Alyssa Q Stiving, David J Foreman, Zachary L VanAernum, Eberhard Durr, Shiyi Wang, Josef Vlasak, Jennifer Galli, Jared O Kafader, Taku Tsukidate, Xuanwen Li, Hillary A Schuessler, Douglas D Richardson
Surface-embedded glycoproteins, such as the spike protein trimers of coronaviruses MERS, SARS-CoV, and SARS-CoV-2, play a key role in viral function and are the target antigen for many vaccines. However, their significant glycan heterogeneity poses an analytical challenge. Here, we utilized individual ion mass spectrometry (I2 MS), a multiplexed charge detection measurement with similarities to charge detection mass spectrometry (CDMS), in which a commercially available Orbitrap analyzer is used to directly produce mass profiles of these heterogeneous coronavirus spike protein trimers under native-like conditions...
November 30, 2023: Journal of the American Society for Mass Spectrometry