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Ms thyroid

Kerry J Welsh, Steven J Soldin
Hypothyroidism is a very common disorder worldwide, for which the usual treatment is monotherapy with levothyroxine (L-T4). However, a number of patients treated with L-T4 continue to report symptoms of hypothyroidism despite seemingly normal levels of thyroid-stimulating hormone (TSH), free-T3 (FT3) and free-T4 (FT4) measured by immunoassay. This review summarizes the limitations of the immunoassays commonly used to measure thyroid hormone levels and emphasizes the advantages of the role of liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
December 2016: European Journal of Endocrinology
Umal Azmat, Kyle Porter, Leigha Senter, Matthew D Ringel, Fadi Nabhan
BACKGROUND: Thyroglobulin (Tg) measurement in patients with positive anti-thyroglobulin antibodies (anti-Tg Abs) is not reliable. Tg measurement using Liquid Chromatography Tandem Mass Spectrometry (LC/MS) may be useful in this setting Methods: This is a retrospective study with the objective to determine the accuracy of Tg-LC/MS in patients with thyroid cancer with anti-Tg Abs. We evaluated all patients with follicular cell-derived thyroid cancer (TC) who had thyroglobulin measured using LC/MS assay from 11/1/2013-11/7/2014...
October 13, 2016: Thyroid: Official Journal of the American Thyroid Association
Kostja Renko, Carolin S Hoefig, Corinne Dupuy, Lisbeth Harder, Christian Schwiebert, Josef Köhrle, Lutz Schomburg
Iodotyrosine deiodinase (DEHAL1) is a crucial enzyme in iodine homeostasis. Unbound mono- and diiodotyrosines are indispensable byproducts of thyroid hormone biosynthesis. Their iodine needs to be recovered to avoid iodine deficiency, as observed in genetic defects in DEHAL1. Despite its importance, the enzyme is rarely studied. The deiodination process can be monitored by radioactive tracers or via techniques involving mass spectrometry (MS). However, isotope-labeled molecules are expensive, not always commercially available, and their use is legally restricted, while MS requires sophisticated, costly and sensitive instrumentation...
October 12, 2016: Endocrinology
Radu Campean, Matthias Hasun, Claudia Stöllberger, Johannes Bucher, Josef Finsterer, Christoph Schnack, Franz Weidinger
BACKGROUND: Reversible left ventricular dysfunction, also termed Takotsubo cardiomyopathy, is rarely reported in Addison's disease after initiation of hormone replacement therapy. The pathogenesis of this cardiomyopathy is unknown. CASE PRESENTATION: A 41-year-old white woman with a history of autoimmune Hashimoto thyroiditis diagnosed 3 years earlier and acute adrenal insufficiency diagnosed 3 weeks earlier presented with new onset of heart failure New York Heart Association class IV, which had started shortly after initiation of hormone replacement therapy with hydrocortisone 20 mg/day and fludrocortisone 0...
October 12, 2016: Journal of Medical Case Reports
Yuqian Luo, Takeshi Akama, Akiko Okayama, Aya Yoshihara, Mariko Sue, Kenzaburo Oda, Moyuru Hayashi, Yuko Ishido, Hisashi Hirano, Naoki Hiroi, Ryohei Katoh, Koichi Suzuki
BACKGROUND: Thyroglobulin (Tg) stored in thyroid follicles regulates follicular function in thyroid hormone (TH) synthesis by suppressing thyroid-specific gene expression in a concentration-dependent manner. Thus, Tg is an intrinsic negative-feedback regulator that can restrain the effect of thyroid stimulating hormone (TSH) in the follicle. However, the underlying mechanisms by which Tg exerts its prominent autoregulatory effect following recognition by thyrocytes remains unclear. METHODS: To identify potential proteins that recognize and interact with Tg, mass spectrometry (MS) was used to analyze immunoprecipitated Tg-bound proteins derived from Tg-treated rat thyroid FRTL-5 cells...
September 27, 2016: Thyroid: Official Journal of the American Thyroid Association
Jialing Zhang, Wendong Yu, Seung Ryu, John Lin, Gerardo Buentello, Robert Tibshirani, James Suliburk, Livia Schiavinato Eberlin
Oncocytic tumors are characterized by an excessive eosinophilic, granular cytoplasm due to aberrant accumulation of mitochondria. Mutations in mitochondrial DNA occurs in oncocytic thyroid tumors, but there is no information about their lipid composition which might reveal candidate theranostic molecules. Here we used desorption electrospray ionization mass spectrometry (DESI-MS) to image and chemically characterize the lipid composition of oncocytic thyroid tumors, as compared to non-oncocytic thyroid tumors and normal thyroid samples...
September 22, 2016: Cancer Research
Carolin S Hoefig, Riccardo Zucchi, Josef Koehrle
Thyronamines (3-T1AM, T0AM) are endogenous compounds probably derived from T4 or its intermediate metabolites. Combined activities of intestinal deiodinases and ornithine decarboxylase generate 3-T1AM in vitro. Alternatively, 3-T1AM might be formed by the thyroid gland and secreted into the blood. 3-T1AM and T0AM concentrations have been determined by liquid chromatography-tandem mass spectrometry analysis (LC-MS/MS) in tissues, serum and cell lines. However, large variations of 3-T1AM concentrations in human serum were reported by LC-MS/MS compared to a monoclonal antibody-based immunoassay...
September 21, 2016: Thyroid: Official Journal of the American Thyroid Association
Meri De Angelis, Florian Giesert, Brian Finan, Christoffer Clemmensen, Timo D Müller, Daniela Vogt-Weisenhorn, Matthias H Tschöp, Karl-Werner Schramm
Thyroid hormones (THs) play a critical role in the regulation of many biological processes such as growth, metabolism and development both in humans and wildlife. In general, TH levels are measured by immunoassay (IA) methods but the specificity of the antibodies used in these assays limits selectivity. In the last decade, several analytical methods using liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (LC-MS/MS) have been developed to measure THs. These new techniques proved to be more accurate than the IA analysis and they were widely used for the determination of TH level in different human and animal tissues...
October 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Steve Malangone, Christopher J Campen
A 60-year-old man initially presented with pain in the right upper quadrant in October 2010. A computed tomography (CT) scan of the abdomen pelvis completed at that time showed a mass at the junction of the body and tail of the pancreas and multiple large liver lesions. A CT-guided liver biopsy revealed low-grade neuroendocrine carcinoma. The patient was initially started on systemic treatment with sunitinib (Sutent) and octreotide. He developed intolerable side effects, including nausea and migraine. Therapy was discontinued in October 2011, when a CT scan revealed evidence of disease progression...
November 2015: Journal of the Advanced Practitioner in Oncology
Fahd A Al-Khamis
OBJECTIVES: To determine the relationship between Vitamin B12 levels and thyroid hormones in patients with multiple sclerosis (MS). MATERIALS AND METHODS: One hundred and ten patients with MS were recruited for this study after Institutional Review Board approval. All patients signed a written informed consent form and donated a single blood sample. Plasma Vitamin B12 levels, triiodothyronine (T3), and thyroxine (T4) hormone levels were measured. Data were analyzed using the Statistical Package for Social Sciences (SPSS) software...
September 2016: Journal of Family & Community Medicine
Karen D Lariosa-Willingham, Elen S Rosler, Jay S Tung, Jason C Dugas, Tassie L Collins, Dmitri Leonoudakis
BACKGROUND: Multiple sclerosis is caused by an autoimmune response resulting in demyelination and neural degeneration. The adult central nervous system has the capacity to remyelinate axons in part through the generation of new oligodendrocytes (OLs). To identify clinical candidate compounds that may promote remyelination, we have developed a high throughput screening (HTS) assay to identify compounds that promote the differentiation of oligodendrocyte precursor cells (OPCs) into OLs...
2016: BMC Research Notes
Yanjing Cao, Qing Guan, Tuanqi Sun, Wanshu Qi, Yinlong Guo
N-(1-chloroalkyl)pyridinium quaternization was developed for the derivatization of fatty aldehydes. Differing from common pre-charged reagents, non-charged pyridine and thionyl chloride were designed to add permanently charged tag on aldehydes. Pyridine was far less competitive than charged derivatives in ionization. Thionyl chloride in excess was quenched by deionized water, converting into less residual sulfur dioxide bubbles. Thus solutions could be tested directly by mass spectrometry without further post-treatments...
September 21, 2016: Analytica Chimica Acta
Muhammed Emin Ozcan, Bahri Ince, Hasan Huseyin Karadeli, Asuman Gedikbasi, Talip Asil, Meric A Altinoz
OBJECTIVE: To define whether minor adult hemoglobin A2 (HbA2, α2δ2) exerts any protective activity in multiple sclerosis (MS). METHODS: HbA2 levels were measured in 146 MS patients with high performance liquid chromatography and association with MS Severity Scores (MSSS) were determined. HbA2 associations with blood count parameters were also studied using blood counts evaluated on the same day of high performance liquid chromatography sampling. Routine biochemical parameters were also determined to rule out elusively influential factors, such as anemia and thyroid disorders...
2016: Neuropsychiatric Disease and Treatment
Shuai Meng, Wen Zhang, Li-Juan Guan, Fatuma-Said Muhali, Jiao-Zhen Zhou, Rong-Hua Song, Jian Xu, Jin-An Zhang
OBJECTIVES: To explore the proteomic changes in thyroid tissue from GD patients and find new biomarkers for the prevention, diagnosis as well as the treatment of GD. DESIGN AND METHODS: Group1 included five thyroid specimens of GD cases and 5 normal thyroid tissue samples which were removed surgically and collected. The proteins were extracted from these thyroid tissues and then the differentially expressed protein spots were identified by MALDI-TOF-MS. The interested proteins were further validated in more specimens (group2: 11 pathological thyroid specimens and 7 normal thyroid tissue samples)...
August 24, 2016: Clinical Biochemistry
Alejandra Serrano, Fátima Nogales, Paula Sobrino, María Luisa Murillo, Olimpia Carreras, María Luisa Ojeda
SCOPE: Cardiac hypertrophy is the greatest complication in metabolic syndrome (MS), in dams and in offspring. The most effective therapies to avoid the evolution of MS are anti-oxidants, anti-inflammatories, and insulin sensitizers. Among anti-oxidant elements, Selenium (Se) exerts its functions through selenoproteins, which are essential for the correct functioning of the cardiovascular system. The aim of the study is analyze selenoproteins' implication in the transmission of future cardiovascular problems to MS progeny...
August 13, 2016: Molecular Nutrition & Food Research
Xiaowen Zhang, Chenyan Li, Jinyuan Mao, Weiwei Wang, Xiaochen Xie, Shiqiao Peng, Zhaojun Wang, Cheng Han, Xiaomei Zhang, Danyang Wang, Chenling Fan, Zhongyan Shan, Weiping Teng
OBJECTIVES: To describe the changes in thyroglobulin (Tg) based upon gestational and postpartum concentrations in healthy pregnant women from an iodine-sufficient region in China, and to evaluate the use of Tg as a biomarker for iodine-sufficient pregnant women. DESIGN: A longitudinal study of Tg change in normal pregnant women from an iodine-sufficient region. PATIENTS AND MEASUREMENTS: Blood and urine samples were obtained from 133 pregnant women...
August 13, 2016: Clinical Endocrinology
Elżbieta Wieczorek, Sylwia Kędracka-Krok, Katarzyna Sołtys, Urszula Jankowska, Rafał Hołubowicz, Justyna Seliga, Andrzej Ożyhar
Ageing and mutations of transthyretin (TTR), the thyroid hormones and retinol transporting protein lead to amyloidosis by destabilizing the structure of TTR. Because protein structure is regulated through posttranslational modifications, we investigated the Small Ubiquitin-like Modifier (SUMO)ylation of TTR. We chose the widely used Ubc9 fusion-directed SUMOylation system, which is based on a fusion of the SUMOylation substrate of interest with Ubc9, a sole SUMO conjugating enzyme. Surprisingly, despite our presumptions, we found that Ubc9 fused to TTR was SUMOylated at a unique set of lysine residues...
2016: PloS One
Mustafa Çakar, Süleyman Metin, Şevket Balta, Cengiz Öztürk, Sait Demirkol, Tolga Çakmak, Satılmış İnal, Turgay Çelik, Atilla İyisoy, Murat Ünlü, Ahmet Şen
OBJECTIVES: For the purpose of flight safety military aircrew must be healthy. P-wave dispersion (PWD) is the p-wave length difference in an electrocardiographic (ECG) examination and represents the risk of developing atrial fibrillation. In the study we aimed at investigating PWD in healthy military aircrew who reported for periodical examinations. MATERIAL AND METHODS: Seventy-five asymptomatic military aircrew were enrolled in the study. All the subjects underwent physical, radiologic and biochemical examinations, and a 12-lead electrocardiography...
2016: International Journal of Occupational Medicine and Environmental Health
Brigitte Farizon, Marie Gavid, Alexandre Karkas, Jean-Marc Dumollard, Michel Peoc'h, Jean-Michel Prades
The aim of the present study was to evaluate the thyroarytenoid muscle response during bilateral thyroid surgery using vagal nerve stimulation. 195 patients (390 nerves at risk) underwent a total thyroidectomy. The recurrent laryngeal nerve's function was checked by analyzing the amplitude and the latency of the thyroarytenoid muscle's responses after a vagal nerve's stimulation (0.5 and 1 mA) using the NIM3 Medtronic system. All patients were submitted to preoperative and postoperative laryngoscopy. 20 patients get no thyroarytenoid muscle response to the vagal nerve stimulation, and 14 postoperative recurrent laryngeal nerve palsies were confirmed (3...
July 15, 2016: European Archives of Oto-rhino-laryngology
Gregory W Randolph, Dipti Kamani
OBJECTIVES: The electrophysiologic responses of 1,381 recurrent laryngeal nerves (RLN) during monitored neck surgery were recorded and reviewed. STUDY DESIGN: Retrospective case series. METHODS: With institutional review board approval, we reviewed thyroid and other neck surgeries performed with intraoperative nerve monitoring (IONM) between the years 1995 and 2006. This list yielded consecutively monitored 1,381 RLNs, with over 3,000 hours of monitoring experience...
July 8, 2016: Laryngoscope
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