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Anaplasma

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https://www.readbyqxmd.com/read/29160357/co-infection-with-arthropod-borne-pathogens-in-domestic-cats
#1
Marcos Rogério André, Kilder Dantas Filgueira, Ana Cláudia Calchi, Keyla Carstens Marques de Sousa, Luiz Ricardo Gonçalves, Vitor Brasil Medeiros, Poliana Araújo Ximenes, Ivana Cristina Nunes Gadelha Lelis, Maria Vanuza Nunes de Meireles, Rosangela Zacarias Machado
The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, 'Candidatus Mycoplasma haemominutum', Ehrlichia sp...
November 9, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/29155863/molecular-detection-of-tick-borne-pathogens-in-cattle-from-southwestern-ethiopia
#2
Zerihun Hailemariam, Jürgen Krücken, Maximilian Baumann, Jabbar S Ahmed, Peter-Henning Clausen, Ard M Nijhof
Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of resource-poor farming communities worldwide. In Ethiopia, detailed studies on the epidemiology of tick-borne pathogens (TBPs) in cattle using sensitive molecular detection methods are scarce. The objective of this study was to determine the prevalence and species composition of bovine TBPs of veterinary significance in local cattle populations. A comprehensive cross-sectional epidemiological study was conducted in cattle populations of Illubabor zone in Southwestern Ethiopia from June to August 2013...
2017: PloS One
https://www.readbyqxmd.com/read/29155280/molecular-analysis-of-tick-borne-protozoan-and-rickettsial-pathogens-in-small-ruminants-from-two-south-african-provinces
#3
Aaron Edmond Ringo, Paul Franck Adjou Moumouni, Moeti Taioe, Charoonluk Jirapattharasate, Mingming Liu, Guanbo Wang, Yang Gao, Huanping Guo, Seung-Hun Lee, Weiqing Zheng, Artemis Efstratiou, Jixu Li, Noboru Inoue, Hiroshi Suzuki, Oriel Thekisoe, Xuenan Xuan
Tick-borne protozoan and rickettsial diseases are a major threat to livestock in tropical and sub-tropical regions of Africa. In this study we investigated the presence and distribution of Theileria spp., Babesia ovis, Anaplasma ovis, Anaplasma phagocytophilum, Ehrlichia ruminantium and SFG Rickettsia in sheep and goats from Free State and KwaZulu-Natal provinces. A total of 91 blood samples were screened in this study, 61 from goats and 30 from sheep. PCR assay was conducted using primers based on Theileria spp...
November 15, 2017: Parasitology International
https://www.readbyqxmd.com/read/29141715/clinical-isolation-of-anaplasma-phagocytophilum-in-south-korea
#4
Seung Hun Lee, Se Yoon Park, Mi Jin Jang, Ki Ju Choi, Hae Kyung Lee, Young-Uk Cho, Yeong Seon Lee, Sung-Han Kim, Seon Do Hwang
We report the first isolation of Anaplasma phagocytophilum in South Korea. A 61-year-old woman presented with a 6-day history of fever, headache, and myalgia. Initial investigation showed neutropenia and thrombocytopenia. We diagnosed human granulocytic anaplasmosis by microscopic examination and serologic testing. The patient recovered fully without antibiotic therapy. The isolate was obtained from the patient's blood by cell culture and mouse inoculation. Its identity was confirmed by an immunofluorescence assay, sequencing of the 16S rRNA gene, msp2 (p44), and ankA genes, and staining and electron microscopy of morulae of A...
October 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29132409/insight-into-the-genetic-diversity-of-anaplasma-marginale-in-cattle-from-ten-provinces-of-china
#5
Jifei Yang, Rong Han, Zhijie Liu, Qingli Niu, Guiquan Guan, Guangyuan Liu, Jianxun Luo, Hong Yin
BACKGROUND: Anaplasma marginale is an important tick-transmitted rickettsial pathogen of cattle, with worldwide distribution and an important economic impact. The genetic diversity of A. marginale strains has been extensively characterized in different geographical regions throughout the world, while information is limited on studies in China. This study was carried out to determine the prevalence and genetic diversity of A. marginale strains in cattle from ten provinces of China. METHODS: A total of 557 blood samples from cattle were collected and screened for the occurrence of A...
November 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29127991/arthropod-borne-agents-in-wild-orinoco-geese-neochen-jubata-in-brazil
#6
Karin Werther, Mayara de Cássia Luzzi, Luiz Ricardo Gonçalves, Juliana Paula de Oliveira, José Roberto Ferreira Alves Junior, Rosangela Zacarias Machado, Marcos Rogério André
Although Orinoco goose (Neochen jubata) is an anatid species widely distributed in South America, scarce are the reports on the occurrence of arthropod-borne pathogens in this avian species. The present work aimed to verify, by serological and molecular methods, the occurrence of haemosporida piroplasmids and Anaplasmataceae agents in wild Orinoco geese captured in Brazil. Between 2010 and 2014, 62 blood samples were collected from free-living geese captured in the Araguaia River, Goiás State, Brazil. Six geese (10%) were seropositive for Anaplasma phagocytophilum, showing titers ranging from 40 and 80...
December 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/29121976/prevalence-of-tick-borne-pathogens-in-questing-ixodes-ricinus-ticks-in-urban-and-suburban-areas-of-switzerland
#7
Corinne P Oechslin, Daniel Heutschi, Nicole Lenz, Werner Tischhauser, Olivier Péter, Olivier Rais, Christian M Beuret, Stephen L Leib, Sergei Bankoul, Rahel Ackermann-Gäumann
BACKGROUND: Throughout Europe, Ixodes ricinus transmits numerous pathogens. Its widespread distribution is not limited to rural but also includes urbanized areas. To date, comprehensive data on pathogen carrier rates of I. ricinus ticks in urban areas of Switzerland is lacking. RESULTS: Ixodes ricinus ticks sampled at 18 (sub-) urban collection sites throughout Switzerland showed carrier rates of 0% for tick-borne encephalitis virus, 18.0% for Borrelia burgdorferi (sensu lato), 2...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29120859/tick-borne-infections-and-co-infections-in-patients-with-non-specific-symptoms-in-poland-tick-borne-infections-and-co-infections
#8
Justyna Dunaj, Anna Moniuszko-Malinowska, Izabela Swiecicka, Martin Andersson, Piotr Czupryna, Krzysztof Rutkowski, Grzegorz Zambrowski, Joanna Zajkowska, Sambor Grygorczuk, Maciej Kondrusik, Renata Świerzbińska, Sławomir Pancewicz
AIM: The aim of the study was the evaluation of the frequency of infections and co-infections among patients hospitalized because of non-specific symptoms after a tick bite. MATERIALS AND METHODS: Whole blood, serum and cerebrospinal fluid samples from 118 patients hospitalised for non-specific symptoms up to 8 weeks after tick bite from 2010 to 2013 were examined for tick-borne infections. ELISA, Western blot and/or molecular biology (PCR; fla gene; 16S rRNA; sequencing) and thin blood smears (MDD) were used...
November 7, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/29119682/molecular-identification-of-tick-borne-pathogens-infecting-cattle-in-mymensingh-district-of-bangladesh-reveals-emerging-species-of-anaplasma-and-babesia
#9
B C Roy, J Krücken, J S Ahmed, S Majumder, M P Baumann, P-H Clausen, A M Nijhof
Tick-borne diseases are considered a major hindrance to the health and productive performance of cattle in Bangladesh. To elucidate the epidemiology of tick-borne pathogens (TBPs) in local cattle, a cross-sectional study was performed in the 12 subdistricts (Upazilas) of Mymensingh district in Bangladesh. Blood samples and ticks were collected from 384 clinically healthy cattle kept by 135 farmers from 96 randomly selected villages. DNA extracted from the blood samples was subsequently screened by polymerase chain reaction (PCR) and a Reverse Line Blot (RLB) hybridization assay using an in-house prepared chemiluminescence solution for the presence of Anaplasma, Ehrlichia, Rickettsia, Babesia and Theileria spp...
November 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29103397/anaplasmataceae-agents-among-wild-mammals-and-ectoparasites-in-brazil
#10
K C M DE Sousa, A C Calchi, H M Herrera, J S Dumler, D M Barros-Battesti, R Z Machado, M R André
Anaplasmataceae agents comprise obligate intracellular bacteria that can cause disease in humans and animals. Between August 2013 and March 2015, 31 Nasua nasua (coati), 78 Cerdocyon thous (crab-eating fox), seven Leopardus pardalis (ocelot), 110 wild rodents, 30 marsupials, and 42 dogs were sampled in the Pantanal wetland, Brazil. In addition, ectoparasites found parasitizing the animals were collected and identified. The present work aimed to investigate the occurrence of Anaplasmataceae agents in wild mammals, domestic dogs and ectoparasites, by molecular and serological techniques...
November 6, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29089249/groel-typing-and-phylogeny-of-anaplasma-species-in-ticks-from-domestic-and-wild-vertebrates
#11
Valentina Chisu, Rosanna Zobba, Roberta Lecis, Francesca Sotgiu, Giovanna Masala, Cipriano Foxi, Danilo Pisu, Alberto Alberti
Anaplasma species are globally distributed tick-borne bacteria causing a range of clinical conditions in domestic animals, wildlife, and human. Nevertheless, data on presence and distribution of Anaplasma strains in ticks are still lacking, especially in the Mediterranean region. This study reports the molecular identification, genetic characterization and phylogeny of Anaplasma strains of both veterinary and zoonotic importance in ticks collected from domestic and wild hosts sampled in a typical Mediterranean warm temperate region, the island of Sardinia...
October 26, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29056701/prevalence-of-selected-zoonotic-and-vector-borne-agents-in-dogs-and-cats-on-the-pine-ridge-reservation
#12
A Valeria Scorza, Michael R Lappin
The prevalence of intestinal parasites and vector-borne agents of dogs and cats in the Pine Ridge Reservation, South Dakota were determined. Fecal samples (84 dogs, 9 cats) were examined by centrifugal floatation and by immunofluorescence assay (FA) for Giardia and Cryptosporidium. PCR was performed on Giardia [beta-giardin (bg), triose phosphate isomerase (tpi), glutamate dehydrogenase genes (gdh)] and Cryptosporidium [heat shock protein-70 gene (hsp)] FA positive samples. Cat sera (n = 32) were tested for antibodies against Bartonella spp...
September 4, 2017: Veterinary Sciences
https://www.readbyqxmd.com/read/29055641/molecular-detection-and-characterization-of-zoonotic-anaplasma-species-in-domestic-dogs-in-lusaka-zambia
#13
Pipina A Vlahakis, Simbarashe Chitanga, Martin C Simuunza, Edgar Simulundu, Yongjin Qiu, Katendi Changula, Herman M Chambaro, Masahiro Kajihara, Ryo Nakao, Ayato Takada, Aaron S Mweene
Although tick-borne pathogens, Anaplasma platys and Anaplasma phagocytophilum are recognized as zoonotic agents associated with appreciable morbidity and mortality in dogs and humans worldwide, there is limited information on these infections in many African countries, including Zambia. The purpose of this study was to detect, identify and phylogenetically characterize Anaplasma species from dogs in Chilanga District in Lusaka Province, Zambia. A total of 301 blood samples were collected from apparently healthy and semi-confined dogs...
October 17, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29054570/estimated-seroprevalence-of-anaplasma-spp-and-spotted-fever-group-rickettsia-exposure-among-herders-and-livestock-in-mongolia
#14
Michael E von Fricken, Sukhbaatar Lkhagvatseren, Bazartseren Boldbaatar, Pagbajab Nymadawa, Thomas A Weppelmann, Bekh-Ochir Baigalmaa, Benjamin D Anderson, Megan E Reller, Paul M Lantos, Gregory C Gray
BACKGROUND: To better understand the epidemiology of tick-borne disease in Mongolia, a comprehensive seroprevalence study was conducted investigating exposure to Anaplasma spp. and spotted fever group (SFG) Rickettsia spp. in nomadic herders and their livestock across three provinces from 2014 to 2015. METHODS: Blood was collected from 397 herders and 2370 livestock, including sheep, goats, cattle, horses and camels. Antibodies against Anaplasma spp. and SFG Rickettsia were determined by indirect immunofluorescence using commercially available slides coated with Anaplasma phagocytophilum and Rickettsia rickettsii antigens...
January 2018: Acta Tropica
https://www.readbyqxmd.com/read/29047399/pathogen-communities-of-songbird-derived-ticks-in-europe-s-low-countries
#15
Dieter Heylen, Manoj Fonville, Arieke Docters van Leeuwen, Arjan Stroo, Martin Duisterwinkel, Sip van Wieren, Maria Diuk-Wasser, Arnout de Bruin, Hein Sprong
BACKGROUND: Birds play a major role in the maintenance of enzootic cycles of pathogens transmitted by ticks. Due to their mobility, they affect the spatial distribution and abundance of both ticks and pathogens. In the present study, we aim to identify members of a pathogen community [Borrelia burgdorferi (s.l.), B. miyamotoi, 'Ca. Neoehrlichia mikurensis', Anaplasma phagocytophilum and Rickettsia helvetica] in songbird-derived ticks from 11 locations in the Netherlands and Belgium (2012-2014)...
October 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29038575/human-rickettsial-pathogen-modulates-arthropod-organic-anion-transporting-polypeptide-and-tryptophan-pathway-for-its-survival-in-ticks
#16
Vikas Taank, Shovan Dutta, Amrita Dasgupta, Tanner K Steeves, Durland Fish, John F Anderson, Hameeda Sultana, Girish Neelakanta
The black-legged tick Ixodes scapularis transmits the human anaplasmosis agent, Anaplasma phagocytophilum. In this study, we show that A. phagocytophilum specifically up-regulates I. scapularis organic anion transporting polypeptide, isoatp4056 and kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), for its survival in ticks. RNAi analysis revealed that knockdown of isoatp4056 expression had no effect on A. phagocytophilum acquisition from the murine host but affected the bacterial survival in tick cells...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29037826/occurrence-of-four-anaplasma-species-with-veterinary-and-public-health-significance-in-sheep-northwestern-china
#17
Jifei Yang, Rong Han, Qingli Niu, Zhijie Liu, Guiquan Guan, Guangyuan Liu, Jianxun Luo, Hong Yin
The members of the genus Anaplasma are important tick-borne rickettsial bacteria of veterinary and public health significance. Domestic ruminants are important reservoir hosts for several Anaplasma species. In this study, the occurrence of Anaplasma spp. was investigated by PCR in domestic small ruminants from Gansu, northwestern China. The results showed a high prevalence of Anaplasma spp. (46.2%, 201/435) in sheep. The average infection rates were 5.7%, 24.4%, 28.0% and 18.2% for A. ovis, A. bovis, A. phagocytophilum and A...
October 12, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29034616/molecular-survey-and-genetic-characterization-of-anaplasma-centrale-a-marginale-and-a-bovis-in-cattle-from-algeria
#18
M R Rjeibi, O Ayadi, M Rekik, M Gharbi
Bovine anaplasmosis could be caused by several Anaplasma species. The causative agents are transmitted by ticks and haematophagous arthropods with a high impact on both human and animal health. This study was conducted to estimate the infection rate and to characterize Anaplasma spp. in cattle from Algeria. A molecular survey was performed in Setif district (Northeast Algeria) where a total number of 180 cattle blood samples were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes...
October 16, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29029144/broad-range-survey-of-vector-borne-pathogens-and-tick-host-identification-of-ixodes-ricinus-from-southern-czech-republic
#19
Vaclav Honig, Heather E Carolan, Zuzana Vavruskova, Christian Massire, Michael R Mosel, Christopher D Crowder, Megan A Rounds, David J Ecker, Daniel Ruzek, Libor Grubhoffer, Benjamin J Luft, Mark W Eshoo
Ixodes ricinus ticks are vectors of numerous human and animal pathogens. They are host generalists able to feed on more than 300 vertebrate species. The prevalence of tick-borne pathogens is influenced by host-vector-pathogen interactions that results in spatial distribution of infection risk. Broad-range polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was used to analyze 435 I. ricinus nymphs from four localities in the south of the Czech Republic for the species identification of tick-borne pathogens...
November 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29022768/molecular-survey-on-rickettsia-spp-anaplasma-phagocytophilum-borrelia-burgdorferi-sensu-lato-and-babesia-spp-in-ixodes-ricinus-ticks-infesting-dogs-in-central-italy
#20
Giulia Morganti, Stefano Gavaudan, Cristina Canonico, Silvia Ravagnan, Emanuela Olivieri, Manuela Diaferia, Maria Luisa Marenzoni, Maria Teresa Antognoni, Gioia Capelli, Cornelia Silaghi, Fabrizia Veronesi
Dogs are a common feeding hosts for Ixodes ricinus and may act as reservoir hosts for zoonotic tick-borne pathogens (TBPs) and as carriers of infected ticks into human settings. The aim of this work was to evaluate the presence of several selected TBPs of significant public health concern by molecular methods in I. ricinus recovered from dogs living in urban and suburban settings in central Italy. A total of 212 I. ricinus specimens were collected from the coat of domestic dogs. DNA was extracted from each specimen individually and tested for Rickettsia spp...
November 2017: Vector Borne and Zoonotic Diseases
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