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Engineered myocardium

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https://www.readbyqxmd.com/read/27909510/interactive-in-vitro-training-in-physics-of-radiofrequency-ablation-for-physicians-and-medical-engineering-students
#1
REVIEW
Haber T, Kleister G, Selman B, Härtig J, Melichercik J, Ismer B
Radiofrequency (RF) ablation requires a complex set of devices as well as profound electrophysiological experience and substantial knowledge of physical science basics. To establish RF ablation in-vitro teaching-system, six workstations were equipped with computer-controlled RF ablation generators. Universal connection boxes allow ablation-essays with catheters of different make and model. Special wetlabs were developed combining a basin containing isotonic saline solution with a thermostat and a pump to simulate blood flow...
June 2016: Journal of Atrial Fibrillation
https://www.readbyqxmd.com/read/27869075/-cell-therapies-for-cardiopathies-the-shift-of-paradigms
#2
Jean-Thomas Vilquin, Jessy Etienne
Heart failure is a major concern for public health systems, and several approaches of cellular therapy are being investigated with the goal of improving the function of these failing hearts. Many cell types have been used (skeletal myoblasts, hematopoietic, endothelial or mesenchymal progenitors, cardiac cells…), most often in the indication of post-ischemic heart failure rather than in the indication of genetic dilated cardiomyopathy. It is easier, indeed, to target a restricted area than the whole myocardium...
November 2016: Médecine Sciences: M/S
https://www.readbyqxmd.com/read/27834123/translational-applications-of-tissue-engineering-in-cardiovascular-medicine
#3
Y Murat Elçin, Arin Dogan, Eser Elçin
Cardiovascular diseases are the leading cause of worldwide deaths. Current paradigm in medicine seeks novel approaches for the treatment of progressive or end-stage diseases. The organ transplantation option is limited in availability and unfortunately, a great number of patients are lost while waiting for donor organs. Animal studies have shown that upon myocardial infarction, it may be possible to stop adverse remodeling in its tracks and reverse with tissue engineering methods. Regaining the myocardium function and avoiding further deterioration towards heart failure can benefit millions of people with a significantly lesser burden on healthcare systems worldwide...
November 11, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27833567/myofibroblasts-electrotonically-coupled-to-cardiomyocytes-alter-conduction-insights-at-the-cellular-level-from-a-detailed-in-silico-tissue-structure-model
#4
Florian Jousset, Ange Maguy, Stephan Rohr, Jan P Kucera
Fibrotic myocardial remodeling is typically accompanied by the appearance of myofibroblasts (MFBs). In vitro, MFBs were shown to slow conduction and precipitate ectopic activity following gap junctional coupling to cardiomyocytes (CMCs). To gain further mechanistic insights into this arrhythmogenic MFB-CMC crosstalk, we performed numerical simulations in cell-based high-resolution two-dimensional tissue models that replicated experimental conditions. Cell dimensions were determined using confocal microscopy of single and co-cultured neonatal rat ventricular CMCs and MFBs...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27826001/fibers-for-hearts-a-critical-review-on-electrospinning-for-cardiac-tissue-engineering
#5
REVIEW
Maria Kitsara, Onnik Agbulut, Dimitrios Kontziampasis, Yong Chen, Philippe Menasché
Cardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells. The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth...
November 5, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27822229/micro-and-macrostructured-plga-gelatin-scaffolds-promote-early-cardiogenic-commitment-of-human-mesenchymal-stem-cells-in-vitro
#6
Caterina Cristallini, Elisa Cibrario Rocchietti, Mariacristina Gagliardi, Leonardo Mortati, Silvia Saviozzi, Elena Bellotti, Valentina Turinetto, Maria Paola Sassi, Niccoletta Barbani, Claudia Giachino
The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineering still faces significant difficulties and challenges in the design of bioactive scaffolds, which allow composition variation to accommodate divergence in the evolving myocardial structure. Here we aimed at verifying if a microstructured bioartificial scaffold alone can provoke an effect on stem cell behavior...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27737958/mechanical-stress-conditioning-and-electrical-stimulation-promote-contractility-and-force-maturation-of-induced-pluripotent-stem-cell-derived-human-cardiac-tissue
#7
Jia-Ling Ruan, Nathaniel L Tulloch, Maria V Razumova, Mark Saiget, Veronica Muskheli, Lil Pabon, Hans Reinecke, Michael Regnier, Charles E Murry
BACKGROUND: Tissue engineering enables the generation of functional human cardiac tissue with cells derived in vitro in combination with biocompatible materials. Human-induced pluripotent stem cell-derived cardiomyocytes provide a cell source for cardiac tissue engineering; however, their immaturity limits their potential applications. Here we sought to study the effect of mechanical conditioning and electric pacing on the maturation of human-induced pluripotent stem cell-derived cardiac tissues...
November 15, 2016: Circulation
https://www.readbyqxmd.com/read/27729419/injectable-shear-thinning-hydrogels-for-minimally-invasive-delivery-to-infarcted-myocardium-to-limit-left-ventricular-remodeling
#8
Christopher B Rodell, Madonna E Lee, Hua Wang, Satoshi Takebayashi, Tetsushi Takayama, Tomonori Kawamura, Jeffrey S Arkles, Neville N Dusaj, Shauna M Dorsey, Walter R T Witschey, James J Pilla, Joseph H Gorman, Jonathan F Wenk, Jason A Burdick, Robert C Gorman
BACKGROUND: Injectable, acellular biomaterials hold promise to limit left ventricular remodeling and heart failure precipitated by infarction through bulking or stiffening the infarct region. A material with tunable properties (eg, mechanics, degradation) that can be delivered percutaneously has not yet been demonstrated. Catheter-deliverable soft hydrogels with in vivo stiffening to enhance therapeutic efficacy achieve these requirements. METHODS AND RESULTS: We developed a hyaluronic acid hydrogel that uses a tandem crosslinking approach, where the first crosslinking (guest-host) enabled injection and localized retention of a soft (<1 kPa) hydrogel...
October 2016: Circulation. Cardiovascular Interventions
https://www.readbyqxmd.com/read/27723557/dynamic-culture-yields-engineered-myocardium-with-near-adult-functional-output
#9
Christopher P Jackman, Aaron L Carlson, Nenad Bursac
Engineered cardiac tissues hold promise for cell therapy and drug development, but exhibit inadequate function and maturity. In this study, we sought to significantly improve the function and maturation of rat and human engineered cardiac tissues. We developed dynamic, free-floating culture conditions for engineering "cardiobundles", 3-dimensional cylindrical tissues made from neonatal rat cardiomyocytes or human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) embedded in fibrin-based hydrogel. Compared to static culture, 2-week dynamic culture of neonatal rat cardiobundles significantly increased expression of sarcomeric proteins, cardiomyocyte size (∼2...
December 2016: Biomaterials
https://www.readbyqxmd.com/read/27710832/bioprinting-3d-microfibrous-scaffolds-for-engineering-endothelialized-myocardium-and-heart-on-a-chip
#10
Yu Shrike Zhang, Andrea Arneri, Simone Bersini, Su-Ryon Shin, Kai Zhu, Zahra Goli-Malekabadi, Julio Aleman, Cristina Colosi, Fabio Busignani, Valeria Dell'Erba, Colin Bishop, Thomas Shupe, Danilo Demarchi, Matteo Moretti, Marco Rasponi, Mehmet Remzi Dokmeci, Anthony Atala, Ali Khademhosseini
Engineering cardiac tissues and organ models remains a great challenge due to the hierarchical structure of the native myocardium. The need of integrating blood vessels brings additional complexity, limiting the available approaches that are suitable to produce integrated cardiovascular organoids. In this work we propose a novel hybrid strategy based on 3D bioprinting, to fabricate endothelialized myocardium. Enabled by the use of our composite bioink, endothelial cells directly bioprinted within microfibrous hydrogel scaffolds gradually migrated towards the peripheries of the microfibers to form a layer of confluent endothelium...
December 2016: Biomaterials
https://www.readbyqxmd.com/read/27686755/electromechanical-conditioning-of-adult-progenitor-cells-improves-recovery-of-cardiac-function-after-myocardial-infarction
#11
Aida Llucià-Valldeperas, Carolina Soler-Botija, Carolina Gálvez-Montón, Santiago Roura, Cristina Prat-Vidal, Isaac Perea-Gil, Benjamin Sanchez, Ramon Bragos, Gordana Vunjak-Novakovic, Antoni Bayes-Genis
: : Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue-engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time...
September 29, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27637492/human-ips-cell-derived-cardiac-tissue-sheets-a-platform-for-cardiac-regeneration
#12
REVIEW
Hidetoshi Masumoto, Jun K Yamashita
Stem cell therapy is a promising therapeutic option for severe cardiac diseases that are resistant to conventional therapies. To overcome the unsatisfactory results of most clinical researches on stem cell injections to an injured heart, we are developing bioengineered cardiac tissue grafts using pluripotent stem cell-derived cardiomyocytes and vascular cells. We have validated the functional benefits of mouse embryonic stem cell-derived and human induced pluripotent stem cell-derived cardiac tissue sheets (CTSs) in a rat myocardial infarction model...
November 2016: Current Treatment Options in Cardiovascular Medicine
https://www.readbyqxmd.com/read/27624357/the-extracellular-matrix-patch-implanted-in-the-right-ventricle-evaluated-with-cardiovascular-magnetic-resonance-protocol-to-assess-regional-physio-mechanical-properties
#13
Akiko Tanaka, Keigo Kawaji, Amit R Patel, Takeyoshi Ota
OBJECTIVES: An extracellular matrix patch was implanted in the porcine right ventricle for in situ myocardial regeneration. A newly developed cardiovascular magnetic resonance protocol was utilized to investigate the regional physio-mechanical function of the patch. METHODS: Cardiovascular magnetic resonance was performed at 60-day after the porcine right ventricular wall full thickness substitution with an extracellular matrix cardiac patch (n = 5). Dacron patches and remote normal right ventricle served as control (n = 5/each)...
September 13, 2016: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/27572147/anisotropic-engineered-heart-tissue-made-from-laser-cut-decellularized-myocardium
#14
Jonas Schwan, Andrea T Kwaczala, Thomas J Ryan, Oscar Bartulos, Yongming Ren, Lorenzo R Sewanan, Aaron H Morris, Daniel L Jacoby, Yibing Qyang, Stuart G Campbell
We have developed an engineered heart tissue (EHT) system that uses laser-cut sheets of decellularized myocardium as scaffolds. This material enables formation of thin muscle strips whose biomechanical characteristics are easily measured and manipulated. To create EHTs, sections of porcine myocardium were laser-cut into ribbon-like shapes, decellularized, and mounted in specialized clips for seeding and culture. Scaffolds were first tested by seeding with neonatal rat ventricular myocytes. EHTs beat synchronously by day five and exhibited robust length-dependent activation by day 21...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27539859/peptidase-inhibitor-16-is-a-membrane-tethered-regulator-of-chemerin-processing-in-the-myocardium
#15
Michael Regn, Bernhard Laggerbauer, Claudia Jentzsch, Deepak Ramanujam, Andrea Ahles, Sonja Sichler, Julia Calzada-Wack, Rory Koenen, Attila Braun, Bernhard Nieswandt, Stefan Engelhardt
A key response of the myocardium to stress is the secretion of factors with paracrine or endocrine function. Intriguing in this respect is peptidase inhibitor 16 (PI16), a member of the CAP family of proteins which we found to be highly upregulated in cardiac disease. Up to this point, the mechanism of action and physiological function of PI16 remained elusive. Here, we show that PI16 is predominantly expressed by cardiac fibroblasts, which expose PI16 to the interstitium via a glycophosphatidylinositol-(GPI) membrane anchor...
August 15, 2016: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/27525229/infection-resistant-mri-visible-scaffolds-for-tissue-engineering-applications
#16
Morteza Mahmoudi, Mingming Zhao, Yuka Matsuura, Sophie Laurent, Phillip C Yang, Daniel Bernstein, Pilar Ruiz-Lozano, Vahid Serpooshan
Tissue engineering utilizes porous scaffolds as template to guide the new tissue growth. Clinical application of scaffolding biomaterials is hindered by implant-associated infection and impaired in vivo visibility of construct in biomedical imaging modalities. We recently demonstrated the use of a bioengineered type I collagen patch to repair damaged myocardium. By incorporating superparamagnetic iron oxide nanoparticles into this patch, here, we developed an MRI-visible scaffold. Moreover, the embedded nanoparticles impeded the growth of Salmonella bacteria in the patch...
2016: BioImpacts: BI
https://www.readbyqxmd.com/read/27469308/engineered-mesenchymal-cell-based-patches-as-controlled-vegf-delivery-systems-to-induce-extrinsic-angiogenesis
#17
Stefano Boccardo, Emanuele Gaudiello, Ludovic Melly, Giulia Cerino, Davide Ricci, Ivan Martin, Friedrich Eckstein, Andrea Banfi, Anna Marsano
UNLABELLED: Therapeutic over-expression of Vascular Endothelial Growth Factor (VEGF) by transduced progenitors is a promising strategy to efficiently induce angiogenesis in ischemic tissues (e.g. limb muscle and myocardium), but tight control over the micro-environmental distribution of the dose is required to avoid induction of angioma-like tumors. Therapeutic VEGF release was achieved by purified transduced adipose mesenchymal stromal cells (ASC) that homogeneously produce specific VEGF levels, inducing only normal angiogenesis after injection in non-ischemic tissues...
September 15, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27435115/the-myocardial-regenerative-potential-of-three-dimensional-engineered-cardiac-tissues-composed-of-multiple-human-ips-cell-derived-cardiovascular-cell-lineages
#18
Hidetoshi Masumoto, Takeichiro Nakane, Joseph P Tinney, Fangping Yuan, Fei Ye, William J Kowalski, Kenji Minakata, Ryuzo Sakata, Jun K Yamashita, Bradley B Keller
Human induced pluripotent stem cells (hiPSCs) are a robust source for cardiac regenerative therapy due to their potential to support autologous and allogeneic transplant paradigms. The in vitro generation of three-dimensional myocardial tissue constructs using biomaterials as an implantable hiPSC-derived myocardium provides a path to realize sustainable myocardial regeneration. We generated engineered cardiac tissues (ECTs) from three cellular compositions of cardiomyocytes (CMs), endothelial cells (ECs), and vascular mural cells (MCs) differentiated from hiPSCs...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27384932/tissue-engineering-nano-enabled-approaches-for-stem-cell-based-cardiac-tissue-engineering-adv-healthcare-mater-13-2016
#19
Mahshid Kharaziha, Adnan Memic, Mohsen Akbari, David A Brafman, Mehdi Nikkhah
On page 1533 M. Nikkhah and co-workers review tissue engineering strategies in the treatment of cardiac diseases with emphasis on the use of nanoenabled approaches (e.g. nanoparticles, nanotopographies) in combination with stem cells for regeneration and repair of injured myocardium upon myocardial infarction.
July 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27272902/the-cytoprotective-capacity-of-processed-human-cardiac-extracellular-matrix
#20
Benjamin Kappler, Petra Anic, Matthias Becker, Andreas Bader, Kristin Klose, Oliver Klein, Barbara Oberwallner, Yeong-Hoon Choi, Volkmar Falk, Christof Stamm
Freshly isolated human cardiac extracellular matrix sheets (cECM) have been shown to support stem cell proliferation and tissue-specific lineage commitment. We now developed a protocol for standardized production of durable, bio-functional hcECM microparticles and corresponding hydrogel, and tested its cytoprotective effects on contractile cells subjected to ischemia-like conditions. Human ventricular myocardium was decellularized by a 3-step protocol, including Tris/EDTA, SDS and serum incubation (cECM). Following snap-freezing and lyophilization, microparticles were created and characterized by laser diffraction, dynamic image analysis (DIA), and mass spectrometry...
July 2016: Journal of Materials Science. Materials in Medicine
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