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https://www.readbyqxmd.com/read/28448211/prevalence-and-geographic-distribution-of-vector-borne-pathogens-in-apparently-healthy-dogs-in-croatia
#1
Vladimir Mrljak, Josipa Kuleš, Željko Mihaljević, Marin Torti, Jelena Gotić, Martina Crnogaj, Tatjana Živičnjak, Iva Mayer, Iva Šmit, Mangesh Bhide, Renata Barić Rafaj
Vector-borne pathogens (VBPs) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. The aim of the present study was to investigate the seroprevalence against Babesia canis, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Leishmania infantum, Dirofilaria immitis, and Ehrlichia canis in dogs in Croatia. We investigated 435 randomly selected apparently healthy dogs in 13 different locations of Croatia for antibodies to B. canis by indirect immunofluorescence using a commercial IFA IgG Antibody Kit...
April 27, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28438220/anaplasma-spp-in-dogs-and-owners-in-north-western-morocco
#2
Sarah Elhamiani Khatat, Sylvie Daminet, Malika Kachani, Christian M Leutenegger, Luc Duchateau, Hamid El Amri, Mony Hing, Rahma Azrib, Hamid Sahibi
BACKGROUND: Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. However, only one recent article reports on the human esposure to A. phagocytophilum in Morocco and no data are available on canine exposure to both bacteria. Therefore, we conducted a cross-sectional epidemiological study aiming to assess both canine and human exposure to Anaplasma spp...
April 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28438088/anaplasma-ehrlichia-and-rickettsia-species-infections-in-cats
#3
Maria Grazia Pennisi, Regina Hofmann-Lehmann, Alan D Radford, Séverine Tasker, Sándor Belák, Diane D Addie, Corine Boucraut-Baralon, Herman Egberink, Tadeusz Frymus, Tim Gruffydd-Jones, Katrin Hartmann, Marian C Horzinek, Margaret J Hosie, Albert Lloret, Hans Lutz, Fulvio Marsilio, Etienne Thiry, Uwe Truyen, Karin Möstl
Anaplasma species, Ehrlichia species and Rickettsia species are vector-borne pathogens infecting a wide variety of mammals, but causing disease in very few of them. Infection in cats: Anaplasma phagocytophilum is the most important feline pathogen among these rickettsial organisms, and coinfections are possible. Little information is available on the pathogenesis of these agents in cats. Clinical signs are usually reported soon after tick infestation. They are mostly non-specific, consisting of fever, anorexia and lethargy...
May 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28435891/detection-of-anaplasma-phagocytophilum-babesia-microti-borrelia-burgdorferi-borrelia-miyamotoi-and-powassan-virus-in-ticks-by-a-multiplex-real-time-reverse-transcription-pcr-assay
#4
Rafal Tokarz, Teresa Tagliafierro, D Moses Cucura, Ilia Rochlin, Stephen Sameroff, W Ian Lipkin
Ixodes scapularis ticks are implicated in transmission of Anaplasma phagocytophilum, Borrelia burgdorferi, Borrelia miyamotoi, Babesia microti, and Powassan virus. We describe the establishment and implementation of the first multiplex real-time PCR assay with the capability to simultaneously detect and differentiate all five pathogens in a single reaction. The application of this assay for analysis of ticks at sites in New York and Connecticut revealed a high prevalence of B. microti in ticks from Suffolk County, NY...
March 2017: MSphere
https://www.readbyqxmd.com/read/28423085/molecular-survey-of-anaplasma-platys-and-ehrlichia-canis-in-dogs-from-campo-grande-mato-grosso-do-sul-brazil
#5
Rodrigo Soares, Carlos Alberto Ramos, Thatianna Pedroso, Verônica Babo-Terra, Herbert Cleveland, Flábio DE Araújo
This study investigated the frequency of infection by Anaplasma platys and Ehrlichia canis in dogs submitted to animal health centers in Campo Grande, state of Mato Grosso do Sul, Brazil. E. canis and A. platys showed infection frequencies of 55.75% and 16.96%, respectively. The identity of the two species was confirmed by DNA sequencing.
January 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28410950/detection-of-anaplasma-spp-and-ehrlichia-spp-anibodies-and-dirofilaria-immitis-antigens-in-dogs-from-seven-locations-of-morocco
#6
Sarah Elhamiani Khatat, Khalid Khallaayoune, Nabil Errafyk, Frans Van Gool, Luc Duchateau, Sylvie Daminet, Malika Kachani, Hamid El Amri, Rahma Azrib, Hamid Sahibi
In Morocco no data has been published on canine exposure to Anaplasma spp., Borrrelia burgdorferi, and Ehrlichia spp., and only one report is available on the occurrence of Dirofilaria immitis in dogs. Therefore, the aim of this study was to collect current data on the canine exposure to these vector-borne pathogens (VBPs) in Morocco. A total of 217 urban (n=57), rural (n=110) and military (n=50) dogs from seven Moroccan locations were screened for Anaplasma spp., B. burgdorferi and Ehrlichia spp. antibodies and for D...
April 4, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28410148/remodeling-of-tick-cytoskeleton-in-response-to-infection-with-anaplasma-phagocytophilum
#7
Alejandro Cabezas-Cruz, Pilar Alberdi, James J Valdes, Margarita Villar, Jose de la Fuente
The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I...
June 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28408334/the-transcription-factor-relish-controls-anaplasma-marginale-infection-in-the-bovine-tick-rhipicephalus-microplus
#8
J Capelli-Peixoto, Danielle D Carvalho, Wendell C Johnson, Glen A Scoles, Andrea C Fogaça, Sirlei Daffre, Massaro W Ueti
Rhipicephalus microplus is an important biological vector of Anaplasma marginale, the etiological agent of bovine anaplasmosis. The knowledge of tick immune responses to control bacterial infections remains limited. In this study, we demonstrate that transcription factor Relish from the IMD signaling pathway has an important role in the control of A. marginale infection in ticks. We found that RNA-mediated silencing of Relish caused a significant increase in the number of A. marginale in the midgut and salivary glands of R...
April 10, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28381313/influence-of-genetic-background-on-hematologic-and-histopathologic-alterations-during-acute-granulocytic-anaplasmosis-in-129-svev-and-c57bl-6j-mice-lacking-type-i-and-type-ii-interferon-signaling
#9
Jennifer L Johns, Marielle L Discipulo, Amanda L Koehne, Kaitlin A Moorhead, Claude M Nagamine
The role of host type I IFN signaling and its interaction with other immune pathways during bacterial infections is incompletely understood. Type II IFN signaling plays a key role during numerous bacterial infections including granulocytic anaplasmosis (GA) caused by Anaplasma phagocytophilum infection. The function of combined type I and type II IFN signaling and their potential synergism during GA and similar tick-borne diseases is a topic of current research investigation. The goal of this study was to evaluate 2 mouse models of absent type I/type II IFN signaling in experimental A...
March 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28378988/serological-evidence-of-exposure-to-zoonotic-tick-borne-bacteria-in-pheasants-phasianus-colchicus
#10
Valentina Virginia Ebani, Fabrizio Bertelloni, Paolo Mani
INTRODUCTION AND OBJECTIVE: Previous studies on tick-borne pathogens in the avian population have focused mainly on the detection of the agents in ticks collected from birds, but data about the presence of tick-borne bacteria in these animals are scant. The aim of the presented study was to verify the exposure to some zoonotic tick-borne bacteria, in particular, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Coxiella burnetii, and the Rickettsia species of the Spotted Fever Group (SFG), in pheasants (Phasianus colchicus) living in a central Italy area, characterized by conditions favourable for the diffusion of the ticks...
March 22, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28378977/prevalence-of-infections-and-co-infections-with-6-pathogens-in-dermacentor-reticulatus-ticks-collected-in-eastern-poland
#11
Violetta Zając, Angelina Wójcik-Fatla, Anna Sawczyn, Ewa Cisak, Jacek Sroka, Anna Kloc, Zbigniew Zając, Alicja Buczek, Jacek Dutkiewicz, Katarzyna Bartosik
Occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. Accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to Ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. Taking into consideration the role of Dermacentor reticulatus in the transmission of various pathogens, we carried out for the first time the comprehensive statistical analysis of co-infections occurring in this tick species...
March 21, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28358861/pcr-diagnosis-of-tick-borne-pathogens-in-maharashtra-state-india-indicates-fitness-cost-associated-with-carrier-infections-is-greater-for-crossbreed-than-native-cattle-breeds
#12
Sunil W Kolte, Stephen D Larcombe, Suresh G Jadhao, Swapnil P Magar, Ganesh Warthi, Nitin V Kurkure, Elizabeth J Glass, Brian R Shiels
Tick-borne pathogens (TBP) are responsible for significant economic losses to cattle production, globally. This is particularly true in countries like India where TBP constrain rearing of high yielding Bos taurus, as they show susceptibility to acute tick borne disease (TBD), most notably tropical theileriosis caused by Theileria annulata. This has led to a programme of cross breeding Bos taurus (Holstein-Friesian or Jersey) with native Bos indicus (numerous) breeds to generate cattle that are more resistant to disease...
2017: PloS One
https://www.readbyqxmd.com/read/28351721/a-rapid-simple-and-sensitive-loop-mediated-isothermal-amplification-method-to-detect-anaplasma-bovis-in-sheep-and-goats-samples
#13
Jinhong Wang, Yan Zhang, Yanyan Cui, Yaqun Yan, Xiaoxing Wang, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning
A loop-mediated isothermal amplification (LAMP) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. In this study, a set of four LAMP primers was designed to specifically test Anaplasma bovis. The LAMP assay was performed at 62°C for 60min in a water bath. The specificity was confirmed by amplifying A. bovis isolate, while no cross reaction was observed with other five pathogens (Anaplasma bovis, Anaplasma phagocytophilum, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum)...
March 25, 2017: Parasitology International
https://www.readbyqxmd.com/read/28335825/canine-tick-borne-diseases-in-pet-dogs-from-romania
#14
Martin O Andersson, Conny Tolf, Paula Tamba, Mircea Stefanache, Jonas Waldenström, Gerhard Dobler, Lidia Chițimia-Dobler
BACKGROUND: Tick-borne diseases are of substantial concern worldwide for animals as well as humans. Dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. Tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. In addition, co-infections with two or more pathogens enhance this problem further. Molecular methods are useful to disentangle co-infections and to accurately describe prevalence and geographical distribution of tick-borne diseases...
March 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28335329/nanoparticle-based-delivery-of-anaplasma-marginale-membrane-proteins-virb9-1-and-virb10-produced-in-the-pichia-pastoris-expression-system
#15
Bing Zhang, Antonio S Cavallaro, Karishma T Mody, Jun Zhang, James R Deringer, Wendy C Brown, Timothy J Mahony, Chengzhong Yu, Neena Mitter
Bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite Anaplasma marginale in tropical and subtropical areas of the world. While difficult to express, the proteins VirB9-1 and VirB10 are immunogenic components of the outer membrane type IV secretion system that have been identified as candidate antigens for vaccines targeting of A. marginale. Soluble VirB9-1 and VirB10 were successfully expressed using Pichia pastoris. When formulated with the self-adjuvanting silica vesicles, SV-100 (diameter: 50 nm, and pore entrance size: 6 nm), 200 µg of VirB9-1 and VirB10 were adsorbed per milligram of nanoparticle...
November 5, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28331130/cytopenias-and-clonal-expansion-of-gamma-delta-t-cells-in-a-patient-with-anaplasmosis-a-potential-diagnostic-pitfall
#16
Daniel Marko, Anamarija M Perry, Arjuna Ponnampalam, Michel R Nasr
Human granulocytic anaplasmosis is a rare, tick-borne infectious disease caused by Anaplasma phagocytophilum. Herein, we report a rare case of human granulocytic anaplasmosis associated with cytopenias and clonal expansion of gamma/delta T-cells in the bone marrow. A 77-year old man presented multiple times to the emergency department complaining of muscle weakness. Complete blood count detected cytopenias and peripheral blood smear showed pseudo Pelger-Huet neutrophils. These findings prompted bone marrow evaluation with ancillary studies including flow cytometry, karyotyping and T-cell rearrangement studies...
2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28322187/influence-of-genetic-background-on-hematologic-and-histopathologic-alterations-during-acute-granulocytic-anaplasmosis-in-129-svev-and-c57bl-6j-mice-lacking-type-i-and-type-ii-interferon-signaling
#17
Jennifer L Johns Marielle L Discipulo Amanda L Koehne Kaitlin A Moorhead And Claude M Nagamine
The role of host type I IFN signaling and its interaction with other immune pathways during bacterial infections is incompletelyunderstood. Type II IFN signaling plays a key role during numerous bacterial infections including granulocytic anaplasmosis (GA)caused by Anaplasma phagocytophilum infection. The function of combined type I and type II IFN signaling and their potentialsynergism during GA and similar tick-borne diseases is a topic of current research investigation. The goal of this study was toevaluate 2 mouse models of absent type I/type II IFN signaling in experimental A...
March 20, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28298245/serological-and-molecular-prevalence-of-canine-vector-borne-diseases-cvbds-in-korea
#18
Guk-Hyun Suh, Kyu-Sung Ahn, Jong-Ho Ahn, Ha-Jung Kim, Christian Leutenegger, SungShik Shin
BACKGROUND: Previous surveys in dogs from Korea indicated that dogs are exposed to a variety of vector- borne pathogens, but perception for a nation-wide canine vector-borne disease (CVBD) occurrence has been missing. We report here results of both serological and molecular prevalence studies for major CVBDs of dogs from all over the South Korean Peninsula except for Jeju Island. RESULTS: Serological survey of 532 outdoor dogs revealed the highest prevalence for Dirofilaria immitis (25...
March 16, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28288773/serological-detection-of-antibodies-to-anaplasma-spp-borrelia-burgdorferi-sensu-lato-and-ehrlichia-canis-and-of-dirofilaria-immitis-antigen-in-dogs-from-costa-rica
#19
Víctor M Montenegro, Marta C Bonilla, Darwin Kaminsky, Juan José Romero-Zúñiga, Susanne Siebert, Friederike Krämer
In a study in Costa Rica 314 serum samples from dogs throughout all seven provinces were tested using a commercial kit for the detection of circulating antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato and Ehrlichia canis, and of circulating antigen of Dirofilaria immitis. A total of 6.4% (20/314) and 38.2% (120/314) were positive for Anaplasma spp. (An) and E. canis (Ec) antibodies. Overall, 8.0% (25/314) were positive for D. immitis (Di) antigen. One single dog reacted positive with B. burgdorferi s...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28285601/canine-leishmaniosis-caused-by-leishmania-major-and-leishmania-tropica-comparative-findings-and-serology
#20
Gad Baneth, Daniel Yasur-Landau, Matan Gilad, Yaarit Nachum-Biala
BACKGROUND: Infection and clinical disease associated with Leishmania major and Leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. This study describes dogs infected with these Leishmania spp. prevalent in the Middle East and North Africa, and compares the serological response of dogs infected with Leishmania infantum, L. major or L. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (ELISA) of each species and to rK39 dipstick...
March 13, 2017: Parasites & Vectors
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