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Arannadia Barbosa Silva, Karen Medeiros Cardoso, Stefan Vilges de Oliveira, Raylene Medeiros Ferreira Costa, Geane Oliveira, Marinete Amorim, Leucio Câmara Alves, Maria Fernanda Melo Monteiro, Gilberto Salles Gazeta
In Brazil, active infections of Rickettsia spp. is confirmed in all regions, involving various species of ticks. During investigation of a new focus of Spotted Fever (SF) incidence in the state of Pernambuco, northeastern Brazil, tick species Rhipicephalus sanguineus sensu lato (Latreille), and Amblyomma pseudoconcolor were collected from one Canis familiaris and four Dasypus novemcinctus, respectively, and analized for the presence of rickettsial genes. Ten A. pseudoconcolor ticks (90.9%) were found to be infected with Rickettsia, whereas no evidence of Rickettsia spp...
March 12, 2018: Acta Tropica
Marina E Eremeeva, Lindsey M Weiner, Maria L Zambrano, Gregory A Dasch, Renjie Hu, Inger Vilcins, Martin B Castro, Denise L Bonilla, Kerry A Padgett
The rabbit tick, Haemaphysalis leporispalustris Packard, is known for its association with Rickettsia rickettsii as it harbors both virulent and avirulent strains of this pathogen. In this manuscript we report findings and preliminary characterization of a novel spotted fever group rickettsia (SFGR) in rabbit ticks from California, USA. Rickettsia sp. CA6269 (proposed "Candidatus Rickettsia lanei") is most related to known R. rickettsii isolates but belongs to its own well-supported branch different from those of all R...
March 1, 2018: Ticks and Tick-borne Diseases
Mohammad Bagher Ghavami, Habibeh Mirzadeh, Jamshid Mohammadi, Asghar Fazaeli
The human flea is an important ectoparasite causing serious public health problems worldwide. Planning and monitoring the control programs against this vector require the knowledge of population structure and vector competence. This study was carried out to identify molecular structure of internal transcribed spacer 1 (ITS1) of ribosomal gene and its capability in the survey of Pulex irritans populations as well as to investigate Rickettsia infection in these populations. Flea samples were collected via human baits from animal farms in two districts of Zanjan Province, northwest of Iran...
March 14, 2018: Parasitology Research
A S Varela-Stokes, S H Park, J V Stokes, N A Gavron, S I Lee, G M Moraru, S C Ricke
Our objective of this study was to explore the bacterial microbiome in fresh or fresh-frozen adult Amblyomma maculatum (Gulf Coast ticks) using extracts enriched for microbial DNA. We collected 100 questing adult A. maculatum, surface disinfected them, and extracted DNA from individual ticks collected the same day or after storage at -80 °C. Because only extracts with microbial DNA concentrations above 2 ng/μL were considered suitable for individual analysis, we expected fewer samples to meet these requirements...
March 1, 2018: Ticks and Tick-borne Diseases
Mengfei Wang, Dan Zhu, Jianfeng Dai, Zhengwei Zhong, Yi Zhang, Jingwen Wang
Ticks are important disease vectors as they transmit a variety of human and animal pathogens worldwide. Symbionts that co-evolved with ticks confer crucial benefits to their host in nutrition metabolism, fecundity and vector competence. Although over a hundred-tick species have been identified in China, general information of tick symbiosis is limited. Here, we visualized the tissue distribution of Coxiella sp. and Rickettsia sp. in lab reared Haemaphysalis longicornis and Rhipicephalus haemaphysaloides by fluorescent in situ hybridization...
March 9, 2018: Applied and Environmental Microbiology
Ιlias Chaligiannis, Isabel G Fernández de Mera, Anna Papa, Smaragda Sotiraki, José de la Fuente
Ticks are vectors for a variety of human and animal pathogens (bacteria, protozoa and viruses). In order to investigate the pathogens carried by ticks in Greece, a total of 179 adult ticks (114 female and 65 male) were collected from domestic animals (sheep, goats and dogs) from 14 prefectures of six regions of Greece. Among them, 40 were Dermacentor marginatus, 25 Haemaphysalis parva, 22 H. sulcata, one H. punctata, 13 Ixodes gibbosus, 77 Rhipicephalus sanguineus s.l. and one R. bursa. All ticks were tested for the presence of DNA of Anaplasma spp...
March 7, 2018: Experimental & Applied Acarology
Anna Michalik, Jacek Szwedo, Adam Stroiński, Dariusz Świerczewski, Teresa Szklarzewicz
In contrast to Cicadomorpha, in which numerous symbiotic bacteria have been identified and characterized, the symbionts of fulgoromorphans are poorly known. Here, we present the results of histological, ultrastructural, and molecular analyses of the symbiotic system of the planthopper Ommatidiotus dissimilis. Amplification, cloning, and sequencing of bacterial 16S RNA genes have revealed that O. dissimilis is host to five types of bacteria. Apart from bacteria Sulcia and Vidania, which are regarded as ancestral symbionts of Fulgoromorpha, three additional types of bacteria belonging to the genera Sodalis, Wolbachia, and Rickettsia have been detected...
March 7, 2018: Protoplasma
Bessie H Lockwood, Iga Stasiak, Madeleine A Pfaff, Christopher A Cleveland, Michael J Yabsley
The geographical distribution of Ixodes scapularis and Amblyomma maculatum ticks is poorly understood in Kentucky. We conducted a convenience survey of wildlife species (white-tailed deer (Odocoileus virginianus), elk (Cervus canadensis) and black bears (Ursus americanus)) for ticks from October 2015 to January 2017. We detected four tick species including Amblyomma americanum, Dermacentor albipictus, I. scapularis and A. maculatum. Although the former two tick species were previously known to be widely distributed in Kentucky, we also found that I...
March 1, 2018: Ticks and Tick-borne Diseases
Aurélie Morand, Emmanouil Angelakis, Mehdi Ben Chaabane, Philippe Parola, Didier Raoult, Philippe Gautret
BACKGROUND: Skin infections are among the leading causes of diseases in travelers. Diagnosing pathogens could be difficult. METHOD: We applied molecular assays for the diagnostic of a large collection of skin biopsies and swabs from travelers with suspected skin infections. All samples were tested by qPCR for Coxiella burnetti, Bartonella sp., Rickettsia sp., Borrelia sp., Ehrlichia sp., Tropheryma whipplei, Francisella tularensis, Mycobacteria sp., Staphylococcus aureus, Streptococcus pyogenes, Leishmania spp...
March 2, 2018: Travel Medicine and Infectious Disease
Tahseen Raza Hashmi, Salam Rita Devi, Naresh M Meshram, Ram Prasad
Endosymbionts are vital factor for arthropod ecology. The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a cryptic species complex composed of more than 34 putative species. Moreover to the primary endosymbiont Portiera aleyrodidarum , six secondary endosymbionts Cardinium , Arsenophonus , Rickettsia , Wolbachia, Hamiltonella and Fritschea are known in B . tabaci . Here, we tested four of the six secondary endosymbiont lineages (excluding Fritschea and Hamiltonella ) from 180 whitely individuals collected from six host plants belonging to families Solanaceae (Brinjal, Tomato and Potato) and Fabaceae (Soyabean, Mungbean and Subabool)...
2018: Communicative & Integrative Biology
Sándor Hornok, Krisztina Szőke, Péter Estók, Aleksandra Krawczyk, Anne-Jifke Haarsma, Dávid Kováts, Sándor A Boldogh, Pál Morandini, Sándor Szekeres, Nóra Takács, Jenő Kontschán, Marina L Meli, Isabel G Fernández de Mera, José de la Fuente, Miklós Gyuranecz, Kinga M Sulyok, Beatrice Weibel, Enikő Gönczi, Arnout de Bruin, Hein Sprong, Regina Hofmann-Lehmann
In Europe, several species of bats, owls and kestrels exemplify highly urbanised, flying vertebrates, which may get close to humans or domestic animals. Bat droppings and bird pellets may have epidemiological, as well as diagnostic significance from the point of view of pathogens. In this work 221 bat faecal and 118 bird pellet samples were screened for a broad range of vector-borne bacteria using PCR-based methods. Rickettsia DNA was detected in 13 bat faecal DNA extracts, including the sequence of a rickettsial insect endosymbiont, a novel Rickettsia genotype and Rickettsia helvetica...
February 28, 2018: Antonie Van Leeuwenhoek
Gaowa, Wulantuya, Xuhong Yin, Minzhi Cao, Shengchun Guo, Chunlian Ding, Lu Yuhua, Luo Jianchang, Hiroki Kawabata, Shuji Ando, Hongru Su, Masahiko Shimada, Naoya Takamoto, Yuko Shimamura, Shuichi Masuda, Norio Ohashi
Anaplasma phagocytophilum is an obligate intracellular bacterium and causes febrile illness in humans and livestock. A 49-year-old woman was suffering from feverish symptom, fatigue, arthralgia, general body pain, and anorexia for 2 weeks. Later, she visited Bayan Nur Centers for Disease Control and Prevention Hospital in Inner Mongolia, China. Molecular-based diagnostic analysis of the patient's blood revealed that A. phagocytophilum p44 DNA was positive, but Brucella omp31, spotted fever group Rickettsia gltA, Orientia tsutsugamushi 16S rDNA, and Ehrlichia p28 were negative...
February 28, 2018: Japanese Journal of Infectious Diseases
Gail M Moraru, José Santos Portugal, Andrea Bednarova, Sarah J Mcinnis, Christopher D Paddock, Tom Becker, Travis C Smith, Zdenka Svobodova, Jerome Goddard
Dermacentor parumapertus Neumann (Acari: Ixodidae), a tick primarily associated with rabbits which occurs over much of the western United States, has a fairly large north-to-south distribution, being found from central Idaho southward into northern Mexico. This mostly obscure tick species has recently been the focus of attention due to the discovery of a unique strain of Rickettsia parkeri associated with it which appears closely related to a Rickettsia sp. found in the Atlantic rainforest of Brazil. Historically, a morphological variety of this species was reported in the literature based on significant variation in ornamentation of the tick throughout its range...
February 27, 2018: Journal of Medical Entomology
Álvaro A Faccini-Martínez, María L Félix, María T Armua-Fernandez, José M Venzal
Rickettsia parkeri, a member of the spotted fever group (SFG) rickettsiae, was first confirmed as an etiological agent of human rickettsiosis in 2004. Nearly all cases are characterized by an inoculation eschar, and no fatalities have been reported. In Uruguay, probable human cases of R. parkeri infection (confused initially with R. conorii infection) have been described since 1990 using the clinical name "cutaneous-ganglionar" rickettsiosis. This is the only tick-borne rickettsiosis reported in the country...
February 21, 2018: Ticks and Tick-borne Diseases
Valentina Chisu, Cipriano Foxi, Roberto Mannu, Giuseppe Satta, Giovanna Masala
Sardinia is a hotspot for studying tick-borne diseases in the Mediterranean region, where cases of notifiable tick-borne diseases are increasing. The aim of this study was to determine the presence of tick-borne bacteria of medical and veterinary importance in ixodid ticks collected from domestic and wild animals, humans, and vegetation from different collection sites in Sardinia. Using standard PCR and sequencing techniques, the presence of Rickettsia, Anaplasma, Ehrlichia, and Bartonella species, as well as Coxiella burnetii was evaluated...
February 17, 2018: Ticks and Tick-borne Diseases
Pramod Kumar Maurya, Swati Singh, Ashutosh Mani
Antibiotic resistance is increasing rapidly in pathogenic organisms, creating more complications for treatment of diseases. Rocky Mountain spotted fever (RMSF) is a neglected tropical disease in humans caused by Rickettsia rickettsii for which no effective therapeutic is available. Subtractive genomics methods facilitate the characterization of non-homologous essential proteins that could be targeted for the discovery of potential therapeutic compounds against R. rickettsii to combat RMSF. Present study followed an in-silico based methodology, involving scanning and filtering the complete proteome of Rickettsia rickettsii by using several prioritization parameters in the search of potential candidates for drug development...
February 20, 2018: Acta Tropica
Ashley Howard, Jaime Fergie
BACKGROUND: Murine typhus is a zoonotic infection caused by Rickettsia typhi that remains endemic in South Texas. In 2003, only 9 Texas counties reported murine typhus compared with 41 counties in 2013. METHODS: A retrospective study of children discharged with a confirmed diagnosis of murine typhus from Driscoll Children's Hospital between January 1998-September 2016. RESULTS: 213 children (113 female) 3 months through 19 years of age (mean, 11...
February 19, 2018: Pediatric Infectious Disease Journal
Paul Torpiano, David Pace
BACKGROUND: Mediterranean Spotted Fever (MSF) is a tick-borne zoonosis caused by Rickettsia conorii which is endemic in Malta, an island in the South Mediterranean that is a popular tourist destination. Diagnosis is frequently based on clinical manifestations as laboratory results are often limited to a retrospective diagnosis. We describe the clinical presentation, diagnosis and treatment of children <16 years who presented with MSF from 2011 to 2016. METHOD: The demographics, clinical findings, laboratory results, management and outcome of all children hospitalised with suspected MSF based on the presence of fever and an eschar, were retrieved from their case notes...
February 17, 2018: Travel Medicine and Infectious Disease
Dzul-Rosado Karla, Balam-Romero Javier, Valencia-Pacheco Guillermo, Lugo-Caballero Cesar, Arias-Leon Juan Jose, Peniche-Lara Gaspar, Zavala-Castro Jorge
Background & objectives: The nature of the rickettsial antigens and the immune response generated by them, have been the subject of exhaustive research so that a suitable vaccine can be developed. Till date evaluations of Rickettsia rickettsii antigens that induce both humoral and cellular responses in animal models have only shown partial protection and short-term immunological memory. This study was aimed to evaluate the immune response induced by DNA plasmids generated from the OmpA and OmpB genes of R...
October 2017: Journal of Vector Borne Diseases
Rafal Tokarz, Nischay Mishra, Teresa Tagliafierro, Stephen Sameroff, Adrian Caciula, Lokendrasingh Chauhan, Jigar Patel, Eric Sullivan, Azad Gucwa, Brian Fallon, Marc Golightly, Claudia Molins, Martin Schriefer, Adriana Marques, Thomas Briese, W Ian Lipkin
Tick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses to 8 major tick-borne pathogens present in the United States, including Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, Ehrlichia chaffeensis, Rickettsia rickettsii, Heartland virus and Powassan virus...
February 16, 2018: Scientific Reports
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