications over time. However, the results are scattered and varied. To calculate a robust point estimate with a higher statistical power, we applied meta-analytic approach for investigating the prevalence of common foodborne pathogens in major food items in China. Data, on prevalence of bacteria in various food commodities were extracted and analyzed from 361 (132 English and 229 Chinese) publications. Prevalence of eight most frequently reported pathogens on six broad food categories was used for pooled and subgroup meta-analysis by DerSimonian-Laird method in random-effects model...
April 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29621629/genetic-characterization-of-staphylococcus-aureus-isolated-from-milk-and-nasal-samples-of-healthy-cows-in-tunisia-first-report-of-st97-t267-agri-sccmecv-mrsa-of-bovine-origin-in-tunisia
#13
Monia Khemiri, Mohamed Salah Abbassi, Natacha Couto, Riadh Mansouri, Salah Hammami, Constança Pomba
OBJECTIVES: This study aimed to screen for and characterize methicillin-susceptible S. aureus (MSSA) and MRSA in nasal swabs and milk from healthy cows from different regions in Tunisia. METHODS: One hundred and forty-one Staphylococcus spp. isolates were recovered from milk and nasal swabs of cows. S. aureus isolates were further characterized by determining their antibiotic susceptibilities, genes encoding antibiotic resistance and virulence factors, biofilm production, agr type and PFGE...
April 2, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29615254/variability-of-sccmec-elements-in-livestock-associated-cc398-mrsa
#14
Stefan Monecke, Peter Slickers, Darius Gawlik, Elke Müller, Annett Reissig, Antje Ruppelt-Lorz, Sonia Cortez de Jäckel, Andrea T Feßler, Martina Frank, Helmut Hotzel, Kristina Kadlec, Lutz Jatzwauk, Igor Loncaric, Stefan Schwarz, Katharina Schlotter, Alexander Thürmer, Sarah Wendlandt, Ralf Ehricht
The most common livestock-associated lineage of methicillin-resistant Staphylococcus aureus (MRSA) in Western Europe is currently clonal complex (CC) 398. CC398-MRSA spread extensively across livestock populations in several Western European countries, and livestock-derived CC398-MRSA strains can also be detected in humans. Based on their SCCmec elements, different CC398 strains can be distinguished. SCCmec elements of 100 veterinary and human CC398-MRSA isolates from Germany and Austria were examined using DNA microarray-based assays...
April 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29604297/demonstrating-success-in-reducing-adult-cardiac-surgical-site-infections-and-the-economic-impact-of-using-multidisciplinary-collaboration
#15
Lilian Chiwera, Neil Wigglesworth, Carol McCoskery, Gianluca Lucchese, William Newsholme
BACKGROUND: Cardiac surgical site infections (SSIs) have devastating consequences and present several challenges for patients and healthcare providers. Adult cardiac SSI surveillance commenced in 2009 at our hospitals, Guy's & St Thomas' NHS Foundation Trust, London, as a patient safety initiative amid reported increased incidence of SSIs. Before this time, infection incidence was unclear because data collection was not standardised. AIMS: Our aim was to standardise SSI data collection and establish baseline SSI rates to facilitate deployment of evidence based targeted interventions within clinical governance structures to improve quality, safety and efficiency in line with our organisational targets...
March 28, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29600576/meta-analysis-of-trials-comparing-cefazolin-to-anti-staphylococcal-penicillins-in-the-treatment-of-methicillin-sensitive-staphylococcus-aureus-bacteremia
#16
Joseph P Rindone, Chadwick K Mellen
AIMS: The objective of this study was to determine the effectiveness and safety of cefazolin versus anti-staphylococcal penicillin (ASP) in the treatment of methicillin-sensitive Staphylococcal aureus bacteremia (MSSA). METHODS: The data bases of PubMed, Embase, Cochrane Central were used to identify comparative trials of cefazolin versus ASP in MSSA bacteremia. Meta-analysis of included trials was performed to assess any differences regarding mortality, clinical cure, recurrence and withdrawal from adverse effects between groups...
March 30, 2018: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29599752/transcriptional-response-of-staphylococcus-aureus-to-sunlight-in-oxic-and-anoxic-conditions
#17
Jill S McClary, Alexandria B Boehm
The transcriptional response of Staphylococcus aureus strain Newman to sunlight exposure was investigated under both oxic and anoxic conditions using RNA sequencing to gain insight into potential mechanisms of inactivation. S. aureus is a pathogenic bacterium detected at recreational beaches which can cause gastrointestinal illness and skin infections, and is of increasing public health concern. To investigate the S. aureus photostress response in oligotrophic seawater, S. aureus cultures were suspended in seawater and exposed to full spectrum simulated sunlight...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29590259/individual-variation-is-the-key-to-the-development-of-a-vaccine-against-staphylococcus-aureus-a-comparative-study-between-mice-lineages
#18
D P Dos Santos, I P R Muniz, A F Queiroz, I S Pereira, M P A Souza, L J Lima, L R O Sousa, I S Ribeiro, M P L Galantini, L M Marques, T B Figueiredo, R A A da Silva
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages...
March 26, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29581051/febrile-illness-in-asia-gaps-in-epidemiology-diagnosis-management-for-informing-health-policy
#19
REVIEW
Poojan Shrestha, Tamalee Roberts, Anousin Homsana, Tin Ohn Myat, John A Crump, Yoel Lubell, Paul N Newton
BACKGROUND: There is increasing evidence on the aetiology and management of fevers in Asia, the importance of which has risen with declines in malaria incidence. AIMS: To conduct a narrative review of the epidemiology and management of fevers in South and South-East Asia and to highlight gaps in our knowledge that impair evidence-based health policy decisions. SOURCES: A narrative review of papers published since 2012 of developments in fever epidemiology, diagnosis and treatment in South and South-East Asia...
March 23, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29551999/design-synthesis-and-evaluation-of-branched-rrwqwr-based-peptides-as-antibacterial-agents-against-clinically-relevant-gram-positive-and-gram-negative-pathogens
#20
Sandra C Vega, Diana A Martínez, María Del S Chalá, Hernán A Vargas, Jaiver E Rosas
Multidrug resistance of pathogenic bacteria has become a public health crisis that requires the urgent design of new antibacterial drugs such as antimicrobial peptides (AMPs). Seeking to obtain new, lactoferricin B (LfcinB)-based synthetic peptides as viable early-stage candidates for future development as AMPs against clinically relevant bacteria, we designed, synthesized and screened three new cationic peptides derived from bovine LfcinB. These peptides contain at least one RRWQWR motif and differ by the copy number (monomeric, dimeric or tetrameric) and structure (linear or branched) of this motif...
2018: Frontiers in Microbiology
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