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https://www.readbyqxmd.com/read/28939494/prevalence-risk-factors-and-vectors-identification-of-bovine-anaplasmosis-and-babesiosis-in-and-around-jimma-town-southwestern-ethiopia
#1
Nejash Abdela, Nuraddis Ibrahim, Feyissa Begna
Among tick-borne diseases, bovine anaplasmosis and babesiosis are considered to be one of the most important in ruminants worldwide, causing significant economic losses in tropical and subtropical areas. This cross-sectional study was therefore undertaken from November 2016 to April 2017 with the objectives to assess the prevalence and potential risk factors associated with bovine anaplasmosis and babesiosis and also to identify the vectors involved in transmission of these diseases in and around Jimma town, south western Ethiopia...
September 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28931077/detection-of-bacterial-pathogens-including-potential-new-species-in-human-head-lice-from-mali
#2
Nadia Amanzougaghene, Florence Fenollar, Abdoul Karim Sangaré, Mahamadou S Sissoko, Ogobara K Doumbo, Didier Raoult, Oleg Mediannikov
In poor African countries, where no medical and biological facilities are available, the identification of potential emerging pathogens of concern at an early stage is challenging. Head lice, Pediculus humanus capitis, have a short life, feed only on human blood and do not transmit pathogens to their progeny. They are, therefore, a perfect tool for the xenodiagnosis of current or recent human infection. This study assessed the occurrence of bacterial pathogens from head lice collected in two rural villages from Mali, where a high frequency of head lice infestation had previously been reported, using molecular methods...
2017: PloS One
https://www.readbyqxmd.com/read/28928415/cultivation-of-anaplasma-ovis-in-the-hl-60-human-promyelocytic-leukemia-cell-line
#3
Ran Wei, Hong-Bo Liu, Frans Jongejan, Bao-Gui Jiang, Qiao-Cheng Chang, Xue Fu, Jia-Fu Jiang, Na Jia, Wu-Chun Cao
The tick-borne bacterium Anaplasma ovis is a widely distributed pathogen affecting sheep, goats and wild ruminants. Here, the HL-60 human promyelocytic leukemia cell line was used to isolate A. ovis from PCR-positive sheep and goats in Heilongjiang Province, China. Two weeks after inoculation, morulae were observed in cytoplasmic vacuoles in four different HL-60 cultures. Confocal microscopy using a Cy3-labeled A. ovis-specific probe confirmed that the HL-60 cells were infected with A. ovis. Cells from the 6th HL-60 subculture displayed positive fluorescence when incubated with A...
September 20, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28915997/outbreak-of-anaplasmosis-associated-with-novel-genetic-variants-of-anaplasma-marginale-in-a-dairy-cattle
#4
Munir Aktas, Sezayi Özübek
During early lactation, dairy cows may present a transient immunosuppressive state and develop anaplasmosis caused by Anaplasma marginale. In this study, clinical anaplasmosis in dairy cattle in the Thrace region of Turkey was investigated with respect to within-herd prevalence, vertical transmission, and genetic diversity. In March and September 2015, thirty lactating cows showed primary clinical signs of anaplasmosis, including fever, anaemia, decreased milk yield, anorexia, and laboured breathing. Symptoms disappeared in most cows after administration of long-acting oxytetracycline, but nine of them (30%) died...
October 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28911844/serologic-responses-to-peptides-of-anaplasma-phagocytophilum-and-borrelia-burgdorferi-in-dogs-infested-with-wild-caught-ixodes-scapularis
#5
R Chandrashekar, M J Beall, B Thatcher, J M Saucier, P Tyrrell, M R Lappin
Anaplasma phagocytophilum and Borrelia burgdorferi are both transmitted by Ixodes spp. and are associated with clinical illness in some infected dogs. This study evaluated canine antibody responses to the A. phagocytophilum p44 peptides APH-1 and APH-4 as well as the B. burgdorferi C6 peptide before and after doxycycline treatment. A total of eight dogs were infested with wild-caught I. scapularis for 1 week. Blood was collected prior to tick attachment and from Days 3-77 to 218-302 with doxycycline treatment beginning on Day 218...
August 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28905982/vector-borne-diseases-in-cats-in-germany
#6
Michèle Bergmann, Katrin Hartmann
Vector-borne diseases (VBDs) are caused by a wide range of pathogens, which are transmitted by a variety of vectors, such as ticks and fleas. As a result of climate changes, more vector-borne diseases are becoming endemic in Germany, not only in dogs, but also in cats. For some of the pathogens prevalence data still need to be investigated in Germany. However, natural infections with Bartonella, Anaplasma, haemotropic Mycoplasma and Borrelia species have already been described in German cats. Clinical relevance of these pathogens is not fully understood, and it is still unknown, why most infected cats stay asymptomatic and which predisposing factors contribute to the development of clinical signs in cats...
September 13, 2017: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/28905230/molecular-detection-of-anaplasma-platys-anaplasma-phagocytophilum-and-wolbachia-sp-but-not-ehrlichia-canis-in-croatian-dogs
#7
Doroteja Huber, Irena Reil, Sanja Duvnjak, Daria Jurković, Damir Lukačević, Miroslav Pilat, Ana Beck, Željko Mihaljević, Lea Vojta, Adam Polkinghorne, Relja Beck
The bacteria Anaplasma platys, Anaplasma phagocytophilum and Ehrlichia canis are tick-borne agents that cause canine vector-borne disease. The prevalence of these pathogens in South Eastern Europe is unknown with the exception of an isolated case of A. platys detected in a dog imported into Germany from Croatia. To gain a better insight into their presence and prevalence, PCR-based screening for these bacterial pathogens was performed on domesticated dogs from different regions of Croatia. Blood samples from 1080 apparently healthy dogs from coastal and continental parts of Croatia as well as tissue samples collected from 63 deceased dogs with a history of anaemia and thrombocytopenia were collected for molecular screening by an Anaplasmataceae-specific 16S rRNA conventional PCR...
September 14, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28902261/detection-of-anaplasma-sp-phylogenetically-related-to-a-phagocytophilum-in-a-free-living-bird-in-brazil
#8
Anna Claudia Baumel Mongruel, Jyan Lucas Benevenute, Priscila Ikeda, Marcos Rogério André, Rosangela Zacarias Machado, Adriano de Oliveira Torres Carrasco, Meire Christina Seki
Wild animals play an important role in carrying vectors that may potentially transmit pathogens. Several reports highlighted the participation of wild animals on the Anaplasma phagocytophilum cycle, including as hosts of the agent. The aim of this study was to report the molecular detection of an agent phylogenetically related to A. phagocytophilum isolated from a wild bird in the Midwest of the state of Paraná, Brazil. Fifteen blood samples were collected from eleven different bird species in the Guarapuava region...
August 24, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28890111/tick-borne-pathogens-and-their-reservoir-hosts-in-northern-italy
#9
Ivana Baráková, Markéta Derdáková, Diana Selyemová, Michal Chvostáč, Eva Špitalská, Fausta Rosso, Margherita Collini, Roberto Rosà, Valentina Tagliapietra, Matteo Girardi, Claudio Ramponi, Heidi C Hauffe, Annapaola Rizzoli
The aim of this study was to determine the occurrence of Anaplasma phagocytophilum, Rickettsia spp., Babesia spp., and Candidatus Neoehrlichia mikurensis in Ixodes spp. ticks removed from wildlife, domestic animals and humans in the Province of Trento (northern Italy) in order to better understand their ecology and provide public health professionals with an updated list of pathogens which should be considered during their diagnostic procedures after a tick bite. During 2011-2012, 848 feeding ticks at all life stages (adults, nymphs and larvae) from various hosts (wild ungulates, birds and rodents; domestic sheep, dogs and humans) were collected...
September 1, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28888642/spatio-temporal-variations-and-genetic-diversity-of-anaplasma-spp-in-cattle-from-the-north-of-tunisia
#10
Hanène Belkahia, Mourad Ben Said, Narjesse El Mabrouk, Mariem Saidani, Chayma Cherni, Mariem Ben Hassen, Ali Bouattour, Lilia Messadi
In cattle, anaplasmosis is a tick-borne rickettsial disease caused by Anaplasma marginale, A. centrale, A. phagocytophilum, and A. bovis. To date, no information concerning the seasonal dynamics of single and/or mixed infections by different Anaplasma species in bovines are available in Tunisia. In this work, a total of 1035 blood bovine samples were collected in spring (n=367), summer (n=248), autumn (n=244) and winter (n=176) from five different governorates belonging to three bioclimatic zones from the North of Tunisia...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28887102/molecular-detection-of-vector-borne-pathogens-in-greek-cats
#11
Mathios E Mylonakis, Megan Schreeg, Manolis K Chatzis, Julian Pearce, Henry S Marr, Manolis N Saridomichelakis, Adam J Birkenheuer
Infectious diseases have been increasingly recognized in cats worldwide. The objective of this study was the molecular investigation of the prevalence of selected pathogens in healthy and sick cats from Greece, a country highly endemic for several canine vector-borne pathogens. Blood and/or bone marrow samples from 50 clinically healthy and 50 sick adult (>1 year-old) cats were retrospectively examined for the amplification of Bartonella spp., haemoplasmas, Ehrlichia spp., Anaplasma spp., Babesia spp., and Cytauxzoon spp...
September 1, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28882512/epidemiology-of-babesia-anaplasma-and-trypanosoma-species-using-a-new-expanded-reverse-line-blot-hybridization-assay
#12
Martina Soledad Paoletta, Ludmila López Arias, Sofía de la Fournière, Eliana Carolina Guillemi, Carlos Luciani, Néstor Fabián Sarmiento, Juan Mosqueda, Marisa Diana Farber, Silvina Elizabeth Wilkowsky
Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed for the simultaneous detection and identification of Anaplasma, Babesia and bovine trypanosomes, encompassing in this way the most relevant hemoparasites that affect cattle. A total of 186 bovine blood samples collected from two different ecoepidemiological regions of northeast Argentina, with and without tick control, were analyzed with this new RLB...
September 1, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28875270/molecular-evidence-of-tick-borne-pathogens-in-hyalomma-anatolicum-ticks-infesting-cattle-in-xinjiang-uygur-autonomous-region-northwestern-china
#13
Peifa Yu, Zhijie Liu, Qingli Niu, Jifei Yang, Mirza Omar Abdallah, Ze Chen, Guangyuan Liu, Jianxun Luo, Hong Yin
Although tick-borne pathogens have been widely reported in ticks in China, there is little information available on the prevalence of information in Hyalomma ticks from cattle. This study aims to determine the occurrence of pathogens in Hyalomma anatolicum collected from cattle in Xinjiang Uygur Autonomous Region, China, by PCR, sequencing and phylogenetic analysis. Borrelia burgdorferi s.s., Rickettsia massiliae and Anaplasma bovis were identified, whereas DNA of Ehrlichia species and an Anaplasma platys-like pathogen were also detected...
September 5, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28869191/co-infection-with-babesia-divergens-and-anaplasma-phagocytophilum-in-cattle-bos-taurus-sweden
#14
Martin O Andersson, Bronislava Víchová, Conny Tolf, Sandra Krzyzanowska, Jonas Waldenström, Maria E Karlsson
Babesiosis is a severe disease in cattle worldwide. In Europe, the main causative agent of bovine babesiosis is Babesia divergens. In some areas, this species is reported to have declined or even disappeared, and its etiological role overtaken by other piroplasmid species. Moreover, co-infection with other tick-transmitted pathogens can be expected to complicate diagnosis in cattle. Hence, molecular identification of the causative agent of babesiosis should be a priority. Therefore, samples from 71 domestic cattle, 39 with clinical signs of babesiosis and 32 without, from southern Sweden were screened for Babesia spp...
August 24, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28861402/ixodes-scapularis-tick-cells-control-anaplasma-phagocytophilum-infection-by-increasing-the-synthesis-of-phosphoenolpyruvate-from-tyrosine
#15
Alejandro Cabezas-Cruz, Pedro J Espinosa, Dasiel A Obregón, Pilar Alberdi, José de la Fuente
The obligate intracellular pathogen, Anaplasma phagocytophilum, is the causative agent of life-threatening diseases in humans and animals. A. phagocytophilum is an emerging tick-borne pathogen in the United States, Europe, Africa and Asia, with increasing numbers of infected people and animals every year. It is increasingly recognized that intracellular pathogens modify host cell metabolic pathways to increase infection and transmission in both vertebrate and invertebrate hosts. Recent reports have shown that amino acids are central to the host-pathogen metabolic interaction...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28859959/first-confirmed-report-of-outbreak-of-theileriosis-anaplasmosis-in-a-cattle-farm-in-henan-china
#16
Yanyan Cui, Xiaoxing Wang, Yan Zhang, Yaqun Yan, Haiju Dong, Fuchun Jian, Ke Shi, Longxian Zhang, Rongjun Wang, Changshen Ning
Tick-borne diseases (TBDs) impose a significant constraint to livestock production world widely. In this paper, we presented a case of TBD in a cattle farm in Henan, China. 35 blood samples (7 samples sent by veterinarian, 28 samples gathered by our colleagues) were collected from ill, surviving and asymptomatic cattle and microscopic observation and PCR assays were conducted to characterize the pathogens. Genus Ixodes feeding on these cattle were collected and identified. Theileria annulata-like and Anaplasma marginale-like pathogens were observed in the blood smears stained with Giemsa staining under microscope...
August 28, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28856544/molecular-detection-of-pathogens-in-ticks-infesting-cattle-in-nampula-province-mozambique
#17
Ana Marcília Matsimbe, Vlademiro Magaia, Gustavo Seron Sanches, Luís Neves, Emília Noormahomed, Sandra Antunes, Ana Domingos
Ticks are ectoparasites that can act as vectors of a large number of pathogens in wild and domestic animals, pets, and occasionally humans. The global threat of emerging or re-emerging tick-borne diseases supports the need for research focused in the zoonotic transmission, especially in countries like Mozambique where rural populations are in close contact with domestic animals. The present study aims to: (1) identify tick species infesting cattle from Monapo and Nacala Porto, districts of Nampula province, Mozambique; and (2) investigate the presence of pathogens in the collected ticks...
August 30, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28840782/comparison-of-three-blood-transfusion-guidelines-applied-to-31-feline-donors-to-minimise-the-risk-of-transfusion-transmissible-infections
#18
Maria Luisa Marenzoni, Stefania Lauzi, Arianna Miglio, Mauro Coletti, Andrea Arbia, Saverio Paltrinieri, Maria Teresa Antognoni
Objectives The increased demand for animal blood transfusions creates the need for an adequate number of donors. At the same time, a high level of blood safety must be guaranteed and different guidelines (GLs) deal with this topic. The aim of this study was to evaluate the appropriateness of different GLs in preventing transfusion-transmissible infections (TTI) in Italian feline blood donors. Methods Blood samples were collected from 31 cats enrolled as blood donors by the owners' voluntary choice over a period of approximately 1 year...
August 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28835291/occurrence-and-diversity-of-arthropod-transmitted-pathogens-in-red-foxes-vulpes-vulpes-in-western-austria-and-possible-vertical-transplacental-transmission-of-hepatozoon-canis
#19
Adnan Hodžić, Naike Mrowietz, Rita Cézanne, Pia Bruckschwaiger, Sylvia Punz, Verena Elisabeth Habler, Valentina Tomsik, Judit Lazar, Georg G Duscher, Walter Glawischnig, Hans-Peter Fuehrer
Red fox (Vulpes vulpes) is the most abundant wild canid species in Austria, and it is a well-known carrier of many pathogens of medical and veterinary concern. The main aim of the present study was to investigate the occurrence and diversity of protozoan, bacterial and filarial parasites transmitted by blood-feeding arthropods in a red fox population in western Austria. Blood (n = 351) and spleen (n = 506) samples from foxes were examined by PCR and sequencing and the following pathogens were identified: Babesia canis, Babesia cf...
August 24, 2017: Parasitology
https://www.readbyqxmd.com/read/28829732/case-report-polymerase-chain-reaction-testing-of-tick-bite-site-samples-for-the-diagnosis-of-human-granulocytic-anaplasmosis
#20
Choon-Mee Kim, Seok Won Kim, Dong-Min Kim, Na-Ra Yoon, Piyush Jha, Sook Jin Jang, Young-Joon Ahn, Donghoon Lim, Seung Hun Lee, Seon Do Hwang, Yeong Seon Lee
Human granulocytic anaplasmosis (HGA) is a tick-borne infectious disease caused by Anaplasma phagocytophilum, an obligate intracellular bacterium. Until now, the utility of tick-bite site samples for HGA diagnosis has not been reported. Using a patient's buffy coat and tick-bite site crust samples, we performed polymerase chain reaction (PCR) testing using Ehrlichia- or Anaplasma-specific primers. PCR with buffy coat and crust samples obtained before doxycycline administration was positive. Six days after doxycycline administration, PCR with the buffy coat sample was negative but PCR with a crust tissue sample from the tick-bite site remained positive...
August 2017: American Journal of Tropical Medicine and Hygiene
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