keyword
MENU ▼
Read by QxMD icon Read
search

Viral glycoprotein-D

keyword
https://www.readbyqxmd.com/read/28228587/prophylactic-herpes-simplex-virus-2-hsv-2-vaccines-adjuvanted-with-stable-emulsion-and-toll-like-receptor-9-agonist-induce-a-robust-hsv-2-specific-cell-mediated-immune-response-protect-against-symptomatic-disease-and-reduce-the-latent-viral-reservoir
#1
Michael T Hensel, Jason D Marshall, Michael R Dorwart, Darren S Heeke, Eileen Rao, Padmaja Tummala, Li Yu, Gary H Cohen, Roselyn J Eisenberg, Derek D Sloan
Several prophylactic vaccines targeting HSV-2 have failed in the clinic to demonstrate a sustained depression in viral shedding or protection from recurrences. Although these vaccines have generated high titers of neutralizing antibodies, their induction of robust CD8 T cells has largely been unreported, even though evidence for the importance of HSV-2 antigen-specific CD8 T cells is mounting in animal models and in translational studies involving subjects with active HSV-2-specific immune responses. We developed a subunit vaccine composed of the neutralizing antibody (nAb) targets gD and gB, the novel T cell antigen and tegument protein UL40, and we compared this to a whole-inactivated virus vaccine (FI-HSV-2)...
February 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28225652/dna-vaccination-partially-protects-muskellunge-against-viral-hemorrhagic-septicemia-virus-vhsv-ivb
#2
Elena V Millard, Ashley M Bourke, Scott E LaPatra, Travis O Brenden, Scott D Fitzgerald, Mohamed Faisal
A DNA vaccine containing the glycoprotein (G) gene of the North American viral hemorrhagic septicemia virus (VHSV) genotype IVb was developed to evaluate the immune response of fish following vaccination and evaluate its efficacy in protecting a susceptible species, the Muskellunge Esox masquinongy, against VHSV-IVb challenge. Seven weeks (539 degree-days) following vaccination with 10 μg of either pVHSivb-G or a control plasmid, Muskellunge were challenged by immersion with 10(5) plaque-forming units (pfu)/mL of VHSV-IVb...
March 2017: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/28194842/infectivity-inhibition-by-overlapping-synthetic-peptides-derived-from-the-gh-gl-heterodimer-of-herpes-simplex-virus-type-1
#3
Gianluigi Franci, Annarita Falanga, Carla Zannella, Veronica Folliero, Francesca Martora, Marilena Galdiero, Stefania Galdiero, Giancarlo Morelli, Massimiliano Galdiero
Herpes simplex virus (HSV) is a human pathogen that infects epithelial cells. The cutaneous lesions, caused by the virus, spread to the nervous system creating several complications. Fusion of host membranes with the viral envelope is mandatory and mediated by a group of glycoproteins conserved in all Herpesviridae subfamilies, such as the glycoproteins B (gB), H (gH), L (gL) and D (gD). We investigated the inhibitory activity mediated by synthetic overlapping peptides spanning the entire ectodomains of gH and gL glycoproteins...
February 14, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28179907/a-dna-vaccine-formulated-with-chemical-adjuvant-provides-partial-protection-against-bovine-herpes-virus-infection-in-cattle
#4
Valeria Quattrocchi, Ivana Soria, Cecilia Ana Langellotti, Victoria Gnazzo, Mariela Gammella, Dadin P Moore, Patricia I Zamorano
Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and also inactivated vaccines, are currently used in many countries, they have several disadvantages. DNA vaccines have emerged as an attractive approach since they have the potential to induce both humoral and cellular immune response; nevertheless, it is largely known that potency of naked DNA vaccines is limited...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28152326/safety-and-immunogenicity-of-gamevac-combi-a-heterologous-vsv-and-ad5-vectored-ebola-vaccine-an-open-phase-i-ii-trial-in-healthy-adults-in-russia
#5
I V Dolzhikova, O V Zubkova, A I Tukhvatulin, A S Dzharullaeva, N M Tukhvatulina, D V Shcheblyakov, M M Shmarov, E A Tokarskaya, Y V Simakova, D A Egorova, D N Scherbinin, I L Tutykhina, A A Lysenko, A V Kostarnoy, P G Gancheva, T A Ozharovskaya, B V Belugin, L V Kolobukhina, V B Pantyukhov, S I Syromyatnikova, I V Shatokhina, T V Sizikova, I G Rumyantseva, A F Andrus, N V Boyarskaya, A N Voytyuk, V F Babira, S V Volchikhina, D A Kutaev, A N Bel'skih, K V Zhdanov, S M Zakharenko, S V Borisevich, D Y Logunov, B S Naroditsky, A L Gintsburg
Ebola hemorrhagic fever, also known as Ebola virus disease or EVD, is one of the most dangerous viral diseases in humans and animals. In this open-label, dose-escalation clinical trial, we assessed the safety, side effects, and immunogenicity of a novel, heterologous prime-boost vaccine against Ebola, which was administered in 2 doses to 84 healthy adults of both sexes between 18 and 55 years. The vaccine consists of live-attenuated recombinant vesicular stomatitis virus (VSV) and adenovirus serotype-5 (Ad5) expressing Ebola envelope glycoprotein...
February 2, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28126619/enhancement-of-glycoprotein-based-dna-vaccine-for-viral-hemorrhagic-septicemia-virus-vhsv-via-addition-of-the-molecular-adjuvant-ddx41
#6
Jassy Mary S Lazarte, Young Rim Kim, Jung Seok Lee, Se Pyeong Im, Si Won Kim, Jae Wook Jung, Jaesung Kim, Woo Jai Lee, Tae Sung Jung
The use of molecular adjuvants to improve the immunogenicity of DNA vaccines has been thoroughly studied in recent years. Glycoprotein (G)-based DNA vaccines had been proven to be effective in combating infection against Rhabdovirus (especially infectious hematopoietic necrosis virus, IHNV) in salmonids. DDX41 is a helicase known to induce antiviral and inflammatory responses by inducing a type I IFN innate immune response. To gain more information regarding G-based DNA vaccines in olive flounder (Paralicthys olivaceus), we tried to develop a more efficient G-based DNA vaccine by adding a molecular adjuvant, DDX41...
January 23, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28119397/misdirection-of-endosomal-trafficking-mediated-by-herpes-simplex-virus-encoded-glycoprotein-b
#7
Naima Niazy, Sebastian Temme, Derya Bocuk, Carmen Giesen, Angelika König, Nadine Temme, Angelique Ziegfeld, Tone F Gregers, Oddmund Bakke, Thorsten Lang, Anna M Eis-Hübinger, Norbert Koch
Herpes simplex virus (HSV)-encoded glycoprotein B (gB) is the most abundant protein in the viral envelope and promotes fusion of the virus with the cellular membrane. In the present study, we found that gB impacts on the major histocompatibility complex (MHC)-II pathway of antigen presentation by fostering homotypic fusion of early endosomes and trapping MHC-II molecules in these altered endosomes. By using an overexpression approach, we demonstrated that transient expression of gB induces giant vesicles of early endosomal origin, which contained Rab5, early endosomal antigen 1 (EEA1), large amounts of MHC-II molecules [human leukocyte antigen (HLA)-DR, and HLA-DM] but no CD63...
January 24, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28065804/limited-naturally-occurring-escape-in-broadly-neutralizing-antibody-epitopes-in-hepatitis-c-glycoprotein-e2-and-constrained-sequence-usage-in-acute-infection
#8
Chaturaka Rodrigo, Melanie R Walker, Preston Leung, Auda A Eltahla, Jason Grebely, Gregory J Dore, Tanya Applegate, Kimberly Page, Sunita Dwivedi, Julie Bruneau, Meghan D Morris, Andrea L Cox, William Osburn, Arthur Y Kim, Janke Schinkel, Naglaa H Shoukry, Georg M Lauer, Lisa Maher, Margaret Hellard, Maria Prins, Fabio Luciani, Andrew R Lloyd, Rowena A Bull
Broadly neutralizing antibodies have been associated with spontaneous clearance of the hepatitis C infection as well as viral persistence by immune escape. Further study of neutralizing antibody epitopes is needed to unravel pathways of resistance to virus neutralization, and to identify conserved regions for vaccine design. All reported broadly neutralizing antibody (BNAb) epitopes in the HCV Envelope (E2) glycoprotein were identified. The critical contact residues of these epitopes were mapped onto the linear E2 sequence...
January 5, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28060576/evagreen-based-multiplex-real-time-pcr-assay-for-rapid-differentiation-of-wild-type-and-glycoprotein-e-deleted-bovine-herpesvirus-1-strains
#9
Sachin S Pawar, Chetan D Meshram, Niraj K Singh, Mohini Saini, B P Mishra, Praveen K Gupta
Bovine herpesvirus-1 (BoHV-1) is an important viral pathogen causing significant economic losses to the cattle industry. Glycoprotein E-deleted marker vaccines form the basis for BoHV-1 control programs widely, wherein detection and differentiation of wild-type and gE-deleted vaccine strains is of crucial importance for proper disease management. In the present study, we report an EvaGreen-based multiplex real-time polymerase chain reaction (EGRT-PCR) assay for rapid differentiation of wild-type and glycoprotein E-deleted strains of BoHV-1...
January 6, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28056966/pseudorabies-virus-infection-aujeszky-s-disease-in-an-iberian-lynx-lynx-pardinus-in-spain-a-case-report
#10
A Javier Masot, María Gil, David Risco, Olga M Jiménez, José I Núñez, Eloy Redondo
BACKGROUND: The only natural hosts of Pseudorabies virus (PRV) are members of the family Suidae (Sus scrofa scrofa). In species other than suids infection is normally fatal. In these mammals, including carnivores, PRV typically causes serious neurologic disease. The endangered Iberian lynx (Lynx pardinus) is a wild feline endemic to south-western Europe (Iberian Peninsula). The Iberian lynx was found to be the world's most endangered felid species in 2002. In wild felines, PRV infection has only been previously reported once in a Florida panther in 1994...
January 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/27992602/receptor-activation-of-hiv-1-env-leads-to-asymmetric-exposure-of-the-gp41-trimer
#11
Mukta D Khasnis, Konstantine Halkidis, Anshul Bhardwaj, Michael J Root
Structural rearrangements of HIV-1 glycoprotein Env promote viral entry through membrane fusion. Env is a symmetric homotrimer with each protomer composed of surface subunit gp120 and transmembrane subunit gp41. Cellular CD4- and chemokine receptor-binding to gp120 coordinate conformational changes in gp41, first to an extended prehairpin intermediate (PHI) and, ultimately, into a fusogenic trimer-of-hairpins (TOH). HIV-1 fusion inhibitors target gp41 in the PHI and block TOH formation. To characterize structural transformations into and through the PHI, we employed asymmetric Env trimers containing both high and low affinity binding sites for individual fusion inhibitors...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27981441/distribution-of-cellular-hsv-1-receptor-expression-in-human-brain
#12
Richard Lathe, Juergen G Haas
Herpes simplex virus type 1 (HSV-1) is a neurotropic virus linked to a range of acute and chronic neurological disorders affecting distinct regions of the brain. Unusually, HSV-1 entry into cells requires the interaction of viral proteins glycoprotein D (gD) and glycoprotein B (gB) with distinct cellular receptor proteins. Several different gD and gB receptors have been identified, including TNFRSF14/HVEM and PVRL1/nectin 1 as gD receptors and PILRA, MAG, and MYH9 as gB receptors. We investigated the expression of these receptor molecules in different areas of the adult and developing human brain using online transcriptome databases...
December 15, 2016: Journal of Neurovirology
https://www.readbyqxmd.com/read/27909702/complement-inhibition-enables-tumor-delivery-of-lcmv-glycoprotein-pseudotyped-viruses-in-the-presence-of-antiviral-antibodies
#13
Laura Evgin, Carolina S Ilkow, Marie-Claude Bourgeois-Daigneault, Christiano Tanese de Souza, Lawton Stubbert, Michael S Huh, Victoria A Jennings, Monique Marguerie, Sergio A Acuna, Brian A Keller, Charles Lefebvre, Theresa Falls, Fabrice Le Boeuf, Rebecca A Auer, John D Lambris, J Andrea McCart, David F Stojdl, John C Bell
The systemic delivery of therapeutic viruses, such as oncolytic viruses or vaccines, is limited by the generation of neutralizing antibodies. While pseudotyping of rhabdoviruses with the lymphocytic choriomeningitis virus glycoprotein has previously allowed for multiple rounds of delivery in mice, this strategy has not translated to other animal models. For the first time, we provide experimental evidence that antibodies generated against the lymphocytic choriomeningitis virus glycoprotein mediate robust complement-dependent viral neutralization via activation of the classical pathway...
2016: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/27868164/efficient-production-of-recombinant-glycoprotein-d-of-herpes-simplex-virus-type-2-in-pichia-pastoris-and-its-protective-efficacy-against-viral-challenge-in-mice
#14
Man Wang, Shuai Jiang, Li Zhou, Chaoqun Wang, Ruifeng Mao, Murugavel Ponnusamy
Herpes simplex virus type 2 (HSV-2) infection is the leading cause of genital ulcer disease and a significant public health concern. However, there are no approved vaccines available to prevent HSV-2 infection. The glycoprotein D (gD) of HSV-2 is the most important candidate antigen for vaccine development. In this study, a truncated form of gD (codons 1-340, gD1-340) was produced as a secretory protein in the methylotrophic yeast Pichia pastoris. The recombinant gD1-340 with a His6 tag was purified to homogeneity by one-step affinity chromatography...
November 21, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27814506/ebola-virus-glycoprotein-with-increased-infectivity-dominated-the-2013-2016-epidemic
#15
William E Diehl, Aaron E Lin, Nathan D Grubaugh, Luiz Max Carvalho, Kyusik Kim, Pyae Phyo Kyawe, Sean M McCauley, Elisa Donnard, Alper Kucukural, Patrick McDonel, Stephen F Schaffner, Manuel Garber, Andrew Rambaut, Kristian G Andersen, Pardis C Sabeti, Jeremy Luban
The magnitude of the 2013-2016 Ebola virus disease (EVD) epidemic enabled an unprecedented number of viral mutations to occur over successive human-to-human transmission events, increasing the probability that adaptation to the human host occurred during the outbreak. We investigated one nonsynonymous mutation, Ebola virus (EBOV) glycoprotein (GP) mutant A82V, for its effect on viral infectivity. This mutation, located at the NPC1-binding site on EBOV GP, occurred early in the 2013-2016 outbreak and rose to high frequency...
November 3, 2016: Cell
https://www.readbyqxmd.com/read/27789948/hepatitis-c-virus-e2-protein-encapsulation-into-poly-d-l-lactic-co-glycolide-microspheres-could-induce-mice-cytotoxic-t-cell-response
#16
Piyachat Roopngam, Kewei Liu, Lin Mei, Yi Zheng, Xianbing Zhu, Hsiang-I Tsai, Laiqiang Huang
Hepatitis C virus (HCV) is known to cause hepatitis and hepatocellular carcinoma. E2 envelope glycoprotein of HCV type (HCV-E2) has been reported to bind human host cells and is a major target for developing anti-HCV vaccines. However, the therapeutic vaccine for infected patients still needs further development. The vaccine aims to provide cytotoxic T-cells to eliminate infected cells and hepatocellular carcinoma. Currently, there is no effective HCV therapeutic vaccine because most chronically infected patients rarely generate cytotoxic T-cells, even though they have high levels of neutralizing antibodies...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27783711/mechanistic-insight-into-bunyavirus-induced-membrane-fusion-from-structure-function-analyses-of-the-hantavirus-envelope-glycoprotein-gc
#17
Pablo Guardado-Calvo, Eduardo A Bignon, Eva Stettner, Scott Allen Jeffers, Jimena Pérez-Vargas, Gerard Pehau-Arnaudet, M Alejandra Tortorici, Jean-Luc Jestin, Patrick England, Nicole D Tischler, Félix A Rey
Hantaviruses are zoonotic viruses transmitted to humans by persistently infected rodents, giving rise to serious outbreaks of hemorrhagic fever with renal syndrome (HFRS) or of hantavirus pulmonary syndrome (HPS), depending on the virus, which are associated with high case fatality rates. There is only limited knowledge about the organization of the viral particles and in particular, about the hantavirus membrane fusion glycoprotein Gc, the function of which is essential for virus entry. We describe here the X-ray structures of Gc from Hantaan virus, the type species hantavirus and responsible for HFRS, both in its neutral pH, monomeric pre-fusion conformation, and in its acidic pH, trimeric post-fusion form...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27783673/crystal-structure-of-glycoprotein-c-from-a-hantavirus-in-the-post-fusion-conformation
#18
Shmuel Willensky, Hagit Bar-Rogovsky, Eduardo A Bignon, Nicole D Tischler, Yorgo Modis, Moshe Dessau
Hantaviruses are important emerging human pathogens and are the causative agents of serious diseases in humans with high mortality rates. Like other members in the Bunyaviridae family their M segment encodes two glycoproteins, GN and GC, which are responsible for the early events of infection. Hantaviruses deliver their tripartite genome into the cytoplasm by fusion of the viral and endosomal membranes in response to the reduced pH of the endosome. Unlike phleboviruses (e.g. Rift valley fever virus), that have an icosahedral glycoprotein envelope, hantaviruses display a pleomorphic virion morphology as GN and GC assemble into spikes with apparent four-fold symmetry organized in a grid-like pattern on the viral membrane...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27764150/stability-characterization-of-a-vaccine-antigen-based-on-the-respiratory-syncytial-virus-fusion-glycoprotein
#19
Jessica A Flynn, Eberhard Durr, Ryan Swoyer, Pedro J Cejas, Melanie S Horton, Jennifer D Galli, Scott A Cosmi, Amy S Espeseth, Andrew J Bett, Lan Zhang
Infection with Respiratory Syncytial Virus (RSV) causes both upper and lower respiratory tract disease in humans, leading to significant morbidity and mortality in both young children and older adults. Currently, there is no licensed vaccine available, and therapeutic options are limited. During the infection process, the type I viral fusion (F) glycoprotein on the surface of the RSV particle rearranges from a metastable prefusion conformation to a highly stable postfusion form. In people naturally infected with RSV, most potent neutralizing antibodies are directed to the prefusion form of the F protein...
2016: PloS One
https://www.readbyqxmd.com/read/27733647/in-vivo-efficacy-of-measles-virus-fusion-protein-derived-peptides-is-modulated-by-the-properties-of-self-assembly-and-membrane-residence
#20
T N Figueira, L M Palermo, A S Veiga, D Huey, C A Alabi, N C Santos, J C Welsch, C Mathieu, B Horvat, S Niewiesk, A Moscona, M A R B Castanho, M Porotto
: Measles virus (MV) infection is undergoing resurgence and remains one of the leading causes of death among young children worldwide despite the availability of an effective measles vaccine. MV infects its target cells by coordinated action of the MV hemagglutinin (H) and fusion (F) envelope glycoproteins; upon receptor engagement by H, the prefusion F undergoes a structural transition, extending and inserting into the target cell membrane and then refolding into a postfusion structure that fuses the viral and cell membranes...
January 1, 2017: Journal of Virology
keyword
keyword
69196
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"