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https://www.readbyqxmd.com/read/28214195/first-clinical-case-of-canine-granulocytic-anaplasmosis-in-korea-and-genotypic-analyses-of-anaplasma-phagocytophilum
#1
Seung-Hun Lee, Nara Kim, Dongmi Kwak
A 2-year-old male Jindo dog was presented to a local veterinary clinic for anorexia, lethargy, edema around neck, and vomiting. Based on the clinical history, physical examination, hematology, blood chemistry, serology, and PCR, the dog was diagnosed with canine granulocytic anaplasmosis (CGA). PCR and phylogenetic analyses targeting the 16S rRNA, groEL, and msp2 genes of Anaplasma phagocytophilum revealed that the A. phagocytophilum identified in this study subgrouped into alanine and USA groups according to the groEL and msp2 gene sequences, respectively...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28202075/tick-borne-pathogens-induce-differential-expression-of-genes-promoting-cell-survival-and-host-resistance-in-ixodes-ricinus-cells
#2
Karen L Mansfield, Charlotte Cook, Richard J Ellis, Lesley Bell-Sakyi, Nicholas Johnson, Pilar Alberdi, José de la Fuente, Anthony R Fooks
BACKGROUND: There has been an emergence and expansion of tick-borne diseases in Europe, Asia and North America in recent years, including Lyme disease, tick-borne encephalitis and human anaplasmosis. The primary vectors implicated are hard ticks of the genus Ixodes. Although much is known about the host response to these bacterial and viral pathogens, there is limited knowledge of the cellular responses to infection within the tick vector. The bacterium Anaplasma phagocytophilum is able to bypass apoptotic processes in ticks, enabling infection to proceed...
February 15, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28197924/sumoylation-as-an-integral-mechanism-in-bacterial-infection-and-disease-progression
#3
Chittur V Srikanth, Smriti Verma
Post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. Among these, particularly the modification with Small Ubiquitin-like Modifiers (SUMO) is being recognized as a pathway crucial for cell homeostasis and health. Understandably, bacterial pathogens intervene with the SUMO pathway of the host for ensuring successful infection. Among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. Majority of them including Salmonella Typhimurium, Shigella flexneri and Listeria monocytogenes target the E2 conjugating enzyme Ubc9...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28195158/infection-derived-lipids-elicit-an-immune-deficiency-circuit-in-arthropods
#4
Dana K Shaw, Xiaowei Wang, Lindsey J Brown, Adela S Oliva Chávez, Kathryn E Reif, Alexis A Smith, Alison J Scott, Erin E McClure, Vishant M Boradia, Holly L Hammond, Eric J Sundberg, Greg A Snyder, Lei Liu, Kathleen DePonte, Margarita Villar, Massaro W Ueti, José de la Fuente, Robert K Ernst, Utpal Pal, Erol Fikrig, Joao H F Pedra
The insect immune deficiency (IMD) pathway resembles the tumour necrosis factor receptor network in mammals and senses diaminopimelic-type peptidoglycans present in Gram-negative bacteria. Whether unidentified chemical moieties activate the IMD signalling cascade remains unknown. Here, we show that infection-derived lipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) and 1-palmitoyl-2-oleoyl diacylglycerol (PODAG) stimulate the IMD pathway of ticks. The tick IMD network protects against colonization by three distinct bacteria, that is the Lyme disease spirochete Borrelia burgdorferi and the rickettsial agents Anaplasma phagocytophilum and A...
February 14, 2017: Nature Communications
https://www.readbyqxmd.com/read/28188085/species-of-ticks-and-carried-pathogens-in-owned-dogs-in-spain-results-of-a-one-year-national-survey
#5
Agustín Estrada-Peña, Xavier Roura, Angel Sainz, Guadalupe Miró, Laia Solano-Gallego
This study presents the results of a national survey in Spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. In the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. The primary species of ticks were Rhipicephalus sanguineus s.l. (53%), Dermacentor reticulatus (9%), Ixodes ricinus (9%), and I. hexagonus (4%). Parasitism by two species of these ticks was rare...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28169172/molecular-characterisation-of-anaplasma-species-from-african-buffalo-syncerus-caffer-in-kruger-national-park-south-africa
#6
Danielle Sisson, Jasmin Hufschmid, Anna Jolles, Brianna Beechler, Abdul Jabbar
Bovine anaplasmosis is a tick-borne disease, mainly caused by Anaplasma marginale and A. centrale and is distributed in tropical and sub-tropical areas. This study aimed to characterise A. marginale and A. centrale from African buffaloes in Kruger National Park (KNP), South Africa, using the DNA sequences of the genes coding for major surface protein (msp1β) and heat shock protein (groEL), respectively. A total of 747 blood samples were collected from February 2014 to August 2016 from African buffaloes kept in KNP, and DNAs were tested using a molecular-phylogenetic approach...
January 17, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28160773/-candidatus-anaplasma-camelii-in-one-humped-camels-camelus-dromedarius-in-morocco-a-novel-and-emerging-anaplasma-species
#7
Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-Jean Boulouis, Renaud Maillard
BACKGROUND: There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics...
February 5, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28155283/comparison-of-three-nucleic-acid-based-tests-for-detecting-i-anaplasma-marginale-i-and-i-anaplasma-centrale-i-in-cattle
#8
Mamohale E Chaisi, Janine R Baxter, Paidashe Hove, Chimvwele N Choopa, Marinda C Oosthuizen, Kelly A Brayton, Zamantungwa T H Khumalo, Awelani M Mutshembele, Moses S Mtshali, Nicola E Collins
Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested polymerase chain reaction (nPCR) assays and a duplex real-time quantitative polymerase chain reaction (qPCR) assay to detect A. marginale and A. centrale infections in cattle (n = 66) in South Africa. The lowest detection limits for A...
January 23, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/28153803/development-of-duplex-pcr-for-simultaneous-detection-of-theileria-spp-and-anaplasma-spp-in-sheep-and-goats
#9
Yanyan Cui, Yan Zhang, Fuchun Jian, Longxian Zhang, Rongjun Wang, Shuxuan Cao, Xiaoxing Wang, Yaqun Yan, Changshen Ning
Theileria spp. and Anaplasma spp., which are important tick-borne pathogens (TBPs), impact the health of humans and animals in tropical and subtropical areas. Theileria and Anaplasma co-infections are common in sheep and goats. Following alignment of the relevant DNA sequences, two primer sets were designed to specifically target the Theileria spp. 18S rRNA and Anaplasma spp. 16S rRNA gene sequences. Genomic DNA from the two genera was serially diluted tenfold for testing the sensitivities of detection of the primer sets...
January 31, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28151084/molecular-detection-of-anaplasma-phagocytophilum-in-wild-cervids-and-hares-in-china
#10
Jifei Yang, Zhijie Liu, Qingli Niu, Jianxun Luo, Xiaolong Wang, Hong Yin
Anaplasma phagocytophilum is an emerging tick-borne pathogen of global veterinary and public health significance. Wild ungulates are suggested to serve as reservoirs for this agent in both the US and Europe. We investigated the occurrence of A. phagocytophilum in sika deer ( Cervus nippon ), Reeves' muntjac ( Muntiacus reevesi ), tufted deer ( Elaphodus cephalophus ), and Chinese hare ( Lepus sinensis ) in China. We detected A. phagocytophilum in nine (53%) free-ranging wild Reeves' muntjac. No positive sample was found in wild tufted deer, hare, or domesticated sika deer...
February 2, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28143697/first-detection-of-tick-borne-pathogens-of-dogs-from-malta
#11
Elaine Licari, Nóra Takács, Norbert Solymosi, Róbert Farkas
The knowledge about the vector-borne infections in domestic dogs has been increasing worldwide. However no studies have been done on hard tick infestation and tick-borne pathogens (TBPs) of dogs present in Malta. Therefore, a total of 99 dogs was selected and inspected between March and July 2013 in 18 urban and 17 rural areas on the islands of Malta and Gozo. All ticks were removed from the dogs and identified. Blood samples were taken and tested for protozoa (Babesia spp. and Hepatozoon spp.) and bacteria (Anaplasma spp...
January 18, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28131594/detection-of-borrelia-miyamotoi-and-other-tick-borne-pathogens-in-human-clinical-specimens-and-ixodes-scapularis-ticks-in-new-york-state-2012-2015
#12
Danielle Wroblewski, Linda Gebhardt, Melissa A Prusinski, Lisa J Meehan, Tanya A Halse, Kimberlee A Musser
Borrelia miyamotoi (Bm) is a recently emerging bacterial agent transmitted by several species of ixodid ticks. Diagnosis of Bm infection can be challenging, as the organism is not easily cultivable. We have developed and validated a multiplex real-time PCR to simultaneously identify Bm infection and the agents causing human granulocytic anaplasmosis and human monocytic ehrlichiosis, Anaplasma phagocytophilum and Ehrlichia chaffeensis, respectively. The assay is 100% specific; highly sensitive, detecting 11 gene copies of Bm DNA in both whole blood and cerebral spinal fluid; and provides rapid results in less than two hours...
January 16, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28131376/molecular-evidence-of-tick-borne-hemoprotozoan-parasites-theileria-ovis-and-babesia-ovis-and-bacteria-in-ticks-and-blood-from-small-ruminants-in-northern-algeria
#13
Atef Aouadi, Hamza Leulmi, Mehdi Boucheikhchoukh, Ahmed Benakhla, Didier Raoult, Philippe Parola
Using qPCR, standard PCR and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria. Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28130168/anaplasma-platys-like-strains-in-ruminants-from-tunisia
#14
Mourad Ben Said, Hanène Belkahia, Narjesse El Mabrouk, Mariem Saidani, Alberto Alberti, Rosanna Zobba, Amal Cherif, Tarek Mahjoub, Ali Bouattour, Lilia Messadi
Molecular diagnosis of Anaplasma platys and related strains (A. platys-like) in carnivores and ruminants is challenging due to co-infections with cross-reacting strains, and require post-amplification sequencing of the hemi-nested PCR products traditionally generated by targeting the groEL gene. In this study, a Restriction Enzyme Fragment Length Polymorphism (RFLP) assay coupled to hemi-nested groEL PCR was developed to discriminate among A. platys and genetically related strains. This novel approach was used for investigating A...
January 24, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28122249/genetic-diversity-and-molecular-epidemiology-of-anaplasma
#15
REVIEW
Mara Battilani, Stefano De Arcangeli, Andrea Balboni, Francesco Dondi
Anaplasma are obligate intracellular bacteria of cells of haematopoietic origin and are aetiological agents of tick-borne diseases of both veterinary and medical interest common in both tropical and temperate regions. The recent disclosure of their zoonotic potential has greatly increased interest in the study of these bacteria, leading to the recent reorganisation of Rickettsia taxonomy and to the possible discovery of new species belonging to the genus Anaplasma. This review is particularly focused on the common and unique characteristics of Anaplasma marginale and Anaplasma phagocytophilum, with an emphasis on genetic diversity and evolution, and the main distinguishing features of the diseases caused by the different Anaplasma spp...
January 22, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28118097/a-loop-mediated-isothermal-amplification-lamp-assay-targeting-16s-rrna-gene-for-rapid-detection-of-anaplasma-phagocytophilum-infection-in-sheep-and-goats
#16
Changshen Ning, Jinhong Wang, Yan Zhang, Xiaoxing Wang, Yanyan Cui, Yaqun Yan, Rongjun Wang, Fuchun Jian, Longxian Zhang
Anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (HGA) in humans and tick-borne fever in various kinds of animals. In the present study, a loop-mediated isothermal amplification (LAMP) assay for rapid detection of A. phagocytophilum was developed using primers specific to 16S rRNA gene of this organism. The LAMP assay was performed at 65 C for 60 min and terminated at 80 C for 10 min. The optimal reaction conditions, under which no cross-reaction was observed with other closely related tick borne parasites (Anaplasma bovis, Anaplasma ovis, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum) was established...
January 24, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28109769/molecular-typing-and-diagnosis-of-anaplasma-spp-closely-related-to-anaplasma-phagocytophilum-in-ruminants-from-tunisia
#17
Mourad Ben Said, Hanène Belkahia, Narjesse El Mabrouk, Mariem Saidani, Mariem Ben Hassen, Alberto Alberti, Rosanna Zobba, Skander Bouattour, Ali Bouattour, Lilia Messadi
Accurate diagnosis of animal and zoonotic diseases, such as granulocytic anaplasmosis, is crucial to estimate risk during control programs. In this study, 16S rRNA nested PCR and RFLP assay were combined to investigate the presence of Anaplasma phagocytophilum and genetically related strains (namely A. phagocytophilum-like 1 and 2) in 936 Tunisian ruminants. By using this method, A. phagocytophilum was not detected in any of the tested animals, while A. phagocytophilum-like 1 and A. phagocytophilum-like 2 were detected at variable prevalence rates in sheep, goats and cattle at coinfection rates respectively of 3...
January 16, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28096621/multiple-infections-of-anaplasma-platys-variants-in-philippine-dogs
#18
Adrian Patalinghug Ybañez, Rochelle Haidee Daclan Ybañez, Naoaki Yokoyama, Hisashi Inokuma
AIM: Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. To provide molecular evidence on the multiple infections of A. platys variants in Philippine dogs. MATERIALS AND METHODS: DNA fragments of A. platys from infected dogs in the Philippines were molecularly characterized. For screening, 25 dogs suspected to have canine anaplasmosis were tested using a 16S rRNA-based nested polymerase chain reaction (PCR)...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28096373/pathogen-mediated-manipulation-of-arthropod-microbiota-to-promote-infection
#19
Nabil M Abraham, Lei Liu, Brandon Lyon Jutras, Akhilesh K Yadav, Sukanya Narasimhan, Vissagan Gopalakrishnan, Juliana M Ansari, Kimberly K Jefferson, Felipe Cava, Christine Jacobs-Wagner, Erol Fikrig
Arthropods transmit diverse infectious agents; however, the ways microbes influence their vector to enhance colonization are poorly understood. Ixodes scapularis ticks harbor numerous human pathogens, including Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis. We now demonstrate that A. phagocytophilum modifies the I. scapularis microbiota to more efficiently infect the tick. A. phagocytophilum induces ticks to express Ixodes scapularis antifreeze glycoprotein (iafgp), which encodes a protein with several properties, including the ability to alter bacterial biofilm formation...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28093540/human-granulocytic-anaplasmosis-as-a-cause-of-febrile-illness-in-korea-since-at-least-2006
#20
Jongyoun Yi, Kye-Hyung Kim, Mee Kyung Ko, Eun Yup Lee, Su Jin Choi, Myoung-Don Oh
Human granulocytic anaplasmosis (HGA) is a tick-borne rickettsial zoonosis with fever, thrombocytopenia, and leukopenia. HGA has been reported in Korea in 2013 but it is uncertain how long it has existed. A retrospective study was conducted on patients who underwent bone marrow examination due to fever and cytopenia, with no clear hematologic or microbiologic causes, from 2003 through 2012. Laboratory diagnosis was made by detecting 16S rRNA genes of Anaplasma phagocytophilum from the stored blood samples. Among the 70 patients, five (7...
January 16, 2017: American Journal of Tropical Medicine and Hygiene
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